Publications

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the potential of antagonistic fungi for control of fusarium graminearum and fusarium crookwellense varies depending on the experimental approach.to investigate the potential of fungal antagonists to control fusarium head blight (fhb) causing pathogens (fusarium graminearum and f. crookwellense) with two different experimental approaches.201525692943
fungal antagonism assessment of predatory species and producers metabolites and their effectiveness on haemonchus contortus infective larvae.the objective of this study was to assess antagonism of nematophagous fungi and species producers metabolites and their effectiveness on haemonchus contortus infective larvae (l3). assay a assesses the synergistic, additive, or antagonistic effect on the production of spores of fungal isolates of the species duddingtonia flagrans, clonostachys rosea, trichoderma esau, and arthrobotrys musiformis; assay b evaluates in vitro the effect of intercropping of these isolates grown in 2% water-agar (2% ...201526504791
hazard identification of cis/trans-zearalenone through the looking-glass.among the food-related health issues, the presence of contaminants has a prominent role, due to the wide range of exogenous compounds that can occur in food commodities and to their large differences in structure and biological activity. a comprehensive assessment of the related risk is thus actually demanding in terms of time and facilities involved. in this context, the use of computational strategies can be an effective choice for supporting the hazard identification procedure at the early st ...201526391124
necrotrophic mycoparasites and their genomes.mycoparasitism is a lifestyle where one fungus establishes parasitic interactions with other fungi. species of the genus trichoderma together with clonostachys rosea are among the most studied fungal mycoparasites. they have wide host ranges comprising several plant pathogens and are used for biological control of plant diseases. trichoderma as well as c. rosea mycoparasites efficiently overgrow and kill their fungal prey by using infection structures and by applying lytic enzymes and toxic meta ...201728281442
draft genome sequence of mycoparasite clonostachys rosea strain 67-1.clonostachys rosea is a promising mycoparasite. in this study, we sequenced the draft genome of the highly effective strain 67-1 using the illumina hiseq 2500 sequencing platform. the genome is 55.4 mb with a g+c content of 49.2% and provides a powerful resource for future studies on the molecular mechanisms underlying clonostachys rosea's antagonism on fungal pathogens.201526021926
insect deterrent and nematicidal screening of microfungi from mexico and anti-aphid compounds from gliomastix masseei.fungal metabolites are promising alternatives for the development of biorational pesticides. in this sense, microfungi from tropical regions are valuable sources of natural compounds for pest management. with the aim of broadening the search for new eco-friendly products to manage plant pests, this study was carried out to evaluate the biological activity of 23 tropical fungal extracts on three species of phytophagous insects and a plant parasitic nematode. in addition, the active principles of ...201728258772
dual effects of metarhizium spp. and clonostachys rosea against an insect and a seed-borne pathogen in wheat.crops are often prone to both insect herbivory and disease, which necessitate multiple control measures. ideally, an efficacious biological control agent must adequately control the target organism and not be inhibited by other biological control agents when applied simultaneously. wheat seeds infected with the plant pathogen fusarium culmorum were treated with metarhizium brunneum or m. flavoviride and clonostachys rosea individually and in combination, with the expectation to control both root ...201625827357
insights on the evolution of mycoparasitism from the genome of clonostachys rosea.clonostachys rosea is a mycoparasitic fungus that can control several important plant diseases. here, we report on the genome sequencing of c. rosea and a comparative genome analysis, in order to resolve the phylogenetic placement of c. rosea and to study the evolution of mycoparasitism as a fungal lifestyle. the genome of c. rosea is estimated to 58.3 mb, and contains 14,268 predicted genes. a phylogenomic analysis shows that c. rosea clusters as sister taxon to plant pathogenic fusarium specie ...201525575496
diversity of clonostachys species assessed by molecular phylogenetics and maldi-tof mass spectrometry.we assessed the species diversity among 45 strains of clonostachys from different substrates and localities in brazil using molecular phylogenetics, and compared the results with the phenotypic classification of strains obtained from matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms). phylogenetic analyses were based on beta tubulin (tub), its-lsu rdna, and a combined tub-its dna dataset. maldi-tof ms analyses were performed using intact conidia and conid ...201425457948
spore production of clonostachys rosea in a new solid-state fermentation reactor.a new solid-state fermentation (ssf) reactor was developed for the spore production of biocontrol agent clonostachys rosea. the greatest spore production in the reactor, 3.36 × 10(10) spores g dm(-1) occurred with mixings, which was about 10 times greater than that in traditional tray reactor. the reactor provides about two times sporulation surface area for spore formation. moisture content of the medium was adjusted to meet the spore production by changing the surface porosity. two mixings wer ...201425267354
colonization of onions by endophytic fungi and their impacts on the biology of thrips tabaci.endophytic fungi, which live within host plant tissues without causing any visible symptom of infection, are important mutualists that mediate plant-herbivore interactions. thrips tabaci (lindeman) is one of the key pests of onion, allium cepa l., an economically important agricultural crop cultivated worldwide. however, information on endophyte colonization of onions, and their impacts on the biology of thrips feeding on them, is lacking. we tested the colonization of onion plants by selected f ...201425254657
synergistic effect of dazomet soil fumigation and clonostachys rosea against cucumber fusarium wilt.soil fumigation and biological control are two control measures frequently used against soilborne diseases. in this study, the chemical fumigant dazomet was applied in combination with the biocontrol agent (bca) clonostachys rosea 67-1 to combat cucumber wilt caused by fusarium oxysporum f. sp. cucumerinum kw2-1. when the mycoparasite c. rosea 67-1 was applied after dazomet fumigation, disease control reached 100%, compared with 88.1 and 69.8% for dazomet and 67-1 agent, respectively, applied al ...201424941326
investigating the compatibility of the biocontrol agent clonostachys rosea ik726 with prodigiosin-producing serratia rubidaea s55 and phenazine-producing pseudomonas chlororaphis toza7.this study was carried out to assess the compatibility of the biocontrol fungus clonostachys rosea ik726 with the phenazine-producing pseudomonas chlororaphis toza7 or with the prodigiosin-producing serratia rubidaea s55 against fusarium oxysporum f. sp. radicis-lycopersici. the pathogen was inhibited by both strains in vitro, whereas c. rosea displayed high tolerance to s. rubidaea but not to p. chlororaphis. we hypothesized that this could be attributed to the atp-binding cassette (abc) protei ...201626860841
zearalenone lactonohydrolase activity in hypocreales and its evolutionary relationships within the epoxide hydrolase subset of a/b-hydrolases.zearalenone is a mycotoxin produced by several species of fusarium genus, most notably fusarium graminearum and fusarium culmorum. this resorcylic acid lactone is one of the most important toxins causing serious animal and human diseases. for over two decades it has been known that the mycoparasitic fungus clonostachys rosea (synonym: gliocladium roseum, teleomorph: bionectria ochroleuca) can detoxify zearalenone, however no such attributes have been described within the trichoderma genus.201424708405
biological control as a strategy to reduce the impact of mycotoxins in peanuts, grapes and cereals in argentina.mycotoxins including aflatoxins, deoxynivalenol, fumonisins and ochratoxin a are among the main fungal secondary metabolites detected as natural contaminants in south america in different commodities such as peanuts (aflatoxins), cereals (deoxynivalenol and fumonisins) or grapes (ochratoxin a). different strategies including crop rotation, tillage practices, fungicide application and planting less susceptible cultivars are used in order to reduce the impact of these mycotoxins in both food and f ...201525427716
interactions among filamentous fungi aspergillus niger, fusarium verticillioides and clonostachys rosea: fungal biomass, diversity of secreted metabolites and fumonisin production.interactions among fungi colonizing dead organic matter involve exploitation competition and interference competition. major mechanism of interference competition is antibiosis caused by secreted secondary metabolites. the effect of competition on secondary metabolite production by fungi is however poorly understood. fungal biomass was rarely monitored in interaction studies; it is not known whether dominance in pairwise interactions follows congruent patterns.201627165654
incidence and severity of leaf and fruit diseases of plums in latvia.in the present study six plum orchards in latvia were examined during 2014. one orchard was commercial with integrated pest management (ipm) practices, one was with organic management, two orchards were scientific collections and in two orchards plums were grown as a minor crop, using ipm practices. the shot-hole disease (wilsonomyces carpophilus) and fruit rot were monitored in the field. samples of twigs and leaves were taken for further examination if some other disease symptoms were observed ...201527141740
impact of uv-b radiation on clonostachys rosea germination and growth.sensitivity to uv-b radiation is one of the main limitations of biological control of plant pathogens in the field. the effect of uv-b radiation on germination and leaf tissue colonization by the biological control agent clonostachys rosea was evaluated. there were variations among c. rosea strains in sensitivity to uv-b radiation. the most tolerant strain (lqc62) had relative germination of about 60 % after irradiation of 4.2 kj m(-2). the deleterious effects of uv-b radiation on c. rosea colon ...201222806155
growth and asymmetry of soil microfungal colonies from "evolution canyon," lower nahal oren, mount carmel, israel.fluctuating asymmetry is a contentious indicator of stress in populations of animals and plants. nevertheless, it is a measure of developmental noise, typically obtained by measuring asymmetry across an individual organism's left-right axis of symmetry. these individual, signed asymmetries are symmetrically distributed around a mean of zero. fluctuating asymmetry, however, has rarely been studied in microorganisms, and never in fungi.201222523554
an atp-binding cassette pleiotropic drug transporter protein is required for xenobiotic tolerance and antagonism in the fungal biocontrol agent clonostachys rosea.atp-binding cassette (abc) transporters mediate active efflux of natural and synthetic toxicants and are considered to be important for drug tolerance in microorganisms. in biological control agents (bca), abc transporters can play important roles in antagonism by providing protection against toxins derived from the fungal prey and by mediating the secretion of endogenous toxins. in the present study, we generated deletion and complementation strains of the abc transporter abcg5 in the fungal bc ...201424654977
transcriptomic profiling to identify genes involved in fusarium mycotoxin deoxynivalenol and zearalenone tolerance in the mycoparasitic fungus clonostachys rosea.clonostachys rosea strain ik726 is a mycoparasitic fungus capable of controlling mycotoxin-producing fusarium species, including f. graminearum and f. culmorum, known to produce zearalenone (zea) and deoxynivalenol (don). don is a type b trichothecene known to interfere with protein synthesis in eukaryotes. zea is a estrogenic-mimicing mycotoxin that exhibits antifungal growth. c. rosea produces the enzyme zearalenone hydrolase (zhd101), which degrades zea. however, the molecular basis of resist ...201424450745
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