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[helminth species of goats in germany].the helminth fauna of the gastrointestinal tract of 25 and the respiratory organs and the livers of 6 german goats was qualitatively and quantitatively examined. one trematode species (dicrocoelium dendriticum), 2 species of cestodes (moniezia expansa and metacestodes of taenia hydatigena) and 28 species of nematodes (24 in the gastrointestinal tract and 4 in the lungs) were recorded. two goats were infested with oestrus ovis larvae. the most prevalent species were ostertagia circumcincta and ch ...19989880938
cysteine conjugate beta-lyase activity in three species of parasitic helminth.living organisms employ a variety of metabolic pathways when detoxifying xenobiotic compounds, including the formation of cysteine s-conjugates via glutathione conjugation. however, cysteine conjugate beta-lyase (ccbl) catalysed beta-cleavage, of certain cysteine conjugates, is known to cause cytotoxicity. this study represents the first investigation into the expression of ccbl and other associated enzymes in helminth species. a survey of the three major groups of parasitic helminths [cestodes ...199910428630
the neuropeptide f (npf) encoding gene from the cestode, moniezia expansa.neuropeptide f (npf) is an abundantly expressed neuropeptide in platyhelminth nervous systems, and exhibits a moderate, myogenic effect on muscle preparations of parasitic flatworms. npf displays structural similarities to peptides from molluscs and vertebrate members of the neuropeptide y (npy)-superfamily of peptides. npy is one of the most abundant and highly conserved neuropeptides within vertebrates and similarities between the gene organization of npy, pancreatic polypeptide (pp) and pepti ...200010726268
purification and characterisation of a novel cysteine conjugate beta-lyase from the tapeworm moniezia expansa.the paper presents the first report of the purification of an invertebrate cysteine conjugate beta-lyase (ccbl). ccbl activity was shown to predominate within the cytosolic fraction of tissue from the tapeworm moniezia expansa. the monomeric cytosolic enzyme was isolated with a m(r) of 72 kda and co-purified with transaminase activity towards l-aspartate. the substrate profile for m. expansa ccbl is different from that of mammalian ccbls. exploiting the differences in mammalian and parasite subs ...200010779568
oribatid mites (acari, oribatida) as intermediate hosts of tapeworms of the family anoplocephalidae (cestoda) and the transmission of moniezia expansa cysticercoids in south africa.six species of adult oribatid mites (galumna racilis, kilimabates pilosus, kilimabates sp., scheloribates fusifer, muliercula ngoyensis and zygoribatula undulata) and two immature stages belonging to the superfamilies galumnoidea and ceratozetoidea were isolated from a lawn (mixed pennisetum and cynodon spp.) at onderstepoort veterinary institute, south africa. the mites were subsequently used in an infection trial using moniezia expansa eggs. tapeworm cysticercoids were recovered in g. racilis, ...200010843322
molecular identity of major cross-reactive adult antigens in fasciola gigantica, toxocara vitulorum and moniezia expansa.cross reactivity between fasciola gigantica, toxocara vitulorum and moniezia expansa whole worm extracts was proved by elisa. intense cross-reaction was observed between f. gigantica and m. expansa rather than between each of them and t. vitulorum. as judged by immunoblot, the cross-reactive antigens in f. gigantica which recognized by t. vitulorum antisera was 109 kd while this component in addition to another one of 52 kd were detected by m. expansa sera in the same extract. furthermore, t. vi ...200010946516
structural characterization and immunolocalization of egg antigens cross-react with toxocara vitulorum, fasciola gigantica and moniezia expansa mature flukes.a structural homology between eggs of toxocara vitulorum, fasciola gigantica and moniezia expansa was proved by the use of sds-page. in immunoblot, 9, 11 and 7 polypeptides were recognized in f. gigantica, m. expansa and t. vitulorum eggs respectively by their respective rabbit anti-adult antisera. moreover, components of 240 kd and 206 kd were recognized in the three eggs by different anti-adult antisera. the anatomic localization of the crossreactive epitopes in eggs was determined by indirect ...200010946518
a confocal microscopical study of the musculature of adult schistosoma mansoni.using the filamentous actin marker, fitc-conjugated phalloidin, the major muscle systems of adult male and female schistosomes have been examined. the body wall musculature comprises an outer sheath of circular fibres, within which there is a compact layer of short, spindle-shaped longitudinal fibres and a lattice-like arrangement of inner diagonal fibres. within the oral sucker and acetabulum 3 fibre types, circular, radial and longitudinal can be distinguished. the wall of the oesophagus is li ...200011085236
comparative metabolism of albendazole and albendazole sulphoxide by different helminth parasites.albendazole (abz) is a broad-spectrum benzimidazole anthelmintic widely used in human and veterinary medicine. the aim of the current work was to characterise the sulphoxidative metabolism of abz, and the sulphoreduction of abz sulphoxide (abzso), by microsomal (ms) and cytosolic (cyt) fractions of three different helminth species: fasciola hepatica, moniezia expansa and ascaris suum. after the incubation assays, parasite material was analysed by hplc to characterise the metabolic product formed ...200111355675
parasites of domestic and wild animals in south africa. xxxix. helminth and arthropod parasites of angora goats in the southern karoo.parasites were collected from 160 angora goats and kids on the jansenville experimental farm, eastern cape province. six nematodes were identified to species level and three to generic level. nematodirus spathiger was the most numerous of the economically important nematodes recovered. it was always present, and fourth stage larvae were collected from untreated goats from august to march, while animals used as tracers also picked up most infection from august to march. kids born on the farm duri ...200111403427
the establishment, composition and severity of infection of gastro-intestinal parasites and their impact on productivity of tswana kids in southern botswana.the presence of gastro-intestinal parasites in tswana kids (n = 7) aged 1-3 weeks was studied for a period of 6 months at the botswana college of agriculture. the aims of this study were to find the time when they first contracted internal parasite infections, as well as to determine the severity of the infections and also its relation to production indicators (body mass and packed cell volume) of the kids as they grew older. the results indicate that they contracted coccidial and roundworm infe ...200111403433
epidemiological studies on gastrointestinal helminths of dromedary (camelus dromedarius) in semi-arid lands of eastern ethiopia.a total of 752 dromedaries were examined and 75% were found to be harbouring nematode eggs. the mean epg was 1831 and the range was from 100 to 21,200. the prevalence rates in the four-age groups examined were 59.6% (3-7 years), 72.4% (8-12 years), 76.1% (13-17 years) and 83.9% (18-22 years). the prevalence rate for females and males were 77.6 and 64.8%, respectively and for long dry, short rainy, short dry and long rainy seasons were 66, 80, 69 and 82.6%, respectively. the mean epg of faeces wa ...200211900928
enantioselective binding of albendazole sulphoxide to cytosolic proteins from helminth parasites.the pharmacological effect of the active albendazole metabolite, albendazole sulphoxide (abzso), depends on its sustained presence at the site of parasite location and its binding to helmith beta-tubulin. abzso is found in the plasma and tissues of albendazole-treated animals in two enantiomeric forms: (+)abzso and (-)abzso. knowledge of enantioselectivity in drug action is necessary, since any difference in target proteins affinity between enantiomers may have implications on the pharmacologica ...200211980387
the nmr-derived conformation of neuropeptide f from moniezia expansa.the solution structure of neuropeptide f (npf), from the flatworm (platyhelminthes) moniezia expansa, has been determined by (1)h nmr spectroscopy at 800 mhz in 60%/40% cd(3)oh/h(2)o. npf is the most abundant neuropeptide in platyhelminthes. the secondary structure of npf contains an alpha helix from residues lys(14) to ile(31), while the n terminus, consisting of residues pro(-2) to asn(13), and the c-terminus, consisting of residues gly(32) to phe(36), are in a random conformation. the structu ...200212023801
the endoparasites of sheep and goats, and shepherd in north sinai governorate, egypt.examination of sheep and goats in four areas in north sinai revealed an overall infection of 12.70% with fasciola species, 11.8% with paramphistomum cerve, 12.80% with moniezia expansa, 47.11% with trichostrongylus colubriformis, 4.59% trichuris ovis and 44.85% coccidia. the highly infected sheep and goats were diagnosed in el hassanah center (90.49%), followed by al arish city (87.31%), then rafah city (74.39%), and lastly bir al abd (54.71%). the elevation of the overall percent of infection i ...200212049248
gene organization and expression of a neuropeptide y homolog from the land planarian arthurdendyus triangulatus.neuropeptide y is one of the most widespread regulatory peptides within the vertebrate nervous system and shares the c-terminal motif [fy]-x(3)-[livm]-x(2)-y-x(3)-[livmfy]-x-r-x-r-[yf] with pancreatic polypeptide, peptide yy, and fish pancreatic peptide y. all four peptides are believed to have arisen from a single ancestral gene through successive gene duplication events in vertebrates. the origin of this peptide family may date back further still; similarly sized peptide transmitters with an i ...200212410618
efficacy of praziquantel (cesocide injection) in treatment of cestode infections in domestic and laboratory animals.efficacy of praziquantel (cesocide injection) by intramuscular (i.m.) route against cestode infections was evaluated. total 93 domestic or laboratory animals such as dogs, cats, rats, mice, goats, deers and chickens were used. animals were infected with dipylidium caninum, spirometra sp., taenia pisiformis, taenia taeniaeformis, hymenolepis nana, moniezia expansa, moniezia sp. or raillietina sp. a single dose of praziquantel, 6 mg/kg of body weight, was highly effective (97.9%) against cestodes ...198812811058
[metabolism of c(14)-glucose by moniezia expansa and diphyllobothrium mansoni]the adult worms of cestodes, moniezia expansa and diphyllobothrium mansoni employed in this experiment. the worms were divided into three portions, i.e. immature, mature and gravid proglottids, and each proglottids were incubated in a certain incubation period, and the glucose uptake rate, total co2 production rate, tissue concentration and their radioactivities were employed as previous reports(rim et al., 1965). 1. the glucose uptake rate by m. expansa was a mean value of 6.46 +/- 1.23 micro m ...196512913582
[metabolism of c(14)-acetate by cestodes]the adult worm and plerocercoid larva(sparganum) of diphyllobothrium mansoni and moniezia expansa employed in this experiment. the adult worms were divided into three portions, i.e. immature, mature and gravid proglottids, and each proglottids were incubated in 50 cc or 250 cc volume of special incubation flasks with incubation medium consisting of 10 cc of 25 cc of krebs-ringer phosphate buffer (ph 7.4). the incubation medium was added c(14)-acetate and non-radioactive carrier na-acetate so as ...196512913584
[hatching and activation of some cestode ova: the effects of various artificial hatching-activating solutions upon the some cestode ova]the effects of various digestive enzymes, fresh biles, and bile acids on the hatching and activation of the eggs of taenia saginata, t. pisiformis, t. solium, hymenolepis nana, and moniezia expansa have been investigated. on the basis of the results of the present study author attempted to elucidate the specificity of host-parasite relationships in the tapeworm infections. the results were summarized as follows: 1)in case of the ova of t. saginata, pretreatment with artificial gastric juice befo ...197112913619
studies on phosphatase systems of cestodes. ii. studies on cysticercus tenuicollis and moniezia expansa (adult). 195713441308
nitrogen and amino acid composition of three species of anoplocephalid cestodes: moniezia expansa, thysanosoma actinioides, and cittotaenia perplexa. 196013807281
[llama huanachus molina and llama glama linneus, new hosts of moniezia expansa (rud., 1810) (cestoda, anoplocephalidae)]. 196314060697
internal parasites of free-ranging guanacos from patagonia.in the winter of 2000, a greater than 80% reduction in the guanaco population located in cabo dos bahi;as wildlife reserve, chubut, argentina, was evident due to massive mortality attributed to starvation. twelve guanacos were necropsied and samples were analyzed at the parasitology laboratory of facultad de ciencias veterinarias, universidad nacional del litoral. fecal analysis revealed developmental stages of nematodirus sp., marshallagia sp., trichuris sp. and eimeria spp. histopathological a ...200314651877
on the finding of an intermediary host for moniezia expansa (rud. 1810) in eastern quebec. 195114801733
local and systemic immune responses to echinococcus granulosus in experimentally infected dogs.local and systemic immune responses were studied in six dogs experimentally infected with the dog/sheep tapeworm echinococcus granulosus. all dogs developed similar igg antibody response to parasite antigens. in contrast, ige and iga responses differed widely. no relationship between iga responses and parasite burden at the end of the infection were observed. further, clear differences in the anti-parasite iga response in serum as compared with specific iga forming cells in mesenteric lymph node ...200415036575
laboratory and field studies on oribatid mites as intermediate host of moniezia expansa infecting egytptian sheep.three species of oribatid mites, scheloribates zaherii, zygoribatula tadrosi and z. sayedi from pure colonies were experimentally exposed to infection by allowing them to feed on stool sheep infected with moniezia expansa. the mites were followed up to the development of the infective cysticercoids. m. expansa was able to achieve sucessfully its larval development in the three species of oribatid mites under laboratory conditions. these were demonstrated after 84, 73 & 69 days post infection, re ...200415125535
human monieziasis expansa: the first egyptian parastic zoonosis.this paper reports the first egyptian zoonotic infection with the common tapeworm of sheep, moniezia expansa. perhaps this is the first human monieziasis expansa in the sheep raising countries worldwide. diagnosis was based on recovery of the characteristic eggs from the stool of a fifteen-years-old shepherd. a single dose of niclosamide one gram proved effective.200415287174
efficacy of commiphora molmol (mirazid) against sheep naturally infected with monieziasis expansa in al-santa center, gharbia governorate, egypt.the efficacy of commiphora molmol or myrrh (mirazid) was evaluated in treating sheep naturally infected with moniezia expansa. total doses of one, two or three capsules (300 mg each) were given for one, two, three, four, five, six, seven and eight successive days on an empty stomach an hour before offering their breakfast. every day the stools of the three groups were examined microscopically and macroscopically for eggs and/or gravid segments. when the stool was negative, the treatment was stop ...200415587306
moniezia expansa in farmed red deer (cervus elaphus). 198316030941
tapeworm (moniezia expansa) in sheep: anthelmintic treatment studies to assess possible pathogenic effects and production loss in young infected animals in the field.niclosamide and cupric acetoarsenite were both effective in removing tapeworm (moniezia expansa) from unweaned lambs. in four trials there was no significant liveweight gain in niclosamide-drenched, compared with undrenched, animals. post-mortem examination for tapeworm showed that although one lamb contained 200 ml (bulk volume) of strobila, 50% of 49 infected animals contained 50 ml or less. the greatest number of scoleces in one animal was 41, and 65% of lambs contained four or fewer. m.expan ...198416031018
tapeworm (moniezia expansa) and its effect on sheep production: the evidence reviewed.literature relating to the effects of the tapeworm moniezia expansa on sheep is reviewed. while there are many claims of adverse effects by this organism, none of these withstand critical scrutiny. the balance of evidence indicates that m. expansa infections are generally harmless, even when the tapeworms are present in large numbers in young lambs, and that there is no justification for treating sheep for m. expansa on the basis of any likely benefit to the health or production of the animals. ...198616031272
use of praziquantel for the control of moniezia expansa in lambs.the efficacy of a drench containing praziquantel in combination with levamisole was evaluated in four trials performed in the 1990-91 and 1992-93 seasons in the waikato region of new zealand. the trials involved 93 naturally infected lambs and compared the efficacy of a 3.75 mg/kg praziquantel 7.5mg/kg levamisole drench against moniezia expansa, with albendazole, an albendazole-levamisole combination and oxyclozanide-levamisole combination in controlled trials. there was no significant reduction ...199616031907
mixed infection with intestinal tape worms in sheep.in a survey carried out during the period april 2001 to november 2002, the intestines of 3165 slaughtered sheep in the fars province of iran were examined for mixed infection with intestinal tape worms. four point sixty eight percent of animals were found to be infected with one or more than one species of tape worms. the identified species included: moniezia expansa, moniezia benedeni, thysaniezia giardi, avitellina centripunctata and stilesia globipunctata. of all 148 infected sheep, 104 anima ...200416493395
influence of protein supplementation on the resistance and resilience on young hair sheep naturally infected with gastrointestinal nematodes during rainy and dry seasons.thirty, 4-month-old entire santa ines lambs were grazed on an andropogon gayanus pasture, during a 34-week period (rainy season weeks 0-20 and dry season weeks 21-34) and allocated in two treatment groups (n = 15) each with different protein supplementation: high protein (hp-19% cp) and low protein (lp-11% cp). these were subdivided into those receiving anthelmintic treatment (c) (n = 7) and without anthelmintic treatment (i) (n = 8). the objective was to evaluate the effects of supplementation ...200616495016
effects of condensed tannin from acacia mearnsii on sheep infected naturally with gastrointestinal helminthes.the effect of tannins on endoparasite control in hair sheep was investigated using 20 entire lambs of the santa inês breed. at the beginning of the experiment these animals were 6-months old and weighed 22.5kg+/-4.7. the treatments used were (10 animals each): gt (animals receiving 18g of acácia negra containing 18% of condensed tannin/animal/week) and gc (animals not receiving tannin). the experiment lasted 84 days, with animals kept on an andropogon gayanus pasture. faeces were collected weekl ...200717067741
genetic markers to distinguish moniezia expansa from m. benedeni (cestoda: anoplocephalidae) and evidence of the existence of cryptic species in australia.multilocus enzyme electrophoresis (mee) was used to genetically compare moniezia spp. collected from sheep and cattle in southern australia. fifteen enzyme loci were established for the genetic characterisation of individual specimens of moniezia, which represents a significant increase in the number of loci that was previously available for these cestodes. another four enzyme loci were also identified as providing potential genetic markers. there were no differences in staining activity or band ...200717206509
the life cycle of moniezia expansa. 193717794455
effects of neuropeptide f on regeneration in girardia tigrina (platyhelminthes).the effects of neuropeptide f (npf; from moniezia expansa) on the regeneration of girardia tigrina were studied. the animals were decapitated and incubated in water (control) or npf. the dynamics of the proliferation of the neoblasts in the developing tissue were studied during the course of regeneration by monitoring the mitotic index (mi). the effects of incubation in fmrfamide and gyirfamide on the mi were also tested. the course of cephalic regeneration was followed with in vivo computer-ass ...200818095002
prevalence and seasonal incidence of larval and adult cestode infections of sheep and goats in eastern ethiopia.a study on the prevalence and seasonal incidence of cestode parasite infections of sheep and goats was carried out in eastern ethiopia for 2 years (may 2003-april 2005). during this period, viscera including liver, lungs, heart, kidneys and the gastro-intestinal tract were collected from 655 sheep and 632 goats slaughtered at four abattoirs located in the towns of haramaya, harar, dire dawa and jijiga. at the abattoirs the abdominal, thoracic and pelvic cavities as well as the muscle surfaces of ...200818575964
occurrence of moniezia expansa (rud, 1810) blanchard, 1891 (cestoda: anoplocephalidae) in domestic pig (sus scrofa domestica linnaeus, 1758) in perú.the presence of moniezia expansa (rud, 1810) blanchard, 1891, is reported in domestic pig (sus scrofa domestica linnaeus, 1758). four tapeworms were collected and identified as m. expansa. this is the first report of m. expansa collected in a domestic pig in perú.200819028016
generation and analysis of expressed sequence tags from a cdna library of moniezia expansa.the moniezia expansa is a parasite of sheep that causes diarrhea and fleshless leading to stockbreeding losses. a genomic resource for large-scale molecular studies in this parasite is lacking. to study the gene expression including development, digestion and reproduction organs of m. expansa, a cdna library had been constructed and expressed sequence tags (ests) had been analyzed, which were helpful for the development of a powerful microarray platform which are used to analyze gene expression ...200919118581
prevalence of cestode parasites in sheep slaughtered in riyadh city, saudi arabia.prevalence of tapeworm infections among sheep slaughtered in riyadh city were studied from february 2007 to march 2008. a total of 76 out of 3300 sheep were infected with adult cestodes (2.3%). the highest infection rate was in autumn (8.1%), and the lowest one was in summer (1.7%). the cestodes were identified as moniezia expansa (96.3%) and m. deuticulata (3.7%). concerning seasonal dynamics of the worms, the highest number was recorded in october and november (13.5 & 8.3%), while the lowest o ...200819143137
characterization of a moniezia expansa ubiquitin-conjugating enzyme e2 cdna.ubiquitin and other ubiquitin-like proteins play important roles in post-translational modification. they are phylogenetically well-conserved in eukaryotes. here we report a new ubiquitin-conjugating enzyme e2 cdna, which containing a ubiquitin-conjugating enzyme ubcc-domain named ube2am. its cdna is 899 base pairs in length and contains an open reading frame from nucleotide 171 to 632 encoding 153 amino acids. the result of real time rt-pcr showed that ubea2 m is expressed in most of m. expansa ...201019468864
genetic factors of sheep affecting gastrointestinal parasite infections in the distrito federal, brazil.three sheep farms were used in the distrito federal, central brazil, to study the occurrence of parasites in the feces. a total of 1798 collections were taken over the period of a year. a total of 1205 were taken in santa inês breed (si) in all three farms, 323 in bergamasca (berg), 54 in ile de france, 49 in ilexsi, 103 in morada nova (mn) and 64 in texelxsi, these last five groups being on a single farm. the animals were drenched soon after weaning and feces collected every 3 weeks to calculat ...200919837513
rna interference in a cestode reveals specific silencing of selected highly expressed gene transcripts.evolving rna interference (rnai) platforms are providing opportunities to probe gene function in parasitic helminths using reverse genetics. although relatively robust methods for the application of rnai in parasitic flatworms have been established, reports of successful rnai are confined to three genera and there are no known reports of the application of rnai to the class cestoda. here we report the successful application of rnai to a cestode. our target species was the common ruminant tapewor ...201019900457
experimental studies on the lead accumulation in the cestode moniezia expansa (cestoda: anoplocephalidae) and its final host (ovis aries).the tapeworm moniezia expansa and naturally infected sheep were investigated with respect to their lead accumulation. lead-pb(ch(3)coo)(2) was added to the distilled water and administered orally to the sheep every day for a period of 1 week. after the exposure period the sheep were killed and the metal levels were determined in the muscle, liver, kidney and blood of the sheep as well as in the cestode parasites (moniezia expansa). the impact of an infection with the cestode moniezia expansa and ...201020213435
a new intermediate host (protoschelobates seghettii, n. sp.: acarina: scheloribatidae) of the sheep tapeworm, moniezia expansa. 194720262495
observations on oribatid mites, vectors on moniezia expansa on pastures, with a report of several new vectors from the u.s. 194720340873
experimental studies on the cadmium accumulation in the cestode moniezia expansa (cestoda: anoplocephalidae) and its final host (ovis aries).the tapeworm moniezia expansa and naturally infected sheep were investigated with respect to their cadmium accumulation. cadmium chloride (cdcl(2), 0.2 g) was added to 10 ml of distilled water and administered orally to the sheep every day for a period of 1 week. the cadmium content of m. expansa was lower than that in the liver tissues of sheep, although this difference was not significant. the highest mean cadmium concentrations were found in the liver of sheep infected with m. expansa (24.5+/ ...201020435007
studies on monieziasis of sheep i. prevalence and antihelminthic effects of some plant extracts, a light and electron microscopic study.out of 2,343 examined sheep throughout the whole year, 271 were found to be infected with tapeworms (11.5%). the highest infection rate (22.1%) was recorded in autumn, while the lowest (6%) occurred in summer. six species of cestodes were identified according to their prevalence: moniezia expansa (74%), moniezia denticulata (8.5%), moniezia benedeni (4.8%), moniezia trigonophora (2.7%), and thysaniezia giardi (2.7%), t. giardi was recorded in egypt for the first time. m. expansa was found throug ...201020865430
a preliminary report on use of lead arsenate for removal of the sheep tapeworm, moniezia expansa. 194620988122
calibration and diagnostic accuracy of simple flotation, mcmaster and flotac for parasite egg counts in sheep.the present study was aimed at carrying out a calibration and a comparison of diagnostic accuracy of three faecal egg counts (fec) techniques, simple flotation, mcmaster and flotac, in order to find the best flotation solution (fs) for dicrocoelium dendriticum, moniezia expansa and gastrointestinal (gi) strongyle eggs, and to evaluate the influence of faecal preservation methods combined with fs on egg counts. simple flotation failed to give satisfactory results with any samples. overall, flotac ...201021216533
parasites of chamois in new zealand.abstract sir, - andrews'((2)) checklist of helminth parasites of wild ruminants in new zealand recorded the presence of 10 species of gastrointestinal nematodes in chamois (rupicapra rupicapra rupicapra l.) from "central south island". in october 1978 we examined 28 freshly-shot chamois cadavers from the harper-avoca watershed in the headwaters of the rakaia river. six of the nematode species reported by andrews were found again; viz. ostertagia ostertagi, o. circumcincta, o. trifurcata, nemat ...198122133071
gene expression profiling of in moniezia expansa at different developmental proglottids using cdna microarray.gene expression profiles of moniezia expansa proglottids at varying developmental stages were analysed using cdna microarray. a total of 4,056 spots, including full length and partial complementary dnas that represent novel, known, and control genes, were studied. results indicated an up-regulation of 55 genes in immature proglottids, 134 genes in mature proglottids and 103 genes in gravid proglottids were up-regulated, and a down-regulation of 7 genes in immature proglottids, 68 genes in mature ...201122002511
gastrointestinal parasitic infection in diverse species of domestic ruminants inhabiting tribal rural areas of southern rajasthan, india.a total of 415 adult domesticated ruminants, 130 cattle (bos taurus), 108 buffaloes (bubalus bubalis), 94 goats (capra hircus) and 83 sheep (ovis aries) inhabiting tribal rural areas of southern rajasthan, india were investigated for evidence of gastrointestinal protozoan and helminthic infections. in southern rajasthan humid ecosystem is predominant and has number of perennial freshwater bodies. fresh faecal samples of these animals were examined microscopically by direct wet smear with saline ...201224431582
prevalence and scanning electron microscopic identification of anoplocephalid cestodes among small ruminants in senegal.this study was undertaken to determine the prevalence of anoplocephalid cestodes in sheep and goats in senegal. intestines of 462 sheep and 48 goats were examined; 47.4% of sheep and 6.2% of goats were infected. the species identified and their prevalence were, among sheep, avitellina centripunctata 38.7%, moniezia expansa 15.4%, stilesia globipunctata 16.7%, and thysaniezia ovilla 0.4%. among goats, they were m. expansa 6.2% and t. ovilla 2.1%. the prevalence of all species was not statisticall ...201627597893
sensitivity and efficiency of selected coproscopical methods-sedimentation, combined zinc sulfate sedimentation-flotation, and mcmaster method.coproscopical methods used in veterinary-parasitological diagnostics were validated according to their sensitivity (se) and egg recovery rate [efficiency (ef)]. validation of the combined sedimentation-flotation method and the modified mcmaster method was performed by using feces spiked with eggs of ancylostoma caninum, uncinaria stenocephala, cooperia oncophora, cyathostomins, ascaris suum, toxascaris leonina, toxocara canis, trichuris vulpis, moniezia expansa, and anoplocephala perfoliata. for ...201626997342
gastrointestinal parasites of sheep, municipality of lajes, rio grande do norte, brazil.this study investigated the gastrointestinal parasitism by helminths and protozoa in sheep (ovis aries) santa inês breed, municipality of lajes, rio grande do norte. monthly, from april 2005 to august 2007, stool samples were collected from two tracer lambs in the first day of the experiment and performed a necropsy of these animals in 44th day. a total of 64 lambs were sampled, but only 62 lambs were slaughtered. the fecal samples were examined by sedimentation in water. the contents of the abo ...201222534950
prevalence of pasture-associated metazoal endoparasites in bavarian dairy goat herds and farmers' approaches to parasite control.the majority of dairy goat farms in bavaria operate pasture-based systems. endoparasites are therefore a common problem affecting health and productivity of these herds. pooled faecal samples from 37 commercial dairy goat farms in bavaria were examined by modified mcmaster, flotation, sedimentation and baermann funnel techniques. in addition, a questionnaire was used to gather information on farmers' perceptions and parasite management efforts. the average trichostrongyle faecal egg count across ...201627529995
moniezia benedeni and moniezia expansa are distinct cestode species based on complete mitochondrial genomes.moniezia spp. parasitize the intestines of ruminants, causing monieziasis. in this study, the complete mitochondrial (mt) genomes of m. benedeni and m. expansa have been determined, characterized and employed to test the hypothesis that m. benedeni and m. expansa are distinct species by phylogenetic analysis based on the concatenated amino acid sequences derived from 12 protein-coding genes, inferred with bayesian and maximum-likelihood methods. the complete mt genomes of m. benedeni and m. expa ...201727923556
staining of platyhelminthes by herbal dyes: an eco-friendly technique for the taxonomist.an environment compatible technique to stain platyhelminthes, fasciola gigantica, gastrothylax crumenifer, taenia solium, and moniezia expansa using aqueous and alcoholic extract of sugar beet (beta vulgaris), china rose (hibiscus rosa-sinensis), and red rose (rosa hybrida) were described to minimized the deleterious effects of the synthetic dyes.201527047037
influence of massive and long distance migration on parasite epidemiology: lessons from the great wildebeest migration.very little is known about the influence of massive and long distance migration on parasite epidemiology. migration can simultaneously minimize exposure to common parasites in their habitats and increase exposure to novel pathogens from new environments and habitats encountered during migration, while physiological stress during long distance movement can lead to immune suppression, which makes migrants vulnerable to parasites. in this paper, we investigated the diversity, prevalence, parasite l ...201627554373
characterization of hydrophobic-ligand-binding proteins of taenia solium that are expressed specifically in the adult stage.taenia solium, a causative agent of taeniasis and cysticercosis, has evolved a repertoire of lipid uptake mechanisms. proteome analysis of t. solium excretory-secretory products (tsesp) identified 10 kda proteins displaying significant sequence identity with cestode hydrophobic-ligand-binding-proteins (hlbps). two distinct 362- and 352-bp-long cdnas encoding 264- and 258-bp-long open reading frames (87 and 85 amino acid polypeptides) were isolated by mining the t. solium expressed sequence tags ...201222657393
genetic characterization of moniezia species in senegal and ethiopia.genetic diversity of moniezia spp. from domestic ruminants in senegal and ethiopia was investigated based on the nucleotide sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear small subunit ribosomal rna gene (ssu rdna). a total of 64 adult tapeworms were collected from sheep, goat and cattle, and the tapeworms from cattle were all morphologically identified as moniezia benedeni. on the other hand, the tapeworms obtained from sheep and goat were identified as moniezia ex ...201525752566
[mrna expression analysis of 10 reproduction-related genes in different developmental proglottids of moniezia expansa].according to the results of transcriptome sequencing in moniezia expansa, 10 functional genes which represent different expression patterns in different developmental proglottids were selected, including kifc1, kif17, tgf-beta, smadd, tgf-beta receptor, hsd5, aminopeptidase puromycin, methionine aminopeptidase 2, transcription factor fork head and sox transcription factor. a real-time fluorescent quantitative pcr assay was developed for detection of mrna expression level of these taget genes wit ...201526080534
biotransformation of anthelmintics and the activity of drug-metabolizing enzymes in the tapeworm moniezia expansa.the sheep tapeworm moniezia expansa is very common parasite, which affects ruminants such as sheep, goats as well as other species. the benzimidazole anthelmintics albendazole (abz), flubendazole (flu) and mebendazole (mbz) are often used to treat the infection. the drug-metabolizing enzymes of helminths may alter the potency of anthelmintic treatment. the aim of our study was to assess the activity of the main drug-metabolizing enzymes and evaluate the metabolism of selected anthelmintics (abz, ...201525373326
prevalence of gastro-intestinal parasites in buffalo calves from different agro-climatic zones of punjab.faecal samples from 1,582 buffalo calves of up to 6 months of age from 13 districts falling under four major agro-climatic zones of punjab state, india were examined for gastrointestinal (gi) parasites for a period of one year (may 2008 to april 2009). the results revealed prevalence of gi parasitic infections as 73.58 per cent calves and eimeria sp. (54.55 %) was the most prevalent gi parasite. significant differences (p < 0.01) were found in overall prevalence of toxocara vitulorum, strongyles ...201425320484
sequence differences in the internal transcribed spacer 1 and 5.8s ribosomal rna among three moniezia species isolated from ruminants in japan.this study was designed to clarify the differences in the internal transcribed spacer (its) 1 and 5.8s nucleotide sequences of moniezia expansa, m. benedeni and m. monardi isolated from ruminants in japan and to determine their phylogenetic relationships. a 98% similarity in the 5.8s sequences was observed among the 3 moniezia species, whereas many nucleotide indels and substitutions were observed in the its1 sequences among the three moniezia species. these results suggest that the its1 region ...201525283945
[mrna transcription of translation elongation factor and primase in different segments of moniezia expansa].a sybr green real-time fluorescent quantitative pcr assay was developed for detection of moniezia expansa mrna with its beta-tubulin as an internal control. the results showed a good linear relationship (>0.99) between the ct value and the concentration of positive plasmid for each gene from scolex and various proglottids. real-time pcr showed that the expression abundance of translation elongation factor and primase was different. in conclusion, the transcription level of translation elongation ...201223072148
effect of lead in water on the absorption of copper, iron, manganese and zinc by sheep (ovis aries) infected with sheep tapeworm (moniezia expansa).the sheep tapeworm (moniezia expansa) and its host ovis aries were analyzed by inductively coupled plasma optical emission spectrometry (icp-oes) for their copper, iron, manganese, zinc and lead levels. element concentrations in cestode parasites were compared to those in various organs (liver, kidney, and muscle) of sheep. tapeworms in the small intestine of sheep that were administered 2g of pb(ch(3)coo)(2) per os daily (7 days) had significantly higher lead concentrations than sheep tissues. ...201222425750
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