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host marking pheromone (hmp) in the mexican fruit fly anastrepha ludens.host marking pheromones (hmps) are used by insects to mark hosts (usually a fruit) where they have already laid eggs. the compounds serve as a deterrent to conspecifics avoiding over-infestation of hosts (i.e. repeated egg-laying into an already occupied/used host). if these hmps are sprayed onto commercially valuable fruit they act as deterrents preventing attack by females interested in laying eggs into the valuable commodity. having no insecticidal or toxic properties, and being natural produ ...201021137682
chilled packing systems for fruit flies (diptera: tephritidae) in the sterile insect technique.we evaluated three packing systems (parc boxes, "gt" screen towers and "mx" screen towers) for the emergence and sexual maturation of sterile fruit flies, at three adult fly densities (1, 1.2 and 1.3 fly/cm²) and three food types. at the lowest density, results showed no significant differences in the longevity and flight ability of adult anastrepha ludens (loew) and anastrepha obliqua macquart among the three packing systems. higher densities resulted in a decrease in these parameters. in the e ...201020877998
ionizing radiation as a phytosanitary treatment against fruit flies (diptera: tephritidae): efficacy in naturally versus artificially infested fruit.some phytosanitary irradiation treatment research against tephritid fruit flies (diptera: tephritidae) has used artificially infested fruit with the unstated and untested assumption that the method adequately simulated a natural situation. we compare grapefruit, citrus paradisi macfayden, naturally infested by mexican fruit fly, anastrepha ludens (loew), via oviposition until larvae reached the late third instar versus insertion of diet-reared third instars into holes made in grapefruits 24 h be ...201020857719
reproductive aging in tephritid fruit flies.the broad objective of this paper is to present an overview and synthesis of selected studies on reproduction and aging in two model tephritid fruit fly species including the mediterranean fruit fly, ceratitis capitata, and the mexican fruit fly, anastrepha ludens. we summarize the research findings from empirical studies and modeling investigations involving reproduction in the two tephritid species. at the end we identify and discuss four general principles regarding reproductive aging in teph ...201020738284
development of transgenic strains for the biological control of the mexican fruit fly, anastrepha ludens.the mexican fruit fly, anastrepha ludens, is a highly significant agricultural pest species that has been genetically transformed with a piggybac-based transposon vector system using independent vector and transposase helper plasmids. minimum estimated germ-line transformation frequencies were approximately 13-21% per fertile g(0) individual, similar to previously reported frequencies using single vector-helper plasmids. two vector constructs were tested with potential importance to transgenic s ...201120737195
resistance of mexican fruit fly to quarantine treatments of high hydrostatic pressure combined with heat.high-pressure processing (hpp) has been proposed as an alternative quarantine method against the mexican fruit fly anastrepha ludens loew (diptera: tephritidae), which is one of the most important pests infesting mangoes, citrus, and other fruits in mexico and other latin-american countries. however, processing conditions used to destroy eggs and larvae also affect the shelf life of fruits. the objective of this study was to assess the biological viability of a. ludens eggs treated with hpp, est ...201020367391
efficacy of delayed atmospheric modification in a heat/modified atmosphere phytosanitary treatment.the combination of heat and low levels of oxygen increases mortality to insects infesting fruit compared with either heat or low oxygen alone. this combination treatment shows promise to disinfest commodities of quarantine pests. heated air/modified atmosphere treatments employ the modified atmosphere (e.g., low oxygen) during the entire treatment interval. there is a positive relationship between temperature and efficacy of heat/modified atmosphere treatments. efficacy of delaying atmospheric m ...201020214365
an improved quarantine method for mangoes against the mexican fruit fly based on high-pressure processing combined with heat.the mexican fruit fly anastrepha ludens loew (diptera: tephritidae) is one of the most important insects infesting mangoes, citrus, and other fruits in mexico and other latin-american countries. quarantine methods approved to destroy this insect decrease the shelf life of commodities. the objective of this study was to determine the effect of high-pressure processing using an initial temperature of 50 degrees c on the survivorship of eggs and larvae of the mexican fruit fly. eggs and larvae were ...201020001324
high hydrostatic pressure at low temperature as a quarantine treatment to improve the quality of fruits.application of high hydrostatic pressure has been proposed as an alternative quarantine process for the mexican fruit fly, anastrepha ludens loew (diptera: tephritidae), one of the most important insects infesting mangoes, citrus fruits, and other fruits in mexico and other latin american countries. the present study was performed to determine the effect of high pressure treatments at 0 degrees c on the survivorship of eggs and larvae of this pest. the effect of time and pressure level at near-f ...201019899960
male and female condition influence mating performance and sexual receptivity in two tropical fruit flies (diptera: tephritidae) with contrasting life histories.recent recognition of widespread polyandry in insects has generated considerable interest in understanding why females mate multiple times and in identifying factors that affect mating rate and inhibit female remating. however, little attention has been paid to understanding the question from both a female and male perspective, particularly with respect to factors that may simultaneously influence female remating rates. here, we report on a study aimed at ascertaining the possible interactive ef ...200919666025
leg impairments elicit graded and sex-specific demographic responses in the tephritid fruit fly anastrepha ludens.this study was concerned with the impact of different levels of artificial impairment (leg amputations) on male and female survival and female reproduction in the mexican fruit fly, anastrepha ludens. we monitored the demographic responses in a total of 100 flies of each sex that were maintained individually in 4x4x10 cm cages and subject to 1-of-11 different leg amputations (plus intact control) including cohorts in which either one front, one middle or one rear leg was severed (3 cohorts total ...200919457447
mitotic and polytene chromosome analysis in the mexican fruit fly, anastrepha ludens (loew) (diptera: tephritidae).the present study constitutes the first attempt to construct a polytene chromosome map of an anastrepha species, anastrepha ludens (loew), a major agricultural pest. the mitotic karyotype has a diploid complement of 12 acrocentric chromosomes, including five pairs of autosomes and an xx/xy sex chromosome pair. the analysis of salivary gland polytene chromosomes has shown a total number of five polytene elements that correspond to the five autosomes. the characteristic features and the most promi ...200919132068
germ-line transformation of the mexican fruit fly.germ-line transformation of a major agricultural pest, the mexican fruit fly (anastrepha ludens loew, mexfly), was achieved using composite piggybac transposable elements marked with green, yellow and red fluorescent proteins (copgreen, phiyfp and j-red). we also investigated the possibility of generating transposon-free insertions, in order to address potential concerns relating to proposed field use of transgenic mexfly. we describe a highly efficient method for transforming mexfly, compare ef ...200717894556
colonization of a hybrid strain to restore male anastrepha ludens (diptera: tephritidae) mating competitiveness for sterile insect technique programs.to restore male mating competitiveness of mexican fruit flies, anastrepha ludens (loew) (diptera: tephritidae), reared for sterile insect releases by the mexican fruit fly eradication campaign, two strain replacement techniques were evaluated. field cage male competitiveness tests revealed that laboratory males of the metapa strain mated 3 times less often with wild females than field-collected wild males. a strain developed from the cross of wild males and laboratory females (hybrid strain) was ...200717598535
cryopreservation of mexican fruit flies by vitrification: stage selection and avoidance of thermal stress.this report presents details of a vitrification methodology for the cryopreservation of embryos of the mexican fruit fly, anastrepha ludens. the overall summary of the data indicates that selecting the correct developmental stage for cryopreservation is the most important criterion. the key aspect in selection of the correct stage is to balance depletion of the gut yolk content against development of the embryonic cuticle. embryogenesis was divided into four stages between 90 and 120 h after inc ...200717150205
nontarget impact of spinosad gf-120 bait sprays for control of the mexican fruit fly (diptera: tephritidae) in texas citrus.bait sprays containing the toxicant spinosad (gf-120) were applied to citrus groves in the rio grande valley of texas where mexican fruit flies were detected in surveillance traps. the sprays were applied as a supplement to a continuous sterile insect release program. sterile fly captures were 47-63% lower in the treated groves compared with control groves. eight of 10 secondary pest populations declined in the test groves subsequent to spray applications, but they also declined in the control g ...200516539119
pupariation time as a source of variability in mating performance in mass-reared anastrepha ludens (diptera: tephritidae).the effect of time of pupariation on pupal weight and adult sexual competitiveness under field cage conditions was studied in mass-reared anastrepha ludens (loew) males. larvae that took 72 h to pupariate after separation from diet resulted in lighter pupae than those that took 24 and 48 h. wild pupae were heavier than the 48- and the 72-h pupae but not the 24-h pupae. interestingly, no differences in mating performance were found between males of the 24- and 48-h pupae despite differences in pu ...200516539116
reaction orders for thermal mortality of third instars of mexican fruit fly (diptera: tephritidae).mexican fruit fly, anastrepha ludens (loew), is a quarantine pest of several fruit, including citrus, avocados, and mangoes, from extreme southern texas to costa rica. to provide information for modeling heat phytosanitary treatments, third instars were heated with an aluminum heating block between 44 and 50 degrees c for time intervals up to those causing 100% mortality. at 44 and 50 degrees c, 100% mortality was achieved at 100 and 2 min, respectively. each 2 degrees c increase in temperature ...200516539112
efficacy of two synthetic food-odor lures for mexican fruit flies (diptera: tephritidae) is determined by trap type.sterile mass-reared mexican fruit flies, anastrepha ludens (loew), were trapped in a citrus orchard by using multilure traps and cylindrical sticky traps baited with advanced pheromone technologies anastrepha fruit fly (aff) lures or suterra biolure two-component (ammonium acetate and putrescine) mff lures (biolures). the cylinder trap/aff lure combination was the best trap over the first 6 wk, the multilure trap/biolure combination was best during weeks 6-12, and the multilure trap/aff lure com ...200516334319
mass rearing history negatively affects mating success of male anastrepha ludens (diptera: tephritidae) reared for sterile insect technique programs.mating competitiveness and sterility induction into cohorts of wild anastrepha ludens (loew) (diptera: tephritidae) was compared among wild and laboratory flies reared for use in the sterile insect technique mexican program. laboratory flies stemming from an 11-yr-old bisexual strain were either not irradiated, irradiated at 3 krad (low dose), or irradiated at 8 krad. in 30 by 30 by 30-cm plexiglas cages, where a cohort of laboratory flies (male and female) irradiated at different doses (0, 3, a ...200516334318
grapefruit oil enhances attraction of mexican fruit flies to a synthetic food-odor lure.we investigated the attractiveness of grapefruit oil to the mexican fruit fly. only high concentrations were attractive in laboratory wind-tunnel bioassays. attraction of flies to grapefruit oil was not enhanced if they had previous experience with grapefruit. in citrus orchard experiments, undiluted grapefruit oil attracted mexican fruit flies and enhanced attraction to traps baited with a synthetic food-odor lure emitting ammonia and other nitrogenous chemicals. this is the first demonstration ...200516124231
efficacy of 1,4-diaminobutane (putrescine) in a food-based synthetic attractant for capture of mediterranean and mexican fruit flies (diptera: tephritidae).field trials were conducted in guatemala to evaluate the importance of 1,4 diaminobutane (putrescine) in traps baited with ammonium acetate, trimethylamine, and putrescine. for the mediterranean fruit fly, ceratitis capitata (wiedemann), there were no differences in percentage of females captured in coffee and citrus or in percentage of males captured in citrus in traps with ammonium acetate and trimethylamine lures (females in coffee, 26.4 +/- 6.27%; females in citrus, 35.7 +/- 5.35%; males in ...200415279300
irradiation of mangoes as a postharvest quarantine treatment for fruit flies (diptera: tephritidae).mangoes infested with third instar larvae were irradiated using co-60 gamma rays and a dose interval of 2-250 gy to assess the irradiation dose required to prevent adult emergence of the mexican fruit fly (anastrepha ludens), the west indies fruit fly (a. obliqua), the sapote fruit fly (a. serpentina), and the mediterranean fruit fly (ceratitis capitata). doses of 76.9, 87.3, 91.4 and 112.7 gy, were estimated to inhibit 99.9968% (probit 9) of adult emergence fora. obliqua, a. serpentina, a. lude ...200415154447
age estimation of mexican fruit fly (diptera: tephritidae) based on accumulation of pterins.a common method of aging adult flies, fluorescence spectrometry, was used to monitor the increase of overall pterine titer in head extracts of anastrepha ludens (loew). accumulation of fluorescent compounds was measured as a function of chronological age of flies maintained at 17 and 27 degrees c. although relative fluorescence increased with age, field studies revealed that this phenomenon could not be used for accurate age estimation, as relative fluorescence did not increase predictably with ...200212539849
a new basal subfamily of mariner elements in ceratitis rosa and other tephritid flies.several copies of highly related transposable elements, crmar2, almar1, and asmar1, are described from the genomes of ceratitis rosa, anastrepha ludens, and a. suspensa, respectively. one copy from c. rosa, crmar2.5, contains a full-length, uninterrupted orf. all the other copies, from the three species contain a long deletion within the putative orf. the consensus crmar2 element has features typical of the mariner/tc1 superfamily of transposable elements. in particular, the crmar2 consensus enc ...200111677619
photoactive dye insecticide formulations: adjuvants increase toxicity to mexican fruit fly (diptera: tephritidae).the efficacy of photo active dyes as insecticides depends on the ingestion of the dye by the target insect and the activity of the dye at sensitive sites or on essential chemical functions. the site of this activity in insects is not understood, but we have found that certain chemical additives enhance the toxicity of phloxine b in the mexican fruit fly. a series oftests with commercial adjuvants was performed under laboratory conditions that demonstrated a multifold decrease in the ld50 of phlo ...200111233106
heating rate and induced thermotolerance in mexican fruit fly (diptera: tephritidae) larvae, a quarantine pest of citrus and mangoes.a bioassay and graduated temperature water baths were used to document the induction of thermotolerance in third-instar mexican fruit fly, anastrepha ludens (loew). the 99% lethal time dose for larvae exposed to 44 degrees c core temperatures in artificial fruit is 61.5 min when a slow heating rate (120 min ramp) is applied, but only 41.9 min when a fast heating rate (15 min ramp) is applied. in electrophoretic profiles a heat inducible protein of molecular weight 32 kda was detected in 76% of t ...200010985055
attractants fromstaphylococcus aureus cultures for mexican fruit fly,anastrepha ludens.volatile chemicals from tryptic soy broth cultures ofstaphylococcus aureus that attract sugar-fed, protein-hungry adult mexican fruit flies were identified. chemicals identified from the headspace above the filtrate of the bacterial cultures were ammonia, trimethylamine, isoamylamine, 2-methylbutylamine, 2,5-dimethylpyrazine, and acetic acid. each chemical attracted flies. a mixture of the chemicals in the same concentrations as were found in the bacterial filtrate was 89% as effective in attrac ...199524233835
partial characterization and hplc isolation of bacteria-produced attractants for the mexican fruit fly,anastrepha ludens.methods were developed to collect and isolate volatile chemicals produced by astaphylococcus bacterium in tryptic soy culture that are attractive to protein-hungry adult mexican fruit flies. centrifugation of bacteria culture yielded a slightly attractive pellet containing most of the bacteria cells and a highly attractive supernatant. supernatant filtered to remove the remaining bacteria was as attractive as the unfiltered supernatant. filtrate at ph 7 and above was much more attractive than fi ...199324248955
laboratory techniques for evaluating hempa and other chemosterilants against the mexican fruit fly. 19665976114
sterility in the mexican fruit fly caused by chemicals.chlorambucil, 4-{p-[bis(2-chloroethyl) amino] phenyl} butyrate, administered in food to the mexican fruit fly anastrepha iudens (loew) inhibits the growth of testes and reduces egg hatch to almost nothing. oviposition is not affected. the compound 4-amino-1h-pyrazolo (3.4-d) pyrimidine sulfate inhibits the growth of ovaries and reduces oviposition and fertility.196217732194
effects of microwave-assisted hot water treatments designed against mexican fruit fly (anastrepha ludens) on grapefruit (citrus paradisi) quality.hot water treatment (hwt) against anastrepha ludens were developed achieving 48°c in the core of grapefruits and holding it for 6 min. after heating, the grapefruits were hydro-cooled and stored at 23°c and analyzed for 16 days. the effect of microwave-assisted hot water treatment (mw-hwt) on grapefruit quality was analyzed and compared with the quality of fruits treated with hwt and control fruits (without treatment). the physico-chemical properties and chemical composition of essential oil wer ...201729250798
effect of cold storage on larval and adult anastrepha ludens (diptera: tephritidae) viability in commercially ripe, artificially infested persea americana 'hass'.commercially ripe 'hass' avocados, persea americana mill, artificially exposed to wild anastrepha ludens (loew) (diptera: tephritidae) females 24 h after harvest were placed in a cold storage facility to determine the effect of low temperature on larval survival and adult viability. fruit were left for 3, 6, 9, and 12 d in a cold room at 5 degrees c followed by a 20-25-d period at ambient temperature to allow for larval development and pupation. hass avocados and grapefruit, citrus paradisi macf ...201021309219
identifying anastrepha (diptera; tephritidae) species using dna barcodes.molecular identification of fruit flies in the genus anastrepha (diptera; tephritidae) is important to support plant pest exclusion, suppression, and outbreak eradication. morphological methods of identification of this economically important genus are often not sufficient to identify species when detected as immature life stages. dna barcoding a segment of the mitochondrial cytochrome oxidase i gene has been proposed as a method to identify pests in the genus. the identification process for the ...201729202187
effect of cryopreservation on the pre-hatching behavior in the mexican fruit fly anastrepha ludens loew (diptera, tephritidae).in a sampling of untreated embryos of the economically important fruit pest species, anastrepha ludens, the cumulative hatch percentage in the lab was noted to be ∼85%. approximately 70% of the larvae had eclosed through the posterior pole of the egg. this process is effected by the act of pole reversal (pr) of the fully developed pre-hatch larva from the wider anterior to the narrower posterior pole of the egg. investigation of the effects of cryopreservation and various pretreatments prior to ...201729273312
do mothers really know best? complexities in testing the preference-performance hypothesis in polyphagous frugivorous fruit flies.the preference-performance hypothesis (pph) has widely been used to explain host exploitation patterns by phytophagous insects. however, this hypothesis often fails in the case of polyphagous species when compared with specialists. one explanation, validated by the information-processing hypothesis (iph), considers that polyphagous insects are unable to process a large array of cues, which hinders females from distinguishing between high- and low- quality hosts. here we analyzed anastrepha luden ...201729198205
directional selection to improve the sterile insect technique: survival and sexual performance of desiccation resistant anastrepha ludens strains.the sterile insect technique (sit) is an effective, environmentally friendly method for insect control whose success depends on the sexual performance and survival of sterile males. these two parameters are influenced by environmental conditions of target areas, and releasing insects with a higher tolerance to stressful environments can improve sit efficiency. directional selection can be used to produce insect strains with higher tolerance to extreme environmental conditions, such as low humidi ...201729151857
visibility and persistence of marker dyes and effect on the quality and mating competitiveness of mass-reared flies (diptera: tephritidae): anastrepha obliqua and bisexual and genetic sexing (tapachula-7) strains of a. ludens.fluorescent dyes are commonly used in the sterile insect technique (sit) for marking insects for a proper identification after recapture. however, the quality of the mark must be balanced against insect performance, because dyes can negatively affect some parameters of insect performance and reduce their effectiveness in control with the sit. we determined the visibility and persistence and the effect of dyes on the quality of anastrepha obliqua (macquart) and anastrepha ludens (loew) (bisexual ...201728549185
reasons for success: rapid evolution for desiccation resistance and life-history changes in the polyphagous fly anastrepha ludens.species that exhibit broad ranges of distribution may successfully navigate environmental changes by modifying some of their life-history traits. environmental humidity imposes a critical stress that organisms may overcome by increasing their resistance to desiccation. we used experimental evolution to investigate adaptation to desiccation in the tephritid anastrepha ludens, a species with high fecundity, late maturation, and long lifespan. we measured morphological, physiological, developmental ...201627641541
laboratory evaluation of two commercial abamectin-based insecticides against anastrepha ludens (diptera: tephritidae): lethal and sublethal effects.toxic environmental effects of most insecticides have led to a search for bio-organic insecticides. in this study, we tested the effects of lethal and sublethal exposure to two abamectin-based insecticides (voliam targo and luq-thor) on survival, mating, and oviposition of the tephritid anastrepha ludens (loew). different doses of insecticides (0.001-2.00% of the commercial products) were offered mixed with the phagostimulant bait captor commonly used in spraying and in trapping activities for t ...201627707944
diet shapes mortality response to trauma in old tephritid fruit flies.despite the importance of trauma in healthspan and lifespan in humans as well as in non-human species, with one important exception the literature in both gerontology and ecology contains virtually no experimental demographic studies concerned with trauma in any species. we used dietary manipulation [full diet (f) versus sugar-only (s)] to produce four levels of frailty in 55-day old tephritid fruit flies (anastrepha ludens) that were then subject to the trauma of cage transfer stress (n = 900/s ...201627383645
comparison of anastrepha ludens (diptera: tephritidae) bisexual and genetic sexing (tapachula-7) strains: effect of hypoxia, fly density, chilling period, and food type on fly quality.the use of genetic sexing strain (gss) insects in the sterile insect technique (sit) makes necessary the revision of quality parameters of some stressful steps used during the packing process for aerial release because of possible differences in tolerance between fly strains. here, we determined the effect of three periods of hypoxia (12, 24, and 36 h at pupal stage), three cage densities (1.0, 1.3, and 1.5 flies/cm2), two different foods (protein/sugar (1/24) and mubarqui), and three chilling t ...201626685109
escape of mexican fruit flies from traps baited with ceratrap and effect of lure feeding on reproduction and survival.mcphail type traps are commonly used to catch tephritid pests using liquid baits as attractants. unfortunately, these traps allow that some flies escape after feeding on the bait. recently, a long-lasting and nontoxic enzymatic-hydrolyzed protein, known commercially as ceratrap, has been introduced as a novel liquid bait. because feeding on hydrolyzed proteins can improve reproduction in many tephritids, questions remain as to whether ceratrap could provide a nutritious meal to flies that ingest ...201526470313
long-term attraction and toxic effects of tephritid insecticide-bait mixtures by applying torricelli's barometer principle in a trapping device.the field activity of the mixtures of liquid baits and insecticides used in the control of tephritid pests is normally short, both when they are sprayed or when used in trapping or in attract-and-kill devices. a new lure-and-kill device based on torricelli's barometer principle was tested as a long-lasting dispenser for two liquid hydrolysed protein baits mixed with insecticide, gf-120 and captor 300 + malathion, against anastrepha ludens (loew) flies of laboratory origin. the dispensers were ke ...201626414327
sterility and sexual competitiveness of tapachula-7 anastrepha ludens males irradiated at different doses.a genetic sexing strain of anastrepha ludens (loew), tapachula-7, was developed by the mexican program against fruit flies to produce and release only males in programs where the sterile insect technique (sit) is applied. currently, breeding are found at a massive scale, and it is necessary to determine the optimum irradiation dose that releases sterile males with minimum damage to their sexual competitiveness. under laboratory and field conditions, we evaluated the effects of gamma irradiation ...201526274926
female access and diet affect insemination success, senescence, and the cost of reproduction in male mexican fruit flies anastrepha ludens.hypotheses exploring the influence of dietary conditions on the life history trade-off between survival and reproductive success are extensively tested in female insects, but are rarely explored in males. here, the impact of dietary quality and female access on age-specific reproduction and survival of male mexican fruit flies, anastrepha ludens loew (diptera: tephritidae), are examined. there is a clear cost of female access for males with access to dietary protein, measurable as a decrease in ...201525709143
estimation of populations and sterility induction in anastrepha luden (diptera: tephritidae) fruit flies.the relationship between different release densities of sterile flies and fly trap captures, expressed as flies per trap per day, in the monitoring of anastrepha ludens (loew) populations was evaluated in mango orchards. the induction of sterility in fertile females was evaluated using different ratios of sterile: fertile males under field cage conditions. a direct relationship between recaptured flies and densities of release sterile flies was found. however, trap efficiency, expressed as perce ...201425195442
susceptibility of 15 mango (sapindales: anacardiaceae) cultivars to the attack by anastrepha ludens and anastrepha obliqua (diptera: tephritidae) and the role of underdeveloped fruit as pest reservoirs: management implications.we evaluated the susceptibility of 15 mango cultivars to the attack of anastrepha ludens (loew) and anastrepha obliqua (macquart) (diptera: tephritidae), the main tephritid pests of this crop in mexico. in a field experiment, bagged fruit-bearing branches were exposed to gravid females of both fly species. infestation rates, developmental time, adult eclosion, and f1 adult longevity, fecundity, and fertility were recorded, ranking cultivars in terms of susceptibility to fly attack and developmen ...201424665723
a transgenic embryonic sexing system for anastrepha suspensa (diptera: tephritidae).the sterile insect technique (sit) is a highly successful biologically-based strategy to control pest insect populations that relies on the large-scale release of sterilized males to render females in the field non-reproductive. for medfly, a mutant-based sexing system is available as well as a transgenic system where a tetracycline-suppressible (tet-off) toxic molecule is female-specifically produced. however, the former classical genetic system took many years to refine, and the latter system ...201222858603
copulatory behaviour and the process of intromission in anastrepha ludens (diptera: tephritidae).complex genitalia occur in many arthropods and in some species extreme female morphologies lead to serious mechanical difficulties for males. tephritid flies offer examples of such complex genitalia. because of their economic importance and the extensive use of sterile male releases for tephritid control in texas and mexico, studies have been done on various aspects of their basic reproductive biology, but the process of intromission has received little attention. the distiphallus of the male of ...201121513199
comparison of in vitro heat and cold tolerances of the new invasive species bactrocera invadens (diptera: tephritidae) with three known tephritids.bactrocera invadens drew, tsuruta & white (diptera: tephritidae) is spreading throughout central africa attacking a variety of fruit; quarantines are placed on fruit from this region that are considered hosts. the only phytosanitary treatment that is commercially available is an ionizing irradiation treatment for all tephritidae at 150 gy. the development of other treatments, such as heat, cold, or fumigation, usually requires testing tens of thousands of insects at a dose that provides efficacy ...201121404834
evaluation of lufenuron as a chemosterilant against fruit flies of the genus anastrepha (diptera: tephritidae).chemosterilisation with lufenuron bait stations is a recently developed technique that is being implemented for ceratitis capitata wiedemann control. the aim of this work was to evaluate the chemosterilising effect of lufenuron against four economically important latin american fruit flies species: anastrepha ludens (loew.), a. obliqua macquart, a. serpentina wiedemann and a. striata schiner (diptera: tephritidae) in order to design a similar strategy for their control.201020157929
enriching early adult environment affects the copulation behaviour of a tephritid fly.early adult experiences in enriched environments favours animal brain and behavioural development ultimately resulting in an increased fitness. however, measuring the effect of environmental enrichment in animal behaviour in nature is often a complicated task, considering the complexity of the natural environment. we expanded previous studies to evaluate how early experience in an enriched environment affects copulation behaviour when animals are confronted with a complex semi-natural environmen ...200919525439
irradiation of anastrepha ludens (diptera: tephritidae) revisited: optimizing sterility induction.irradiation doses currently applied to sterilize mexican fruit flies, anastrepha ludens (loew) (diptera: tephritidae), for release under the sterile insect technique eradication campaign in mexico, were reviewed in an effort to increase sterile male performance in the field. a dose maximizing sterility induction into wild populations was sought by balancing somatic fitness with genetic sterility. doses of 40, 60, and 80 gy induced 95% or more sterility in all males, which in turn induced similar ...200717849864
a new blend of white sapote fruit volatiles as potential attractant to anastrepha ludens (diptera: tephritidae).the behavioral and electrophysiological responses of nonirradiated male and female anastrepha ludens (loew) (diptera: tephritidae), to white sapote, casimiroa edulis oerst. (rutaceae), volatiles were investigated. females flew upwind and landed more often on fruit than on artificial fruit in wind tunnel bioassays. males flew upwind (but not landed) more frequently on fruit than on artificial fruit. porapak q volatile extracts of white sapote also elicited upwind flight and landing on artificial ...200617195665
foraging behavior of anastrepha ludens, a. obliqua, and a. serpentina in response to feces extracts containing host marking pheromone.following oviposition, females of many tephritid flies deposit host marking pheromones (hmps) to indicate that the host fruit has been occupied. we describe the foraging behavior of these three economically important species (anastrepha ludens and a. obliqua from the fraterculus species group and a. serpentina from the serpentina species group) when they encounter an artificial fruit (green agar spheres wrapped in parafilm) marked with intra- and interspecific feces extracts that contain, among ...200616555132
biodemography of a long-lived tephritid: reproduction and longevity in a large cohort of female mexican fruit flies, anastrepha ludens.age of sexual maturity, daily and lifetime reproductive rates, and life span were recorded in a laboratory cohort of mexican fruit flies consisting of over 1100 females maintained individually. the results revealed that, relative to the medfly, the mexfly is slower maturing (14 vs 17 days), more fecund (1400 vs 650-1100 eggs/female), and longer lived (50 vs 35 days). the results reinforced the generality of several earlier findings on the medfly including the deceleration of mortality at older a ...200516154309
phylogenetic relationships among fruit flies, bactrocera (diptera, tephritidae), based on the mitochondrial rdna sequences.nucleotide sequences of a 1.6 kb long portion of the mitochondrial dna containing the majority of the 16s rrna gene, the trnaval gene, and the 5' half-region of the 12s rrna gene were determined for forty-eight individuals of nineteen bactrocera species and one other tephritid taxon, anastrepha ludens. phylogenetic analyses were performed using the consistently aligned 1.5 kb long sequences, excluding seventeen portions that could not be aligned unambiguously and were aligned inconsistently amon ...200111903624
effects of age, diet, female density, and the host resource on egg load in anastrepha ludens and anastrepha obliqua (diptera: tephritidae).oocyte counts, used as a measure of egg load, were compared among three different age groups (15, 30 and 45 days) of two polyphagous species of tephritid fruit flies, anastrepha ludens and anastrepha obliqua, which were exposed to varying conditions of diet (sucrose vs sucrose and protein), availability of oviposition substrate (present vs absent), adult female density (1, 2 and 4 females/cage), and semiochemical context (presence vs absence of male pheromones and fruit volatiles). in both speci ...200111472760
responses of laboratory-strain mexican fruit flies,anastrepha ludens, to combinations of fermenting fruit odor and male-produced pheromone in laboratory bioassays.the attractiveness toa. ludens adults of a combination of odor of fermented chapote fruit and pheromone was compared to attractiveness of both chapote and pheromone in laboratory bioassays. the chemical treatments were tested on 2-day-old and 10- to 12-day-old (sexually immature and mature, respectively), virgin and mated, nonstarved and starved, males and females during the early afternoon when flies were sexually inactive and the late afternoon when sexually mature flies were sexually active. ...199024264004
behavioral responses of female mexican fruit flies,anastrepha ludens, to components of male-produced sex pheromone.the behavioral responses of virgin female mexican fruit flies elicited by components and combinations of the components of male-produced pheromone were measured in a laboratory wind-tunnel bioassay where test chemicals were applied to the undersides of some leaves on a treated tree but to none of the leaves of a control tree. only treatments containing at least (z)-3-nonenol and/or (z,z)-3,6-nonadienol in combination with (s,s)-(-)-epianastrephin elicited strong behavioral responses. responses i ...198824276529
behavioral responses of male and female mexican fruit flies,anastrepha ludens, to male-produced chemicals in laboratory experiments.the behavioral responses of male and female mexican fruit flies elicited by male abdominal extracts were measured in laboratory cages where pheromone was applied to the undersides of some leaves on a treated tree but to none of the leaves on a control tree. after arrival to the treated tree, females came directly to pheromone sources. females on the treated tree visited leaves and fought other females at higher rates than on the control tree. females stayed on treated leaves and trees longer tha ...198624306395
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