| cathodic oxygen reduction catalyzed by bacteria in microbial fuel cells. | microbial fuel cells (mfcs) have the potential to combine wastewater treatment efficiency with energetic efficiency. one of the major impediments to mfc implementation is the operation of the cathode compartment, as it employs environmentally unfriendly catalysts such as platinum. as recently shown, bacteria can facilitate sustainable and cost-effective cathode catalysis for nitrate and also oxygen. here we describe a carbon cathode open to the air, on which attached bacteria catalyzed oxygen re ... | 2008 | 18288216 |
| exploring the ability of sphingobacterium sp. atm to degrade textile dye direct blue gll, mixture of dyes and textile effluent and production of polyhydroxyhexadecanoic acid using waste biomass generated after dye degradation. | the degradation of textile effluent using microorganisms has been studied extensively, but disposal of generated biomass after dye degradation is a serious problem. among all tested microorganisms, isolated sphingobacterium sp. atm effectively decolorized (100%) the dye direct blue gll (dbgll) and simultaneously it produced (64%) polyhydroxyhexadecanoic acid (phd). the organism decolorized dbgll at 300 mg l(-1) concentration within 24 h of dye addition and gave optimum production of phd. the org ... | 2010 | 20591565 |
| conversion of lesquerolic acid to 14-oxo-11(z)-eicosenoic acid by genetically variable sphingobacterium multivorum strains. | we investigated new microbial systems for their ability to convert lesquerolic acid (lqa; 14-hydroxy-11(z)-eicosenoic acid) to value-added products. a strain of sphingobacterium multivorum (nrrl b-23212) was found previously to convert lqa to 14-oxo-11(z)-eicosenoic acid (14-oea) as determined by gas chromatography-mass spectrometry and nuclear magnetic resonance analyses. conversion of lqa was subsequently extended to examine s. multivorum and closely related species of pedobacter, spirosoma, c ... | 2008 | 18379841 |
| diversity of cold-active protease-producing bacteria from arctic terrestrial and marine environments revealed by enrichment culture. | a new approach for enrichment culture was applied to obtain cold-active protease-producing bacteria for marine and terrestrial samples from svalbard, norway. the method was developed for the enrichment of bacteria by long-term incubation at low temperatures in semi-solid agar medium containing meat pieces as the main source of carbon and energy. zobell and 0.1x nutrient broth were added for marine and terrestrial microorganisms, respectively, to supply basal elements for growth. one to three typ ... | 2010 | 20799082 |
| sphingobacterium siyangense sp. nov., isolated from farm soil. | the taxonomic position of a novel gram-negative strain, designated sy1(t), isolated from a farm-soil sample obtained from jiangsu province, pr china, was characterized by using a polyphasic approach. the cells were non-motile, non-spore-forming rods. the organism grew optimally at 30-37 degrees c and at ph 6.0-8.0. based on 16s rrna gene sequence analysis, strain sy1(t) is a member of the genus sphingobacterium; sphingobacterium multivorum jcm 21156(t) was the nearest relative (98.5 % sequence s ... | 2008 | 18523194 |
| sphingobacterium kitahiroshimense sp. nov., isolated from soil. | a novel exopolysaccharide-degrading bacterium, designated strain 10c(t), was isolated from soil from kitahiroshima city, hokkaido, japan. the novel isolate was gram-negative, strictly aerobic and chemoheterotrophic. the dna g+c content was 36.9 mol%. major fatty acids were c(16 : 1)omega7c, iso-c(15 : 0) 2-oh, iso-c(15 : 0) and iso-c(17 : 0 )3-oh. 16s rrna gene sequence analysis and chemotaxonomic and morphological data indicated that the novel strain clearly belonged to the genus sphingobacteri ... | 2008 | 18599698 |
| presence and potential for horizontal transfer of antibiotic resistance in oxidase-positive bacteria populating raw salad vegetables. | to assess whether domestically grown fresh salad vegetables constitute a possible reservoir of antibiotic resistance for canadian consumers, aerobic bacteria capable of forming colonies at 30 degrees c on nutrient-limited media were recovered from a single sampling of romaine lettuce, savoy spinach and alfalfa sprouts, then examined for their susceptibility to ten antibiotics and the carriage of potentially mobile r-plasmids and integrons. of the 140 isolates resistant to one or more antibiotic, ... | 2008 | 18632174 |
| tetracycline resistance associated with commensal bacteria from representative ready-to-consume deli and restaurant foods. | proper knowledge of antibiotic resistance (ar) dissemination is essential for effective mitigation. this study examined the profiles of tetracycline-resistant (tetr) commensal bacteria from representative ready-to-consume food samples from salad bars at local grocery stores and restaurants. out of 900 tetr isolates examined, 158 (17.6%) carried one or more of tetm, tetl, tets, and tetk genes by conventional pcr, 28 harbored more than one tetr determinants. the most prevalent genotype was tetm, w ... | 2010 | 21067672 |
| ovarian hydrobursitis in female camels (camelus dromedaries): biochemical, bacterial and protozoal evaluation. | the aim of this study was to evaluate female camels affected with ovarian hydrobursitis (n = 31) for hematological and biochemical findings and for bacterial and protozoal infections. blood samples were obtained and surgical ablation of the affected bursa was performed. bursal fluid, follicular fluid, and serum were subjected to hormonal and biochemical analyses. bursal fluids were cultured and colonies were identified using biomérieux vitek two compact system. passive haemagglutination test was ... | 2010 | 21144565 |
| biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment. | in developing countries like india, there are many industrial areas discharging effluent containing large amount of polyaromatic hydrocarbon (pah) which causes hazardous effect on the soil-water environment. the objective of this study was to isolate and characterize high-efficiency pah-degrading microbial consortium from 3 decade old petrochemical refinery field located in nagpur, maharashtra with history of pah disposal. based on biochemical tests and 16s rdna gene sequence analysis the consor ... | 2011 | 21281999 |
| bacterial flora of captive houbara (chlamydotis undulata), kori (ardeotis kori) and rufous-crested (eupodotis ruficrista) bustards. | a survey was carried out to investigate the aerobic bacterial flora of captive houbara (chlamydotis undulata), kori (ardeotis kori) and rufous-crested (eupodotis ruficrista) bustards maintained at the national avian research center, abu dhabi, united arab emirates. none of the houbara or rufous-crested bustards sampled were bred in captivity by the center but four of the kori bustards sampled were captive-bred. faecal, cloacal, oropharyngeal and skin samples (from under the wing) from clinically ... | 1996 | 18645872 |
| bacterial diversity associated with populations of glossina spp. from cameroon and distribution within the campo sleeping sickness focus. | tsetse flies were sampled in three villages of the campo sleeping sickness focus in south cameroon. the aim of this study was to investigate the flies' gut bacterial composition using culture-dependent techniques. out of the 32 flies analyzed (27 glossina palpalis palpalis, two glossina pallicera, one glossina nigrofusca, and two glossina caliginea), 17 were shown to be inhabited by diverse bacteria belonging to the proteobacteria, the firmicutes, or the bacteroidetes phyla. phylogenetic analysi ... | 2011 | 21387098 |
| phylogenetic and metabolic bacterial diversity of phragmites australis periphyton communities in two hungarian soda ponds. | bacterial diversity of reed (phragmites australis) periphyton communities of kelemen-szék and nagy-vadas (two hungarian soda ponds) was investigated using molecular cloning and cultivation-based techniques. the majority of the 80 kelemen-szék and 72 nagy-vadas bacterial isolates proved to be moderately halophilic and alkaliphilic. a great proportion of the isolates showed phosphatase and urease activity, utilized aesculin, citrate and certain biopolymers (e.g., gelatine and tween 80). partial 16 ... | 2008 | 18679563 |
| structural changes of salinibacter sensory rhodopsin i upon formation of the k and m photointermediates. | sensory rhodopsin i (sri) is one of the most interesting photosensory receptors in nature because of its ability to mediate opposite signals depending on light color by photochromic one-photon and two-photon reactions. recently, we characterized sri from eubacterium salinibacter ruber (srsri). this protein allows more detailed information about the structure and structural changes of sri during its action to be obtained. in this paper, fourier transform infrared (ftir) spectroscopy is applied to ... | 2008 | 18991393 |
| sphingobacterium wenxiniae sp. nov., a cypermethrin-degrading species from activated sludge. | strain lqy-18t, a gram-negative, non-motile, non-spore-forming, non-flagellated, rod-shaped bacterium capable of degrading cypermethrin, was isolated from activated sludge of a waste-water treatment plant in china, and subjected to a polyphasic taxonomic investigation approach. growth occurred at 8-40 °c (optimum, 30 °c), at ph 5.0-10.0 (optimum, ph 7.0) and with 0-5 % nacl (w/v; optimum, 1 %). the predominant menaquinone was mk-7 (97 %) and the major fatty acids were summed feature 3 (comprisin ... | 2011 | 21551333 |
| a novel beta-propeller phytase from pedobacter nyackensis mj11 cgmcc 2503 with potential as an aquatic feed additive. | a phytase with high activity at neutral ph and typical water temperatures ( approximately 25 degrees c) could effectively hydrolyze phytate in aquaculture. in this study, a phytase-producing strain, pedobacter nyackensis mj11 cgmcc 2503, was isolated from glacier soil, and the relevant gene, phyp, was cloned using degenerate pcr and thermal asymmetric interlaced pcr. to our knowledge, this is the first report of detection of phytase activity and cloning of phytase gene from pedobacter. phyp belo ... | 2009 | 19139877 |
| enhanced biodegradation of pentachlorophenol in unsaturated soil using reversed field electrokinetics. | this study investigated the use of electrokinetics in unsaturated soil to promote biodegradation of pentachlorophenol through increased contact between bacteria and contaminant. soil microcosms, contaminated with approximately 100 mg kg(-1) pentachlorophenol (containing [(14)c]-pcp as a tracer), and inoculated with a specific pentachlorophenol-degrading bacterium (sphingobium sp. ug30-1 x 10(8) cfu g(-1)) were subjected to constant and regularly reversed electric currents (10 ma). the former cau ... | 2009 | 19188014 |
| sphingobacterium kyonggiense sp. nov., isolated from perchloroethylene (pce) contaminated soil and emended descriptions of sphingobacterium daejeonense and sphingobacterium mizutaii. | a gram-staining-negative, strictly aerobic, rod-shaped, non-motile strain designated 2-1-2t, was isolated from pce/tce contaminated soil in suwon, south korea. a polyphasic approach was applied to study the taxonomic position of strain 2-1-2t. strain 2-1-2t showed highest 16s rrna gene sequence similarity with sphingobacterium daejeonense tr6-04t (97.9 %) and sphingobacterium mizutaii atcc 33299t (97.1 %); sequence similarity to other sphingobacterium species was less than 93.0 %. phylogenetic a ... | 2011 | 22180612 |
| [distribution of microbes and their hereditary diversity in the rhizosphere of carnation in greenhouse]. | to investigate the relationship between microbial community and the blight diseases in rhizosphere of carnation for biological control. | 2011 | 21866707 |
| [effects of rotation and intercropping on bacterial communities in rhizosphere soil of cucumber]. | by the method of pcr-dgge, this paper studied the effects of rotation with wheat, soybean, villose vetch, clover, and alfalfa and intercropping with onion and garlic on the bacterial communities in rhizosphere soil of cucumber. the results showed that rotation and intercropping with test plants increased the diversity and evenness indices of bacterial communities in cucumber rhizosphere soil, and also, cucumber yield. the sequencing of dgge bands indicated that most of the bands had high homolog ... | 2008 | 19288729 |
| worldwide prevalence of class 2 integrases outside the clinical setting is associated with human impact. | an inti-targeted pcr assay was optimized to evaluate the frequency of partial class 2-like integrases relative to putative, environmental inti elements in clone libraries generated from 17 samples that included various terrestrial, marine, and deep-sea habitats with different exposures to human influence. we identified 169 unique inti phylotypes (< or =98% amino acid identity) relative to themselves and with respect to those previously described. among these, six variants showed an undescribed, ... | 2009 | 19502434 |
| structural insights into the enzymatic mechanism of serine palmitoyltransferase from sphingobacterium multivorum. | serine palmitoyltransferase (spt) is a key enzyme of sphingolipid biosynthesis and catalyses the pyridoxal 5'-phosphate (plp)-dependent decarboxylative condensation reaction of l-serine with palmitoyl-coa to generate 3-ketodihydrosphingosine. the crystal structure of spt from sphingobacterium multivorum gtc97 complexed with l-serine was determined at 2.3 a resolution. the electron density map showed the schiff base formation between l-serine and plp in the crystal. because of the hydrogen bond f ... | 2009 | 19564159 |
| isolates belonging to cdc group ii-i predominantly belong to sphingobacterium mizutaii (yabuuchi et al., 1983), emended description of s. mizutaii and emended description of the genus sphingobacterium. | two clinical strains, nf 296 and nf 931, present in our collection, were identified biochemically as cdc group ii-i. determination of the 16s rrna gene sequence revealed highest similarity with sphingobacterium mizutaii. because these strains produced indole, whereas s. mizutaii has been described as indole negative, we investigated also the type strain and a reference strain of s. mizutaii, i.e. lmg 8340t (ccug 15907t) and lmg 8341 (ccug 15908), and found both strains to be indole production po ... | 2011 | 22199213 |
| retinal-salinixanthin interactions in xanthorhodopsin: [corrected] a circular dichroism (cd) spectroscopy study with artificial pigments. | xanthorhodopsin (xr) is a recently discovered retinal protein that contains, in addition to the retinal chromophore, a carotenoid (salinixanthin) absorbing at 456, 486, and 520 nm, which functions as a light-harvesting antenna. we have studied the interactions between the two chromophores by monitoring the absorbance and circular dichroism (cd) spectroscopies of artificial pigments derived from synthetic retinal analogues characterized by shifted absorption maxima. in addition, we have followed ... | 2009 | 19637932 |
| nonfermenting gram-negative bacilli infections in a tertiary care hospital in kolar, karnataka. | aim: nonfermenting gram-negative bacilli (nfgnb), which are saprophytic in nature, have emerged as important healthcare-associated pathogens. they exhibit resistance not only to beta lactam and the other groups of antibiotics, but also to carbapenems. this study was undertaken to identify the nonfermenters isolated from various clinical samples, to assess their clinical significance, to know the type of healthcare-associated infections they caused, and to know their anti-microbial sensitivity pa ... | 2009 | 21938252 |
| mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus mucilaginibacter. | a non-motile, rod-shaped bacterium, designated strain wpcb133(t), was isolated from freshwater collected from the woopo wetland (republic of korea). cells were gram-reaction-negative, aerobic and catalase- and oxidase-positive. the major fatty acids were iso-c(15 : 0) and anteiso-c(15 : 0). the strain contained mk-7 as the major isoprenoid quinone. the dna g+c content was 47 mol%. a phylogenetic tree based on 16s rrna gene sequences showed that strain wpcb133(t) forms an independent lineage with ... | 2010 | 19648338 |
| Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil. | Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in a municipal solid waste (MSW) composting site. Knowledge of changes in microbial structure is useful to identify particular PAH degraders. However, the microbial community in the MSW composting soil and its change associated with prolonged exposure to PAHs and subsequent biodegradation remain largely unknown. In this study, anthracene was selected as a model compound. The bacterial community structure was investigated using termi ... | 2011 | 21887852 |
| dyadobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from soil. | a gram-negative, aerobic bacterium, designated strain bz26(t), was isolated from hydrocarbon-contaminated soil. the strain was psychrophilic, showing good growth over a temperature range of 1-20 degrees c. phylogenetic analysis based on 16s rrna gene sequences showed that strain bz26(t) was related to members of the genus dyadobacter and had highest 16s rrna gene sequence similarity to dyadobacter alkalitolerans 12116(t) (98.1 %), dyadobacter koreensis kctc 12537(t) (97.5 %) and dyadobacter gins ... | 2010 | 19717578 |
| parapedobacter luteus sp. nov. and parapedobacter composti sp. nov., isolated from cotton waste compost. | two aerobic, gram-reaction-negative, non-spore-forming bacterial strains, 4m29t and 4m40t, were isolated from cotton composts. the two strains grew in the presence of 0-5% (w/v) nacl (optimum growth in the absence of nacl), at ph 6.0-8.0 (optimum, ph 7.0) and at 15-45 degrees c (optimum, 30 degrees c). the strains shared 97.1% 16s rrna gene sequence similarity. strains 4m29t and 4m40t showed the next highest levels of 16s rrna gene sequence similarity to parapedobacter koreensis jip14t (95.6 and ... | 2010 | 19767359 |
| characterization of a signaling complex composed of sensory rhodopsin i and its cognate transducer protein from the eubacterium salinibacter ruber. | sensory rhodopsin i (sri) exists in the cell membranes of microorganisms such as the archaeon halobacterium salinarum and is a photosensor responsible for positive and negative phototaxis. sri forms a signaling complex with its cognate transducer protein, htri, in the membrane. that complex transmits light signals to the flagellar motor through changes in protein-protein interactions with the kinase chea and the adaptor protein chew, which controls the direction of the rotation of the flagellar ... | 2009 | 19778064 |
| endosymbiotic bacteria in the parasitic ciliate ichthyophthirius multifiliis. | endosymbiotic bacteria were identified in the parasitic ciliate ichthyophthirius multifiliis, a common pathogen of freshwater fish. pcr amplification of dna prepared from two isolates of i. multifiliis, using primers that bind conserved sequences in bacterial 16s rrna genes, generated an approximately 1,460-bp dna product, which was cloned and sequenced. sequence analysis demonstrated that 16s rrna gene sequences from three classes of bacteria were present in the pcr product. these included alph ... | 2009 | 19820157 |
| pedobacter bauzanensis sp. nov., isolated from soil. | a gram-negative, aerobic, rod-shaped, non-motile bacterium, designated bz42(t), was isolated from the soil of an industrial site. strain bz42(t) was able to grow at 5-25 °c. the major fatty acids were iso-c(15 : 0) (46.2 %), c(16 : 1)ω7c and/or iso-c(15 : 0) 2-oh (23.2 %) and iso-c(17 : 0) 3-oh (9.1 %). the predominant menaquinone was mk-7. the genomic dna g+c content was 36.5 mol% (hplc). 16s rrna gene sequence phylogenetic analysis revealed that strain bz42(t) was a member of the genus pedobac ... | 2010 | 20023057 |
| production of polyhydroxyhexadecanoic acid by using waste biomass of sphingobacterium sp. atm generated after degradation of textile dye direct red 5b. | the degradation of textile effluent using microorganisms has been studied extensively, but disposal of generated biomass after dye degradation is a serious problem. the isolated sphingobacterium sp. atm was found to decolorize dye direct red 5b (dr5b) and simultaneously it produced polyhydroxyhexadecanoic acid (phd). the organism decolorized dr5b at 500mgl(-1) concentration within 24h of dye addition and gave optimum production of phd. the medium contains carbon source as a molasses which was fo ... | 2010 | 20031399 |
| sphingobacterium bambusae sp. nov., isolated from soil of bamboo plantation. | a gram-negative, non-motile, non-spore-forming bacterial strain designated ibfc2009(t) was isolated from soil of a bamboo plantation. the strain could grow at 11 degrees c approximately 39 degrees c, ph 6.0-9.0, and in the presence of 0 approximately 5% nacl. based on 16s rrna gene sequence analysis, strain ibfc2009(t) belonged to the genus sphingobacterium and showed the highest sequence similarity of 94.6% (s. composti t5-12(t)) with the type strains within the genus. the major fatty acids wer ... | 2010 | 20127461 |
| pedobacter xinjiangensis sp. nov., from desert, xinjiang. | a gram-negative, rod-shaped, gliding, aerobic bacterium, designated 12157(t), was isolated from the desert of xinjiang, china and subjected to a polyphasic taxonomic study. the strain 12157(t) grew optimally at ph 7.0 and 30 degrees celsius. mk-7 was the predominant respiratory menaquinone. the dna g+c content was 42.0 mol%. phylogenetic analysis based on the 16s rrna gene sequences showed that the isolate was mostly related to the members of the genus pedobacter, with similarities ranging from ... | 2010 | 20208447 |
| influence of tetracycline exposure on the growth of wheat seedlings and the rhizosphere microbial community structure in hydroponic culture. | in this study, the effects of tetracycline exposure on wheat growth and the microbial community structure in the rhizosphere were investigated under hydroponic culture conditions. exposure to various concentrations of tetracycline resulted in significant suppression of the growth of wheat roots and shoots, with minimum doses of 0.8 mg l(-1) and 4 mg l(-1) resulting in inhibition rates of 32% and 15.4%, respectively. complete inhibition of the growth of these two parts of wheat plants was observe ... | 2010 | 20390950 |
| microbial degradation of 17beta -estradiol and 17alpha -ethinylestradiol followed by a validated hplc-dad method. | this work aimed at studying the biodegradation of two estrogens, 17alpha -estradiol (e2) and 17beta -ethinylestradiol (ee2), and their potential metabolism to estrone (e1) by microbial consortia. the biodegradation studies were followed by high performance liquid chromatography-diode array detector (hplc-dad) using a specifically developed and validated method. biodegradation studies of the estrogens (e2 and ee2) were carried out with activated sludge (consortium a, ca) obtained from a wastewate ... | 2010 | 20390962 |
| removal of heavy metals using different polymer matrixes as support for bacterial immobilisation. | great attention is focused on the microbial treatment of metal contaminated environments. three bacterial strains, 1c2, 1zp4 and ec30, belonging to genera cupriavidus, sphingobacterium and alcaligenes, respectively, showing high tolerance to zn and cd, up to concentrations of 1000ppm, were isolated from a contaminated area in northern portugal. their contribution to zn and cd removal from aqueous streams using immobilised alginate, pectate and a synthetic cross-linked polymer was assessed. in mo ... | 2011 | 21592655 |
| isolation and partial characterization of a biosurfactant mixture produced by sphingobacterium sp. isolated from soil. | strain 6.2s, isolated from soil and identified as a sphingobacterium sp., is the first strain in this genus to be reported as a biosurfactant producer, being able to reduce the surface tension of its culture supernatant to 32 mn/m. in this work, biosurfactants from the culture supernatant were purified and partially characterized. the crude extract (10 g/l) was very effective in reducing surface tension (22 mn/m). thin layer chromatography (tlc) indicated that a mixture of various biosurfactants ... | 2011 | 21684551 |
| sphingobacterium nematocida sp. nov., a nematicidal endophytic bacterium isolated from tobacco. | a novel gram-negative strain, designated m-sx103(t), was isolated from fresh leaf of nicotiana tabacum linn. collected at yuxi city, yunnan province, china, and subjected to polyphasic taxonomy. growth occurred at 10-40 °c, ph 6.0-9.0 with 0-3 % (w/v) nacl. the predominant isoprenoid quinone was mk-7. the major cellular fatty acids of strain m-sx103(t) were iso-c(15:0), iso-c(17:0) 3-oh and summed feature 3 (c(16:1)ω7c and/or c(16:1)ω6c). the g+c content of the genomic dna was 40.6 mol%. phyloge ... | 2011 | 21984669 |
| Gene cloning and characterization of a-amino acid ester acyl transferase in Empedobacter brevis ATCC14234 and Sphingobacterium siyangensis AJ2458. | The gene encoding a-amino acid ester acyl transferase (AET), the enzyme that catalyzes the peptide-forming reaction from amino acid methyl esters and amino acids, was cloned from Empedobacter brevis ATCC14234 and Sphingobacterium siyangensis AJ2458 and expressed in Escherichia coli. This is the first report on the aet gene. It encodes a polypeptide composed of 616 (ATCC14234) and 619 (AJ2458) amino acids residues. The V(max) values of these recombinant enzymes during the catalysis of L-alanyl-L- ... | 2011 | 22056425 |
| Necrotizing Fasciitis and Septic Shock Related to the Uncommon Gram-Negative Pathogen Sphingobacterium multivorum. | We report the first case of necrotizing fasciitis due to the uncommon Gram-negative pathogen Sphingobacterium multivorum in an immunocompromised patient, who presented with septic shock. This case adds necrotizing fasciitis to the spectrum of S. multivorum-related infections and highlights the emergence of Gram-negative bacteria in severe soft tissue infections. | 2012 | 22075581 |
| Use of DGGE and COMPOCHIP for investigating bacterial communities of various vermicomposts produced from different wastes under dissimilar conditions. | This study describes the use of denaturing gradient gel electrophoresis (DGGE) and COMPOCHIP (i.e. a microarray targeting typical bacteria of stabilized organic materials and pathogenic bacteria) for investigating the bacterial communities of four different vermicomposts. These included a commercial vermicompost produced from cattle manure (CM) and three vermicomposts produced at pilot-scale by recycling: damaged tomato fruits (DT); olive-mill waste mixed with biosolids (OB); and winery wastes ( ... | 2011 | 22154215 |
| the biosynthesis of the aroma volatile 2-methyltetrahydrothiophen-3-one in the bacterium chitinophaga fx7914. | 2-methyltetrahydrothiophen-3-one (3) is a volatile compound that plays an important role especially in food and flavour chemistry because it contributes to the aroma of several foodstuffs including wine. although 3 can be formed by chemical reactions during food preparation, it is also produced by microorganisms. recent studies with yeasts showed that methionine (1) is a potential precursor of 3, but the mechanism of the transformation is unknown. the biosynthetic pathway leading to 3 in the bac ... | 2010 | 20680979 |
| complete genome sequence of the representative γ-hexachlorocyclohexane-degrading bacterium sphingobium japonicum ut26. | sphingobium japonicum strain ut26 utilizes γ-hexachlorocyclohexane (γ-hch), a man-made chlorinated pesticide that causes serious environmental problems due to its toxicity and long persistence, as a sole source of carbon and energy. here, we report the complete genome sequence of ut26, which consists of two chromosomes and three plasmids. the 15 lin genes involved in γ-hch degradation are dispersed on the two chromosomes and one of the three plasmids. | 2010 | 20817768 |
| characterization of a bacterial strain capable of degrading ddt congeners and its use in bioremediation of contaminated soil. | a bacterial strain ddt-6 (d6) capable of utilizing dichlorodiphenyltrichloroethane (ddt), dichlorodiphenyldichloroethane (ddd), and dichlorodiphenyldichloroethylene (dde) (ddts) as its sole carbon and energy source was isolated and identified as sphingobacterium sp. the degradation of ddts by strain d6 in mineral salt medium and in field soil was investigated. the half-lives of the degradation of ddts increased with increasing concentration ranging from 1 to 50 mg l(-1). favorable degradation co ... | 2010 | 20828928 |
| volatile methyl esters of medium chain length from the bacterium chitinophaga fx7914. | the analysis of the volatiles released by the novel bacterial isolate chitinophaga fx7914 revealed the presence of ca. 200 compounds including different methyl esters. these esters comprise monomethyl- and dimethyl-branched, saturated, and unsaturated fatty acid methyl esters that have not been described as bacterial volatiles before. more than 30 esters of medium c-chain length were identified, which belong to five main classes, methyl (s)-2-methylalkanoates (class a), methyl (s)-2,(ω-1)-dimeth ... | 2010 | 20860026 |
| substrate specificity of haloalkane dehalogenases. | an enzyme's substrate specificity is one of its most important characteristics. the quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate-specificity data and analysis using multivariate statistics. we describe a systematic analysis of the substrate specificities of nine wild-type and four engineered haloalkane dehalogenases. the enzymes were characterized experimentally using a set of 30 ... | 2011 | 21294712 |
| [characteristics of a 3-phenoxybenzoic acid degrading-dacterium and the construction of a engineering bacterium]. | a bacterium capable of utilizing 3-phenoxybenzoic acid (3-pba) as sole carbon source was isolated from petroleum-contaminated soil. this bacterium, designated as ba3, was identified as sphingobium sp. according to its physiological & biochemical characteristic and the similarity analysis of its 16s rdna sequence. strain ba3 was able to degrade 99% of 100 mg x l(-1) 3-phenoxybenzoic acid within 60 h. the optimal ph and temperature for the degradation were 7.0 and 30 degrees c, respectively. the d ... | 2011 | 21404693 |
| characterization of heterotrophic prokaryote subgroups in the sfax coastal solar salterns by combining flow cytometry cell sorting and phylogenetic analysis. | here, we combined flow cytometry (fcm) and phylogenetic analyses after cell sorting to characterize the dominant groups of the prokaryotic assemblages inhabiting two ponds of increasing salinity: a crystallizer pond (ts) with a salinity of 390 g/l, and the non-crystallizer pond (m1) with a salinity of 200 g/l retrieved from the solar saltern of sfax in tunisia. as expected, fcm analysis enabled the resolution of high nucleic acid content (hna) and low nucleic acid content (lna) prokaryotes. next ... | 2011 | 21424516 |
| sphingobacterium lactis sp. nov. and sphingobacterium alimentarium sp. nov., isolated from raw milk and the dairy environment. | four non-fermenting, rod-shaped, gram-staining-negative, bacterial strains, designated wcc 4512t and ws 4555, wcc 4521t and ws 4556, were isolated from raw milk and the dairy environment. phylogenetic analyses based on 16s rrna and groel genes sequences demonstrated the affiliation of the four strains to two distinct clusters within the class sphingobacteriia, phylum bacteroidetes. strains wcc 4512t and ws 4555 showed the highest 16s rrna gene sequence similarity to s. daejeonense (97.3 % and 97 ... | 2011 | 21856986 |