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[intestinal infections by alcaligenes faecalis & their analogy with intestinal syndromes due to moraxella lwoffi]. 195813534033
[two cases of meningitis caused by acinetobacter lwoffi (ex moraxella)]. 195913671347
[bacteriological characteristics of 214 strains of moraxella lwoffi and m. glucidolytica (acinetobacter)]. 196113695993
[septicemic state caused by acinetobacter lwoffi]. 196013767606
[on the possible development of so-called terminal endocarditis in bacterial endocarditis. apropos of a case of endocarditis with non-glucidolytic moraxella lwoffi]. 196113871599
[multiple myocardial abscess caused by acinetobacter lwoffi]. 196213933173
gliding motility in the organisms bacterium anitratum (b5w), moraxella lwoffi and alkaligenes haemolysans, as compared to moraxella nonliquefaciens. 196314083764
[mima polymorpha (moraxella lwoffi) as the etiological agent of urethritis]. 196414134299
serology of the mima-herellea group and the genus moraxella.mitchell, paul d. (west virginia university medical center, morgantown), and robert g. burrell. serology of the mima-herellea group and the genus moraxella. j. bacteriol. 87:900-909. 1964.-the identity of organisms which have been assigned to the mima-herellea group of the tribe mimeae and their taxonomic position are uncertain. in considering the possible relationship of the mima-herellea group to the genus moraxella, the approach used was the isolation and identification of representative anti ...196414137629
[the link of bacterium anitratum, moraxella lwoffi, bacillus mallei and haemophilus parapertussis to the acinetobacter brisou and pr'evot genus]. 196414141566
[acute purulent meningitis due to moraxella lwoffi]. 195514395157
[on the systematology of bacterium anitratum, diplococcus mucosus and moraxella lwoffi. (genus acinetobacter according to brisou and prevot)]. 195914409896
[incidence of diseases caused by moraxella lwoffi and moraxella glucidolytica. study of the sensitivity to antibiotics of 28 strains isolated in new caledonia]. 195914431511
[study of 5 strains of moraxella lwoffi (acinetobacter lwoffi) isolated from pathological material]. 196114448658
[potassium ion and growth of the bacteria moraxella lwoffi]. 195014771602
[acute purulent meningitis from moraxella lwoffi; behavior of this bacteria towards antibiotics]. 195014801706
non-fermenters in human infections.one thousand and one hundred thirty non-fermenting gram negative bacteria were isolated from various samples. of these, pseudomonas aeruginosa was the commonest isolate (72.83%) followed by acinetobacter anitratus (8.4%), alcaligenes faecalis (7.6%), acinetobacter lwoffi (4.4%), pseudomonas flourescens (2.4%), schwanella putrefaciens (1.6%), stenotrophomonas maltophilia (1.6%), pseudomonas putida (0.4%), bravundimonas vesicularis (0.4%) and flavobacterium meningosepticum (0.4%). antibiotic sensi ...200315022936
trauma-induced acinetobacter lwoffi endophthalmitis with multi-organism recurrence: strategies with intravitreal treatment.endophthalmitis and its treatment are challenging, controversial subjects. we report here a patient whose recurrent endophthalmitis required several modes of treatment.19979537147
cloning and characterization of two cata genes in acinetobacter lwoffii k24.two novel type i catechol 1,2-dioxygenases inducible on aniline media were isolated from acinetobacter lwoffii k24. although the two purified enzymes, cd i1 and cd i2, had similar intradiol cleavage activities, they showed different substrate specificities for catechol analogs, physicochemical properties, and amino acid sequences. two cata genes, cata1 and cata2, encoding by cd i1 and cd i2, respectively, were isolated from the a. lwoffii k24 genomic library by using colony hybridization and pcr ...19979260969
phosphate release and uptake by pure cultures of acinetobacter sp.: effect of the volatile fatty acids concentrationphosphorus release and uptake by pure cultures of acinetobacter strains were investigated under anaerobic and aerobic conditions respectively. tests were performed to study the relationship between phosphorus release-storage reaction and behavior of extracellular organic substrates: acetic, propionic, and butyric acids have been used at four concentrations (50, 100, 500, and 1000 mg · l-1) in the anaerobic step of biological phosphorus removal. the results obtained depend on the strain an ...19978939801
utilization and degradation of an ester-based synthetic lubricant by acinetobacter lwoffi.an oil-degrading bacterium, acinetobacter lwoffi, isolated by elective culture from the medway estuary, utilized an ester-based synthetic lubricating oil emkarate de 155 as sole carbon and energy source. analysis of culture supernatants by gas chromatography showed the accumulation of a nondegradable metabolite 1,1,1 tris (hydroxymethyl)propane in addition to two metabolizable fatty acids, octanoic and decanoic acids as products of the synthetic oil degradation. esterase activities were subseque ...19968882802
characterization of the chromosomal cephalosporinases produced by acinetobacter lwoffii and acinetobacter baumannii clinical isolates.the beta-lactamases produced by acinetobacter lwoffii ula-501, acinetobacter baumannii ula-187, and a. baumannii ac-14 strains were purified and characterized, and their kinetic interactions with several beta-lactam molecules, including substrates and inhibitors, were studied in detail. the three enzymes appeared to be cephalosporinases with different acylation efficiencies (kcat/km ratio values), and their hydrolytic activities were inhibited by benzylpenicillin, piperacillin, and cefotaxime, w ...19968851599
isolation, characterization, and sequence analysis of cryptic plasmids from acinetobacter calcoaceticus and their use in the construction of escherichia coli shuttle plasmids.three cryptic plasmids have been discovered in acinetobacter calcoaceticus bd413. these three plasmids, designated pwm10 (7.4 kb), pwm11 (2.4 kb), and pwm12 (2.2 kb), exhibited extensive homology to one another, as shown by southern blot hybridization and restriction site analysis data, and also hybridized with three plasmids having slightly different sizes detected in a second strain, a. calcoaceticus bd4. plasmid pwm11 and a fragment of pwm10 were each subcloned into puc19, yielding plasmids p ...19938215356
endotoxin in the occupational environment of bakers: method of detection.the present study was conducted in a large mechanical city bakery. the as-50 aspirators equipped with cellulose membrane filters were used for dust sampling. airborne microbial content was assessed by means of sedimentation and aspiration using an andersen sampler on petri plates containing mcconkey's medium. the following gram-negative rods were detected in the bakery atmosphere: erwinia herbicola, acinetobacter lwoffi and klebsiella oxytoca, in concentrations ranging from 1.4 10(4) to 3.5 10(5 ...19947820386
gut bacteria provide precursors of benzodiazepine receptor ligands in a rat model of hepatic encephalopathy.benzodiazepine receptor (bzr) ligands are elevated in animals and humans with fulminant hepatic failure (fhf) and contribute to the pathogenesis of the associated hepatic encephalopathy (he). as gut factors are proposed to play a role in the pathogenesis of he, we investigated gut flora as a source of bzr ligands. rats received daily oral neomycin, vancomycin and metronidazole (ab +) or saline (ab -) before and concurrent with the induction of fhf with thioacetamide. bzr ligands were extracted f ...19957648264
bacterial pneumonia in recipients of bone marrow transplantation. a five-year prospective study.bacterial pneumonia as an important complication of bone marrow transplantation (bmt) has not been subjected to comprehensive analysis. two hundred fifty-five consecutive allogeneic and autologous bmt recipients, ranging in age from 1 month to 53 years, were prospectively followed for 3 days to 3 years (median, 108 days) for development of bacterial pneumonia. etiology, place acquired, chest radiography, and outcome were recorded and the association between bacterial pneumonia and demographic an ...19957570975
detection of alpha/beta-hydrolase fold in the cell surface esterases of acinetobacter species using an analysis of 3d profiles.the primary sequence of esterases from acinetobacter lwoffii rag-1 and a. calcoaceticus bd413 were compared with linearized structural sequences of two hundred proteins selected from brookhaven protein databank using a modified version of the bowie et al. algorithm [3]. significant structural homology was found to alpha/beta proteins and specifically to those with the alpha/beta-hydrolase fold for which the crystal structure was reported. no such homology was detected using common primary sequen ...19957556598
phylogenetic relationships between some members of the genera neisseria, acinetobacter, moraxella, and kingella based on partial 16s ribosomal dna sequence analysis.we obtained 16s ribosomal dna (rdna) sequence data for strains belonging to 11 species of proteobacteria, including the type strains of kingella kingae, neisseria lactamica, neisseria meningitidis, moraxella lacunata subsp. lacunata, [neisseria] ovis, moraxella catarrhalis, moraxella osloensis, [moraxella] phenylpyruvica, and acinetobacter lwoffii, as well as strains of neisseria subflava and acinetobacter calcoaceticus. the data in a distance matrix constructed by comparing the sequences suppor ...19947520730
successful treatment of late onset infection due to multi-drug resistant acinetobacter lwoffii in a low birth weight neonate using ciprofloxacin.this report presents the case of a low birth weight neonate with multidrug-resistant acinetobacter lwoffii infection who was successfully treated with ciprofloxacin and co-trimoxazole. use of ciprofloxacin in pediatric populations was reviewed. the infant responded to the antibiotic regimen with sterilized cerebrospinal fluid with no adverse effects attributable to the ciprofloxacin. although ciprofloxacin has been found to cause irreversible damage to cartilage in laboratory animals, a review o ...200111556475
a direct pathway for the conversion of propionate into pyruvate in moraxella lwoffi.1. the identity of the organism previously known as vibrio o1 (n.c.i.b. 8250) with a species of moraxella is established. 2. the ability of cells to oxidize propionate is present only in cells with an endogenous respiration and this ability is increased 80-fold when the organism is grown with propionate. 3. isocitrate lyase activity in extracts from propionate-grown cells is the same as that in extracts from lactate-grown cells, about tenfold greater than that in extracts from succinate-grown ce ...19684296055
in vitro susceptibility of acinetobacter species to various antimicrobial agents.the in vitro activity of 18 antimicrobial agents against 40 clinical isolates each of acinetobacter calcoaceticus subsp. anitratum and acinetobacter lwoffi was studied. most of the newer 4-quinolone derivatives were extremely active against these organisms. newer beta-lactam agents, such as cefpirome, bmy 28142, and brl 36650, were also extremely active, inhibiting all strains at clinically achievable levels. most agents were two- to fourfold more active against a. lwoffi.19863800353
characterization of aloi, a restriction-modification system of a new type.we report the properties of the new aloi restriction and modification enzyme from acinetobacter lwoffi ks 4-8 that recognizes the dna target 5' gga(n)6gttc3' (complementary strand 5' gaac(n)6tcc3'), and the nucleotide sequence of the gene encoding this enzyme. aloi is a bifunctional large polypeptide (deduced m(r) 143 kda) revealing both dna endonuclease and methyltransferase activities. depending on reaction cofactors, aloi cleaves double-stranded dna on both strands, seven bases on the 5' side ...200111718555
in vitro activity of imipenem towards gram-negative bacilli.the authors studied the in vitro antimicrobial activity of imipenem towards 355 gram-negative bacterial strains, taking into particular consideration unusual or dangerous species. the study was carried out on a comparative basis with piperacillin, cefotaxime, ceftazidime and gentamicin. ninety percent of the fermentative gram-negative strains were inhibited at concentrations less than or equal to 2 mg/l. imipenem inhibited 100% of strains of alcaligenes faecalis, alcaligenes denitrificans, flavo ...19863466723
isolation of acinetobacter lwoffi from hens with septicemia.acinetobacter lwoffi was isolated from the lungs of two hens. the isolate was sensitive to chloramphenicol, ampicillin, erythromycin, oxytetracycline but resistant to penicillin and nitrofurantoin.19892750363
structure of the peptidoglycans of moraxella glucidolytica and moraxella lwoffi grown on hydrocarbons.the peptidoglycans of moraxella glucidolytica and moraxella lwoffi grown on aliphatic hydrocarbons were isolated. they contained muramic acid, glucosamine, alanine, d-glutamic acid and mesodiaminoimelic acid in a molar ratio of about 0.5:0.5:1.6:1.0:1.0 (m. glucidolytica) and 0.8:0.7:1.3:1.0:1.0 (m. lwoffi). the peptidoglycans were lysozyme-resistant. however, when treated with formanide, they could be partially degraded by lysozyme. the fragments were purified and their structure determined. in ...1977871229
distribution and in vitro antimicrobial susceptibility of acinetobacter species on the skin of healthy humans.acinetobacter spp. are ubiquitous in the environment and have emerged as important nosocomial pathogens. the distribution of acinetobacter spp. in some temperate european countries has been reported. however, similar data from a tropical country such as india are not available.200111547525
bacterial flora of hirudo medicinalis and their antibiotic sensitivities in the middle black sea region, turkey.the rate of infectious complications of leech therapy is almost 20% because hirudo medicinalis has endosymbiotic bacteria. the aim of this study was to determine the bacterial flora of h. medicinalis and their antibiotic sensitivities in a region in turkey. sixteen adult leeches were collected in middle black sea region, turkey. they were rubbed onto blood agar plates directly under ether anesthesia to obtain surface cultures. they were then killed to obtain mouth and gut cultures. culture swabs ...200111756807
particulate malate oxidation in strictly aerobic bacteria: the respiratory chain of moraxella lwoffi. 197211946616
gastritis and hypergastrinemia due to acinetobacter lwoffii in mice.in mouse models and humans, helicobacter pylori is associated with an increase in serum gastrin and gastrin-expressing (g) cells with a concomitant decrease in somatostatin-expressing d cells. inflammation of the gastric mucosa can progress to metaplastic changes in the stomach and to decreased colonization by h. pylori and increased colonization by non-h. pylori organisms. in addition, about 20% of individuals with chronic gastritis are h. pylori negative, suggesting that other organisms may in ...200211953405
antimicrobial multiresistance in bacteria isolated from freshwater chilean salmon farms.the intensive use of antimicrobial agents, mainly oxytetracycline, to prevent and control bacterial pathologies in chilean salmon culture is a frequent practice. a total of 103 gram-negative oxytetracycline-resistant bacteria recovered from various sources of 4 chilean freshwater salmon farms were identified and investigated for their susceptibility patterns to various antibacterial agents, by using an agar disk diffusion method. antibacterial resistance patterns of isolates were not correlated ...200212109474
compositions and the in vitro antimicrobial activities of the essential oils of achillea setacea and achillea teretifolia (compositae).gc-ms analysis of the isolated essential oils from air-dried aerial parts of achillea setacea and achillea teretifolia, an endemic taxon, resulted in the identification of 51 constituents (79.8% of the total oil) and 42 constituents (87.1% of the total oil), respectively. eucalyptol (1,8-cineole) was the major constituent of both oils studied (18.5 and 19.9%, respectively). the antimicrobial activities of the essential oils were individually evaluated against 14 microorganisms. both oils exhibit ...200212413716
bacteremia in cirrhotic patients submitted to endoscopic band ligation of esophageal varices.endoscopic procedures can develop bacteremia. patients with chronic liver disease are more predisposed to undergo bacteremia and infections because they are immunocompromised.200415029392
proteomic analysis of acinetobacter lwoffii k24 by 2-d gel electrophoresis and electrospray ionization quadrupole-time of flight mass spectrometry.the ms/ms analysis by electrospray ionization quadrupole-time of flight mass spectrometry (esi-q-tof ms) was applied to identify proteins in proteome analysis of bacteria whose genomes are not known. the protein identification by esi-q-tof ms was performed sequentially by database search and then de novo sequencing using ms/ms spectra. soil bacteria having unanalyzed genome, acinetobacter lwoffii k24 is an aniline degrading bacterium. in this report, we present the results of a comparison betwee ...200415134882
in vitro antimicrobial activity of gatifloxacin compared with other quinolones against clinical isolates from cancer patients.owing to the predominance of gram-positive pathogens in neutropenic cancer patients, newer generation quinolones with an expanded gram-positive spectrum and enhanced potency, may have a role to play for prophylaxis and/or empiric therapy in such patients. the in vitro activity of gatifloxacin was compared with that of ciprofloxacin, levofloxacin and trovafloxacin against 848 recent clinical isolates from cancer patients. against gram-positive organisms, gatifloxacin was the most active agent tes ...200415523180
inactivation of gram-negative and gram-positive bacteria in red cell concentrates using inactine pen110 chemistry.the risk of transfusion-transmitted bacterial infections as a result of the presence of bacteria in blood is one of the major concerns in transfusion medicine. the purpose of this study was to investigate whether bacteria inoculated into red blood cell concentrates can be inactivated by the inactine pen110 pathogen-reduction process. four bacterial species were chosen for the study: anaerobic gram-positive clostridium perfringens and propionibacterium acnes, known to be transfusion-transmitted; ...200415569065
bacteriospermia in extended porcine semen.bacteriospermia is a frequent finding in freshly extended porcine semen and can result in detrimental effects on semen quality and longevity if left uncontrolled. the primary source of bacterial contamination is the boar. other sources that have been identified include environment, personnel, and the water used for extender preparation. a 1-year retrospective study was performed on submissions of extended porcine semen for routine quality control bacteriological screening at the university of pe ...200515626417
design and application of an oligonucleotide microarray for the investigation of compost microbial communities.a microarray consisting of oligonucleotide probes targeting variable regions of the 16s rrna gene was designed and tested for the investigation of microbial communities in compost. probes were designed for microorganisms that have been previously reported in the composting process and for plant, animal and human pathogens. the oligonucleotide probes were between 17 and 25 bp in length and included mostly species-specific sequences. validation of probe specificity and optimization of hybridizatio ...200515823393
primary cutaneous cryptococcosis and secondary antigenemia in a patient with long-term corticosteroid therapy.we report a case of a 71-year-old male who developed severe cellulitis of his right forearm and hand after he had an accidental injury from the sharp edge of a metal plate of a birdhouse. the patient suffers from chronic asthma and has been treated with systemic corticosteroids for years. culture of aspirates from two sites of the wound area revealed growth of cryptococcus neoformans in one and acinetobacter lwoffii in the other. after combined treatment including antibiotics, antifungal therapy ...200515827877
gram-negative bacilli associated with catheter-associated and non-catheter-associated bloodstream infections and hand carriage by healthcare workers in neonatal intensive care units.bloodstream infections caused by gram-negative bacilli are a substantial cause of morbidity and mortality in infants in neonatal intensive care units. this study describes the species of gram-negative bacilli causing bloodstream infections in two neonatal intensive care units, compares characteristics of catheter-related and non-catheter-related bloodstream infections, and compares species and antibiotic resistance patterns of these organisms with those isolated from the hands of nurses working ...200515982435
isolation of acinetobacter lwoffii from a lovebird (agapornis roseicollis) with severe respiratory symptoms.although acinetobacter lwoffii is generally considered an ubiquitous and opportunistic bacterium, this germ has been isolated from the pulmonary and abdominal air sac swabs obtained from a lovebird (agapornis roseicollis), which died of a severe respiratory disease. bacteriological tests (phenotypic and genotypic) led to the identification of a. lwoffii in pure culture. all the other parrots in the breeding centre were treated orally with oxytetracycline for 14 days and 3 months later no bird sh ...200515999637
preseptal cellulitis caused by acinetobacter lwoffi. 200516137978
differential organization and transcription of the cat2 gene cluster in aniline-assimilating acinetobacter lwoffii k24.catabc genes encode proteins that are responsible for the first three steps of one branch of the beta-ketoadipate pathway involved in the degradation of various aromatic compound by bacteria. aniline-assimilating acinetobacter lwoffii k24 is known to have the two-catabc gene clusters (cat1 and cat2) on the chromosome (kim et al., j. bacteriol., 179: 5226-5231, 1997). the order of the cat2 gene cluster is catb2a2c2, which has not been found in other bacteria. in this report, we analyzed the trans ...199916232607
prosthetic valve endocarditis caused by acinetobacter lwoffi: a case report and review.we are reporting a case of recurrent prosthetic-valve endocarditis (pve) caused by an unusual pathogen. the patient suffered 2 consecutive relapses of acinetobacter lwoffi bacteremia, although he had completed a full course of treatment with antibiotics to which the microorganism was susceptible. he was finally successfully operated with replacement of the infected aortic valve. acinetobacter spp are relatively low-grade but potentially virulent pathogens, and endocarditis caused by these specie ...200616371766
helicobacter pylori-secreted factors inhibit dendritic cell il-12 secretion: a mechanism of ineffective host defense.helicobacter pylori evades host immune defenses and causes chronic gastritis. immunity against intestinal pathogens is largely mediated by dendritic cells, yet the role of dendritic cells in acute h. pylori infection is largely unknown. we observed the recruitment of dendritic cells to the gastric mucosa of h. pylori-infected mice. bone marrow-derived dendritic cells from mice responded to live h. pylori by upregulating the expression of proinflammatory cytokine mrna (i.e., il-1alpha, il-1beta, ...200616469828
biodegradation of phthalate isomers by pseudomonas aeruginosa pp4, pseudomonas sp. ppd and acinetobacter lwoffii isp4.pseudomonas aeruginosa pp4, pseudomonas sp. ppd and acinetobacter lwoffii isp4 capable of utilizing phthalate isomers were isolated from the soil using enrichment culture technique. the strain isp4 metabolizes isophthalate, while ppd and pp4 utilizes all three phthalate isomers (ortho-, iso- and tere-) as the sole carbon source. isp4 utilizes isophthalate (0.1%) more rapidly (doubling time, 0.9 h) compared to ppd (4.64 h), pp4 (7.91 h) and other reported strains so far. the metabolic pathways in ...200616607524
characterization of acinetobacter from clinical isolates at gandhi memorial and associated hospitals, lucknow.the study was conducted in 4140 clinical samples sent to microbiology department from different department of g.m. and associated hospitals. the samples included 2270 urine, 960 pus, 300 blood, 210 sputum, 180 csf, 20 intercostal drainage tubes and 150 other swabs like vaginal and urethral, conjunctival smear 30, 10 ascitic fluids and 10 gastric aspirates. apart from this, 30 specimens were collected from hospitals environment, like linen and trolley. from clinical samples, 43 acinetobacter stra ...200516637397
enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms.most biofilms in their natural environments are likely to consist of consortia of species that influence each other in synergistic and antagonistic manners. however, few reports specifically address interactions within multispecies biofilms. in this study, 17 epiphytic bacterial strains, isolated from the surface of the marine alga ulva australis, were screened for synergistic interactions within biofilms when present together in different combinations. four isolates, microbacterium phyllosphaer ...200616751497
evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods.to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ...200716822636
acinetobacter lwoffii septicemia associated with a peripheral intravascular catheter. 200616847374
[acinetobacter lwoffii and anitratus]. 200717369976
pseudo-outbreak of acinetobacter lwoffii infection in a tertiary care center in thailand.we describe a pseudo-outbreak of acinetobacter lwoffii infection that was recognized early. the pseudo-outbreak involved 16 patients and occurred 3.5 months after the gns-506 vitek automated system was introduced in the microbiology laboratory. prompt confirmation of incomplete use of the automated system's algorithm as the point source of the misidentified a. lwoffii clinical isolates averted a full outbreak investigation and excess use of infection control resources.200717464933
catheter-related bacteremia and multidrug-resistant acinetobacter lwoffii. 200717479919
study of methanol-induced phenotypic changes in a novel strain of acinetobacter lwoffii.a gram-negative bacterial strain designated ls2 isolated from lahaul-spiti valley of north india was shown to produce pink pigment while utilizing methanol as sole source of carbon and energy. interestingly, pigment production was inducible in nature since the organism did not produce any pigment when grown on other carbon sources. based on phenotypic and phylogenetic characterization the non-pigmented methylotroph was identified as a novel strain of acinetobacter lwoffii mtcc 8288 (dq144736). b ...200717572881
effects of sevoflurane and/or nitrous oxide on bacterial growth in in vitro culture conditions.the aim of this study was to determine the role of sevoflurane and/or nitrous oxide on bacterial growth under conditions in vitro similar to those of clinical practice. we assessed these effects on pseudomonas aeruginosa, acinetobacter lwoffii, and staphylococcus aureus growth. bacterial inoculums were prepared from reference strains in nutritive broth. airtight chambers were filled with bacterial suspensions. each strain was studied with and without exposure to sevoflurane and/or nitrous oxide ...200717680203
chemical composition, antioxidant and antimicrobial properties of the essential oils of three salvia species from turkish flora.essential oils of three different salvia species [salvia aucheri var. aucheri (endemic), salvia aramiensis and salvia pilifera (endemic)] were screened for their possible antioxidant and antimicrobial properties as well as their chemical compositions. according to the gas chromatography (gc)/eims (gas chromatography/electron impact mass spectrum) analysis results; 41 (97.2%), 51 (98.5%) and 83 compounds (98.2%) were identified, respectively. while 1,8-cineole (30.5%), camphor (21.3%) and borneol ...200817936619
bacterial contamination of random-donor platelets in a university hospital in the midwestern region of brazil.bacterial blood contamination was probably the first recognized transfusion-transmitted disease. although the transfusion-associated bacterial sepsis has been known for a long time, it remains an important health problem. at present it is the most frequently reported cause of infectious transfusion-related fatalities. the aims of the study were to determine the prevalence of microbiologic contamination in random-donor platelets (rdps) and to identify the isolated microorganism obtained from a br ...200818005324
[electron microscopic analysis of biofilm on tracheal tubes removed from intubated neonates and the relationship between bilofilm and lower respiratory infection].mechanical ventilation support is a very important method for the salvage of serious patients. however, it can result in the formation of an adherent matrix of bacteria on the surfaces of implanted materials which is termed "biofilm". biofilm is dense bacterial communities attached to a solid surface and surrounded by an exopolysaccharide matrix. one of the most important features of bacterial biofilm is their resistance to antimicrobial agents and host immune system components. as a consequence ...200718021554
binding of nontarget microorganisms from food washes to anti-salmonella and anti-e. coli o157 immunomagnetic beads: most probable composition of background eubacteria.we present herein the composition of bacterial communities occurring in ground chicken and the changes which arise in these populations based upon nonselective partitioning by commercially-available dynal anti-salmonella and anti-e. coli o157 immunomagnetic beads (imb). our enumeration and colony selection protocol was based upon a 6 × 6 drop plate method (n = 18 for each 25-g sub-sampling) using a dilution which resulted in ca. 4-8 colonies per drop. an average of 82 ± 13 colonies were selected ...200818311564
application of pcr-sscp for molecular epidemiological studies on the exposure of farm children to bacteria in environmental dust.the environmental exposure of farm children to microorganisms in dust has become a focus of interest, since microbial exposure on farms has been related to a reduced prevalence of asthma and atopic diseases in children. previous studies almost exclusively focused on the determination of microbial counts using conventional culturing or the determination of microbial compounds i.e. endotoxins. in this study pcr-sscp (single-strand conformation polymorphism) was modified for characterising bacteria ...200818321601
the efficacy of the inorganic copper-based biocide cuwb50 is compromised by hard water.we sought to explain the unexpected failure of the inorganic copper-based biocide cuwb50 to effectively decontaminate microfibre cleaning cloths that became contaminated with acinetobacter lwoffii.200818422937
[detection methods and changes in metallo-beta-lactamase-producing gram-negative rod isolates at nagasaki university hospital from 1991 to 2005].since plasmid-mediated metallo-beta-lactamase (mbl)-producing pseudimonas aeruginosa was reported in japan, mbl-producing gram-negative rods (gnrs) have emerged worldwide. we developed a way to detect mbl-producing gnrs in routine examination using broth microdilution with the mbl inhibitor sodium mercaptoacetate (sma) in frozen plates. between 1996 and 2005, we evaluated this and other methods, including broth microdilution with another mbl inhibitor dipicolinic acid (dpa) in dry plates, conven ...200818697478
effect of bacterial interference on biofilm development by legionella pneumophila.in the ecology of legionella pneumophila a crucial role may be played by its relationship with the natural flora; thus we investigated the interactions between legionella and other aquatic bacteria, particularly within biofilms. among 80 aquatic bacteria screened for the production of bacteriocin-like substances (blss), 66.2% of them were active against l. pneumophila. the possible effect of some of these aquatic bacteria on the development and stability of l. pneumophila biofilms was studied. p ...200818769851
in vitro activity of tigecycline as a therapeutic option against multidrug-resistant acinetobacter spp.the study was performed to detect the in vitro activity of tigecycline in multidrug-resistant acinetobacter isolates from patients in hacettepe university adult hospital, turkey. the microorganisms were isolated from clinical specimens of patients with respiratory and bloodstream infections. thirty (66.7%) of the 45 inpatients were in icus. in vitro activity of imipenem, meropenem, ceftazidime, ciprofloxacin and aztreonam in 124 acinetobacter species isolated was evaluated by microdilution test. ...200819123310
galleria mellonella as a model system to study acinetobacter baumannii pathogenesis and therapeutics.nonmammalian model systems of infection such as galleria mellonella (caterpillars of the greater wax moth) have significant logistical and ethical advantages over mammalian models. in this study, we utilize g. mellonella caterpillars to study host-pathogen interactions with the gram-negative organism acinetobacter baumannii and determine the utility of this infection model to study antibacterial efficacy. after infecting g. mellonella caterpillars with a reference a. baumannii strain, we observe ...200919332683
prevalence and antimicrobial susceptibility of gram-negative bacteria isolated from bovine mastitis between 2003 and 2008 in korea.the objective of this study was to assess trends in the prevalence and distribution of gram-negative bacteria isolated from bovine mastitis and their antimicrobial susceptibilities during a 6-yr period between 2003 and 2008 in korea. escherichia coli, pseudomonas fluorescens, klebsiella pneumoniae, enterobacter cloacae, acinetobacter lwoffi/junii, pseudomonas aeruginosa, and serratia marcescens were the most commonly observed pathogens during this period. generally, gram-negative bacteria showed ...200919389959
detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in hungary.the drinking water distribution system of a hospital was investigated using standard cultivation techniques, taxon-specific pcrs targeting pathogenic bacteria, denaturing gradient gel electrophoresis, cloning and sequencing. the results obtained verify the higher sensitivity of pcr compared to cultivation for detecting legionella and pseudomonas aeruginosa. moreover, several other opportunistic pathogenic bacteria, such as escherichia albertii, acinetobacter lwoffi and corynebacterium tuberculos ...201019519854
determination of in vitro antioxidative and antimicrobial properties and total phenolic contents of ziziphora clinopodioides, cyclotrichium niveum, and mentha longifolia ssp. typhoides var. typhoides.the aerial parts of ziziphora clinopodioides, cyclotrichium niveum, and mentha longifolia ssp. typhoides var. typhoides were screened for their possible antioxidant and antimicrobial activities in addition to their penolic contents. antioxidant activity was employed by two complementary test systems: 2,2'-diphenyl-1-picrylhydrazyl free radical scavenging and beta-carotene/linoleic acid. in the first case, z. clinopodioides was superior to the other species with a 50% inhibitory concentration val ...200919627221
endemic and epidemic acinetobacter species in a university hospital: an 8-year survey.the prevalence of the currently known acinetobacter species and related trends of antimicrobial resistance in a dutch university hospital were studied. between 1999 and 2006, acinetobacter isolates from clinical samples were collected prospectively. isolates were analyzed by amplified fragment length polymorphism fingerprinting. for species identification, a profile similarity cutoff level of 50% was used, and for strain identification, a cutoff level of 90% was used. susceptibility for antimicr ...200919794057
bypassing isophthalate inhibition by modulating glutamate dehydrogenase (gdh): purification and kinetic characterization of nadp-gdhs from isophthalate-degrading pseudomonas aeruginosa strain pp4 and acinetobacter lwoffii strain isp4.pseudomonas aeruginosa strain pp4 and acinetobacter lwoffii strain isp4 metabolize isophthalate as a sole source of carbon and energy. isophthalate is known to be a competitive inhibitor of glutamate dehydrogenase (gdh), which is involved in c and n metabolism. strain pp4 showed carbon source-dependent modulation of nadp-gdh; gdh(i) was produced when cells were grown on isophthalate, while gdh(ii) was produced when cells were grown on glucose. strain isp4 produced a single form of nadp-gdh, gdh( ...201019933355
[not available]. 194720268392
structure of the o-polysaccharide of acinetobacter sp. vs-15 and acinetobacter lwoffii ek67.an o-polysaccharide was released by mild acid degradation of the lipopolysaccharide of acinetobacter sp. vs-15 and studied by chemical methods along with (1)h and (13)c nmr spectroscopy, including 2d (1)h,(1)h cosy, tocsy, roesy, (1)h,(13)c hsqc and hmbc experiments. the following structure of the pentasaccharide repeating unit of the polysaccharide was established: [formula: see text]. the o-polysaccharide of acinetobacter lwoffii ek67 was found to have the same structure.201020732679
oxa-134, a naturally occurring carbapenem-hydrolyzing class d beta-lactamase from acinetobacter lwoffii.acinetobacter lwoffii, a species whose natural habitat is the human skin, intrinsically possesses a chromosomal gene encoding a carbapenem-hydrolyzing class d β-lactamase, oxa-134. this species may therefore constitute a reservoir for carbapenemase genes that may spread among other acinetobacter species.201020837764
species identification and molecular characterization of acinetobacter spp. blood culture isolates from norway.the study investigated the species distribution, antibiotic susceptibility patterns and genotypic resistance characteristics of 113 consecutive blood culture isolates of acinetobacter species collected between 2005 and 2007 throughout norway.201121393175
improved resistance against botrytis cinerea by grapevine-associated bacteria that induce a prime oxidative burst and phytoalexin production.bacteria such as pantoea agglomerans (pa-af2), bacillus subtilis (bs-271), acinetobacter lwoffii (al-113), and pseudomonas fluorescens (pf-ct2), originating from the vineyard, can induce defense responses and enhance resistance of grapevine against the fungal pathogen botrytis cinerea. the perception of these bacteria by plant cells or tissues in relation to their activities remains unknown. in this study, we examined the relationships between the activity of each bacterium to induce or prime so ...201121425931
evaluation of the impact of dna extraction methods on bac bacterial community composition measured by denaturing gradient gel electrophoresis.aims:  the impact of dna extraction methods on biological activated carbon (bac) dna yield and bacterial community was evaluated. methods and results:  three different dna extraction methods were compared: method a, method b and method c. method c with ultrasonic pretreatment improved cell lysis efficiency (from 34% to 87%) and dna yield [from 10·58 μg g(-1) (dry wt) of carbon to 21·42 μg g(-1) (dry wt) of carbon]. denaturing gradient gel electrophoresis profiles obtained by method c recovered t ...201121535041
clinical impact and molecular basis of antimicrobial resistance in non-baumannii acinetobacter.species of acinetobacter other than acinetobacter baumannii are involved in nosocomial infections. acinetobacter lwoffii, acinetobacter genomospecies 3 and acinetobacter genomospecies 13tu are found in community- and nosocomial-acquired infections as well as in neonatal intensive care units. the non-baumannii acinetobacter are normally highly susceptible to ciprofloxacin, ampicillin/sulbactam, gentamicin and tigecycline. carbepenems show good activity although resistant isolates have been report ...201121585259
epigenetic regulation in murine offspring as a novel mechanism for transmaternal asthma protection induced by microbes.bronchial asthma is a chronic inflammatory disease resulting from complex gene-environment interactions. natural microbial exposure has been identified as an important environmental condition that provides asthma protection in a prenatal window of opportunity. epigenetic regulation is an important mechanism by which environmental factors might interact with genes involved in allergy and asthma development.201121680015
a novel influenza a h1n1 clinical manifestations in patients at chiang mai university hospital.to describe the clinical manifestations of patients affected with a novel influenza a (h1n1 2009) during the pandemic.201121863671
reliability of kirby-bauer disk diffusion method for detecting meropenem resistance among non-fermenting gram-negative bacilli.meropenem is empirically used as a last resort for the treatment of infections by non-fermenting gram-negative bacilli (nfgnb). minimum inhibitory concentration (mic) determined using agar or broth dilution methods is widely used for testing meropenem resistance. however, it is not possible in resource-poor settings.201121934220
the effect of carbon source on microbial community structure and cr(vi) reduction rate.in the present work, the effect of the carbon source on microbial community structure in batch cultures derived from industrial sludge and hexavalent chromium reduction was studied. experiments in aerobic batch reactors were carried out by amending industrial sludge with two different carbon sources: sodium acetate and sucrose. in each of the experiments performed, four different initial cr(vi) concentrations of: 6, 13, 30 and 115 mg/l were tested. the change of carbon source in the batch reacto ...201020552669
nonfermenting gram-negative bacilli infections in a tertiary care hospital in kolar, karnataka.aim: nonfermenting gram-negative bacilli (nfgnb), which are saprophytic in nature, have emerged as important healthcare-associated pathogens. they exhibit resistance not only to beta lactam and the other groups of antibiotics, but also to carbapenems. this study was undertaken to identify the nonfermenters isolated from various clinical samples, to assess their clinical significance, to know the type of healthcare-associated infections they caused, and to know their anti-microbial sensitivity pa ...200921938252
fulminant community-acquired pneumonia probably caused by acinetobacter lwoffii.we describe a case of fulminant community-acquired pneumonia probably caused by acinetobacter lwoffii. a 63-year-old woman was admitted with an acute respiratory illness and consolidation in the right upper lobe. she deteriorated rapidly despite antibiotic therapy, and required ventilatory support. the patient died of multiple organ failure and intractable shock, 7 days after admission to hospital; her disease was probably caused by a. lwoffii.201020546186
identification of the naturally occurring genes encoding carbapenem-hydrolysing oxacillinases from acinetobacter haemolyticus, acinetobacter johnsonii, and acinetobacter calcoaceticus.clin microbiol infect abstract: carbapenem resistance is increasingly being reported among acinetobacter species, and results mostly from the expression of acquired carbapenem-hydrolysing oxacillinases (chdls). several acinetobacter species intrinsically possess chromosomal chdl genes: acinetobacter baumannii (bla(oxa-51) ), acinetobacter radioresistens (bla(oxa-23) ), and acinetobacter lwoffii (bla(oxa-134) ). we aimed to identify the progenitors of novel chdl-encoding genes for identification ...201122128805
genes encoding oxa-134 like enzymes are found in acinetobacter lwoffii/ genomic species 9 and a. schindleri and can be used for identification.genes encoding bla(oxa-134) and variants were sought in 21 species of acinetobacter and found in a. lwoffii, genomic species 9 (regarded as synonyms) and a. schindleri. sequencing revealed a 9 bp deletion in the gene in the type strain of genomic species 9 (atcc 9957) relative to that in the type strain of a. lwoffii (atcc 15309). primers based on the non-deleted gene gave specific amplification of 29/30 clinical isolates of a. lwoffii/genomic species 9.201122205817
suspected seminal vesiculitis in an asian elephant (elephas maximus).a 32-year-old male asian elephant (elephas maximus) underwent routine transrectal stimulation for semen collection as part of an artificial insemination program. the procedure consisted of a preinsemination semen collection followed by two consecutive days of semen collections for artificial insemination. the second day's sample contained large numbers of inflammatory cells, intracellular bacteria, and phagocytized sperm. semen was submitted for culture and sensitivity. culture revealed acinetob ...201122950323
the use of multiplex real-time pcr improves the detection of the bacterial etiology of community acquired pneumonia.community-acquired pneumonia (cap) is still a major cause of morbidity and mortality especially to children and compromised hosts, such as the old and those with underlying chronic diseases. knowledge of pathogens causing cap constitutes the basis for selection of antimicrobial treatment. previous data have shown that etiological agents can be identified in only up to 50% of patients, but this figure can be improved by using polymerase chain reaction (pcr). this study was designed to evaluate mu ...201122433882
antimicrobial and cytotoxic effects of mexican medicinal plants.the antimicrobial effects of the mexican medicinal plants guazuma ulmifolia, justicia spicigera, opuntia joconostle, o. leucotricha, parkinsonia aculeata, phoradendron longifolium, p. serotinum, psittacanthus calyculatus, tecoma stans and teucrium cubense were tested against several human multi-drug resistant pathogens, including three gram (+) and five gram (-) bacterial species and three fungal species using the disk-diffusion assay. the cytotoxicity of plant extracts on human cancer cell line ...201122312741
acinetobacter lwoffii peritonitis in a patient receiving continuous ambulatory peritoneal dialysis. 201223379502
bla(imp) and bla(vim) mediated carbapenem resistance in pseudomonas and acinetobacter species in india.the emergence and rapid spread of blaimp and blavim metallo-beta-lactamase (mbl) producing gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options.201223277500
functional metagenomics of spacecraft assembly cleanrooms: presence of virulence factors associated with human pathogens.strict planetary protection practices are implemented during spacecraft assembly to prevent inadvertent transfer of earth microorganisms to other planetary bodies. therefore, spacecraft are assembled in cleanrooms, which undergo strict cleaning and decontamination procedures to reduce total microbial bioburden. we wanted to evaluate if these practices selectively favor survival and growth of hardy microorganisms, such as pathogens. three geographically distinct cleanrooms were sampled during the ...201627667984
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