| molecular characterization of cryptosporidium spp. in domestic pigeons (columba livia domestica) in guangdong province, southern china. | to investigate the prevalence and assess the zoonotic transmission burden of cryptosporidium species in domestic pigeons in guangdong province, southern china, 244 fecal samples were collected from four pigeon breeding farms between june 2012 and march 2013. cryptosporidium oocysts were purified by sheather's sugar flotation technique and characterized by dna sequencing of small subunit ribosomal rna (ssu rrna) gene. cryptosporidium species were determined by comparison of sequences with corresp ... | 2015 | 25773186 |
| an in vitro model of infection of chicken embryos by cryptosporidium baileyi. | cryptosporidiosis is one of the most prevalent parasitic infections in domesticated, caged and wild birds. cryptosporidium baileyi is the most common species reported in a wide range of avian hosts. although this parasite is well investigated, there is no adequate in vitro model for its endogenous development, and therefore, knowledge of each life cycle phase is scarce. in the present study, an in vitro model for c. baileyi in chicken embryos was developed and the complete life cycle investigate ... | 2014 | 25448358 |
| natural infection of cryptosporidium muris in ostriches (struthio camelus). | a total of 303 fecal samples were collected from ostriches (struthio camelus) and 31 samples (10.2%) were cryptosporidium-positive upon microscopic analysis. the infection rate was 27.6% in ostriches aged 16-60 days, 1.2% in those aged 61-180 days, and 20.4% in those aged >10 years. the cryptosporidium-positive isolates were genotyped with a restriction fragment length polymorphism analysis and dna sequence analysis of the small subunit (ssu) rrna gene. the 22 isolates from ostriches aged >10 ye ... | 2014 | 25178556 |
| diagnosis of gastric cryptosporidiosis in birds using a duplex real-time pcr assay. | three species and several genotypes of cryptosporidium can infect the epithelial surface of the bursa of fabricius, the respiratory tract, the proventriculus, the intestine, and the urinary tract in birds. there is reason to believe that gastric cryptosporidiosis in birds is caused by cryptosporidium galli and cryptosporidium avian genotype iii, resulting in a chronic illness of the proventriculus that can lead to a debilitating and fatal clinical condition in birds of the orders passeriformes a ... | 2014 | 25155280 |
| effects of different inoculation routes on the parasitic sites of cryptosporidium baileyi infection in chickens. | cryptosporidiosis is prevalent in domesticated, caged, and wild birds. cryptosporidium baileyi, an ascendant species of avian cryptosporidium, is an important pathogen. it causes respiratory disease in chickens, especially chickens younger than 50 days. in this study, sem, histological, semi-quantitative pcr, and nested pcr techniques were used to explore the impact of different inoculation routes on sites of c. baileyi infection in chickens. results showed that inoculation with sporozoites or o ... | 2014 | 25148714 |
| cryptosporidiosis in broiler chickens in zhejiang province, china: molecular characterization of oocysts detected in fecal samples. | cryptosporidium is one of the most important parasites in poultry, and this pathogen can infect more than 30 avian species. the present study investigated the infection rate of cryptosporidium among broiler chicken flocks. a total of 385 fecal samples from broiler chickens in 7 regions of zhejiang province collected from november 2010 to january 2012 were examined by microscopy. thirty-eight (10%) samples were positive for cryptosporidium infection, and 3 genotypes (cryptosporidium baileyi, cryp ... | 2014 | 25075975 |
| molecular determination and genotyping of cryptosporidium spp. in fecal and respiratory samples of industrial poultry in iran. | to identify the genotypes of prevalent cryptosporidia in broiler chickens in lorestan province, iran. | 2014 | 25063279 |
| consequence of cryptosporidiosis on the immune response of vaccinated broiler chickens against newcastle disease and/or avian influenza. | the consequence of cryptosporidiosis on the immune response of vaccinated chickens against newcastle disease and/or avian influenza was studied by using 240, 1 day old, male, white hy-line chicks and divided into 8 groups and subgroups. each group or subgroup was consisting of 30 chicks (15 × 2 replicates). the first and second groups were kept as unvaccinated control, g1uninfected and g2 infected. g3, g4 and g5 contained 2 subgroups a&b (g3a, g3b, g4a, g4b, g5a and g5b). chicks of subgroup a we ... | 2014 | 24993748 |
| spread of cryptosporidium baileyi in red grouse lagopus lagopus scoticus. | | 2014 | 24934399 |
| the expression dynamics of il-17 and th17 response relative cytokines in the trachea and spleen of chickens after infection with cryptosporidium baileyi. | cryptosporidium baileyi is the dominant cryptosporidium species in birds causing emerging health problems in the poultry industry, and is also a model to study the biology of cryptosporidium spp.. il-17 (also called il-17a) is a hallmark pro-inflammatory cytokine of th17 cells that plays an important role in several human autoimmune diseases and microbial infection disease of many animals, and it may play a role in cryptosporidium infection. | 2014 | 24886047 |
| otitis media associated with cryptosporidium baileyi in a saker falcon (falco cherrug). | a 7-week-old male saker falcon died with a history of severe refractory dyspnoea and respiratory signs. microscopical lesions included moderate to severe lymphoplasmacytic inflammation of the middle ears, conjunctivae, third eyelids, choanae, salivary glands of the tongue, turbinates, larynx, trachea, syrinx and bronchi. the lesions were associated with variable numbers of cryptosporidium spp., further confirmed by transmission electron microscopy and in-situ hybridization. cryptosporidium baile ... | 2013 | 23123131 |
| cryptosporidium baileyi--infection in red-breasted merganser (mergus serrator) ducklings from a zoological garden. | infection of the bursa of fabricius with cryptosporidium baileyi was diagnosed in a group of hand reared red-breasted merganser (mergus serrator) ducklings during one breeding season in a german zoological garden.the birds had died in an emaciated and anaemic state after problems with spontaneous feeding. the bursae were infected with moderate to high numbers of cryptosporidia, which were associated with hyperplasia, degeneration and sloughing of the affected epithelial cells and mild heterophil ... | 2012 | 23045806 |
| molecular surveillance of cryptosporidium spp., giardia duodenalis, and enterocytozoon bieneusi by genotyping and subtyping parasites in wastewater. | despite their wide occurrence, cryptosporidiosis and giardiasis are considered neglected diseases by the world health organization. the epidemiology of these diseases and microsporidiosis in humans in developing countries is poorly understood. the high concentration of pathogens in raw sewage makes the characterization of the transmission of these pathogens simple through the genotype and subtype analysis of a small number of samples. | 2012 | 22970334 |
| chick embryo tracheal organ: a new and effective in vitro culture model for cryptosporidium baileyi. | in the present study, chick embryo tracheal organ (tocs) was used to cultivate oocysts or sporozoites of cryptosporidium baileyi. approximately 5 × 10(4) sporozoites and oocysts mixture for group i; 5 × 10(5), 1 × 10(6), 2 × 10(6) purified sporozoites for group ii, group iii and group iv, respectively, were inoculated into respective chick embryo tracheal rings maintained in rpmi 1640 supplemented with 5% heat-inactivated fbs, and cultivated in each well of the 24-well culture plate at 40°c and ... | 2012 | 22520234 |
| cryptosporidium spp. in quails (coturnix coturnix japonica) in henan, china: molecular characterization and public health significance. | the prevalence of cryptosporidium spp. was investigated in scale quail (coturnix coturnix japonica) farms in henan province, china between september 2006 and august 2007. one thousand eight hundred and eighteen fecal samples from 47 quail farms in five areas were collected for the examination of cryptosporidium oocysts. the overall prevalence of cryptosporidium was 13.1% (95% ci 13.1±1.6%) (29 of 47 farms), with 72-100-day-old quails having the highest prevalence (23.6%, 95% ci 23.6±2.6%) (χ(2)= ... | 2012 | 22377447 |
| proventriculitis associated with cryptosporidium baileyi in a snowy owl (bubo scandiacus) and its epidemiological investigation. | we describe an unusual case of proventriculitis associated with cryptosporidium baileyi in a 7-wk-old snowy owl (bubo scandiacus) chick kept at a zoo. necropsy of this animal revealed diffuse mucosal thickening of the proventriculus. subsequent histopathological examinations of the proventriculus showed marked ductal epithelial hyperplasia with intestinal metaplasia and severe inflammatory cell infiltration in the lamina propria and submucosa. these lesions were associated with numerous periodic ... | 2017 | 28528556 |
| molecular diagnosis and characterization of cryptosporidium spp. in turkeys and chickens in germany reveals evidence for previously undetected parasite species. | a total of 256 fecal specimens were randomly collected from farmed poultry in germany and screened for the presence of cryptosporidium spp. by pcr and further characterized by direct automated dna sequencing. using a nested pcr amplifying approximately 830 bp 18s rdna fragment, 7.03% (n = 18) of the samples were cryptosporidium-positive. in detail, cryptosporidium was detected in 9.3% (8/86) of turkeys, 5.7% (9/158) of broilers and 8.3% (1/12) of layers. after dna sequencing, cryptosporidium par ... | 2017 | 28575116 |