| identification of a carboxylesterase associated with resistance to naled in bactrocera dorsalis (hendel). | compared to other organophosphate-resistant and -susceptible (s) lines of bactrocera dorsalis, the carboxylesterase (cbe) bde5 in the naled-resistant (nal-r) line has been found to possess remarkable quantitative elevation. our study attempts to identify the role of bde5 in naled resistance, and we discovered several points of interest. firstly, activity staining on native page revealed that the percentage of flies with intensive bde5 bands in the nal-r line was substantially higher than in the ... | 2016 | 27265823 |
| functional characterization of an α-esterase gene involving malathion detoxification in bactrocera dorsalis (hendel). | extensive use of insecticides in many orchards has prompted resistance development in the oriental fruit fly, bactrocera dorsalis (hendel). in this study, a laboratory selected strain of b. dorsalis (mr) with a 21-fold higher resistance to malathion was used to examine the resistance mechanisms to this organophosphate insecticide. carboxylesterase (care) was found to be involved in malathion resistance in b. dorsalis from the synergism bioassay by care-specific inhibitor triphenylphosphate (tpp) ... | 2016 | 27155483 |
| genetically engineered ricin suppresses bactrocera dorsalis (diptera: tephritidae) based on demographic analysis of group-reared life table. | the oriental fruit fly, bactrocera dorsalis (hendel), reduces the quantity and quality of many host fruits through the process of oviposition and larval feeding, and this insect has been considered a major insect pest in several asian countries for decades. using an earlier-developed, female-specific system that combines the toxicity of the ricin a chain (rta) and the alternative rna splicing property of doublesex (bddsx), we show that transgenic male flies harboring the rta-bddsx transgene unev ... | 2016 | 27122495 |
| larval x-ray irradiation influences protein expression in pupae of the oriental fruit fly, bactrocera dorsalis. | the sterile insect technique (sit) was developed to eradicate the new world screwworm from the southern united states and mexico, and became a component of many area-wide integrated pest management programs, particularly useful in managing tephritid fruit flies. sit is based on the idea of rearing and sterilizing male pests, originally by ionizing radiation, and then releasing into field, where they compete for and mate with wild females. mating with sterile males leads to reduced fecundity to l ... | 2016 | 27079560 |
| a nonovary-specific vitellogenin receptor from the oriental fruit fly, bactrocera dorsalis (hendel). | the yolk protein precursor, vitellogenin (vg), is absorbed into growing oocytes via receptor-mediated endocytosis for embryonic development. in this study, a vg receptor (vgr) cdna of the oriental fruit fly (bactrocera dorsalis hendel) was cloned via rt-pcr and race (genbank accession no. kr535603) and its expression analyzed. the bdvgr cdna has a length of 6,585 bp encoding 1,923 amino acids. it has a conserved motif arrangement with other insect vgrs, and showed high identity to the b. cucurbi ... | 2015 | 26280361 |
| the mitochondrial genome of the peach fruit fly, bactrocera zonata (saunders) (diptera: tephritidae): complete dna sequence, genome organization, and phylogenetic analysis with other tephritids using next generation dna sequencing. | mitochondrial genome can provide information for genomic structure as well as for phylogenetic analysis and evolutionary biology. the complete 15,935 bp mitochondrial genome of bactrocera zonata (diptera: tephritidae), is assembled from illumina miseq read data. the mitogenome information for b. zonata was compared to the homologous sequences of other tephritids. annotation indicated that the structure and orientation of 13 protein coding genes (pcgs), 22 trna and 2 rrna sequences were typical o ... | 2015 | 26031235 |
| sex-specific allometry of morphometric and reproductive traits in oriental fruit flies (diptera: tephritidae). | the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae), is a highly invasive and polyphagous pest of many horticultural crops in the world, and is currently present in asia, africa, and oceania. to provide essential knowledge for quality control in mass-rearing programs for sterile insect technique against the pest, we investigated how adult body weight and hind-tibial length were correlated in each sex and how body size of each sex affected lifetime reproductive fitness. we ... | 2016 | 26921224 |
| effects of laboratory colonization on bactrocera dorsalis (diptera, tephritidae) mating behaviour: 'what a difference a year makes'. | laboratory-reared insects are widely known to have significantly reduced genetic diversity in comparison to wild populations; however, subtle behavioural changes between laboratory-adapted and wild or 'wildish' (i.e., within one or very few generations of field collected material) populations are less well understood. quantifying alterations in behaviour, particularly sexual, in laboratory-adapted insects is important for mass-reared insects for use in pest management strategies, especially thos ... | 2015 | 26798268 |
| comparative sensitivity to methyl eugenol of four putative bactrocera dorsalis complex sibling species - further evidence that they belong to one and the same species b. dorsalis. | males of certain species belonging to the bactrocera dorsalis complex are strongly attracted to, and readily feed on methyl eugenol (me), a plant secondary compound that is found in over 480 plant species worldwide. amongst those species is one of the world's most severe fruit pests the oriental fruit fly, bactrocera dorsalis s.s., and the former taxonomic species bactrocera invadens, bactrocera papayae and bactrocera philippinensis. the latter species have been recently synonymised with bactroc ... | 2015 | 26798265 |
| gene flow and genetic structure of bactrocera carambolae (diptera, tephritidae) among geographical differences and sister species, b. dorsalis, inferred from microsatellite dna data. | the carambola fruit fly, bactrocera carambolae, is an invasive pest in southeast asia. it has been introduced into areas in south america such as suriname and brazil. bactrocera carambolae belongs to the bactrocera dorsalis species complex, and seems to be separated from bactrocera dorsalis based on morphological and multilocus phylogenetic studies. even though the carambola fruit fly is an important quarantine species and has an impact on international trade, knowledge of the molecular ecology ... | 2015 | 26798262 |
| a qpcr-based method for detecting parasitism of fopius arisanus (sonan) in oriental fruit flies, bactrocera dorsalis (hendel). | parasitism detection and species identification are necessary in fruit fly biological control. currently, release of mass-reared fopius arisanus is practiced worldwide, as it is effective in controlling bactrocera dorsalis and ceratitis capitata. to detect and assess parasitism in parasitoid mass-rearing colonies and parasitism levels in field populations across all life stages of hosts, the development of a rapid, specific and sensitive method is important. | 2015 | 25641860 |
| evaluating mating compatibility within fruit fly cryptic species complexes and the potential role of sex pheromones in pre-mating isolation. | the study of sexual behavior and the identification of the signals involved in mate recognition between con-specifics are key components that can shed some light, as part of an integrative taxonomic approach, in delimitating species within species complexes. in the tephritidae family several species complexes have received particular attention as they include important agricultural pests such as the ceratitis fasciventris (bezzi), ceratitis anonae (graham) and ceratitis rosa karsch (far) complex ... | 2015 | 26798257 |
| resolving cryptic species complexes of major tephritid pests. | an fao/iaea co-ordinated research project (crp) on "resolution of cryptic species complexes of tephritid pests to overcome constraints to sit application and international trade" was conducted from 2010 to 2015. as captured in the crp title, the objective was to undertake targeted research into the systematics and diagnostics of taxonomically challenging fruit fly groups of economic importance. the scientific output was the accurate alignment of biological species with taxonomic names; which led ... | 2015 | 26798252 |
| molecular characterization and functional analysis of bdfoxo gene in the oriental fruit fly, bactrocera dorsalis (diptera: tephritidae). | the forkhead box o transcription factor (foxo) is an important downstream transcription factor in the well-conserved insulin signaling pathway, which regulates the body size and development of insects. in this study, the foxo gene (bdfoxo) was identified from the oriental fruit fly, bactrocera dorsalis (hendel). the open reading frame of bdfoxo (2732 bp) encoded a 910 amino acid protein, and the sequence was well conserved with other insect species. the bdfoxo was highly expressed in larvae and ... | 2016 | 26701614 |
| development of a genetic sexing strain in bactrocera carambolae (diptera: tephritidae) by introgression of sex sorting components from b. dorsalis, salaya1 strain. | the carambola fruit fly, bactrocera carambolae drew & hancock is a high profile key pest that is widely distributed in the southwestern asean region. in addition, it has trans-continentally invaded suriname, where it has been expanding east and southward since 1975. this fruit fly belongs to bactrocera dorsalis species complex. the development and application of a genetic sexing strain (salaya1) of b. dorsalis sensu stricto (s.s.) (hendel) for the sterile insect technique (sit) has improved the ... | 2014 | 25471905 |
| description of a new species and new country distribution records of bactrocera (diptera: tephritidae: dacinae) from cambodia. | bactrocera (bactrocera) kohkongiae leblanc (diptera: tephritidae: dacinae), from the koh kong province of cambodia, is described as new. this species belongs to the oriental fruit fly (b. dorsalis) complex. genetic sequences (mitochondrial coi and nuclear ef1α and period) are deposited in genbank. a haplotype network, based on the coi sequences for 21 specimens, shows high genetic diversity. new country records from cambodia are included for 22 species. | 2015 | 26623878 |
| the epsilon glutathione s-transferases contribute to the malathion resistance in the oriental fruit fly, bactrocera dorsalis (hendel). | epsilon glutathione s-transferases (egsts) play important roles in xenobiotics detoxification and insecticides resistance in insects. however, the molecular mechanisms of egsts-mediated insecticide resistance remain largely unknown in the bactrocera dorsalis (hendel), one of the most notorious pests in the world. here, we investigated the roles of eight gst genes which belonged to epsilon class (bdgste1, bdgste2, bdgste3, bdgste4, bdgste5, bdgste6, bdgste7 and bdgste9) in conferring malathion re ... | 2016 | 26610787 |
| the dual oxidase gene bdduox regulates the intestinal bacterial community homeostasis of bactrocera dorsalis. | the guts of metazoans are in permanent contact with the microbial realm that includes beneficial symbionts, nonsymbionts, food-borne microbes and life-threatening pathogens. however, little is known concerning how host immunity affects gut bacterial community. here, we analyze the role of a dual oxidase gene (bdduox) in regulating the intestinal bacterial community homeostasis of the oriental fruit fly bactrocera dorsalis. the results showed that knockdown of bdduox led to an increased bacterial ... | 2016 | 26565723 |
| identification, characterization and target gene analysis of testicular micrornas in the oriental fruit fly bactrocera dorsalis. | micrornas (mirnas) are small noncoding rnas that regulate various diverse biological processes including insect spermatogenesis. the oriental fruit fly, bactrocera dorsalis, is one of the most destructive horticultural pests in east asia and the pacific region. although developmental mirna profiles of b. dorsalis have enriched our knowledge, specific testicular mirnas in this dipteran species are unexplored. in this study, we identified mirnas from b. dorsalis testes by deep sequencing, which pr ... | 2016 | 26486729 |
| the role of the transformer gene in sex determination and reproduction in the tephritid fruit fly, bactrocera dorsalis (hendel). | transformer (tra) is a switch gene in the somatic sex-determination hierarchy that regulates sexual dimorphism based on rna splicing in many insects. in tephritids, a y-linked male determining gene (m) controls sex in the sex-determination pathway. here, homologues of drosophila tra and transformer-2 (tra-2) genes were isolated and characterized in bactrocera dorsalis (hendel), one of the most destructive agricultural insect pests in many asian countries. two male-specific and one female-specifi ... | 2015 | 26481008 |
| micrornas in the oriental fruit fly, bactrocera dorsalis: extending drosophilid mirna conservation to the tephritidae. | the oriental fruit fly, bactrocera dorsalis, is an important plant pest species in the family tephritidae. it is a phytophagous species with broad host range, and while not established in the mainland united states, is a species of great concern for introduction. despite the vast amount of information available from the closely related model organism drosophila melanogaster, information at the genome and transcriptome level is still very limited for this species. small rnas act as regulatory mol ... | 2015 | 26438165 |
| effects of methyl eugenol feeding on mating compatibility of asian population of bactrocera dorsalis (diptera: tephritidae) with african population and with b. carambolae. | males of some species included in the bactrocera dorsalis complex are strongly attracted to methyl eugenol (me) (1,2-dimethoxy-4-(2-propenyl) benzene), a natural compound occurring in a variety of plant species. me feeding of males of the b. dorsalis complex is known to enhance their mating competitiveness. within b. dorsalis, recent studies show that asian and african populations of b. dorsalis are sexually compatible, while populations of b. dorsalis and bactrocera carambolae are relatively in ... | 2016 | 26362991 |
| inheritance, realized heritability, and biochemical mechanisms of malathion resistance in bactrocera dorsalis (diptera: tephritidae). | to better characterize the resistance development and therefore establish effective pest management strategies, this study was undertaken to investigate the inheritance mode and biochemical mechanisms of malathion resistance in the oriental fruit fly, bactrocera dorsalis (hendel), which is one of the most notorious pests in the world. after 22 generations of selection with malathion, the malathion-resistant (mr) strain of b. dorsalis developed a 34-fold resistance compared with a laboratory susc ... | 2016 | 26362988 |
| transcriptome analysis to identify genes for peptides and proteins involved in immunity and reproduction from male accessory glands and ejaculatory duct of bactrocera dorsalis. | in the male reproductive system of insects, the male accessory glands and ejaculatory duct (mag/ed) are important organs and their primary function is to enhance the fertility of spermatozoa. proteins secreted by the mag/ed are also known to induce post-mating changes and immunity responses in the female insect. to understand the gene expression profile in the mag/ed of the oriental fruit fly bactrocera dorsalis (hendel), that is an important pest in fruits, we performed an illumina-based deep s ... | 2016 | 26297881 |
| introduction of the rta-bddsx gene induces female-specific lethal effects in transformed bactrocera dorsalis (hendel). | the oriental fruit fly, bactrocera dorsalis (hendel), can reduce fruit production and quality and is considered to be a major insect pest in many asian countries. a system combining the toxicity of ricin and the alternative rna splicing properties of doublesex (rta-bddsx) has been proposed that results in differential sexual processing in vitro. a transgenic approach was used in this study to confirm the existence of female-specific lethal effects in vivo. | 2016 | 26269247 |
| the α6 nicotinic acetylcholine receptor subunit of frankliniella occidentalis is not involved in resistance to spinosad. | insects evolve resistance which constrains the sustainable use of insecticides. spinosyns, a class of environmentally-friendly macrolide insecticides, is not an exception. the mode of inheritance and the mechanisms of resistance to spinosad (the most common spinosyn insecticide) in frankliniella occidentalis (western flower thrips, wft) were investigated in this study. resistance (170,000-fold) was autosomal and completely recessive. recent studies showed that deletion of the nicotinic acetylcho ... | 2014 | 24861935 |
| the oriental fruitfly bactrocera dorsalis s.s. in east asia: disentangling the different forces promoting the invasion and shaping the genetic make-up of populations. | the oriental fruit fly, bactrocera dorsalis sensu stricto, is one of the most economically destructive pests of fruits and vegetables especially in east asia. based on its phytophagous life style, this species dispersed with the diffusion and implementation of agriculture, while globalization allowed it to establish adventive populations in different tropical and subtropical areas of the world. we used nine ssr loci over twelve samples collected across east asia, i.e. an area that, in relatively ... | 2014 | 24816716 |
| effect of sweeteners on the survival and behaviour of bactrocera dorsalis (hendel) (diptera: tephritidae). | the oriental fruit fly bactrocera dorsalis (hendel) (diptera: tephritidae) causes serious damage that affects fruit production. chemical insecticides have been widely used for the prevention and control of this destructive pest. however, the resistance of b. dorsalis to these compounds has become a serious problem. this study tested six sweeteners for their toxicity to b. dorsalis. | 2016 | 26177595 |
| dietary lufenuron reduces egg hatch and influences protein expression in the fruit fly bactrocera latifrons (hendel). | lufenuron (lfn), a chitin synthase inhibitor, impacts the fertility of ceratitis capitata, bactrocera dorsalis, b. cucurbitae, and b. latifrons. we posed the hypothesis that lfn curtails egg hatch in the solanaceous fruit fly, b. latifrons. in this study, newly emerged virgin adults were sexed and fed for 12 days with varying concentrations of lfn-laced agar diets until sexual maturation. eggs were collected from 12-d-old adults and the egg hatch was assessed. egg hatch decreased in adults reare ... | 2014 | 24753137 |
| discovery of chemosensory genes in the oriental fruit fly, bactrocera dorsalis. | the oriental fruit fly, bactrocera dorsalis, is a devastating fruit fly pest in tropical and sub-tropical countries. like other insects, this fly uses its chemosensory system to efficiently interact with its environment. however, our understanding of the molecular components comprising b. dorsalis chemosensory system is limited. using next generation sequencing technologies, we sequenced the transcriptome of four b. dorsalis developmental stages: egg, larva, pupa and adult chemosensory tissues. ... | 2015 | 26070069 |
| rnai-mediated knock-down of transformer and transformer 2 to generate male-only progeny in the oriental fruit fly, bactrocera dorsalis (hendel). | the transformer (tra) gene appears to act as the genetic switch that promotes female development by interaction with the transformer2 (tra-2) gene in several dipteran species including the medfly, housefly and drosophila melanogaster. in this study, we describe the isolation, expression and function of tra and tra-2 in the economically important agricultural pest, the oriental fruit fly, bactrocera dorsalis (hendel). bdtra and bdtra-2 are similar to their homologs from other tephritid species. b ... | 2015 | 26057559 |
| sequence analysis of mitochondrial nd1 gene can reveal the genetic structure and origin of bactrocera dorsalis s.s. | the oriental fruit fly, bactrocera dorsalis s.s., is one of the most important quarantine pests in many countries, including china. although the oriental fruit fly has been investigated extensively, its origins and genetic structure remain disputed. in this study, the nadh dehydrogenase subunit 1 (nd1) gene was used as a genetic marker to examine the genetic diversity, population structure, and gene flow of b. dorsalis s.s. throughout its range in china and southeast asia. | 2014 | 24655832 |
| overexpression of two α-esterase genes mediates metabolic resistance to malathion in the oriental fruit fly, bactrocera dorsalis (hendel). | esterase has been reported to be involved in malathion resistance in the oriental fruit fly, bactrocera dorsalis (hendel). however, the underlying molecular mechanism of the esterase-mediated resistance remains largely unknown in this species. here, with the use of a strain selected for malathion resistance in the laboratory (mr), we found that two overexpressed α-esterase genes, namely bdcare4 and bdcare6, predominant in the adult midgut and fat body, function in conferring malathion resistance ... | 2015 | 25940547 |
| niche overlap of congeneric invaders supports a single-species hypothesis and provides insight into future invasion risk: implications for global management of the bactrocera dorsalis complex. | the invasive fruit fly, bactrocera invadens, has expanded its range rapidly over the past 10 years. here we aimed to determine if the recent range expansion of bactrocera invadens into southern africa can be better understood through niche exploration tools, ecological niche models (enms), and through incorporating information about bactrocera dorsalis s.s., a putative conspecific species from asia. we test for niche overlap of environmental variables between bactrocera invadens and bactrocera d ... | 2014 | 24587234 |
| classical olfactory conditioning in the oriental fruit fly, bactrocera dorsalis. | the oriental fruit fly, bactrocera dorsalis, is a serious pest of fruits and vegetables. methyl eugenol (me), a male attractant, is used to against this fly by mass trapping. control effect may be influenced by learning, which could modify the olfactory response of the fly to this attractant. to collect the behavioral evidence, studies on the capability of this fly for olfactory learning are necessary. we investigated olfactory learning in male flies with a classical olfactory conditioning proce ... | 2015 | 25837420 |
| pyrosequencing reveals a shift in symbiotic bacteria populations across life stages of bactrocera dorsalis. | bactrocera dorsalis is one of the most economically important fruit flies around the world. in this study, 454 pyrosequencing was used to identify the bacteria associated with different developmental stages of b. dorsalis. at ≥ 97% nucleotide similarity, total reads could be assigned to 172 operational taxonomic units belonging to six phyla. proteobacteria dominated in immature stages while firmicutes dominated in adult stages. the most abundant families were enterococcaceae and comamondaceae. t ... | 2015 | 25822599 |
| cdna cloning and transcriptional regulation of the cecropin and attacin from the oriental fruit fly, bactrocera dorsalis (diptera: tephritidae). | we described the cdna cloning of two antimicrobial peptides (amps), cecropin (bdcec), and attacin c (bdattc), from the oriental fruit fly, bactrocera dorsalis (hendel), a serious insect pest of fruit trees. using rapid amplification of cdna ends, fragments encompassing the entire open reading frames of bdcec and bdattc were cloned and sequenced. the complete 425 bp cdna of bdcec encodes a protein of 64 amino acids with a predicted molecular weight of 6.84 kda. the 931 bp cdna of bdattc encodes a ... | 2015 | 25781309 |
| pupal x-ray irradiation influences protein expression in adults of the oriental fruit fly, bactrocera dorsalis. | the oriental fruit fly, bactrocera dorsalis, is a pest of fruit in the asia-pacific region and also, due to quarantine restrictions, a threat to california fruit production. area-wide suppression of b. dorsalis integrated several approaches including the sterile insect technique (sit). sit involves exposing juveniles to gamma radiation and releasing sterile males in substantial numbers, where they successfully compete for wild females. the resulting infertile eggs lead to reduction of the pest p ... | 2015 | 25772096 |
| endocytic pathway mediates refractoriness of insect bactrocera dorsalis to rna interference. | rna interference (rnai) is a powerful and convenient tool for sequence-specific gene silencing, and it is triggered by double-stranded rna (dsrna). rnai can be easily achieved in many eukaryotes by either injecting or feeding dsrnas. this mechanism has demonstrated its potential in fundamental research on genetics, medicine and agriculture. however, the possibility that insects might develop refractoriness to rnai remains unexplored. in this study, we report that the oriental fruit fly, bactroce ... | 2015 | 25731667 |
| discovery of genes related to formothion resistance in oriental fruit fly (bactrocera dorsalis) by a constrained functional genomics analysis. | artificial selection can provide insights into how insecticide resistance mechanisms evolve in populations. the underlying basis of such phenomena can involve complex interactions of multiple genes, and the resolution of this complexity first necessitates confirmation that specific genes are involved in resistance mechanisms. here, we used a novel approach invoking a constrained rna sequencing analysis to refine the discovery of specific genes involved in insecticide resistance. specifically, fo ... | 2015 | 25702834 |
| comparative proteomic analysis of bactrocera dorsalis (hendel) in response to thermal stress. | temperature is one of the most important environmental variables affecting growth, reproduction and distribution of insects. the rise of comparative proteomics provides a powerful tool to explore the response in proteins to thermal stress. as an important worldwide pest, the oriental fruit fly bactrocera dorsalis causes severe economic losses to crops. to understand the response of b. dorsalis to thermal stress, we performed a comparative proteome analysis of this insect after exposure to extrem ... | 2015 | 25660066 |
| humoral immunocompetence shifts in response to developmental stage change and mating access in bactrocera dorsalis hendel (diptera: tephritidae). | because immune defenses are often costly employed, insect immunocompetence cannot be always maintained at its maximum level. here, the oriental fruit fly, bactrocera dorsalis (hendel), was used as a study object to investigate how its immune defenses varied with the developmental stage change and mating access. our data indicated that both phenoloxidase (po) activity and antibacterial activity significantly increased from new larvae to pupae but decreased in adults after emergence. furthermore, ... | 2015 | 25611211 |
| an assessment of cold hardiness and biochemical adaptations for cold tolerance among different geographic populations of the bactrocera dorsalis (diptera: tephritidae) in china. | the cold hardiness of larvae, pupae, and adults of the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae) was characterized first, and then body water, total sugar and glycerol contents, and activities of superoxide dismutase (sod), catalase (cat), peroxidase (pod), and alcohol dehydrogenase (adh) of different geographical populations subjected to suitable rearing conditions and under sublethal low-temperature stress were compared. the cold hardiness of different populations ... | 2014 | 25527597 |
| field trials of solid triple lure (trimedlure, methyl eugenol, raspberry ketone, and ddvp) dispensers for detection and male annihilation of ceratitis capitata, bactrocera dorsalis, and bactrocera cucurbitae (diptera: tephritidae) in hawaii. | solid mallet tmr (trimedlure [tml], methyl eugenol [me], raspberry ketone [rk]) wafers and mallet cmr (ceralure, me, rk, benzyl acetate) wafers impregnated with ddvp (2,2-dichlorovinyl dimethyl phosphate) insecticide were measured in traps as potential detection and male annihilation technique (mat) devices. comparisons were made with 1) liquid lure and insecticide formulations, 2) solid cones and plugs with an insecticidal strip, and 3) solid single and double lure wafers with ddvp for captures ... | 2012 | 23156150 |
| insulin signaling pathway in the oriental fruit fly: the role of insulin receptor substrate in ovarian development. | insulin signaling pathways have integral roles in regulating organ growth and body size of insects. here, we identified and characterized six insulin signaling pathway components-inr, irs, pi3k92e, pi3k21b, akt, and pdk-from bactrocera dorsalis. quantitative real-time polymerase chain reaction was used to establish gene expression profiles for the insulin signaling pathway components for different developmental stages and tissues, and in response to 20-hydroxyecdysone (20e) and starvation. irs, ... | 2015 | 25499646 |
| olfactory biosensor for insect semiochemicals analysis by impedance sensing of odorant-binding proteins on interdigitated electrodes. | insects can sensitively and selectively detect thousands of semiochemicals at very low concentrations by their remarkable olfactory systems. as one of the most important olfactory proteins, odorant-binding proteins (obps) from insects are the most promising candidates for fabricating biosensors to detect biochemical molecules in the chemical ecology as well as for other biotechnological applications. in this study, we designed an olfactory biosensor by immobilizing obps from oriental fruit fly o ... | 2015 | 25453737 |
| effect of temperature on the development and survival of immature stages of the carambola fruit fly, bactrocera carambolae, and the asian papaya fruit fly, bactrocera papayae, reared on guava diet. | members of the bactrocera dorsalis hendel (diptera: tephritidae) complex constitute well-recognized destructive pests of fruits in peninsular thailand. the development and survival of immature stages of the carambola fruit fly, bactrocera carambolae drew & hancock, and the asian papaya fruit fly, bactrocera papayae drew & hancock, were compared at six constant temperatures of 15, 20, 25, 27, 30, and 35°c, 70 ± 5% relative humidity, and a photoperiod of 12:12 (l:d). the objectives were to determi ... | 2014 | 25368070 |
| characterizing the developmental transcriptome of the oriental fruit fly, bactrocera dorsalis (diptera: tephritidae) through comparative genomic analysis with drosophila melanogaster utilizing modencode datasets. | the oriental fruit fly, bactrocera dorsalis, is an important pest of fruit and vegetable crops throughout asia, and is considered a high risk pest for establishment in the mainland united states. it is a member of the family tephritidae, which are the most agriculturally important family of flies, and can be considered an out-group to well-studied members of the family drosophilidae. despite their importance as pests and their relatedness to drosophila, little information is present on b. dorsal ... | 2014 | 25348373 |
| isolation and identification of host cues from mango, mangifera indica, that attract gravid female oriental fruit fly, bactrocera dorsalis. | the oriental fruit fly, bactrocera dorsalis, is an economically damaging, polyphagous pest of fruit crops in south-east asia and hawaii, and a quarantine pest in other parts of the world. the objective of our study was to identify new attractants for b. dorsalis from overripe mango fruits. headspace samples of volatiles were collected from two cultivars of mango, 'alphonso' and 'chausa', and a strong positive behavioral response was observed when female b. dorsalis were exposed to these volatile ... | 2012 | 22438014 |
| transcriptome profiling of the testis reveals genes involved in spermatogenesis and marker discovery in the oriental fruit fly, bactrocera dorsalis. | the testis is a highly specialized tissue that plays a vital role in ensuring fertility by producing spermatozoa, which are transferred to the female during mating. spermatogenesis is a complex process, resulting in the production of mature sperm, and involves significant structural and biochemical changes in the seminiferous epithelium of the adult testis. the identification of genes involved in spermatogenesis of bactrocera dorsalis (hendel) is critical for a better understanding of its reprod ... | 2015 | 25255964 |
| mating compatibility between bactrocera invadens and bactrocera dorsalis (diptera: tephritidae). | the invasive fruit fly, bactrocera invadens drew, tsuruta & white, is a highly polyphagous fruit pest that occurs predominantly in africa yet has its origins in the indian subcontinent. it is extremely morphologically and genetically similar to the oriental fruit fly, bactrocera dorsalis (hendel); as such the specific relationship between these two species is unresolved. we assessed prezygotic compatibility between b. dorsalis and b. invadens using standardized field cage mating tests, which hav ... | 2014 | 24772542 |
| fitness costs of an insecticide resistance and their population dynamical consequences in the oriental fruit fly. | naled is a commonly used insecticide for controlling populations of the oriental fruit fly, bactrocera dorsalis (hendel), in taiwan and other countries. b. dorsalis has developed resistance to the insecticide, and the resistance management is an important issue. ecological effects (e.g., fitness costs) of the resistance, when fully understood, can be used for the resistance management. this study examined the effects of the insecticide resistance on important life history traits (i.e., survival ... | 2011 | 22299368 |
| cdna cloning and transcriptional expression profiles of a hexamerin in the oriental fruit fly, bactrocera dorsalis. | a bactrocera dorsalis hexamerin (bdar) cdna was cloned (genbank accession no. kf815528), and its transcriptional expression profiles were determined. the complete 2,530-bp cdna encodes a 780-amino acid protein with a predicted molecular mass of 94.01 kda. the proportions of phenylalanine (7.8%), tyrosine (11.2%), and methionine (2.6%) in bdar as well as all other amino acids are reported. bdar transcripts were detected in the brain, flight muscle, foregut, malpighian tubules, and fat body. in th ... | 2014 | 24740927 |
| lethal and sublethal effects of cyantraniliprole on bactrocera dorsalis (hendel) (diptera: tephritidae). | the oriental fruit fly, bactrocera dorsalis (hendel), is one of the most globally important insect pests. studies were conducted with the novel anthranilic diamide insecticide cyantraniliprole to determine its lethal and sublethal effects on b. dorsalis. | 2015 | 24700426 |
| specific volatile compounds from mango elicit oviposition in gravid bactrocera dorsalis females. | selecting a suitable oviposition site is crucial to the fitness of female insects because it determines the successful development of their offspring. during the oviposition process, an insect must use cues from the external environment to make an appropriate choice of where to lay eggs. generalist insects can detect and react to a plethora of cues, but are under selection pressure to adopt the most reliable ones to override noise and increase efficiency in finding hosts. the oriental fruit fly, ... | 2014 | 24623046 |
| oviposition site-selection by bactrocera dorsalis is mediated through an innate recognition template tuned to γ-octalactone. | innate recognition templates (irts) in insects are developed through many years of evolution. here we investigated olfactory cues mediating oviposition behavior in the oriental fruit fly, bactrocera dorsalis, and their role in triggering an irt for oviposition site recognition. behavioral assays with electrophysiologically active compounds from a preferred host, mango, revealed that one of the volatiles tested, γ-octalactone, had a powerful effect in eliciting oviposition by gravid b. dorsalis f ... | 2014 | 24465690 |
| differential gene expression in male and female fat body in the oriental fruit fly, bactrocera dorsalis. | the sexual difference in gene expression in fat body between 8- and 10-day-old male and female bactrocera dorsalis was examined using suppression subtractive hybridization. a total of 952 clones were sequenced and searched using blast from the subtracted cdna library. about 22% of these clones showed homology with detoxification enzymes including cytochrome p450 monooxygenases (cyps) and glutathione s-transferase. nadh dehydrogenases, distributed to energy metabolism, constituted about 9% of the ... | 2014 | 24338783 |
| spatial dynamics of two oriental fruit fly (diptera: tephritidae) parasitoids, fopius arisanus and diachasmimorpha longicaudata (hymenoptera: braconidae), in a guava orchard in hawaii. | we examined spatial patterns of both sexes of oriental fruit fly, bactrocera dorsalis (hendel), and its two most abundant parasitoids, fopius arisanus (sonan) and diachasmimorpha longicaudata (ashmead) in a commercial guava (psidium guajava l.) orchard. oriental fruit fly spatial patterns were initially random, but became highly aggregated with host fruit ripening and the subsequent colonization of, first, f. arisanus (egg-pupal parasitoid) and, second, d. longicaudata (larval-pupal parasitoid). ... | 2013 | 24073692 |
| performance of methyl eugenol + matrix + toxicant combinations under field conditions in hawaii and california for trapping bactrocera dorsalis (diptera: tephritidae). | the oriental fruit fly, bactrocera dorsalis (hendel) is a major pest of many fruit crops worldwide. current detection programs by federal and state agencies in the united states use a grid of traps consisting of liquid methyl eugenol (lure) and naled (toxicant) applied to cotton wicks and hung inside the trap. in recent years efforts have been made to incorporate these chemicals into various solid-type matrices that could be individually packaged to reduce human exposure to the chemicals and imp ... | 2013 | 23786060 |
| identification of host blends that attract the african invasive fruit fly, bactrocera invadens. | bactrocera invadens, an invasive fruit fly species in the afro-tropical region belonging to the bactrocera dorsalis complex, causes considerable damage to fruit production and productivity. we sought to find attractants from hosts of b. invadens that could serve as baits in traps for monitoring and management of this pest. the attractiveness of volatiles from four different fruit species (mango, guava, banana and orange) at two stages of ripeness (ripe or unripe) was tested in an olfactometer as ... | 2014 | 25236383 |
| [analysis of microsatellite loci from bactrocera dorsalis based on transcriptome dataset]. | the transcriptome database of the oriental fruit fly, bactrocera dorsalis (hendel), was used to identify the functional gene-microsatellite (est-ssr) markers and to analyze the ssr loci information. in total, 1890 est-ssr loci were identified, of which, 1296 ssr sequences could be used for primer design. the average distribution frequency of the transcriptomic ssrs was 1/10. 21 kb. however, these distribution frequencies varied considerably among different types of repeat ssrs. the tri-nucleotid ... | 2014 | 25223041 |
| nonhost status of mangosteen to bactrocera dorsalis and bactrocera carambolae (diptera: tephritidae) in thailand. | postharvest quarantine treatments (irradiation or vapor heat) are used to control fruit flies and other pests in mangosteen (garcinia mangostana l) exported to the united states and japan from thailand. no-choice tests were conducted in the laboratory to determine whether thai mangosteen is a host for bactrocera dorsalis (hendel) (oriental fruit fly) and bactrocera carambolae drew & hancock (carambola fruit fly). ripe commercial quality fruit (1 wk after harvest) that were either undamaged or da ... | 2014 | 25195422 |
| monitoring of bactrocera dorsalis (diptera: tephritidae) resistance to cyantraniliprole in the south of china. | the oriental fruit fly, bactrocera dorsalis (hendel), is a globally important economic insect pest that has evolved resistance to various types of insecticides. cyantraniliprole (dupont cyazypyr) is a new anthranilic diamide insecticide registered to control lepidopteran and sucking insects. the susceptibility of field-collected populations of b. dorsalis to cyantraniliprole was assessed via a diet incorporation bioassay in adults. based on the obtained lc50 values (ranging from 3.29 to 15.83 mi ... | 2014 | 25026687 |
| susceptibility of low-chill blueberry cultivars to mediterranean fruit fly, oriental fruit fly, and melon fly (diptera: tephritidae). | no-choice tests were conducted to determine whether fruit of southern highbush blueberry, vaccinium corymbosum l., hybrids are hosts for three invasive tephritid fruit flies in hawaii. fruit of various blueberry cultivars was exposed to gravid female flies of bactrocera dorsalis hendel (oriental fruit fly), ceratitis capitata (wiedemann) (mediterranean fruit fly), or bactrocera cucurbitae coquillet (melon fly) in screen cages outdoors for 6 h and then held on sand in the laboratory for 2 wk for ... | 2011 | 21510205 |
| thermal plasticity is related to the hardening response of heat shock protein expression in two bactrocera fruit flies. | it is generally believed that widely distributed species differ in their thermal plasticity from narrowly distributed species, but how differences in thermal plasticity are regulated at the molecular level remains largely unknown. here, we conducted a comparative study of two closely related invasive fruit fly species, bactrocera correcta and bactroceradorsalis, in china. the two species had overlapping distributions, but b. dorsalis had a much wider range throughout the country and a longer inv ... | 2014 | 24992713 |
| deep sequencing of small rna libraries reveals dynamic expression patterns of micrornas in multiple developmental stages of bactrocera dorsalis. | in eukaryotes, micrornas (mirnas) are small, conserved, noncoding rnas that have emerged as critical regulators of gene expression. the oriental fruit fly bactrocera dorsalis is one of the most economically important fruit fly pests in east asia and the pacific. although transcriptome analyses have greatly enriched our knowledge of its structural genes, little is known about post-transcriptional regulation by mirnas in this dipteran species. in this study, small rna libraries corresponding to fo ... | 2014 | 24957108 |
| prerelease exposure to methyl eugenol increases the mating competitiveness of sterile males of the oriental fruit fly (diptera: tephritidae) in a hawaiian orchard. | males of the oriental fruit fly, bactrocera dorsalis (hendel), are strongly attracted to methyl eugenol (me), and recent work demonstrated that ingestion of this chemical enhances male mating success, apparently owing its role as a precursor in the synthesis of the male sex pheromone. the current study expanded upon earlier laboratory and field-cage experiments by assessing whether prerelease exposure to me increased the mating competitiveness of mass-reared, sterile males in hawaiian orchards. ... | 2011 | 22299359 |
| regional suppression of bactrocera fruit flies (diptera: tephritidae) in the pacific through biological control and prospects for future introductions into other areas of the world. | bactrocera fruit fly species are economically important throughout the pacific. the usda, ars u.s. pacific basin agricultural research center has been a world leader in promoting biological control of bactrocera spp. that includes classical, augmentative, conservation and ipm approaches. in hawaii, establishment of bactrocera cucurbitae (coquillett) in 1895 resulted in the introduction of the most successful parasitoid, psyttalia fletcheri (silvestri); similarly, establishment of bactrocera dors ... | 2012 | 26466626 |
| toxicities and synergistic effects of several insecticides against the oriental fruit fly (diptera: tephritidae). | the oriental fruit fly, bactrocera dorsalis (hendel), is a serious insect pest that causes large losses to orchards globally. in this study, we conducted experiments to investigate the susceptibility of two populations (kunming of yunnan and dongguan of guangdong province) of b. dorsalis to nine insecticides. bioassay results demonstrated that fipronil was the most effective insecticide, followed by phoxim, abamectin, triazophos, beta-cypermethrin, chlorpyrifos, deltamethrin, malathion, and imid ... | 2013 | 23786089 |
| mating compatibility among four pest members of the bactrocera dorsalis fruit fly species complex (diptera: tephritidae). | bactrocera dorsalis (hendel), bactrocera papayae drew & hancock, bactrocera philippinensis drew & hancock, and bactrocera carambolae drew & hancock are pest members within the b. dorsalis species complex of tropical fruit flies. the species status of these taxa is unclear and this confounds quarantine, pest management, and general research. mating studies carried out under uniform experimental conditions are required as part of resolving their species limits. these four taxa were collected from ... | 2013 | 23786057 |
| multiple glutathione s-transferase genes: identification and expression in oriental fruit fly, bactrocera dorsalis. | the oriental fruit fly, bactrocera dorsalis (hendel), is widely distributed in asia-pacific regions, where it is a serious pest of a wide range of tropical and subtropical fruit and vegetable crops. in this study, 17 cdna encoding glutathione s-transferases (gsts) in b. dorsalis were sequenced and characterised. | 2014 | 23589401 |
| involvement of superoxide dismutase in oxidative stress in the oriental fruit fly, bactrocera dorsalis: molecular cloning and expression profiles. | bactrocera dorsalis, one of the most economically important fruit fly pests in east asia, is well adapted to various environmental conditions. pesticides, pathogens and other stresses can cause oxidative damage in most organisms. the superoxide dismutase (sod) family contains some of the most important enzymes in the antioxidant protection system of the fruit fly and other organisms. | 2013 | 23529838 |
| insecticide toxicity to oriental fruit fly bactrocera dorsalis (diptera: tephritidae) is influenced by environmental factors. | in this study, we investigated the effects of environmental factors (temperature, dose, dietary source, and feeding density) on the insecticide tolerance of bactrocera dorsalis (hendel) (diptera: tephritidae). the results indicated that the toxicities of trichlorphon and abamectin to b. dorsalis increased with an increase in temperature. at 15-35 degrees c, the toxicity of beta-cypermethrin decreased with an increase in temperature at low doses (0.82 and 1.86 mg/l), but was similar at a high dos ... | 2013 | 23448051 |
| biochemical and molecular characterisation of acetylcholinesterase in four field populations of bactrocera dorsalis (hendel) (diptera: tephritidae). | the oriental fruit fly, bactrocera dorsalis, is a major pest that infects fruits and agricultural products worldwide. the latest resistance monitoring of b. dorsalis from mainland china has identified high levels of resistance to insecticides. in this study, the biochemical and molecular characteristics of acetylcholinesterase (ache) in four field populations of b. dorsalis are investigated. | 2012 | 23007913 |
| population structure of bactrocera dorsalis s.s., b. papayae and b. philippinensis (diptera: tephritidae) in southeast asia: evidence for a single species hypothesis using mitochondrial dna and wing-shape data. | bactrocera dorsalis s.s. is a pestiferous tephritid fruit fly distributed from pakistan to the pacific, with the thai/malay peninsula its southern limit. sister pest taxa, b. papayae and b. philippinensis, occur in the southeast asian archipelago and the philippines, respectively. the relationship among these species is unclear due to their high molecular and morphological similarity. this study analysed population structure of these three species within a southeast asian biogeographical context ... | 2012 | 22846393 |
| mitotic and polytene chromosomes analysis of the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae). | the oriental fruit fly, batrocera dorsalis s.s. (hendel) is one of the most destructive agricultural pests, belonging to a large group of difficult to distinguish morphologically species, referred as the b. dorsalis complex. we report here a cytogenetic analysis of two laboratory strains of the species and provide a photographic polytene chromosome map from larval salivary glands. the mitotic complement consists of six chromosome pairs including a heteromorphic sex (xx/xy) chromosome pair. analy ... | 2011 | 20844937 |
| odorant receptor co-receptor orco is upregulated by methyl eugenol in male bactrocera dorsalis (diptera: tephritidae). | bactrocera dorsalis is a destructive fruit-eating pest that causes severe economic damage to the fruit and vegetable industry. methyl eugenol (me) has been widely used as an effective sexual attractant for male fruit flies through olfactory perception. however, the molecular mechanism underlying the olfactory perception of me remains unknown. here, we report the characterization and functional analysis of a newly discovered cdna that encodes a drosophila melanogaster odorant receptor co-receptor ... | 2012 | 22634470 |
| invasion history of the oriental fruit fly, bactrocera dorsalis, in the pacific-asia region: two main invasion routes. | the oriental fruit fly, bactrocera dorsalis, was initially recorded in taiwan island in 1912, and has dispersed to many areas in the pacific-asia region over the last century. the area of origin of the species may be confidently placed in south-east china. however, routes of range expansion to new areas and underlying population processes remain partially unclear, despite having been the subject of several studies. to explore the invasion history of this species, a partition of the cox1 gene of ... | 2012 | 22567138 |
| cdna isolation, expression, and hormonal regulation of yolk protein genes in the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae). | yolk protein (yp) or vitellogenin (vg), the main component of yolk, is the key nutrient for embryonic development. yps, encoded from uncleaved genes existing mainly in cyclorraphan flies, are different from vgs that are present in most non-cyclorraphan dipterans and other insects. in this study, cdnas of two yps, namely bdyp1 and bdyp2 (genbank accession nos. af368053 and af368054), were isolated in the oriental fruit fly, bactrocera dorsalis (hendel). rt-pcr analysis revealed that bdyp1 and 2 a ... | 2012 | 22349178 |
| larval dietary wheat germ oil influences age-specific protein expression in adults of the oriental fruit fly. | changes in essential dietary components alter global gene expression patterns in animals. we reported on a proteomics study designed to identify molecular markers of deficiencies in culture media developed for the oriental fruit fly, bactrocera dorsalis. in that study, we found significant changes in expression of 70 proteins in adults of larvae reared on media lacking wheat germ oil (wgo), compared to media supplemented with wgo. of these, a gene encoding an insect chitin-binding protein was ex ... | 2012 | 22326456 |
| dietary wheat germ oil influences gene expression in larvae and eggs of the oriental fruit fly. | changes in animal nutrition, particularly essential dietary components, alter global gene expression patterns. our goal is to identify molecular markers that serve as early indicators of the quality of insect culture media. markers of deficient culture media will increase the efficiency of developing optimal systems for mass rearing beneficial insects and some pest species because decisions on culture media quality can be made without waiting through one or several life cycles. the objective of ... | 2011 | 21136526 |
| host status of blueberry to invasive tephritid fruit flies in hawaii. | forced infestation studies were conducted to determine whether northern or southern highbush blueberries, vaccinium corymbosum l., are hosts for the invasive tephritid fruit flies in hawaii. fruit were exposed to gravid female flies of bactrocera dorsalis hendel (oriental fruit fly), ceratitis capitata (wiedemann) (mediterranean fruit fly), or bactrocera cucurbitae coquillet (melon fly) in screen cages outdoors for 6 h and then held on sand in the laboratory for 2 wk for pupal and adult emergenc ... | 2009 | 19886450 |
| evaluation of readmission ink as a marker for dispersal studies with the oriental fruit fly, bactrocera dorsalis. | in this text we present a new marking dye, readmission ink, blak-ray, for the purpose of insect movement studies. the dye was tested in a controlled experiment with bactrocera dorsalis (hendel) (diptera: tephritidae) in anticipation of a long distance movement study planned for the following year with the same species. 700 individuals of both sexes were marked with the dye and placed in holding containers. both the percentage of mortality and the ease of dye detection were monitored throughout a ... | 2011 | 22233346 |
| comparison of rain-fast bait stations versus foliar bait sprays for control of oriental fruit fly, bactrocera dorsalis, in papaya orchards in hawaii. | bait stations represent an environmentally friendly attract-and-kill approach to fruit fly population suppression. recently a novel, visually attractive, rain-fast bait station was developed in hawaii for potential use against multiple species of pestiferous fruit flies. here, we compared the efficacy of gf-120 nf naturalyte fruit fly bait applied either as foliar sprays or onto bait stations in reducing female oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae), population d ... | 2010 | 21067423 |
| molecular characterizations of two cytochrome p450 genes encoding cyp6a41 and cyp6ek1 from the oriental fruit fly, bactrocera dorsalis (diptera: tephritidae). | two p450 genes encoding cyp6a41 and cyp6ek1 were cloned from the oriental fruit fly using polymerase chain reaction (pcr) and rapid amplification of cdna ends (race) techniques. cyp6a41 and cyp6ek1 contained open reading frames of 1,530 and 1,524 nucleotides that encode 510 and 508 amino acid residues, respectively. the putative proteins shared 44% identity with each other. phylogenetic analysis showed that cyp6a41 and cyp6ek1 were most closely related to ceratitis capitata cyp6a10 and cyp6a sub ... | 2012 | 23589219 |
| resveratrol modifies tephritid fruit fly response to radiation but not nutritional stress. | resveratrol (3,5,4'-trihydroxystilbene) is a polyphenol compound found in many plants and fruits that has antioxidant and radioprotective properties. two model invertebrates, bactrocera dorsalis (oriental fruit fly) and b. cucurbitae (melon fly) (diptera: tephritidae), were studied to determine if the addition of resveratrol to an artificial diet could modify their response to radiation and nutritional stress. | 2012 | 22145646 |
| cdna cloning and characterization of s6 kinase and its effect on yolk protein gene expression in the oriental fruit fly bactrocera dorsalis (hendel). | p70 s6 kinase (s6k), a serine/threonine protein kinase, is a downstream target of target of rapamycin (tor) gene and an important regulator of protein synthesis responsible for cell growth and reproduction. in this study, a s6k gene, named bds6k (genbank accession no. gq203802), was isolated from the oriental fruit fly bactrocera dorsalis (hendel). quantitative rt-pcr showed that bds6k mrna is expressed at a higher level in egg than in other developmental stages, as well as in ovary than in fat ... | 2011 | 22105664 |
| bioefficacy of alpinia galanga (zingiberaceae) rhizome extracts, (e)-p-acetoxycinnamyl alcohol, and (e)-p-coumaryl alcohol ethyl ether against bactrocera dorsalis (diptera: tephritidae) and the impact on detoxification enzyme activities. | the application of insecticides to control oriental fruit fly, bactrocera dorsalis hendel (diptera: tephritidae), is a principal component of the current management of these fruit flies. however, we evaluated four extracts of alpinia galanga wild linn (zingiberaceae) rhizomes against adult flies and found hexane and ethanol extracts to be most effective (lc50 = 4,866 and 6,337 ppm, respectively, after 24 h). this suggested that both nonpolar and polar compounds could be active in the candidate p ... | 2011 | 22066182 |
| effectiveness of attract-and-kill systems using methyl eugenol incorporated with neonicotinoid insecticides against the oriental fruit fly (diptera: tephritidae). | laboratory bioassays and field trials were conducted to evaluate an "attract-and-kill" system using methyl eugenol (me) with neonicotinoid insecticides against male oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae). in laboratory bioassays, mortality of male flies resulting from the conventional toxicant, naled was 98.3-100% at 24 through 72 h after treatment, whereas the neonicotinoid insecticides imidacloprid and acetamiprid caused only approximately 60-80% at 24 through ... | 2008 | 18459398 |
| the utility of microsatellite dna markers for the evaluation of area-wide integrated pest management using sit for the fruit fly, bactrocera dorsalis (hendel), control programs in thailand. | the oriental fruit fly, bactrocera dorsalis (hendel), is a key pest that causes reduction of the crop yield within the international fruit market. fruit flies have been suppressed by two area-wide integrated pest management programs in thailand using sterile insect technique (aw-ipm-sit) since the late 1980s and the early 2000s. the projects' planning and evaluation usually rely on information from pest status, distribution, and fruit infestation. however, the collected data sometimes does not p ... | 2011 | 21052785 |
| the oriental fruit fly, bactrocera dorsalis, in china: origin and gradual inland range expansion associated with population growth. | the oriental fruit fly, bactrocera dorsalis, expanded throughout mainland china in the last century to become one of the most serious pests in the area, yet information on this process are fragmentary. three mitochondrial genes (nad1, cytb and nad5) were used to infer the genetic diversity, population structure and demographic history of the oriental fruit fly from its entire distribution range in china. high levels of genetic diversity, as well as a significant correspondence between genetic an ... | 2011 | 21984907 |
| isolation and characterization of doublesex homologues in the bactrocera species: b. dorsalis (hendel) and b. correcta (bezzi) and their putative promoter regulatory regions. | doublesex (dsx) is a double-switch gene at the bottom of the somatic sex-determination hierarchy which regulates sexual dimorphism in many insects. here, drosophila melanogaster homologues of dsx were isolated in two bactrocera species, the oriental fruit fly, b. dorsalis, and the guava fruit fly, b. correcta. results of rt-pcr analysis suggests that both the b. dorsalis dsx (bd1dsx) and b. correcta dsx (bcdsx) genes are transcribed and sex-specifically spliced in accordance with the drosophila ... | 2011 | 20976560 |
| can ménage-a-trois be used for controlling insects? | we propose a new cross-species disruption approach that might be capable of interrupting mating of one species that uses another insect species as the mercenary agent. we argue that insects treated with a sufficiently powerful attractant for a second species might interfere with mating of one or both species, for example, by leading males astray in pursuit of the false trails created by suitably dosing individuals of the first species. our reciprocal test systems used (1) methyl eugenol, an attr ... | 2007 | 17619106 |
| accumulation of phenylpropanoid and sesquiterpenoid volatiles in male rectal pheromonal glands of the guava fruit fly, bactrocera correcta. | the guava fruit fly, bactrocera correcta, is widely distributed in thailand and other surrounding southeast asian countries, and, like the closely related sympatric species, the oriental fruit fly, b. dorsalis, infests various fruits, including guava, peach, and mango. males of both b. correcta and b. dorsalis are strongly attracted to, and compulsively feed on, methyl eugenol (me). bactrocera dorsalis males fed on me sequester its metabolite phenylpropanoids, (e)-coniferyl alcohol and 2-allyl-4 ... | 2010 | 20967492 |
| a comparative assessment of the response of three fruit fly species (diptera: tephritidae) to a spinosad-based bait: effect of ammonium acetate, female age, and protein hunger. | ammonia-releasing substances are known to play an important role in fruit fly (diptera: tephritidae) attraction to food sources, and this information has been exploited for the development of effective synthetic food-based lures and insecticidal baits. in field studies conducted in hawaii, we examined the behavioural response of wild female oriental fruit fly (bactrocera dorsalis (hendel)), melon fly (b. cucurbitae (coquillett)), and mediterranean fruit fly (ceratitis capitata (wiedemann)) to sp ... | 2011 | 20961468 |
| evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly bactrocera dorsalis (diptera: tephritidae). | quantitative real-time reverse transcriptase pcr (rt-qpcr) has been widely used for quantification of mrna as a way to determine key genes involved in different biological processes. for accurate gene quantification analysis, normalization of rt-qpcr data is absolutely essential. to date, normalization is most frequently achieved by the use of internal controls, often referred to as reference genes. however, several studies have shown that the reference genes used for the quantification of mrna ... | 2010 | 20923571 |
| area-wide suppression of the mediterranean fruit fly, ceratitis capitata, and the oriental fruit fly, bactrocera dorsalis, in kamuela, hawaii. | the united states department of agriculture's agricultural research service initiated an area-wide fruit fly management program in hawaii in 2000. the first demonstration site was established in kamuela, hawaii, usa. this paper documents suppression of the mediterranean fruit fly, ceratitis capitata (wiedemann), and the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae), in a 40 km2 area containing urban, rural and agricultural zones during a 6 year period. the suppression t ... | 2010 | 20883128 |
| recent advances in methyl eugenol and cue-lure technologies for fruit fly detection, monitoring, and control in hawaii. | worldwide, an important aspect of invasive insect pest management is more effective, safer detection and control systems. phenyl propanoids are attractive to numerous species of dacinae fruit flies. methyl eugenol (me) (4-allyl-1, 2-dimethoxybenzene-carboxylate), cue-lure (c-l) (4-(p-acetoxyphenyl)-2-butanone), and raspberry ketone (rk) (4-(p-hydroxyphenyl)-2-butanone) are powerful male-specific lures. most evidence suggests a role of me and c-l/rk in pheromone synthesis and mate attraction. me ... | 2010 | 20831962 |
| target of rapamycin in the oriental fruit fly bactrocera dorsalis (hendel): its cloning and effect on yolk protein expression. | target of rapamycin (tor), a serine/threonine protein kinase, is involved in regulating a number of growth and developmental processes of an organism, including yolk protein synthesis in insects. in this study, tor gene was isolated, designated bdtor (genbank accession no. fj167395), from the oriental fruit fly bactrocera dorsalis (hendel). quantitative rt-pcr showed a higher expression of bdtor in the pupa than in other developmental stages, as well as in ovary than in the fat body. downregulat ... | 2010 | 20734415 |
| wing shape as a potential discriminator of morphologically similar pest taxa within the bactrocera dorsalis species complex (diptera: tephritidae). | four morphologically cryptic species of the bactrocera dorsalis fruit fly complex (b. dorsalis s.s., b. papayae, b. carambolae and b. philippinensis) are serious agricultural pests. as they are difficult to diagnose using traditional taxonomic techniques, we examined the potential for geometric morphometric analysis of wing size and shape to discriminate between them. fifteen wing landmarks generated size and shape data for 245 specimens for subsequent comparisons among three geographically dist ... | 2012 | 21867577 |