| alteration of tomato micrornas expression during fruit development upon cucumber mosaic virus and tomato aspermy virus infection. | the economic importance of solanaceae plant species is well documented, and tomato has become a model for fleshy fruit development and ripening studies. plant micrornas (mirnas) are small endogenous rnas that are involved in a variety of activities including plant development, signal transduction and protein degradation, as well as response to environment stress and pathogen invasion. here in this study, we aimed at quantifying the expression alterations of nine mirnas and target mrnas in tomato ... | 2013 | 23277401 |
| the c-terminal domain of the 2b protein of cucumber mosaic virus is stabilized by divalent metal ion coordination. | the main function of the 2b protein of cucumber mosaic virus (cmv) is binding permanently the double stranded sirna molecules in the suppression process of post-transcriptional gene silencing (ptgs). the crystal structure of the homologue tomato aspermy virus (tav) 2b protein is known, but without the c-terminal domain. the biologically active form is a tetramer: four 2b protein molecules and two sirna duplexes. regarding the complete 2b protein structure, we performed a molecular dynamics (md) ... | 2012 | 23143042 |
| characterization of virus-encoded rna interference suppressors in caenorhabditis elegans. | in fungi, plants, and invertebrates, antiviral rna interference (rnai) directed by virus-derived small interfering rnas (sirnas) represents a major antiviral defense that the invading viruses have to overcome in order to establish infection. as a counterdefense mechanism, viruses of these hosts produce diverse classes of proteins capable of suppressing the biogenesis and/or function of viral sirnas. this rna-directed viral immunity (rdvi) in the nematode caenorhabditis elegans is known to exhibi ... | 2013 | 23468484 |
| characterization of tomato aspermy virus isolated from chrysanthemum and elucidation of its complete nucleotide sequence. | | 2015 | 26104340 |
| genetically modified tomato aspermy virus 2b protein as a tumor-targeting sirna delivery carrier. | in nature, there exist a wide range of dsrna-binding proteins that have different binding modes for small interfering rna (sirna) as well as structural differences, and some of these proteins have potential as effective sirna delivery carriers. in order to deliver sirna into cancer cells, a dsrna-binding 2b protein derived from tomato aspermy virus was genetically modified by fusing the integrin-targeting rgd peptide to its c-terminus, and biosynthesized. the resulting 2b-rgd protein possesses d ... | 2014 | 25050774 |