| fluorescent study of tobacco mosaic virus protein. | protein fluorescence properties of tobacco mosaic virus [3 trp residues per monomer (positions 17, 52, 152)] and of two tobacco mosaic virus mutants [green tomato atypical mosaic virus, 2 trp (52, 152) and cucumber virus4, 1 trp (unknown position)] have been studied. emission spectra, fluorescence quantum yields and lifetimes were determined. results showed that protein fluorescence is due to buried trp only, except for the cucumber virus4 strain, in which tyr also contributed to the emission. c ... | 1977 | 588584 |
| lipid metabolism in chlorotic spot virus infected groundnut leaves (arachis hypogaea l var. tmv 2). | | 1977 | 612535 |
| stability of mutations conferring temperature sensitivity on tobacco mosaic virus. | among several temperature-sensitive mutants of tobacco mosaic virus, reversion to wild-type phenotype is an event sufficiently rare that essentially pure stocks of mutant virus can easily be maintained. the temperature-sensitive phenotype of many of these mutants is stringently expressed. our data show that most of our mutants are suitable for use in biochemical experiments. | 1978 | 618832 |
| recovery of tobacco mosaic virus rna replication after incubation at 40 degrees. | incubation of tobacco mosaic virus (tmv)-infected tissue at 40 degrees for 12 h destroyed the capacity for tmv rna replication. upon shift of the tissue from 40 to 25 degrees, synthesis of tmv rna did not resume. incubation at 40 degrees for 1 h did not destory the tmv rna replicase, because viral rna synthesis resumed upon return to 25 degrees. however, upon further incubation at 25 degrees, the synthesis rate of tmv rna gradually declined. upon still further incubation at 25 degrees (16-20 h), ... | 1978 | 621137 |
| time-course of actinomycin d inhibition of tobacco mosaic virus multiplication relative to the rate of spread of the infection. | the time-course of inhibition of tobacco mosaic virus synthesis in mechanically inoculated leaves by actinomycin d was not correlated with cell-to-cell spread of the infection, as previously shown to be the case with 2-thiouracil and guanidine inhibition. the amount of inhibition by actinomycin d declined after inoculation much more rapidly than the infection spread. an actinomycin-d-sensitive step or its product occurred in cells before they became infected. | 1978 | 621138 |
| estimation of the cell-to-cell spread of tobacco mosaic virus infection in mechanically inoculated leaves. | procedures to estimate cell-to-cell spread of tobacco mosaic virus (tmv) infection in mechanically inoculated leaves are presented mathematically and experimentally. based upon the mathematical estimation, it is demonstrated that resistance of tmv infection to 2-thiouracil and guanidine in mechanically inoculated leaves is correlated to the fraction of cells infected. the addition of 2-thiouracil or guanidine prevents cell-to-cell spread of tmv infection. | 1978 | 621139 |
| double-stranded rna is present in extracts of tobacco plants infected with tobacco mosaic virus. | serologically specific electron microscopy was used to detect double-stranded rna in extracts of tobacco infected with tobacco mosaic virus. assays were made immediately after extraction, without purification, concentration, or treatment with phenol or detergent. this indicates that the double-stranded rna is native and is not an artifact induced by purification methods. | 1978 | 622553 |
| the influence of protoplast concentration on retention of tobacco mosaic virus by tobacco protoplasts. | | 1978 | 622812 |
| location of tyrosine residues in the disk of tobacco-mosaic-virus protein and comparison of the subunit packing with that of the virus. | the products of iodination of the disk of tobacco mosaic virus coat protein have been investigated in order to locate the reactive amino acid residues in the three-dimensional structure. reaction occurs mainly with tyrosine-139 and, to a lesser extent, tyrosine-2 and the positions of these modified residues have been determined by x-ray crystallography. different extents of reaction are found in the two rings of the disk and also, on adding the high salt concentration needed for stabilisation of ... | 1978 | 631133 |
| the polarity of stripping of coat protein subunits from the rna in tobacco mosaic virus by dimethylsulphoxide. | | 1978 | 631329 |
| preparative immunoabsorption electrophoresis. | a method for separating a component from a mixture of antigens is described. the component which may be a virus or subfraction of a virus, is isolated by driving the mixture by electrophoresis through a gel containing precipitating antibodies directed against the unwanted components. the method is illustrated by the isolation of hepatitis b antigen from whole serum and by the separation of wild cucumber mosaic virus from a strain of tobacco mosaic virus. | 1978 | 631871 |
| [optical rotatory dispersion by 5 viruses of the tobacco mosaic virus group and their components]. | optical rotatory dispersion (ord) spectra in 250 to 350 nm region were measured for preparations of five tmv-like viruses (tmv vulgare, hr and u2 strains of tmv dolihosenation mosaic virus and cucumber virus 4) and also for rna and protein preparations of these viruses. the data obtained testify against the possibility that the double peak with maxima at 286 and 293 nm observed in ord of all the five viruses is due to interaction of tryptophan residues in virus coat protein with the rna of the v ... | 1978 | 634280 |
| leaky uag termination codon in tobacco mosaic virus rna. | | 1978 | 634374 |
| the interaction of sodium dodecyl sulfate with cucumber green mottle mosaic virus protein and tobacco mosaic virus protein. | | 1978 | 637561 |
| nucleotide sequence at the 5' extremity of tobacco-mosaic-virus rna. 1. the noncoding region (nucleotides 1-68). | the sequence of the 5' noncoding region of tobacco mosaic virus rna has been determined. the noncoding region is 68 nucleotides long and is unusual in that it contains no internal guanosine residues. the long t1 oligonucleotide containing the guanosine-free tract was isolated from a t1 ribonuclease digest of tobacco mosaic virus rna and sequenced by labelling techniques in vitro using polynucleotide kinase. the guanosine-free tract is terminated by the first potential initiation codon in the rna ... | 1978 | 639800 |
| nucleotide sequence at the 5' extremity of tobacco-mosaic-virus rna. 2. the coding region (nucleotides 69-236). | in the preceding paper it was shown that the first a-u-g codon in tobacco mosaic virus rna is separated from the 5' terminus by a sequence of 68 nucleotides devoid of internal guanosine residues. in this paper we present the sequence of 165 residues immediately following the first potential initiation codon. the characterized sequence contains four nonsense codons but none are in phase with the prospective initiation codon. several lines of evidence, including direct characterization of the port ... | 1978 | 639801 |
| isolation of viral double-stranded rnas using a licl fractionation procedure. | a general procedure for the isolation of virus-specific double-stranded rna (ds-rna) is discribed. the procedure is based on the differential solubility of different types of nucleic acids in licl. principal advantages over conventional methods are simplicity, avoidance of enzymatic treatment, and relatively good yields of undegraded ds-rna while permitting separation of several main groups of cellular and viral nucleic acids from the same batch of tissue. the method has been successfully applie ... | 1978 | 643823 |
| unusual segmental flexibility in a region of tobacco mosaic virus coat protein. | | 1978 | 661966 |
| the effect of tobacco mosaic virus infection on host and virus-specific protein synthesis in protoplasts. | | 1978 | 664212 |
| rna-dependent rna polymerases in uninfected and tobacco mosaic virus-infected tabacco leaves: viral induced stimulation of a host polymerase activity. | | 1978 | 664227 |
| a comparative study of the structure of tobacco mosaic virus and cucumber virus 4 by laser raman spectroscopy. | | 1978 | 666800 |
| an anomalous form of tobacco mosaic virus rna observed upon polyacrylamide gel electrophoresis. | | 1978 | 676078 |
| effect of supraoptimal temperatures upon tobacco mosaic virus rna replicase. | an examination of the effect of supraoptimal temperatures upon tobacco mosaic virus (tmv) rna replicase demonstrated that the enzyme activity rapidly decreased at 40 degrees whether incubated in vitro after extraction or in leaves at that temperature prior to extraction and assay. the loss of replicase activity paralled the decline in capacity for in vivo incorporation of 32p into tmv rna at 25 degrees following different periods of incubation at 40 degrews. the optimal temperature for replicase ... | 1978 | 681145 |
| migration of 40 s ribosomal subunits on messenger rna in the presence of edeine. | the antibiotic edenine induces binding of multiple 40 s ribosomes to reovirus messenger rnas, producing complexes that sediment rapidly in glycerol gradients. rapidly sedimenting complexes were also obtained with tobacco mosaic virus rna and rabbit globin mrna in the presence of edeine. following ribonuclease digestion of the heavy complexes, nuclease-resistant 32p-labeled reovirus fragments protected by 40 s ribosomes in the presence of edeine were recovered and fingerprinted. the sequence comp ... | 1978 | 681367 |
| [on the effect of some morpholinium compounds on the multiplication of rna viruses (author's transl)]. | in the present paper the authors report about the effect of n,n-dimethylmorpholinium chloride (dmmc), n,n-dimethyl-2-oxomorpholinium chloride (dmomc), and n,n-diethyl-2-oxomorpholinium chloride (deomc) on the multiplication of tobacco mosaic virus (tmv), potato virus x (pvx), and cucumber mosaic virus (cmv) as well as the plaque formation by the rna phages m 12, f 2, and qbeta. the used morpholinium compounds do not influence strikingly the multiplication of phytoviruses. the results found by se ... | 1978 | 685536 |
| electrostatic effects in proteins. | electrostatic effects dominate many aspects of protein behavior. when polypeptide chains fold up, most polar side chains seek the exterior, where they can be solvated. water bound in the interior has been found between the domains of enzymes of the chymotrypsin family, and between the subunits of hemoglobin and tobacco mosaic virus protein. assembly of this protein from disk to virus is triggered by electrostatic interactions between neighboring subunits. lysozyme stabilizes the constellation of ... | 1978 | 694508 |
| [conformational instability of viral rna and the specificity of the assembly of tobacco mosaic virus]. | | 1978 | 698317 |
| interaction of tobacco mosaic virus protein with synthetic polynucleotides containing a fluorescent label: optical properties of poly(a,epsilona) and poly(c,epsilonc) copolymers and energy migration from the tryptophan to 1,n6-ethenoadenine or 3,n4-ethenocytosine residues in rnp. | a spectrophotometric method for determination of the modification degrees and molar extinction coefficients for poly(a, epsilona) and poly(c, epsilonc) copolymers has been developed. dependence of some absorption and fluorescence parameters of the copolymers on the modification degree has been studied. distribution of modified residues in copolymers differs from random and depends on modification conditions. interaction between the tmv protein and copolymers has been investigated. the protein in ... | 1978 | 724512 |
| characterization of a temperature-sensitive mutant of tobacco mosaic virus deficient in synthesis of all rna species. | | 1978 | 726251 |
| characterization of a second protein associated with virions of tobacco mosaic virus. | | 1978 | 726261 |
| rotational decay constant of tobacco mosaic virus by depolarized photon-correlation spectroscopy. | | 1978 | 728547 |
| 5'-terminal 7-methylguanosine and mrna function. the effect of enzymatic decapping and of cap analogs on translation of tobacco-mosaic-virus rna and globin mrna in vitro. | 1. decapped tobacco mosaic virus (tmv) rna and rabbit globin mrna were prepared by enzymic treatment of rnas with nucleotide pyrophosphatase purified from potato. the extent of removal of 5'-terminal 7-methylguanosine 5'-monophosphate (m7gmp) from tmv rna was at least 97% as estimated by labeling of the 5' termini in vitro with s-adenosyl[methyl-3h]methionine catalysed by vaccinia virus methyltransferases. 2. the effect of enzymic decapping was compared with the effect of cap analogs on mrnas tr ... | 1978 | 729595 |
| quantized incorporation of rna during assembly of tobacco mosaic virus from protein disks. | | 1978 | 745247 |
| ion etching of tobacco mosaic virus and t4 bacteriophage. | a technique for the etching of biological materials by accelerated nitrogen ions is described. upon exposure to such a beam, tobacco mosaic virus becomes thinner in diameter and the head of t4 becomes smaller. experiments on radioactively labeled t4 suggest that the particle's protein is more susceptible to removal by the incident ions, and that the residual material is predominantly nucleic acid. | 1976 | 766845 |
| [mechanism of assembly of tobacco mosaic virus (author's transl)]. | | 1975 | 768392 |
| the development of the virus concept as reflected in corpora of studies on individual pathogenslessons of the plant viruses--tobacco mosaic virus. | | 1976 | 781420 |
| cell surface immunoglobulin. xii. localization of immunoglobulin on murine lymphocytes by scanning immunoelectromicroscopy. | the localization of immunoglobulin (ig) on the surface of murine splenic lymphocytes has been studied with the scanning electron microscope (sem), using a rabbit f(ab')2 hybrid antibody directed against mouse ig and tobacco mosaic virus (tmv) with tmv as a morphological marker. 35% of spleen cells and 3% of thymus cells were labeled by this method. labeled cells were usually small lymphocytes with moderate numbers of microvilli. the ig molecules were randomly distributed on the lymphocyte surfac ... | 1975 | 789089 |
| [a naturally occurring thermophilic strain of tobacco mosaic virus, isolated in madagascar]. | a strain of tobacco mosaic virus was isolated from tomato leaves grown in madagascar, in the region of tananarive. its optimal temperature for multiplication is between 30 and 34 degrees c, which makes it thermophilic compared to the common strain. this strain, however, does not appear to be identical to the thermoresistant tc strain. | 1975 | 807392 |
| [virus-free plants obtained by culture in vitro of anthers from tobacco plants infected by tobacco mosaic virus]. | | 1975 | 808293 |
| [cinnamic acid 4-hydroxylase and 0-methyltransferase activities in leaves of hypersensitive tobacco plants infected by tobacco mosaic virus]. | tobacco leaves of nicotiana tabacum l., variety samsun nn, show a low cinnamic acid-4-hydroxylase activity and a rather high o-methyltransferase activity. caffeic acid, the substrate of the latter enzyme, is methylated specifically in the meta position. in tmv-infected leaves a strong increase in the activities of phenylalanine ammonia-lyase and of these two enzymes occurs at the time of appearance and development of the necrotic reaction. | 1975 | 809178 |
| [tobacco mosaic virus and para-tobacco mosaic virus in cigarettes]. | | 1975 | 814472 |
| [interferonogenic and antiviral activity of the tobacco mosaic virus, tilorone and sodium nucleinate]. | production of endogenic interferon in animals in responce to administration of tobaco mozaic virus, tilorone and sodium nucleinate was shown. dependence of interferon production on the type of the inductor and the route of its administration was studied. absolute innocuiuty of the tobaco mozaic virus for monkeys (macaco-resus) and mice, as well as the absence of any side effects in humans treated with it perorally was shown. the tobaco mozaic virus, tilorone and sodium nucleinate used perorally ... | 1976 | 818948 |
| [potent natural inhibitors of tobacco mosaic virus multiplication]. | the hypersensitivity reaction in nicotiana tabacum var. xanthi n.c. infected with tobacco mosaic virus (t.m.v.) leads to the production of aromatic amides (ferulylputrescine, diferulylputrescine, p-coumarylputrescine and di-p-coumarylputrescine) inthe cells around the necroses. similar compounds are formed in xanthi plants after floral induction. p-coumarylputrescine, di-p-coumarylputrescine and caffeylputrescine strongly inhibit t.m.v. multiplication. | 1976 | 822960 |
| an efficient mrna-dependent translation system from reticulocyte lysates. | a simple method is described for converting a standard rabbit reticulocyte cell-free extract (lysate) into an mrna-dependent protein synthesis system. the lysate is preincubated with cacl2 and micrococcal nuclease, and then excess ethyleneglycol-bis(2-aminoethylether)-n,n'-tetraacetic acid is added to chelate the ca2+ and inactivate the nuclease. lysates treated in this way have neglibible endogenous amino acid incorporation activity, but 75% of the activity of the original lysate can be recover ... | 1976 | 823012 |
| formation of an 80 s methionyl-trna initiation complex with soluble factors from wheat germ. | wheat germ supernatant is resolved into four fractions, c3alpha, c3beta, d and a, all of which are required in addition to elongation ef1 and ef2 for tobacco mosaic virus-rna (tmv-rna)-catalyzed amino acid polymerization. fractions c3beta and d function in the binding of met-trnaimet to the 40 s ribosomal subunit forming an unstable 40 s-met-trna1met complex that is detected in sucrose gradients only after fixation with glutaraldehyde. fraction c3beta binds met-trnaimet suggesting that a c3beta- ... | 1976 | 823157 |
| location of the origin for viral reassembly on tobacco mosaic virus rna and its relation to stable fragment. | | 1976 | 826409 |
| in vitro replication of tobacco mosaic virus rna in tobacco callus cultures: solubilization of membrane-bound replicase and partial purification. | a fraction containing membrane-bound tobacco mosaic virus rna replicase was isolated form tobacco mosaic virus-infected tobacco callus cultures. the replicase activity reached a maximum 60 h after inoculation and then declined. the enzyme activity was insensitive to actinomycin d and dnase. the corresponding fraction from healthy callus contained essentially no activity. the viral rna synthesis in vitro proceeded linearly for 30 min and required the four nucleotide triphosphates and mg2+ ions. m ... | 1977 | 833935 |
| configuration of tobacco mosaic virus, rna during virus assembly. | when tmv reassembles, the uncoated rna is folded back along the growing rod, probably down the central hole. this surprising configuration is essential for rapid elongation--presumably supplying rna to its site of incorporation while keeping the bulk of the free rna out of the way. | 1977 | 834264 |
| the role of the epidermis in local lesion formation and the multiplication of tobacco mosaic virus and its relation to kinetin. | | 1977 | 835224 |
| cross protection between mild and severe strains of tobacco mosaic virus in doubly inoculated tomato plants. | | 1977 | 860401 |
| structure of rna and rna binding site in tobacco mosaic virus from 4-a map calculated from x-ray fibre diagrams. | the structure of tobacco mosaic virus has been solved to a resolution of 4 a using fibre diffraction methods. the general fold of the protein is clear, the conformation may be deduced in many places, and the conformation of the rna and its interaction with the protein can be seen. | 1977 | 865612 |
| location of the initiation site on tobacco mosaic virus rna involved in assembly of the virus in vitro. | | 1977 | 867810 |
| the identification of mild and severe strains of tobacco mosaic virus in doubly inoculated tomato plants. | using specific antisera, it was possible to identify mild (mii-i6) and the o and i strains of tobacco mosaic virus (tmv) in tomato plants infected with the mild strain following challenge inoculation with the wild strains. in addition, with single lesion isolates of the mii-i6 and o strains it has been shown that both strains can be assayed simultaneously in mixed isolates on nicotiana glutinisa based on differences in local lesion size. the accuracy of this differential local lesion assay was c ... | 1977 | 869306 |
| further studies on the biology of an antiviral factor (avf) from virus-infected plants and its association with the n-gene of nicotiana species. | the antiviral factor (avf) from virus-infected plants, purified on polyacrylamide gels, could be labelled with radioactive phosphorus and its activity could be eluted from the gels. the radioactivity and the antiviral activity were co-purified and thus co-electrophoresed; hence, the previously reported radioactive zone (antignus, sela & harpaz, 1975) can be regarded as avf. the production of avf requires both the presence of the n-gene in the plant as well as virus infection. avf production is i ... | 1977 | 870617 |
| high-pressure chromatography of tobacco mosaic virus on spheron gels. | high-pressure chromatography on spheron 300 btd and spheron 1000 was used to separate tobacco mosaic virus from other components of an extract from infected tobacco leaves, including f1, a protein having a molecular weight of 0.58 x 10(6). comparative experiments have shown that the virus is not adsorbed by the carrier and is eluted in the free volume of the column; the virus is free from contamination by vegetable-juice components and is regained quantitatively. the virus can be separated parti ... | 1977 | 874027 |
| leader sequence of 71 nucleotides devoid of g in tobacco mosaic virus rna. | | 1977 | 876375 |
| the isolation of tobacco mosaic virus rna fragments containing the origin for viral assembly. | | 1977 | 884731 |
| the nucleotide sequence at the origin for assembly on tobacco mosaic virus rna. | | 1977 | 884732 |
| sequence from the assembly nucleation region of tmv rna. | in an effort to isolate rna sequences containing the assembly nucleation region, uniformly 32p-labeled tobacco mosaic virus rna was partially digested with pancreatic ribonuclease, and the mixture of fragments was incubated with limited amounts of tobacco mosaic virus protein disks in conditions favorable for reconstitution. the rna fragments which became encapsidated were purified and sequenced by conventional techniques. the sequence of the first 139 nucleotides of p1, the principal encapsidat ... | 1977 | 884733 |
| characterization and in vitro translation of the rnas from less-than-full-length, virus-related, nucleoprotein rods present in tobacco mosaic virus preparations. | | 1977 | 888358 |
| photoreactivation of dna-containing cauliflower mosaic virus and tobacco mosaic virus rna on datura. | | 1977 | 905348 |
| fate of tobacco mosaic virus after entering the host cell. iii. partial uncoating. | diminutive viral rnas recovered from tobacco leaves inoculated with 32p-tmv were investigated. at 3.5 hr after inoculation, most of the viral rna without coat protein revealed two peaks after sucrose density gradient analysis of sds-extract from 12,000 x g leaf pellet. the first peak appeared between bacterial ribosomal rna of 16 s and 5 s and the second peak was around 5 s. these two peaks were digestible with rnase and they appeared as early as 5 min after inoculation. these diminutive rnas se ... | 1977 | 909456 |
| isolation of viruses by electro-extraction of infected plants. | the isolation of viruses from infected plant material by a process termed electro-extraction appeared to be a convenient and simple method of obtaining viruses in a fair state of purity. the method has the advantage over the conventional methods of virus purification that the infected plant tissue is not disintegrated and that organic solvents such as chloroform and butanol are avoided. the procedure used was demonstrated on the extraction of tobacco mosaic virus (tmv) from infected tobacco and ... | 1977 | 909872 |
| [virus and plant-host interrelationships in the presence of rifamycin]. | the effect of rifamycine on the infectivity and accumulation in tissues of potato x-virus (pxv) and tobacco mosaic virus (tmv) in local and systemic infections of plants was studied. high concentrations of rifamycine (50--100 microgram/ml) were found to inhibit and low (2 microgram/ml) to stimulate the infectivity of the viruses. rifamycine reduced accumulation of pxv in isolated leaves of thornapple and growth of necrotic lesions caused by tmv on tobacco leaves. the effectiveness of the antibio ... | 1977 | 930055 |
| the relationship between soil-borne wheat mosaic virus and tobacco mosaic virus. | | 1976 | 936469 |
| in vitro and in vivo translation of the ribonucleic acids of a cowpea strain of tobacco mosaic virus. | | 1976 | 936472 |
| structure and immunogenic behaviour of methylated tobacco mosaic virus. | tobacco mosaic virus was methylated, using various concentrations of dimethylsulfate. the methylated virus sample with still intact particles was subjected to sequential analysis. the sites and the degree of methylation were determined in the tryptic peptides. tyrosine 139 and cysteine 27 are more accessible to methylation than tyrosine 72, lysine 68 and tyrosine 2. a limited number of carboxyl groups was also methylated. the ability of methylated and original tobacco mosaic virus to initiate th ... | 1976 | 944592 |
| [action of some polysaccharide containing complexes of microbial origin on the tobacco mosaic virus and on the influenza virus]. | | 1976 | 948273 |
| immunochemical studies of tobacco mosaic virus--ii. univalent and monogamous bivalent binding of igg antibody. | | 1976 | 950245 |
| a messenger rna for capsid protein isolated from tobacco mosaic virus-infected tissue. | | 1976 | 960569 |
| a study of nucleotide sequence homology between strains of tobacco mosaic virus. | | 1976 | 960571 |
| changes in soluble proteins in callus cells of hypersensitive tobacco inoculated with tobacco mosaic virus. | protein changes occurred in callus cells of hypersensitive tobacco (nicotiana tabacum var. xanthi-nc) 72 hr after inoculation with tobacco mosaic virus and incubation on a minimal growth medium. two protein bands, serologically related to viral coat protein, were obtained from extracts of infected cells following electrophoresis on 7% and 10% polyacrylamide gels. an additional, slower migrating protein, perhaps due to virus-induced stimulation of a host protein, also was detected. although local ... | 1976 | 965017 |
| [action of gamma irradiation on the infectivity of the potato virus x, the tmv and their rnas]. | | 1976 | 967058 |
| the hand of the stacked-disk aggregate of tobacco mosaic virus protein. | | 1976 | 972391 |
| interaction of elongation factor 1 with aminoacylated brome mosaic virus and trna's. | tyrosylated brome mosaic virus rna was found to interact with a binary complex of wheat germ, elongation factor 1 and [3h]gtp. increasing amounts of the aminoacylated viral rna proportionately reduced radioactivity bound to a nitrocellulose filter, as has previously been noted by others for the charged forms of tobacco mosaic virus, turnip yellow mosaic virus, and trna's. however, sephadex chromatography of the products showed that instead of forming the ternary complex elongation factor-gtp-ami ... | 1976 | 978788 |
| the folding of protein chains. prediction of tobacco mosaic virus protein tertiary structure. | | 1976 | 992043 |
| in vitro translation of polyribosome-associated rnas from tobamovirus-infected plants. | rnas associated with polyribosomes in plants infected with the u2 strain of tobacco mosaic virus (tmv) or with sunnhemp mosaic virus have been isolated. most are about 0.35 x 10(6) daltons in weight. they translate efficiently in vitro to produce their respective coat proteins which were identified by their serological behavior and peptide composition. they also reassemble in vitro with coat protein. the coat protein of sunnhemp mosaic virus reassembles more quickly than that of tmv u2. | 1976 | 1010713 |
| translation in vitro of artificially produced fragments of a tobamovirus genome. | particles of the u2 strain of tobacco mosaic virus (tmv) were partly disassembled by sds, treated with rnases and then phenol, and yielded rna molecules one quarter to half the size of the intact virus genome. these molecules, when translated in vitro, produced the coat protein of the virus. reassembly experiments indicated that the active messenger molecules were those that most rapidly reassembled with coat protein; the rate of reassembly was greatly diminished by treatment with spleen phospho ... | 1976 | 1010714 |
| on the nature of the difference in the densities of the particles of two tobamoviruses. | particles of sunnhemp mosaic virus (shmv) are denser than those of the u2 strain of tobacco mosaic virus (t2mv) when their densities are estimated by equilibrium centrifugation in gradients of either cesium chloride or metrizamide; in cesium chloride the densities are 1.318 and 1.307 g/ml, and in metrizamide they are 1.249 and 1.240 g/ml. experiments with particles reassembled from homologous or heterologous mixtures of the rnas and coat proteins of the viruses show that the difference in their ... | 1976 | 1010716 |
| [purification of pvy, pvx, tmv and spherical virus of pinks using polyethylene glycol and gel filtration]. | | 1976 | 1012092 |
| homogenization-resistant and -susceptible components of tobacco mosaic virus replicative form rna. | when prepared from tissue frozen with liquid nitrogen, tobacco mosaic virus replicative form rna (tmv rf) was uniform in size but when prepared by high-speed homogenization, or when tmv rf prepared with liquid nitrogen was homogenized, 80 to 90% of the rf broke into relatively discrete pieces. the unbroken rf was not fragmented by additional homogenization. the tmv rf components susceptible and resistant to breakage, respectively, were synthesized with similar kinetics in relation to length of l ... | 1976 | 1018172 |
| a tobacco mosaic virus mutant with non-functional coat protein and its revertant: relationship to the virus assembly process. | | 1976 | 1018325 |
| binding of tobacco mosaic virus to membrane material isolated from tobacco leaves. | binding of tobacco mosaic virus (tmv) to disrupted tobacco leaf membrane was studied. membrane isolated from tobacco leaves was treated successively with (nh4)2so4, li-diiodosalicylate and then pronase. tmv-binding substance was thus isolated in a soluble form. from enzymatic digestion experiments, it was suggested that the binding substance was composed of lipid and carbohydrate. | 1976 | 1018345 |
| sequence of 71 nucleotides at the 3'-end of tobacco mosaic virus rna. | the sequence of the first 71 nucleotides from the 3'-oh end of tobacco mosaic virus rna has been determined. after total t1 ribonuclease digestion of the viral rna, the oligonucleotide c-c-c-a-oh, which originates from the 3'-oh terminus of the rna, may be readily detected by electrophoresis at ph 2.5 or ph 3.0; it is the only oligonucleotide that migrates toward the cathode at these phs. this property has been used to screen the purified products of partial t1 ribonuclease digestion of tobacco ... | 1975 | 1055385 |
| inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate. | translation of rabbit globin mrna in a wheat germ protein-synthesizing system is inhibited by the nucleotide 7-methylguanosine-5'-monophosphate (m7g5'p) but not by other guanosine nucleotides without the 7-methyl group or with the phosphate in a different position. translation of rna of tobacco mosaic virus and poly(a) + hela rna is also inhibited by m7g5'p. we show that m7g5'p prevents the association of mrna with ribosomal subunits to form an initiation complex. we propose that m7g5'p interact ... | 1976 | 1061116 |
| biological consequences of incorporation of 5-fluorocytidine in the rna of 5-fluorouracil-treated eukaryotic cells. | treatment of hela cells with 5-fluoro-[3h]uracil leads to the incorporation into cellular rna of 5-fluorocytidine to the extent of about 0.2% of the 5-fluorouridine incorporated. in tobacco mosaic virus rna produced in tobacco leaves this ratio is one order of magnitude lower. copolymers of cytidylic with 5-fluorocytidylic acids show unchanged template activity with e. coli rna polymerase, but slightly altered messenger activity in the wheat germ system, compared to poly(c), and it is suggested ... | 1976 | 1064021 |
| initiation of translation directed by 42s and 26s rnas from semliki forest virus in vitro. | the proteins synthesized in vitro in response to 42s and 26s rnas from semliki forest virus were labeled with formyl-[35s]methionine from initiator trna. one protein which comigrated with viral capsid protein was labeled under the direction of 26s rna, and only one labeled peptide was detected after digestion with trypsin. further digestion with pronase gave rise to the dipeptide fmet-asn. several labeled polypeptides were found in the 42s rna directed product and these had molecular weights of ... | 1976 | 1067601 |
| immunospecific labeling of mouse lymphocytes in the scanning electron microscope. | bone marrow-derived (b) and thymus-derived (t) balb/c mouse lymphocytes were identified in the scanning electron microscope (sem) by the immunospecific attachment of one of several kinds of large-molecular-weight markers distinguishable in sem. these markers (tobacco mosaic virus, keyhole limpet hemocyanin, bushy stunt virus, and bacteriophage t4) could be modified with hapten groups and linked with anti-hapten antibody, in an indirect (sandwich) scheme, to hapten-modified anti-cell-surface anti ... | 1976 | 1087366 |
| scanning electron microscopy of tobacco mosaic virus-labeled lymphocyte surface antigens. | the study of surface antigen by immunoelectron microscopy has been hampered by the fact that thin sections of cells provide only a view of the cell perimeter in an essentially two- dimensional fashion. although the reconstruction of the entire cell from serial sections has been accomplished (1), it remains too exacting a technique and will find only exceptional application. carbon-platinum replicas (2) allow the inspection of larger surface areas and therefore are better suited for studying the ... | 1975 | 1089747 |
| mechanism of assembly of tobacco mosaic virus in vitro. | we previously proposed that the assembly reaction of tmv in vitro is initiated by 20s protein aggregate specifically interacting to the 5'-end of tmv-rna, after which the helical rod grows by the addition of protein subunits. other workers have reported that the source of protein for the growing helix is also the 20s protein aggregate, but not protein subunits. we now summarize the experimental results that confirm our previous hypothesis. 1) tmv-particle, as gauged by infectivity assay and sucr ... | 1975 | 1101655 |
| rates of rod accumulation and viral rna synthesis during early and late stages of tobacco mosaic virus infection in young, expanding tobacco leaves. | | 1975 | 1111207 |
| a kinetic model of tmv-rna replication based on rates of virus accumulation in vivo. | | 1975 | 1111208 |
| replication of tobacco mosiac virus, vi replicative intermediate and tmv-rna-related rnas associated with polyribosomes. | | 1975 | 1111217 |
| structure of tobacco mosaic virus at 6.7 å resolution. | the electron density distribution of tobacco mosaic virus has been determined to 6.7 a resolution by analysis of the x-ray diffraction pattern given by oriented gels of the virus. this has been achieved by separation of overlapping bessel function terms by a technique analogous to crystallographic isomorphous replacement. the course of the polypeptide chain of the coat protein may be traced for a large part of its length. | 1975 | 1113882 |
| retention and dissociation of tobacco mosaic virus by tobacco protoplasts. | | 1975 | 1114698 |
| protein synthesis in tobacco protoplasts infected with tobacco mosaic virus. | | 1975 | 1114703 |
| the effect of cations on the alkaline dissociation of tobacco mosaic virus. | | 1975 | 1114714 |
| protein composition of unusual tobacco mosaic virus strains. | amino acid analyses have been made of the proteins of single-lesion isolates of five strains of tobacco mosaic virus (tmv) differentiated by lycopersicon hosts. these hosts differed in their genetical control of resistance to tmv, and the virus strains had therefore survived specific selection pressures. two of the five strains differed in their amino acid composition from type tmv and from all other tomato strains of tmv previously examined. symptoms induced by the five strains in four tomato l ... | 1975 | 1115413 |
| the uncoating of native and reconstituted tmv by dimethylsulphoxide: the polarity of stripping. | the effect of increasing concentrations of dimethylsulphoxide (dmso) on the stability of native and reconstituted tobacco mosaic virus (tmv) has been investigated by means of electron microscopy and infectivity assay. the removal of protein subunits from tmv by dmso was found to be a stepwise process. subunits are first removed from the 3'oh end of the rod but at about 72% dmso the site at which uncoating occurs shifts to the 5' end. studies with reconstituted and partially reconstituted tmv con ... | 1975 | 1120937 |
| thermally induced conformational changes of tobacco mosaic virus and their protein assemblies. | the measurement of the fluorescence intensity of tryptophan residues showed that a reversible transition in the local structure took place between 20 degrees c and 30c in intact virus particles and reconstituted protein assemblies of the ordinary strain and the tomato strain of tobacco mosaic virus. during this transition the overall polymer structure was maintained. in the case of the bean strain of tobacco mosaic virus, however, the fluorescence intensity did not show any transition in the sam ... | 1975 | 1125268 |