| complete genome sequence of nine isolates of canna yellow streak virus reveals its relationship to the sugarcane mosaic virus (scmv) subgroup of potyviruses. | complete genome sequences were obtained from nine isolates of canna yellow streak virus (caysv). caysv belongs to the sugarcane mosaic virus (scmv) subgroup of potyviruses with johnsongrass mosaic virus (jgmv) as its closest relative. multiple sequence alignments showed a pattern of amino acid substitutions in the cp sequences, which enabled us to relate these isolates to south east asian or european isolates. biological characterization of caysv identified nicotiana benthamiana, chenopodium qui ... | 2015 | 25567205 |
| further characterization of maize chlorotic mottle virus and its synergistic interaction with sugarcane mosaic virus in maize. | maize chlorotic mottle virus (mcmv) was first reported in maize in china in 2009. in this study we further analyzed the epidemiology of mcmv and corn lethal necrosis disease (clnd) in china. we determined that clnd observed in china was caused by co-infection of mcmv and sugarcane mosaic virus (scmv). phylogenetic analysis using four full-length mcmv cdna sequences obtained in this study and the available mcmv sequences retrieved from genbank indicated that chinese mcmv isolates were derived fro ... | 2017 | 28059116 |
| synergistic infection of two viruses mcmv and scmv increases the accumulations of both mcmv and mcmv-derived sirnas in maize. | the co-infection of maize chlorotic mottle virus (mcmv) and sugarcane mosaic virus (scmv) can cause maize lethal necrosis. however, the mechanism underlying the synergistic interaction between these two viruses remains elusive. in this study, we found that the co-infection of mcmv and scmv increased the accumulation of mcmv. moreover, the profiles of virus-derived sirnas (vsirnas) from mcmv and scmv in single- and co-infected maize plants were obtained by high-throughput sequencing. our data sho ... | 2016 | 26864602 |
| genome-wide association and genomic prediction of resistance to maize lethal necrosis disease in tropical maize germplasm. | genome-wide association analysis in tropical and subtropical maize germplasm revealed that mlnd resistance is influenced by multiple genomic regions with small to medium effects. the maize lethal necrosis disease (mlnd) caused by synergistic interaction of maize chlorotic mottle virus and sugarcane mosaic virus, and has emerged as a serious threat to maize production in eastern africa since 2011. our objective was to gain insights into the genetic architecture underlying the resistance to mlnd b ... | 2015 | 26152570 |
| comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus. | sugarcane mosaic virus (scmv) is an important viral pathogen and has caused serious losses in grain and forage yield. to identify candidate scmv resistance proteins and to explore the molecular mechanisms involved in the plant-scmv interaction, we conducted proteomic analyses of leaf samples from resistant and susceptible ecotypes of maize infected with scmv. proteins were analyzed by quantitative two-dimensional differential gel electrophoresis (2d-dige), and 93 protein spots showed statistical ... | 2013 | 23770298 |
| genetic diversity and population structure of sugarcane mosaic virus. | sugarcane mosaic virus (scmv) is one of the causal pathogens of mosaic diseases on sugarcane, maize, sorghum and some other graminaceous species with worldwide distribution. the global genetic diversity and molecular evolution of scmv capsid protein (cp) gene were investigated with the nucleotide sequences available in the genbank database. phylogenetic analyses revealed that scmv isolates clustered in relation to their original hosts, and geographically distinct isolates from maize or sugarcane ... | 2013 | 23116592 |
| a one-step real-time rt-pcr assay for the detection and quantitation of sugarcane streak mosaic virus. | sugarcane mosaic disease is caused by the sugarcane streak mosaic virus (scsmv; genus poacevirus, family potyviridae) which is common in some asian countries. here, we established a protocol of a one-step real-time quantitative reverse transcription pcr (real-time qrt-pcr) using the taqman probe for the detection of scsmv in sugarcane. primers and probes were designed within the conserved region of the scsmv coat protein (cp) gene sequences. standard single-stranded rna (ssrna) generated by pcr- ... | 2015 | 26185758 |
| transgenic sugarcane resistant to sorghum mosaic virus based on coat protein gene silencing by rna interference. | as one of the critical diseases of sugarcane, sugarcane mosaic disease can lead to serious decline in stalk yield and sucrose content. it is mainly caused by potyvirus sugarcane mosaic virus (scmv) and/or sorghum mosaic virus (srmv), with additional differences in viral strains. rna interference (rnai) is a novel strategy for producing viral resistant plants. in this study, based on multiple sequence alignment conducted on genomic sequences of different strains and isolates of srmv, the conserve ... | 2015 | 25685813 |
| development of a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay for the detection of sugarcane mosaic virus and sorghum mosaic virus in sugarcane. | a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for detecting sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) in sugarcane. six sets of four primers corresponding to the conserved coat protein gene were designed and tested for each virus. three primer sets designed for detecting scmv and four for detecting srmv were successful in the rt-lamp assay. the effective primer sets were not only specific for their target virus, but also able to ... | 2015 | 25445794 |
| expression analysis of hairpin rna carrying sugarcane mosaic virus (scmv) derived sequences and transgenic resistance development in a model rice plant. | developing transgenic resistance in monocotyledonous crops against pathogens remains a challenging area of research. sugarcane mosaic virus (scmv) is a serious pathogen of many monocotyledonous crops including sugarcane. the objective of present study was to analyze transgenic expression of hairpin rna (hprna), targeting simultaneously cp (coat protein) and hc-pro (helper component-proteinase) genes of scmv, in a model rice plant. conserved nucleotide sequences, exclusive for dag (aspartic acid- ... | 2017 | 28255554 |
| field performance of transgenic sugarcane lines resistant to sugarcane mosaic virus. | sugarcane mosaic disease is mainly caused by the sugarcane mosaic virus (scmv), which can significantly reduce stalk yield and sucrose content of sugarcane in the field. coat protein mediated protection (cpmp) is an effective strategy to improve virus resistance. a 2-year field study was conducted to compare five independent transgenic sugarcane lines carrying the scmv-cp gene (i.e., b2, b36, b38, b48, and b51) with the wild-type parental clone badila (wt). agronomic performance, resistance to s ... | 2017 | 28228765 |
| an atypical thioredoxin imparts early resistance to sugarcane mosaic virus in maize. | sugarcane mosaic virus (scmv) causes substantial losses of grain yield and forage biomass in susceptible maize worldwide. a major quantitative trait locus, scmv1, has been identified to impart strong resistance to scmv at the early infection stage. here, we demonstrate that zmtrxh, encoding an atypical h-type thioredoxin, is the causal gene at scmv1, and that its transcript abundance correlated strongly with maize resistance to scmv. zmtrxh alleles, whether they are resistant or susceptible, sha ... | 2017 | 28216424 |
| iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences. | iranian johnsongrass mosaic virus (ijmv) is one of the most prevalent viruses causing maize mosaic disease in iran. an ijmv isolate, maz-bah, was obtained from the maize showing mosaic symptoms in mazandaran, north of iran. the complete genomic sequence of maz-bah is 9544 nucleotides, excluding the poly(a) tail. it contains one single open reading frame of 9165 nucleotides and encodes a large polyprotein of 3054 amino acids, flanked by a 5'-untranslated region (utr) of 143 nucleotides and a 3'-u ... | 2017 | 27632283 |
| molecular variability and distribution of sugarcane mosaic virus in shanxi, china. | sugarcane mosaic virus (scmv) is responsible for large-scale economic losses in the global production of sugarcane, maize, sorghum, and some other graminaceous species. to understand the evolutionary mechanism of scmv populations, this virus was studied in shanxi, china. a total of 86 maize leaf samples (41 samples in 2012 and 45 samples in 2013) were collected from 4 regions of shanxi. | 2016 | 26987060 |
| the complete genome sequences of two naturally occurring recombinant isolates of sugarcane mosaic virus from iran. | sugarcane mosaic virus (scmv) is the most prevalent virus causing sugarcane mosaic and maize dwarf mosaic diseases. here, we presented the first two complete genomic sequences of iranian scmv isolates, nra and zra from sugarcane and maize. the complete genome sequences of nra and zra were, respectively, 9571 and 9572 nucleotides (nt) in length, excluding the 3'-terminal poly(a) tail. both isolates contained a 5'-untranslated region (utr) of 149 nt, an open reading frame of 9192 nt encoding a pol ... | 2016 | 26905544 |
| characterization of sugarcane mosaic virus scmv1 and scmv2 resistance regions by regional association analysis in maize. | sugarcane mosaic virus (scmv) causes one of the most severe virus diseases in maize worldwide, resulting in reduced grain and forage yield in susceptible cultivars. in this study, two association panels consisting of 94 inbred lines each, from china and the u.s., were characterized for resistance to two isolates: scmv-seehausen and scmv-bj. the population structure of both association panels was analyzed using 3072 single nucleotide polymorphism (snp) markers. the chinese and the u.s. panel were ... | 2015 | 26488483 |
| sugarcane elongin c is involved in infection by sugarcane mosaic disease pathogens. | sugarcane (saccharum sp. hybrid) provides the main source of sugar for humans. sugarcane mosaic disease (smd) is a major threat to sugarcane production. currently, control of smd is mainly dependent on breeding resistant cultivars through hybridization, which is time-consuming. understanding the mechanism of viral infection may facilitate novel strategies to breed cultivars resistant to smd and to control the disease. in this study, a wide interaction was detected between the viral vpg protein a ... | 2015 | 26362180 |
| phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus. | protein phosphorylation plays a pivotal role in the regulation of many cellular events. no information is yet available, however, on protein phosphorylation in plants in response to virus infection. in this study, we characterized phosphoproteomes of resistant and susceptible genotypes of maize (zea mays l.) in response to sugarcane mosaic virus (scmv) infection. based on isotope tags for relative and absolute quantification technology, tio2 enrichment method and lc-ms/ms analysis, we identified ... | 2015 | 25488425 |
| characterization of small interfering rnas derived from sugarcane mosaic virus in infected maize plants by deep sequencing. | rna silencing is a conserved surveillance mechanism against viruses in plants. it is mediated by dicer-like (dcl) proteins producing small interfering rnas (sirnas), which guide specific argonaute (ago)-containing complexes to inactivate viral genomes and may promote the silencing of host mrnas. in this study, we obtained the profile of virus-derived sirnas (vsirnas) from sugarcane mosaic virus (scmv) in infected maize (zea mays l.) plants by deep sequencing. our data showed that vsirnas which d ... | 2014 | 24819114 |
| combined linkage and association mapping reveals candidates for scmv1, a major locus involved in resistance to sugarcane mosaic virus (scmv) in maize. | sugarcane mosaic virus (scmv) disease causes substantial losses of grain yield and forage biomass in susceptible maize cultivars. maize resistance to scmv is associated with two dominant genes, scmv1 and scmv2, which are located on the short arm of chromosome 6 and near the centromere region of chromosome 3, respectively. we combined both linkage and association mapping to identify positional candidate genes for scmv1. | 2013 | 24134222 |
| proteomic and phytohormone analysis of the response of maize (zea mays l.) seedlings to sugarcane mosaic virus. | sugarcane mosaic virus (scmv) is an important virus pathogen in crop production, causing serious losses in grain and forage yields in susceptible cultivars. control strategies have been developed, but only marginal successes have been achieved. for the efficient control of this virus, a better understanding of its interactions and associated resistance mechanisms at the molecular level is required. | 2013 | 23894637 |
| identification of differentially regulated maize proteins conditioning sugarcane mosaic virus systemic infection. | sugarcane mosaic virus (scmv) is the most important cause of maize dwarf mosaic disease. to identify maize genes responsive to scmv infection and that may be involved in pathogenesis, a comparative proteomic analysis was performed using the first and second systemically infected leaves (termed 1 sl and 2 sl, respectively). seventy-one differentially expressed proteins were identified in 1 sl and 2 sl upon scmv infection. among them, eight proteins showed the same changing patterns in both 1 sl a ... | 2017 | 28627019 |
| modeling virus coinfection to inform management of maize lethal necrosis in kenya. | maize lethal necrosis (mln) has emerged as a serious threat to food security in sub-saharan africa. mln is caused by coinfection with two viruses, maize chlorotic mottle virus and a potyvirus, often sugarcane mosaic virus. to better understand the dynamics of mln and to provide insight into disease management, we modeled the spread of the viruses causing mln within and between growing seasons. the model allows for transmission via vectors, soil, and seed, as well as exogenous sources of infectio ... | 2017 | 28535127 |
| dissecting the molecular mechanism of the subcellular localization and cell-to-cell movement of the sugarcane mosaic virus p3n-pipo. | the coding sequence of p3n-pipo was cloned by fusion pcr from sugarcane mosaic virus (scmv), a main causal agent of sugarcane (saccharum spp. hybrid) mosaic disease. scmv p3n-pipo preferentially localized to the plasma membrane (pm) compared with the plasmodesmata (pd), as demonstrated by transient expression and plasmolysis assays in the leaf epidermal cells of nicotiana benthamiana. the subcellular localization of the p3n-pipo mutants p3n-pipot1 and p3n-pipot2 with 29 and 63 amino acids delete ... | 2017 | 28852157 |
| auxin binding protein 1 reinforces resistance to sugarcane mosaic virus in maize. | | 2017 | 28827193 |
| occurrence and evolutionary analysis of coat protein gene sequences of iranian isolates of sugarcane mosaic virus. | sugarcane mosaic virus (scmv) is one of the most damaging viruses infecting sugarcane, maize and some other graminaceous species around the world. to investigate the genetic diversity of scmv in iran, the coat protein (cp) gene sequences of 23 scmv isolates from different hosts were determined. the nucleotide sequence identity among iranian isolates was more than 96%. they shared nucleotide identities of 75.5-99.9% with those of other scmv isolates available in genbank, the highest with the egyp ... | 2017 | 28592948 |