| cytotoxic immune response of puppies to feline sarcoma virus induced tumors. | the cytotoxic immune response of puppies to feline sarcoma virus induced tumors was studied. neonatal puppies were compared with adolescent dogs. three different types of cytotoxicity were investigated: complement dependent cytotoxicity, t-cell-mediated cytotoxicity and antibody dependent cellular cytotoxicity. the relationship between the spontaneous regression of the sarcoma and the development of the immune system of the puppies is discussed. | 1984 | 6093343 |
| detection and cloning of new htlv-related endogenous sequences in man. | human t-cell leukemia virus (htlv) type i-related endogenous sequences (hres) have been cloned from a human genomic library. hres-1/1 is present in dna of all normal donors examined. by nucleotide sequence analysis, hres-1/1 contains two potential open reading frames capable of encoding a p25 and a p15. a 684 bp flanking region 5' from the first atg codon of p25 contains a tata-box, a poly-adenylation signal, a putative trna primer binding site, and inverted repeats at locations which are typica ... | 1989 | 2780312 |
| differences in mechanisms of transformation by independent feline sarcoma virus isolates. | the gardner and snyder-theilen isolates of feline sarcoma virus (fesv) have previously been shown to encode high-molecular-weight polyproteins with a transforming function and an associated tyrosine-specific protein kinase activity. cells transformed by these viruses exhibited morphological alterations, elevated levels of phosphotyrosine, and a reduced capacity for binding epidermal growth factor. in addition, polyproteins encoded by both of these fesv isolates bound to, and phosphorylated tyros ... | 1981 | 6165838 |
| origin and biological properties of a new feline sarcoma virus. | | 1982 | 6175084 |
| structure and origins of the hz2-feline sarcoma virus. | the hz2-feline sarcoma virus (hz2-fesv) is a replication-defective acute transforming feline retrovirus with oncogene homology to abelson murine leukemia virus (a-mulv) (p. besmer, w.d. hardy,jr., e. e. zuckerman, p. j. bergold, l. lederman, and h. w. snyder, jr. (1983) nature (london) 303, 825-828). in contrast to a-mulv which was isolated from a hematopoietic tumor, the hz2-fesv derives from a multicentric fibrosarcoma. we have molecularly cloned the hz2-fesv provirus from mink hz2-fesv nonpro ... | 1987 | 2884777 |
| mouse retroviral sequences acquired by cell lines after passaging through nude mice detected by hybridization of the fms probe psm3. | the expression of a large rna transcript, 8.5 to 9.5 kilobases, possibly related to the fms oncogene in mouse, rat, and human tumor cells, has been described in the literature. however, the psm3 fms probe used to detect this gene transcript contains a significant amount of the pol gene of the susan mcdonough strain of feline sarcoma virus from which it was derived. using a fms probe which does not contain any viral pol sequences, no such "fms-related" transcripts were detected in cell lines prev ... | 1989 | 2910482 |
| preparation of rat monoclonal antibodies to epitopes encoded by the viral oncogene (v-fms) of mcdonough feline sarcoma virus. | the mcdonough strain of feline sarcoma virus (sm-fesv) contains a viral oncogene, v-fms, transduced from cat cellular genetic sequences designated c-fms. monoclonal antibodies reactive to antigenic determinants encoded by v-fms were prepared by immunizing rats with live, syngeneic sm-fesv-transformed cells, and fusing splenic lymphocytes from a tumor-bearing animal with cultured rat myeloma cells. culture supernatants from hybrids producing antibodies to epitopes encoded by v-fms were identified ... | 1982 | 6185513 |
| no expression of a rous sarcoma virus-induced tumor antigen in mammalian cells infected with retroviruses transducing other oncogenes of the src gene family. | immunization with mouse and rat cells transformed by rous sarcoma virus (rsv) or by b77 avian sarcoma virus (asv) induced complete transplantation resistance against an rsv-induced mouse tumor (csa1m) in syngeneic hosts. in contrast, most of the mice immunized with a fujinami sarcoma virus-transformed rat fibroblast line (fsv-3y1), a feline sarcoma virus-transformed cat fibroblast line (fesv-fef), an abelson leukemia virus-infected balb/3t3 cell line (ablv-3t3), or an uninfected 3y1 cell line co ... | 1985 | 2985859 |
| killer cells of feline leukemia virus- and feline sarcoma virus-infected transformed cells: the role of nk, adcc, and in vitro generated cytotoxic cells. | feline white blood cells (wbc) manifested a primary in vitro mixed lymphocyte tumor cell culture (mltc) proliferative response to feline leukemia virus-feline sarcoma virus (felv-fesv)-infected transformed target cells, which reached a peak at day 15. furthermore, primary in vitro mltc cultures generated cytotoxic killer cells capable of killing a variety of targets in non-major histocompatibility gene complex restricted fashion, and effector cells were capable of killing targets introduced repe ... | 1985 | 2992811 |
| molecular cloning and characterization of feline cellular genetic sequences homologous to the oncogene of the mcdonough strain of feline sarcoma virus. | the organization within the cat genome of cellular genetic sequences homologous to the viral oncogene v-fms of the mcdonough strain of feline sarcoma virus (sm-fesv) was determined. four cosmid clones containing overlapping v-fms homologous cellular dna inserts representing a contiguous region of cellular dna of approximately 80 kbp in length have been isolated from a feline cosmid gene library. within this region of the cat genome, the c-fms genetic sequences are dispersed over a region of arou ... | 1985 | 3002022 |
| biological and biochemical characterization of a new isolate of feline sarcoma virus: theilen-pedersen (tp1-fesv). | a new feline sarcoma virus designated theilen-pedersen (tp1-fesv) has been isolated from a spontaneous, slowly growing fibrosarcoma of a domestic short-haired 4-year-old castrated cat. the virus codes for a gag-onc fusion protein of 83,000 molecular weight phosphorylated in vivo at serine, threonine, and tyrosine residues. cells transformed in vitro with tp1-fesv exhibit five- to 10-fold elevated levels of phosphotyrosine over felv-infected cells. the gag-onc polyprotein has associated with it a ... | 1984 | 6208683 |
| feline leukemia virus-and feline sarcoma virus-related polypeptides released by virus producer and nonproducer cells. | polypeptides specific for feline leukemia virus (felv) have been identified in the media of cells that produce felv as well as in nonproducer cells transformed by feline sarcoma viruses (fesv). cat fibroblasts that were persistently infected with felv release the major virus envelope glycoprotein, whereas cultured cat lymphoma cells shed both glycopeptides related to the virus core gene (gag) and glycopeptides related to the virus envelope gene (env). mink cells and cat cells transformed by fesv ... | 1985 | 3002564 |
| a new tumor model for angiographic investigations: feline sarcoma virus tumor induction in adult cats. | this article describes the first successful virus induction of sarcomas in adult immunosuppressed cats. the method of tumor induction, the immunosuppression regimen, and the gross, histologic, and angiographic findings in these induced sarcomas are described. the simplicity of the model, the rapid and reliable growth of the tumors, and the size of the animals permit repeated angiography. the model is well suited to studies of angiographically directed therapy with embolic agents, radioactive imp ... | 1980 | 6246023 |
| molecular cloning of snyder-theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with moloney murine sarcoma virus. | extrachromosomal dna obtained from mink cells acutely infected with the snyder-theilen (st) strain of feline sarcoma virus (feline leukemia virus) [fesv(felv)] was fractionated electrophoretically, and samples enriched for felv and fesv linear intermediates were digested with ecori and cloned in lambda phage. hybrid phages were isolated containing either fesv or felv dna "inserts" and were characterized by restriction enzyme analysis, r-looping with purified 26 to 32s viral rna, and heteroduplex ... | 1980 | 6246254 |
| specific binding of the mononuclear phagocyte colony-stimulating factor csf-1 to the product of the v-fms oncogene. | cells transformed by the mcdonough strain of feline sarcoma virus (sm-fesv) express a v-fms-encoded glycoprotein whose expression at the cell surface correlates with the transformed phenotype. the mouse mononuclear phagocyte growth factor csf-1 specifically binds to sm-fesv-transformed cells at high-affinity sites indistinguishable from those detected on normal feline macrophages. a monoclonal antibody to a v-fms-encoded epitope competed for csf-1 binding to sm-fesv-transformed cells, and chemic ... | 1986 | 3010289 |
| feline sarcoma virus induced in vitro progression from premalignant to neoplastic transformation of human diploid cells. | human diploid cells morphologically transformed by feline sarcoma virus were serially propagated under selective cell culture conditions. when injected into nude mice prior to passage in soft agar (0.35%), morphologically transformed cells did not produce tumors. however, when propagated under selective cell culture conditions, transformed cells grew in soft agar and, when injected subcutaneously into the subcapsular region of the n mu/n mu mice, produced neoplastic nodules histopathologically i ... | 1980 | 6250976 |
| transformation-defective mutants of feline sarcoma virus which express a product of the viral src gene. | mink cell cultures infected with the snyder-theilen strain of feline sarcoma-leukemia virus were cloned from single cells under conditions favoring single virus-single cell interactions. the primary colonies included (i) typical feline sarcoma virus (fesv)-transformed nonproducer clones, one of which segregated revertants, and (ii) fesv-infected, phenotypically normal clones, three of which spontaneously converted to the transformed phenotype. the revertants and spontaneous transformants were co ... | 1980 | 6251261 |
| characterization of a 170,000-dalton polyprotein encoded by the mcdonough strain of feline sarcoma virus. | in this study, we demonstrated the expression of a 170,000-mr polyprotein in each of several mcdonough feline sarcoma virus (fesv)-transformed mink cell clones and one mcdonough fesv-transformed rat clone. this polyprotein designated mcdonough fesv p170, contained feline leukemia virus (felv) p15, p12, and p30 immunological determinants and shared two of its five [35s]methionine-labeled tryptic peptides with felv pr180gag-pol. both of these peptides were shown to be specific to the p30 component ... | 1980 | 6251265 |
| three independent isolates of feline sarcoma virus code for three distinct gag-x polyproteins. | cells nonproductively transformed by the snyder-theilen, gardner-arnstein, and mcdonough strains of feline sarcoma virus synthesize gag-x polyproteins of 78,000, 100,000, and 180,000 daltons, respectively. these feline sarcoma virus-coded products were precipitated by antisera to polypeptides encoded by the gag gene of feline leukemia virus and by rat antisera raised to feline sarcoma virus-transformed rat tumor cells. precipitation with rat antisera absorbed with feline leukemia virus showed th ... | 1980 | 6251274 |
| continuing studies of feline sarcoma virus induced tumors in newborn lambs and in kittens: a model to study comparative neoplasia. | | 1980 | 6253302 |
| a new acute transforming feline retrovirus with fms homology specifies a c-terminally truncated version of the c-fms protein that is different from sm-feline sarcoma virus v-fms protein. | the hz5-feline sarcoma virus (fesv) is a new acute transforming feline retrovirus which was isolated from a multicentric fibrosarcoma of a domestic cat. the hz5-fesv transforms fibroblasts in vitro and is replication defective. a biologically active integrated hz5-fesv provirus was molecularly cloned from cellular dna of hz5-fesv-infected fre-3a rat cells. the hz5-fesv has oncogene homology with the fms sequences of the sm-fesv. the genome organization of the 8.6-kilobase hz5-fesv provirus is 5' ... | 1986 | 3018286 |
| origin and functional properties of the major gene product of the snyder-theilen strain of feline sarcoma virus. | the only known product of the snyder-theilen strain of feline sarcoma virus (st-fesv) is a 85,000-dalton protein, designated st p85, that contains feline leukemia virus gag gene encoded proteins (p15, p12, and a fragment of p30) and a sarcoma virus-specific polypeptide. antibodies directed against the latter immunoprecipitated a 92,000-dalton phosphoprotein (ncp 92) expressed at low levels in normal feline embryo fibroblasts as well as in feline cells of epithelial or lymphoid origin. normal cel ... | 1980 | 6254060 |
| characterization of the human c-fms gene product and its expression in cells of the monocyte-macrophage lineage. | the mcdonough strain of feline sarcoma virus contains an oncogene called v-fms whose ultimate protein product (gp140v-fms) resembles a cell surface growth factor receptor. to identify and characterize the protein product of the proto-oncogene c-fms, antisera were prepared to the viral fms sequences and used to detect specific cross-reacting sequences in human choriocarcinoma cells (bewo) known to express c-fms mrna. both tumor-bearing rat sera and a rabbit antiserum prepared to a segment of v-fm ... | 1985 | 3018521 |
| onc sequences (v-fes) of snyder-theilen feline sarcoma virus are derived from noncontiguous regions of a cat cellular gene (c-fes). | type c sarcoma viruses are genetic recombinants containing portions of replication-competent helper viruses linked to sarcoma virus-specific sequences (generically designated onc genes) which are thought to be required for acute fibroblast transformation. the onc elements of different avian and mammalian sarcoma viral isolates are each homologous to subsets of cellular dna sequences which have no well-defined role in normal cells. because of the lack of significant homology between helper viral ... | 1981 | 6259536 |
| feline uveal melanoma model induced with feline sarcoma virus. | this paper described the first animal model of a virally induced uveal melanoma. tumors developed following the injection of an rna tumor virus, i.e., gardner strain feline sarcoma virus, into the anterior chamber of newborn kittens. histologically, the tumors were found to be iris and ciliary body melanomas, many of which showed invasion. the histology and ultrastructure of those tumors are described. | 1981 | 6260703 |
| cellular transformation by subgenomic feline sarcoma virus dna. | the genome of the snyder-theilen strain of feline sarcoma virus (st-fesv) is a 4.3-kilobase-pair (kbp) rna molecule that contains a 1.5-kbp cellular insertion (fes gene) flanked by feline leukemia virus sequences at its 5' end (1.6 kbp) and 3' end (1.2 kbp) (sherr et al., j. virol. 34:200-212, 1980). dna transfection techniques have been utilized to determine the regions of the st-fesv genome involved in malignant transformation. i have found that the 3.7-kbp 5'-end fragment of the st-fesv provi ... | 1981 | 6261000 |
| structure, expression, and chromosomal location of the human c-fgr gene. | the nucleotide sequence of seven exons of the human c-fgr gene, a cellular homolog of the oncogene of gardner-rasheed feline sarcoma virus, was determined. twenty-six independent genomic clones were obtained from a human gene library with a dna clone of y73 avian sarcoma virus oncogene, v-yes, as a probe under relaxed hybridization conditions. restriction mapping and partial sequence analyses revealed that two of these clones were derived from the c-fgr gene, distinct from the c-yes gene. intere ... | 1986 | 3023853 |
| nucleic acid sequence and oncogenic properties of the hz2 feline sarcoma virus v-abl insert. | hardy-zuckerman 2 feline sarcoma virus (hz2-fesv), isolated from a multicentric feline fibrosarcoma is a replication-defective acute transforming feline retrovirus which originated by transduction of feline c-abl sequences with feline leukemia virus (felv) and is known to encode a 110-kilodalton gag-abl fusion protein with tyrosine-specific protein kinase activity (p. besmer, w. d. hardy, e. e. zuckerman, p. j. bergold, l. lederman, and h. w. snyder, nature (london) 303:825-828, 1983). the nucle ... | 1987 | 3029415 |
| appropriate glycosylation of the fms gene product is a prerequisite for its transforming potency. | processing inhibitors of n-linked glycans were used to determine whether correct glycosylation of the oncogene product gp140v-fms, encoded by the mcdonough strain of feline sarcoma virus (sm-fesv), is required to maintain the oncogenic properties of v-fms. sm-fesv-transformed cells treated with the glucosidase-i inhibitors n-methyldeoxynojirimycin (mdn) or castanospermine synthesized predominantly a gp125v-fms species which had a normal half life. the molecule was transported to the plasma membr ... | 1986 | 3086087 |
| three distinct genes in human dna related to the transforming genes of mammalian sarcoma retroviruses. | southern blot hybridization was used to identify human and other vertebrate dna sequences that were homologous to cloned dna fragments containing the oncogenic nucleic acid sequences of three different type c mammalian retroviruses (simian sarcoma virus, the snyder-theilen strain of feline sarcoma virus, and the harvey strain of murine sarcoma virus). each onc gene counterpart has a single genetic locus, which probably contains non-onc intervening sequences. the human dna sequences may represent ... | 1981 | 6264598 |
| evaluation of the parameters of the elisa procedure for feline type c retroviral antigens: assay of felv antigens in chronically infected or nonproducer transformed cells. | the successful application of an improved enzyme-linked immunosorbent assay (elisa) to the specific detection and quantitation of feline leukemia virus (felv) is presented. the methods described can detect felv antigens at the picogram/milliliter level using a fluorometric assay for peroxidase-linked antibody. the use of a fluorogenic substrate resulted in at least a 9-fold increase in sensitivity of the peroxidase assay. felv antigens could be specifically detected in chronically infected cells ... | 1981 | 6268565 |
| isolation and oncogenic potential of a novel human src-like gene. | we have isolated cdna molecules representing the complete coding sequence of a new human gene which is a member of the src family of oncogenes. nucleotide sequence analysis revealed that this gene, termed slk, encoded a 537-residue protein which was 86% identical to the chicken proto-oncogene product, p60c-src, over a stretch of 191 amino acids at its carboxy terminus. in contrast, only 6% amino acid homology was observed within the amino-terminal 82 amino acid residues of these two proteins. it ... | 1986 | 3099169 |
| expression and characterization of transforming growth factor alpha precursor protein in transfected mammalian cells. | analysis of a cdna clone derived from retrovirus-transformed rat fibroblasts has recently suggested that the mature 50-amino-acid form of transforming growth factor alpha (tgf alpha) is derived from a 159-amino-acid transmembrane precursor by proteolytic cleavage. to understand the processing of the tgf alpha precursor molecule in more detail, we have expressed this protein in baby hamster kidney (bhk) fibroblasts under control of the metal-ion-inducible metallothionein promoter and characterize ... | 1987 | 3299049 |
| characterization of clones of tumorigenic feline cells transformed by a chemical carcinogen. | tumorigenic clonal lines derived from soft agar colonies induced by dmba-transformed feline embryo cells were isolated and characterized. the morphologically altered clonal cells formed large aggregates, growing in this aggregate form when suspended in liquid growth medium above an agar base and forming colonies in soft agar with high efficiency. when inoculated into athymic nude mice, chemically altered clonal cells produced progressively growing sarcomas. cells established from the tumors morp ... | 1981 | 6273330 |
| human cellular fps/fes cdna rescued via retroviral shuttle vector encodes myeloid cell ncp92 and has transforming potential. | we have generated a cdna copy of human c-fps/fes from a 13 kb genomic dna by means of a retroviral shuttle vector, and have begun characterization of its biological and biochemical properties. the cdna was able to direct the in vitro synthesis of a protein that was indistinguishable from myeloid cell c-fps/fes ncp92 by immunoprecipitation with specific antisera, electrophoretic mobility, tryptic fingerprint analysis, and its associated protein kinase activity. when the coding sequence of the gag ... | 1987 | 3329718 |
| neosynthesis of neolacto- and novel ganglio-series gangliosides in a rat fibroblastic cell line brought about by transfection with the v-fes oncogene-containing gardner-arnstein strain feline sarcoma virus-dna. | transfection of retrovirus dna from gardner-arnstein strain feline sarcoma virus containing v-fes oncogene into a rat fibroblastic cell line 3y1 caused not only cell transformation but also a remarkable change in ganglioside expression. the ganglioside phenotype of the 3y1 cells was characterized by the exclusive expression of gm3, along with a trace amount of "a" pathway-related gangliosides (gm2-gm1a-gd1a), whereas in the transfected transformant, 3y1-ga, sialosylparagloboside containing n-ace ... | 1988 | 3349454 |
| synthesis of biologically active transforming growth factor alpha. | a 50-amino acid residue transforming growth factor, type alpha (tgf alpha), secreted in culture by feline-sarcoma-virus-transformed rat embryo fibroblasts, was synthesized by an improved stepwise solid-phase method with an overall yield of 31%. a deprotection strategy based on the sn2 mechanism using either a low concentration of hf or cf3so3h-cf3co2h in dimethylsulfide was employed to remove most of the benzyl-derived protecting groups. the more acid resistant protecting groups of cys and arg w ... | 1987 | 3474216 |
| effect of bacille calmette-guerin immunotherapy on feline sarcoma virus-induced neoplasms in the cat. | kittens infected experimentally with feline sarcoma virus (fesv) were inoculated with emulsions of bacille calmette-guerin (bcg) cell wall preparations to determine the effect of bcg immunotherapy on fesv-induced sarcoma-genesis. the bcg preparations or emulsificant control preparations were administered (i) subcutaneously at the same time and site as was the fesv inoculation, (ii) at the same site but 1 week after fesv inoculation, or (iii) with a mixture of viable autochthonous neoplastic cell ... | 1982 | 6280529 |
| mcdonough feline sarcoma virus: characterization of the molecularly cloned provirus and its feline oncogene (v-fms). | the genetic structure of the mcdonough strain of feline sarcoma virus (sm-fesv) was deduced by analysis of molecularly cloned, transforming proviral dna. the 8.2-kilobase pair sm-fesv provirus is longer than those of other feline sarcoma viruses and contains a transforming gene (v-fms) flanked by sequences derived from feline leukemia virus. the order of genes with respect to viral rna is 5'-gag-fms-env-3', in which the entire feline leukemia virus env gene and an almost complete gag sequence ar ... | 1982 | 6281462 |
| isolation of human oncogene sequences (v-fes homolog) from a cosmid library. | to define the human homolog (or homologs) of transforming sequences (v-fes gene) common to gardner (ga) and snyder theilen (st) isolates of feline sarcoma virus (fesv), a representative library of human lung carcinoma dna in a cosmid vector system was constructed. three cosmid clones were isolated containing ga/st fesv v-fes homologous cellular sequences, within 32- to 42-kilobase cellular inserts representing 56 kilobases of contiguous human cellular dna. sequences both homologous to, and colin ... | 1982 | 6281890 |
| the amino-terminal domain of the v-fms oncogene product includes a functional signal peptide that directs synthesis of a transforming glycoprotein in the absence of feline leukemia virus gag sequences. | the nucleotide sequence of a 5' segment of the human genomic c-fms proto-oncogene suggested that recombination between feline leukemia virus and feline c-fms sequences might have occurred in a region encoding the 5' untranslated portion of c-fms mrna. the polyprotein precursor gp180gag-fms encoded by the mcdonough strain of feline sarcoma virus was therefore predicted to contain 34 v-fms-coded amino acids derived from sequences of the c-fms gene that are not ordinarily translated from the proto- ... | 1986 | 3525854 |
| structure of the feline c-fes/fps proto-oncogene: genesis of a retroviral oncogene. | the nucleotide sequence of the feline c-fes/fps proto-oncogene was analyzed. comparison with v-fes and v-fps revealed that all v-fes/fps homologous sequences were dispersed over 11 kilobase pairs in 19 interspersed segments. all segments, numbered exon 1 to exon 19 as in the chicken and human loci, were flanked by consensus splice junctions. the putative promoter region contained a catt sequence and three ccgccc motifs which were also found in the human locus at similar positions. about 200 nucl ... | 1987 | 3553615 |
| susceptibility of cats, sheep, and swine to a rabbit isolate of encephalitozoon cuniculi. | newborn cats, pigs, and sheep (3 to 14 days old) and postweanling cats (2.5 months old) that had been inoculated with gardner feline sarcoma virus and feline leukemia virus at 10 days of age were infected experimentally with a rabbit isolate of the mammalian protozoan parasite encephalitozoon cuniculi. infection occurred in all cats and in some sheep, but was questionable in pigs. brain and kidney were the 2 major target organs in cats. the lesions were compatible with, but less severe than, tho ... | 1985 | 3923875 |
| nucleotide sequence of fujinami sarcoma virus: evolutionary relationship of its transforming gene with transforming genes of other sarcoma viruses. | we determined the entire nucleotide sequence of the molecularly cloned dna of fujinami sarcoma virus (fsv). the sequence of 1182 amino acids was deduced for the fsv transforming protein p130, the product of the fsv gag-fps fused gene. the p130 sequence was highly homologous to the amino acid sequence obtained for the gag-fes protein of feline sarcoma virus, supporting the view that fps and fes were derived from a cognate cellular gene in avian and mammalian species. in addition, fsv p130 and p60 ... | 1982 | 6291784 |
| the structure of the human c-fes/fps proto-oncogene. | we have determined the complete nucleotide sequence of a human dna fragment of approximately 13 kbp, which was shown by southern blot analysis to contain the entire v-fes/fps cellular homolog. the v-fes/fps homologous sequences were dispersed over 11 kbp in 18 interspersed segments which were flanked by splice junctions. fusion of these segments created a dna fragment in which coding regions similar to those observed in the viral oncogenes v-fes of the gardner-arnstein (ga) and snyder-theilen (s ... | 1985 | 4065096 |
| structural characteristics and nucleotide sequence analysis of genomic rna from rd-114 virus and feline rna tumor viruses. | the results of molecular hybridization experiments have demonstrated that the rna genome of rd-114 virus has extensive nucleotide sequence homology with the rna genome of crandell virus, an endogenous type c virus of cats, but only limited homology with the rna genomes of feline sarcoma virus and feline leukemia virus. the genomic rnas of rd-114 virus and crandell virus also had identical sedimentation coefficients of 50s. a structural rearrangement of genomic rna did not exist within released r ... | 1973 | 4358162 |
| similar transforming growth factors (tgfs) produced by cells transformed by different isolates of feline sarcoma virus. | fisher rat embryo cells transformed by each of three independent isolates of feline sarcoma virus (fesv) are shown to release transforming growth factors (tgfs) into cell culture medium. these acid- and heat-stable peptides compete for binding to, and stimulate phosphorylation of, egf membrane receptors and promote anchorage-independent cell growth. cells transformed by the gardner and synder-theilen strains of fesv produce high titers of tgf (60-200 ng eq egf/liter) while cells transformed by m ... | 1983 | 6297155 |
| type beta transforming growth factor from feline sarcoma virus-transformed rat cells. isolation and biological properties. | we have isolated a strongly mitogenic, type beta transforming growth factor (beta tgf) released by snyder-theilen feline sarcoma virus-transformed rat embryo (fesv-fre) cells that induces phenotypic transformation of normal nrk cells when they are concomitantly stimulated by analogues of epidermal growth factor (egf). molecule filtration chromatography separates beta tgf from an egf-like tgf (etgf) which is also present in acid extracts from medium conditioned by fesv-fre cells (j. massagué, (19 ... | 1984 | 6086646 |
| transforming genes of avian (v-fps) and mammalian (v-fes) retroviruses correspond to a common cellular locus. | the gardner (ga) and snyder-theilen (st) isolates of feline sarcoma virus (fesv) represent genetic recombinants between feline leukemia virus (felv) and transformation-specific sequences (v-fes gene) of cat cellular origin. a related transforming gene (v-fps), common to the fujinami, prc ii, and ur 1 strains of avian sarcoma virus has also been described. translational products of each of these recombinant virus isolates are expressed in the form of polyproteins exhibiting protein kinase activit ... | 1983 | 6301150 |
| the parodi-irgens feline sarcoma virus and simian sarcoma virus have homologous oncogenes, but in different contexts of the viral genomes. | we have identified the oncogene and the putative transforming protein of the parodi-irgens feline sarcoma virus (pi-fesv). the pi-fesv is defective and needs a helper virus for its replication. the v-onc sequences in the pi-fesv were found to be related to the v-sis sequences of the simian sarcoma virus (ssv). pi-fesv nonproducer cells express two viral rnas, a 6.8-and a 3.3-kilobase rna. the 6.8-kilobase rna contains gag, sis, and env sequences but lacks the pol gene. the 3.3-kilobase rna, on t ... | 1983 | 6302323 |
| regulation of viral and cellular oncogene expression by cytosine methylation. | mink cells morphologically transformed by either snyder-theilen feline sarcoma virus (st-fesv) or abelson murine leukemia virus (abelson-mulv) exhibit relatively high rates of reversion to the nontransformed phenotype. the proviral dnas are conserved within the revertant lines and have not undergone changes in integration sites due to translocations or other genomic rearrangements. in contrast, expression of well-defined viral-encoded transforming proteins is blocked and elevated levels of phosp ... | 1983 | 6302983 |
| in vitro transformation of murine pre-b lymphoid cells by snyder-theilen feline sarcoma virus. | snyder-theilen feline sarcoma virus (st-fesv) codes for a protein kinase with specificity for tyrosine residues (barbacid et al., proc. natl. acad. sci. u.s.a. 77:5158-5163, 1980), properties analogous to those of the transforming gene product of abelson murine leukemia virus (witte et al., nature (london) 283:826-831, 1980). in the present report, st-fesv was demonstrated to transform murine hematopoietic cells under in vitro assay conditions which detect lymphoid cell transformation by abelson ... | 1983 | 6304354 |
| active immunization with feline leukemia virus envelope glycoprotein suppresses growth of virus-induced feline sarcoma. | the efficiency was examined of immunization with feline leukemia virus glycoprotein complexes (gp85 rosettes) to protect cats against tumors induced by feline sarcoma virus (fesv). the glycoprotein was isolated from feline leukemia virus (felv). young cats were vaccinated with the purified viral glycoprotein and challenged with fesv (felv). felv gp85 antibody levels were measured by enzyme-linked immunosorbent assay and tumor volumes were determined. in immunized animals tumor development was re ... | 1983 | 6304480 |
| biochemical and immunological characterization of polyproteins coded for by the mcdonough, gardner-arnstein, and snyder-theilen strains of feline sarcoma virus. | the mcdonough (sm), gardner-arnstein (ga), and snyder-theilen (st) strains of feline sarcoma virus (fesv) code for high-molecular-weight polyproteins that contain varying amounts of the amino-terminal region of the felv gag gene-coded precursor protein and a polypeptide(s) of an as yet undetermined nature. the sm-fesv primary translational product is a 180,000-dalton polyprotein which is immediately processed into a highly unstable 60,000-dalton molecule containing the p15-p12-p30 fragment of th ... | 1980 | 6154148 |
| nucleotide sequences of feline retroviral oncogenes (v-fes) provide evidence for a family of tyrosine-specific protein kinase genes. | the nucleotide sequences encoding the transforming polyproteins of the snyder-theilen and gardner-arnstein strains of feline sarcoma virus (fesv) have been determined. these sequences include a viral transforming gene (v-fes), derived from cellular proto-oncogene sequences (c-fes) of domestic cats by recombination with feline leukemia virus (felv). the v-fes sequences are predicted to encode a polypeptide domain strikingly similar to that specified by the transforming gene (v-fps) of the avian f ... | 1982 | 6183005 |
| transcription of c-onc genes c-raski and c-fms during mouse development. | we investigated the expression of cellular sequences c-raski and c-fms, which are homologous to the oncogenes of kirsten rat sarcoma virus and the mcdonough strain of feline sarcoma virus, during murine development and in a variety of mouse tissues. the c-raski gene was found to be transcribed into two mrna species of approximately 2.0 and 4.4 kilobases, whereas a single c-fms-related transcript of approximately 3.7 kilobases was identified. the c-raski gene appeared to be expressed ubiquitously ... | 1983 | 6308423 |
| monoclonal antibodies specific to transforming polyproteins encoded by independent isolates of feline sarcoma virus. | hybridomas secreting monoclonal antibodies directed against polyprotein gene products of the gardner, snyder-theilen, and mcdonough strain of feline sarcoma virus have been isolated. antibody produced by one hybridoma recognizes immunological determinants localized within a feline leukemia virus gag gene structural component (p15) common to polyproteins encoded by each feline sarcoma virus isolate while antibody produced by a second is specific for p30 determinants unique to p170gag-fms. additio ... | 1982 | 6183442 |
| molecular cloning of integrated gardner-rasheed feline sarcoma virus: genetic structure of its cell-derived sequence differs from that of other tyrosine kinase-coding onc genes. | gardner-rasheed feline sarcoma virus (gr-fesv) is an acute transforming retrovirus which encodes a gag-onc polyprotein possessing an associated tyrosine kinase activity. the integrated form of this virus, isolated in the charon 21a strain of bacteriophage lambda, demonstrated an ability to transform nih/3t3 cells at high efficiency upon transfection. foci induced by gr-fesv dna contained rescuable sarcoma virus and expressed gr-p70, the major gr-fesv translational product. the localization of lo ... | 1983 | 6312085 |
| feline sarcoma virus-specific transformation-related proteins and protein kinase activity in tumor cells. | polyproteins (gag-fes) encoded by the synder-theilen (st) and the gardner-arnstein (ga) strains of feline sarcoma virus (fesv) were previously shown to be associated with mink or rat cells that were nonproductively transformed in vitro. in the present study we demonstrated that the same gag-fes proteins were found in cat cells transformed in vitro. of greater importance, these transformation-related proteins were also in cells taken from fresh biopsies of fesv-induced tumors. cells from fibrosar ... | 1983 | 6186075 |
| the feline oncornavirus-associated cell membrane antigen (focma) is related to, but distinguishable from, felv-c gp70. | the feline oncornavirus-associated cell membrane antigen (focma) on the surface of feline lymphosarcoma (lsa) cells is defined as the target(s) recognized in immunofluorescence (ifa) tests by antibody in sera of cats relatively resistant to development of felv (feline leukemia virus) lsa and fesv (feline sarcoma virus) fibrosarcoma. the specificities of antibodies in cat focma-typing sera and the nature of the lsa antigens recognized were investigated in the present study. focma sera obtained fr ... | 1983 | 6318434 |
| feline oncornavirus-associated cell membrane antigen: a viral and not a cellularly coded transformation-specific antigen of cat lymphomas. | the feline oncornavirus-associated cell membrane antigen (focma) was defined as a tumor antigen common to cat lymphomas and fibrosarcomas induced by feline leukemia virus (felv) and feline sarcoma virus (fesv), respectively. the antigen was recognized by sera from cats thought to be resistant to leukemogenesis. we report here that a common denominator in the activity of naturally occurring viremic cat antisera to focma is, in fact, their reactivity to felv c antigenic determinants. the cat antis ... | 1983 | 6186079 |
| isolation of a new feline sarcoma virus (hz1-fesv): biochemical and immunological characterization of its translation product. | a new strain of feline sarcoma virus, designated hz1-fesv, was isolated from a 4-year-old domestic cat with multicentric fibrosarcoma. a primary tumor cell line was established and virus produced from that line was found to induce foci in feline embryonic lung fibroblasts (flf3) and mink lung fibroblasts (ccl64) in tissue culture and fibrosarcomas in inoculated 10-week-old kittens. the derivation of transformed nonproducer clones of flf3 and ccl64 cells containing helper virus-rescuable, focus-f ... | 1984 | 6320533 |
| association of the transforming proteins of the st and ga strains of feline sarcoma virus and their in vitro associated protein kinase activities with cellular membranes. | the translation products of the snyder-theilen (st) and gardner-arnstein (ga) strains of feline sarcoma virus (fesv), termed gag-fes proteins, are high molecular weight polyproteins containing different amounts of the amino terminus of the feline leukemia virus (felv) gag gene-coded precursor protein linked to a similar sarcoma virus-specific polypeptide. both polyproteins are phosphoproteins with indistinguishable in vitro associated tyrosine-specific protein kinase activities. the polyproteins ... | 1983 | 6320992 |
| detection and localization of a phosphotyrosine-containing onc gene product in feline tumor cells. | protein phosphorylation by a tyrosine-specific kinase is now recognized as a common event in retrovirus-transformed cells. we report in this communication that the feline sarcoma virus (fesv) encoded transformation-specific proteins (gag-fes fusion proteins) and their associated protein kinases are also found in the fesv in vivo induced tumor preparations, either in the form of fresh tumor homogenate or in the form of cultured cells. with the combined use of subcellular fractionation and deterge ... | 1983 | 6190715 |
| monoclonal antibodies to the transforming protein of fujinami avian sarcoma virus discriminate between different fps-encoded proteins. | two monoclonal antibodies have been obtained that recognize antigenic determinants within the c-terminal fps-encoded region of p140gag-fps, the transforming protein of fujinami avian sarcoma virus (fsv). the hybridomas which secrete these antibodies (termed 88ag and p26c) were isolated after the fusion of ns-1 mouse myeloma cells with b lymphocytes from fischer rats that had been immunized with fsv-transformed rat-1 cells. fsv p140gag-fps immunoprecipitated by either antibody is active as a tyro ... | 1984 | 6323756 |
| translational products encoded by newly acquired sequences of independently derived feline sarcoma virus isolates are structurally related. | polyproteins encoded by several independent isolates of feline sarcoma virus (fesv) were analyzed with respect to molecular weight, extent of phosphorylation, and tryptic peptide composition. as previously reported, cells nonproductively transformed by the gardner strain of fesv express a polyprotein which has a molecular weight of approximately 115,000 and contains feline leukemia virus p15, p12, and minor portion of p30. in addition, a major 72,000-dalton possible cleavage product can be ident ... | 1980 | 6245259 |
| feline sarcoma virus polyprotein p115 binds a host phosphoprotein in transformed cells. | several independent isoltes of feline sarcoma virus (fesv) have been described. such viruses are apparently derived by genetic recombination between feline leukaemia virus (felv) genomic rna and host cellular genetic sequences with transforming potential. two fesv isolates, one originally described by gardner and the second by snyder-theilen, have been shown to encode polyproteins of around 115,000 molecular weight. both polyproteins contain felv structural components (p15, p12) at their amino t ... | 1980 | 6250064 |
| infection with feline leukemia virus associated with induction of humoral response to a normal cell protein. | selected populations of cats that were naturally exposed to the feline leukemia virus (felv) were found to have humoral antibodies to a normal cell protein designated ncp105. earlier studies revealed that cats exposed to felv often had serum antibodies to the feline oncornavirus-associated cell membrane (focma) as well as to a feline sarcoma virus (fesv) -specific transforming protein designated gag-fes. cats with no history of exposure to felv or fesv lacked antibodies to all three antigens: nc ... | 1984 | 6329486 |
| protection of cats against progressive fibrosarcomas and persistent leukemia virus infection by vaccination with feline leukemia cells. | young cats (3-6 mo old) were challenged with oncogenic snyder-theilen feline sarcoma virus (fesv) after vaccination with live or killed fl74 cat lymphoma cells. compared with controls immunized with normal cat fibroblasts, the fl74-vaccinated cats exhibited increased resistance to fesv-induced progressive primary and disseminated secondary tumors. maximum protection was achieved by vaccination with live fl74 cells or with a low dose of freeze-thawed cells, but tumor cells inactivated by glutaral ... | 1980 | 6253714 |
| homology exists among the transforming sequences of avian and feline sarcoma viruses. | fujinami sarcoma virus (fsv) of chickens does not contain nucleotide sequences related to the src gene of rous sarcoma virus, but it carries unique sequences of at least 3000 bases, which are likely to code for the transforming protein of this virus. using radioactive dna complementary to fsv-unique sequences, we investigated the relatedness of fsv to other sarcoma-leukemia retroviruses in vertebrates. under conditions of moderate stringency, no cross-hybridization was detected between fsv cdna ... | 1980 | 6256742 |
| involvement of a high-molecular-weight polyprotein translational product of snyder-theilen feline sarcoma virus in malignant transformation. | the previously described high-molecular-weight polyprotein major translational product of the snyder-theilen strain of feline sarcoma virus (fesv) was shown to possess protein kinase activity with specificity for tyrosine acceptor sites. cells transformed by snyder-theilen fesv exhibited constitutively elevated levels of phosphotyrosine and a concomitant reduction in epidermal growth factor (egf) binding sites. by endpoint cloning in microtiter plates, a number of transformation-defective (tf) m ... | 1981 | 6261011 |
| transforming proteins of some feline and avian sarcoma viruses are related structurally and functionally. | transformation of chicken cells by fujinami sarcoma virus (fsv), prc ii or y73 (three independently isolated avian sarcoma viruses that are replication-defective and lack the rous sarcoma virus src gene) resulted in significant elevation (4-13 fold) of phosphotyrosine levels in cellular protein. the gag-related proteins encoded by these avian sarcoma viruses (asvs) were all associated with tyrosine-specific protein kinase activity when assayed in immune complexes and were phosphorylated at both ... | 1981 | 6263483 |
| biochemical characterization of cells transformed via transfection by feline sarcoma virus proviral dna. | murine fibroblasts transformed by transfection with dna from mink cells infected with the snyder-theilen strain of feline sarcoma virus and subgroup b feline leukemia virus were analyzed for the presence of integrated proviral dna and the expression of feline leukemia virus- and feline sarcoma virus-specific proteins. the transformed murine cells harbored at least one intact feline sarcoma virus provirus, but did not contain feline leukemia virus provirus. the transformed murine cells expressed ... | 1981 | 6264144 |
| chromosomal localization of the human c-fms oncogene. | a molecular probe was prepared with specificity for the human cellular homologue of transforming sequences represented within the mcdonough strain of feline sarcoma virus (v-fms). by analysis of a series of mouse-human somatic cell hybrids containing variable complements of human chromosomes it was possible to assign this human oncogene, designated c-fms, to chromosome 5. regional localization of c-fms to band q34 on chromosome 5 was accomplished by analysis of chinese hamster-human cell hybrids ... | 1983 | 6684766 |
| feline uveal melanomas induced with feline sarcoma virus: potential model of the human counterpart. | uveal melanomas were produced by injecting gardner strain feline fibrosarcoma virus intraocularly into 10- to 15-day-old noninbred kittens. tumors developed in about 90% of the cats' eyes receiving virus. progressing tumors (62 eyes of 36 cats) began as small hyperpigmented lesions at the site of injection and grew to fill the anterior chamber by 3-5 months after infection. about 30% of cats with these tumors developed secondary tumors and died. nonprogressive tumors characterized by flat, pigme ... | 1981 | 6268880 |
| genetic mapping of the mouse oncogenes c-ha-ras-1 and c-fes to chromosome 7. | the mouse homologs of the cellular oncogenes c-ha-ras-1 of harvey sarcoma virus and c-fes of feline sarcoma virus were both mapped to chromosome 7 by southern blot analysis of hamster-mouse somatic cell hybrid dnas. | 1983 | 6864883 |
| recombinant bacteriophages containing the integrated transforming provirus of gardner--arnstein feline sarcoma virus. | the integrated dna provirus of the gardner-arnstein (ga) strain of feline sarcoma virus (fesv) was molecularly cloned in a bacteriophage lambda vector. the cloned dna fragment is 14.4 kilobase pairs long and contains a 6.7-kilobase provirus flanked by cellular sequences derived from nonproductively transformed mink cells. transfection of mouse nih/3t3 cells with the cloned dna fragment induced foci of transformation at efficiencies of 10(4) focus-forming units/pmol of sarcoma virus dna. restrict ... | 1981 | 6270655 |
| relationship of the feline oncornavirus associated cell membrane antigen to a feline sarcoma virus encoded polyprotein. | | 1981 | 6274763 |
| human gene (c-fes) related to the onc sequences of snyder-theilen feline sarcoma virus. | the onc gene (v-fes) of the acutely transforming feline sarcoma virus (snyder-theilen strain) has homologous cellular sequences (c-fes) in all vertebrate species, including humans. we isolated from a human dna library recombinant phages containing overlapping c-fes sequences. the human c-fes locus spans a region of 3.4 kilobases and contains 1.4 kilobases of dna homologous to the viral onc sequence interspersed with three intervening sequences. | 1982 | 6290870 |
| feline sarcoma virus p115-associated protein kinase phosphorylates tyrosine. identification of a cellular substrate conserved during evolution. | the gardner strain of feline sarcoma virus (fesv) has previously been shown to encode as its major translational product, a phosphoprotein of molecular weight (mr) = 115,000 (p115) with an associated protein kinase activity and probable transforming function. in the present study, we demonstrate that this enzyme specifically phosphorylates tyrosine and that gardner fesv-transformed mink and rat cells exhibit constitutively higher levels of phosphotyrosine than nontransformed control cells. in ad ... | 1980 | 7430166 |
| monoclonal antibodies to the transformation-specific glycoprotein encoded by the feline retroviral oncogene v-fms. | monoclonal antibodies prepared to epitopes encoded by the transforming gene (v-fms) of the mcdonough strain of feline sarcoma virus were used to study v-fms-coded antigens in feline sarcoma virus-transformed rat and mink cells. these antibodies reacted with three different polypeptides (gp180gag-fms, gp140fms, and gp120fms), all of which were shown to be glycosylated. protein blotting with [125i]-labeled monoclonal immunoglobulin g's was used to determine the relative steady-state levels of thes ... | 1982 | 6292527 |
| nucleotide sequences of feline sarcoma virus long terminal repeats and 5' leaders show extensive homology to those of other mammalian retroviruses. | the nucleotide sequences of the gardner-arnstein feline sarcoma virus (fesv) long terminal repeat and the adjacent leader sequences 5' to the viral gag gene were determined. these were compared with homologous portions of synder-theilen fesv and with previously published sequences for moloney murine sarcoma virus and simian sarcoma virus proviral dna. more than 75% of the residues in the fesv r and u5 regions were homologous to sequences within the same regions of the other viral long terminal r ... | 1983 | 6296453 |
| mutant feline sarcoma proviruses containing the viral oncogene (v-fes) and either feline or murine control elements. | the sequences required for transformation by the gardner-arnstein (ga) strain of feline sarcoma virus (ga-fesv) were defined by site-directed, in vitro mutagenesis of molecularly cloned proviral dna. portions of the ga-fesv provirus, subcloned in the plasmid pbr322, were mutagenized by deletion or frameshift at xhoi restriction sites flanking the nucleotide sequences presumed to encode the ga-fesv transforming polyprotein (p108(gag-fes)). the biological activity of subgenomic and reconstructed f ... | 1983 | 6300443 |
| conversion of human fibroblasts to tissue macrophages by the snyder-theilen feline sarcoma virus (st:fesv(felv)): productive infection by leishmania major. | the decisive role of macrophages in t-cell differentiation is best exemplified by cutaneous leishmaniasis. during infection, leishmania attach to macrophages, the only site of replication for the parasite. we have recently demonstrated the conversion of human fibroblasts to tissue macrophages (tm) by transduction with the snyder-theilen feline sarcoma virus (st:fesv-(felv)). since leishmania have tropism only for macrophages, we have used the parasite to ascertain the functional phenotype of the ... | 1994 | 7721332 |
| the hardy-zuckerman 2-fesv, a new feline retrovirus with oncogene homology to abelson-mulv. | there is substantial evidence that oncogenes (v-onc) of acute transforming retroviruses have been acquired by transduction of cellular genes (c-onc) with retroviruses. feline leukaemia virus (felv)-associated feline fibrosarcomas have proven to be extremely useful for the isolation of acute transforming retroviruses of a mammalian species. three different v-onc genes have been identified in five acute transforming feline retroviruses. the susan mcdonough feline sarcoma virus (smfesv) contains th ... | 1983 | 6306469 |
| suppression of retroviral tumors in hosts immune to viral oncogene product. | feline sarcoma virus of snyder-theilen strain (st-fesv) induces sarcomas in wistar/ma rats following neonatal virus injection. induced tumors express the viral oncogene product (p85) and elicit in hosts the specific serum anti-p85 antibody detectable by western blot analysis. syngeneic adult female rats were immunized with an st-fesv induced sarcoma that was 100% transplantable to syngeneic adult rats. newborns from immunized rats (vaccinated rats) were found to carry anti-p85 in their sera at b ... | 1994 | 8152290 |
| monoclonal antibodies to feline sarcoma virus gag and fes gene translational products. | a series of hybridomas have been isolated which produce monoclonal antibodies directed against polyprotein gene products of the gardner, snyder-theilen, and mcdonough strains of fesv. within these are representatives of several immunoglobulin classes including igg1, igg2a, igg2b, and igm. antibody produced by one hybridoma recognizes immunologic determinants localized within an felv gag gene structural component (p15) common to polyproteins encoded by all three fesv isolates whereas antibody pro ... | 1983 | 6308016 |
| feline cytotoxic immune mechanisms against virus-associated leukemia and fibrosarcoma. | humoral and cellular cytotoxic immune mechanisms of cats were compared against feline leukemia virus (felv)- and feline sarcoma virus (fesv)-transformed cells. the groups of animals studied were nonexposed control cats; felv-infected immune or viremic tumor-bearing cats; fesv-inoculated tumor progressor or regressor cats, and cats immunized with fesv-transformed autochthonous fibroblasts (atf). sera containing complement-dependent antibodies (cda), which lysed felv-producer lymphoma lines, had n ... | 1983 | 6311439 |
| epidermal growth factor receptor metabolism and protein kinase activity in human a431 cells infected with snyder-theilen feline sarcoma virus or harvey or kirsten murine sarcoma virus. | when human a431 cells, which carry high numbers of epidermal growth factor (egf) receptors, are exposed to egf, the total content of phosphotyrosine in cell protein is increased, the egf receptor becomes phosphorylated at tyrosine, and new phosphotyrosine-containing 36,000- and 81,000-dalton proteins are detected. we examined the properties of a431 cells infected with snyder-theilen feline sarcoma virus, whose transforming protein has associated tyrosine protein kinase activity, and harvey and k ... | 1983 | 6313970 |
| use of immunohistochemistry and polymerase chain reaction for detection of oncornaviruses in formalin-fixed, paraffin-embedded fibrosarcomas from cats. | to determine whether there was intralesional infection or expression of felv or feline sarcoma virus in suspected vaccine-associated fibrosarcomas in cats. | 1996 | 8756876 |
| biological behavior of tumors and associated retroviremia in cats inoculated with snyder-theilen fibrosarcoma virus and the phenomenon of tumor recurrence after primary regression. | the fate of tumors and associated retroviremia was studied in 111 cats infected with the snyder-theilen strain of feline sarcoma virus (fesv). tumors appeared at the site of inoculation within 7 to 10 days. a retroviremia, due mainly to the associated feline leukemia virus helper virus (felv-helper), developed at the same time as tumors. of the cats, 44 developed progressively growing tumors and therefore had to be killed, and 67 developed tumors that regressed. there was a strong correlation be ... | 1984 | 6319286 |
| localization of the cellular oncogenes abl, sis, and fes on human germ-line chromosomes. | the human germ-line positions of the oncogenes abl, sis, and fes, the cellular counterparts of the v-onc genes of abelson murine leukemia virus, simian sarcoma virus, and feline sarcoma virus, respectively, have been determined by in situ molecular hybridization of 3h-labeled v-onc gene probes to meiotic pachytene chromosomes. the position of abl at 9q34.1 corresponds to the breakpoint in chromosome 9 in the translocation that gives rise to the philadelphia chromosome, t(9;22) (q34; q11); the po ... | 1984 | 6323103 |
| the use of immunohistochemistry and the polymerase chain reaction for detection of feline leukemia virus and feline sarcoma virus in six cases of feline ocular sarcoma. | ocular sarcoma was diagnosed by light microscopic examination in enucleated globes (n = 4), orbital tissue biopsy (n = 1) and ocular evisceration contents (n = 1) from six cats. to determine if feline leukemia virus (felv) or a replication-defective felv, feline sarcoma virus (fesv), was present in these ocular sarcomas, immunohistochemistry (ihc) and polymerase chain reaction (pcr) for felv were utilized. immunohistochemical staining for felv glycoprotein 70 (gp70) was performed on all six form ... | 1998 | 11397230 |
| interaction of feline sarcoma virus (fesv) and mycoplasma. | a factor present in the supernatant of an established human glioma cell line u-251 mg strongly suppresses feline sarcoma virus (fesv) focus forming activity on feline embryo fibroblasts. the factor was identified as mycoplasma arginini. the enriched mycoplasma fraction had no cytpathogenic effect on the glioma cells or on the embryonic feline indicator cells. an antiserum prepared against this strain of mycoplasma abolished the inhibition. the exact mechanism is not known but arginine depletion ... | 1981 | 6274139 |
| isolation and characterization of a human locus homologous to the transforming gene (v-fes) of feline sarcoma virus. | a single locus (designated c-fes) in the human genome which exhibits homology to the transformation-specific onc gene (v-fes) of snyder-theilen feline sarcoma virus was identified by the southern blot technique. recombinant clones containing 16- to 18-kilobase inserts of human dna including the c-fes locus were constructed. restriction endonuclease mapping of these clones verified their identity with native human c-fes and demonstrated the presence of at least two sequences in human c-fes interr ... | 1982 | 6277911 |
| molecular cloning of the fujinami sarcoma virus genome and its comparison with sequences of other related transforming viruses. | full-length proviral dna of fujinami sarcoma virus (fsv) of chickens was molecularly cloned and characterized. an analysis of fsv dna integrated in mammalian cells showed that restriction endonuclease saci has a single cleavage site on fsv dna. unintegrated closed circular fsv dna obtained from newly infected cells was linearized by digestion with saci and cloned into lambdagtwes.lambdab. the following three different molecules were isolated: fsv-1 (4.4 kilobases [kb]) and fsv-2 (4.7 kb), which ... | 1982 | 6284986 |
| multicentric fibrosarcoma in a cat and a review of the literature. | a case of multicentric fibrosarcoma in a ten month old domestic short-haired cat is presented and discussed. tumor tissue was found to involve the right distal forepaw, right shoulder area and a popliteal lymph node. this anaplastic neoplasm was concentrated primarily in subcutaneous tissues but also extended to muscle, bone and lung. the cat was found to be positive for feline leukemia virus by the elisa test. based on these findings, it is likely that the lesions in this case result from an in ... | 1984 | 17422403 |
| feline vaccine-associated fibrosarcoma induced by aluminium compound in two cats: short communication. | two cases of feline vaccine-associated fibrosarcoma (fvaf) are reported. the excised tumours were both characterised as well circumscribed, subcutaneous, firm and white with central necrosis. histopathologically, they consisted of well-differentiated and variably sized and shaped anaplastic cells, characterised by marked nuclear and cellular pleomorphism including giant cells. the mitotic activity was low. aluminium was demonstrated in the central necrosis and giant cells. neoplastic cells were ... | 2008 | 18401961 |
| chromosomal assignment of the human homologues of feline sarcoma virus and avian myeloblastosis virus onc genes. | retroviral transforming genes, v-onc genes, are derived from normal cellular sequences that are called cellular onc (c-onc) genes. dna from mouse-human somatic cell hybrids that have selectively lost human chromosomes was used in southern blots to map the chromosomal location of two human onc genes. cloned human homologues of retroviral onc genes were used as probes. because the human c-fes gene, which is homologous to feline sarcoma virus, segregates concordantly with human chromosome 15, and t ... | 1982 | 6289315 |
| prediction of kit mutation in gastrointestinal stromal tumors by the immunoprofile of the tumor cells. | human kit protooncogene is the cellular homolog of v-kit from the hardy-zuckerman 4 feline sarcoma virus, and encodes a 145-kda type iii tyrosine kinase growth factor receptor that is often mutated in gastrointestinal stromal tumors (gist). standardized mutation analysis is not available in many countries; therefore, we aimed to determine if the presence of kit mutation in gist can be predicted by the immunoprofile of the tumor cells. | 2010 | 20123583 |