| cross-linked double-stranded rna from a defective vesicular stomatitis virus particle. | | 1976 | 178094 |
| phenotypically mixed vesicular stomatitis virus particles produced in human tumor cell lines. | | 1975 | 169045 |
| phenotypic mixing between vesicular stomatitis virus and avian rna tumor viruses. | | 1975 | 169046 |
| vesicular stomatitis virus: structure and function of virion components. | | 1976 | 178479 |
| conditional lethal mutants of vesicular stomatitis virus. | | 1975 | 169102 |
| host effect on vesicular stomatitis virus morphogenesis and "t" particle formation in reptilian, avian, and mammalian cell lines. | the morphogenesis of vesicular stomatitis virus (vsv) in three reptilian cell lines (turtle heart, gecko lung, and viper spleen) was studied by thin section electron microscopy. simultaneous growth studies were conducted in reptilian, chick embryo fibroblast, and bhk/21 cells to compare the yields of infectious vsv during serial passages. the mean length of gecko lung cell vsv measured from electron micrographs is statistically significantly shorter than that of vsv replicating in other reptilia ... | 1975 | 170198 |
| inhibition of pseudorabies virus replication by vesicular stomicles virus i. activity of infectious and inactivated b particles. | infectious b particles of vesicular stomatitis virus (vsv) are capable of inhibiting the replication of pseudorabies virus (psr) in a variety of cell lines. even under conditions of an abortive infection in a continuous line of rabbit cornea cells (rc-6o), b particles interfere with the replication of psr with high efficiency. particle per cell dose-response analysis of b particle populations revealed that the number of vsv particles capable of inhibiting psr replication exceeds the number of pf ... | 1976 | 178894 |
| inhibition of pseudorabies virus replication by vesicular stomatitis virus. ii activity of defective interfering particles. | purified defective interfering (di) particles of vesicular stomatitis virus (vsv) inhibit the replication of a heterologous virus, pseudorabies virus (psr), in hamster (bhk-21) and rabbit (rc-60) cell lines. in contrast to infectious b particles of vsv, uv irradiation of di particles does not reduce their ability to inhibit psr replication. however, uv irradiation progressively reduces the ability of di particles to cause homologous interference with b particle replication. pretreatment with int ... | 1976 | 178895 |
| effect to tween 80 and aquasol a on virus plaque formation. | the enhancement of vesicular stomatitis virus plaques on human embryonic lung cells in the presence of tween 80 or aquasol a was studied to determine the optimal conditions for the enhancement. enhanced numbers and sizes of vesicular stomatitis virus plaques occurred when aquasol a or tween 80 was added to the cell culture 30 min before virus adsorption but not when added after adsorption. these substances did not have a direct effect on the virus and did not have an effect on virus adsorption o ... | 1975 | 170202 |
| rna- temperature-sensitive mutants of vesicular stomatitis virus: l-protein thermosensitivity accounts for transcriptase restriction of group i mutants. | in vitro transcriptase activity of three group i temperature-sensitive (ts) mutants of vesicular stomatitis virus restricted at 39 c was restored by l-protein fractions derived from wild-type (wt) vesicular stomatitis virion nucleo-capsids. soluble ns protein from wt nucleocapsids did not reconstitute restricted transcriptions of the group i rna-ts mutants. ns protein activity, but not l protein activity, was purified from the group i ts mutants; this ns fraction always displayed the wt phenotyp ... | 1976 | 178900 |
| cyclic amp potentiation of interferon antiviral activity and effect of interferon on cellular cyclic amp levels. | treatment of l cells with 3 to 10 mm 3':5'-cyclic adenosine monophosphate (camp) in the presence of interferon was found to potentiate the development of antiviral activity. the dose response of interferon activity at various time periods in the presence and absence of camp indicated that potentiation of interferon activity by camp occurred at an early stage in the development of antiviral activity. among the analogues of camp tested for interferon-potentiating activity, only the acylated deriva ... | 1975 | 170377 |
| control of vesicular stomatitis virus protein synthesis. | | 1976 | 179201 |
| sulfated components of enveloped viruses. | the glycoproteins of several enveloped viruses, grown in a variety of cell types, are labeled with 35so4(-2), whereas the nonglycosylated proteins are not. this was shown for the hn and f glycoproteins of sv5 and sendai virus, the e1 and e2 glycoproteins of sindbis virus, and for the major glycoprotein, gp69, as well as for a minor glycoprotein, gp52, of rauscher leukemia virus. the minor glycoprotein of rauscher leukemia virus is more highly sulfated, with a ratio of 35so4- [3h]glucosamine abou ... | 1975 | 170420 |
| further studies on the relative antiviral efficacies of interferons induced by poly i:c and mengo virus. | mouse serum interferons induced by polyi:c, vesicular stomatitis virus (vsv), reovirus, and mengo virus were assayed in monolayers of mouse l-929 cells by the plaque-reduction method using both vsv and mengo as challenge viruses. titers obtained with mengo virus as challenge were all lower than with vsv. with the interferons induced by vsv, reovirus, and ployi:c, the reductions were of the order of two- to three-fold. with mengo virus-induced interferon the reduction was much greater (about 17-f ... | 1976 | 179687 |
| comparison of central nervous system disease produced by wild-type and temperature-sensitive mutants of vesicular stomatitis virus. | the pathogenicity of infection produced following intracerebral (i.c.) inoculation of wild-type vesicular stomatitis virus (vsv) or temperature-sensitive (ts) mutants of vsv was compared. ts mutants used were ts 31 (vsv complementation group ii) and ts 41 (vsv complementation group iv). the i.c. injection of wild-type vsv in weanling swiss mice produced a rapidly fatal encephalitis with death of mice in 2 to 3 days. histopathologically, such mice exhibited minimal changes of encephalitis on ligh ... | 1976 | 179953 |
| coupled in vitro transcription and translation of vesicular stomatitis virus messenger rna. | the virion transcriptase (nucleosidetriphosphate: rna nucleotidyltransferase, ec 2.7.7.6) of vesicular stomatitis virus was fully active when ribonucleoprotein cores from purified virions were added to cell-free protein synthesizing systems of eukaryotic origin. synthesis of mrna was linear for at least 3 hr and the newly synthesized viral mrna was efficiently utilized for the synthesis of viral proteins n (nucleoprotein), ns, and m (matrix); small amounts of a putative g (glycoprotein protein p ... | 1975 | 170604 |
| inhibition by aurintricarboxylic acid and polyethylene sulfonate of rna transcription of vesicular stomatitis virus. | the in vitro activity of the ribonucleoprotein-dependent rna transcriptase of vesicular stomatitis virions was found to be completely inhibited by low concentrations of aurintricarboxylic acid (ata) and polyethylene sulfonic acid (pes) when these inhibitors were added before the start of the rna polymerase reaction. however, if rna synthesis was allowed to occur before ata or pes was added, rna synthesis continued for a short time (10 min or less) in the presence of either inhibitor at a concent ... | 1975 | 171445 |
| method of examining viral rna metabolism in cells in culture: metabolism of vesicular stomatitis virus rna. | a method is described for radioactively labeling viral rna and then quickly halting further incorporation of radioactive precursor into rna so that the fate of the labeled rna can be followed. small complementary rnas synthesized during vesicular stomatitis virus infection in the presence of cycloheximide do not metabolize to virion length molecules when protein synthesis inhibition is reversed. | 1975 | 171456 |
| translation in vitro of vesicular stomatitis virus mrna lacking 5'-terminal 7-methylguanosine. | the 5'-terminus 7-methylguanosine of vesicular stomatitis virus mrna is not essential for translation of the mrna. the 7-methylguanosine seems to have a mediating rather than an obligatory role in ribosome binding by the mrna and in mrna translation. | 1976 | 180425 |
| experimental vesicular stomatitis virus infection: ultrastructural pathology. | | 1975 | 172368 |
| studies on the asymmetric arrangement of membrane-lipid-enveloped virions as a model system. | lipids of bhk 21 cells (baby hamster kidney) grown in tissue culture were labelled with radioactive fatty acids. the enveloped vesicular stomatitis virus was propagated in this host cell type. the virions were purified by density gradient centrifugation. neuraminidase treatment of the intact virions led to a complete transformation of hematoside [n-acetylneuraminosyl(alpha2-3)lactosyl(beta1-1)ceramide] into lactosylceramide, with identical labelling of the ceramide portion in hematoside of the u ... | 1975 | 172423 |
| rescue of vesicular stomatitis virus from homologous and heterologous interferon-induced resistance in human cell cultures by poxviruses. | in human cell cultures the ability of poxviruses to rescue vesicular stomatitis virus from human interferon-induced resistance was significantly more efficient than the ability to rescue it from simian interferon-induced resistance. the sensitivity of the poxvirus to interferon was not related to its ability to rescue vesicular stomatitis virus. | 1976 | 181327 |
| inhibition of cellular protein synthesis by simultaneous pretreatment of host cells with fowl plague virus and actinomycin d: a method for studying early protein synthesis of several rna viruses. | a method is described for analysis of viral protein synthesis early after infection when minute amounts of viral proteins are effectively concealed by large amounts of produced host-specific proteins. the method is superior to a radioimmune assay, since all virus-induced proteins can be measured independent of their immunological reactivity. host-specific protein synthesis can be suppressed by infection with fowl plague virus. addition of actinomycin c 1.25 h postinfection does not prevent this ... | 1975 | 173875 |
| temperature-sensitive mutants of vesicular stomatitis virus are conditionally defective particles that interfere with and are rescued by wild-type virus. | temperature-sensitive (ts) mutants of vesicular stomatitis virus belonging to complementation groups i, ii and iv inhibited the replication of wild-type vesicular stomatitis virus when mixed infections were carried out in bhk21 cells at 32, 37, and 39.5 c. the group iv mutant (ts g 41) was most effective in this regard; wild-type virus yields were inhibited almost 1,000-fold in mixed infections with this mutant at 32 c. in the case of group i and ii mutants, inhibition of wild-type virus replica ... | 1976 | 181590 |
| differential inhibition of vesicular stomatitis virus polypeptide synthesis by hypertonic initiation block. | synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infected cells to hypertonic medium. it is concluded that the relative sensitivity of individual viral polypeptide synthesis to hypertonic initiation block is independent of the site of ... | 1975 | 173890 |
| role of temperature-sensitive mutants in persistent infections initiated with vesicular stomatitis virus. | noncytocidal persistent infections at 37 c of mouse l cells (lvsv) with infective b particles of vesicular stomatitis virus (vsv) could be established only in the presence of large numbers of defective interfering (di) particles. under these conditions, there was a rapid spontaneous selection of temperature-sensitive (ts) virus. at 10 days there was an increase to 17.8% in the frequency of ts clones in the virus population; by 17 days this frequency had reached 85.2%, and by 63 days 100% of the ... | 1976 | 181599 |
| mechanisms of vesicular stomatitis virus-induced cytopathic effects. i. early morphologic changes induced by infectious and defective-interfering particles. | | 1976 | 181906 |
| mechansims of vesicular stomatitis virus-induced cytopathic effects. ii. inhibition of macromolecular synthesis induced by infectious and defective-interfering particles. | | 1976 | 181907 |
| 5'-terminal sequence of vesicular stomatitis virus mrna's synthesized in vitro. | unmethylated or methylated 12 to 18s mrna's synthesized in vitro by the virion-associated rna polymerase of vesicular stomatitis virus contain the 5'-terminal hexanucleotide sequence g(5')ppp(5')apapcpapgp...or m 7g(5')-ppp(5')apmapcpapgp..., respectively. the implication of these results in relation to the regulation of transcription in vesicular stomatitis virus is discussed. | 1975 | 173891 |
| enveloped viruses as model membrane systems: microviscosity of vesicular stomatitis virus and host cell membranes. | the fluorescence probe 1,6-diphenyl-1,3,5-hexatriene was used to study and compare the dynamic properties of the hydrophobic region of vesicular stomatitis virus grown on l-929 cells, plasma membrane of l-929 cells prepared by two different methods, liposomes prepared from virus lipids and plasma membrane lipids, and intact l-929 cells. the rate of penetration of the probe into the hydrophobic region of the lipid bilayer was found to be much faster in the lipid vesicle bilayer as compared with t ... | 1976 | 182211 |
| [induction of dolykaryocytes by vesicular stomatitis virus in xc rat cells]. | v.s.v. induced polycaryocytes in rat embryonic fibroblasts, transformed by the prague strain of sarcoma rous (xc cells). this fusion is strictly dependent on the expression of the viral genome and is probably due to the incorporation of viral antigens in the cell membrane. the integrity of cellular rna synthesis is however not required. the fusion is probably due to a membrane structure characteristic of these transformed cells. | 1976 | 182411 |
| virus-dependent glycosylation. | the oligosaccharides of the membrane glycoproteins of sindbis virus, vesicular stomatitis virus, and rous sarcoma virus were compared on the basis of apparent size and sugar composition. it appears that each virus acquires a different set of oligosaccharides during growth in a single type of cell. | 1975 | 173894 |
| order of transcription of genes of vesicular stomatitis virus. | the effect of ultraviolet radiation on the expression of the genes of vesicular stomatitis virus (vsv) was studied in a cell-free system which executed coupled transcription and translation of the viral genes. in this system, which contained detergent-activated virus and a cytoplasmic extract of mouse l-cells, three of the five viral proteins (n, ns, and m) were synthesized accurately and efficiently; a putative precursor to the viral glycoprotein (g) was also made, but synthesis of the l protei ... | 1976 | 174107 |
| lethal synergy between sarcoma-180/tg cells and vesicular stomatitis virus in mice. | an interaction between sarcoma-180/tg cells and vesicular stomatitis virus in adult mice resulted in the rapid onset of extensive mortality. this interaction, termed lethal synergy, occurred only at early stages of ascites induction in animals with no prior virus contact. a significant sparing effect conferred by the serotonin antagonist dibenamine was reversed by the administration of serotonin. the cause of death was not determined, but a mechanism involving hypersensitivity is indicated. | 1975 | 174141 |
| inhibition of vesicular stomatitis virus replication by poly(2'-fluoro-2'-deoxyuridylic acid). | poly(2'-fluoro-2'-deoxyuridylic acid) is known to be an effective inhibitor of the deoxyribonucleic acid polymerase found within the oncornaviruses. this synthetic polynucleotide was found to inhibit the replication of vesicular stomatitis virus in mouse l cells. the polymer was shown to be capable of inhibiting the viral ribonucleic acid (rna)-dependent rna polymerase, and it is proposed that this is the mechanism of antiviral activity. the following observations support this viewpoint: (i) the ... | 1975 | 174488 |
| the size and the cistronic origin of defective vesicular stomatitis virus particle rnas in relation to homotypic and heterotypic interference. | | 1976 | 176367 |
| studies on the in vitro transcription and translation of vesicular stomatitis virus mrna. | | 1976 | 183350 |
| quantitative studies on adsorption, elution, and haemagglutination of vesicular stomatitis virus. | radioactively-labelled vsv, both indiana and new jersey serotypes, were rapidly and efficiently adsorbed onto goose erythrocytes, but only within a narrow range of ph and at low temperatures. under optimal conditions for adsorption, both infectivity and haemagglutinating activity of vsv were reduced. a basic difference between the two serotypes was identified: adsorption and haemagglutination of the indiana serotype were stable even at ph 9.0 and at 37 degrees c, whereas new jersey serotype was ... | 1976 | 183632 |
| ultrastructure of mouse periventricular and choroid plexus tissues in experimental vesicular stomatitis virus infection. | the ultrastructure of the periventricular and choroid plexus tissues was studied in the three-week-old mouse, infected with the vesicular stomatitis virus (vsv) by the intranasal route. the vsv reached the periventricular ependymal lining late in infection. it was carried within the cytoplasm of myelinated axons and their sheath cells. inflammatory cells, although few in numbers, may have also contributed to the virus dissemination. once in the periventricular tissues the vsv entered the cells f ... | 1976 | 183634 |
| characterization and translation of methylated and unmethylated vesicular stomatitis virus mrna synthesized in vitro by ribonucleoprotein particles from vesicular stomatitis virus-infected l cells. | ribonucleoprotein particles isolated from extracts of vesicular stomatitis virus (vsv) -infected l cells synthesized in vitro four classes of polyadenylated rna sedimenting at 29s, 19s, 17s, and 13s. when synthesized in vitro in the presence of the methyl donor s-adenosyl methionine, these rna species contained the following 5'-terminal structures: (i) m7g5ppp5'ampap(70%) ; (ii) m7g5'ppp5'ampampnp (20%) and (iii) pppap (10%). in the presence of the methylation inhibitor s-adenosylhomocysteine, h ... | 1976 | 176426 |
| factors involved in the generation and replication of rhabdovirus defective t particles. | previous indications that cloned b virions might be genetically predisposed to generate a particular defective t particle are shown to be inaccurate. t particle generation was found to be a much more random process than was previously believed. we show that the previously observed generation of particular sizes of t particles by b virion pools is due to the random generation of t particles during preparation of first-passage pools of cloned b virions, and these breed true during the additional p ... | 1976 | 176445 |
| assay of bovine interferons in cultures of the porcine cell line ib-rs-2. | an assay for bovine interferons has been developed using the porcine cell line ib-rs-2 and a bovine enterovirus, cbv-d, as challenge virus. the method is based on estimation of cytopathic effect measured by uptake of neutral red. teh assay is simple, sensitive, and reproducible. a comparative test of different viruses in ib-rs-2 cells and secondary calf kidney cells revealed that the sensitivity of a virus to interferon can vary up to 1,000-fold in the two cell systems. vesicular stomatitis viru ... | 1976 | 184049 |
| oligosaccharide moieties of the glycoprotein of vesicular stomatitis virus. | vesicular stomatitis virus contains a single structural glycoprotein whose carbohydrate sequences are probably specified by the host cell. the glycopeptides derived by pronase digestion of the glycoprotein of vesicular stomatitis virus grown in hela cells have an average molecular weight of 1,800. there are multiple oligosaccharide chains on the vesicular stomatitis virus glycoprotein with protein-carbohydrate linkages that are cleaved only by strong alkali under reducing conditions, suggesting ... | 1976 | 176458 |
| induction of murine p30 by superinfecting herpesviruses. | the interaction of endogenous type c viruses with superinfecting herpes simplex virus type 2 (hsv-2) was investigated in two murine cell lines. replication of hsv-2 was suboptimal in random-bred swiss/3t3a cells and, in initial experiments, infection with a low virus-to-cell ratio resulted in carrier cultures with enhanced murine leukemia virus (mulv) p30 expression. immunofluorescence tests with swiss/3t3a cells productively infected with hsv-2 also showed hsv-associated cytoplasmic antigens an ... | 1976 | 184296 |
| host restriction of friend leukemia virus coat protein synthesis. | fv-1 gene-mediated host restriction of friend leukemia virus replication was investigated in terms of coat protein synthesis. by using the assay of pseudotype formation with vesicular stomatitis virus. it was shown that under restricting growth conditions the availablity of leukemia virus coat protein for pseudotype formation was decreased. these studies appear to eliminate a pure assembly defect as the mechanism of fv-1 host restriction. | 1976 | 176469 |
| comparison of vesicular stomatitis virus defective interfering particle synthesis in chick embryo and l cells. | a comparison of the ability of vesicular stomatitis virus (vsv) to generate and replicate defective interfering (di) particles in primary chick embryo (ce) and mouse l cells was investigated as a means of analyzing host control over di-particle synthesis and interfering capacity. serial undiluted passage of vsv in ce and l cells indicate that vsv-di particles are generated and (or) replicate with greater efficiency in ce than in l cells. when di particles accumulate in l cells, they are able to ... | 1976 | 184896 |
| effect of membrane protein on lipid bilayer structure: a spin-label electron spin resonance study of vesicular stomatitis virus. | spin-label electron spin resonance (esr) methods have been used to study the structure of the envelope of vesicular stomatitis virus (vsv). the data indicate that the lipid is organized in a bilayer structure. proteolytic digestion of the glycoproteins which are the spike-like projections on the outer surface of the virus particle increases the fluidity of the lipid bilayer. since the lipid composition of the virion reflects the composition of the host plasma membrane and the protein composition ... | 1976 | 179567 |
| viral spread in the presence of neutralizing antibody: mechanisms of persistence in foamy virus infection. | several viruses were categorized on the basis of their ability to spread from cell to contiguous cell and form plaques in the presence of antiviral antibody. herpes simplex virus, cytomegalovirus, and vaccinia, measles, and foamy viruses were able to spread in the presence of neutralizing antibody, whereas coxsackievirus, encephalomyocarditis virus, vesicular stomatitis virus, mumps virus, and simian virus 5 failed to spread. a detailed study of one of these virus groups (simian foamy viruses) s ... | 1976 | 185150 |
| effect of neutral red and light on herpesvirus hominis type 1 in cell culture. | various concentrations of neutral red were added to monolayers of muscle-skin fibroblasts after adsorption of herpesvirus hominis type 1. the concentration necessary to reduce plaque counts was found to be 10(-5.5) m. at the same time, the minimal toxic concentration of neutral red for muscle-skin fibroblasts was determined by the concentration that reduced the plaques of a challenge virus, vesicular stomatitis virus, that was applied after treatment with neutral red and light. the minimal toxic ... | 1976 | 185301 |
| function and structure of rna polymerase from vesicular stomatitis virus. | the rna-dependent rna polymerase associated with vesicular stomatitis virus was isolated to apparent homogeneity by a newly developed procedure, which includes stepwise removal of proteins from virions by successive treatment with high concentrations of cesium sulfate and cesium chloride, followed by glycerol gradient centrifugation or chromatography on phosphocellulose or deae-sephadex column. the polymerase thus purified contained l (large protein) and ns proteins as the intrinsic subunits and ... | 1976 | 180023 |
| persistence of the viral genome in interferon-treated cells infected with oncogneic or nononcogenic viruses. | in akr mouse cells chronically infected with a murine leukemia virus, treatment with interferon for nine days resulted in sustained inhibition of extracellular production of murine leukemia virus but no inhibition of viral intracellular p30 antigen or of reverse transcriptase. removal of interferon resulted in rapid reversal of these effects. interferon-treated mouse l-cells were infected with high multiplicities of vesicular stomatitis virus or herpes simplex virus type 1. infectious virus and ... | 1976 | 180209 |
| synthesis of rna by mutants of vesicular stomatitis virus (indiana serotype) and the ability of wild-type vsv new jersey to complement the vsv indiana ts g i-114 transcription defect. | the ability of certain vesicular stomatitis virus (vsv; indiana serotype) temperature-sensitive (ts) mutants to synthesize intracellular viral complementary rna (vcrna) at permissive or nonpermissive temperatures for productive infections has been investigated. mutants belonging to complementation groups ii, iii, and v synthesize rna at nonpermissive temperature in amounts essentially equivalent to that obtained at permissive temperatures. mutant ts g i-114 possesses a thermolabile transcriptase ... | 1976 | 185410 |
| enhanced growth and plaquing of rabies virus in static chick embryo cell culture. | the 7-day egg passage line of hep flury strain of rabies virus was inoculated to primary chick embyro (ce) cells prepared in different ways to compared efficiencies of viral growth and plaquing. special care to minimize cellular damage due to trypsin at the step of monodispersion and sowing a comparatively large number of cells for monolayer preparation were required for rabies plaquing, whereas such cares were not necessary for plaquing of vesicular stomatitis virus. plaque number and size were ... | 1976 | 185442 |
| human fibroblast and leukocyte interferons show different dose-response curves in assay of cell protection. | preparations of human fibroblast and leukocyte interferons of similar potency show markedly different dose-response curves in an assay which measures the degree of protection of tissue cultures against virus c.p.e. the effect is observed with both vesicular stomatitis virus (vsv) and mengovirus, and is not altered by purification of the interferons. | 1976 | 180244 |
| specific inactivation of herpes simplex virus by silver nitrate at low concentrations and biological activities of the inactivated virus. | the infectivities of herpes simplex virus types 1 and 2 were inactivated by silver nitrate at concentrations of 30 mum or less, which did not affect at all the infectivities of hemagglutinating virus of japan, vesicular stomatitis virus, poliovirus, vaccinia virus, and adenovirus. the inactivated virus retained the capability of adsorbing to the cell, with an adsorption kinetics quite similar to that of intact virus, and of inducing the concanavalin a agglutinability in the infected cells, where ... | 1976 | 185946 |
| ultrastructural changes in bovine lingual epithelium infected with vesicular stomatitis virus. | vesicular stomatitis virus was inoculated into the dorsal lingual epithelium of three cows. the reaction that developed in 72 h was characterized by severe acute diffuse glossitis with intercellular edema and necrosis of keratinocytes. virions budded from the plasma membrane and were in the intercellular spaces. reduplication of desmosomes was a prominent alteration, and normal desmosomes were within the cytoplasm. intracytoplasmic desmosomes appeared to be formed by endocytosis after breaks occ ... | 1975 | 180646 |
| decreased interferon production by leukocytes in marasmus. | interferon production by leukocytes in culture was investigated in nine severely marasmic infants and 31 well-nourished controls. the production of interferon was induced with newcastle disease virus and assayed in vero cells challenged with vesicular stomatitis virus. marasmic infants produced significantly less interferon than controls. it is suggested that the finding may be the result of a lymphocyte defect induced by malnutrition and could help to explain the increased frequency and severit ... | 1976 | 180790 |
| phenotypic mixing of vesicular stomatitis virus and d-type oncornavirus. | after mixed infection of cells with vesicular stomatitis virus (vsv, thermolabile mutant tl 17) and oncornavirus type d, phenotypically mixed virions are formed containing the vsv genome and oncornavirus and vsv envelope. the virions are thermostable and have serologic characteristics of both viruses. | 1976 | 181332 |
| comparative membrane microviscosity of fish and mammalian rhabdoviruses studied by fluorescence depolarization. | the microviscosity of the hydrophobic region of the membrane of infectious hematopoietic necrosis virus was determined using fluorescence depolarization analysis of the probe 1,6-diphenyl-1,3,5-hexatriene and was found to be much lower at 37 c than that of another rhabdovirus, vesicular stomatitis virus. however, the microviscosity of this fish virus at 18 c, the temperature at which it was grown, corresponded to the microviscosity of vesicular stomatitis virus at 37 c. data obtained with the fi ... | 1976 | 181597 |
| association of vesicular stomatitis virus proteins with hela cell membranes and released virus. | the association of vesicular stomatitis virus proteins with intracellular and plasma membranes was examined by pulse and pulse-chase labeling of virus-infected hela cells with [35s]methionine and separation of cell homogenates into three major membrane fractions in discontinuous sucrose gradients. the glycoprotein g was primarily associated with rough endoplasmic reticulum-like membranes after short radioactive pulses (2 to 4 min) but accumulated in the plasma membrane-enriched fraction and the ... | 1976 | 186639 |
| glycosylation of vesicular stomatitis virus glycoprotein in virus-infected hela cells. | glycosylation of the envelope glycoprotein of vesicular stomatitis virus was examined using virus-infected hela cells that were pulse-labeled with radioactive sugar precursors. the intracellular sites of glycosylation and the stepwise elongation of the carbohydrate side chains of the g protein were monitored by membrane fractionation and gel filtration of pronase-digested glycopeptides. the results with short pulses of sugar label (5 to 10 mtein linkage (glucosamine and mannose) are added to g w ... | 1976 | 186640 |
| asymmetric distribution of phosphatidylethanolamine in the membrane of vesicular stomatitis virus. | the membrane-impermeable reagent trinitrobenzenesulfonate has been shown to react only with the surface components of vesicular stomatitis virus (vsv) membranes. when the amount of phosphatidylethanolamine (pe) available to modification by trinitrobenzenesulfonate in intact virions was determined, it was found that 36% of the total membrane pe was converted to the trinitrophenyl derivative. the same proportion of the total membrane pe was reactive after removal of the surface glycoprotein by try ... | 1976 | 186641 |
| status spongiousus resulting from intracerebral infection of mice with temperature-sensitive mutants of vesicular stomatitis virus. | mice infected intracerebrally (i.c.) with wild-type (wt) vsv or temperature-sensitive (ts) mutants, ts 11, ts 22, ts 31 and ts 41, were studied for the development of histopathological lesions in the central nervous system (cns). mice infected i.c. with wt vsv exhibited histopathological lesions consisting principally of occasional foci of perivascular mononuclear cell infiltration and rare foci of necrosis. all wt vsv infected mice died within 2 days of i.c. inoculation. in contrast, mice infec ... | 1976 | 182197 |
| augmented immunogenicity of tumor cell membranes produced by surface budding viruses: parameters of optimal immunization. | membranes prepared from tumor cells infected with surface budding viruses are much more immunogenic than membranes from uninfected tumor cells. factors affecting immunization with membranes from virus-infected tumor cells were studied. preparations made with influenza virus were clearly superior to those prepared with vesicular stomatitis virus (vsv). membranes infected with vsv were maximally immunogenic at a dose equivalent to a 10% cell pack whereas influenza-virus-infected membranes were imm ... | 1976 | 182646 |
| multiplication of vesicular stomatitis virus in the leafhopper peregrinus maidis (ashm.), a vector of a plant rhabdovirus. | vesicular stomatitis virus (vsv) was found to multiply efficiently in whole peregrinus maidis (ashm.). the leafhopper vector of maize mosaic virus (mmv), a plant rhabdovirus. insects were inoculated with vsv by means of a microsyringe, collected at 1-day intervals and tested individually for the presence of virus. exponential virus multiplication occurred within the first 4 days, reaching titres of 10(6) p.f.u. per insect in days 5 to 10 after inoculation. these observations show that a common h ... | 1976 | 182911 |
| analysis of in vitro transcription products of intracellular vesicular stomatitis virus rna polymerase. | the intracellular transcriptase complex of vesicular stomatitis virus-infected l cells synthesized rna complementary to the entire infectious virus genome at either 37 degrees c or 28 degrees c in vitro. not all sequences were present at the same frequency, however; copies of that segment of the genome common to the lt defective particles were present at 20 to 100 times higher frequently than copies of the genome segment common to the st defective particle. the less frequent region was transcrib ... | 1976 | 183012 |
| biological and electron microscopic studies on the phenotypic mixing of the thermolabile mutant of vesicular stomatitis virus, tl-17, with avian rna tumor viruses. | a thermolabile as well as thermosensitive mutant of vesicular stomatitis virus, vsv-tl-17, could be thermostabilized by phenotypic mixing with avian rna tumor viruses (atv). biological, immunological and morphological studies revealed that this effect is due to a replacement of the thermolabile projections of the vsv by the avian tumor viral projections. the arrangement of projections of vsv and atv on phenotypically mixed viria was studied by electron microscopy. a-type particles were detected ... | 1976 | 187154 |
| the pathogenesis of vesicular stomatitis virus, serotype indiana, in aedes aegypti mosquitoes, after imbibition of a viremic blood meal. | this study showed that vesicular stomatitis virus (indiana) in most instances was not capable of replicating in aedes aegypti when imbibed by the mosquitoes on a viremic host. rapid inactivation of the virus was observed in some cases within 24 hours after imbibition. attempts to demonstrate virus inactivation by midgut contents in vitro were not successful. | 1976 | 187155 |
| translation of individual host mrna's in mpc-11 cells is differentially suppressed after infection by vesicular stomatitis virus. | infection of mpc-11 mouse plasmacytoma cells by vesicular stomatitis virus results in 30 to 35% reduction in [35s]methionine incorporation into total proteins within 30 min postinfection. by 6 h postinfection, total protein synthesis is reduced by 80 to 90%. however, even by 30 min postinfection, a differential suppression of the synthesis of individual host protein is observed. the synthesis of the immunoglobin g (igg) heavy chain (h), and, in particular, the synthesis of igg light chain (l), i ... | 1976 | 183015 |
| an ultrastructural study of central nervous system disease produced by wild-type and temperature-sensitive mutants of vesicular stomatitis virus. | outbred swiss mice 3 to 4 weeks of age were injected intracerebrally with wild-type vesicular stomatitis virus or its temperature-sensitive (ts) mutants ts 11, ts 22, ts 31, and ts 41. brain and spinal cord were then studied for pathologic changes by electron microscopy. all mice infected with wild-type vesicular stomatitis virus died within 2 days of inoculation. diffuse ependymal alterations often culminating in necrosis in brain and especially spinal cord and rare foci of necrosis and mononuc ... | 1976 | 183052 |
| 3'-terminal sequence of vesicular stomatitis virus genome rna. | | 1976 | 178307 |
| ecological associations of vesicular stomatitis virus in rural central america and panama. | the principal objective of this study was to determine ecological associations of vesicular stomatitis virus (vsv)- new jersey and vsv-indiana in rural central america and panama. two types of information were linked: the results of neutralizing antibody tests performed on sera from 3,232 lifetime residents of 189 rural study communitities of central america and panama, and ecological characteristics of the study communities as determined from natural resource atlases. the major finding was that ... | 1976 | 188350 |
| determination of molar ratios of vesicular stomatitis virus induced rna species in bhk21 cells. | a modified procedure for analysis of rna in denaturing formamide-polyacrylamide slab gels containing 6 m urea is described. using this technique, in conjucntion with fluorographic analysis, we determined molecular weights and molar ratios of the various vesicular stomatitis virus (vsv) induced rnas in bhk21 cells. a comparison of the molar ratios of virus-specific mrhas and their putative protein products in these cells suggests that there is little, if any, translational control of viral gene e ... | 1976 | 178352 |
| vesicular stomatitis virus: mode of transcription. | recent studies on the mechanism by which the virion-associated rna polymerase of vesicular stomatitis virus transcribes rna have revealed several new biological features of general interest. the mode of synthesis of the 5'-terminal cap structure of the mrnas, the sequential transcription of the genes and the presence of a transcribed "leader" rna segment are properties which are either not shown by other viruses, or have not yet been described. these features are probably inter-related with the ... | 1977 | 188975 |
| characterization of a virion protein kinase as a virus-specified enzyme. | antibodies which completely inhibited the enzymatic activity of the protein kinase associated with virions of frog virus were obtained by immunization of rabbits with the purified enzyme. this inhibition provided a specific probe for the frog virus protein kinase, since this antiserum had no inhibitory effect on a variety of other protein kinases, including the activity of uninfected cells, or the protein kinase associated with vesicular stomatitis virus or vaccinia virus cultivated in the same ... | 1976 | 178663 |
| effects of temperature and antibody on the cyclid growth of vesicular stomatitis virus. | to see the effects of temperature on the interrelated cyclic production of standard and defective interfering (di) particles of vesicular stomatitis virus, a temperature-sensitive (ts) g114 mutant was passaged successively at different temperatures and the production of the two types of viral particles as well as the ability of chinese hamster ovary cells to survive each passage was continuously monitored. when the temperature was nonpermissive for standard virus, the synthesis of both standard ... | 1977 | 189064 |
| assembly of vesicular stomatitis virus glycoprotein and matrix protein into hela cell plasma membranes. | | 1976 | 178880 |
| differential inhibition of host protein synthesis in l cells infected with rna - temperature-sensitive mutants of vesicular stomatitis virus. | the response of mouse l cells to infection with wild-type (wt) and temperature-sensitive (ts) mutants of vesicular stomatitis virus was monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to delineate the synthesis of host cell and viral proteins. experiments utilized transcriptase mutants of complementation group i (ts114 and ts13), a group iv mutant (ts44) that is restricted in total rna synthesis (rna-1) but not in primary transcription, and a group ii mutant (ts52) variabl ... | 1976 | 178896 |
| transcriptional mapping of vesicular stomatitis virus in vivo. | synthesis of the proteins of vesicular stomatitis virus (indiana serotype) was studied in mouse l cells infected with virus that had been exposed to uv radiation. the uv target sizes measured during primary transcription indicated that the five genes occupy a single transcriptional unit. thus, in infected cells, as a cell-free system, transcription of vesicular stomatitis virus rna initiates at a single point and proceeds in the order n. ns, m, g, and l. | 1977 | 189074 |
| sequential transcription of the genes of vesicular stomatitis virus. | increasing exposure of vesicular stomatitis virus particles to ultraviolet radiation caused differential inhibition of the synthesis in vitro of individual mrna species which code for the viral structural proteins l, g, m, ns, and n. | 1976 | 179088 |
| assembly of viral membranes. i. association of vesicular stomatitis virus membrane proteins and membranes in a cell-free system. | we report here an in vitro system designed to study the interactions of vesicular stomatitis virus (vsv) proteins with cellular membranes. we have synthesized the vsv nucleocapsid (n) protein, nonstructural (ns) protein, glycoprotein (g protein), and membrane (m) protein in a wheat germ, cell-free, protein-synthesizing system directed by vsv 12 to 18s rna. when incubated at low salt concentrations with purified cytoplasmic membranes derived from chinese hamster ovary cells, the vsv m andg protei ... | 1977 | 189082 |
| analysis of the rna species isolated from defective particles of vesicular stomatitis virus. | serial high multiplicity passage of a cloned stock of vesicular stomatitis virus was found to generate defective interfering particles containing three size classes of rna, with sedimentaiton coefficients of 31 s, 23 s and 19 s. the 31 s and 23 s rna species were found to be complementary to both the 12 to 18 s and 31 s size classes of vsv mrnas. the 19 s class of rna was found to be partially base-paired. all three rna species were found to contain ppap at their 5' termini. | 1976 | 185328 |
| effects of interferon on cell and virus growth in transformed human cell lines. | the anticellular and antiviral effects of human leukocyte interferons were studied in vitro in the transformed human embryonic cell lines. rsa and rsb. the growth of these cells was inhibited and they began to deteriorate about 48 h after treatment with 500 units/ml of interferon. when interferon was washed out within 48 h, their growth recovered gradually. the effects of interferon on cell growth depended on the amount of interferon added per cell. a subline, named ifr, was isolated which grows ... | 1976 | 185329 |
| [mechanism of resistance of a clone isolated from l cells chronically infected with the virus of vesicular stomatitis]. | the results of the study on antiviral immunity acquired by l cells to vesicular stomatitis virus are presented. we failed to detect the presence of the indicator virus in the resistant culture by means of virological, electron microscopic or cytochemical methods. molecular hybrization experiments demonstrated the lack in the nuclear dna of sequences homologous to vesicular stomatitis virus rna. | 1976 | 185809 |
| 13c-nmr studies of the membrane structure of enveloped virions (vesicular stomatitis virus). | the mobility of the lipids in the bilayer of the envelope of vesicular stomatitis virus has been probed over its complete space by the biosynthetic incorporation of [n-13ch3]- choline as a probe for the polar head groups and [3-13c]- and [11-13c] oleic acid and [16-13c]- palmitic acid for the hydrophobic region of the bilayer. these precursors were effectively incorporated as established by the concomitant administration of the same precursors in radioactive form. spin lattice relaxation time me ... | 1976 | 186376 |
| long-term persistent vesicular stomatitis virus and rabies virus infection of cells in vitro. | bhk 21 carrier cells persistently infected with vsv indiana for over 2 years have been shedding generally very low levels of mature infectious virus or mature t particles (averaging less than one-hundredth p.f.u./cell/day) yet most cells are producing virus antigens and are resistant to homologous superinfection. however, large amounts of biologically active t particle rnp can be recovered from cytoplasmic extracts of these carrier cells even at times when they are shedding no detectable infecti ... | 1976 | 186559 |
| rna synthesis in bhk 21 cells persistently infected with vesicular stomatitis virus and rabies virus. | virus-induced rna synthesis was studied in bhk 21 cells persistently infected with vesicular stomatitis virus (vsv) and rabies virus by labelling rna synthesized in the presence of antinomycin d. during persistent infection the species of messenger rna synthesized were similar in size and relative proportions to those seen during acute infection, but there were some minor differences. full-sized b virion rna was generally not detected during persistent infection, and new species (probably di vir ... | 1976 | 186560 |
| homologous interference induced by a temperature-sensitive mutant derived from an hvj (sendai virus) carrier culture. | homologous interference between a temperature-sensitive small plaque mutant (hvj-pb) derived from an hvj (haemagglutinating virus of japan - the sendai strain of parainfluenza i virus) carrier culture of bhk cells and the original wild-type virus (hvj-w) has been investigated. prior infection of llcmk2, hela, bhk or mouse l cells with hvj-pb, both at permissive and non-permissive temperatures, for 24 h resulted in a reduced yield of superinfecting hvj-w, reflecting a smaller number of cells capa ... | 1976 | 186564 |
| in vivo assembly and maturation of vesicular stomatitis virus. | previous studies on vesicular stomatitis virus (vsv) maturation in infected cells have utilized in vitro cell cultures. the present study is, to our knowledge, the first in vivo analysis of vsv-cell interaction in the central nervous system of weaning outbred swiss mice. intracerebral inoculation of wild-type vsv resulted in rapid viral replication in brain and spinal cord. by immunoflourescence, viral antigens were first seen in ependymal cells of brain and spinal cord and soon thereafter in su ... | 1976 | 186663 |
| pseudotypes of avian sarcoma viruses with the envelope properties of vesicular stomatitis virus. | | 1977 | 190775 |
| rna synthesis in standard and autointerfered vesicular stomatitis virus infections. | | 1977 | 190781 |
| mapping and initiation studies on the leader rna of vesicular stomatitis virus. | | 1977 | 190783 |
| action spectra for the rescue of temperature-sensitive mutants of vesicular stomatitis virus by ultraviolet-irradiated virions at nonpermissive temperature. | | 1977 | 190784 |
| [effect of actinomyces rimosus ribonuclease on the reproduction of viruses]. | antiviral activity of rna-ase isolated from the fermentation broth of actinomyces rimosus was studied. the effect of the enzyme on multiplication of the viruses of vesicular stomatitis, newcastle and cariolovaccine diseases was investigated. it was found that the enzyme was capable of suppressing reproduction of the vesicular stomatitis virus (vsv) in the culture of chick fibroblast cells. the suppression level directly depended on the enzyme concentration and decreased with an increase in the i ... | 1977 | 190944 |
| functional and ultrastructural studies of the effects of human interferon on cell membranes of in vitro cultured cells. | the effect of human leukocyte interferon on cultured u-amnion cells was examined, and several biological parameters were registered. multiplication of vesicular stomatitis virus and the virus-produced cytopathogenic effect was prevented. the growth rate of uninfected cells was reduced, as well as the spontaneous release of 3h-uridine. these effects were observed following treatment with 10 units of interferon per ml. no morphological alterations could be detected by scanning electron microscopy ... | 1976 | 187008 |
| cell-free translation of rna synthesized in vitro by a transcribing nucleoprotein complex prepared from purified vesicular stomatitis virus. | the rna species synthesized in vitro by a transcribing nucleoprotein (tnp) complex of vesicular stomatitis virus (vsv) were translated with high efficiency in a fractionated cell-free system derived from reticulocytes. the use of tnp complexes isolated from vsv indiana, vsv new jersey, and chandipura viruses showed that in each case the predominant polypeptides synthesized had electrophoretic mobilities identical to their virion n, ns, and m polypeptides in proportions reflecting those found in ... | 1977 | 191633 |
| in vitro interferon production by bovine tissues: effects of hydrocortisone. | the effect of hydrocortisone on bovine interferon production in vitro was studied. infectious bovine rhinotracheitis virus was used as an inducer. interferon was assayed by the plaque-reduction method in bovine fetal kidney cultures, using vesicular stomatitis virus as challenge virus. hydrocortisone decreased interferon production in bovine fetal spleen and peripheral blood leukocyte cultures. hydrocortisone did not decrease interferon production by bovine alveolar macrophages, in 1 experiment. ... | 1976 | 187092 |
| in vitro interferon production by bovine tissues: induction with infectious bovine rhinotracheitis virus. | the interferon-inducing ability of infectious bovine rhinotracheitis (ibr) virus was determined in tissue cultures of bovine origin inoculated with untreated and ultraviolet (uv) irradiated ibr viruses. interferon was assayed by the plaque-reduction method in bovine fetal kidney (bfk) cell cultures, using vesicular stomatitis virus as challenge virus. highest interferon concentrations were produced by cultures of bovine fetal (bf) spleen cells and aveolar macrophage cultures derived from adult c ... | 1976 | 187093 |
| vesicular stomatitis virus plaque production in monolayer cultures with liquid overlay medium: description and adaptation to a one-day, human interferon-plaque. | vesicular stomatitis virus forms discrete, microscopic plaques in stationary cultures of the wish amnion cell line. microplaque formation is rapid, reproducible, and easily quantitated, occurs at temperatures ranging from 33 to 40 degrees c, and does not require a semisolid overlay. wish cells, however, are less sensitive to vesicular stomatitis virus than are chicken embryo, 3t6, or vero cells. wish amnion cells also are highly sensitive to the antiviral effects of human interferon, and a quant ... | 1976 | 187619 |
| effect of selective complement deficiency on the rate of neutralization of enveloped viruses by human sera. | the capacity of human sera genetically deficient in selective complement (c) components to enhance neutralization of enveloped viruses was examined by kinetic plaque reduction assays. vaccinia virus, a dna virus, and vesicular stomatitis virus (vsv), an rna virus, were studied. exogenous rabbit: or human antibody to vaccinia virus, and guinea pig or human antibody to vsv were provided in limiting, c-dependent concentrations. igg antibodies predominated in most of the antisera employed. c5-defici ... | 1977 | 187701 |