preparation and characterization of monoclonal antibodies to nucleocapsid protein n and h glycoprotein of rinderpest virus. | fifteen stable mouse spleen cell myeloma hybrids (hybridomas) producing monoclonal antibodies to rinderpest virus proteins were produced. the specificity of these monoclonal antibodies was established by radioimmunoprecipitation followed by polyacrylamide gel analysis and immunofluorescence. nine antibodies were specific for the surface glycoprotein h. all the nine clones showed inhibition of haemagglutination by measles virus. the antibodies from two clones (a7d2 and b2f6) neutralise infectious ... | 1989 | 2471370 |
prevalence of rinderpest antibodies in sheep and goats in southern india. | rinderpest antibodies were demonstrated in 37 per cent of 2400 sheep and 29.5 per cent of 1000 goats in the southern states of india by the avidin-biotin enzyme linked immunosorbent assay technique. this technique, besides quantifying rinderpest antibodies, has provided a simple method for the differentiation of positive and negative serum samples on the basis of the development of a pink colour, a great advantage in the screening of large numbers of sera for field epidemiological studies. the p ... | 1988 | 3212917 |
economic analysis of the development and usage of thermoresistant rinderpest vaccine. | | 1988 | 3232645 |
structural proteins of rinderpest virus. ii: purification and characterization of the nucleocapsid protein n, the haemagglutinin protein, h and the matrix protein, m. | | 1988 | 3255671 |
the suitability of different microtitre plates for detection of antibody to virus antigens by indirect elisa. | to optimize enzyme linked immunosorbent assays (elisas) for the detection of virus-specific antibodies, a range of commercially available microtitre plates was evaluated for their ability to bind virus antigen. rinderpest virus and foot-and-mouth disease virus were investigated as target antigens. binding capacity for antigen, binding ratios (attachment of specific antibody versus that of non-immune antibody) and the variation in the results of the tests within and between plates were measured. ... | 1989 | 2541132 |
assay sensitivity and differentiation of monoclonal antibody specificity in elisa with different coating buffers. | buffers of different ph and ionic strength were employed as coating buffers for antigen adsorption to microtitre plates. their efficiency for coating plates with rinderpest virus (rpv) and foot-and-mouth disease virus (fmdv) antigens was studied by elisa with polyclonal and monoclonal antibody preparations. while the adsorption and detection of rpv antigen with polyclonal antiserum was highly dependent on the ionic strength and ph of coating buffer, adsorption of antigenically active fmdv antige ... | 1989 | 2551906 |
[evaluation of serological protection against cattle rinderpest of bovine livestock in chad]. | 2,544 bovine sera, sampled during the year 1986 either in the course of five field missions or at farcha's abattoir, have been studied by seroneutralization against rinderpest. this survey permits the determination of the overall rinderpest immunity rate in chadian cattle. | 1989 | 2626567 |
surveillance and monitoring programmes in the control of rinderpest: a review. | | 1989 | 2665257 |
temporary breakdown of immunological tolerance to dsdna and nucleohistone antigens in rabbits infected with rinderpest virus. | the rabbit-passaged l strain of rinderpest virus (rv) causes the transient induction of anti-nuclear antibodies (ana) in rabbits. it has been shown by an indirect immunofluorescence test that the target antigens of these ana are dna and/or dna-histone complexes (nucleohistone). here detailed examinations of the target antigens were carried out by elisa, and it was revealed that rabbit sera contained three types of antibodies: antibodies reacting equally with both dsdna and ssdna; those reacting ... | 1990 | 2265490 |
single and combined rinderpest vaccines. | | 1987 | 3314100 |
segment 5 of the rice dwarf virus genome encodes a protein highly conserved within the phytoreoviruses. | the complete nucleotide sequence of segment 5 (s5) of the rice dwarf virus (rdv) genome was determined. rdv s5 is 2571 bp in length and has a single long open reading frame which encodes a polypeptide of 801 amino acids (mr 90495). when compared to the wound tumor virus genome s5, there was 56.9% and 52.8% similarity in the nucleotide and amino acid sequences, respectively. this high similarity suggests that the s5 proteins of these viruses are functionally similar. | 1990 | 2273396 |
immunohistological localisation of rinderpest virus in formalin-fixed, paraffin-embedded tissues from experimentally infected cattle. | six freesian steers were subcutaneously inoculated with the virulent rinderpest virus strain kabete "0" and sacrificed at the height of fever. sections of formalin-fixed, paraffin-embedded tissues were stained according to the peroxidase anti-peroxidase (pap) technique. labelling of viral antigen, both in the cytoplasm and in the nuclei of infected cells, was observed in the epithelial cells of the upper and lower alimentary tract and in lymphoid organs, i.e. spleen, lymph nodes, pharyngeal tons ... | 1989 | 2669428 |
bovine interleukin 2: production kinetics in normal and in vivo antigen-primed cattle. | conditions influencing production kinetics of bovine interleukin 2 (il-2), viz. cell concentration, mitogen and its concentration, length of incubation, nutrient medium and in vivo antigen-priming were investigated. peripheral blood mononuclear cells (pbl) of outbred cattle of different age groups showed considerable variation in their ability to secrete il-2 which possibly reflects their immune competence. of the cultures initiated with pbl, 5 x 10(6) cells/ml cultured in serum free iscove's me ... | 1990 | 2279762 |
dot enzyme immunoassay for visual detection of peste-des-petits-ruminants virus antigen from infected caprine tissues. | an enzyme-linked immunosorbent microassay using nitrocellulose paper as the solid-phase support was developed for the detection of peste-des-petits-ruminants virus antigens in infected caprine tissue homogenates. dots of tissue homogenates were applied to nitrocellulose papers, and any unreacted sites were blocked with 5% skim milk powder in triethanolamine-buffered saline. after incubation of the papers in tissue culture supernatant monoclonal antibody against the peste-des-petits-ruminants vir ... | 1989 | 2674200 |
comparison of the effect of various chemical stabilizers and lyophilization cycles on the thermostability of a vero cell-adapted rinderpest vaccine. | the thermostability of a rinderpest vaccine produced on vero cells was evaluated using a variety of chemical stabilizers and lyophilization protocols. three stabilizer preparations and three lyophilization schedules were examined using accelerated stability testing at 37 degrees c. the vaccine preparation exhibiting the greatest stability at 37 degrees c was tested at three additional temperatures, 42, 45 and 56 degrees c, and an arrhenius plot was constructed from the data. the stability of the ... | 1990 | 2305545 |
detection of phocine distemper virus using the polymerase chain reaction. | during the fatal seal epizootics in the north and baltic seas in summer 1988 a virus was isolated which was shown to be the causal agent. it was subsequently classified as morbillivirus by neutralization assays, reaction with monoclonal antibodies and nucleic acid hybridization studies. the virus (tentatively called phocine distemper virus, pdv) is difficult to grow in culture making rapid diagnosis difficult. we have used the polymerase chain reaction (pcr) as an alternative and fast method to ... | 1990 | 2311537 |
[successful development and methods of use of rinderpest vaccine]. | | 1987 | 3442452 |
nucleotide sequence of rice dwarf virus genome segment 9. | the complete nucleotide sequence of the phytoreovirus rice dwarf virus (rdv) genome segment 9 is presented. it consisted of 1305 nucleotides and had an open reading frame that codes for a putative polypeptide of 351 amino acids. mr of the protein was calculated to be 38,598. the terminal nucleotides 5' gguaaa--gau 3' were the same as those of rdv genome segment 10. the fourth nucleotide from the 3' end of an expected conserved sequence was a c rather than the u found in the previously sequenced ... | 1989 | 2732716 |
preliminary observations on rinderpest in pregnant cattle. | a kabete 'o' strain of rinderpest virus enhanced in virulence was inoculated subcutaneously into four cows which were between six and eight months pregnant. all the cows developed clinical signs of rinderpest from the third day after inoculation and shed high titres of virus in their ocular and vaginal secretions during the course of the clinical disease. three of the cows died of rinderpest on the third day after the onset of fever but no virus was isolated from their fetuses which were examine ... | 1989 | 2750030 |
duration of maternal immunity to peste des petits ruminants. | | 1989 | 2773199 |
development of heat-stable recombinant rinderpest vaccine. | recombinant vaccinia virus (rvv) containing the full-length cdna of rinderpest virus (rv)-haemagglutinin (h) gene was constructed. the h gene was inserted into the attenuated vaccine strain of vaccinia virus (vv), le 16 m0, with two different promoters, namely cowpox virus a-type inclusion body (ati) promoter or vv 7.5 kilodalton (p7.5) promoter. these rvvs produced the same sized fully glycosylated rv-h protein in rk 13 cells as that of the authentic rv-h. their heat stability in the lyophylize ... | 1989 | 2818208 |
growth of rinderpest and bovine virus diarrhoea viruses in theileria parva infected lymphoblastoid cell lines. | | 1988 | 2844014 |
production of monoclonal antibodies against rice dwarf virus and characterization of antigenic proteins with affinity media. | hybridomas were developed which secreted antibodies to the core and outer capsids of rice dwarf virus (rdv). the reactive proteins of rdv were simply and rapidly analyzed using monoclonal antibody (moab)-coupled affi-gel 701 beads and sds polyacrylamide gel electrophoresis and were isolated with a moab-coupled sepharose 4b affinity column. | 1987 | 3654899 |
[serologic survey of rinderpest, ibr-ipv, rsb, pi3 and bvd-md viruses in cattle in shaba and western zaire]. | | 1988 | 2851152 |
detection of rinderpest antigen by latex agglutination test. | a slightly modified latex agglutination test was applied for detection of rinderpest antigen. the antigen was added to sensitized latex particles in the presence of hyperimmune antiserum to facilitate agglutination. out of 129 samples tested by latex agglutination (la), solid phase aggregation of coated erythrocytes (space), reverse phase passive haemagglutination (rpha) and counter immunoelectrophoresis (cie) test, 86.0, 86.8, 84.4 and 79.8 per cent, respectively, were found positive. | 1988 | 2902777 |
development and stability of rinderpest virus antigens in cattle tears and lymph nodes. | diffusible rinderpest virus antigens were demonstrated in increasing quantities in ocular and lymph node biopsies from rinderpest-infected cattle using agar gel immunodiffusion (agid) and counterimmunoelectrophoresis (ciep) tests. positive samples were detected from the second day of pyrexia to two days after death. the antigens in ocular secretions and lymph nodes were thermolabile being destroyed within five minutes at 56 degrees c and within two weeks at 4 degrees c. | 1986 | 3705176 |
characterization of antinuclear antibodies induced in rabbits by rinderpest virus infection. | rabbits infected with the l strain of rinderpest virus (rv) produced high titres of antinuclear antibodies (ana) which reached a maximum two weeks after inoculation but rapidly disappeared by 6-8 weeks. these anas reacted with hela cells by indirect immunofluorescence test resulting in a homogeneous nuclear fluorescence. in order to investigate the target antigens of anas, the effects on the nuclear fluorescence pattern of pretreating hela cells were examined: dnase 1 treatment resulted in a dec ... | 1988 | 3058091 |
the differential growth of virulent and avirulent strains of rinderpest virus in bovine lymphocytes and macrophages. | rinderpest virus (rv) grew readily in cultures of purified bovine peripheral blood lymphocytes and udder macrophages. the growth of three strains of rv was compared and there appeared to be a relationship between increasing virulence and increased ability to infect lymphocytes and macrophages. the proportion of infected cells as determined by the presence of virus antigens was a better indicator of affinity between a strain and cell type than production of new infectious virus. rv grew better in ... | 1985 | 3873515 |
development and distribution of rinderpest virus antigen in experimentally infected goats. | three goats, experimentally infected with rinderpest virus were examined for the development and distribution of precipitating antigens in various tissues and secretions using the agar gel immunodiffusion test. virus antigens were detected in ocular secretions and lymph node biopsies from the second to the fourth and fifth days of pyrexia, respectively, but were not detected in nasal secretions. precipitating antigens were demonstrated in various lymphoid organs, the lung and abomasum of a goat ... | 1988 | 3140470 |
adaptation of the lapinized rinderpest virus to in vitro growth and attenuation of its virulence in rabbits. | a lapinized rinderpest virus, the l strain, which is virulent in rabbits and had been grown only in rabbits, was adapted to grow in vero cells by the fusion of vero cells with virus-infected rabbit spleen cells in the presence of polyethylene glycol, and subsequently passaged in vero cells by co-culture technique. after several passages, free virus was produced at high titre. the vero cell-adapted virus acquired the ability to infect several cell lines which were non-permissive to the unadapted ... | 1986 | 3944587 |
antibody response of cattle to rinderpest vaccine. | antibody production was studied in cattle infected with rinderpest vaccine virus. vaccinated cattle produced both igm and igg serum antibodies. the igg antibodies were mainly those of igg2 subclass. no iga antibody response was detected in vaccinated animals. | 1985 | 3984206 |
protection of cattle against rinderpest with vaccinia virus recombinants expressing the ha or f gene. | rinderpest is a highly contagious ruminant viral disease manifested by a rapid course and greater than 90% mortality. infectious vaccinia virus recombinants were constructed that express either the hemagglutinin or the fusion gene of rinderpest virus. all cattle vaccinated with either recombinant or with the combined recombinants produced neutralizing antibodies against rinderpest virus and were protected against the disease when challenged with more than 1000 times the lethal dose of the virus. | 1988 | 3194758 |
induction of antinuclear antibodies in rabbits by infection with rinderpest viruses with different degree of virulence. | | 1988 | 3264353 |
the ultrastructure of peste des petits ruminants virus. | | 1985 | 4050210 |
experimental peste des petits ruminants (goat plague) in goats and sheep. | in order to study the pathomorphology and immunohistochemistry of peste des petits ruminants, four goats and two sheep were inoculated intranasally with the malig-yemen strain of peste des petits ruminants virus. the animals developed fever, nasal discharge, oral erosions, cough and diarrhea. one goat and one sheep died and one moribund goat was killed. three animals survived the infection. at necropsy, erosive stomatitis, pneumonia and gastroenteritis were found. histopathologically the pneumon ... | 1988 | 3280108 |
further studies on the immunologic relationships among measles, distemper, and rinderpest viruses. | | 1974 | 4139195 |
fusion glycoprotein (f) of rinderpest virus: entire nucleotide sequence of the f mrna, and several features of the f protein. | the full-length cdna corresponding to the mrna for the fusion protein of rinderpest virus (rv) was cloned and its complete nucleotide sequence was determined. the mrna for the f protein was composed of 2359 nucleotides and contained a single large open reading frame which was capable of encoding 566 amino acids with a molecular weight (mw) of 58,929. the rv-f mrna had a long noncoding region at the 5' end (586 bases) which was c-rich like the measles virus (mv)-f mrna but they did not appear to ... | 1988 | 3285575 |
chemical structure of african swine fever virus investigated by electron microscopy. | | 1967 | 4168517 |
comparative immunofluorescent studies on measles, canine distemper, and rinderpest viruses. immunofluorescence of measles, distemper, and rinderpest viruses. | | 1970 | 4195094 |
ferritin-tagged antibody cross-reactions among rinderpest, canine distemper, and measles viruses. | | 1973 | 4201833 |
natural infection of cynomolgus monkeys with measles virus. | | 1973 | 4214280 |
[study of the immunostimulating activity of 3 imidazole derivatives with anthelminthic action, on bovipestic, peripneumonic and anthrax immunogenesis]. | | 1974 | 4216052 |
reverse phase passive haemagglutination test for the detection of rinderpest antigen. | reverse phase passive haemagglutination [rpha] test was applied for the detection of rinderpest antigen in various organs of rinderpest infected cattle. the results of rpha were compared with counter immunoelectrophoresis [cie] and single radial haemolysis [srh] test. rpha was as sensitive as cie and srh in detecting rinderpest antigen. | 1987 | 3299936 |
a reassessment of the dual vaccine against rinderpest and contagious bovine pleuropneumonia. | in the light of the recent outbreaks of rinderpest in africa a further assessment of the efficacy of the simultaneous inoculation of rinderpest virus vaccine and contagious bovine pleuropneumonia vaccine was undertaken. groups of cattle were inoculated with a dual preparation of rinderpest vaccine virus and mycoplasma mycoides subspecies mycoides or m mycoides alone. these groups were then challenged with m mycoides, first unsuccessfully by an in-contact challenge method and then by subcutaneous ... | 1987 | 3300010 |
nucleotide sequence of segment s10 of the rice dwarf virus genome. | dna complementary to the tenth largest (s10) of the 12 genome segments of rice dwarf virus (rdv) was cloned and its sequence was determined. this genome segment is 1319 nucleotides in length and has a single long open reading frame extending for 1059 nucleotides from the first aug triplet (residues 27 to 29). the predicted translation product comprises 352 amino acids and has a mol. wt. of 39094. rdv transcripts synthesized in vitro have the same polarity as the plus strand of the genome. termin ... | 1988 | 3335833 |
observations on cultured cells infected with rinderpest virus by means of fluorescent antibody technique. | | 1969 | 4309157 |
presence of 2 different viral agents in brain cells of patients with subacute sclerosing panencephalitis. | | 1970 | 4316388 |
the immunodepressive effect of rinderpest virus. | | 1970 | 4320166 |
clinical and serologic response of american white-collared peccaries to african swine fever, foot-and-mouth disease, vesicular stomatitis, vesicular exanthema of swine, hog cholera, and rinderpest viruses. | | 1969 | 4322827 |
cytopathic effect in cell cultures of rinderpest virus and some other viruses causing rinderpest-like diseases. | | 1970 | 4333486 |
factors influencing the epidemiology and epizootiology of airborne diseases. | | 1971 | 4342868 |
a diseases of sokoto goats resembling "peste des petits ruminants". | | 1972 | 4366855 |
observations on pathogenic organisms in the airborne state. | | 1972 | 4367142 |
analysis of the polypeptides synthesized in rinderpest virus-infected cells. | we have identified, by [35s]methionine labeling, eight major induced proteins and a number of minor proteins in rinderpest virus-infected bovine kidney cells. the polypeptides ranged in molecular weight from 212 to 21.5 kda. the majority of these polypeptides are virus specific, as demonstrated by immunoprecipitation with rabbit hyperimmune serum against rinderpest. infected cells radiolabeled with glucosamine contained a 75-kda polypeptide and a broad band migrating at 80 kda, both identified a ... | 1988 | 3354196 |
inactivation of rinderpest and ecbo viruses by hydroxylamine. | | 1967 | 4383037 |
active immunization of cattle with killed vaccines prepared from cell-cultured rinderpest virus. | | 1974 | 4425313 |
serum electrophoretic pattern of buffalo calves vaccinated with cell culture rinderpest vaccine. | | 1974 | 4464256 |
antigenic variation between three strains of rinderpest virus detected by kinetic neutralisation and competition elisa using early rabbit antisera. | strains of rinderpest virus (rv) with high and low virulence were compared with cell culture-attenuated rv for antigenic differences. sera collected from rabbits 1 week after inoculation with live virus distinguished between homologous and heterologous strains by kinetic neutralisation and competition elisa. | 1988 | 3376417 |
cloning of the fusion gene of rinderpest virus: comparative sequence analysis with other morbilliviruses. | we have cloned the cdna of the fusion (f) gene of the virulent (kabete o) strain of rinderpest virus and provided a comparative analysis of its sequence with that of the f genes of measles and distemper viruses. the gene has an open reading frame of 2241 nucleotides with two potential initiation codons in-frame. use of the first atg would produce a polypeptide 747 amino acids long with a calculated molecular weight of 81,068. however, we suggest that the second atg is used to generate the fo pro ... | 1988 | 3413983 |
cloning and sequence analysis of the hemagglutinin gene of the virulent strain of rinderpest virus. | we have cloned the cdna of the hemagglutinin (ha) gene of the virulent (kabete o) strain of rinderpest virus and produced a comparative analysis of its sequence with that of the ha genes of rinderpest (lapinized strain) and measles viruses. the gene has an open reading frame of 1827 nucleotides, and the derived protein is 609 amino acids long with a calculated molecular weight of 68,006. the kabete o ha polypeptide is identical in length to the ha of the lapinized strain of rinderpest virus and ... | 1988 | 3413986 |
[modern methods and technics in the experimental diagnosis of bovine plague]. | | 1973 | 4617907 |
[inopportune cattle mucosal diseases associated with rinderpest vaccine]. | | 1972 | 4677385 |
[small ruminant plague and its prevention in senegal and in western africa]. | | 1973 | 4807991 |
an epidemiological model of rinderpest. i. description of the model. | the development of an epidemiological model of rinderpest in cattle and wildlife populations is described. the model uses a state-transition structure, incorporating a stochastic element through the use of monte-carlo methods modified to allow large populations to be simulated. the potential applications include the estimation of "safe" host population immunity rates and the design of cost-effective rinderpest vaccination programmes. | 1989 | 2711464 |
relationships among measles, canine distemper and rinderpest viruses. | | 1968 | 4886887 |
[cattle-plague and immunofluorescence. application to the differential diagnosis of cattle-plague infection]. | | 1970 | 4934929 |
clinical and immune response of alien hosts to inoculation with measles, rinderpest, and canine distemper viruses. | | 1965 | 4956660 |
serologic response in monkeys inoculated with rinderpest and measles viruses. | | 1969 | 4980999 |
[some fundamental research on rinderpest virus. 2. rinderpest haemagglutinin. antigenic relationships of the rinderpest and measles viruses]. | | 1968 | 4984507 |
use of vero cells for titration of rinderpest virus and its neutralizing antibody. | primary bovine embryonic kidney cell was successfully replaced by the green monkey cell line (vero) for the isolation and seroneutralization of rinderpest virus. | 1970 | 4985833 |
[biological aspects of the multiplication of the plague virus of small ruminants or srp virus on cell cultures]. | | 1968 | 4985884 |
a comparative electron microscopic study on the morphogenesis of canine distemper and rinderpest viruses. | | 1971 | 4994230 |
subacute sclerosing panencephalitis, measles, rabies and progressive multifocal leukoencephalopathy. | | 1971 | 5000224 |
a comparative study of peste des petits ruminants and kata in west african dwarf goats. | | 1971 | 5006087 |
congenital infection of cattle with the herpesvirus causing malignant catarrhal fever. | | 1972 | 5017823 |
studies on rinderpest culture vaccine. iv. the stability of the reconstituted product. | | 1971 | 5103430 |
an epidemiological model of rinderpest. ii. simulations of the behaviour of rinderpest virus in populations. | fixed parameters for different hypothetical strains of rinderpest virus (rv) and different susceptible populations are described together with details of their derivation. simulations were then carried out in a computer model to determine the effects that varying these parameters would have on the behaviour of rv in the different populations. the results indicated that virulent strains of rv are more likely to behave in epidemic fashion whereas milder strains tend towards persistence and the est ... | 1989 | 2711465 |
immunization with a vaccinia recombinant expressing the f protein protects rabbits from challenge with a lethal dose of rinderpest virus. | a cdna clone containing the complete coding sequence of the rinderpest fusion protein (f) gene was inserted into the thymidine kinase gene of vaccinia virus (wr strain) under the control of the 7.5k early/late vaccinia virus promoter. all forms of the f protein, i.e., the glycosylated f0 precursor, the unglycosylated f1 protein, and the glycosylated f2 protein, were detected in cells infected with the recombinant virus. vaccination of rabbits with the recombinant virus induced antibodies which r ... | 1989 | 2718375 |
[comparative morphology of measles, distemper and rinderpest viruses]. | | 1971 | 5169406 |
an outbreak of peste des petits ruminants in a zoological collection. | peste des petits ruminants virus was suspected to be the cause of a disease outbreak in a zoological collection at al ain in the arabian gulf. clinically the outbreak affected gazelles (gazellinae), ibex and sheep (caprinae) and gemsbok (hippotraginae); subclinical involvement of nilgai (tragelaphinae) was suspected. a morbillivirus was isolated and using monoclonal antibodies and biological tests in cattle, sheep and goats the virus of peste des petits ruminants was identified. | 1987 | 3424615 |
investigations on the incidence of rinderpest virus infection in game animals of n. tanganyika and s. kenya 1960-63. | | 1967 | 5233989 |
[recently acquired knowledge on cattle plague and its virus]. | | 1966 | 5333398 |
single radial hemolysis test for the detection of rinderpest antibody. | the single radial hemolysis [srh] test was employed for detection of rinderpest antibodies in post-vaccinated serum samples as also in serum samples from animals recovered from rinderpest infection. the results were compared with counterimmunoelectrophoresis [cie] and serum neutralisation [sn] tests. the cie test was found to be more sensitive than srh but because of ease and simplicity srh can also be used for monitoring antibody development after vaccination. | 1986 | 3532467 |
[clinical and immunologic behavior during rinderpest contamination of cattle vaccinated several years ago against rinderpest with cell culture vaccines]. | | 1969 | 5408390 |
[adaptation in microtest of the seroneutralization technic by the kinetic method for the research and titration of antibodies neutralizing the rinderpest virus]. | | 1969 | 5408391 |
simple and rapid dot-enzyme immunoassay for visual detection of rinderpest antibodies in bovine and caprine sera. | a modified solid phase enzyme immunoassay (eia) is described for visual detection of anti-rinderpest virus (rpv) antibodies in cattle and goat sera. dots of rpv antigens were adsorbed to nitrocellulose (nc) paper (hence dot-eia) and the adsorptive reactive sites were blocked with skim milk powder. after immersion in bovine or caprine test serum bound antibodies were reacted with a peroxidase-conjugated anti-bovine or anti-caprine igg (h & l), respectively. positive reactions were easily visualiz ... | 1986 | 3544400 |
comparison of proteins induced in cells infected with rinderpest and peste des petits ruminants viruses. | the two morbilliviruses rinderpest virus (rpv) and peste des petits ruminants virus (pprv) are closely related and cause severe disease in large and small ruminants, respectively. they show distinct epidemiological patterns and are distinguishable by reciprocal cross-neutralization tests. we have analysed the proteins induced by these viruses in infected cells and have shown that they can be distinguishable by a very marked difference in the apparent mol. wt. of the nucleocapsid (n) protein. the ... | 1987 | 3598564 |
a new method of evaluation of the potency of anti rinderpest serum. | | 1970 | 5439960 |
molecular cloning and sequence analysis of the rinderpest virus mrna encoding the hemagglutinin protein. | we cloned the full-length cdnas corresponding to the mrna for the hemagglutinin (h) protein of rinderpest virus (rv) and determined the nucleotide sequence of rv-h. the gene of rv-h was composed of 1952 nucleotides and contained a single large open reading frame, which was capable of encoding a protein of 609 amino acids with a molecular weight of 68,330 da. the nucleotide sequence and predicted amino acid sequence were compared with those of the measles virus (mv)-h. the 5' end of the message ( ... | 1987 | 3629979 |
a plaque assay system for rinderpest virus and its use in characterising virus adsorption. | | 1970 | 5533699 |
studies on rinderpest culture vaccine. 3. stability of the lyophilised product. | | 1970 | 5533977 |
a recent outbreak of rinderpest in east africa. | rinderpest was brought under control in kenya in 1976 but in april 1986 an outbreak of the disease occurred in cattle in western kenya, five kilometres from the kenya-uganda border. this was the first confirmed field outbreak of the disease in kenya after a lull of over 10 years. clinical disease was confined to unvaccinated zebu calves aged six to eight months from which rinderpest virus was isolated. high titres of antibodies to rinderpest virus were demonstrated in sera collected from sheep a ... | 1987 | 3660452 |
the pathogenesis of rinderpest in the lymph nodes of cattle. light and electron microscopic studies. | | 1971 | 5540659 |
studies on lapinised rinderpest virus. | | 1971 | 5566706 |
application of solid phase aggregation of coated erythrocytes technique for detection of rinderpest antigen. | a solid phase aggregation of coated erythrocytes (space) technique was standardized and used for the detection of rinderpest antigen in 129 samples of 9 visceral organs; from 50 clinical samples such as gum scraping, tongue scraping, faeces, urine, nasal swabs; blood from 18 rinderpest infected cattle and 2 field samples. the comparative sensitivity of space, reverse phase passive haemagglutination and counter-immuno-electrophoresis tests was found to be 86.8, 84.5 and 79.8%, respectively. | 1987 | 3667851 |
continuing presence of rinderpest virus as a threat in east africa, 1983-1985. | the re-emergence of rinderpest virus in east africa in 1979 caused widespread outbreaks of disease and subclinical infection throughout the region until mid-1983. subsequent massive emergency vaccination campaigns have been successful in eliminating clinical rinderpest from tanzania and preventing its spread southwards. unfortunately the virus is still endemic in north-eastern uganda and has recently caused epidemic outbreaks with high mortality in cattle in that country. in kenya, buffaloes (sy ... | 1987 | 3824842 |
notes on immunoprecipitin reactions with rinderpest virus. | | 1985 | 3922099 |
simple techniques for increasing the batch size of rinderpest cell culture vaccine. | | 1985 | 3992673 |
experimental infection of sheep and goats with attenuated and virulent strains of rinderpest virus. | | 1967 | 5625712 |
comparison of messenger rnas induced in cells infected with each member of the morbillivirus group. | virus-specific mrnas radiolabelled with [32p]orthophosphate in the presence of actinomycin d were extracted from the cytoplasm of vero cells infected with each of the known morbilliviruses: measles virus, canine distemper virus, rinderpest virus, and peste des petits ruminants virus. when analysed on denaturing agarose-formaldehyde gels the major rna species from all viruses in the group were identical, except for canine distemper virus where one of the virus-specific mrnas (mrna 5), which proba ... | 1985 | 4013082 |