| diagnostic accuracy of ns1 elisa and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responses. | dengue is a public health problem in many countries. rapid diagnosis of dengue can assist patient triage and management. detection of the dengue viral protein, ns1, represents a new approach to dengue diagnosis. | 2009 | 19156192 |
| dengue hemorrhagic fever caused by sequential dengue 1-3 virus infections over a long time interval: havana epidemic, 2001-2002. | a dengue epidemic caused by dengue virus 3 (denv-3) occurred in cuba in 2001-2002. it included cases of dengue hemorrhagic fever/dengue shock syndrome (dhf/dss). we report neutralizing antibody studies on sera from 54 of 78 dhf/dss patients that provide evidence of infections occurring in the sequence denv-1 followed by denv-3. no sera showed infection in the sequence denv-2 followed by denv-3. some sera showed a pattern of infection in the sequence denv-1 followed by denv-2 and then denv-3. how ... | 2006 | 17172378 |
| dengue-3 virus genomic differences that correlate with in vitro phenotype on a human cell line but not with disease severity. | we compared the full genome sequence of nine clinical isolates of dengue virus obtained during an epidemic of dengue-3 in french polynesia in 1989, from patients with various presentations of disease. the isolates, all belonging to genotype i, had 25 amino acid substitutions. there was no association with disease severity. when cultured in the k562 human erythroleukemia cell line, the isolates induced a range of cell growth inhibitions that was not associated with the degree of disease severity. ... | 2007 | 17196421 |
| evaluation of a commercial real-time pcr kit for detection of dengue virus in samples collected during an outbreak in goiania, central brazil, in 2005. | in the past 2 decades, dengue has reemerged in brazil as a significant public health problem. clinicians demand a diagnostic test with high sensitivity that is applicable during the early symptomatic phase. we aimed to test two distinct molecular methods on samples from suspected dengue cases during an outbreak in central brazil. acute-phase serum specimens from 254 patients suspected of having dengue were collected during 2005 in the city of goiânia, central brazil. samples were blindly evaluat ... | 2007 | 17409201 |
| high levels of subgenomic hcv plasma rna in immunosilent infections. | a genetic analysis of hepatitis c virus (hcv) in rare blood donors who remained hcv seronegative despite long-term high-level viremia revealed the chronic presence of hcv genomes with large in frame deletions in their structural genes. full-length hcv genomes were only detected as minority variants. in one immunodeficiency virus (hiv) co-infected donor the truncated hcv genome transiently decreased in frequency concomitant with delayed seroconversion and re-emerged following partial seroreversio ... | 2007 | 17493654 |
| dengue viruses activity in piauí, brazil. | the present paper reports a laboratory investigation performed between the years of 2000 and 2002 to study a virological surveillance program introduced in the state of piauí to support an epidemiological survey of the disease. dengue virus type 3 (denv-3) existence in the state was detected in may 2002 when a high number of dengue cases due to denv-1 and denv-2 were reported. an assessment on the population knowledge about the disease and its transmission showed that almost 50% of the populatio ... | 2003 | 15049083 |
| peptide bbeta(15-42) preserves endothelial barrier function in shock. | loss of vascular barrier function causes leak of fluid and proteins into tissues, extensive leak leads to shock and death. barriers are largely formed by endothelial cell-cell contacts built up by ve-cadherin and are under the control of rhogtpases. here we show that a natural plasmin digest product of fibrin, peptide bbeta15-42 (also called fx06), significantly reduces vascular leak and mortality in animal models for dengue shock syndrome. the ability of bbeta15-42 to preserve endothelial barri ... | 2009 | 19401765 |
| specific genetic markers for detecting subtypes of dengue virus serotype-2 in isolates from the states of oaxaca and veracruz, mexico. | dengue (den) is an infectious disease caused by the den virus (denv), which belongs to the flavivirus genus in the family flaviviridae. it has a (+) sense rna genome and is mainly transmitted to humans by the vector mosquito aedes aegypti. dengue fever (df) and dengue hemorrhagic fever (dhf) are caused by one of four closely related virus serotypes (denv-1, denv-2, denv-3 and denv-4). epidemiological and evolutionary studies have indicated that host and viral factors are involved in determining ... | 2008 | 18625078 |
| an imported dengue fever case by dengue virus 3 (denv-3) infection in gunma, japan. | | 2008 | 18219146 |
| elevated levels of soluble st2 protein in dengue virus infected patients. | levels of the soluble form of the interleukin-1 receptor-like 1 protein (il-1rl-1/st2) are elevated in the serum of patients with diseases characterized by an inflammatory response. the objective of this study was to determine the concentration of soluble st2 (sst2) in dengue infected patients during the course of the disease. twenty-four patients with confirmed dengue infection, classified as dengue fever, and 11 patients with other febrile illness (ofi) were evaluated. levels of sst2 in serum ... | 2008 | 18226917 |
| molecular epidemiology of dengue virus type 3 in northern south america: 2000--2005. | a phylogenetic approach was used to identify genetic variants of denv-3 subtype iii that may have emerged during or after its expansion throughout south america. we sequenced the capsid, premembrane/membrane and envelope genes from 22 denv-3 strains isolated from venezuela, bolivia, ecuador and peru between 2000 and 2005. phylogenetic analysis showed that the isolates sequenced in this study formed three clades within subtype iii: one with the isolates from venezuela, one with the bolivian isola ... | 2008 | 18674640 |
| simultaneous circulation of genotypes i and iii of dengue virus 3 in colombia. | dengue is a major health problem in tropical and subtropical regions. in colombia, dengue viruses (denv) cause about 50,000 cases annually, 10% of which involve dengue haemorrhagic fever/dengue shock syndrome. the picture is similar in other surrounding countries in the americas, with recent outbreaks of severe disease, mostly associated with denv serotype 3, strains of the indian genotype, introduced into the americas in 1994. | 2008 | 18764951 |
| evidence for the co-circulation of dengue virus type 3 genotypes iii and v in the northern region of brazil during the 2002-2004 epidemics. | the reintroduction of dengue virus type 3 (denv-3) in brazil in 2000 and its subsequent spread throughout the country was associated with genotype iii viruses, the only denv-3 genotype isolated in brazil prior to 2002. we report here the co-circulation of two different denv-3 genotypes in patients living in the northern region of brazil during the 2002-2004 epidemics. complete genomic sequences of viral rna were determined from these epidemics, and viruses belonging to genotypes v (southeast asi ... | 2008 | 18797763 |
| spatial and temporal clustering of dengue virus transmission in thai villages. | transmission of dengue viruses (denv), the leading cause of arboviral disease worldwide, is known to vary through time and space, likely owing to a combination of factors related to the human host, virus, mosquito vector, and environment. an improved understanding of variation in transmission patterns is fundamental to conducting surveillance and implementing disease prevention strategies. to test the hypothesis that denv transmission is spatially and temporally focal, we compared geographic and ... | 2008 | 18986209 |
| genetic diversity of the e protein of dengue type 3 virus. | dengue is the most important arbovirus disease in tropical and subtropical countries. the viral envelope (e) protein is responsible for cell receptor binding and is the main target of neutralizing antibodies. the aim of this study was to analyze the diversity of the e protein gene of denv-3. e protein gene sequences of 20 new viruses isolated in ribeirao preto, brazil, and 427 sequences retrieved from genbank were aligned for diversity and phylogenetic analysis. | 2009 | 19627608 |
| a dna vaccine candidate expressing dengue-3 virus prm and e proteins elicits neutralizing antibodies and protects mice against lethal challenge. | in an effort to develop a suitable dna vaccine candidate for dengue, using dengue-3 virus (denv-3) as a prototype, the genes coding for premembrane (prm) and envelope proteins (e) were inserted into an expression plasmid. after selecting recombinant clones containing prm/e genes, protein expression in the cell monolayer was detected by indirect immunofluorescence and immunoprecipitation assays. after selecting three vaccine candidates (pvac1den3, pvac2den3 and pvac3den3), they were analyzed in v ... | 2008 | 19002647 |
| phylogeography and evolutionary history of dengue virus type 3. | in this study, we revisited the phylogeography of the three of major denv-3 genotypes and estimated its rate of evolution, based on the analysis of the envelope (e) gene of 200 strains isolated from 31 different countries around the world over a time period of 50 years (1956-2006). our phylogenetic analysis revealed a geographical subdivision of denv-3 population in several country-specific clades. migration patterns of the main denv-3 genotypes showed that genotype i was mainly circumspect to t ... | 2009 | 19010450 |
| mode of transmission and the evolution of arbovirus virulence in mosquito vectors. | the traditional assumption that vector-borne pathogens should evolve towards a benign relationship with their arthropod vectors has been challenged on theoretical grounds and empirical evidence. however, in the case of arboviruses (arthropod-borne viruses), although a number of investigators have reported experimental evidence for virus-induced vector mortality, others have failed to detect any significant impact. whether this variation in the observed level of arbovirus virulence depends on bio ... | 2009 | 19141420 |
| development of real time pcr for detection and quantitation of dengue viruses. | dengue virus (denv), a mosquito borne flavivirus is an important pathogen causing more than 50 million infections every year around the world. dengue diagnosis depends on serology, which is not useful in the early phase of the disease and virus isolation, which is laborious and time consuming. there is need for a rapid, sensitive and high throughput method for detection of denv in the early stages of the disease. several real-time pcr assays have been described for dengue viruses, but there is s ... | 2009 | 19166574 |
| quantification of dengue virus type 3 rna in fatal and non-fatal cases in brazil, 2002. | we examined levels of dengue virus type 3 (denv-3) rna in association with the type of infection (primary or secondary) in 42 patients with fatal and non-fatal outcomes in rio de janeiro, brazil, 2002. subjects with fatal outcomes had mean virus titers significantly higher than those who survived (12.5 vs. 7.9 log(10) rna copies/ml). because primary infections were confirmed among the fatal cases (52.1%), antibody-dependent enhancement alone did not explain all the cases of severe disease in thi ... | 2009 | 19261315 |
| a role for autophagolysosomes in dengue virus 3 production in hepg2 cells. | we have recently proposed that amphisomes act as a site for translation and replication of dengue virus (denv)-2 and that denv-2 entry and replication are linked through an ongoing association with membranes of an endosomal-autophagosomal lineage. in this report, we present the results of an investigation into the interaction between denv-3 and the autophagy machinery. critically, treatment with the lysosomal fusion inhibitor l-asparagine differentiated the interaction of denv-3 from that of den ... | 2009 | 19264601 |
| dengue virus type 3 infection in traveler returning from west africa. | | 2009 | 19891895 |
| pulmonary hemorrhage syndrome associated with dengue fever, high-resolution computed tomography findings: a case report. | dengue hemorrhagic fever is an acute infectious disease caused by dengue virus. we described the high-resolution ct findings in a 70-year-old male with the disease, which was diagnosed by clinical examination and confirmed by serological methods. high-resolution ct demonstrated bilateral areas of consolidation with air bronchogram and ground glass opacities, as well as small bilateral pleural effusions. dengue hemorrhagic fever should be considered in the differential diagnosis of diffuse pulmon ... | 2009 | 19265524 |
| evidence of diversification of dengue virus type 3 genotype iii in the south american region. | in order to gain insight into the genetic variability of dengue virus type 3 (denv-3) genotype iii isolated in the latin american region, phylogenetic analysis were carried out using envelope (e) gene sequences from 57 denv-3 genotype iii strains isolated in 11 latin american countries. at least six different genotype iii clades were observed. amino acids substitutions were found in domain iii e protein neutralization epitopes and in surface-exposed domain ii and iii e protein amino acid sequenc ... | 2009 | 19322636 |
| molecular epidemiology of dengue virus strains from finnish travelers. | we characterized 11 dengue virus (denv) isolates obtained from finnish travelers during 2000-2005 using monoclonal antibodies and phylogenetic analysis. the analysis of denv isolated from travelers contributes to the global picture of strain distribution and circulation. the isolates included all serotypes, including a denv-2 isolate from ghana. | 2008 | 18258084 |
| dengue virus 3 genotype 1 associated with dengue fever and dengue hemorrhagic fever, brazil. | dengue serotype 3 viruses were isolated from patients in brazil from 2002 through 2004. on the basis of phylogenetic analyses, these isolates were assigned genotype 1. this genotype had never been reported in south america before. its appearance indicates a major risk factor for dengue epidemics and severe disease. | 2008 | 18258129 |
| asymmetric competitive suppression between strains of dengue virus. | within-host competition between strains of a vector-borne pathogen can affect strain frequencies in both the host and vector, thereby affecting viral population dynamics. however little is known about inter-strain competition in one of the most genetically diverse and epidemiologically important mosquito-borne rna virus: dengue virus (denv). to assess the strength and symmetry of intra-host competition among different strains of denv, the effect of mixed infection of two denv serotypes, denv2 an ... | 2008 | 18261207 |
| superior infectivity for mosquito vectors contributes to competitive displacement among strains of dengue virus. | competitive displacement of a weakly virulent pathogen strain by a more virulent strain is one route to disease emergence. however the mechanisms by which pathogens compete for access to hosts are poorly understood. among vector-borne pathogens, variation in the ability to infect vectors may effect displacement. the current study focused on competitive displacement in dengue virus serotype 3 (denv3), a mosquito-borne pathogen of humans. in sri lanka in the 1980's, a native denv3 strain associate ... | 2008 | 18269771 |
| dengue virus 3 clinical isolates show different patterns of virulence in experimental mice infection. | dengue virus (denv) may cause symptomatic infection with mild, undifferentiated febrile illness called classical dengue fever (df) or a more severe disease, potentially fatal, known as dengue hemorrhagic fever (dhf) or dengue shock syndrome. the pathogenesis of dhf is based on the virulence of the infecting denv and depends on the infecting serotypes and genotypes; it is also based on the immunopathogenesis that is mediated by host immune responses, including dengue virus-cross-reactive antibodi ... | 2010 | 20381635 |
| chikungunya fever in travelers returning to europe from the indian ocean region, 2006. | chikungunya fever has spread through several indian ocean islands and india, including popular travel destinations. to compare usefulness of diagnostic tests and to understand reasons for the magnitude and severity of an outbreak, we used 3 diagnostic methods to test 720 samples from 680 patients returning to europe from the indian ocean region in 2006. chikungunya infection was confirmed for 24.4% patients in the first half of the year and for 9.9% in the second half. reverse transcription-pcr ... | 2008 | 18325256 |
| continued persistence of a single genotype of dengue virus type-3 (denv-3) in delhi, india since its re-emergence over the last decade. | the re-emergence of an epidemic strain of dengue virus type-3 (denv-3) in delhi in 2003 and its persistence in subsequent years marked a changing trend in dengue virus circulation in this part of india. its evolving phylogeny over the past decade has not been studied in detail as yet. | 2010 | 20434124 |
| genetic analysis of dengue virus type 3 isolated in buenos aires, argentina. | dengue virus as a member of mosquito-borne flaviviruses is responsible for an increasing number of human infections worldwide, mainly in tropical and subtropical urban areas. the agent, a single-stranded positive sense rna virus, is comprised of four serotypes and genetic variation within each serotype can be further divided into different genotypes. dengue outbreaks were reported in bordering countries during the last years; the latest reported in paraguay in 2006-2007. in buenos aires, 32 deng ... | 2008 | 18400327 |
| high-throughput procedure for tick surveys of tick-borne encephalitis virus and its application in a national surveillance study in switzerland. | tick-borne encephalitis (tbe), a viral infection of the central nervous system, is endemic in many eurasian countries. in switzerland, tbe risk areas have been characterized by geographic mapping of clinical cases. since mass vaccination should significantly decrease the number of tbe cases, alternative methods for exposure risk assessment are required. we established a new pcr-based test for the detection of tbe virus (tbev) in ticks. the protocol involves an automated, high-throughput nucleic ... | 2010 | 20453126 |
| genetic analysis of dengue 3 virus subtype iii 5' and 3' non-coding regions. | the emergence of dengue hemorrhagic fever in sri lanka in 1989 correlated with the appearance of a genetic variant of dengue 3 virus (denv-3) subtype iii (group b), closely related to the endemic group a variant. we studied the 5' and 3' non-coding regions (ncrs) of 15 denv-3 subtype iii isolates from sri lanka, nicaragua and martinique and found variability in the 3' ncrs. this included an 11-nucleotide insertion common to all isolates examined and two nucleotide differences that segregated vir ... | 2008 | 18436323 |
| comparative analysis of full genomic sequences among different genotypes of dengue virus type 3. | although the previous study demonstrated the envelope protein of dengue viruses is under purifying selection pressure, little is known about the genetic differences of full-length viral genomes of denv-3. in our study, complete genomic sequencing of denv-3 strains collected from different geographical locations and isolation years were determined and the sequence diversity as well as selection pressure sites in the denv genome other than within the e gene were also analyzed. | 2008 | 18495043 |
| multiplex cytokine profile from dengue patients: mip-1beta and ifn-gamma as predictive factors for severity. | dengue virus pathogenesis is not yet fully understood and the identification of patients at high risk for developing severe disease forms is still a great challenge in dengue patient care. during the present study, we evaluated prospectively the potential of cytokines present in plasma from patients with dengue in stratifying disease severity. | 2008 | 18578883 |
| prolonged co-circulation of two distinct dengue virus type 3 lineages in the hyperendemic area of medellin, colombia. | during the past two decades, dengue virus-3 (denv-3) has re-emerged in the western hemisphere causing significant epidemics of dengue fever (df) and dengue hemorrhagic fever (dhf). in an effort to understand the molecular evolution of denv-3 and their relationships to other denv-3 circulating in the western hemisphere, we conducted a phylogenetic study on denv-3 isolates made between 2002 and 2007 in the metropolitan area of medellín, colombia. an unexpected co-circulation of two different varia ... | 2010 | 20810837 |
| displacement of dengue virus type 3 and type 2 by dengue virus type 1 in delhi during 2008. | | 2010 | 20966588 |
| profile of hemophagocytic lymphohistiocytosis in children in a tertiary care hospital in india. | to describe the epidemiology, clinical features, laboratory findings, outcome and the difficulties in diagnosis and management of children with hemo-phagocytic lymphohistiocytosis (hlh) in a tertiary children's hospital in india. | 2010 | 20972297 |
| spatio-temporal tracking and phylodynamics of an urban dengue 3 outbreak in são paulo, brazil. | the dengue virus has a single-stranded positive-sense rna genome of approximately 10.700 nucleotides with a single open reading frame that encodes three structural (c, prm, and e) and seven nonstructural (ns1, ns2a, ns2b, ns3, ns4a, ns4b, and ns5) proteins. it possesses four antigenically distinct serotypes (denv 1-4). many phylogenetic studies address particularities of the different serotypes using convenience samples that are not conducive to a spatio-temporal analysis in a single urban setti ... | 2009 | 19478848 |
| importation of dengue virus type 3 to japan from tanzania and cote d'ivoire. | travelers can introduce viruses from disease-endemic to non-disease-endemic areas. serologic and virologic tests confirmed dengue virus infections in 3 travelers returning to japan: 2 from tanzania and 1 from cote d'ivoire. phylogenetic analysis of the envelope gene showed that 2 genetically related virus isolates belonged to dengue virus type 3 genotype iii. | 2010 | 21029541 |
| evolution of dengue virus type 3 genotype iii in venezuela: diversification, rates and population dynamics. | dengue virus (denv) is a member of the genus flavivirus of the family flaviviridae. denv are comprised of four distinct serotypes (denv-1 through denv-4) and each serotype can be divided in different genotypes. currently, there is a dramatic emergence of denv-3 genotype iii in latin america. nevertheless, we still have an incomplete understanding of the evolutionary forces underlying the evolution of this genotype in this region of the world. in order to gain insight into the degree of genetic v ... | 2010 | 21087501 |
| different innate signatures induced in human monocyte-derived dendritic cells by wild-type dengue 3 virus, attenuated but reactogenic dengue 3 vaccine virus, or attenuated nonreactogenic dengue 1-4 vaccine virus strains. | dna microarrays were used to assess the innate gene signature in human myeloid dendritic cells infected with chimeric dengue 1-4 vaccines, a wild-type dengue 3 virus, or a classically attenuated serotype 3 vaccine shown to be reactogenic in humans. we observed a very reproducible signature for each of the 4 chimeric dengue vaccines, involving stimulation of type i interferon and associated genes, together with genes encoding chemokines and other mediators involved in the initiation of adaptive r ... | 2011 | 21148502 |
| [diagnosis of a dengue fever outbreak in yiwu city, zhejiang province in 2009 and its molecular tracing of the pathogen]. | to determine a dengue fever outbreak in yiwu city, zhejiang province in 2009 and to trace the origin of the pathogen. | 2010 | 21215110 |
| meningitis determined by oligosymptomatic dengue virus type 3 infection: report of a case. | dengue infection is a mosquito-borne disease caused by a flavivirus, and is recognized in over 100 countries with 2.5 billion people living in areas of risk. neurological manifestations such as encephalitis, myelitis, guillain-barré syndrome, cranial nerve palsies, neuromyelitis optica, and encephalomyelitis have been recognized as clinical consequences of dengue infection. meningitis is a rare complication. we report the case of a 24-year-old woman who presented with fever, headache, and nuchal ... | 2010 | 19501535 |
| dengue virus type 3 in brazil: a phylogenetic perspective. | circulation of a new dengue virus (denv)-3 genotype was recently described in brazil and colombia, but the precise classification of this genotype has been controversial. here we perform phylogenetic and nucleotide-distance analyses of the envelope gene, which support the subdivision of denv-3 strains into five distinct genotypes (gi to gv) and confirm the classification of the new south american genotype as gv. the extremely low genetic distances between brazilian gv strains and the prototype p ... | 2009 | 19547883 |
| comparative complete genome analysis of dengue virus type 3 circulating in india between 2003 and 2008. | dengue is endemic in most parts of the tropics including india. so far, complete genome information for indian dengue isolates is not available. in the present study, we characterized the genome of three dengue type 3 viruses isolated from india. the genomes of all three viruses were found to be 10,707 bp long with an orf encoding 3390 aa. extensive molecular phylogenetic analysis based on comparison of the complete genome and envelope gene classified the recent indian viruses into genotype iii ... | 2011 | 21411675 |
| molecular genetic relationship of the 3' untranslated region among thai dengue-3 virus, bangkok isolates, during 1973-2000. | dengue virus serotype 3 (denv-3) was associated with severe dengue epidemics in thailand during 1973-1999. we studied thai denv-3 viruses isolated from hospitalized children in bangkok with differing disease severity during that period. viruses were sequenced at their 5' and 3' untranslated regions (utrs), which are regions that play a pivotal role in viral replication. our results indicated that the primary sequences as well as the secondary structures at both ends of thai denv-3 viruses were h ... | 2009 | 19563250 |
| phylogeny of dengue virus type 3 circulating in colombia between 2001 and 2007. | dengue virus type 3 (denv-3) re-appeared in colombia in 2001 after 23 years of apparent absence, in the state of santander in the north-eastern region near to venezuelan border. in 2002, the virus was isolated in the state of valle del cauca in the south-east region near to ecuadorian/peruvian border, and in the state of antioquia in the north-east region near to panama border. to gain insight into the molecular epidemiology of denv-3 in colombia, we sequenced the complete e gene of 21 isolates ... | 2009 | 19619216 |
| co-infections with chikungunya virus and dengue virus in delhi, india. | aedes aegypti mosquitoes are common vectors for dengue virus and chikungunya virus. in areas where both viruses cocirculate, they can be transmitted together. during a dengue outbreak in delhi in 2006, 17 of 69 serum samples were positive for chikungunya virus by reverse transcription-pcr; 6 samples were positive for both viruses. | 2009 | 19624923 |
| induction of neutralization antibodies in mice by dengue-2 envelope dna vaccines. | dengue (den) viruses have become a public health problem that affects approximately 100 million people worldwide each year. prevention measures rely on vector control programs, which are inefficient. therefore, a vaccine is urgently needed. | 2009 | 19715116 |
| dual infection with dengue virus 3 and human immunodeficiency virus 1 in havana, cuba. | although dengue virus (den) endemic regions overlap with human immunodeficiency virus 1 (hiv) high incidence areas, little has been documented on hiv and den mixed infection. here we report den/hiv concurrent infections recorded during the den-3 epidemic in 2001-2002 in havana. serologic-confirmed den is described in two hiv-infected subjects with dengue fever symptoms. although patients had dengue disease, the cd4+ cells remained within normal levels and no accelerated progression of hiv diseas ... | 2009 | 19759497 |
| construction and characterization of a stable subgenomic replicon system of a brazilian dengue virus type 3 strain (br den3 290-02). | dengue viruses (denv) cause the most common arboviral disease afflicting men. clinical manifestations range from asymptomatic to dengue hemorrhagic fever/dengue shock syndrome (dhf/dss). the mechanisms involved in the disease pathogenesis are not fully understood. the severity of the disease seems to be influenced by both viral and host factors. subgenomic replicons of denv can be used to study viral replication mechanisms and evaluate the effects of antiviral drugs on viral replication. the obj ... | 2010 | 19761796 |
| new genotype of dengue type 3 virus circulating in brazil and colombia showed a close relationship to old asian viruses. | dengue type 3 genotype v viruses have been recently detected in brazil and colombia. in this study, we described another brazilian isolate belonging to this genotype. phylogenetic analysis including dengue type 3 viruses isolated worldwide showed that brazilian and colombian viruses were closely related to viruses isolated in asia more than two decades ago. the characteristic evolutionary pattern of dengue type 3 virus cannot explain the close similarity of new circulating viruses with old virus ... | 2009 | 19823677 |
| identification of continuous human b-cell epitopes in the envelope glycoprotein of dengue virus type 3 (denv-3). | dengue virus infection is a growing global public health concern in tropical and subtropical regions of the world. dengue vaccine development has been hampered by concerns that cross-reactive immunological memory elicited by a candidate vaccine could increase the risk of development of more severe clinical forms. one possible strategy to reduce risks associated with a dengue vaccine is the development of a vaccine composed of selected critical epitopes of each of the serotypes. | 2009 | 19826631 |
| identification of dengue-specific b-cell epitopes by phage-display random peptide library. | dengue is the most important human viral disease transmitted by arthropod vectors. the availability of random peptide libraries (rpl) displayed on phage has provided a powerful tool for selecting sequences that mimic epitopes from microorganisms that are useful for diagnostic and vaccine development purposes. in this paper, we describe peptides that resemble the antigenic structure of b-cell epitopes of dengue virus identified from a phage-peptide library using human sera containing polyclonal a ... | 2009 | 22135507 |
| phase 1 trial of the dengue virus type 4 vaccine candidate rden4{delta}30-4995 in healthy adult volunteers. | rden4delta30-4995 is a live attenuated dengue virus type 4 (denv4) vaccine candidate specifically designed as a further attenuated derivative of the rden4delta30 parent virus. in a previous study, 5 of 20 vaccinees who received 10(5) plaque-forming units (pfu) of rden4delta30 developed a transient elevation of the serum alanine aminotransferase (alt) level and an asymptomatic maculopapular rash developed in 10 of 20. in the current study, 28 healthy adult volunteers were randomized to receive 10 ... | 2009 | 19861619 |
| pcp consensus sequences of flaviviruses: correlating variance with vector competence and disease phenotype. | computational methods are needed to design multivalent vaccines against flaviviruses (fvs) such as the west nile virus or the dengue virus (denv). | 2010 | 19969003 |
| predicting the sensitivity and specificity of published real-time pcr assays. | in recent years real-time pcr has become a leading technique for nucleic acid detection and quantification. these assays have the potential to greatly enhance efficiency in the clinical laboratory. choice of primer and probe sequences is critical for accurate diagnosis in the clinic, yet current primer/probe signature design strategies are limited, and signature evaluation methods are lacking. | 2008 | 18817537 |
| development of a recombinant tetravalent dengue virus vaccine: immunogenicity and efficacy studies in mice and monkeys. | truncated recombinant dengue virus envelope protein subunits (80e) are efficiently expressed using the drosophila schneider-2 (s2) cell expression system. binding of conformationally sensitive antibodies as well as x-ray crystal structural studies indicate that the recombinant 80e subunits are properly folded native-like proteins. combining the 80e subunits from each of the four dengue serotypes with iscomatrix adjuvant, an adjuvant selected from a set of adjuvants tested for maximal and long la ... | 2010 | 20097152 |
| intrinsic antibody-dependent enhancement of microbial infection in macrophages: disease regulation by immune complexes. | a wide range of microorganisms can replicate in macrophages, and cell entry of these pathogens via non-neutralising igg antibody complexes can result in increased intracellular infection through idiosyncratic fcγ-receptor signalling. the activation of fcγ receptors usually leads to phagocytosis. paradoxically, the ligation of monocyte or macrophage fcγ receptors by igg immune complexes, rather than aiding host defences, can suppress innate immunity, increase production of interleukin 10, and bia ... | 2010 | 20883967 |
| differential regulation of toll-like receptor-2, toll-like receptor-4, cd16 and human leucocyte antigen-dr on peripheral blood monocytes during mild and severe dengue fever. | dengue fever (df), a public health problem in tropical countries, may present severe clinical manifestations as result of increased vascular permeability and coagulation disorders. dengue virus (denv), detected in peripheral monocytes during acute disease and in in vitro infection, leads to cytokine production, indicating that virus-target cell interactions are relevant to pathogenesis. here we investigated the in vitro and in vivo activation of human peripheral monocytes after denv infection. t ... | 2010 | 20113369 |
| multi-gene detection and identification of mosquito-borne rna viruses using an oligonucleotide microarray. | arthropod-borne viruses are important emerging pathogens world-wide. viruses transmitted by mosquitoes, such as dengue, yellow fever, and japanese encephalitis viruses, infect hundreds of millions of people and animals each year. global surveillance of these viruses in mosquito vectors using molecular based assays is critical for prevention and control of the associated diseases. here, we report an oligonucleotide dna microarray design, termed arbochip5.1, for multi-gene detection and identifica ... | 2013 | 23967358 |
| recent expansion of dengue virus serotype 3 in west africa. | due to non-existing or limited surveillance in africa, little is known about the epidemiology of dengue illness in the continent. serological and virological data obtained from returning european travellers is a key complement to this often flawed information. in the past years, dengue 3 virus has emerged in west africa and has been detected in travellers returning to europe. the first dengue epidemic in cape verde with more than 17,000 cases from september to december 2009 demonstrated that den ... | 2010 | 20184854 |
| dengue hemorrhagic fever: the sensitivity and specificity of the world health organization definition for identification of severe cases of dengue in thailand, 1994-2005. | dengue virus infection causes a spectrum of clinical manifestations, usually classified according to the world health organization (who) guidelines into dengue fever (df) and dengue hemorrhagic fever (dhf). the ability of these guidelines to categorize severe dengue illness has recently been questioned. | 2010 | 20205587 |
| [hematological abnormalities in patients with dengue]. | dengue is a neglected disease with high morbidity and mortality among children and adults that occurs mainly in tropical and subtropical regions. the objective of this study was to evaluate hematological changes in patients with clinical manifestations of dengue. medical records relating to 543 cases of dengue virus 3 that occurred during the 2007 epidemic in campo grande, mato grosso do sul, were studied. cases of classic dengue predominated (90.2%), with mild clinical manifestations lacking co ... | 2009 | 20209355 |
| memory cd8+ t cells from naturally acquired primary dengue virus infection are highly cross-reactive. | cross-reactive memory t cells induced by primary infection with one of the four serotypes of dengue virus (denv) are hypothesized to have an immunopathological function in secondary heterologous denv infection. to define the t-cell response to heterologous serotypes, we isolated hla-a(*)1101-restricted epitope-specific cd8(+) t-cell lines from primary denv-immune donors. cell lines exhibited marked cross-reactivity toward peptide variants representing the four denv serotypes in tetramer binding ... | 2011 | 20421879 |
| characterization of a dengue type-specific epitope on dengue 3 virus envelope protein domain iii. | dengue virus (denv) is a mosquito-borne disease caused by four genetically and serologically related viruses termed denv-1, -2, -3 and -4. the denv envelope (e) protein ectodomain can be divided into three structural domains designated ed1, ed2 and ed3. the ed3 domain contains denv type-specific and denv complex-reactive antigenic sites. to date, nearly all antigenic studies on the e protein have focused on denv-2. in this study, the epitope recognized by a denv-3 type-specific monoclonal antibo ... | 2010 | 20444995 |
| molecular and virological analyses of dengue virus responsible for dengue outbreak in east timor in 2005. | a severe dengue outbreak occurred in east timor in 2005. the dengue virus genome was detected by taqman rt-pcr in 40 serum samples, as follows: dengue virus type-3 (denv-3) in 37 samples, denv-2 in 2 samples, and denv-1 in one sample. one denv-1 genome, one denv-2 genome, and 5 denv-3 genomes were sequenced, and these specimens were aligned with the previously determined envelope (e) gene sequences. the denv-1 strain belonged to genotype iv and was close to those previously isolated in indonesia ... | 2010 | 20495269 |
| profile of time-dependent vegf upregulation in human pulmonary endothelial cells, hpmec-st1.6r infected with denv-1, -2, -3, and -4 viruses. | in this study, the upregulated expression level of vascular endothelial growth factor (vegf) in a pulmonary endothelial cell line (hpmec-st1.6r) infected with dengue virus serotypes 1, 2, 3, and 4 (denv-1, -2, -3 and -4), was investigated. this cell line exhibits the major constitutive and inducible endothelial cell characteristics, as well as angiogenic response. infection by all four denv serotypes was confirmed by an observed cytopathic effect (cpe), as well as rt-pcr (reverse-transcription p ... | 2009 | 19419573 |
| dengue virus 3 genotype i in aedes aegypti mosquitoes and eggs, brazil, 2005-2006. | dengue virus type 3 genotype i was detected in brazil during epidemics in 2002-2004. to confirm this finding, we identified this virus genotype in naturally infected field-caught aedes aegypti mosquitoes and eggs. results showed usefulness of virus investigations in vectors as a component of active epidemiologic surveillance. | 2010 | 20507754 |
| intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice. | dengue, one of the most important arboviral diseases of humans, may cause severe systemic disease. although dengue virus (denv) has been considered to be a non-neurotropic virus, dengue infection has been associated recently with a series of neurological syndromes, including encephalitis. in this work, we evaluated behavioral changes and inflammatory parameters in c57bl/6 mice infected with non-adapted dengue virus 3 (denv-3) genotype i. | 2011 | 21388530 |
| dengue infection increases the locomotor activity of aedes aegypti females. | aedes aegypti is the main vector of the virus causing dengue fever, a disease that has increased dramatically in importance in recent decades, affecting many tropical and sub-tropical areas of the globe. it is known that viruses and other parasites can potentially alter vector behavior. we investigated whether infection with dengue virus modifies the behavior of aedes aegypti females with respect to their activity level. | 2011 | 21408119 |
| evaluation of two molecular methods for the detection of yellow fever virus genome. | yellow fever virus (yfv), a member of the family flaviviridae, genus flavivirus is endemic to tropical areas of africa and south america and is among the arboviruses that pose a threat to public health. recent outbreaks in brazil, bolivia, and paraguay and the observation that vectors capable of transmitting yfv are presenting in urban areas underscore the urgency of improving surveillance and diagnostic methods. two novel methods (rt-hemi-nested-pcr and sybr(®) green qrt-pcr) for efficient dete ... | 2011 | 21419803 |
| dengue virus type 3 isolated from a fatal case with visceral complications induces enhanced proinflammatory responses and apoptosis of human dendritic cells. | a recent (2007 to 2009) dengue outbreak caused by dengue virus (denv) in paraguay presented unusual severe clinical outcomes associated with 50% mortality rates. although it has been reported that inflammatory responses influence the severity of dengue virus infection (t. pang, m. j. cardosa, and m. g. guzman, immunol. cell biol. 85:43-45, 2007), there remains a paucity of information on virus-innate immunity interactions influencing clinical outcome. using human dendritic cells from a major inn ... | 2011 | 21450836 |
| recombinant dengue virus type 3 envelope domain iii protein from escherichia coli. | dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. the envelope protein of dengue virus is the major antigen to elicit neutralizing antibody response and protective immunity in hosts. optimization of culture media was carried out for enhanced production of recombinant dengue virus type 3 envelope domain iii (rden 3 ediii) protein in e. coli. further, batch and fed-batch cultivation process were also develop ... | 2011 | 21538895 |
| genotype v japanese encephalitis virus is emerging. | japanese encephalitis (je) is a global public health issue that has spread widely to more than 20 countries in asia and has extended its geographic range to the south pacific region including australia. je has become the most important cause of viral encephalitis in the world. japanese encephalitis viruses (jev) are divided into five genotypes, based on the nucleotide sequence of the envelope (e) gene. the muar strain, isolated from patient in malaya in 1952, is the sole example of genotype v je ... | 2011 | 21750744 |
| distribution and phylogenetic comparisons of a novel mosquito flavivirus sequence present in culex tarsalis mosquitoes from western canada with viruses isolated in california and colorado. | in a previous study, a new flavivirus genome sequence was identified in culex tarsalis mosquitoes obtained in alberta, canada and was shown to be genetically related to but distinct from members of the insect-specific flaviviruses. nonstructural protein 5-encoding sequences amplified from cx. tarsalis pools from western canada have shown a high similarity to genome sequences of novel flaviviruses isolated from mosquitoes in california and colorado. despite wide distribution of this virus, design ... | 2011 | 21734143 |
| capture of dengue virus type 3 using anionic polymer-coated magnetic beads. | dengue virus (denv) is a mosquito-borne virus and can be transmitted to humans by mosquito vectors. although surveillance of dengue virus-infected mosquitoes is the most effective way of controlling denv infections, detection of denvs in mosquitoes is limited by the low sensitivity of available detection methods. we here report a method for capturing denv type-á3 (denv-3) from mosquito cells using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). the beads ... | 2011 | 21720701 |
| full-length dengue virus rna-dependent rna polymerase - rna/dna complexes. stoichiometries, intrinsic affinities, cooperativities, base, and conformational specificities. | abstract fundamental aspects of interactions of the dengue virus type 3, full-length polymerase with the single-stranded and double-stranded rna, and dna have been quantitatively addressed. the polymerase exists as a monomer with an elongated shape in solution. in the absence of magnesium, the total site-size of the polymerase - ssrna complex is ~26 nucleotides. in the presence of mg2+, the site-size increases to ~29 nucleotides, indicating that magnesium affects the enzyme global conformation. ... | 2011 | 21725087 |
| unusual dengue virus 3 epidemic in nicaragua, 2009. | the four dengue virus serotypes (denv1-4) cause the most prevalent mosquito-borne viral disease affecting humans worldwide. in 2009, nicaragua experienced the largest dengue epidemic in over a decade, marked by unusual clinical presentation, as observed in two prospective studies of pediatric dengue in managua. from august 2009-january 2010, 212 dengue cases were confirmed among 396 study participants at the national pediatric reference hospital. in our parallel community-based cohort study, 170 ... | 2011 | 22087347 |
| [biological properties of virus dengue-3 strains isolated during the epidemic ocurred in havana, 2001-2002]. | during dengue epidemics in cuba, an increase in clinical severity with the epidemics progression in time, particularly in secondary infections, have been frequently observed. it is considered that this increase could be related with genetic changes in the circulating virus. | 2012 | 23444637 |
| la crosse virus infection alters blood feeding behavior in aedes triseriatus and aedes albopictus (diptera: culicidae). | the effects of la crosse virus (lacv) infection on blood feeding behavior in aedes triseriatus (say) and aedes albopictus (skuse) were investigated in the laboratory by measuring the size of the bloodmeal imbibed and the extent of refeeding by virus-infected and uninfected mosquitoes. lacv-infected ae. triseriatus and ae. albopictus took significantly less blood compared with uninfected mosquitoes. twice as many virus-infected ae. triseriatus mosquitoes refed compared with uninfected individuals ... | 2012 | 23270172 |
| long noncoding rna turnover. | most rnas transcribed in mammalian cells lack protein-coding sequences. among them is a vast family of long (>200 nt) noncoding (lnc)rnas. lncrnas can modulate cellular protein expression patterns by influencing the transcription of many genes, the post-transcriptional fate of mrnas and ncrnas, and the turnover and localization of proteins. given the broad impact of lncrnas on gene regulation, there is escalating interest in elucidating the mechanisms that govern the steady-state levels of lncrn ... | 2015 | 25769416 |
| thermodynamic characterization of tandem mismatches found in naturally occurring rna. | although all sequence symmetric tandem mismatches and some sequence asymmetric tandem mismatches have been thermodynamically characterized and a model has been proposed to predict the stability of previously unmeasured sequence asymmetric tandem mismatches [christiansen,m.e. and znosko,b.m. (2008) biochemistry, 47, 4329-4336], experimental thermodynamic data for frequently occurring tandem mismatches is lacking. since experimental data is preferred over a predictive model, the thermodynamic para ... | 2009 | 19509311 |
| dengue virus type-3 envelope protein domain iii; expression and immunogenicity. | production of a recombinant and immunogenic antigen using dengue virus type-3 envelope protein is a key point in dengue vaccine development and diagnostic researches. the goals of this study were providing a recombinant protein from dengue virus type-3 envelope protein and evaluation of its immunogenicity in mice. | 0 | 25691924 |
| dengue virus type 3, south pacific islands, 2013. | after an 18-year absence, dengue virus serotype 3 reemerged in the south pacific islands in 2013. outbreaks in western (solomon islands) and eastern (french polynesia) regions were caused by different genotypes. this finding suggested that immunity against dengue virus serotype, rather than virus genotype, was the principal determinant of reemergence. | 0 | 24856252 |
| evolution of tertiary structure of viral rna dependent polymerases. | viral rna dependent polymerases (vrdps) are present in all rna viruses; unfortunately, their sequence similarity is too low for phylogenetic studies. nevertheless, vrdp protein structures are remarkably conserved. in this study, we used the structural similarity of vrdps to reconstruct their evolutionary history. the major strength of this work is in unifying sequence and structural data into a single quantitative phylogenetic analysis, using powerful a bayesian approach. the resulting phylogram ... | 2014 | 24816789 |
| duck tembusu virus exhibits neurovirulence in balb/c mice. | duck tembusu virus is a member of the ntaya group in the genus flavivirus. the virus has been responsible for severe duck egg-drop syndrome in china since 2010. its emergence and rapid spread have caused great economic loss for the poultry industry. the epidemiology of the virus infection and the potential threat to public health is of great concern because of the infective and zoonotic nature of flaviviruses. | 2013 | 23941427 |
| mosquito saliva serine protease enhances dissemination of dengue virus into the mammalian host. | dengue virus (denv), a flavivirus of global importance, is transmitted to humans by mosquitoes. in this study, we developed in vitro and in vivo models of saliva-mediated enhancement of denv infectivity. serine protease activity in aedes aegypti saliva augmented virus infectivity in vitro by proteolyzing extracellular matrix proteins, thereby increasing viral attachment to heparan sulfate proteoglycans and inducing cell migration. a serine protease inhibitor reduced saliva-mediated enhancement o ... | 2013 | 24131723 |
| viruses and autophagy. | autophagy is an evolutionarily conserved intracellular process by which bulk cytoplasm is enveloped inside a double-membraned vesicle and shuttled to lysosomes for degradation. within the last 15 years, the genes necessary for the execution of autophagy have been identified and the number of tools for studying this process has grown. autophagy is essential for tissue homeostasis and development and defective autophagy is associated with a number of diseases. as intracellular parasites, during th ... | 0 | 19750559 |
| illustrating and homology modeling the proteins of the zika virus. | the zika virus (zikv) is a flavivirus of the family flaviviridae, which is similar to dengue virus, yellow fever and west nile virus. recent outbreaks in south america, latin america, the caribbean and in particular brazil have led to concern for the spread of the disease and potential to cause guillain-barré syndrome and microcephaly. although zikv has been known of for over 60 years there is very little in the way of knowledge of the virus with few publications and no crystal structures. no an ... | 2016 | 27746901 |
| contribution of intertwined loop to membrane association revealed by zika virus full-length ns1 structure. | the association of zika virus (zikv) infections with microcephaly and neurological diseases has highlighted an emerging public health concern. here, we report the crystal structure of the full-length zikv nonstructural protein 1 (ns1), a major host-interaction molecule that functions in flaviviral replication, pathogenesis, and immune evasion. of note, a long intertwined loop is observed in the wing domain of zikv ns1, and forms a hydrophobic "spike", which can contribute to cellular membrane as ... | 2016 | 27578809 |
| outbreak of viral hemorrhagic fever caused by dengue virus type 3 in al-mukalla, yemen. | investigations were conducted by the authors to explore an outbreak of viral hemorrhagic fever (vhf) reported in 2010 from al-mukalla city, the capital of hadramout in yemen. | 2013 | 23497142 |
| emergence of new lineage of dengue virus 3 (genotype iii) in lucknow, india. | dengue has re-emerged as an important arboviral disease causing significant morbidity. it has become hyperendemic in the indian subcontinent with all four known dengue serotypes circulating. | 0 | 23466682 |
| evaluation of a field-portable dna microarray platform and nucleic acid amplification strategies for the detection of arboviruses, arthropods, and bloodmeals. | highly multiplexed assays, such as microarrays, can benefit arbovirus surveillance by allowing researchers to screen for hundreds of targets at once. we evaluated amplification strategies and the practicality of a portable dna microarray platform to analyze virus-infected mosquitoes. the prototype microarray design used here targeted the non-structural protein 5, ribosomal rna, and cytochrome b genes for the detection of flaviviruses, mosquitoes, and bloodmeals, respectively. we identified 13 of ... | 2012 | 23249687 |
| re-emergence of dengue virus type 3 in canton, china, 2009-2010, associated with multiple introductions through different geographical routes. | endemic dengue virus type 3 (denv-3) infections have not been reported in canton, china, since 1980. in march 2009, denv-3 was isolated for the second time, occurring about 30 years after the previous circulation. in august, 3 other cases emerged. one much larger outbreak occurred again in 2010. to address the origin and particularly to determine whether the outbreaks were caused by the same viral genotype, we investigated the epidemiological and molecular characteristics of the introduction, sp ... | 2013 | 23405138 |
| genetic variation in the 3' untranslated region of dengue virus serotype 3 strains isolated from mosquitoes and humans in brazil. | dengue, a mosquito-borne viral infection caused by one of the four dengue virus (denv) serotypes (denv-1 to 4), replicate alternately on the mosquito vector and human host and are responsible for infections throughout tropical and subtropical regions of the world. in brazil, the disease has become a major public health problem and the introduction of denv-3 in 2000 in rio de janeiro (rj) was associated with severe dengue epidemics. the potential emergence of strains associated with severe diseas ... | 2013 | 23282086 |
| genomic and phylogenetic characterization of brazilian yellow fever virus strains. | globally, yellow fever virus infects nearly 200,000 people, leading to 30,000 deaths annually. although the virus is endemic to latin america, only a single genome from this region has been sequenced. here, we report 12 brazilian yellow fever virus complete genomes, their genetic traits, phylogenetic characterization, and phylogeographic dynamics. variable 3' noncoding region (3'ncr) patterns and specific mutations throughout the open reading frame altered predicted secondary structures. our fin ... | 2012 | 23015713 |
| the epidemiology, virology and clinical findings of dengue virus infections in a cohort of indonesian adults in western java. | dengue has emerged as one of the most important infectious diseases in the last five decades. evidence indicates the expansion of dengue virus endemic areas and consequently the exponential increase of dengue virus infections across the subtropics. the clinical manifestations of dengue virus infection include sudden fever, rash, headache, myalgia and in more serious cases, spontaneous bleeding. these manifestations occur in children as well as in adults. defining the epidemiology of dengue in a ... | 2016 | 26872216 |