| a case of partial cerebellar hypoplasia in a cat. | a case of spontaneous ataxia accompanied by partial cerebellar hypoplasia in a two month old siamese kitten is described. the cause of cerebellar hypoplasia is unknown but the feline panleucopenia virus is number one to be suspected. | 1978 | 662593 |
| cloning and sequence of dna encoding structural proteins of the autonomous parvovirus feline panleukopenia virus. | approximately 80% of the genome of feline panleukopenia virus was cloned into pbr322. this dna included the transcription unit for the major viral mrna species. the nucleotide sequence of the cloned portion of the genome was determined. comparison of the feline panleukopenia virus sequence with the sequences of the parvoviruses minute virus of mice and h-1 revealed considerable homology between the three viruses on both the nucleic acid and protein levels. based on this homology, a model for the ... | 1985 | 2991581 |
| the preliminary use of attenuated feline panleukopenia virus to protect cats against panleukopenia and mink against virus enteritis. | | 1965 | 5893051 |
| characterization of antigenic variation among mink enteritis virus isolates. | three antigenic forms of natural field isolates of mink enteritis virus were revealed with a panel of monoclonal antibodies generated against the closely studied feline panleukopenia virus and canine parvovirus-2 virus. two types (types 2 and 3) were shown to be closely related by agar-gel precipitin tests and by restriction enzyme mapping. however, types 2 and 3 differed from the type 1 isolates in the same tests. in cross-protection studies, inactivated viral vaccines made from any one of the ... | 1984 | 6084433 |
| role of neutrophils in production of early enteritis after oral infection with feline infectious peritonitis virus in passively sensitized kittens. | | 1984 | 6090746 |
| comparative studies of physicochemical and biological properties between canine parvovirus and feline panleukopenia virus. | | 1984 | 6092760 |
| [rapid serological diagnosis of parvovirus infection in dogs. fluorescent serological demonstration of virus-specific igm antibodies]. | rapid diagnostic assay described comprises examination of a single serum sample of the ill dog using indirect immunofluorescence test. the procedure lasts about two hours. the finding of virus-specific humoral igm antibodies serves as evidence for an acute parvovirus infection. 84 dogs presented as patients with enteritis were examined with this method, 48 of them were seropositive for igm antibodies. | 1984 | 6093285 |
| recovery of viral agents from the central nervous system of cats. | isolation of viruses from the central nervous system (cns) of cats was attempted using an explant culture technique and subsequent co-cultivation with crandell feline kidney (crfk) or vero cells. feline syncytia-forming virus was isolated from the cns of 11 of 16 cats where the initial co-cultivation was with crfk cells. feline panleucopaenia virus was isolated from the cns of 2 adult cats. co-cultured cells from the cns of 3 cats contained eosinophilic cytoplasmic and intranuclear inclusions. t ... | 1984 | 6093335 |
| in vitro studies on feline panleucopaenia virus. standardisation of haemagglutination-inhibition test for feline panleucopaenia virus antibody. | standardised procedure for obtaining reproducible haemagglutination-inhibition results for fpv antibody which correlate with serum-neutralization titres was described. optimal conditions were found to be alsevers anticoagulant, pbs/0.05% bsa (ph 6.8) as buffer, especially washed round bottom microplates, determination of maximally sensitive porcine erythrocytes, use of reproducible erythrocyte concentrations, inactivation of serum samples at 56 degrees c for 30 min and serum treatment with koali ... | 1984 | 6099788 |
| much of the increased igg in aleutian disease of mink is viral antibody. | aleutian disease (ad) is caused by a persistent infection of mink with an autonomous parvovirus. chronically infected mink develop widespread plasmacytosis, a marked elevation of their serum igg, and immune complex disease. a substantial fraction of the igg in the serum of mink with aleutian disease may be specifically absorbed by monolayer cell cultures infected with aleutian disease virus. the maximum percentage of absorption of igg found was 81% in a mink with 5.4 g/dl of igg. mink with the m ... | 1984 | 6100982 |
| isolation of a virus closely related to feline panleukopenia virus from dogs with diarrhea. | | 1980 | 6158204 |
| antigenic relationships between canine parvovirus type 2, feline panleukopenia virus and mink enteritis virus using conventional antisera and monoclonal antibodies. | the antigenic relationships between three similar parvoviruses, canine parvovirus type 2 (cpv), feline panleukopenia virus (fpv) and mink enteritis virus (mev) were investigated. antisera against all 3 viruses and monoclonal antibodies (mab) to cpv were prepared and the viruses compared using several serological methods. when conventional sera were used in the hemagglutination-inhibition and agar gel precipitin (agp) tests there were no differences between the cpv viral isolates studied, but ant ... | 1982 | 6180709 |
| canine parvovirus: relationship to wild-type and vaccine strains of feline panleukopenia virus and mink enteritis virus. | canine parvovirus (cpv), feline panleukopenia virus (fplv) and mink enteritis virus (mev) were compared serologically, by determination of their host range in cell cultures, as well as by restriction enzyme analysis. maps of the virus genomes were established using seven different restriction enzymes cutting at a total of 56 sites. mev and fplv gave maps which were identical except for one restriction site. the map of cpv is closely related to those of fplv/mev since their dnas share about 80% o ... | 1982 | 6181186 |
| antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus, and mink enteritis virus. | the antigenic structure and variation of canine parvovirus type-2 (cpv), feline panleukopenia virus (fpv), mink enteritis virus (mev), and a closely related virus of raccoons (rpv) was investigated using a panel of 13 monoclonal antibodies (mab) formed against cpv and 8 mab formed against fpv. each mab both neutralized and inhibited the hemagglutination of the homologous virus. all mab tested immunoprecipitated the two capsid proteins. five mab were specific for the cpv isolates and one reacted ... | 1983 | 6194613 |
| a plaque assay for feline panleukopenia virus. | plaque formation with representative strains of feline panleukopenia virus (fpv) has been obtained using a permanent line of feline kidney cells under agarose overlay. fpv-infected cells appear as white plaques after neutral red staining. plaque size is determined by the extent of cell division in the infected monolayer. fpv assay by the plaque procedure is rapid and gives infectivity titres which exceed those determined by the common inclusion body and immunofluorescent assays of fpv by a facto ... | 1980 | 6243343 |
| virucidal disinfectants and feline viruses. | thirty-five commonly used commercial disinfectants (disinfectants, antiseptics, sanitizers, and detergents) were evaluated for their virucidal activity against three feline viruses; feline viral rhinotracheitis virus (a herpesvirus), feline calicivirus, and feline panleukopenia virus (a parvovirus). disinfectants were diluted as recommended by the manufacturer and were reacted with virus for 10 minutes at room temperature. viruses were separated from disinfectants by gel filtration in special ce ... | 1980 | 6245610 |
| feline rhinotracheitis-calici vaccine and feline rhinotracheitis-calici-panleukopenia vaccine: field evaluation for safety. | | 1980 | 6246668 |
| hemagglutination by canine parvovirus: serologic studies and diagnostic applications. | conditions for canine parvoviral hemagglutination (ha) and hemagglutination-inhibition (hi) reactions were defined. the ha phenomena were used to differentiate canine parvovirus (cpv) from feline panleukopenia virus (fpv), mink enteritis virus (mev), and minute virus of canines. serologic comparisons of the cpv, fpv, and mev by ha-hi and serum-neutralization tests indicated that cpv, fpv, and mev were antigenically similar but were different from minute virus of canines. diagnostic application o ... | 1980 | 6250432 |
| origin of canine parvovirus. | | 1980 | 6254227 |
| unusual sequelae to feline enteritis vaccination in a dog. | | 1980 | 6254228 |
| canine parvovirus: safety and efficacy of attenuated feline panleucopenia vaccine. | | 1980 | 6254234 |
| relationships of canine panleucopaenia (enteritis) and myocarditis paroviruses to feline panleucopaenia virus. | | 1980 | 6254483 |
| vaccination of guide dogs. | | 1980 | 6255664 |
| fpl vaccine and canine parvovirus. | | 1980 | 6255666 |
| fpl vaccine and canine parvovirus. | | 1980 | 6255667 |
| parvovirus infection. | | 1980 | 6258041 |
| canine parvovirus vaccination. | | 1980 | 6258042 |
| [detection of antibodies against the canine parvovirus in healthy dogs with indirect immunofluorescence technique (author's transl)]. | | 1981 | 6260454 |
| antigenic differences between canine parvovirus and feline panleucopenia virus. | | 1980 | 6266119 |
| growth characteristics of feline panleukopenia virus in synchronized kitten kidney cells. | | 1981 | 6270441 |
| prevalence of feline viral antibodies in random-source laboratory cats. | over a period of 1973 to 1979, a serologic survey of virus infections was conducted on feline sera collected in four universities which located in different prefectures; obihiro, saitama, kanagawa and tokyo. a significant hemagglutination-inhibition (hi) antibody titer of 1 : 8 or higher to feline panleukopenia virus (fplv) was detected in 130 (58%) of the 226 sera used. no remarkable difference in the hi antibody prevalence in cats to fplv was recognized by years or localities. of a total of 18 ... | 1981 | 6274676 |
| viral antibody studies of laboratory dogs with diarrheal disease. | viral antibody studies were done on laboratory dogs in an epizootic of gastrointestinal disease. increased hemagglutination-inhibition antibody titers to a parvovirus (pv) antigenically related to feline panleukopenia virus were found in convalescent serum specimens of 78% (20/26) of the affected dogs and in 83% (5/6) of apparently healthy dogs. with one exception, all dogs tested had significant levels of hemagglutination-inhibition antibody to this pv. similar increased antibody titers were fo ... | 1981 | 6275746 |
| antibody response to an inactivated vaccine for rhinotracheitis, caliciviral disease, and panleukopenia in nondomestic felids. | the efficacy of an inactivated vaccine for the prevention of feline viral rhinotracheitis (fvr), feline caliciviral disease (fcvd), and feline panleukopenia (fpl) was tested in 27 nondomestic adult felids from 7 species. the vaccine was given im at the standard domestic cat dose in 19 animals and double this dose in 8 others. the animals were vaccinated either 1, 2, or 3 times. serum-neutralization (sn) antibodies to fvr (mean sn titer, 23) developed in all 15 animals that were previously serone ... | 1981 | 6276346 |
| dog response to inactivated canine parvovirus and feline panleukopenia virus vaccines. | inactivated canine parvovirus (cpv) and inactivated feline panleukopenia virus (fpv) vaccines were evaluated in dogs. maximal serologic response occurred within 1-2 weeks after vaccination. antibody titers then declined rapidly to low levels that persisted at least 20 weeks. immunity to cpv, defined as complete resistance to infection, was correlated with serum antibody titer and did not persist longer than 6 weeks after vaccination with inactivated virus. however, protection against generalized ... | 1982 | 6279359 |
| protozoan and viral infections of feral cats. | identification of protozoan oocysts and serological tests were used to determine the prevalence of infections among 300 mainly adult feral cats in three different habitat types in south-eastern australia. oocysts of isospora rivolta and isospora felis were recovered from 3% and 4% respectively of 300 feral cat samples. haemagglutination inhibition antibody to toxoplasma gondii was detected in 20% of 75 cat sera tested. a high prevalence of specific antibody to feline panleukopaenia virus (79%) a ... | 1981 | 6280665 |
| successful experimental challenge of dogs with canine parvovirus-2. | withholding food from dogs for 24 hours prior to, and for 48 hours following oral challenge with a gut mucosal homogenate of canine parvovirus-2, was a successful means of reproducing gastroenteric signs of canine parvovirus-2 infection. twenty-one of 24 dogs, which had previously received various vaccine preparations of mink enteritis virus or were unvaccinated, and which were starved at challenge, developed soft or liquid feces with large or without large clots of mucus. altered feces were mos ... | 1982 | 6280819 |
| the failure of an inactivated mink enteritis virus vaccine in four preparations to provide protection to dogs against challenge with canine parvovirus-2. | four experimental vaccine preparations comprising a strain of mink enteritis virus inactivated by either formalin or beta-propiolactone, and either adjuvanted or nonadjuvanted, failed to stimulate a consistent serum antibody response in 20 vaccinated dogs and failed to protect all but one of these dogs against oral challenge with canine parvovirus-2. | 1982 | 6280820 |
| mink enteritis in japan. i. isolation and characterization of the causative virus and its pathogenicity in cat. | | 1981 | 6283219 |
| mink enteritis in japan. ii. epidemiology of the disease. | | 1981 | 6283222 |
| field evaluation of a canine parvovirus vaccination program, using feline origin modified live virus vaccine. | antibody titers measured by hemagglutination inhibition testing were determined in previously vaccinated dogs at the time of booster vaccination and 2 weeks later. all vaccines consisted of modified live panleukopenia virus. the booster injection was administered approximately 6 months after the initial parvovirus vaccination series was given. fecal and serum specimens were collected immediately before and 2 weeks after administration of the booster vaccine for hemagglutination and hemagglutinat ... | 1982 | 6284689 |
| raccoons are not susceptible to canine parvovirus. | | 1982 | 6290438 |
| [immunoprophylaxis of parvovirosis in dogs (a review)]. | | 1982 | 6297146 |
| evaluation of a killed feline panleukopenia virus vaccine against canine parvoviral enteritis in dogs. | immunogenic potency of a killed feline panleukopenia virus vaccine against canine parvoviral enteritis in dogs was examined. the vaccine elicited hemagglutination-inhibition antibodies to canine parvovirus (cpv) in all of the 72 dogs which were vaccinated. the vaccine was protective in dogs against both experimentally induced and naturally occurring cpv-induced disease. by statistical analysis, 4 weeks was found to be the optimal spacing between 2 vaccinal doses resulting in hemagglutination-inh ... | 1982 | 6299140 |
| use of modified live feline panleukopenia virus vaccine to immunize dogs against canine parvovirus. | modified live feline panleukopenia virus (fplv) vaccine protected dogs against canine parvovirus (cpv) infection. however, unlike the long-lived (greater than or equal to 20-month) immunity engendered by cpv infection, the response of dogs to living fplv was variable. doses of fplv (snow leopard strain) in excess of 10(5.7) tcid50 were necessary for uniform immunization; smaller inocula resulted in decreased success rates. the duration of immunity, as measured by the persistence of hemagglutinat ... | 1983 | 6299142 |
| immunofluorescence for feline infectious peritonitis virus with trypsin-treated paraffin sections. | | 1983 | 6306316 |
| response of mink, skunk, red fox and raccoon to inoculation with mink virus enteritis, feline panleukopenia and canine parvovirus and prevalence of antibody to parvovirus in wild carnivores in ontario. | mink virus enteritis, feline panleukopenia and canine parvovirus-2 were inoculated separately into groups of raccoon, mink, red fox and striped skunk. raccoons were highly susceptible to mink virus enteritis and feline panleukopenia, with animals developing clinical illness, and several dying within six to ten days of inoculation with lesions typical of parvovirus infection. both viruses were shed in high titre in the feces of infected raccoons, and high antibody titres were stimulated. raccoons ... | 1983 | 6309349 |
| molecular cloning of the aleutian disease virus genome: expression of aleutian disease virus antigens by a recombinant plasmid. | three nonoverlapping segments representing approximately 80% of the 4.8-kilobase pair aleutian disease virus (adv-g) duplex genome were molecularly cloned into either bacteriophage m13mp9 (m13bm2 = 0.07 to 0.15 map unit; m13bm1 = 0.15 to 0.54 map unit) or plasmid puc8 (pbm1 = 0.54 to 0.88 map units). in addition the 0.54- to 0.88-map unit segment of a danish isolate of adv (dk adv) was also cloned into puc8 (pbm2). the recombinant plasmids pbm1 and pbm2 induced expression of several polypeptides ... | 1983 | 6313959 |
| comparison of the viral proteins of canine parvovirus-2, mink enteritis virus and feline panleukopenia virus. | canine parvovirus-2 (cpv-2), mink enteritis virus (mev) and feline panleukopenia virus (fpv) were produced using identical cell culture and purification techniques. the distributions of the haemagglutinating activity of the three different parvoviruses in a cscl gradient were similar with haemagglutinating peaks identified at 1.48-1.49, 1.42, 1.36 and 1.30-1.31 g cm-3. the number and distribution of the viral proteins and the equivalent protein molecular weights are similar for all three viruses ... | 1983 | 6316627 |
| macrotiter assay for coronavirus-neutralizing activity in cats using a canine continuous cell line (a-72). | a heterologous neutralization assay for feline infectious peritonitis virus serology was developed using a single continuous cell line of canine origin, a-72, which is susceptible to cytopathic infection with both transmissible gastroenteritis virus of pigs and canine coronavirus. of several coronavirus isolates tested, the 1-71 isolate of canine coronavirus demonstrated the most effective neutralization by serum and body fluids of cats with histopathologically confirmed feline infectious perito ... | 1983 | 6319813 |
| [serological immune response following vaccination with inactivated parvovirus vaccines in the dog]. | | 1983 | 6320363 |
| autoimmunity in spontaneous myasthenia gravis in dogs. | spontaneous canine myasthenia gravis (mg) mimics the human disease in almost every respect. both dogs reported here exhibited the autoantibodies characteristic of mg, i.e., anti-acetylcholine receptor (anti-achr) and antistriational (astr). fluctuations in the anti-achr titer during spontaneous remission and recurrence of mg in one dog provide support for the concept of a symptomatic threshold titer above which anti-achr must rise before disease signs develop. the increase in the anti-achr titer ... | 1984 | 6325059 |
| inconsistency with parvovirus study. | | 1984 | 6330520 |
| experimental infection of feline panleukopenia virus in specific pathogen-free cats. | | 1983 | 6865168 |
| development of a vaccine incorporating killed virus of canine origin for the prevention of canine parvovirus infection. | a parvovirus of canine origin, cultured in a feline kidney cell line, was inactivated with formalin. three pilot serials were produced and three forms of finished vaccine (nonadjuvanted, single adjuvanted and double adjuvanted) were tested in vaccination and challenge trials. a comparison was also made with two inactivated feline panleukopenia virus vaccines, one of which has official approval for use in dogs. the inactivated canine vaccine in nonadjuvanted, adjuvanted or double adjuvanted form ... | 1982 | 7039811 |
| maternally derived immunity to canine parvovirus infection: transfer, decline, and interference with vaccination. | antibody to canine parvovirus (cpv) was transferred from an immune bitch to her pups through the placenta and colostrum. colostral transfer accounted for approximately 90% of the maternally-derived cpv antibody. after suckling, pups and hemagglutination-inhibition titers that averaged 50% of their dam's titer. maternally derived cpv antibody declined with a half-life of 9.7 days. pups with hemagglutination-inhibition titers greater than or equal to 1:80 were immune to oronasal challenge with vir ... | 1982 | 7056660 |
| epizootic of viral enteritis in dogs in thailand. | an epizootic of enteritis occurred in dogs in thailand during 1979. observations were made on 44 dogs that had clinical signs of enteritis or had a recent history compatible with a clinical diagnosis of enteritis. eight of the 44 dogs died. gross and histopathologic examinations performed on these dogs revealed that the lesions were similar to those described for canine viral enteritis. antigens that agglutinated rhesus macaque rbc were detected in feces from 4 of 20 dogs. cytopathic effects wer ... | 1982 | 7149420 |
| [canine parvovirus infection in a group of beagles (author's transl)]. | an outbreak of canine parvovirus infection in a group of 26 beagles was described. the disease characterized by acute intestinal involvement was highly contagious but fatality was low (1/26). transient leukopenia was recorded in some animals. all the convalescent sera were positive in agar gel diffusion test using feline panleukopenia virus antigen. histopathological examination on fetal dog revealed regressive change associated with formation of amphophilic intranuclear inclusion bodies in the ... | 1981 | 7286068 |
| canine parvovirus infection in south american canids. | canine parvovirus (cpv) infections occurred in 5 of 35 south american canids at the department of conservation (dc), a breeding facility of the national zoological park in front royal, va. the clinical signs were anorexia, lethargy, diarrhea, and vomiting. three of the affected canids survived and had high hemagglutination-inhibition titers to cpv in the recovery period. necropsy of the 2 that died revealed extensive necrosis of the intestinal mucosa; cpv particles were observed by electron micr ... | 1980 | 7451312 |
| parvovirus infection in translocated raccoons. | severe enteritis attributed to parvovirus infection was diagnosed in raccoons (procyon lotor) purchased from a commercial animal dealer for interstate translocation. intestinal lesions included edema, hyperemia, and focal mucosal necrosis. microscopically, intestinal villi were reduced markedly, and there was necrosis of the glandular crypts. intranuclear inclusions were seen in many crypt cells. a parvovirus that was infective for crandell feline kidney cells and primary canine kidney cells was ... | 1980 | 7451313 |
| characterization of the feline host range and a specific epitope of feline panleukopenia virus. | the feline parvovirus subgroup is comprised of viruses isolated from various carnivores, including the dog, cat, mink, raccoon, arctic fox, and raccoon dog. those viruses are > 98% identical in their dna sequences and are very similar antigenically. we have shown that although canine parvovirus (cpv) replicates in numerous feline cell lines in vitro it does not infect cats after parenteral inoculation (u. truyen and c. r. parrish, (1992) j. virol. 66, 5399-5408). here we use recombination mappin ... | 1994 | 7513918 |
| inhibitory effects of recombinant feline interferon on the replication of feline enteropathogenic viruses in vitro. | antiviral activities of a recombinant feline interferon (rfeifn) kt-80 were evaluated against feline enteropathogenic viruses in feline and canine cell lines. sensitivity to antiviral activities of the rfeifn varied with cell types; felis catus whole fetus (fcwf-4) cells were more sensitive than crandell feline kidney cells, but no sensitivity was found for madin-darby canine kidney cells when vesicular stomatitis virus was used as a challenge virus. reductions were generally ifn dose-dependent ... | 1994 | 7515537 |
| evolution of the feline-subgroup parvoviruses and the control of canine host range in vivo. | a related group of parvoviruses infects members of many different carnivore families. some of those viruses differ in host range or antigenic properties, but the true relationships are poorly understood. we examined 24 vp1/vp2 and 8 ns1 gene sequences from various parvovirus isolates to determine the phylogenetic relationships between viruses isolated from cats, dogs, asiatic raccoon dogs, mink, raccoons, and foxes. there were about 1.3% pairwise sequence differences between the vp1/vp2 genes of ... | 1995 | 7609035 |
| panleukopenia-like syndrome of felv caused by co-infection with felv and feline panleukopenia virus. | to study the effect of interferon on feline leukemia virus (felv) infection, 30 specific pathogen free (spf) cats were infected with the apathogenic felv a glasgow. unexpectedly, between 5 and 8 weeks after felv infection, all 19 cats with persistent felv infection but not the felv-negative cats died from a panleukopenia-like syndrome. no feline panleukopenia virus (fplv) antigen was found in feces by latex agglutination, enzyme-linked immunosorbent assay (elisa) or immunoelectron microscopy. no ... | 1995 | 7618258 |
| pathogenesis of feline panleukopenia virus and canine parvovirus. | feline panleukopenia virus (fpv) and canine parvovirus (cpv) are autonomous parvoviruses which infect cats or dogs, respectively. both viruses cause an acute disease, with virus replicating for less than seven days before being cleared by the developing immune responses. the viruses have a broad tropism for mitotically active cells. in neonatal animals the viruses replicate in a large number of tissues, and fpv infection of the germinal epithelium of the cerebellum leads to cerebellar hypoplasia ... | 1995 | 7663051 |
| enhancement and neutralization of feline infectious peritonitis virus infection in feline macrophages by neutralizing monoclonal antibodies recognizing different epitopes. | the interaction between the enhancing and neutralizing activities of three monoclonal antibodies (mabs) (5-6-2, 6-4-2 and 7-4-1) to the spike protein of feline infectious peritonitis virus (fipv) strain 79-1146 was determined using feline macrophages. at a high mab concentration, all of the three mabs completely inhibited the fipv infection at 37 c. however, two of them (6-4-2 and 7-4-1) enhanced fipv infection when either the mab concentration or reaction temperature was lowered. these mabs als ... | 1993 | 7694052 |
| [canine parvovirus: recent knowledge of the origin and development of a viral pathogen]. | canine parvovirus (cpv) is a "new" virus that suddenly emerged in the mid 1970s. antigenetically it is very similar to the long known feline panleukopenia virus (fpv). soon after its appearance cpv was classified as a mutant of fpv. as with all "new" viruses, cpv continues to show active evolution, obvious by the appearance of new antigenic types. interestingly, the new types, designated cpv-2a and cpv-2b, completely replaced the original type. this review summarizes the facts that are known abo ... | 1994 | 7716757 |
| there is nothing permanent except change. the emergence of new virus diseases. | the sudden appearance of apparently new viruses with pathogenic potential is of fundamental importance in medical microbiology and a constant threat to humans and animals. the emergence of a "new" pathogen is not an isolated event, as for instance the frequent appearance of new influenza virus strains demonstrates. often the new virus strains co-circulate with the older strains in a susceptible population, but a replacement of the older strains has been also observed. in rare instances the new v ... | 1995 | 7740750 |
| sensitive detection of canine parvovirus dna by the nested polymerase chain reaction. | a polymerase chain reaction (pcr) for the detection of canine parvovirus (cpv) was developed. to increase the sensitivity and specificity of the reaction, the nested pcr with a double-nested primer pair (inner primer pair) was designed. the sequences of the pcr primer pairs were selected from the conserved region in the cpv vp1/vp2 gene. the pcr with the outer or inner primer pair alone (single pcr) could detect 10 fg of viral replicative form (rf) dna on agarose gel electrophoresis; whereas as ... | 1994 | 7801517 |
| symmetric-strand packaging of recombinant parvovirus luiii genomes that retain only the terminal regions. | luiii is an autonomous parvovirus which encapsidates either strand of its genome with similar efficiency in nb324k cells. two parvoviruses closely related to luiii, minute virus of mice (mvm) and h-1 virus, encapsidate primarily the minus strand of their genome when grown in the same cell type. it has been postulated that an at-rich region unique to luiii is responsible for symmetric encapsidation of plus- and minus-strand genomes by luiii. to address this hypothesis, recombinant luiii-luciferas ... | 1995 | 7884925 |
| detection of canine parvovirus dna in paraffin-embedded tissues by polymerase chain reaction. | canine parvovirus (cpv) is seemingly a 'new' virus which suddenly appeared during the mid-1970's in an epizootic of disease in dogs. the virus is very similar to the feline panleukopenia virus (fpv), and recent studies have underlined the possible emergence of cpv as a variant of a virus from some other carnivore--possibly from fpv (parrish, 1990). several conserved amino-acid changes between cpv and fpv isolates have been defined by cloning and sequencing the capsid-protein gene. an alternative ... | 1994 | 7985432 |
| [detection by pcr of genomic markers in canine parvovirus and feline panleukopenia virus]. | the ns1 and vp1/vp2 genes of canine parvovirus and feline panleukopenia virus were amplified by the polymerase chain reaction (pcr). the restriction cleavage patterns of the amplified dna fragments were compared among these parvoviruses including vaccine strains. differences of the restriction site in the ns1 portions were observed between the vaccine strain and the wild type strain as well as between canine parvoviruses and feline panleukopenia viruses. the restriction patterns of feline panleu ... | 1993 | 8019925 |
| serologic survey and serum biochemical reference ranges of the free-ranging mountain lion (felis concolor) in california. | serum samples from 58 mountain lions (felis concolor) in california (usa) were collected between april 1987 and february 1990. nineteen serum samples were used for serum biochemistry determinations; the ranges were similar to reference values in domestic cats, captive exotic felidae and free-ranging mountain lions. a serological survey was conducted to determine whether antibodies were present against selected infectious agents. fifty-four (93%) of 58 sera had antibodies against feline panleukop ... | 1994 | 8028105 |
| the structure of human parvovirus b19 at 8 a resolution. | empty capsids of the human parvovirus b19, self-assembled in a baculovirus expression system, have been crystallized in a cubic space group p2(1)3 with a = 362 a. in spite of extensive purifications, the crystals diffract x-rays to only 8.0 a resolution. diffraction data were collected using oscillation photography with synchrotron radiation. the orientations of the particles in the unit cell were determined with a self-rotation function and their positions were obtained with an r-factor search ... | 1994 | 8030266 |
| production of mink enteritis parvovirus empty capsids by expression in a baculovirus vector system: a recombinant vaccine for mink enteritis parvovirus in mink. | the vp-2 gene of mink enteritis parvovirus (mev) was amplified by the polymerase chain reaction using mev dna isolated from the faeces of a naturally infected mink. subsequently the vp-2 gene was cloned into a baculovirus expression vector. recombinant baculoviruses were isolated and the mev vp-2 gene product was characterized after expression in sf9 insect cells. the mev vp-2 product had the same size as that reported for the wild-type mev vp-2 protein and was recognized by convalescent sera fr ... | 1994 | 8113722 |
| antigenic and genomic variabilities among recently prevalent parvoviruses of canine and feline origin in japan. | canine parvovirus type 2 (cpv-2) and feline panleukopenia (flp) virus (fplv) are well known and ubiquitous diarrhea-causing pantropic viruses. a "new" antigenic variant of cpv-2 (designated as cpv-2a) has been also prevalent among dogs in japan. in the present study, 24 canine and 8 feline isolates collected during 1987-1991 were compared with 17 cpv-2 or cpv-2a and 7 fplv strains that had been characterized previously. genomic properties were determined by the restriction cleavage patterns of a ... | 1993 | 8128593 |
| [immunopathogenesis of virus diseases of cats and dogs]. | immunopathological reactions may determine the pathogenesis of some viral infections of cats and dogs. three pathomechanisms may aggravate the viral disease or may ultimately cause death. some viruses cause transient or persistent immunosuppression (feline immunodeficiency, feline leukemia, feline panleukopenia virus, canine parvovirus-2, canine distemper virus). in other viral infections cells and tissues are destroyed as a sequela of cell-mediated cytotoxicity reactions (demyelinating encephal ... | 1994 | 8165663 |
| mapping of determinants of the host range for canine cells in the genome of canine parvovirus using canine parvovirus/mink enteritis virus chimeric viruses. | feline panleukopenia virus (fplv), mink enteritis virus (mev) and canine parvovirus (cpv) are more than 98% similar in dna and predicted amino acid sequences, but they show different host-cell specificities; cpv is able to replicate in canine cells in culture, whereas fplv and mev cannot or replicate only to a low titre. to map the genomic region responsible for the host range of cpv in vitro, cpv/mev chimeric viruses were generated by transfecting infectious cpv/mev chimeric plasmids into a cul ... | 1994 | 8207398 |
| use of a feline panleukopenia modified live virus vaccine in cats in the primary-stage of feline immunodeficiency virus infection. | cats in the primary stage of feline immunodeficiency virus (fiv) infection and fiv seronegative cats were vaccinated with a modified live feline panleukopenia virus vaccine (fpv-mlv). the fpv-mlv strain was not pathogenic for fiv seronegative cats, whereas in fiv infected cats it produced severe clinical signs, similar to those typically observed in cats naturally infected with field strains of fpv (fever, diarrhoea, leukopenia). | 1993 | 8237206 |
| delayed hypersensitivity testing as a clinical measure of cell-mediated immunity in the cat. | the purposes of this study were to examine the cell-mediated immune response of the normal cat to the modified live feline viral rhinitis, calicivirus, and parvovirus (fvrcp) vaccine (felocell cvr, norden, lincoln, ne), and to evaluate the intradermal skin test as a clinical measure of the immune response of cats. vaccine and diluent were injected intradermally on the dorsal pinna of 34 normal adult cats. skin thickness measurements, lymphocyte counts, and concanavalin a mitogenesis indices were ... | 1993 | 8256439 |
| two dominant neutralizing antigenic determinants of canine parvovirus are found on the threefold spike of the virus capsid. | the 25-nm diameter parvovirus capsid is assembled from 60 copies of a sequence common to the overlapping vp1 and vp2 proteins. here we examine the epitope specificity's of 28 monoclonal antibodies (mab) prepared against canine parvovirus (cpv), feline panleukopenia virus (fpv), and raccoon-dog parvovirus or blue (arctic) fox parvovirus. comparing the reactivity of those mab with various mab-selected escape mutants, or with natural variants of cpv or mink enteritis virus (mev) which differ at kno ... | 1994 | 8259653 |
| reaction rate in cats vaccinated with a new controlled-titer feline panleukopenia-rhinotracheitis-calicivirus-chlamydia psittaci vaccine. | the safety profile of a new controlled-titer feline panleukopenia-rhinotracheitis-calicivirus-chlamydia psittaci vaccine was compared to that of a currently-marketed vaccine. of particular interest were delayed reactions (previously unreported in the literature in felines) occurring 7 to 21 days after vaccination, and the effect of concurrent vaccinations and cat age on the delayed reaction rate. nineteen hundred twenty-four doses of the new vaccine and 364 doses of the comparison vaccine were a ... | 1993 | 8306654 |
| structure determination of feline panleukopenia virus empty particles. | various crystal forms of the single-stranded dna, feline panleukopenia virus (fpv), a parvovirus, have been grown of both full virions and empty particles. the structure of empty particles crystallized in an orthorhombic space group p2(1)2(1)2(1), with unit cell dimensions a = 380.1 a, b = 379.3 a, and c = 350.9 a, has been determined to 3.3 a resolution. the data were collected using oscillation photography with synchrotron radiation. the orientations of the empty capsids in the unit cell were ... | 1993 | 8392729 |
| [nucleotide sequence and genome structure of mink enteritis virus]. | this paper reports the sequence of the cloned mink enteritis virus (mev) rf dna and its 3' end and 5' end structure predicted. mev genome is near 5064 nucleotides (nt) in length. in its 5' end non-coding region, there are three 51 nt repeated sequences. the nucleotide sequence of mev genome has very high homology with those of canine parvovirus (cpv) and feline parvovirus (fpv), reaching 99.1% and 99.9% respectively in the structure gene region. but they differ notably in 5' end non-coding regio ... | 1993 | 8396953 |
| seroprevalence of infectious disease agents in free-ranging florida panthers (felis concolor coryi). | serum samples obtained from 38 free-ranging florida panthers (felis concolor coryi) in southern florida, march 1978 through february 1991, were tested for antibodies against eight bacterial, parasitic, and viral disease agents. sera were positive for antibodies against feline panleukopenia virus (fpv) (78%), feline calicivirus (56%), feline immunodeficiency virus/puma lentivirus (37%), feline enteric coronavirus/feline infectious peritonitis virus (19%), and toxoplasma gondii (9%). all samples w ... | 1993 | 8445789 |
| demonstration of parvovirus in diarrhoeic african cheetahs (acinonyx jubatus jubatus schreber, 1775). | parvovirus was demonstrated in the intestinal content of diarrhoeic african cheetahs by electron microscopy. the virus was isolated in a feline kidney cell line inoculated with a filtrate of the intestinal content. its growth characteristics, cytopathic effect, agglutination of porcine erythrocytes, structure, and results of immunoelectron microscopic examination were indistinguishable from those of feline panleukopenia virus. | 1993 | 8511841 |
| full protection in mink against mink enteritis virus with new generation canine parvovirus vaccines based on synthetic peptide or recombinant protein. | two recently developed vaccine--one based on synthetic peptide and one based on recombinant capsid protein--fully protected dogs against heavy experimental canine parvovirus (cpv) infection. the high sequence homology ( > 98%) and antigenic similarity between cpv and mink enteritis virus (mev), feline panleukopenia virus, and raccoon parvovirus, suggest that both vaccines could protect mink, cats and raccoons against these respective host range variants. this was tested in mink and turned out to ... | 1995 | 8525686 |
| evolution of canine parvovirus involved loss and gain of feline host range. | canine parvovirus (cpv) type-2 emerged as a new virus infecting dogs in 1978, and it was probably derived as a variant of feline panleukopenia virus or of a closely related virus infecting another carnivore. cpv type-2 was subsequently replaced in nature by antigenically variant viruses (cpv type-2a and cpv type-2b) which now coexist in dog populations worldwide. we show that cpv type-2 isolates did not replicate in cats, but that both cpv type-2a and cpv type-2b isolates replicated efficiently. ... | 1996 | 8560765 |
| a simple touch-down polymerase chain reaction for the detection of canine parvovirus and feline panleukopenia virus in feces. | a polymerase chain reaction (pcr) assay is described for the detection of parvovirus in feces of dogs and cats. a touch-down protocol was used which enabled the specific amplification of virion dna from feces after a fast and simple boiling pretreatment. the sensitivity of pcr was as high as ten infectious particles per reaction which corresponds to a titer of about 10(3) infectious particles per gram of unprocessed feces. this renders the pcr about 10- to 100-fold more sensitive than electron m ... | 1995 | 8609207 |
| raccoon poxvirus feline panleukopenia virus vp2 recombinant protects cats against fpv challenge. | an infectious raccoon poxvirus (rcnv) was used to express the feline panleukopenia virus (fpv) open reading frame vp2. the recombinant, rcnv/fpv, was constructed by homologous recombination with a chimeric plasmid for inserting the expression cassette into the thymidine kinase (tk) locus of rcnv. expression of the vp2 dna was regulated by the vaccinia virus late promoter p11. southern blot and polymerase chain reaction (pcr) analyses confirmed the cassette was in the tk gene of the rcnv genome. ... | 1996 | 8615030 |
| feline parvovirus in pedigree kittens. | | 1996 | 8650911 |
| study of hereditary cerebellar degeneration in cats. | to elucidate the nature of ataxia observed in 3 cats spanning 2 generations. | 1996 | 8669758 |
| antigenic type distribution among canine parvoviruses in dogs and cats in germany. | | 1996 | 8737265 |
| species specificity for transduction of cultured cells by a recombinant luiii rodent parvovirus genome encapsidated by canine parvovirus or feline panleukopenia virus. | we previously reported that a recombinant genome derived from the autonomous rodent parvovirus luiii could be pseudotyped with capsids of the closely related viruses, h1 and minute virus of mice. to determine whether this was also possible with less related viruses, luiii recombinant genomes containing a luciferase reporter were cotransfected into permissive cells together with plasmids expressing the capsid proteins of either feline panleukopenia virus (fpv) or its host range variant, canine pa ... | 1996 | 8760427 |
| isolation of canine parvovirus from a cat manifesting clinical signs of feline panleukopenia. | twenty-seven feline parvovirus (fpv) isolates were recovered from cats clinically diagnosed with feline panleukopenia (fpl) for assessing antigenic and genomic properties of fpl viruses (fplv) recently prevalent among cats in japan. all isolates, with the exception of one novel isolate, fpv-314, possessed homologous properties, and their subgroups in fpvs were identified as fplv. the fpv-314 isolate, which was from a 1.5-year-old cat which manifested clinical signs of fpl and died on the 13th da ... | 1996 | 8862565 |
| deep mycotic infections in cats. | a review of deep mycotic infections assessed incidence, signalment, clinical presentation, and outcome in 571 feline cases. pertinent findings included a predisposition in young, male cats for sporothrix schenckii and blastomyces dermatitidis. there was an increased incidence of feline leukemia virus infection in cases with histoplasmosis and of feline panleukopenia virus infection in cases with either aspergillosis or mucoralosis. few other predisposing conditions were identified. cryptococcosi ... | 1996 | 8875352 |
| prevalence of antibodies to feline parvovirus, calicivirus, herpesvirus, coronavirus, and immunodeficiency virus and of feline leukemia virus antigen and the interrelationship of these viral infections in free-ranging lions in east africa. | while viral infections and their impact are well studied in domestic cats, only limited information is available on their occurrence in free-ranging lions. the goals of the present study were (i) to investigate the prevalence of antibodies to feline calicivirus (fcv), herpesvirus (fhv), coronavirus (fcov), parvovirus (fpv), and immunodeficiency virus (fiv) and of feline leukemia virus (felv) antigen in 311 serum samples collected between 1984 and 1991 from lions inhabiting tanzania's national pa ... | 1996 | 8877134 |
| detection of canine parvovirus antigens with antibodies to synthetic peptides. | antibodies produced in rabbits against an 18-amino acid peptide (peptide 1, nslpqsegatnfgdigvp) of capsid protein vp2/residues 292-309 of canine parvovirus (cpv) or against an 18-amino acid peptide (peptide 2, gkrntvlfhgpastkgks) of nonstructural protein ns1/residues 391-409 of cpv identified, in immunofluorescence analysis, viral antigens in canine a 72 cells infected with cpv. antibodies to peptide 2 also identified viral antigens in bovine cells infected with bovine parvovirus. in western blo ... | 1996 | 8893795 |
| structural analysis of a mutation in canine parvovirus which controls antigenicity and host range. | a single mutation in canine parvovirus (cpv) of vp2 residue 300 from alanine to aspartic acid causes a loss of canine host range and alters the antigenic properties of the virus. the three-dimensional structure of this mutant has been solved to 3.25 a resolution. crystals of full particles were triclinic, with cell dimensions of a = 267.6, b = 268.5, c = 274.3 a. alpha = 61.9, beta = 62.6, and gamma = 60.2 degrees. the native structure of cpv was used as an initial model. phases were improved by ... | 1996 | 8918534 |
| differentiation of vaccine virus from field isolates of feline panleukopenia virus by polymerase chain reaction and restriction fragment length polymorphism analysis. | in an attempt to distinguish feline panleukopenia virus (fplv) live vaccine strains from fplv field isolates in japan, we compared restriction fragment length polymorphisms (rflp) of polymerase chain reaction (pcr)-amplified fragments of live fplv vaccine strains with those of fplv japanese field isolates. on the basis of nucleotide sequence differences between pli-iv, a live vaccine strain, and fpv-483, a recent field isolate, two restriction enzymes, dra i and afa i, were selected for pcr-rflp ... | 1996 | 9008339 |
| plant-derived vaccine protects target animals against a viral disease. | the successful expression of animal or human virus epitopes on the surface of plant viruses has recently been demonstrated. these chimeric virus particles (cvps) could represent a cost-effective and safe alternative to conventional animal cell-based vaccines. we report the insertion of oligonucleotides coding for a short linear epitope from the vp2 capsid protein of mink enteritis virus (mev) into an infectious cdna clone of cowpea mosaic virus and the successful expression of the epitope on the ... | 1997 | 9062924 |