| adenovirus-related rna sequences in human neurogenic tumours. | thirty two human tumours, mainly neurogenic, have been investigated for the presence of adenovirus-related rna sequences. 3h-labelled tumour virus dna probes derived from human adenoviruses types 2 and 12, bovine adenovirus type 3, and avian adenovirus celo were hybridized in-situ on tumour kryostat sections under conditions that detect complementary rna. tumour virus-related rna was detected in 62% of all tumours tested, but was not detectable in normal human brain tissues. expression of tumour ... | 1982 | 6278813 |
| a common sequence in the inverted terminal repetitions of human and avian adenoviruses. | the termini of the avian chick embryo lethal orphan (celo) virus dna have been sequenced. the results revealed a 63-bp-long inverted terminal repetition (itr) which shared the sequence ataata with all adenovirus termini, thus far analyzed. the celo virus itr differed from those of the mammalian adenoviruses in two major aspects: (i) it is not a perfect duplication; (ii) it begins with a 5'-guanylic acid residue instead of the cytidylic acid normally observed in adenoviruses. | 1982 | 6290323 |
| biochemical studies of celo virus: an oncogenic avian adenovirus. | | 1983 | 6297961 |
| comparative sequence analysis of the inverted terminal repetition in the genomes of animal and avian adenoviruses. | the nucleotide sequences at the inverted terminal repetitions from two animal adenoviruses, infectious canine hepatitis virus and equine adenovirus, and from one avian adenovirus, celo, were analyzed. dnas from infectious canine hepatitis virus and equine adenovirus contain a homologous region which is 23 nucleotides long from the terminus. the first 17 nucleotides of this region are identical to the ones in human adenovirus type 2 dna. the striking homologous sequence of 14 nucleotides, conserv ... | 1983 | 6301151 |
| a comparison of the terminal protein and hexon polypeptides of avian and human adenoviruses. | it was found that the virion terminal protein of chick embryo lethal orphan (celo) virus had a molecular weight of 46,000, and the hexon a molecular weight of 100,000. 125i-labelled tryptic and chymotryptic peptide maps of the hexons and terminal proteins from celo virus and human adenovirus type 5 (ad5) differed. however, limited proteolysis of celo virus and ad5 terminal proteins by protease v8 showed similarities which were not detected in the case of the two hexons. | 1983 | 6304239 |
| [amplification of virocellular sequences avian adenovirus-induced tumor cells]. | adenovirus-induced tumor cells (including transformed cells) examined contain a part of the viral genome. however, number of molecules of the virus dna persisting in a cell varies in the tumor cell lines. tumor cell lines which were induced by celo virus, an avian adenovirus, carried the virus dna in the range from 1 to 920 molecules per cell. of the tumor cell line which carried large numbers of molecules of the virus dnr, one tumor cell line, rt 13 (160 molecules/cell), was studied both at the ... | 1984 | 6324694 |
| [serological study of a breed of broilers with edema disease]. | twenty selected groups of chickens (835 birds on the whole) coming from randomly chosen broiler production cycles were studied for the occurrence of precipitation antibodies to the viruses of marek's disease, infectious bronchitis, newcastle disease, infectious bursitis, reovirus, tipton virus and celo. the occurrence of these antibodies was subjected to statistical evaluation and to comparison with the occurrence rate of oedema disease in the tested broiler production cycles (296 166 birds on t ... | 1983 | 6414151 |
| alcaligenes faecalis rhinotracheitis in manitoba turkeys. | an outbreak of alcaligenes rhinotracheitis occurred on one premises housing five turkey flocks totaling 25,000 poults. prominent findings were severe respiratory difficulty resulting from excess mucus in the nasopharynx, lachrimation, and tracheal collapse. sinus and tracheal cultures consistently yielded alcaligenes faecalis. an adenovirus was isolated and four flocks became positive for celo virus by agar-gel-precipitin (agp) tests. mortality by flocks ranged from 4% to 48%. treatment was unsu ... | 1984 | 6525132 |
| the avian adenovirus penton: two fibres and one base. | the penton capsomer of mammalian adenoviruses consists of a trimeric, long and thin fibre inserted into a pentameric base. the avian adenoviruses possess a penton which presents another symmetry mismatch: each pentameric base is associated with two fibres. here we have studied the morphology of the penton of celo virus, an avian adenovirus, and we have determined the sequence of both fibres, one long and one short. the short fibre is probably associated with the base in the same way as the mamma ... | 1995 | 7563058 |
| the fowl adenovirus type 1 (celo) virus-associated rna-encoding gene: a new ribozyme-expression vector. | a new system for hammerhead ribozyme (rz) expression was examined in which fowl adenovirus type 1 (celo) virus-associated rna (celo va rna) was used as a vector for the incorporation of rz to target the mrna of secreted alkaline phosphatase (seap) both in vitro and in vivo. the rz gene was integrated into the celo va rna between the internal promoter boxes a and b; apparently this did not interfere with its transcription. rz integrated into celo va rna and, lacking the viral sequences, exhibited ... | 1995 | 7665077 |
| molecular characterization of two strains of the avian adeno-associated virus (aaav). | an avian adeno-associated virus (aaav) was isolated after propagating a field isolate of the celo virus (fowl adenovirus serotype 1 (fav1)) in embryonated eggs. the isolated dependovirus was compared with the known aaav obtained from the american type culture collection (atcc vr-865). the genomes were analysed by digestion with several restriction endonucleases. although both dnas have the same size, most restriction enzymes produced different restriction patterns. double digests were used to co ... | 1995 | 7733829 |
| long-term episomal gene delivery in human lymphoid cells using human and avian adenoviral-assisted transfection. | human b-lymphoblastoid cells transformed by epstein-barr virus (ebv), a common source of established human cell lines, are generally refractory to standard dna transfection methods. we report here the use of human and chicken adenoviruses for the stable delivery of plasmid dna carrying the latent origin of replication, orip, from ebv into human b-lymphoblastoid cells. long-term hygromycin-resistant transformants of human lymphoblastoid cells belonging to the genetically unstable inherited diseas ... | 1994 | 7873181 |
| chicken adenovirus (celo virus) particles augment receptor-mediated dna delivery to mammalian cells and yield exceptional levels of stable transformants. | delivery of genes via receptor-mediated endocytosis is severely limited by the poor exit of endocytosed dna from the endosome. a large enhancement in delivery efficiency has been obtained by including human adenovirus particles in the delivery system. this enhancement is probably a function of the natural adenovirus entry mechanism, which must include passage through or disruption of the endosomal membrane. in an effort to identify safer virus particles useful in this application, we have tested ... | 1993 | 8099627 |
| physical mapping and homology studies of egg drop syndrome (eds-76) adenovirus dna. | homologous sequences between eds-76 adenovirus strain 127 dna and bovine adenovirus dna were found by the southern blotting technique and no homology with celo virus dna could be detected. these data suggest a genetic similarity between eds-76 virus and bovine adenoviruses. the fragments generated form eds-76 adenovirus dna by eight restriction endonucleases were physically mapped. | 1993 | 8380323 |
| [analysis of rat2 cells, transformed by the celo (fav1) virus, using the polymerase chain reaction]. | dnas extracted from the rat2 cells transformed by the celo virus (strain phelps) have been analyzed by polymerase chain reaction. sequences belonging to the left end of the viral genome (according to ishibashi & yasue, 1984) were not found in the tested dnas. on the other hand, the polymerase chain reaction data have revealed the presence of fragments specific of the opposite end. we conclude that this region may have included the genes responsible for cell transformation, at least as far as phe ... | 1993 | 8394508 |
| comparative analysis of the structure and function of adenovirus virus-associated rnas. | the protein kinase dai is an important component of the interferon-induced cellular defense mechanism. in cells infected by adenovirus type 2 (ad2), activation of the kinase is prevented by the synthesis of a small, highly ordered virus-associated (va) rna, va rnai. the inhibitory function of this rna depends on its structure, which has been partially elucidated by a combination of mutagenesis and rnase sensitivity analysis. to gain further insight into the structure and function of this regulat ... | 1993 | 8411363 |
| the complete dna sequence and genomic organization of the avian adenovirus celo. | the complete dna sequence of the avian adenovirus chicken embryo lethal orphan (celo) virus (fav-1) is reported here. the genome was found to be 43,804 bp in length, approximately 8 kb longer than those of the human subgenus c adenoviruses (ad2 and ad5). this length is supported by pulsed-field gel electrophoresis analysis of genomes isolated from several related fav-1 isolates (indiana c and ote). the genes for major viral structural proteins (illa, penton base, hexon, pvi, and pviii), as well ... | 1996 | 8627769 |
| identification of a novel antiapoptotic protein, gam-1, encoded by the celo adenovirus. | we have developed a simple screening method to identify genes that mimic bcl-2 or adenovirus e1b 19k in enhancing cell survival after transfection and have used this method to identify such a gene in the avian adenovirus celo. the gene encodes a novel 30-kda nuclear protein, which we have named gam-1, that functions comparably to bcl-2 and adenovirus e1b 19k in blocking apoptosis. however, gam-1 has no sequence homology to bcl-2, e1b 19k, or any other known antiapoptotic proteins and thus define ... | 1997 | 9060680 |
| the oncogenic potential of eleven avian adenovirus strains. | oncogenic potentials of 11 strains of avian adenovirus representing 10 serotypes were investigated in 22 litters of 1-day-old syrian hamsters (mesocricetus auratus). hamsters were inoculated via two routes: subcutaneous dose of 0.12 ml and intracerebral dose of 0.02 ml. six strains, indiana-c (ind-c), chicken embryo lethal orphan (celo), stein, 75-1a, b-3, and a-2, induced fibrosarcomas subcutaneously at the inoculation site. hamsters inoculated intracerebrally with ind-c, celo, stein, b-3, and ... | 1997 | 9087343 |
| selection of a high activity c-erbb-2 ribozyme using a fusion gene of c-erbb-2 and the enhanced green fluorescent protein. | in different types of human cancer there is an overexpression of the c-erbb-2 (her2/neu) oncogene, which is thought to be involved in tumor progression. therefore the c-erbb-2 oncogene is an attractive target for tumor-specific gene therapy. in this report we have characterized a hammerhead ribozyme against the c-erbb-2 mrna with high cleavage activity. to select the optimum sequence, the activity of five hammerhead ribozymes was tested in a cell-free assay. the hammerhead ribozyme recognizing t ... | 1998 | 9476966 |
| transcriptional organization of the avian adenovirus celo. | a detailed map of the transcriptional organization of the celo virus genome was produced. recent computer analysis of celo virus has indicated the presence of 38 putative open reading frames (orfs). this study, based on analysis of the transcriptional products of celo in vitro, confirmed the presence of rnas for 26 of these 38 orfs. all of the results were obtained by cdna isolation or specific reverse transcriptase pcr. observation of orf transcription kinetics postinfection revealed the existe ... | 1998 | 9765475 |
| sequence and transcriptional analysis of terminal regions of the fowl adenovirus type 8 genome. | fowl adenovirus (fadv) type 1, celo strain has no homologies to mastadenovirus e1a, e1b, e3 and e4, which regulate virus gene expression, dna replication and virus-host interaction. similarly, the right 5 kb and left 15 kb ends of celo virus dna are non-homologous to mastadenoviruses. to compare celo virus with another fadv, 7.5 kb of the left and 17 kb of the right ends of fadv type 8 (strain a-2a) were sequenced and nine and 17 open reading frames (orfs), respectively, were found. this fadv-8 ... | 1998 | 9780058 |
| mutational analysis of the avian adenovirus celo, which provides a basis for gene delivery vectors. | the avian adenovirus celo is being developed as a gene transfer tool. using homologous recombination in escherichia coli, the celo genome was screened for regions that could be deleted and would tolerate the insertion of a marker gene (luciferase or enhanced green fluorescent protein). for each mutant genome, the production of viable virus able to deliver the transgene to target cells was monitored. a series of mutants in the genome identified a set of open reading frames that could be deleted b ... | 1999 | 9882345 |
| termination sequence requirements vary among genes transcribed by rna polymerase iii. | rna polymerase iii (pol iii) transcription generally terminates at a run of four or more thymidine (t) residues but some pol iii genes contain runs of t residues that are not recognized as termination signals. here, we investigate the terminal signal requirements that are operative in adenovirus virus-associated (va) rna genes. in the xenopus 5 s rna gene, efficient termination requires the t residues to be in a g+c-rich sequence context, but a run of five t residues in a g+c-rich context does n ... | 1999 | 10024448 |
| inhibition of influenza a virus reproduction by a ribozyme targeted against pb1 mrna. | a ribozyme gene directed at a specific cleavage of mrna coding for pb1 protein, a component of rna-dependent rna-polymerase of influenza a virus, was constructed. the avian adenovirus celo virus-associated rna (va rna celo) promoter and human cytomegalovirus (cmv) promoter were used for the permanent expression of the ribozyme in cell lines. the cells were infected with influenza a virus strains a/singapore/1/57 and a/wsn/33, and the suppression of the virus reproduction and virus-specific prote ... | 1999 | 10333142 |
| application of the polymerase chain reaction to detect fowl adenoviruses. | the possibility of using the polymerase chain reaction (pcr) for the detection of fowl adenoviruses (fadv) was tested. the optimal reaction parameters were evaluated and defined for purified genomic dna of type 8 fowl adenovirus (fadv-8), and then the same conditions were applied for nucleic acid extracted from infected cells. one hundred picograms of purified viral dna, or 250 fadv-8-infected cells, were detected by ethidium bromide staining of the pcr products in agarose gels. the sensitivity ... | 1999 | 10369570 |
| characterization of celo virus proteins that modulate the prb/e2f pathway. | the avian adenovirus celo can, like the human adenoviruses, transform several mammalian cell types, yet it lacks sequence homology with the transforming, early regions of human adenoviruses. in an attempt to identify how celo virus activates the e2f-dependent gene expression important for s phase in the host cell, we have identified two celo virus open reading frames that cooperate in activating an e2f-inducible reporter system. the encoded proteins, gam-1 and orf22, were both found to interact ... | 1999 | 10400747 |
| [early transcription of fav-1 (celo) avian adenovirus genome]. | the patterns of rna from cells infected with avian adenovirus chicken embryo lethal orphan (celo) virus were analyzed by northern hybridization method. early rnas are specifically hybridized with several fragments of the eco-ri restriction map: a (49-81%), b (0.2-20.3%), d (81-92.5%), and e fragments of the bam-hi celo dna restriction map. early rnas were not hybridized with c (37.8-49%), g (31-37.8%), and e (20.3-31%) fragments of the eco-ri dna celo restriction map. at least 19 types of virus- ... | 1998 | 10488533 |
| delivery of avian cytokines by adenovirus vectors. | a fowl adenovirus serotype 8 (fav-8) recombinant was constructed by inserting an expression cassette consisting of the fav major late promoter/splice leader sequences (mlp/sl), the chicken interferon-gamma (chifn-gamma) gene and sv40 polya into sites in the right hand end of the fav-8 genome. one recombinant (a3-13) was constructed by an insertion of chifn-gamma into a 1.3 kilobase pair (kbp) deletion which removed a putative open reading frame (orf) with identity to the celo (fav serotype 1) 36 ... | 2000 | 10717297 |
| increased adiposity in animals due to a human virus. | four animal models of virus-induced obesity including adiposity induced by an avian adenovirus have been described previously. this is the first report of adiposity induced in animals by a human virus. | 2000 | 10951537 |
| activation of heat-shock response by an adenovirus is essential for virus replication. | successful viral infection requires viruses to redirect host biochemistry to replicate the viral genome, and produce and assemble progeny virions. cellular heat-shock responses, which are characterized as elevation and relocalization of heat-shock proteins, occur during replication of many viruses. such responses might be host reactions to the synthesis of foreign protein, or might be irrelevant consequences of the viral need to activate transcription. alternatively, as heat-shock proteins can f ... | 2000 | 11001061 |
| [transcription of anti-apoptotic proteins from celo and eds avian adenoviruses at early stages of infection]. | transcription of e1b region of avian celo and eds adenoviruses was investigated in infection of primary chicken kidney cell culture with these viruses. transcription of gam-1 gene of celo virus and predicted large t antigen of eds virus was observed 4 and 24 h after cell infection. | 2000 | 11186452 |
| construction of avian adenovirus celo recombinants in cosmids. | the avian adenovirus celo is a promising vector for gene transfer applications. in order to study this potentiality, we developed an improved method for construction of adenovirus vectors in cosmids that was used to engineer the celo genome. for all the recombinant viruses constructed by this method, the ability to produce infectious particles and the stability of the genome were evaluated in a chicken hepatocarcinoma cell line (lmh cell line). our aim was to develop a replication-competent vect ... | 2001 | 11333910 |
| defining car as a cellular receptor for the avian adenovirus celo using a genetic analysis of the two viral fibre proteins. | the coxsackievirus and adenovirus receptor (car) is a high affinity receptor used by adenoviruses, including adenovirus type 5 (ad5). the adenovirus fibre molecule bears the high affinity cell binding domain of ad5, allowing virions to attach to car. the avian adenovirus celo displays two fibre molecules on its capsid and it was logical to expect that the cell binding functions of celo might also reside in one or both of these fibres. we had previously shown that the cell binding properties of c ... | 2001 | 11369892 |
| inhibition of duck hepatitis b virus replication by intrahepatic expression of an antiviral resistance gene. | resistance genes coding for inhibitors of hepadnaviral replication, such as ribozymes, antisense rna, and dominant negative mutants have been shown to be effective in transfected hepatoma cells. in vivo studies, however, are not available to date. here we expanded the use of the duck hepatitis b virus (dhbv) model for studying antiviral resistance genes in vivo. animals were experimentally infected by intravenous injection of dhbv-positive serum in ovo. the use of recombinant human adenovirus ty ... | 2002 | 11894145 |
| histone deacetylase 1 inactivation by an adenovirus early gene product. | gam1 is an early gene product of the avian adenovirus celo and is essential for viral replication. gam1 has no homology to any known proteins; however, its early expression and nuclear localization suggest that the protein functions to influence transcription in the infected cell. a determinant of eukaryotic gene expression is the acetylation state of chromosomal histones and other nuclear proteins. we find that gam1 expression increases the level of transcription from a variety of eukaryotic pr ... | 2002 | 11937030 |
| characterization of fowl adenovirus serotype-4 associated with hydropericardium syndrome in chicken. | the polypeptides of three fowl adenovirus-4 (fav-4) field isolates of hydropericardium syndrome from various geographical areas of the country and the standard fav-1 (celo virus) were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and analysed by protein immunoblotting with polyclonal antibodies to fav-4 and fav-1. protein profile analysis of fav-4 isolates revealed similarity of all the eight polypeptides with molecular weight ranging from 20 to 107 kda but differed from ... | 2002 | 12053912 |
| [eukaryotic vectors of celo avian adenovirus genome, carrying gfp and human il-2 genes]. | recombinant celo avian adenoviruses carrying green fluorescent protein (gfp) and and human interleukin-2 (il-2) genes were obtained by homologous recombination in cell culture. the resultant recombinant celo viruses are reproduced in chick embryos in the renal tubular and chorionic allantoic membrane cells. the ability of celo vectors to transduce human and animal cells was studied in vitro (in cell cultures) and in vivo (in laboratory animals). gfp gene delivery and expression in recombinant ce ... | 2002 | 12180023 |
| the adenovirus protein gam1 interferes with sumoylation of histone deacetylase 1. | the adenovirus early gene product gam1 is crucial for virus replication and induces certain cellular genes by inactivating histone deacetylase 1 (hdac1). we demonstrate that gam1 (i) destroys promyelocitic leukemia nuclear bodies, (ii) delocalizes sumo-1 into the cytoplasm and (iii) influences the sumo-1 pathway. in addition, we show that gam1 counteracts hdac1 sumoylation both in vivo and in vitro. sumoylation of hdac1 does not seem to be absolutely required for hdac1 biological activity but is ... | 2002 | 12393750 |
| [delivery of secreted placental alkaline phosphatase (seap) gene in vitro and in vivo as a component of recombinant avian adenovirus (celo)]. | recombinant adenoviruses capable of expressing the gene of secreted placentary alkaline phosphatase (seap) under control of cmv-promoter was obtained on the basis of celo avian adenovirus and human adenovirus-5 (ad5) genomes. the efficiency of the celo vector was determined in experiments with transduction of human (293, a549, and h1299), mouse (b16), and avian (lmh) cell cultures. it was shown in c57bl/6 mice in vivo that seap gene is expressed under conditions of intravenous, intranasal, and i ... | 2002 | 12534266 |
| modulation of p120e4f transcriptional activity by the gam1 adenoviral early protein. | gam1, an early adenoviral celo protein, is required for viral replication. consistent with its ability to inhibit histone deacetylation by hdac1, gam1 activates transcription. in this report, we identify the cellular transcription factor p120(e4f) as a gam1 interaction partner. p120(e4f) is a low-abundance transcription factor that represses the adenovirus e4 promoter. here we demonstrate that p120(e4f) interacts with hdac1 in vivo and in vitro, and that e4f-mediated transcriptional repression i ... | 2003 | 12730668 |
| structural and phylogenetic analysis of adenovirus hexons by use of high-resolution x-ray crystallographic, molecular modeling, and sequence-based methods. | a major impediment to the use of adenovirus as a gene therapy vector and for vaccine applications is the host immune response to adenovirus hexon-the major protein component of the icosahedral capsid. a solution may lie in novel vectors with modified or chimeric hexons designed to evade the immune response. to facilitate this approach, we have distinguished the portion of hexon that all serotypes have in common from the hypervariable regions that are responsible for capsid diversity and type-spe ... | 2003 | 12915569 |
| observations on a chicken embryo lethal orphan (celo) virus. | | 1957 | 13444590 |
| further studies on celo virus: its relationship to the adenovirus group. | | 1964 | 14247360 |
| the effect of a latent celo virus infection in the chicken embryo, on the propagation of newcastle disease and influenza viruses. | | 1965 | 14340906 |
| reannotation of the celo genome characterizes a set of previously unassigned open reading frames and points to novel modes of host interaction in avian adenoviruses. | the genome of the avian adenovirus chicken embryo lethal orphan (celo) has two terminal regions without detectable homology in mammalian adenoviruses that are left without annotation in the initial analysis. since adenoviruses have been a rich source of new insights into molecular cell biology and practical applications of celo as gene a delivery vector are being considered, this genome appeared worth revisiting. we conducted a systematic reannotation and in-depth sequence analysis of the celo g ... | 2003 | 14604445 |
| restoration of p53 tumor-suppressor activity in human tumor cells in vitro and in their xenografts in vivo by recombinant avian adenovirus celo-p53. | human adenovirus (ad) vectors are extensively used as gene transfer vehicles. however, a serious obstacle for the use of these vectors in clinical applications is due to pre-existing immunity to human ads affecting the efficacy of gene transfer. one of the approaches to circumvent host immune response could be the development of vectors based on non-human ads that are able to transduce genes into human cells. in this study, we explored the possibility of using avian ad celo vectors as gene-trans ... | 2004 | 14681700 |
| recombinant avian adenovirus celo expressing the human interleukin-2: characterization in vitro, in ovo and in vivo. | in our study, a recombinant adenovirus based on the avian adenovirus celo genome, has been constructed that contains the human interleukin-2 gene. we have shown the production of biologically active recombinant interleukin-2 in vitro (lmh and 293 cells) and in ovo (chicken embryos) infected with recombinant virus celo-il2. an increase in the median survival time of c57bl/6 mice carrying b16 melanoma tumors has been demonstrated after multiple intra-tumors injections of the recombinant adenovirus ... | 2004 | 15019245 |
| avian adenovirus celo recombinants expressing vp2 of infectious bursal disease virus induce protection against bursal disease in chickens. | to develop a celo virus vector that can induce protection against infectious bursal disease, celo viruses expressing the host-protective antigen vp2 of infectious bursal disease virus (ibdv) were constructed. in the engineered recombinants, the vp2 gene (the 441-first codons of the ibda polyprotein) was placed under the control of the cmv promoter. two positions in the celo genome were chosen to insert the vp2 expression cassette. the recombinants were found apathogenic, when inoculated by diffe ... | 2004 | 15149796 |
| sequence analysis, viral rescue from infectious clones and generation of recombinant virions of the avian adeno-associated virus. | aiming at the generation of a viral-vectored system for gene delivery and vaccination in poultry, the entire genomes of the vr-865 and da-1 strains of the avian adeno-associated virus have been cloned and sequenced. sequence analysis of the clones showed that the genomic distribution of the structural and non-structural protein-coding genes of these viruses is conserved and in agreement with what has been previously described for the primate adeno-associated viruses. amino acid differences betwe ... | 2004 | 15351493 |
| [the celo-ang recombinant avian adenovirus with human angiogenine gene inducing neovascularization in the anterior tibial muscle of rat]. | the celo recombinant avian adenovirus carrying the gene coding the human angiogenine (ang) synthesis was obtained. expression of the angiogenine gene was shown in the lmh cell culture after infection with the celo-ang virus. the ability of celo recombinant adenoviruses to carry out the delivery and expression of alien genes in muscle cells was demonstrated in experiments with laboratory animals (wistar line rats). the induced neovascularization in rat muscles after the animals were administered ... | 2004 | 15597571 |
| avian adenovirus vector celo-tk displays anticancer activity in human cancer cells and suppresses established murine melanoma tumors. | avian adenovirus celo is a novel adenovirus vector system with the advantages of efficient production, high virion stability, and the absence of crossreactivity with ad5-neutralizing antibodies. in this study, we evaluated the anticancer efficacy of a celo vector encoding the herpes simplex virus type 1 thymidine kinase, a prodrug-activating therapeutic gene. vectors carrying the gene for hsv-tk or egfp under the control of the hcmv promoter in place of the "nonessential" region of the celo geno ... | 2005 | 15761475 |
| deletion of open reading frames 9, 10 and 11 from the avian adenovirus celo genome: effect on biodistribution and humoral responses. | in this study, the in vivo effect of the 3.6 kbp deletion of the three open reading frames (orf) 9, 10 and 11 found at the right end of the celo genome was examined. groups of chickens were inoculated oronasally with 10(5)-10(7) p.f.u. per animal of wild-type virus and two recombinant celo strains (rcelo) expressing luciferase and secreted alkaline phosphatase (seap). the tissue biodistribution, assessed by pcr, was similar for both wild-type and recombinant viruses. the infectious viral particl ... | 2005 | 15958681 |
| chick embryo lethal orphan virus can be polymer-coated and retargeted to infect mammalian cells. | non-human adenovirus vectors have attractive immunological properties for gene therapy but are frequently restricted by inefficient transduction of human target cells. using chicken embryo lethal orphan (celo) virus, we employed a nongenetic mechanism of polymer coating and retargeting with basic fibroblast growth factor (bfgf-pc-celoluc), a strategy that permits efficient tropism modification of human adenovirus. bfgf-pc-celoluc showed efficient uptake and transgene expression in chick embryo f ... | 2006 | 16355117 |
| relaxed template specificity in fowl adenovirus 1 dna replication initiation. | the fowl adenovirus 1 (fadv-1) isolates phelps and ote are highly similar, but have striking differences in the repeat region of the inverted terminal repeat (itr). whilst the repeat region in ote conforms to the conventional human adenovirus repeat region (5'-catcatc), that of phelps contains guanidine residues at positions 1, 4 and 7 (5'-gatgatg). this implies that the fadv-1 isolates phelps and ote have either distinct template specificity at replication initiation or, alternatively, a relaxe ... | 2006 | 16476976 |
| isolation of a type 1 avian adenovirus (celo) mutant which was nonpathogenic for the chicken embryo. | a mutant isolated from plaque purified type 1 avian adenovirus (celo) differed from the parent virus in embryo pathogenicity and in plaque size. | 1970 | 16557791 |
| a broadly applicable method to characterize large dna viruses and adenoviruses based on the dna polymerase gene. | many viral pathogens are poorly characterized, are difficult to culture or reagents are lacking for confirmatory diagnoses. we have developed and tested a robust assay for detecting and characterizing large dna viruses and adenoviruses. the assay is based on the use of degenerate pcr to target a gene common to these viruses, the dna polymerase, and sequencing the products. | 2006 | 16608526 |
| crystallization of the c-terminal head domain of the avian adenovirus celo long fibre. | avian adenovirus celo contains two different fibres: fibre 1, the long fibre, and fibre 2, the short fibre. the short fibre is responsible for binding to an unknown avian receptor and is essential for infection of birds. the long fibre is not essential, but is known to bind the coxsackievirus and adenovirus receptor protein. both trimeric fibres are attached to the same penton base, of which each icosahedral virus contains 12 copies. the short fibre extends straight outwards, while the long fibr ... | 2006 | 16682773 |
| sequence analysis of the left end of fowl adenovirus genomes. | nucleotide sequence analysis of the left end of the genome of fowl adenoviruses (fadv) representing species group c (fadv-4 and -10), d (fadv-2) and e (fadv-8) were carried out, and the sequence data was compared to those of fadv-1 (fadv-a) and fadv-9 (fadv-d). the viruses were propagated in chicken hepatoma cell line for viral dna isolation. restriction endonuclease analysis was performed followed by hybridization with two dna probes representing the left end of fadv-9. the identified fragments ... | 2006 | 16791424 |
| [the induction of protective immune response in mice vaccinated by recombinant avian adenovirus celo expressing glycoprotein g of the rabies virus]. | the recombinant avian adenovirus celo-gprb expressing glycoprotein g of rabies virus (strain ts-80, arriw&m, pokrov, russia) was used for mice vaccination against rabies. double intramuscular immunization by recombinant celo-gprb adenovirus in a dose 10(9) pfu per mouse caused the induction of virus neutralizing antibodies (vna) synthesis in 78% of mice, while twice repeated intradermal injections of the recombinant adenovirus failed to induce the vna production. the protection level in groups o ... | 2006 | 16941876 |
| effect of fowl adenovirus-1 (ibh isolate) on humoral and cellular immune competency of broiler chicks. | fowl adenovirus-1 (fav-1), isolated from field outbreaks of inclusion body hepatitis (ibh), was administered orally to 3-week-old disease-free broiler chicks. humoral immune competency was evaluated by determining the antibody response of infected chicks to sheep red blood cells (srbc) and brucella abortus. fav-1 infection significantly decreased the antibody response of chicks to b. abortus (t-cell-independent antigen) by decreasing igm responses, however, the decreased antibody response to srb ... | 2006 | 17045338 |
| nanoporous crystals of chicken embryo lethal orphan (celo) adenovirus major coat protein, hexon. | celo (chicken embryo lethal orphan) virus is an avian adenovirus that is being developed as a gene transfer vector. its trimeric major coat protein (942 residues, 106,709 da) has 42% sequence identity to human adenovirus type 2 (adh2) hexon and 45% to adh5 hexon. for structural studies, the growth of celo virus has been optimized, and its hexon purified and crystallized. the hexon crystals, the first non-human example, diffract to 3.9 a resolution. molecular replacement using the adh5 model was ... | 2007 | 17071105 |
| kinetic and biologic properties of recombinant chifn-gamma expressed via celo-virus vector. | in this study, a replicative fowl adenovirus serotype 1 (celo) recombinant expressing chicken interferon-gamma (chifn-gamma) was constructed. in the engineered recombinant, the chifn-gamma gene was placed under the control of cytomegalovirus (cmv) promoter. the chifn-gamma expression cassette was inserted in the right end of the celo genome (d fragment), which was able to carry the largest insertion of foreign dna without affecting the replication functions of the vector. the recombinant chifn-g ... | 2007 | 17316138 |
| gam1-associated alterations of drug responsiveness through activation of apoptosis. | an early gene product, gam1, encoded by the avian adenovirus celo, is an inhibitory protein for the sumoylation machinery, which has been implicated in regulating a variety of cellular pathways. in this study, we found that gam1 effectively suppressed both constitutive and inducible sumoylation and caused significant cell growth inhibition. this gam1-mediated cell growth inhibition was associated with induction of apoptosis. in particular, gam1 induced caspase-3 activity as detected by immunosta ... | 2007 | 17575111 |
| identification of hi-like loop in celo adenovirus fiber for incorporation of receptor binding motifs. | vectors based on the chicken embryo lethal orphan (celo) avian adenovirus (ad) have two attractive properties for gene transfer applications: resistance to preformed immune responses to human ads and the ability to grow in chicken embryos, allowing low-cost production of recombinant viruses. however, a major limitation of this technology is that celo vectors demonstrate decreased efficiency of gene transfer into cells expressing low levels of the coxsackie-ad receptor (car). in order to improve ... | 2007 | 17596306 |
| structure of the c-terminal head domain of the fowl adenovirus type 1 long fiber. | avian adenovirus celo (chicken embryo lethal orphan virus, fowl adenovirus type 1) incorporates two different homotrimeric fiber proteins extending from the same penton base: a long fiber (designated fiber 1) and a short fiber (designated fiber 2). the short fibers extend straight outwards from the viral vertices, whilst the long fibers emerge at an angle. in contrast to the short fiber, which binds an unknown avian receptor and has been shown to be essential to the invasiveness of this virus, t ... | 2007 | 17698649 |
| [influence of integrin-binding motif (rgd) on attachment and internalization of avian adenovirus celo in mammalian cells]. | recombinant avian adenovirus celo bearing sequence rgd in the structure of a hi-loop of long fiber was designed. experiments in vitro revealed that introduction of rgd-motif into fiber of celo increased the ability of the virus to be attached to a surface of car-negative cells, and raised efficiency of the process of internalization of the virus both in car-positive, and in car-negative cells. | 2008 | 18488447 |
| structure of the c-terminal head domain of the fowl adenovirus type 1 short fibre. | there are more than 100 known adenovirus serotypes, including 50 human serotypes. they can infect all 5 major vertebrate classes but only aviadenovirus infecting birds and mastadenovirus infecting mammals have been well studied. celo (chicken embryo lethal orphan) adenovirus is responsible for mild respiratory pathologies in birds. most studies on celo virus have focussed on its genome sequence and organisation whereas the structural work on celo proteins has only recently started. contrary to m ... | 2008 | 18561970 |
| [production of human recombinant beta-interferon in the avian cell culture]. | the avian recombinant adenovirus of serotype 1 (celo) was obtained. the recombinant adenovirus of serotype 1 (celo) induces expression of human beta-interferon (ib). the expression cassette containing ib gene was placed at the right end of the celo genome under control of hybrid promoter hef-1alpha/htlv. the resulting recombinant adenovirus celo-ib transduced the avian cell culture lmh. the level of production of the recombinant ib was 0.15 micro/ml. the ib protein yield after affine chromatogra ... | 2008 | 18756823 |
| first step in characterization of cis-acting sequences involved in fowl adenovirus 1 (celo) packaging and its effect on the development of a helper-dependent vector strategy. | adenovirus-based vectors are widely developed for potential utilization as vectors in vaccine and gene therapy strategies. we focused on developing a helper-dependent adenoviral (hd-ad) vector for the potential use of celo, a member of the aviadenovirus genus, in avian species vaccination. our aim was to localize sequences which could play an essential role in celo genome encapsidation and, when deleted, was unable to produce viruses to develop a helper celo virus. a panel of 6 mutants with dele ... | 2009 | 18825496 |
| inhibition of the sumo pathway by gam1. | we have previously demonstrated that gam1, an avian adenoviral protein inhibits sumoylation. by counteracting the sumo pathway, gam1 has a significant impact on virus-infected cells, but in isolation the inhibitory effects of the gam1 protein can be exploited to intentionally manipulate the sumo system in vivo or in vitro. here we discuss in detail the techniques we use to inhibit the sumo pathway using the gam1 protein. | 2009 | 19107425 |
| [construction of the vector based on the celo avian adenovirus genome providing enhanced expression of secreted alkaline phosphatase gene in a non-permissive system in vitro and in vivo]. | aviadenovirus celo is a promising vector for gene delivery to eukaryotic cells, including mammalian cells. in the present state of affairs, it gives a chance to apply recombinant adenoviruses celo against animal pathogens. high expression level of transgene consisting of virus vector is necessary to reach protective humoral and t-cell immune answer. one of the approaches to enhance the expression level of transgene consisting of viral vectors is inclusion of addition 5'- and 3'- untranslated reg ... | 2008 | 19172876 |
| production of recombinant human lactoferrin in the allantoic fluid of embryonated chicken eggs and its characteristics. | the human iron-binding protein lactoferrin (hlf) has been implicated in a number of important physiological pathways, including those regulating immune function and tumor growth. in an effort to develop an efficient system for production of recombinant hlf (rhlf) that is structurally and functionally equivalent to the natural protein, we generated a recombinant celo (chicken embryo lethal orphan) avian adenovirus containing an expression cassette for hlf. embryonated chicken eggs were infected w ... | 2009 | 19174190 |
| molecular characterization of fowl adenoviruses isolated from chickens with gizzard erosions. | broiler chickens with clinical signs of uneven growth, depression, and dull feathers were submitted to our laboratory and, at necropsy, lesions in proventriculus, gizzard, and intestines were detected. fowl adenovirus serotype 1 (fadv-1) was isolated from digestive tissues. the virus, assigned as fadv-pl/g068/08, showed 99.5% nucleotide homology and 99.2% amino acid homology in hexon gene with chicken embryo lethal orphan (celo) strain classified as the european reference of fadv-1. one-day-old ... | 2011 | 21489943 |
| rescue of avian adeno-associated virus from a recombinant plasmid containing deletions in the viral inverted terminal repeats. | we have previously reported the complete genome sequence of avian adeno-associated virus (aaav) strain yz-1, isolated from healthy chickens in china. in this study, we describe the successful rescue of infectious virions from a recombinant plasmid containing the genome of yz-1 with deletions in the viral inverted terminal repeats (itrs). the complete genome of yz-1 was cloned into a bacterial plasmid by a modified "a-t" cloning method. six recombinant plasmids were selected for further experimen ... | 2011 | 21947568 |
| whole genome sequencing of fowl aviadenovirus a - a causative agent of gizzard erosion and ulceration, in adult laying hens. | gizzard erosion and ulceration (geu) caused by fowl aviadenovirus serotype 1 (fadv-1) of the species fowl aviadenovirus a (fadv-a) represents an economically important problem in poultry production. the disease affects mostly young chicken broilers or layers before production. in this study, an unusual geu outbreak in a flock of laying hens at 38weeks of age is described. the affected flock showed elevated mortality rates, with the highest number of dead birds appearing between the 39th and 40th ... | 2017 | 27940213 |
| molecular characterization of quail bronchitis virus isolated from bobwhite quail in minnesota. | from 2008 to 2012, 4 separate cases of quail bronchitis virus infection were seen in bobwhite quail (colinus virginianus) raised in minnesota. the quail chicks ranged in age from 5 d to 8 wk and suffered from respiratory distress and elevated mortality. on necropsy, gross lesions consisted of mucus in trachea, congested lungs, caseous air sacculitis, accumulation of chalky white urates on internal organs, necrotic foci in liver, and enlarged spleen. histologic examination revealed fibrinoheterop ... | 2016 | 27418664 |
| infection with an apathogenic fowl adenovirus serotype-1 strain (celo) prevents adenoviral gizzard erosion in broilers. | gizzard erosion in broilers due to an infection with virulent fowl adenovirus serotype 1 (fadv-1) is an emerging disease. although experimental studies were performed, a possible prevention strategy was not reported so far. the present study was set up to determine (i) a possible influence of birds' age at time of inoculation on the pathogenicity of a european fadv-1 field strain (pa7127), (ii) the virulence of a apathogenic fadv-1 strain (celo), and (iii) its capability to protect spf broilers ... | 2014 | 24930982 |
| fatal necrotic tracheitis by aviadenovirus in captive alagoas curassows (pauxi mitu) extinct from the wild. | extinct from nature, captive young alagoas curassows (pauxi mitu) were found agonizing or dead with respiratory disease. intranuclear inclusion bodies were found in the epithelia of the trachea, associated with marked necrotic tracheitis. an aviadenovirus was isolated in chicken eggs and characterized genetically with 99% identity to the fowl aviadenovirus a, as based on the hexon protein gene. this is the first report of respiratory disease caused by aviadenovirus in any cracid species in brazi ... | 2019 | 30663340 |