| comparative effects of host and viral factors on early pathogenesis of marek's disease. | a series of experiments on the early pathogenesis of marek's disease was conducted according to a uniform scheme. in each experiment, there was a single variable-age, genetic strain, or virus strain. virus assays from spleen, buffy coat, and bone marrow, and fluorescent antibody tests on spleen, bursa of fabricius, and thymus were conducted on five birds per group daily from the 3rd through the 10th day postinoculation. from these data, it was apparent that the response could be divided into two ... | 1977 | 194836 |
| lymphoproliferative diseases of fowl: jm-v leukemic lymphoblasts in cell culture. | jm-v leukemic lymphoblasts were established in cell culture. the cultured cells (jm-vlc cells) were transplantable in young chicks and produced a disease indistinguishable from jm-v lymphoblastic leukemia as initiated by whole-blood inoculation. jm-vlc cells maintained a normal female karyotype through 13 passages in rhode island red cockerels. with the use of jm-v antisera and antisera from birds with naturally occurring marek's disease (md), specific antigens were detected on the surfaces of l ... | 1977 | 195070 |
| [multiplication, interference and interferon induction of avian herpes viruses: vaccination against marek's disease. 1. review of literature]. | | 1977 | 195425 |
| [multiplication, interference, and interferon induction of avian herpesviruses. contribution to the vaccination against marek's disease. 2. materials and methods, results, discussion and conclusion]. | | 1977 | 196461 |
| isolation of marek's disease virus dna from infected cells by electrophoresis on polyacrylamide gels. | marek's disease virus dna isolated from the nuclear fraction of infected chicken embryo fibroblasts and sucrose-purified particles was electrophoresed on 3 per cent polyacrylamide gels and was compared in its electrophoretical behaviour with isolated pseudorabies and herpes simplex dna, strain hf. the dna molecules eluted from the gel were identified by their sedimentation coefficient (53--55s) and buoyant density (1.707 g/ml) to be of viral origin. mdv dna molecules were electrophoretically als ... | 1977 | 196577 |
| isolation and identification of marek's disease virus by fluorescent antibody technique. | cell-associated herpesvirus related to marek's disease (md) was isolated from the direct culture of kidney cells of naturally infected chickens at taoyuan or by inoculation of clinical specimens to chick kidney (ck) and chick embryo fibroblast cells. the virus isolates replicated in ck or chick embryo kidney cell cultures were identified to be md by the fluorescent-antibody technique. | 1976 | 196818 |
| dissociation of antiviral and antitumor immunity in resistance to marek's disease. | immunization of chickens either with gluteral-dehyde-inactivated chicken kidney cells infected with marek's disease (md) virus or with glutaraldehyde-inactivated cells of md lymphoma-derived continuous lymphoblastoid cell lines protected against md. the former type of immunity was associated with an immunologic suppression of virus replication and virus antigen production after challenge with virulent virus, but lymphocytes specifically cytotoxic to cells bearing md tumor antigens were not detec ... | 1977 | 197249 |
| antiviral t cell-mediated immunity in marek's disease. | | 1977 | 197421 |
| notes on the mechanism of postvaccination immunity in marek's disease. | the investigated 16th and 45th in vitro passages of non-pathogenic variant 83 of the kekava strain marek's disease virus have led in chickens to resistance to marek's disease by introduction of the above-mentioned virus 14 days before application of pathogenic variant 55 of the kekava strain marek's disease virus. simultaneous administration of both variants of the kekava strains marek's disease virus did not protect chickens from the disease. presence in those variants of the kekava strain mare ... | 1977 | 198688 |
| [mechanism of postvaccinal immunity in marek's disease]. | the apathogenic variant no. 38 of marek's disease virus, kekava strain (mdv-kekava), at the level of the 16th and 45th passages in vitro made chickens resistant to marek's disease when inoculated 14 days before the infection of the chickens with the pathogenic variant no. 55 of mdv-kekava. a simultaneous administration of both variants did not protect the animals against the disease. the occurrence in variants of mdv-kekava of genetic markets manifest upon virus passages in chick embryo fibrobla ... | 1977 | 198968 |
| pathogenicity of low-virulence marek's disease viruses in normal versus immunologically compromised chickens. | to learn whether "low virulence" isolants of marek's disease (md) virus have a low inherent oncogenic potential, 3 isolants (cu-1, cu-2, s-11) were given to genetically susceptible s-strain or p-line birds with or without immunosuppressive treatments. based on lesion incidence and type during an 8-10-week experimental period, embryonal (17-day) bursectomy and neonatal cyclophosphamide (cy) treatment were without effect on cu-1 and cu-2 infections in p-lines. s-11 virus was virulent for p-lines, ... | 1977 | 199152 |
| [structure and function of virus-induced antigens in cultured cells infected with merek's disease and turkey herpes viruses. ii. isolation of intracellular antigens from infected cells (author's transl)]. | virus-induced antigens were isolated from mdv and hvt-infected cells by salt extraction with 3m kcl and purified by con.a chromatography and isoelectric focusing. electrophoretic analysis of 35s-methionine, 3h-fucose and 32p-orthophosphate labeled antigens revealed 7 different polypeptides, two of them containing labeled carbohydrates, and one phospholipid component. the isolated virus-induced antigens from infected cells were identified as serologically active membrane complexes carrying common ... | 1977 | 199830 |
| [study of the synthesis of rous virus-specific rna in chick embryo cells]. | the presence of virus-specific rna in commercial chick embryos and its lack in chick embryos of leukemia-free chicken farm of the ussr ams oncological research center as well as in cell cultures from rif-free chicken infected with marek's disease virus was demonstrated by hybridizationof 3h-dna-product of rous sarcoma virus synthesized in vitro in the presence of actinomycin d with the total preparation of cellular rna. | 1977 | 200014 |
| [study of the vaccinating properties of 2 variants of marek's disease virus]. | the vaccinating properties of two variants of marek's disease virus, kekava strain (mdv-kekava) have been studied. one of them is naturally attenuated, the other has been produced as a result of attenuation of the pathogenic mdv-kekava by long-term passages in chick embryo fibroblast (cef) culture. the vaccinating properties of the naturally attenuated strains (mdv-kekava 83) were first studied in an experiment in which the blood of chicken no. 1683 was used as the vaccine. in paralled. the mdv- ... | 1977 | 200016 |
| immune response and adult mortality associated with the b locus in chickens. | | 1977 | 200123 |
| demyelination in allergic and marek's disease virus induced neuritis. comparative electron microscopic studies. | patterns of demyelination were studied in sciatic nerves, spinal roots and ganglia of chickens afflicted with either marek's disease (md) or experimental allergic neuritis (ean). md was induced in susceptible chicks after hatching by inoculation of the jm strain of md herpes virus. tissues from these chickens were examined 7-83 days after infection. ean was studied 10-21 days after sensitization of 4 week old chickens to emulsions containing human peripheral nerve with complete freund's adjuvant ... | 1977 | 201143 |
| isolation of marek's disease herpesvirus of low pathogenicity from commercial chickens. | | 1977 | 201237 |
| multiplication of turkey herpes virus and marek's disease virus in chick embryo skin cell cultures. | | 1977 | 201676 |
| [effect of verious disinfectants on dust-borne marek's disease virus]. | | 1977 | 203082 |
| pathological changes in chickens inoculated with reticuloendotheliosis-virus-contaminated marek's disease vaccine. | a disease characterized by delayed growth, anemia, abnormal feathers, and leg paralysis occurred among chickens inoculated with marek's disease vaccine over a period from spring to fall in 1974. these chickens were recognized among flocks inoculated with the vaccine produced by two vaccine makers. the affected ones were examined pathologically. gross examination revealed a slight enlargement of peripheral nerves and atrophy of the spleen, thymus, and bursa of fabricius. histopathologically, the ... | 1977 | 203872 |
| dual virus maturation of both pathogenic and apathogenic marek's disease herpesvirus (mdhv) in the feather follicles of dually infected chickens. | infectious cell-free viruses of both pathogenic and apathogenic marek's disease herpesvirus (mdhv) were detected in feather-tip extracts of chickens dually infected with both mdhv isolants either by inoculation or by contact exposure. this indicated concurrent viral maturation of both pathogenic and apathogenic mdhv isolants in the feather-follicle epithelium, resulting in possible double shedding of the two isolants. | 1977 | 204277 |
| contrasting characteristics of marek's disease herpesvirus isolated from chickens with and without avian leukosis virus infection. | marek's disease herpesvirus (mdhv) isolated from chickens free of naturally occurring avian leukosis virus (alv) infection produced characteristic foci in both chicken embryo fibroblast (cef) and chicken kidney cell (ckc) cultures. mdhv-a, which was extracted from the feather follicle epithelium of chickens naturally infected with alv, did not induce cytologic changes in cef cultures, but did cause focus formation in ck cultures. alv was detected in mdhv-a, but not in mdhv preparations. mdhv-a ( ... | 1978 | 204600 |
| inhibition of herpesvirus replication and herpesvirus-induced deoxyribonucleic acid polymerase by phosphonoformate. | phosphonoformate was found to be an inhibitor of the deoxyribonucleic acid polymerase induced by the herpesvirus of turkeys. the apparent inhibition constants were 1 to 3 mum. phosphonoformate was also able to block the replication in cell culture of marek's disease herpesvirus, the herpesvirus of turkeys, and herpes simplex virus. it was as effective as phosphonoacetate. phosphonoformate was not an effective inhibitor of a phosphonoacetate-resistant mutant of the herpesvirus of turkeys nor of i ... | 1978 | 206200 |
| an improved method for extracting cell-free herpesviruses of marek's disease and turkeys from infected cell cultures. | sonic extraction of cell-free marek's disease herpesvirus (mdhv) and turkey herpesvirus (hvt) from infected cell cultures was improved by incorporating sorbitol in the suspending media. yields of cell-free virus of virulent mdhv were significantly increased with 10% sorbitol added to spga-edta buffer. avirulent strains of mdhv and hvt were respectively readily extracted with spga-edta and spga as the suspending medium, and extraction of their cell-free viruses was moderately improved by adding s ... | 1978 | 206250 |
| spontaneously occurring lymphoproliferative disease in ducks. | a naturally occurring lymphoproliferative disease was observed in a domestic flock of muscovy ducks. gross lesions were observed only in one of three ducks necropsied (in the small intestine). microscopic lesions consisted of mononuclear cell infiltrates composed primarily of reticuloendothelial cells, mature lymphocytes, and plasma cells in livers, spleens, small intestines, peripheral nerves, and brains. antibodies to reticuloendotheliosis virus were detected in sera of four ducks, whereas all ... | 1978 | 206252 |
| sensitivity and specificity of the fluorescent antibody technique for detection of infectious laryngotracheitis virus. | the specificity of a fluorescent conjugate to infectious laryngotracheitis virus was examined using chick trachea organ culture or tissue sections infected with other avian viruses (adenovirus, infectious bronchitis, poxvirus, reovirus, newcastle disease virus, marek's disease virus, avian encephalomyelitis and infectious bursal agent) or mycoplasma gallisepticum. confirmation of virus replication in these preparations was obtained by either 1) demonstration of virus titre increase or 2) demonst ... | 1978 | 206327 |
| [significance of virus-neutralizing antibodies in marek's disease of chickens. 1. immunity control following marek's vaccination with turkey herpes virus (thv)]. | | 1978 | 207505 |
| pathogenesis of marek's disease in chickens with maternal antibody. | histopathological lesions were studied in chickens with maternal antibody against md virus at 14-day intervals after their exposure by contact to hprs-16 of md virus in the first-day of life. the first lesions occured 14 days after infection in the liver, kidney, heart, brain and the sciatic nerves. relatively, the most expressive changes were observed between 56th and 70th day after infection. lesions were characterized as light to heavy infiltrations formed by immature and mature pleomorphic m ... | 1978 | 208481 |
| stimulation of chicken spleen cell mitogenic response with turkey herpesvirus vaccine against marek's disease. | | 1978 | 208875 |
| in vitro establishment of marek's disease herpesvirus-transformed productive and nonproductive lymphoblastoid cell lines. | the establishment of two marek's disease herpesvirus-transformed lymphoblastoid cell lines (jmv-1 and jm-1) is reported. cocultivation with chicken embryo fibroblasts, attempts to rescue marek's disease virus from kidney cell cultures, and immunofluorescent staining show that jmv-1 is a nonproductive line and jm-1 is a productive line. | 1978 | 208974 |
| virus-induced atherosclerosis. | of four groups of chickens, two (groups i and ii) were infected with mdv and two were not (groups iii and iv). groups i and iii were fed diets low in lipid, and groups ii and iv were fed cholesterol-supplemented diets. striking grossly visible atherosclerotic lesions were seen in large coronary arteries, aortas, and major aortic branches of infected normocholesterolemic and hypercholesterolemic chickens (groups i and ii). in contrast, grossly visible atherosclerotic lesions were not seen in unin ... | 1978 | 209124 |
| vaccination immunity to selected diseases in chickens fed the androgen analog mibolerone. | chickens fed the androgen analog mibolerone during the first 7 weeks of life regress their bursa of fabricius but can be properly immunized by vaccination against avian pathogens of major economic importance such as newcastle disease virus, infectious laryngotracheitis virus, avian encephalomyelitis virus, infectious bronchitis virus, fowl pox virus, marek's disease virus, and pasteurella multocida, the pathogen causing fowl cholera. these findings on immunocompetence to infectious agents are im ... | 1978 | 209430 |
| a comparative pathogenesis of two mareks disease virus isolates. | the pathogenesis of the jm and ga isolates of marek's disease virus (mdv) infection was compared by studying the sequential tissue distribution of mdv associated antigens with gross and microscopic lesions in infected chickens. with both mdv isolates the initial sites of viral replication were the lymphoid organs, skin, and featers. both the ga and jm isolates were viscerotropic as demonstrated by the presence of mdv antigens with gross and microscopic changes occurring in several visceral organ ... | 1978 | 209437 |
| [testing the immunogenicity of the dermal antigen in marek's disease virus]. | an experiment was performed to study the immunogenicity of the dermal antigen of marek's disease virus, extracted from the skin of 30-day-old chickens, infected with marek's disease virus on the first day of life. three kinds of samples were tested: (1) dermal antigen centrifuged at 10 000 g per 0.5 h, (2) dermal antigen centrifugated at 10 000 g per 0.5 h and 100 000 g per 1 h, (3) dermal antigen treated like sample (2) and partly purified by deae-cellulose chromatography. samples (1) and (2) w ... | 1978 | 209603 |
| biochemical evidence of the nonintegrated status of marek's disease virus dna in virus-transformed lymphoblastoid cells of chicken. | | 1978 | 209622 |
| [immunogenic activity of vaccines against marek's disease]. | | 1978 | 210559 |
| [interaction of marek's disease and rous sarcoma viruses in a mixed infection in chicks]. | | 1978 | 210560 |
| suppression and enhancement of mitogen response in chickens infected with marek's disease virus and the herpesvirus of turkeys. | the kinetics of phytohemagglutinin (pha) response of peripheral blood lymphocytes from chickens infected with oncogenic marek's disease (md) virus (mdv) or nononcogenic herpesvirus of turkeys (hvt) was studied with a whole blood microassay. at about 7 days after inoculation, a depression in pha response was observed in mdv-inoculated resistant line n or susceptible line 7(2) chickens and in hvt-inoculated line 7(2) chickens. all chickens initially regained their pha responsiveness. susceptible c ... | 1978 | 211085 |
| demonstration of marek's disease tumor-associated surface antigen in chickens infected with nononcogenic marek's disease virus and herpesvirus of turkeys. | the presence of marek's disease tumor-associated surface antigen (matsa) was demonstrated on spleen cells from p-line chickens inoculated 5--6 days earlier with herpesvirus of turkeys and sb-1 (a nononcogenic marek's disease virus). antisera against matsa expressed on five marek's disease lymphoblastoid cell lines were able to recognize the matsa present on sb-1-infected spleen cells. no viral membrane antigens and only a low incidence of viral internal antigens could be demonstrated. | 1978 | 211242 |
| fused rocket and rocket immunoelectrophoresis adapted for demonstration and quantitation of the a antigen of marek's disease virus-infected cell cultures. | preliminary results have shown that the rocket immunoelectrophoresis technique can be used as a method for quantitation of the a antigen of marek's disease virus-infected cell cultures. | 1978 | 211484 |
| duration of vaccinal immunity against marek's disease. | specific vaccinal immunity against marek's disease (md) virus was measured by a short-term challenge test in which chickens vaccinated with turkey herpesvirus (hvt) and control chickens at various ages up to 40 weeks were inoculated with virulent md virus and evaluated 2 weeks later for microscopic lesions and md viremia. vaccinal immunity induced by high (2,900 pfu) and low (29 pfu) doses of hvt persisted without detectable reduction through the 40th week in line 151 chickens, thus indicating t ... | 1978 | 211999 |
| lymphoproliferative diseases of fowl: characterization of transplantable g-b1 marek's disease tumor cells in culture. | a transplantable marek's disease tumor, derived and maintained through in vivo passage in syngeneic g-b1 chickens, was established in cell culture and characterized. the cells (gbt cells) grew in suspension and appeared morphologically similar to other marek's lymphoblastoid lines except for prominent nucleoli in gbt cells. the number of chromosomes increased during in vitro cultivation from near triploid to subtetraploid. chromosome no. 3 was abnormally short. the gbt cells retained surface b1 ... | 1978 | 212000 |
| development of cell-mediated immunity to marek's disease tumor cells in chickens inoculated with marek's disease vaccines. | chickens inoculated with herpesvirus of turkeys or with apathogenic or attenuated vaccine strains of marek's disease virus (mdv) developed a t-cell-mediated immune response to marek's disease (md) tumor cells. this immune response was detected in a 4-hour 51cr-release assay in which effector cells obtained from spleens of vaccinated chickens were reacted with 51cr-labeled target cells of an md lymphoblastoid cell line (msb-1). the cytotoxic effector cells generated by the vaccine viruses had cha ... | 1978 | 213610 |
| specific restriction of avian sarcoma viruses by a line of transformed lymphoid cells. | | 1978 | 213875 |
| nonprotective and temperature-sensitive variants of marek's disease vaccine viruses. | | 1979 | 214607 |
| protection against marek's disease-derived tumor transplants by the nononcogenic sb-1 strain of marek's disease virus. | a series of experiments was conducted to study the in vivo protection against marek's disease-derived tumor transplants by the nononcogenic sb-1 strain of marek's disease virus. intact, embryonally bursectomized (bx), thymectomized (tx), or cyclophosphamide (cy)-treated chickens of four genetic lines were vaccinated with live or inactivated sb-1. jmv, a non-virus-producing transplant, and ga/tr-1 and mdt-198, two virus-producing transplants were used for challenge. optimal protection against jmv ... | 1978 | 215543 |
| enhanced oncornavirus expression in marek's disease tumors from specific-pathogen-free chickens. | an oncornavirus was recovered from cell cultures of kidney tumors from specific-pathogen-free chickens inoculated with marek's disease herpesvirus (mdhv). the mdhv inoculum was free of infectious avian leukosis virus (alv). direct examination of a variety of tissues from mdhv-inoculated chickens demonstrated increased levels of alv-specific rna compared to tissues from diluent-inoculated (control) chickens. dna from cultured kidney tumor cells annealed to an alv complementary dna probe at the sa ... | 1979 | 216833 |
| further study on the three-dimensional structure of the core of marek's disease virus and herpesvirus of turkey. | three-dimensional structures of the core of marek's disease virus and herpesvirus of turkey were examined by the tilting apparatus of an electron microscope. various types of the core found in the infected cells were considered to represent developmental stages of the viruses. the basic structure of the core consisted of a toroid surrounding a cylindrical mass, which was clearly demonstrated by tilting the core in two directions. a cylindrical mass spooled by more than two toroids, which seemed ... | 1979 | 218531 |
| no homology detectable between marek's disease virus (mdv) dna and herpesvirus of the turkey (hvt) dna. | the relation of four different strains of mdv and two strains of hvt was analyzed by gel electrophoresis of viral dna digested by various restriction endonucleases and by filter hybridization of viral dna with complementary rna. the four mdv strains showed fragment patterns completely different from those of hvt upon digestion of the viral dna with bam h i, eco r i, hind iii, hpa, i, and xho and separation of fragments on agarose gels. the cleavage patterns of the four mdv strains showed great s ... | 1979 | 219323 |
| [experiments with the simultaneous or combined vaccination of chicks against marek's disease and newcastle disease]. | simultaneous vaccination was carried out of day-old chicks against marek's disease (strain c3--1) and newcastle disease (strains b1 and la sota) which did not prove to be effective in conferring a lasting immunity to newcastle disease though it was tolerated without any disturbances whatever. one could resort to it by way of an exception only, with an obligatory revaccination 15 days later, when the unfavourable epizootic status made it necessary. the immunity built up against marek's disease di ... | 1978 | 219586 |
| [immunomorphological changes in chicks vaccinated against marek's disease with herpesvirus sz-1]. | | 1978 | 219588 |
| transcription of the marek's disease virus genome in a nonproductive chicken lymphoblastoid cell line. | | 1979 | 219594 |
| minor dna homology between herpesvirus of turkey and marek's disease virus? | | 1979 | 219602 |
| effectiveness of cell-free or cell-associated turkey herpesvirus vaccine against marek's disease in chickens as influenced by maternal antibody, vaccine dose, and time of exposure to marek's disease virus. | | 1978 | 219826 |
| in vitro inactivation of cell-free marek's disease herpesvirus by immune peripheral blood lymphocytes. | the effect of peripheral blood lymphocytes (pbl) on cell-free marek's disease virus (mdv) was studied in vitro in pbl from intact or embryonal bursectomized (bx) chicks infected with the apathogenic sb-1 isolate of mdv. the pbl were harvested purified and were mixed, 200 or 2,000 to 1, with cell-free ga-5 of mdv. virus survival was assayed after incubation for 1 hour at 36 c by the standard assay for cell-free mdv. pbl from intact but not bx chickens were able to inactivate virus. when the effec ... | 1978 | 219833 |
| status of endogenous avian rna tumor virus in marek's disease lymphoblastoid cell lines and susceptibility of those lines to exogenous rna tumor viruses. | three lymphoblastoid cell lines from marek's disease (md) tumors, two md virus (mdv) producer lines (msb-1 and hprs-1), and a nonproducer line (rpl-1) were studied for the expression of avian leukosis sarcoma (als) viruses. the msb-1 line was free of all known endogenous expressions, including replicating virus (rav-o), group-specific (gs) antigens, and chick helper factor (chf). the rpl-1 and hprs-1 were positive for gs antigens and chf. the rpl-1 and msb-1 lines showed no evidence of an exogen ... | 1978 | 219836 |
| altered biological and biochemical properties of a phosphonoacetate-resistant mutant of herpesvirus of turkeys. | a phosphonoacetate (pa)-resistant mutant of the herpesvirus of turkeys (hvt) was isolated and characterized. the mutant (hvtpa) replicates in growth medium containing 300 microgram/ml of pa and shows in vitro temperature sensitivity at 41 degrees c (its 37 degrees c/41 degrees c efficiency of replication is about 5). hvtpa replicates poorly in chickens and fails to provide complete protection against mdv challenge. the hvtpa-induced dna polymerase has an apparent inhibition constant for pa 10 ti ... | 1978 | 221331 |
| effect of cyclic nucleotides on the response of cells to infection by various herpesviruses. | the effect of varying the intracellular cyclic nucleotide concentration of mouse cells infected with hsv-1 or of duck embryo fibroblasts infected with marek's disease virus has been studied. it has been shown in each case that agents that elevate the intracellular camp levels increase the yield of infectious virus or viral dna, but inhibit cell division. cgmp enhances synthesis of viral dna but allows continued synthesis of cell dna. | 1978 | 221343 |
| identification and partial purification of two ebv-associated dna polymerases. | virally induced dna polymerases have been demonstrated in cells infected with a variety of herpesviruses. these include herpes simplex virus (weissbach et al., 1973), marek's disease virus (boezi et al., 1974), equine herpesvirus (allen et al., 1977), and cytomegalovirus (huang, 1975a). recently a new iododeoxyuridine (iudr)-induced intracellular dna polymerase in an epstein-barr virus (ebv)-producing hybrid cell line has also been reported (miller et al., 1977). we present evidence that there a ... | 1978 | 221345 |
| transformation of lymphoid cells by herpesviruses: a review with special reference to the phenotypic properties of transformed cells. | | 1978 | 221350 |
| non-protective variants of marek's disease vaccine viruses. | two variant marek's disease-vaccine viruses failed to induce protective immune responses in chickens and replicated poorly in vivo. one of these, mdv/200d, was defective for growth at 41 degrees c, and clones from this stock had 38 degrees c/41 degrees c plating ratios as high as 10(4). the basis for the poor in vivo replicative ability was not determined. | 1978 | 221372 |
| transfection studies in vitro and in vivo with isolated marek's disease virus dna. | for tranfection studies in vitro and in vivo, infectious marek's disease virus (mdv) dna was isolated from purified virus particles. its sedimentation coefficient in neutral sucrose gradients was found to be 53 +/- 2s and the buoyant density in caesium chloride corresponded to 1.707 g/cm3. the virus dna was also analysed by using the restriction endonucleases ecori and hindiii. high molecular-weight mdv dna precipitated by the calcium phosphate technique was shown to be infectious in cultivated ... | 1978 | 221387 |
| expression of marek's disease virus in producer and non-producer lymphoblastoid cell lines. | continuous in vitro propagation of a producer marek's disease (md) lymphoblastoid cell line reduced the number of cells producing virus antigens and the inducibility of these antigens to 5-iododeoxyuridine (iudr) but did not change the oncogenicity of the cells for chickens. in contrast, propagation of a non-producer md cell line did not change the non-productivity of the cell line, but decreased the transplantability of the cells. the md lines were free of replicating avian leukosis sarcoma vir ... | 1978 | 221388 |
| cytotoxicity of lymphocytes from chickens with marek's disease (md) to md tumour cell lines. | | 1978 | 221408 |
| the role of the macrophages in marek's disease: in vitro and in vivo studies. | macrophages form s- and k-strain leghorn chickens, susceptible and restant to marek's disease (md) respectively, were studied to determine the macrophage contribution to the dynamics of md infection, tumorigenesis and genetic resistance to this disease. in vitro studies demonstrated that macrophages from bothstrains were similar in their responses toward jm strain of marek's disease virus (mdv) and jm-1 tumor cells. macrophages were observed to phagocytize jm virus, but the interiorized virus wa ... | 1979 | 222692 |
| lymphomas resembling lymphoid leukosis in chickens inoculated with reticuloendotheliosis virus. | chickens inoculated as embyros or at hatching with the chick syncytial strain of reticuloendotheliosis virus developed a high incidence of lymphoid neoplasms between the 17th and 43rd weeks of age, involving principally the liver and bursa of fabricius. on the basis of organ distribution, latent period, pathology and surface igm production, the lymphomas closely resembled those of lymphoid leukosis. one inoculated chicken developed a myxosarcoma. no tumors were observed in uninoculated controls. ... | 1979 | 222694 |
| expression of genetic resistance to an oncogenic herpesvirus at the target cell level. | | 1979 | 223067 |
| infection studies on a reticuloendotheliosis virus contaminant of a commercial marek's disease vaccine. | reticuloendotheliosis virus (rev) was isolated in cell cultures from commercial marek's disease (herpesvirus of turkeys) vaccine and re-isolated from the organs of vaccinated chickens. runting and feathering abnormalities were produced when 1-day-old specific pathogen free chickens were inoculated with rev. histopathological lesions in infected chickens were hypoplasia of the thymus, bursa and spleen, and inflammation of the proventriculus, kidneys and liver. serological responses to rev were de ... | 1979 | 223539 |
| macrophage restriction of marek's disease virus replication and lymphoma cell proliferation. | macrophages are shown to restrict the replication of marek's disease virus (mdv) and isotope uptake by spleen cells from chickens bearing marek's disease (md) tumors. the titer of virus from duck embryo fibroblasts (def) co-cultivation with mdv-spleen cells pretreated to deplete marcophages was 4- to 18-fold higher than with untreated cells. treated mdv-spleen cells increased isotope uptake by 2-fold. these restrictive activities are attributable to macrophage regulation of cell proliferation. | 1979 | 224109 |
| induction of virus-neutralizing antibody by glycoproteins isolated from chicken cells infected with a herpesvirus of turkeys. | the production of virus-induced proteins in chicken embryo fibroblast cells infected with a herpesvirus of turkeys was studied. it was found that glycoproteins isolated from membrane-rich fractions of infected cells by affinity chromatography using concanavalin a induced neutralizing and precipitating antibody in rabbits and chickens. after analytical electrophoresis, such isolates were found to contain three polypeptide bands of between 100 x 10(3) and 120 x 10(3) molecular weight not present i ... | 1979 | 225277 |
| transcription of the marek's disease virus genome in virus-induced tumors. | transcription of the marek's disease virus (mdv) genome in tumor tissues from mdv-infected chickens has been studied by analyzing the hybridization kinetics of (3)h-labeled mdv dna with unlabeled rna extracted from these tissues. lymphoid tumors of ovary, spleen, liver, and kidney contained mdv genomes, but the virus-specific rna sequences were transcribed from less than 15% of the viral dna. a virus nonproductive lymphoblastoid cell line, designated mkt-1, has been established from a kidney lym ... | 1979 | 225531 |
| [susceptibility of japanese quail cells to virus infections in vitro and in vivo]. | | 1979 | 225762 |
| effects of avian reovirus on marek's disease (md). i. suppression of md development. | the effects of an avian reovirus on the development of acute marek's disease (md) were studied by exposing chickens to reo-w isolant of avian reovirus before infecting them with an oncogenic md herpesvirus (mdhv). four separate trials were made in a similar manner, 2 trials with an experimental line of white leghorn (wsu-vs) highly susceptible to md, and 2 trials with a commercial strain of meat-type (c-mt) chicken. md mortality and/or incidence of gross md lesions were consistently reduced by p ... | 1979 | 226049 |
| complement-dependent antibody cytotoxicity test of chicken antibody with duck complement used against cells of a marek's disease lymphoma-derived cell line (msb-1). | complement-dependent antibody cytotoxicity (cdac) against cells of a lymphoblastoid cell line (msb-1) derived from marek's disease lymphoma was investigated in a chicken antibody system using complements from several animal species. cytotoxicity was seldom observed when rabbit, guinea pig, or chicken complements were used in the presence of hyperimmune chicken serum against msb-1. with duck complement, however, cytotoxicity was always observed. therefore, duck complement appears to be suitable f ... | 1979 | 226054 |
| pathogenesis of marek's disease-distribution of viral antigens in infected chickens as detected by immuno-peroxidase test. | | 1979 | 226476 |
| a comparison of titration methods for marek's disease vaccines. | | 1979 | 226553 |
| depression of vaccinal immunity to marek's disease by infection with reticuloendotheliosis virus. | the effect of infection with low-virulence, tissue culture-propagated strains of reticuloendotheliosis virus (rev) on protective vaccinal immunity against marek's disease (md) lymphomas was investigated. vaccinated chickens inoculated at hatching with greater than 10(4) focus-forming units of rev and challenged with md virus were poorly protected against md lesion development as indicated by protective indices of 53 to 79% for strain cs (p less than 0.05) and 42 to 49% for strain t (p less than ... | 1979 | 227800 |
| inhibitory effects of macrophages against marek's disease virus plaque formation in chicken kidney cell cultures. | inhibition of plaque formation by marek's disease virus (mdv) in chicken kidney cell cultures was investigated with the use of peritoneal exudate cells (pec) from chickens. pec from mdv-infected white leghorn chickens inhibited the formation of mdv plaques, whereas the inhibitory effect of pec from chickens vaccinated with herpesvirus of turkey (hvt) or pec from normal chickens was very weak. however, pec from either normal chickens or hvt-vaccinated chickens inhibited the mdv plaque formation i ... | 1979 | 228107 |
| replication of turkey herpesvirus and marek's disease virus in chick embryo skin organ culture. | turkey herpesvirus (hvt) and an attenuated marek's disease virus (mdv) replicated in organ cultures of chick embryo skin as assessed by immunofluorescence and/or electron microscopy. hvt-specific immunofluorescent antigen was detected in the feather follicle epithelium (ffe) and in the surface layer of the skin epidermis. electron microscopy of infected explants revealed herpes-type cytopathology. immature particles of both viruses appeared first in the nucleus. oval or horseshoe-shaped non-enve ... | 1979 | 228368 |
| marek's disease lymphoma of chicken and its causative herpesvirus. | | 1979 | 228727 |
| turkey herpesvirus and marek's disease virus. a comparative appraisal. | | 1979 | 228905 |
| studies on genetic resistance of chickens to marek's disease--a review. | | 1979 | 228906 |
| comparative pathogenesis studies with oncogenic and nononcogenic marek's disease viruses and turkey herpesvirus. | an apparently nononcogenic marek's disease virus (sb-1) and turkey herpesvirus could be readily isolated from spleen, bursa of fabricius, thymus, and peripheral blood lymphocytes of chickens beginning 4 to 6 days after inoculation, but unlike infections with two isolates of oncogenic marek's disease virus (jm-10 and cu-2), virus replication in these cells was rare, and necrosis in the organs was essentially absent. splenic enlargement was observed regularly during the first 4 to 11 days after in ... | 1979 | 229747 |
| intracellular state of marek's disease virus dna in two tumour-derived chicken cell lines. | | 1979 | 230282 |
| comparative studies on marek's disease virus and herpesvirus of turkey dnas. | dna of marek's disease virus (mdv) was compared to that of herpes virus of turkey (hvt). centrifugation of the two virus dnas in neutral glycerol and cscl density gradients showed that the mdv genome was slightly larger than that of hvt and that the buoyant density (1.705 g/ml) of mdv dna in cscl gradients was slightly lower than that (1.707 g/ml) of hvt dna. mdv and hvt dnas were digested with either ecori or hindiii restriction endonuclease and analysed by 0.5% agarose gel electrophoresis. the ... | 1979 | 230299 |
| increased susceptibility of leukemia-infected chickens to marek's disease. | intramuscular (i.m.) injection of rav-49 into 1--3 day-old chickens infected with marek's disease herpes virus (mdhv) by contact led to exceedingly high rate of marek's disease (md) incidence. according to the data obtained in three experiments 52 out of 108 (48.1%) chickens fell ill in this group. while in the group uninfected with rav-49 but contactly infected with mdv-kekava there were only 11 out of 102, (10.7%) incidences. simultaneous inoculations of rav-49 and mdv had no effect on md morb ... | 1979 | 230419 |
| [avian viral infection with a neoplastic evolution: marek's disease]. | | 1979 | 230584 |
| marek's disease in chickens: correlation of marek's disease with nuclear-inclusion formation in feather-follicle epithelium. | the dynamics of nuclear-inclusion (ni) formation in feather-follicle epithelium of chickens inoculated with marek's disease virus (with or without prior immunization with turkey herpesvirus) could be divided into two patterns: 1) transient ni formation at the initial stage postchallenge; and 2) persistent ni formation. incidence of marek's disease was closely correlated with the dynamics of ni formation. active ni formation recurred in chickens showing pattern 2 and was closely related to the in ... | 1979 | 230801 |
| low incidence of lymphoid tumors in chickens continuously producing endogenous virus. | a flock of 258 male and 243 female chickens of a cross of regional poultry research laboratory lines 15b and 7(2) were kept in a filtered-air positive-pressure house and observed for tumors from 100 to more than 729 days of age. these birds produced high titers of a subgroup e endogenous virus from the middle of the embryonic incubation period through the end of the experiment. no neoplasms were observed in the males. the females had two neoplasms indistinguishable from lymphoid leukosis and thr ... | 1979 | 230806 |
| [the molecular biology of herpes viruses: (part ii) dna structure analysis and latent viral genomes of ebv, mdv, h. saimiri, cmv etc. (author's transl)]. | | 1979 | 231281 |
| effect of culture temperature on the production of marek's disease virus antigens in a chicken lymphoblastoid cell line. | | 1979 | 231431 |
| establishment of marek's disease lymphoblastoid cell lines from chickens with babk of b blood groups. | | 1979 | 231432 |
| marek's disease virus and herpesvirus of turkey noninfective to chickens, obtained by repeated in vitro passages. | the relation between the passage level of marek's disease virus, c2 strain, and of herpesvirus of turkey (hvt), o1 strain, in cell culture and the level of the serological response of chickens to these viruses was examined. in both cases the immune response of chickens to these viruses decreased with increase in the number of in vitro passages of virus. virus was not recovered from chickens inoculated with hvt highly passaged in vitro, which had become a high producer of cell-free virus in vitro ... | 1979 | 231437 |
| increased agglutinability and haemadsorption of marek's disease hprs line 1 lymphoblastoic cells after concanavalin a treatment. | the hprs line 1 lymphoblastoid cells derived from an ovarian lymphoma induced by marek's disease virus in chickens displayed increased agglutinability and haemadsorption when incubated with con a. the increase was proportional to the concentration of con a used. the same con a-untreated cells and normal chicken lymphocytes incubated with con a exhibited no increase in agglutinability and haemadsorption, however. | 1979 | 231521 |
| chronological study of ultrastructural changes in the peripheral nerves in marek's disease. | a chronological study was made of the ultrastructural changes in peripheral nerves following inoculation of 1-day-old chicks with a neurogenic strain of marek disease virus. no virus particles were found in nerves. cellular infiltration of nerves was detected as early as 5 days after inoculation and by 3 weeks some nerves contained proliferative lesions which possessed many of the ultrastructural features characteristic of normal, reactive lymphoid tissue. about 4 weeks after inoculation, coinci ... | 1979 | 231745 |
| influence of rabies virus on the oncogenesis in marek's disease. | | 1979 | 232370 |
| marek's disease tumor-associated surface antigen (matsa) in resistant versus susceptible chickens. | the appearance of matsa-bearing cells in the spleens of genetically susceptible (p-line) and resistant (n-line) chickens followed similar patterns at 4 and 6 days postinoculation (pi), with 4 to 10% of the cells positive in indirect fluorescent-antibody tests. levels of matsa-positive cells at 10, 14, and 21 days pi continued at 6-8% in p-line birds but dropped from about 8% to below 1 or 2% in n-line birds. this pattern was seen also in virus-isolation rates from the same spleen samples. viral ... | 1979 | 232656 |
| loss of cell-associated marek's disease vaccine titer during thawing, reconstitution, and use. | tests of the effects of mismanagement practices observed in hatcheries showed that very subtle deviations from the explicit directions accompanying marek's vaccine can cause dramatic losses of vaccine potency. such mismanagement was found to result in 14 to 97% loss of vaccine titer. it appears that procedures used in hatcheries for the handling of cell-associated marek's vaccine should be closely monitored on a continuous basis to prevent a gradual drift from correct handling. | 1979 | 232657 |
| pathogenesis of marek's disease; effect of immunization with inactivated viral and tumor-associated antigens. | inactivated marek's disease virus-infected chicken kidney cells and inactivated msb-1 lymphoblastoid marek's disease tumor cells were used to immunize chickens as virus- and tumor-associated antigens, respectively. immune and nonimmune birds were then challenged by exposure to live virulent marek's disease virus. both vaccines protected significant numbers of chickens (p less than 0.05) against subsequent tumor development, although viral antigen appeared superior to tumor antigen. after challen ... | 1979 | 232690 |
| marek's disease in comparative medicine. | | 1979 | 233254 |