| identification of cadmium-sensitive genes in the antarctic fish chionodraco hamatus by messenger rna differential display. | to investigate the ability of cadmium to affect gene transcription in fish, the messenger rna (mrna) differential display technique was used to analyze gene expression in the antarctic icefish chionodraco hamatus exposed to sublethal doses of cadmium salt. seven dna complementary to rna (cdna) bands whose steady-state levels of expression significantly changed in response to cadmium exposure were identified. the results obtained show that two groups of genes are affected by cadmium in icefish li ... | 2002 | 12459259 |
| identification of a fasl-like molecule in leucocytes of the teleost fish gilthead seabream (sparus aurata l.). | the possible expression of fasl in gilthead seabream leucocytes was studied by flow cytometry, immunoblotting and immunocytochemistry, using an anti-mouse fasl monoclonal antibody. the results pointed to a cytosolic fasl-like, but not a membrane-like form, in resting leucocytes from head-kidney, thymus, spleen, blood and peritoneal exudate. immunoblotting revealed a 19kda band in resting leucocytes, while activated leucocytes showed the same band and another of 39kda. the fasl-like molecule is i ... | 2003 | 12477498 |
| localization of igf-i, igf-i receptor, and igfbp-2 in developing umbrina cirrosa (pisces: osteichthyes). | in this study, the distribution of igf-i, igf type i receptor (igf-ir), and igf-binding protein 2 (igfbp-2) was investigated during larval and post-larval developmental stages of the shi drum (umbrina cirrosa) by immunohistochemistry using antisera raised against sparus aurata igf-i and igf-ir, and against mouse igfbp-2. immunoreactivity of the mitogenic marker pcna (proliferating cell nuclear antigen) was used for assessment of cellular proliferation. distribution of igf-i mrna was studied by i ... | 2003 | 12606266 |
| matrix gla protein gene expression and protein accumulation colocalize with cartilage distribution during development of the teleost fish sparus aurata. | matrix gla protein (mgp) is a member of the family of extracellular mineral-binding gla proteins, expressed in several tissues with high accumulation in bone and cartilage. although the precise molecular mechanism of action of this protein remains unknown, all available evidence indicates that mgp plays a role as an inhibitor of mineralization. we investigated the sites of gene expression and protein accumulation of mgp throughout development of the bony fish sparus aurata, by in situ hybridizat ... | 2003 | 12667547 |
| phagocytosis and peroxidase release by seabream (sparus aurata l.) leucocytes in response to yeast cells. | a flow cytometric method was adapted to evaluate phagocytosis by gilthead seabream leucocytes after incubation with yeast cells (saccharomyces cerevisiae). head-kidney leucocytes were incubated in vitro for different times in different proportions with heat-killed fluorescein isothiocyanate (fitc)-labeled yeast cells to study the kinetics of phagocytosis. attached and internalized yeast cells were differentiated by quenching fitc-labeled s. cerevisiae with trypan blue dye. only internalized cell ... | 2003 | 12704699 |
| a mannose-receptor is possibly involved in the phagocytosis of saccharomyces cerevisiae by seabream (sparus aurata l.) leucocytes. | in this paper the possible involvement of the mannose-receptor on the non-specific recognition and phagocytosis of heat killed yeast cells (saccharomyces cerevisiae) by gilthead seabream (sparus aurata l.) head-kidney leucocytes was established by studying the ability of different sugars to inhibit the uptake of the yeast cells by leucocytes. leucocytes were preincubated for 30min with different concentrations of sugar (alpha-mannan, d-mannose, d-fucose, l-fucose, d-glucose, d-glucosamine and n- ... | 2003 | 12711272 |
| pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream sparus aurata. | the present study investigated the kinetic profile of flumequine (flu) in gilthead sea bream sparus aurata (170 g) held at 19 degrees c and evaluated its in vitro efficacy against important bacterial diseases in mediterranean mariculture. following a single intravascular injection (10 mg kg(-1) fish), the distribution half-life (t1/2alpha) and the half-life of the terminal phase of elimination (t1/2gamma) of the drug were 0.2 and 30 h respectively. tissue penetration of flu was low, since both t ... | 2003 | 12718468 |
| carriage of potentially fish-pathogenic bacteria in sparus aurata cultured in mediterranean fish farms. | a bacteriological survey of gilthead sea bream sparus aurata from different fish farms and culture systems on the spanish mediterranean coast was conducted. three different studies were performed. study a included hatchery-reared larvae; study b, periodic examination of randomly sampled growing fish; and study c, growing fish sampled only during mortality/morbidity events. in studies b and c, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical sign ... | 2003 | 12747637 |
| existence of two o-serotypes in the fish pathogen pseudomonas anguilliseptica. | the serological characteristics of a group of 32 pseudomonas anguilliseptica strains isolated in spain from seabream (sparus aurata) and turbot (scophthalmus maximus) were compared with a total of 18 collection strains of this bacterial species with different geographical and host origin. the employment of different techniques, including slide agglutination, microagglutination and dot blot, allowed us to establish two serological groups, one comprising practically all the eel isolates, and the o ... | 2003 | 12829386 |
| virulence and molecular typing of vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and european sea bass. | vibrio harveyi was isolated from internal organs or ulcers of diseased and apparently healthy gilthead sea bream (sparus aurata) and european sea bass (dicentrarchus labrax) cultured in several fish farms located on the spanish mediterranean coast. the prevalence of the bacterium was significantly higher in european sea bass than in gilthead sea bream, and was closely related to the season in both fish species, occurring almost exclusively on warm months (june to november). after phenotypic char ... | 2003 | 12866856 |
| determination of tissue and plasma concentrations of pthrp in fish: development and validation of a radioimmunoassay using a teleost 1-34 n-terminal peptide. | a specific and sensitive radioimmunoassay (ria) for the n-terminus of sea bream (sparus auratus) and flounder (platichthys flesus) parathyroid hormone-related protein (pthrp) was developed. a (1-34) amino-terminal sequence of flounder pthrp was synthesized commercially and used as the antigen to generate specific antiserum. the same sequence with an added tyrosine (1-35(tyr)) was used for iodination. human (1-34) parathyroid hormone (pth), human (1-34) pthrp, and rat (1-34) pthrp did not cross-r ... | 2003 | 12899855 |
| assessment of dna vaccine potential for gilthead sea bream (sparus aurata) by intramuscular injection of a reporter gene. | naked circular plasmid dna containing the cytomegalovirus (cmv)-promoter-driven lacz reporter gene (pcmv-lacz) was injected in the epaxial muscle of gilthead sea bream (sparus aurata). a mosaic pattern of expression of beta-galactosidase (beta-gal) in the myofibres at the site of injection was visualised by in situ histochemical staining using 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside. as measured by o-nitrophenyl-beta-d-galactopyranoside assay, beta-gal enzymatic activity was found to ... | 2003 | 12969650 |
| fsh-, lh-, and tsh-expressing cells during development of sparus aurata l. (teleostei). an immunocytochemical study. | follicle-stimulating hormone-like gonadotropin (fsh), luteinizing hormone-like gonadotropin (lh), and thyrotropin (tsh) cells were detected in adult and developing pituitary gland of gilthead seabream. antisera obtained against the alpha- and beta-subunits of fsh (anti-my fsh) and the beta-subunit of lh (anti-my lhbeta), respectively, of the teleost mediterranean yellowtail, and an antiserum against the beta-subunit of human tsh (anti-h tshbeta), were applied to identify and follow these cells d ... | 2003 | 13129505 |
| distribution and potential role of cytosolic water-soluble phosphodiesters in fish. | the distribution of water-soluble phosphodiesters (wspdes) visible by nuclear magnetic resonance (nmr) in some intact tissues of rainbow trout (oncorhynchus mykiss walbaum) and in perchloric extracts after partial purification was examined by (31)p nmr spectroscopy. the compounds of interest were serine ethanolamine phosphate (sep), threonine ethanolamine phosphate (tep), glycerophosphorylcholine (gpc), and glycerophosphorylethanolamine (gpe). tep and sep were mostly accumulated in the heart and ... | 2003 | 13130434 |
| immunostimulant properties of a cell wall-modified whole saccharomyces cerevisiae strain administered by diet to seabream (sparus aurata l.). | the possible differences in the immunostimulant effects between whole wild saccharomyces cerevisiae and a mutant strain (fks-1) administered in the diet to gilthead seabream (sparus aurata l.) were studied. fish were fed diets containing 0 (control) or 10 g whole wild yeast or fks-1 strain per kilogram feed for 2, 4 or 6 weeks. after each sampling, the innate humoral (complement, lysozyme and peroxidases) and cellular (intracellular peroxidases, phagocytosis, respiratory burst activity and cytot ... | 2003 | 14592731 |
| production and mechanism of secretion of interleukin-1beta from the marine fish gilthead seabream. | mammalian interleukin-1beta (il-1beta) is a secretory cytokine lacking a signal peptide, which does not follow the classical endoplasmic reticulum to golgi pathway of secretion. its post-translational processing by il-1beta-converting enzyme (ice) and subsequent release from activated macrophages requires atp acting on p2x7 receptors. little information is available on the production and release of fish il-1beta, but the il-1beta gene sequences reported to date lack a conserved ice recognition s ... | 2004 | 14642889 |
| immune response of gilt-head seabream (sparus aurata) to antigens from photobacterium damselae subsp. piscicida. | | 2004 | 14675834 |
| intraspecific diversity of the marine fish pathogen tenacibaculum maritimum as determined by randomly amplified polymorphic dna-pcr. | the aim of the present study was to evaluate the intraspecific genetic variability within tenacibaculum maritimum strains isolated from different species of marine fish. | 2004 | 15012827 |
| acidophilic granulocytes of the marine fish gilthead seabream (sparus aurata l.) produce interleukin-1beta following infection with vibrio anguillarum. | the fish immune response to gram-negative bacteria is poorly understood. in this study, we use a monoclonal antibody (mab) specific to acidophilic granulocytes from the marine fish gilthead seabream (sparus aurata l.), together with an antiserum specific to interleukin-1beta (il-1beta) from this species, in order to investigate whether these cells are involved in the immune response against the pathogenic bacterium vibrio anguillarum and, in particular, in the production of the pro-inflammatory ... | 2004 | 15045578 |
| high resolution crystal structures of piscine transthyretin reveal different binding modes for triiodothyronine and thyroxine. | transthyretin (ttr) is an extracellular transport protein involved in the distribution of thyroid hormones and vitamin a. so far, ttr has only been found in vertebrates, of which piscine ttr displays the lowest sequence identity with human ttr (47%). human and piscine ttr bind both thyroid hormones 3,5,3'-triiodo-l-thyronine (t(3)) and 3,5,3',5'-tetraiodo-l-thyronine (thyroxine, t(4)). human ttr has higher affinity for t(4) than t(3), whereas the reverse holds for piscine ttr. x-ray structures o ... | 2004 | 15082720 |
| effects of high doses of cortisol on innate cellular immune response of seabream (sparus aurata l.). | the effects of high doses of cortisol, biologically the most active corticosteroid in the circulating blood of teleost fish, on gilthead seabream (sparus aurata l.) leucocytes were determined. leucocytes isolated from the head-kidney (the principal haemopoietic organ of this fish species) were incubated with five different concentrations (ranging from 0 to 10(-1) mm) of cortisol for 30, 120, 240, 360, and 480 min and their effects on leucocyte viability and some of the main innate cellular immun ... | 2004 | 15094339 |
| activation of the nitric oxide response in gilthead seabream after experimental infection with photobacterium damselae subsp. piscicida. | inoculation of small gilthead seabream (sparus aurata) (30-75 g body weight) with a sublethal dose of different photobacterium damselae subsp. piscicida (pdp) strains (di-21 and 94/99) induced an increase in serum concentrations of stable nitric oxide (no) metabolites lasting from 6 h to six days post-infection, with a peak at 24 h. in contrast, no such response was detected in larger fish (150-600 g). since the virulence of pdp correlates with the presence of a polysaccharide capsular layer whi ... | 2004 | 15110332 |
| glucan receptor but not mannose receptor is involved in the phagocytosis of saccharomyces cerevisiae by seabream (sparus aurata l.) blood leucocytes. | | 2004 | 15123311 |
| the effect of dietary administration of the fungus mucor circinelloides on non-specific immune responses of gilthead seabream. | the immunostimulant potential of the whole fungus mucor circinelloides administered in the diet to gilthead seabream (sparus aurata l.) was studied. three different lyophilised strains were used: the wild-type (r7b) and two mutant strains in the carotene-production pathway which are enriched in lycopene (mu224) or beta-carotene (t31). fish were fed diets containing 0 (control) or 10 g of one of the whole m. circinelloides strains per kg feed. after 2, 4 or 6 weeks of treatment the growth rate as ... | 2004 | 15123327 |
| developmental expression of three forms of gonadotropin-releasing hormone and ontogeny of the hypothalamic-pituitary-gonadal axis in gilthead seabream (sparus aurata). | to address the complexity of the origin of the gnrh system in perciforms, we investigated the ontogenic expression of three gnrhs in gilthead seabream. using in situ hybridization, chicken (c) gnrh-ii mrna-expressing cells were detected in the hindbrain at 1.5 days postfertilization (dpf) and in the midbrain at 2 dpf and thereafter; the hindbrain signals became undetectable after 10 dpf. salmon (s) gnrh mrna-expressing cells were first seen in the olfactory placode at 3 dpf, started caudal migra ... | 2004 | 15163612 |
| the tumor necrosis factor alpha of the bony fish seabream exhibits the in vivo proinflammatory and proliferative activities of its mammalian counterparts, yet it functions in a species-specific manner. | information on the bioactivities of non-mammalian cytokines is scant due to the lack of the recombinant molecules and specific antibodies. we produced the mature predicted peptide of tumor necrosis factor alpha (tnf alpha) from the bony fish gilthead seabream (sparus aurata l.) (sbtnf alpha), and its biological role was determined in vitro and in vivo. we first demonstrated by analytical size-exclusion chromatography that sbtnf alpha is an oligomeric protein but the dimer appears to predominate ... | 2004 | 15170511 |
| protein and glycoprotein content of lymphocystis disease virus (lcdv). | the polypeptide and glycoprotein composition of eight strains of the fish-pathogenic lymphocystis disease virus (lcdv) isolated from gilt-head seabream (sparus aurata), blackspot seabream (pagellus bogaraveo), and sole (solea senegalensis) were determined. the protein electrophoretic patterns of all lcdv isolates were quite similar regardless of the host fish, showing two major proteins (79.9 and 55.6 kda) and a variable number of minor proteins. three groups of lcdv isolates were distinguished ... | 2004 | 15248160 |
| potential drug (oxytetracycline and oxolinic acid) pollution from mediterranean sparid fish farms. | the potential for input of two common antibacterial agents in mediterranean fish farms, oxytetracycline (otc) and oxolinic acid (oa), was estimated from measurements of these drugs in the faecal excretions of two important farmed sparids, gilthead sea bream, sparus aurata and sharpsnout sea bream diplodus puntazzo. oxolinic acid was found to be well absorbed by gilthead sea bream (92%) and sharpsnout sea bream (88%) while the absorption of otc was found to be considerably lower in both species ( ... | 2004 | 15276333 |
| nodavirus infections in israeli mariculture. | viral encephalopathy and retinopathy (ver) infections were diagnosed in five fish species: epinephelus aeneus, dicentrarchus labrax, sciaenops ocellatus, lates calcarifer and mugil cephalus cultured on both the red sea and mediterranean coasts of israel during 1998-2002. spongiform vacuolation of nervous tissue was observed in histological sections of all examined species. with transmission electron microscopy, paracrystalline arrays and pieces of membrane-associated non-enveloped virions measur ... | 2004 | 15291788 |
| maternal transfer of humoral specific and non-specific immune parameters to sea bream (sparus aurata) larvae. | immunisation of sea bream (sparus aurata l.) broodstock with a novel vaccine mixture of photobacterium damsela subsp. piscicida sk7 (phdp) was performed during the period of egg development and the changes in specific and non-specific humoral immune parameters were measured. total immunoglobulin level, specific antibody titre, anti-protease activity and lysozyme activity were significantly higher in immunised parents compared to the control. after spawning significantly higher anti-protease acti ... | 2004 | 15313509 |
| toxicity of a commercial herbicide containing terbutryn and triasulfuron to seabream (sparus aurata l.) larvae: a comparison with the microtox test. | the present study aimed to compare the sensitivity of larvae of the gilthead seabream (sparus aurata), a valuable fish species of the spanish south atlantic littoral, with the extensively used microtox test on a commercial herbicide formulation containing terbutryn (59.4%) and triasulfuron (0.6%). to this purpose, mortality displayed by endogenous feeding s. aurata larvae exposed during 72 h post-hatching to nominal concentrations of the commercial formulation and bioluminescence of the marine b ... | 2004 | 15327877 |
| total serum immunoglobulin m levels are affected by immunomodulators in seabream (sparus aurata l.). | immunoglobulin m (igm) is a major component of the teleost humoral immune system. despite the significance of igm levels as an immune parameter, there are relatively few studies on changes induced in its total levels in serum. this study examines the effects of several immunomodulators (vitamin a, chitin, yeast cells or levamisole, which act as immunostimulants, and crowding, hypoxia or anaesthetics, which act as stressors) upon the total serum igm levels of non-immunized gilthead seabream (spar ... | 2004 | 15350750 |
| characterization and comparison of fatty acyl delta6 desaturase cdnas from freshwater and marine teleost fish species. | fish are the most important dietary source of the n-3 highly unsaturated fatty acids (hufa), eicosapentaenoic (epa) and docosahexaenoic acid (dha), that have particularly important roles in human nutrition reflecting their roles in critical physiological processes. the objective of the study described here was to clone, functionally characterize and compare expressed fatty acid desaturase genes involved in the production of epa and dha in freshwater and marine teleost fish species. putative fatt ... | 2004 | 15465674 |
| effects of lactoferrin on non-specific immune responses of gilthead seabream (sparus auratus l.). | the main innate cellular immune responses of gilthead seabream (sparus auratus l.) leucocytes were evaluated after in vitro incubation with human lactoferrin (lf). isolated head-kidney leucocytes were incubated with 0 (control) to 1 mg ml(-1) lf-supplemented culture medium for 30, 120, 240 or 360 min and assayed for viability, peroxidase content, and respiratory burst, phagocytic and cytotoxic activities. only respiratory burst activity was found to increase when using the highest lf concentrati ... | 2005 | 15475308 |
| vibrio ponticus sp. nov., a neighbour of v fluvialis-v. furnissii clade, isolated from gilthead sea bream, mussels and seawater. | a new vibrio species, vibrio ponticus, is proposed to accommodate four marine bacteria isolated from sea water, mussels and diseased sea bream (sparus aurata), at the mediterranean coast of spain. strains are gram negative, slightly halophilic bacteria that require na+ ion for growth, oxidase and catalase positive, negative for arginine dihydrolase and ornithine decarboxylase but positive for lysine decarboxylase and indole, and utilize beta-hydroxybutyrate as a sole carbon source. phylogenetic ... | 2004 | 15490554 |
| comparative toxic effects of formulated simazine on vibrio fischeri and gilthead seabream (sparus aurata l.) larvae. | the use of early life stage (els) tests is a useful tool in risk assessment. the purpose of this study was to compare the sensitivity of the seabream (sparus aurata) larvae with the extensively used microtox test on a commercial formulation containing simazine, an s-triazine herbicide. to this end, survival, growth and histopathological changes displayed by seabream yolk sac larvae exposed during 72 h post-hatching to nominal concentrations of the commercial preparation up to its saturating conc ... | 2004 | 15519419 |
| genomic structure and functional analysis of promoter region of somatolactin gene of sea bream (sparus aurata). | somatolactin (sl) is a pituitary hormone belonging to the growth hormone-prolactin family and is produced in the intermediate lobe of teleosts. the sl gene was isolated from a sea bream genomic library and found to be composed of 5 exons distributed within a 9-kb length of dna. sequence analysis of the proximal promoter region showed the presence of a classical tata box located 59 bp upstream from the initial start atg codon, 5 consensus sequences corresponding to the pit-1 binding element, and ... | 2004 | 15549652 |
| production and characterization of recombinantly derived peptides and antibodies for accurate determinations of somatolactin, growth hormone and insulin-like growth factor-i in european sea bass (dicentrarchus labrax). | a specific radioimmunoassay (ria) for european sea bass (dicentrarchus labrax) growth hormone (gh) was developed and validated. for this purpose, a stable source of gh was produced by means of recombinant dna technology in a bacteria system. the identity of the purified protein (ion exchange chromatography) was demonstrated by western blot and a specific gh antiserum was raised in rabbit. in western blot and ria system, this antiserum recognized specifically native and recombinant gh, and it did ... | 2004 | 15560873 |
| evaluating the effects of protection on fish predators and sea urchins in shallow artificial rocky habitats: a case study in the northern adriatic sea. | man-made defence structures (e.g., breakwaters, jetties) are becoming common features of marine coastal landscapes all around the world. the ecology of assemblages of species associated with such artificial structures is, however, poorly known. in this study, we evaluated the density and size of fish predators of echinoids (i.e., diplodus sargus, diplodus vulgaris, sparus aurata), and the density of sea urchins (i.e., paracentrotus lividus) at defence structures (i.e., breakwaters) inside and ou ... | 2005 | 15589985 |
| transcriptional regulation of glucose-6-phosphatase catalytic subunit promoter by insulin and glucose in the carnivorous fish, sparus aurata. | increase in glucose-6-phosphatase catalytic subunit (g6pase, g6pc) transcription enhances hepatic glucose production in non-insulin-dependent diabetes mellitus (niddm). the fact that carnivorous fish is an alternative model to study niddm led us to clone and characterise the first g6pc promoter region reported for fish and non-mammalian animals. the 5'-flanking region of g6pc from gilthead sea bream (sparus aurata) was isolated by chromosome walking. with smart race-pcr, the transcription start ... | 2004 | 15591035 |
| epidemiology of cryptosporidium molnari in spanish gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.) cultures: from hatchery to market size. | a long-term epidemiological study of cryptosporidium molnari in aquacultured european sea bass (esb) and gilthead sea bream (gsb) was performed in different types of facilities on the atlantic, cantabric, and mediterranean coasts. four types of studies were carried out. in study a, fish raised from juveniles to marketable size (ongrowing stage) were periodically sampled in three different types of cultures. studies b and c focused on hatchery and nursery facilities. in study d, occasional sampli ... | 2005 | 15640180 |
| early innate immune response and redistribution of inflammatory cells in the bony fish gilthead seabream experimentally infected with vibrio anguillarum. | an obvious difference between the immune system of fish and mammals is that fish lack both bone marrow and lymph nodes; in their place, the head-kidney acts as a major haematopoietic and lymphoid organ in adult fish, whereas the thymus, spleen and mucosa-associated lymphoid tissues are common to both fish and mammals. this suggests that differences exist in antigen presentation and naive lymphocyte stimulation, a prerequisite for the initiation of adaptive immune responses. intraperitoneal injec ... | 2005 | 15714279 |
| evaluation of candidate probiotic strains for gilthead sea bream larvae (sparus aurata) using an in vivo approach. | the aim of this study was to evaluate the effect of six bacterial strains on gilthead sea bream larvae (sparus aurata). | 2005 | 15752217 |
| isolation of lymphocystis disease virus from sole, solea senegalensis kaup, and blackspot sea bream, pagellus bogaraveo (brunnich). | two viruses were isolated from cultured sole, solea senegalensis, and wild blackspot sea bream, pagellus bogaraveo, and preliminarily characterized as lymphocystis disease viruses (lcdvs). viral isolates were characterized by morphological, biochemical and biophysical properties. in addition, the susceptibility of four fish cell lines was also tested. lcdv isolates developed cytopathic effects on the saf-1 cell line at 5 and 6 days post-infection and reached titres of 10(6) tcid50 ml(-1). the an ... | 2005 | 15813864 |
| adhesion to sole, solea senegalensis kaup, mucus of microorganisms isolated from farmed fish, and their interaction with photobacterium damselae subsp. piscicida. | abstract most studies carried out to select microorganisms as candidate probiotics have focused on in vitro antagonism tests, such as the production of inhibitory compounds against pathogenic microorganisms. however, attachment to mucous surfaces could be another criterion to be considered when selecting potential probiotics for aquaculture. nineteen isolates obtained from farmed senegalese sole, solea senegalensis kaup, and gilthead sea bream, sparus aurata l., have been evaluated for their cap ... | 2005 | 15813865 |
| ontogeny of the thyroid hormones and cortisol in the gilthead sea bream, sparus aurata. | the aim of the study was to elucidate the pattern of changes in the whole body concentrations of thyroid hormones (th) and cortisol in eggs, pre-larvae, and larvae in the gilthead sea bream, sparus aurata. the ontogeny of these hormones was related to specific morphological characteristics that characterize early development. th and cortisol were detected in substantial amounts in the eggs of sea bream. t3 concentration in eggs was found to be higher than that of t4 (t3, 4.02+/-0.38 ng g-1; t4, ... | 2005 | 15862562 |
| the effect of sea bream (sparus aurata) broodstock and larval vaccination on the susceptibility by photobacterium damsela subsp. piscicida and on the humoral immune parameters. | sea bream broodstock were immunised 1 or 2 months before spawning with a novel photobacteriosis vaccine. sixty-seven-day-old larvae (mean weight 22.3 mg) originating from immunised and non-immunised parents were experimentally infected with the photobacterium damsela subsp. piscicida (phdp). larvae from immunised fish showed delayed onset and lower mortality (66.67%) compared with larvae from control fish (80%). eighty-nine-day-old larvae (mean weight 162.2 mg) from both groups were bath vaccina ... | 2005 | 15863015 |
| measurement of pthrp, pthr1, and casr expression levels in tissues of sea bream (sparus aurata) using quantitative pcr. | a quantitative pcr (q-pcr) method has been established to measure the mrna expression levels of parathyroid hormone-related protein (pthrp), parathyroid hormone receptor type 1 (pthr1), and calcium-sensing receptor (casr) in sea bream (sparus aurata), using the housekeeping gene, beta-actin, as endogenous control. taqman primers and probes were designed using the primer express program, according to the published/unpublished sequences of the three target genes and beta-actin of sea bream. differ ... | 2005 | 15891056 |
| bacterial vaccines for fish--an update of the current situation worldwide. | during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ... | 2005 | 15962470 |
| vaccination strategies to prevent emerging diseases for spanish aquaculture. | in recent years, three serious diseases have emerged in spanish aquaculture. these are lactococcosis caused by lactococcus garvieae, which is of economical importance in rainbow trout (oncorhynchus mykiss); pseudomonadiasis caused by pseudomonas anguilliseptica which affects gilthead seabream (sparus aurata) and turbot (scophthalmus maximus); and flexibacteriosis caused by tenacibaculum maritimum which became a devastating problem in the emerging culture of sole (solea spp). to obtain useful inf ... | 2005 | 15962472 |
| n6-cyclohexyl-adenosine but not adenosine is a modulator of teleost fish innate immune activities. | the possible immuneregulatory effects of two purine nucleotides, adenosine and cyclohexyladenosine, on gilthead seabream (sparus aurata l.) leucocytes were investigated. leucocytes isolated from the head-kidney were incubated with five different concentrations (ranging from 0 to 100 microm) of adenosine or cyclohexyladenosine for 30, 180 and 360 min and the effect on leucocyte viability and some of the main innate cellular immune responses was evaluated. adenosine did not significantly affect th ... | 2006 | 15975652 |
| production and characterisation of gilthead sea bream (sparus auratus) recombinant parathyroid hormone related protein. | the production and purification of gilthead sea bream recombinant parathyroid hormone related protein [sbpthrp(1-125)] using an escherichia coli system and one step purification process with continuous elution gel electrophoresis is reported. the cdna encoding sbpthrp(1-125) was cloned into a prokaryotic expression vector pet-11a. the recombinant plasmid was used to transfect e. coli bl21(de3) plyss and sbpthrp(1-125) synthesis was induced by addition of 1mm isopropyl-beta-d-thiogalactopyranosid ... | 2005 | 15993105 |
| immunoglobulin protein and gene transcripts in sea bream (sparus aurata l.) oocytes. | cellular mechanisms of ig transfer to sparus aurata developing oocytes were analysed. homologous serum ig was purified and used to raise the rabbit polyclonal antiserum ora. immunohistochemistry revealed an active role of both follicular cells (already at a pre-vitellogenetic stage) and oocytes in the ig uptake. early vitellogenetic oocytes (lipid vesicle stages) had ora staining of outer cortex and oolemma as well as of their follicular cells. in protein yolk granule oocytes, ora staining was n ... | 2006 | 16040254 |
| molecular cloning, tissue distribution, and ontogeny of mrna expression of growth hormone in orange-spotted grouper (epinephelus coioides). | a full-length cdna encoding for growth hormone (gh) was cloned from orange-spotted grouper (epinephelus coioides) pituitary using reverse transcription and rapid amplification of cdna ends (race). the gh precursor cdna consists of 956 bp in size with a 85 bp 5'-untranslated region and 259 bp 3'-untranslated region. the 612 bp open reading frame encodes a 204 amino acid (aa) protein, which represents the precursor of grouper gh composed of a 17 aa signal peptide followed by a 187 aa mature gh pol ... | 2005 | 16055124 |
| virulence factors and pathogenicity of hafnia alvei for gilthead seabream, sparus aurata l. | virulence factors (eae gene, haemolytic capacity, fimbriae, resistance to the bactericidal effect of serum, siderophore production) and pathogenicity for gilthead seabream, sparus aurata l., were analysed for 23 hafnia alvei strains. none of the strains used in ld50 studies were lethal for seabream at doses as high as >10(8) cfu ml(-1). in chronic challenge studies differences in severity of the inflammatory response were observed between strains. on the basis of correlation of the inflammatory ... | 2005 | 16083446 |
| molecular cloning and expression analysis of interferon regulatory factor-1 (irf-1) of turbot and sea bream. | the interferon regulatory factor (irf) family comprises transcription factors that regulate the expression of interferon and interferon-related cytokines. using the race technique, we have determined the complete cdna sequence of turbot (scophthalmus maximus) and sea bream (sparus aurata) irfs. these sequences shared characteristics with other irfs of fish, mammals and birds, and showed high similarity with irf-1. indeed, they were included in the irf-1 cluster of the phylogenetic tree construct ... | 2006 | 16112733 |
| molecular characterization of gilthead sea bream (sparus aurata) lipoprotein lipase. transcriptional regulation by season and nutritional condition in skeletal muscle and fat storage tissues. | lipoprotein lipase (lpl) of gilthead sea bream (sparus aurata) was cloned and sequenced using a rt-pcr approach completed by 3' and 5'race assays. the nucleotide sequence covered 1669 bp with an open reading frame of 525 amino acids, including a putative signal peptide of 23 amino acids long. sequence alignment and phylogenetic analysis revealed a high degree of conservation among most fish and higher vertebrates, retaining the consensus sequence the polypeptide "lid", the catalytic triad and ei ... | 2005 | 16115788 |
| time course variations of antioxidant enzyme activities and histopathology of gilthead seabream gills exposed to malathion. | in a widely distributed and commercially important fish, gilthead seabream sparus aurata l., we have studied sublethal effects of malathion in order to identify early warning bioindicators of exposure before irreversible damage occurs. to achieve this goal, groups of 10 juvenile specimens were exposed for 24, 48, 72 and 96 h to a sublethal concentration of malathion (0.4 mg/l). another group was used as control. the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione ... | 2005 | 16136482 |
| cloning, distribution and up-regulation of the teleost fish mhc class ii alpha suggests a role for granulocytes as antigen-presenting cells. | the major histocompatibility complex (mhc) class ii alpha chain gene of the teleost fish gilthead seabream (sparus aurata), spau-daa, has been characterized. we cloned, sequenced and studied its polymorphism, before evaluating its expression in resting seabream leucocytes, tissues and tumor cells as well as in primed leucocytes. a complete allele was obtained by overlapping sequence fragments obtained by rt-pcr. the full-length spau-daa*101 comprises 1840 bp with a 5'-utr region of 84 bp, an orf ... | 2006 | 16168483 |
| interactions of microorganisms isolated from gilthead sea bream, sparus aurata l., on vibrio harveyi, a pathogen of farmed senegalese sole, solea senegalensis (kaup). | four bacterial isolates from farmed gilthead sea bream, sparus aurata, included in a previous study as members of the vibrionaceae and pseudomonodaceae and the genus micrococcus, have been evaluated for their adhesive ability to skin and intestinal mucus of farmed senegalese sole, solea senegalensis, and their antagonistic effect on vibrio harveyi, a pathogen of sole. these isolates showed higher adhesion to sole mucus than the pathogenic strains of v. harveyi assayed. only two of the isolates s ... | 2005 | 16266326 |
| life-history- and ecosystem-driven variation in composition and residence pattern of seabream species (perciformes: sparidae) in two mediterranean coastal lagoons. | species composition and length-frequency distributions of six sparid fish species were investigated in two central mediterranean coastal lagoons off the western coast of italy: fogliano and caprolace. in the former, the sparid fauna was dominated by the gilt-head seabream (sparus aurata), whereas in caprolace, species composition was more homogeneous across all six species. size structure varied considerably among species: s. aurata, diplodus puntazzo and diplodus vulgaris had a single-cohort st ... | 2006 | 16266729 |
| detection of lymphocystis disease virus (lcdv) in asymptomatic cultured gilt-head seabream (sparus aurata, l.) using an immunoblot technique. | an immunoblot technique for the detection of lymphocystis disease virus (lcdv) in naturally infected gilt-head seabream (sparus aurata, l.) has been developed. a specific antiserum against a 60 kda viral protein has been proven to be an appropriate tool for lcdv diagnosis either from inoculated cell cultures or from fish tissues using the immunoblot assay. the sensitivity of this technique varied between 10(-1) and 10(2) tcid50. lcdv has also been detected in fish tissues from both, diseased and ... | 2006 | 16298500 |
| gilthead seabream ( sparus aurata l.) innate defence against the parasite enteromyxum leei (myxozoa). | the humoral innate immune response of gilthead seabream (sparus aurata l.) against the myxozoan enteromyxum leei has been studied. at 10, 22, 38, 52 and 108 days of cohabitation fish were sampled to examine gut histology and to determine serum innate immune parameters and the mrna expression of pro-inflammatory cytokines (il-1beta and tnfalpha) in head-kidney. the parasite was successfully transmitted to 45% of the recipient fish and prevalence reached a maximum (62.5%) at the last sampling time ... | 2006 | 16393358 |
| kudoa iwatai (myxosporea: multivalvulida) in wild and cultured fish in the red sea: redescription and molecular phylogeny. | gilt-head sea bream, sparus aurata l., the mediterranean's most important mariculture species, has been cultured for the last 30 yr in eilat (israeli red sea). kudoa sp. was the first myxosporean parasite reported from this species. in recent years, an increase in prevalence in both land-based and sea-cage facilities in eilat has been observed. infections with the same kudoa species appeared in cultured european sea bass dicentrarchus labrax (l.) and grey mullet mugil cephalus in the same farms, ... | 2005 | 16419765 |
| effect of heat-inactivated fish and non-fish derived probiotics on the innate immune parameters of a teleost fish (sparus aurata l.). | the in vitro effects of four heat-inactivated bacterial species on the cellular innate immune responses of the gilthead seabream (sparus aurata l.) were investigated. head-kidney leucocytes were isolated and incubated for 30 min with two bacteria isolated from seabream skin (pdp11 and 51m6; members of the vibrionaceae and in the genus shewanella) and two bacteria used as probiotics in humans and cattle (lactobacillus delbrüeckii subsp. lactis and bacillus subtilis) at 5 x 10(5), 5 x 10(6) or 5 x ... | 2006 | 16516307 |
| sterol regulatory element binding protein-1a transactivates 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene promoter. | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (pfkfb) catalyzes the synthesis and degradation of fructose-2,6-bisphosphate, a key modulator of glycolysis-gluconeogenesis. to gain insight into the molecular mechanism behind hormonal and nutritional regulation of pfkfb expression, we have cloned and characterized the proximal promoter region of the liver isoform of pfkfb (pfkfb1) from gilthead sea bream (sparus aurata). transient transfection of hepg2 cells with deleted gene promoter constr ... | 2006 | 16614080 |
| effects of administration of probiotic strains on galt of larval gilthead seabream: immunohistochemical and ultrastructural studies. | two bacterial strains lactobacillus fructivorans (as17b), isolated from adult seabream (sparus aurata l.) gut, and lactobacillus plantarum (906), isolated from human faeces, were administered contemporaneously during seabream development using brachionus plicatilis and/or artemia salina and dry feed as vectors. experimental group a received the probiotic strains already via rotifers from day 5 post-hatch (ph), whereas treatment of group b began with artemia feeding from day 27 ph. fish were samp ... | 2007 | 16730458 |
| full-length sequence and expression analysis of toll-like receptor 9 in the gilthead seabream (sparus aurata l.). | toll-like receptors (tlrs) have recently emerged as key sensors of invading microbes, acting through recognition of pathogen-associated molecular patterns. it has been demonstrated that tlr9 is involved in the recognition of unmethylated cpg motifs in mice, humans, and pigs. we report here the full-length sequence of tlr9 cdna in the gilthead sea bream (sparus aurata l.). the predicted protein (1063 amino acids) was similar to mammalian tlr9s, showing 21 leucine-rich repeats in the extracellular ... | 2006 | 16797882 |
| early local and systemic innate immune responses in the teleost gilthead seabream after intraperitoneal injection of whole yeast cells. | the early cellular innate immune responses of the teleost gilthead seabream (sparus aurata l.) against whole yeast cells were studied. fish received a single intraperitoneal (i.p.) injection of saccharomyces cerevisiae and leukocyte mobilization, degranulation, peroxidase content, respiratory burst, phagocytic and cytotoxic activities were assayed in both head-kidney leukocytes (hkls) and peritoneal exudate leukocytes (pels). the total number of pels significantly increased from 4 h post-injecti ... | 2007 | 16843011 |
| virulence and enterobacterial repetitive intergenic consensus pcr of vibrio alginolyticus strains isolated from tunisian cultured gilthead sea bream and sea bass outbreaks. | vibrio alginolyticus was isolated from the internal organs of diseased gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax) cultured in two fish farms located on the tunisian mediterranean coast, from 2003 to 2005. after phenotypic characterisation, a selection of 34 isolates from gilthead sea bream and sea bass were molecularly typed by repetitive intergenic consensus pcr (eric-pcr) showing a high polymorphism among the isolated strains (19 genotypes). most of the isolates wer ... | 2006 | 16870360 |
| parasitic diseases of marine fish: epidemiological and sanitary considerations. | over recent decades, parasitic diseases have been increasingly considered a sanitary and economic threat to mediterranean aquaculture. in order to monitor the distribution of parasites in cultured marine fish from italy and study their pathogenic effects on the host, a three-year survey based on parasitological and histopathological exams was carried out on 2141 subjects from eleven fish species and coming from different farming systems (extensive, intensive inland farms, inshore floating cages, ... | 2006 | 16881387 |
| bioaccumulation of some heavy metals in different tissues of dicentrarchus labrax l, 1758, sparus aurata l, 1758 and mugil cephalus l, 1758 from the camlik lagoon of the eastern coast of mediterranean (turkey). | in this study, bioaccumulation of the heavy metals (fe, zn, cd) in the liver, gill, gonad and muscle tissues of dicentrarchus labrax, mugil cephalus and sparus aurata were investigated seasonally. camlik is the important fisheries lagoon located in the iskenderun bay, mediterranean coast of turkey. due to heavy industrial and agricultural activities in the region, the bay has the polluted coastal waters of turkey. therefore, the levels of cadmium, zinc, iron were determined in the muscle in each ... | 2006 | 16897534 |
| interactions between bacteria and cryptosporidium molnari in gilthead sea bream (sparus aurata) under farm and laboratory conditions. | the possible interaction of cryptosporidium molnari and bacteria in gilthead sea bream (sparus aurata) was studied. epidemiological data from a pathological survey under farm conditions were analyzed. in addition, parasite and bacteria burdens were studied in experimental models in which naturally and experimentally parasitized fish were challenged with a particular strain of vibrio harveyi (h57). all the bacteria species present were studied. under farm conditions, the parasite was more prevale ... | 2006 | 16934406 |
| vanadium distribution, lipid peroxidation and oxidative stress markers upon decavanadate in vivo administration. | the contribution of decameric vanadate species to vanadate toxic effects in cardiac muscle was studied following an intravenous administration of a decavanadate solution (1mm total vanadium) in sparus aurata. although decameric vanadate is unstable in the assay medium, it decomposes with a half-life time of 16 allowing studying its effects not only in vitro but also in vivo. after 1, 6 and 12h upon decavanadate administration the increase of vanadium in blood plasma, red blood cells and in cardi ... | 2007 | 17030392 |
| isolation and characterization of a fish f-type lectin from gilt head bream (sparus aurata) serum. | a novel fucose-binding lectin, designated saufbp32, was purified by affinity chromatography on fucose-agarose, from the serum of the gilt head bream sparus aurata. electrophoretic mobility of the subunit revealed apparent molecular weights of 35 and 30 kda under reducing and non-reducing conditions, respectively. size exclusion analysis suggests that the native lectin is a monomer under the selected experimental conditions. agglutinating activity towards rabbit erythrocytes was not significantly ... | 2007 | 17095163 |
| cell-mediated cytotoxicity is the main innate immune mechanism involved in the cellular defence of gilthead seabream (teleostei: sparidae) against enteromyxum leei (myxozoa). | the cellular innate immune response of gilthead seabream (sparus aurata l.) against the myxozoan enteromyxum leei was studied. enteromyxosis was transmitted by maintaining uninfected fish (recipients) together with infected animals. a group of fish not exposed to the infection served as controls. after 10, 22, 38, 52 and 108 days, control and recipient fish were sampled and leucocyte subpopulations and cellular immune responses (leucocyte peroxidases, phagocytosis, respiratory burst and cytotoxi ... | 2006 | 17096645 |
| experimental transmission of enteromyxum leei to freshwater fish. | the myxosporean enteromyxum leei is known to infect a wide range of marine fish hosts. the objective of the present study was to determine whether freshwater fish species are also receptive hosts to this parasite. seventeen species of freshwater fish were experimentally fed e. leei-infected gut tissue from donor gilthead sea bream sparus aurata obtained from a commercial sea bream cage farm. four of the tested species, tiger barb puntius tetrazona, zebra danio danio rerio, oscar astronotus ocell ... | 2006 | 17140140 |
| kinetic of biomarker responses in juveniles of the fish sparus aurata exposed to contaminated sediments. | sediments in the national park of the atlantic islands (galicia, spain) were affected by the spill of the tanker prestige (november, 2002) and still present high levels of polycyclic aromatic hydrocarbons. the adverse effects associated with the contaminants in sediments were tested using a chronic bioassay, exposing juveniles of the fish sparus aurata (seabream). a toxicokinetic approach is proposed to evaluate sediment quality by linking chemical and ecotoxicological data along the time. sedim ... | 2007 | 17171271 |
| transcriptional regulation of arylalkylamine-n-acetyltransferase-2 gene in the pineal gland of the gilthead seabream. | pineal serotonin-n-acetyltransferase (arylalkylamine-n-acetyltransferase; aanat) is considered the key enzyme in the generation of circulating melatonin rhythms; the rate of melatonin production is determined by aanat activity. in all the examined species, aanat activity is regulated at the post-translational level and, to a variable degree, also at the transcriptional level. here, the transcriptional regulation of pineal aanat (aanat2) of the gilthead seabream (sparus aurata) was investigated. ... | 2007 | 17184485 |
| host switch of lamellodiscus elegans (monogenea: monopisthocotylea) and sparicotyle chrysophrii (monogenea: polyopisthocotylea) between cage-reared sparids. | sharpsnout bream (diplodus puntazzo) has been used in adriatic aquaculture for less than a decade, but the decreasing trend of rearing this species will probably result in its complete substitution by more exploited sea bream (sparus aurata). only two facilities still rear both fish species in neighbouring cages in monoculture. a switch of parasites was observed between sparids during monitoring of the gill monogeneans of farmed fish. in wild fish of the adriatic sea, lamellodiscus elegans (mono ... | 2007 | 17187241 |
| the type ii interleukin-1 receptor (il-1rii) of the bony fish gilthead seabream sparus aurata is strongly induced after infection and tightly regulated at transcriptional and post-transcriptional levels. | interleukin-1beta (il-1beta) is the prototypic pro-inflammatory cytokine. all the biological effects of il-1beta are mediated through interaction with type 1 il-1 receptor (il-1ri), whereas another receptor, called type 2 il-1r (il-1rii), lacks an intracellular signalling domain and acts as a decoy receptor that down-regulates responses to il-1beta. although both receptors are present in bony fish, their expression and biological role in the regulation of il-1beta activity in non-mammalian verte ... | 2007 | 17234266 |
| effects of 17beta-estradiol and 4-nonylphenol on osmoregulation and hepatic enzymes in gilthead sea bream (sparus auratus). | sexually immature sparus auratus were injected intraperitoneally with coconut oil either alone (control) or containing 17beta-estradiol (e2, 10 microg/g body mass) or 4-nonyphenol (4-np, 100 and 200 microg/g body mass) and sampled 10 days later. gill and kidney na(+),k(+)-atpase activities, plasma levels of e2 and cortisol, plasma osmolites (osmolality, sodium and chloride) and metabolites (glucose, lactate, proteins and triglycerides) were examined. livers were used for measuring hepatosomatic ... | 2007 | 17251064 |
| an unidentified epi-epithelial myxosporean in the intestine of gilthead sea bream sparus aurata l. | in the course of experimental infections of gilthead sea bream sparus aurata with the myxozoan enteromyxum leei, stages of an unidentified myxozoan were observed attached to the intestinal brush border of some fish. infection levels of the parasite, which was named "epi-epithelial myxosporean" (eem) were recorded, and its structure was studied by light microscopy (lm) and electron microscopy (em). in situ hybridisation (ish) probes specific for e. leei were developed and used to differentiate be ... | 2007 | 17333277 |
| emergence of pathogenic betanodaviruses belonging to the sjnnv genogroup in farmed fish species from the iberian peninsula. | betanodaviruses are the causative agents of viral nervous necrosis (vnn) or viral encephalopathy and retinopathy (ver) in cultured marine fish. based on the rna2 gene fish nodaviruses have been traditionally classified into four different genotypes and recently a fifth genotype has been proposed. this study presents sequencing data of 24 new nodaviruses obtained from three different fish species: sea bass, dicentrarchux labrax (l.), sea bream, sparus aurata l., and senegalese sole, solea senegal ... | 2007 | 17394524 |
| effect of host factors and experimental conditions on the horizontal transmission of enteromyxum leei (myxozoa) to gilthead sea bream, sparus aurata l., and european sea bass, dicentrarchus labrax (l.). | | 2007 | 17394527 |
| authenticating production origin of gilthead sea bream (sparus aurata) by chemical and isotopic fingerprinting. | recent eu legislation (ec/2065/2001) requires that fish products, of wild and farmed origin, must provide consumer information that describes geographical origin and production method. the aim of the present study was to establish methods that could reliably differentiate between wild and farmed european gilthead sea bream (sparus aurata). the methods that were chosen were based on chemical and stable isotopic analysis of the readily accessible lipid fraction. this study examined fatty acid prof ... | 2007 | 17464521 |
| four parasitic crustacean species from marine fishes of turkey. | the aim of this work was to present a preliminary knowledge of the parasitic copepods of marine fish of turkey. in this study, four parasitic crustaceans were reported from five different fish species found in turkish seas: lepeophtheirus europaensis (zeddam, berrebi, renaud, raibaut & gabrion, 1988) was found on the gills of the european flounder, platichtys flesus (linnaeus, 1758 (pleuronectidae); nerocila bivittata (risso, 1816) on caudal peduncles of east atlantic peacock wrasse, symphodus t ... | 2007 | 17471420 |
| survival of vibrio alginolyticus in seawater and retention of virulence of its starved cells. | the aim of this study was to evaluate the survival responses of four strains of vibrio alginolyticus in seawater under starvation conditions. we used microcosms containing sterilised seawater and incubated at ambient temperature (22-25 degrees c). v. alginolyticus maintained its culturability for at least nine months. long-term-starved cells showed an absence or a decrease in their enzymatic activities. resuscitation assays of viable but non-culturable (vbnc) cells were conducted and the recover ... | 2007 | 17524473 |
| modelling the effect of high pressure on the inactivation kinetics of a pressure-resistant strain of pediococcus damnosus in phosphate buffer and gilt-head seabream (sparus aurata). | the aim of this research was to: (i) determine the inactivation pattern of a pressure-resistant strain of pediococcus damnosus by high hydrostatic pressure in phosphate buffer (ph 6.7) and gilt-head seabream using the linear, biphasic and weibull models; and (ii) validate the applicability of the weibull model to predict survival curves at other experimental pressure levels. | 2007 | 17578414 |
| molecular and functional characterization of gilthead seabream sparus aurata caspase-1: the first identification of an inflammatory caspase in fish. | caspases are a family of cysteine proteases that fulfil critical roles in mammalian apoptosis and in the proteolytic activation of cytokines. in humans, the caspase family includes 13 members whose functions seem to correlate with their phylogenetic relationship. they are classified into two main groups, the cell death (apoptotic) and the inflammatory caspases. caspase-1 is the best characterized inflammatory caspase and is responsible for the processing of interleukin-1beta (il-1beta), il-18 an ... | 2008 | 17610954 |
| modelling metallothionein induction in the liver of sparus aurata exposed to metal-contaminated sediments. | metallothionein (mt) in the liver of gilthead seabreams (sparus aurata l., 1758) exposed to sado estuary (portugal) sediments was quantified to assess the mt induction potential as a biomarker of sediment-based contamination by copper (cu), cadmium (cd), lead (pb) and arsenic (as). sediments were collected from two control sites and four sites with different levels of contamination. sediment cu, cd, pb, as, total organic matter (tom) and fine fraction (ff) levels were determined. generalized lin ... | 2008 | 17617458 |
| characterization of the de novo biosynthetic enzyme of platelet activating factor, ddt-insensitive cholinephosphotransferase, of human mesangial cells. | platelet activating factor (paf), a potent inflammatory mediator, is implicated in several proinflammatory/inflammatory diseases such as glomerulonephritis, glomerulosclerosis, atherosclerosis, cancer, allergy, and diabetes. paf can be produced by several renal cells under appropriate stimuli and it is thought to be implicated in renal diseases. the aim of this study is the characterization of dtt-insensitive cholinephosphotransferase (paf-cpt) of human mesangial cell (hmc), the main regulatory ... | 2007 | 17710109 |
| diversity of shewanella population in fish sparus aurata harvested in the aegean sea. | to study the diversity of shewanella population in sparus aurata fish harvested in the aegean sea, as well as to elucidate the influence of fish storage conditions on the selection in shewanella strains. | 2007 | 17714405 |
| natural occurrence of metallothioneinlike proteins in liver tissues of four fish species from the northeast mediterranean sea. | fish with different ecological needs were captured from the northeast mediterranean sea to determine metallothioneinlike proteins in their livers. heat-treated liver samples were run on a column packed with sephadex g-75 (sigma, germany) for subsequent determination of metals, sulfhydryl, and absorbance at 254 and 280 nm. liver cytosols of sparus auratus and mullus barbatus had three heat-stable protein peaks, whereas mugil cephalus and atherina hepsetus had two. the third protein peak, containi ... | 2007 | 17910363 |
| phenotypic, serological and genetic characterization of pseudomonas anguilliseptica strains isolated from cod, gadus morhua l., in northern europe. | the biochemical, serological and genetic characteristics of six strains of pseudomonas anguilliseptica isolated from cod, gadus morhua, in scotland were compared to well characterized isolates of this same bacterial species but of different origin. biochemical and physiological analyses showed that this group of isolates was highly homogeneous, their characteristics matching previous descriptions of the pathogen. similar results were obtained for the six cod isolates in the serological assays, a ... | 2007 | 17958609 |
| persistence of duplicated pac1 receptors in the teleost, sparus auratus. | : duplicated genes are common in vertebrate genomes. their persistence is assumed to be either a consequence of gain of novel function (neofunctionalisation) or partitioning of the function of the ancestral molecule (sub-functionalisation). surprisingly few studies have evaluated the extent of such modifications despite the numerous duplicated receptor and ligand genes identified in vertebrate genomes to date. in order to study the importance of function in the maintenance of duplicated genes, s ... | 2007 | 17997850 |
| susceptibility of photobacterium damselae subsp. piscicida strains isolated from senegalese sole, solea senegalensis kaup, and gilthead seabream, sparus aurata l., to several antibacterial agents. | | 2008 | 18086037 |
| total substitution of fish oil by vegetable oils in gilthead sea bream (sparus aurata) diets: effects on hepatic mx expression and some immune parameters. | the use of vegetable oils in fish nutrition has been extensively studied; and recent work has focused attention on replacing fish oil with alternative fatty acid sources and their effect on the immune system. however, little is known about the effect of these oils on immune parameters such as the fish interferon system. in this study we evaluate the effect of two vegetable oils (linseed and soybean) on gilthead sea bream mx expression and other innate immune parameters. experimental diets were f ... | 2008 | 18158252 |
| differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost sparus aurata. | aquaporin (aqp)-mediated intestinal water absorption may play a major osmoregulatory role in euryhaline teleosts, although the molecular identity and anatomical distribution of aqps in the fish gastrointestinal tract is poorly known. here, we have investigated the functional properties and cellular localization in the intestine of two gilthead seabream (sparus aurata) homologs of mammalian aquaporin-1 (aqp1), named saaqp1a and saaqp1b. heterologous expression in xenopus laevis oocytes showed tha ... | 2008 | 18171690 |
| chronic exposure to the parasite enteromyxum leei (myxozoa: myxosporea) modulates the immune response and the expression of growth, redox and immune relevant genes in gilthead sea bream, sparus aurata l. | the myxosporean parasite enteromyxum leei invades the intestine of gilthead sea bream producing a slow-progressing disease, which may end in the death of fish. the present work aimed to better know the host immune response and the underlying molecular mechanisms, which may help to understand why some individuals seem to be refractory to the disease. three main aspects involved in fish health and welfare (immune, growth and redox status) were studied in fish exposed to e. leei-contaminated efflue ... | 2008 | 18343157 |