| time-course variations of dna damage and biomarkers of oxidative stress in tilapia (oreochromis niloticus) exposed to effluents from a swine industry. | dna damage (comet assay), lipoperoxidation levels (tbars), and several biomarkers of oxidative stress such as catalase (cat), glutathione reductase (gr), glutathione peroxidase (gpx), glutathione s-transferase (gst), and contents of reduced (gsh) and total (tg) glutathione were measured in liver and blood (comet assay) of tilapia (oreochromis niloticus) exposed for 7, 15, 30 (subchronic exposure), 60, and 90 days (chronic exposure) to two treatment lagoons of a swine-processing plant, the first ... | 2006 | 16247568 |
| food composition and feeding habits of some fresh water fishes in various water systems at abbassa, egypt, with special reference to snails transmitting diseases. | study of feeding habits of freshwater fishes collected from ponds at world fish center (iclarm) showed that the african catfish, clarias gariepinus and forskal catfish, bagras bayad had the highest proportion of full stomachs (31-58% & 44-45% respectively). in cichlid fishes, the rate of full stomachs was much lower, being 0.0-12.5% and showed higher incidence of empty stomachs that varied from 37.5% for oreochromis niloticus to 78.3% for sarotherodon galilaeus. food items were analyzed by the p ... | 2005 | 16083073 |
| genes encoding putative natural killer cell c-type lectin receptors in teleostean fishes. | mammalian natural killer (nk) cells are cytotoxic lymphocytes that express receptors specific for mhc class i molecules. the nk cell receptors belong to two structurally unrelated families, the killer cell ig-like receptors and the killer cell c-type lectin receptors. we describe a cdna clone derived from the bony (cichlid) fish paralabidochromis chilotes and show that it encodes a protein related to the cd94/nk cell group 2 (nkg2) subfamily of the killer cell c-type lectin receptors. the gene e ... | 2003 | 12802013 |
| expression of nitric oxide synthase in the preoptic-hypothalamo-hypophyseal system of the teleost oreochromis niloticus. | in the present study, we have analyzed the expression of nitric oxide synthase (nos) in the preoptic-hypothalamo-hypophyseal system of the teleost oreochromis niloticus. the assay for enzyme activity demonstrated that a constitutive nos activity is present both in soluble and particulate fractions of the homogenates of diencephalons. western blot analysis using an antibody against the n-terminus of human nnos revealed two bands both in the supernatant and in the pellet. one band co-migrates at a ... | 2003 | 12907859 |
| molecular cdna cloning and analysis of the organization and expression of the il-1beta gene in the nile tilapia, oreochromis niloticus. | the full-length cdna sequence of interleukin-1beta (il-1beta) from the nile tilapia, oreochromis niloticus, was determined by using pcr with primers designed from known fish il-1beta sequences followed by elongation of the 5' and 3' ends using rapid amplification of cdna ends (race). the cdna contains a 92-bp 5' untranslated region (utr), a single open reading frame (orf) of 732 bp that translates into a 243-amino acid molecule, a 341-bp 3' utr with four cytokine rna instability motifs (attta), ... | 2006 | 16451827 |
| larvivorous potential of fish species found in river bed pools below the major dams in sri lanka. | malaria in sri lanka is closely associated with the river systems in the country. construction of major dams across rivers has resulted in year-round pooling and anopheline mosquito breeding in river beds below the dams. this study was carried out to determine the larvivorous activity of fish found in river bed pools below the dams to identify the potential fish species for anopheline larval control in these areas. fish species in five river beds below the dams, namely, laxapana, kotmale, nilamb ... | 2006 | 16506451 |
| isolation, characterization and expression of 11beta-hydroxysteroid dehydrogenase type 2 cdnas from the testes of japanese eel (anguilla japonica) and nile tilapia (oreochromis niloticus). | the japanese eel (anguilla japonica) and nile tilapia (oreochromis niloticus) 11beta-hydroxysteroid dehydrogenase type 2 (11beta-hsd2) cdnas were isolated from their respective testes cdna libraries. the cdnas predict two peptides of 436 and 406 amino acid residues that share about 42% homology with mammalian 11beta-hsd type 2 proteins. analysis of the tissue distribution pattern by rt-pcr reveals that 11beta-hsd2 is expressed in a wide variety of tissues in tilapia, with higher expression in ki ... | 2003 | 14519098 |
| passive immunization of nile tilapia (oreochromis niloticus) provides significant protection against streptococcus agalactiae. | a study was conducted to determine the role of specific antibodies in immunity to streptococcus agalactiae. adult nile tilapia (oreochromis niloticus) were injected i.p. with tryptic soy broth as control or with s. agalactiae vaccine. ninety days later, fish were challenged with 1.5x10(4)cfus. agalactiae fish(-1). blood was drawn from all fish 90d after vaccination and 25d after challenge, and the acquired serum was injected i.p. in fingerling nile tilapia. these passively immunized fish were su ... | 2006 | 16531069 |
| a novel cold-adapted imidase from fish oreochromis niloticus that catalyzes hydrolysis of maleimide. | in this paper we report the first comparative study of cold-adapted imidase (ec 3.5.2.2) from the fish (oreochromis niloticus) liver and its thermophilic counterparts taken from pig liver and escherichia coli (overexpressed recombinant hydantoinase from agrobacterium radiobacter nrrl b1). approximately 6000-fold purification and a 40% yield of fish imidase activity were obtained through ammonium sulfate precipitation, octyl, chelating, deae, and hydroxyapatite chromatography. this cold-adapted i ... | 2003 | 14637160 |
| evaluation of the carcinogenic effects of furazolidone and its metabolites in two fish species. | the major metabolite from the use of furazolidone (fzd) in mammals, birds and fish is 2,3-dihydro-3-cyanomethyl-2-hydroxy-5-nitro-1alpha, 2-di(2-oxo-oxazolidin-3-yl)iminomethyl-furo[2,3-beta]furan, also called 3-amine-2-oxazolidone (aoz). a minor metabolite was identified as n-(5-amine-2-furfuryliden)-3-amine-2-oxazolidone (foz). to assess the potential carcinogenicity of fzd and the metabolic mixture of aoz/foz, 11 mg fzd/kg feed/day was fed for 12 weeks to mollies (poecilia formosa), an orname ... | 2004 | 14647403 |
| utilization of shrimp industry waste in the formulation of tilapia (oreochromis niloticus linnaeus) feed. | a rapid expansion of fisheries is demanding an adequate supply of efficient, nutritious and inexpensive fish feed, because feed contributes highly to the cost of fish production. shrimp head, a waste product from the shrimp export industry qualifies as an economical, abundant and good quality protein source for fish feeds. in the present work, shrimp head silage powder, which contained approximately 40% protein, was used as a substitute for fish flour. four feeds, in the form of pellets, were pr ... | 2007 | 16624557 |
| the biology of oreochromis niloticus in a polluted canal. | in the present investigation, length-weight relationship, condition factor, age, growth, mortality, gonadosomatic index (gsi) and fecundity of tilapias (bolti in arabic): oreochromis niloticus, from shanawan drainage canal (sdc), al-minufiya province, egypt, were studied. this was carried out on 162 fish samples collected during the period from april 1992 to may 1993. oreochromis niloticus was found to attain sexual maturity early (8-9 cm), was more fecund (1,234-3,893) and has high mortality ra ... | 2003 | 14649423 |
| induced alkoxyresorufin-o-dealkylases in tilapias (oreochromis niloticus) from guandu river, rio de janeiro, brazil. | the activity of fish monooxygenases has been extensively used as a monitoring tool to detect contamination of water bodies by cytochrome p450-inducing agents. in this study we evaluated the activities of ethoxy- (erod), methoxy- (mrod) and pentoxy- (prod) resorufin-o-dealkylases in the liver of nile tilapias (oreochromis niloticus) collected at the guandu river, at a reference clean site (lake 1) and at two other sampling sites (lakes 2 and 3) in rio de janeiro state, brazil. alkoxyresorufin-o-d ... | 2004 | 14675840 |
| clustering of c-type lectin natural killer receptor-like loci in the bony fish oreochromis niloticus. | the genome of the cichlid (teleost) fish oreochromis niloticus contains a set of genes which encode group v c-type lectin proteins homologous to the mammalian nkg2/cd94 family of natural killer (nk) cell receptors. to determine the genomic organization of these killer cell-like receptor (klr) genes, an o. niloticus bac library was screened with a cdna probe derived previously from an expressed sequence tag of the related cichlid species paralabidochromis chilotes. four distinct klr-bearing bac c ... | 2004 | 14871289 |
| genetic characterization of four strains of nile tilapia (oreochromis niloticus l.) using microsatellite markers. | four domesticated strains of nile tilapia (oreochromis niloticus l.) were genetically characterized using 14 microsatellite markers and 64 animals per strain. two strains, chitralada (ait) and international development research centers (idrc) were obtained from the ait institute, bangkok, thailand. the gift strain (5th generation) came from nagri, thailand, and the gott strain was supplied by the university of göttingen, germany. the average numbers of alleles per marker were 5.0 (gott), 5.4 (ai ... | 2004 | 15025567 |
| protective effects and mechanisms of a probiotic bacterium lactobacillus rhamnosus against experimental edwardsiella tarda infection in tilapia (oreochromis niloticus). | in recent years, probiotics, especially lactic acid bacteria, have been used as dietary supplements to protect fish from various infections. here, we examined the protective effects of lactobacillus rhamnosus against experimental edwardsiella tarda infection in tilapia (oreochromis niloticus). cumulative mortality was significantly lower in probiotic-supplemented fish than in control fish. in a histopathological survey, pyogranulomatous responses were observed at an earlier stage and to a greate ... | 2006 | 16870266 |
| ontogenic development of three gnrh systems in the brain of a pleuronectiform fish, barfin flounder. | a pleuronectiform fish, the barfin flounder verasper moseri, has three molecular forms of gonadotropin-releasing hormone (gnrh) in the brain, salmon gnrh (sgnrh), chicken gnrh-ii (cgnrh-ii) and seabream gnrh (sbgnrh). to elucidate the ontogenic origin of the neurons that produce these gnrh molecules, the development of three gnrh systems was examined by in situ hybridization and immunocytochemistry. neuronal somata that express sgnrh mrna were detected first in the vicinity of the olfactory epit ... | 2004 | 15056926 |
| evidence that tilapia islets do not express alpha-(1,3)gal: implications for islet xenotransplantation. | cell therapy for diabetes using teleost fish islet tissue has emerged as an intriguing alternative to the use of islet tissue from mammalian pancreases. the islet tissue, called brockman bodies (bbs), is anatomically distinct from the pancreatic exocrine tissue and can be easily identified and isolated. islets harvested from nile tilapia (oreochromis niloticus), when transplanted into streptozotocin-diabetic nude mice, produce long-term normoglycemia and achieve mammalian-like glucose tolerance ... | 2004 | 15099208 |
| lymphoid apoptosis in edwardsiella tarda septicemia in tilapia, oreochromis niloticus. | the present study revealed a relationship between the kinetic change of apoptosis and the inflammatory response during experimental intraperitoneal infection with edwardsiella tarda as a septicemic model. the morphological changes of apoptotic cells including cellular shrinkage, condensed nuclear chromatin, nuclear fragmentation and membrane blebbing were detected by light and transmission electron microscopy. tunel and agarose gel electrophoresis confirmed the fragmentation of dna in the apopto ... | 2007 | 17046284 |
| first report of streptococcus agalactiae and lactococcus garvieae from a wild bottlenose dolphin (tursiops truncatus). | the isolation and characterization of two bacterial species, streptococcus agalactiae and lactococcus garvieae, previously unreported in wild marine mammals are described from a freshly dead bottlenose dolphin, tursiops truncatus, from kuwait bay, kuwait, in september 2001. conventional and rapid identification systems were used to determine that isolates from muscle and kidney were s. agalactiae and l. garvieae, respectively. the isolates were gram-positive, catalase-negative, oxidase-negative, ... | 2006 | 17092887 |
| laser-captured single digoxigenin-labeled neurons of gonadotropin-releasing hormone types reveal a novel g protein-coupled receptor (gpr54) during maturation in cichlid fish. | gpr54 is a novel g protein-coupled receptor speculated to be essential for sexual development. however, its role in the regulation of gnrh types is unknown. to address this issue, we cloned gpr54 from the brain of a cichlid fish (tilapia oreochromis niloticus) and determined its expression in immature and mature males using our newly developed technique: laser-captured microdissection of single digoxigenin-labeled gnrh neurons coupled with real-time quantitative pcr. the tilapia gpr54 cdna conta ... | 2004 | 15155576 |
| reproductive aspects of oreochromis niloticus (perciformes: cichlidae) at coatetelco lake, morelos, mexico. | sex ratio, size at maturity, maturity stages, fecundity and egg diameter of oreochromis niloticus from coatetelco lake, morelos state, mexico, were studied from january to december 1993. sex ratio (male:female) was approximately 1:1.02. length at maturity was 117 mm (males) and 120 mm (females). the fecundity ranged between 104 and 709 eggs, with egg diameter from 1,000 to 3,000 microns. the gonadosomatic and hepatosomatic index indicate that the species breeds during summer and winter. | 2003 | 15162697 |
| the macrophage chemotactic activity of streptococcus agalactiae and streptococcus iniae extracellular products (ecp). | the ability of streptococcus agalactiae and streptococcus iniae to attract macrophages of nile tilapia (oreochromis niloticus) was investigated. the extracellular products (ecp) from s. agalactiae and s. iniae were tested in vitro for macrophage chemotaxis using blind-well chambers. the macrophages were obtained from the peritoneal cavity 4-5 days after intraperitoneal injection of squalene. both macrophage chemotactic and chemokinetic activities were demonstrated using the s. agalactiae ecp. ho ... | 2007 | 17212985 |
| environmental stress and genetic damage in aquatic organisms. | this work was planned to investigate the effect of pollution upon the genetic material of aquatic organisms. in order to achieve such a purpose, tilapia nilotica and tilapia zillii were chosen and caught from two regions varying in their environmental stress; the first is river nile and the second is a closed drain which receives domestic sewage. mitotic activity, chromosomal aberrations and in vivo induction of sister chromatid exchanges were examined in gills as well as in kidneys. the results ... | 1996 | 17214192 |
| microbial mats for multiple applications in aquaculture and bioremediation. | microbial mats occur in nature as stratified communities of cyanobacteria and bacteria, but they can be cultured on large-scale and manipulated for a variety of functions. they are complex systems, but require few external inputs. the functional uses of mats broadly cover the areas of aquaculture and bioremediation. preliminary research also points to promising uses in agriculture and energy production. regarding aquaculture, mats were shown to produce protein, via nitrogen fixation, and were ca ... | 2004 | 15182828 |
| enzymatic responses to metal exposures in a freshwater fish oreochromis niloticus. | freshwater fish oreochromis niloticus were exposed to 5, 10 and 20 microm concentrations of cd, cu, zn and pb for 14 days and responses of several enzymes were determined subsequently. liver catalase (cat) activity was influenced by cd and pb exposures, while it was inhibited by zn exposure. copper, on the other hand, did not cause significant changes in cat activity. liver alkaline phosphatase (ap) activity was first stimulated at lowest (5 microm) exposure concentration, while there were signi ... | 2007 | 17289437 |
| dynamics of cytochrome p450 inducers in polluted sites of são paulo city reservoirs. | the first analysis of water pollutants using biomarkers at the guarapiranga reservoir, which supplies water for one-third of the population of the são paulo megalopolis (brazil), is reported. studies were performed before and after the start of water pumping to the guarapiranga from the highly polluted billings reservoir. billings's water was purified by passing through the natural wetland located near guarapiranga. liver enzymes of oreochromis niloticus (tilopias) obtained from both reservoirs ... | 2004 | 15261731 |
| circulating thyroid hormone levels and iodothyronine deiodinase activities in nile tilapia (oreochromis niloticus) following dietary exposure to endosulfan and aroclor 1254. | we evaluated the effects of two organochlorinated environmental contaminants, endosulfan and aroclor 1254 on peripheral thyroid hormone metabolism and thyroid hormone plasma levels in nile tilapia (oreochromis niloticus). tilapia were exposed through diet to 0.1 and 0.5 microg g(-1) of endosulfan and 0.5 microg g(-1) of aroclor 1254 for 21 and 35 days. decreased plasma t4 and rt3 levels were observed in tilapia exposed to the lower dose of endosulfan, while treatment with a higher dose and arocl ... | 2005 | 15923148 |
| evaluation of river water genotoxicity using the piscine micronucleus test. | the berdan river, which empties into the mediterranean sea on the east coast of turkey, receives discharges of industrial and municipal waste. in the present study, the in vivo piscine micronucleus (mn) test was used to evaluate the genotoxicity of water samples collected from different locations along the berdan river. nile tilapia (oreochromis niloticus) were exposed in the laboratory for 2, 4, and 6 days, and micronuclei were evaluated in peripheral blood erythrocytes, gill cells, and caudal ... | 2007 | 17370337 |
| efficacy of streptococcus agalactiae (group b) vaccine in tilapia (oreochromis niloticus) by intraperitoneal and bath immersion administration. | we evaluated the effectiveness of a streptococcus agalactiae vaccine in tilapia (oreochromis niloticus) for prevention of streptococcal disease. the vaccine was prepared from formalin-killed cells and concentrated extracellular products (greater than 3 kda) of a single isolate of s. agalactiae (ars-ku-mu-11b). intraperitoneal (ip) and bath immersion (bi) vaccine trials were conducted at two temperatures, 32 and 26 degrees c, and mean fish weights, 5 and 30 g. control tilapia were injected with t ... | 2004 | 15315858 |
| evaluation of the link between gyrodactylosis and streptococcosis of nile tilapia, oreochromis niloticus (l.). | streptococcus iniae and gyrodactylus niloticus are two common pathogens of cultured nile tilapia, oreochromis niloticus. we studied concurrent infection of tilapia by g. niloticus and s. iniae and evaluated whether parasitism in tilapia with gyrodactylus increased susceptibility and mortality following immersion infection with s. iniae. results showed that death mainly occurred in fish with g. niloticus and challenged with s. iniae (g-s group). the accumulative mortality (42.2%) was significantl ... | 2007 | 17394525 |
| systemic and mucosal antibody response in tilapia, oreochromis niloticus (l.), following immunization with flavobacterium columnare. | specific antibody responses to flavobacterium columnare (isolate atcc 23463t) were characterized in plasma and mucus of tilapia following intraperitoneal (i.p.) injection or immersion immunization with formalin-killed sonicated or whole cell preparations. fish (30 per treatment) received a primary immunization and were booster immunized 4 weeks later. an enzyme-linked immunosorbent assay was developed for detection and quantification of specific anti-f. columnare antibody, and it was found that ... | 2004 | 15482422 |
| spermatogenesis in testis primary cell cultures of the tilapia (oreochromis niloticus). | spermatogenesis in vertebrates is controlled by endocrine and paracrine factors and involves the communication between somatic and germ line cells. to elucidate some of the relevant factors in the complicated molecular control processes, we established an in vitro test system using primary cultures of tilapia (oreochromis niloticus) testis cells. the cultures were enriched for germ line cells and sertoli cells and largely depleted of spermatozoa. by staining the cells with propidium iodide and c ... | 2005 | 15965976 |
| cloning, expression and characterization of three types of 17beta-hydroxysteroid dehydrogenases from the nile tilapia, oreochromis niloticus. | in order to elucidate the roles of 17beta-hsds in fish gonadal steroidogenesis, three types of 17beta-hsds (17beta-hsd1, 17beta-hsd8 and putative 17beta-hsd12) were cloned and characterized from the nile tilapia, oreochromis niloticus. the cloned cdnas of 17beta-hsd type 1, 8 and 12 were 1504, 1006 and 1930 bp long, with open reading frames encoding proteins of 289, 256 and 314 aminoacids, respectively. tissue distribution pattern analyzed by rt-pcr and northern blot showed that 17beta-hsd1 was ... | 2005 | 16087725 |
| molecular cloning of cellular apoptosis susceptibility (cas) gene in oreochromis niloticus and its proposed role in regulation of non-specific cytotoxic cell (ncc) functions. | cellular apoptosis susceptibility (cas) gene is a homologue of the chromosome segregation gene (cse) in yeast, involved in multiple cellular mechanisms associated with cell proliferation as well as cell death. cas is highly expressed in proliferating cells but at a lower level in quiescent cells and tissues. therefore it appears that cas may play an important role in cancer development. we have previously identified cas in tilapia non-specific cytotoxic cells (ncc) with a cross-reacting monoclon ... | 2006 | 16153859 |
| pesticide residues in nile tilapia (oreochromis niloticus) and nile perch (lates niloticus) from southern lake victoria, tanzania. | nile tilapia (oreochromis niloticus) and nile perch (lates niloticus) samples were collected from fish landing stations in nine riparian districts on the tanzanian side of lake victoria and screened for residues of 64 organochlorine, organophosphorus, carbamate, and pyrethroid pesticides. the residue levels in the fish fillet were up to 0.003, 0.03 and 0.2 mg/kg fresh weight (0.7, 3.8 and 42 mg/kg lipid weight) of fenitrothion, ddt and endosulfan, respectively. mean levels within sites were up t ... | 2006 | 16199118 |
| differential expression of tuberoinfundibular peptide 38 and glucose-6-phosphatase in tilapia. | a new parathyroid hormone (pth)-like endocrine system has been identified in mammals and fishes consisting of the pth type-2 receptor (pth2r) and tuberoinfundibular peptide 39 (tip39). although the mammalian pth2r-tip39 system is involved in nociception and pituitary regulation, the function(s) of this system in fishes is undetermined. using degenerate primers based on conserved zebrafish and fugu tip39 nucleotide sequences, 3'-race reactions isolated the coding region of a putative tip38 cdna i ... | 2006 | 16375902 |
| depuration of microcystins in tilapia fish exposed to natural populations of toxic cyanobacteria: a laboratory study. | previous studies demonstrated that microcystins (mcysts) can be accumulated in different organs, particularly the liver, of tilapia fish (oreochromis niloticus) in an egyptian fish farm containing toxic blooms of microcystis aeruginosa. in the present study, a microcosm experiment was conducted to examine the depuration of mcysts in tilapia fish from this fish farm. fish were grown in a 100-l aerated recirculation tank containing dechlorinated water at room temperature (25+/-2 degrees c) for 96 ... | 2006 | 16406591 |
| things we have learned from tilapia islet xenotransplantation. | an islet xenotransplantation model has been developed using tilapia (oreochromis niloticus) as the donors. studies using this model for the treatment of experimental type 1 diabetes in mice have produced promising results including the maintenance of long-term normoglycemia and mammalian-like glucose tolerance profiles in islet graft recipients. islet encapsulation has also provided a promising method for the prevention of graft rejection, and strains of transgenic tilapia expressing a [desthrb3 ... | 2006 | 16413551 |
| malaria mosquito control using edible fish in western kenya: preliminary findings of a controlled study. | biological control methods are once again being given much research focus for malaria vector control. this is largely due to the emerging threat of strong resistance to pesticides. larvivorous fish have been used for over 100 years in mosquito control and many species have proved effective. in the western kenyan highlands the larvivorous fish oreochromis niloticus l. (perciformes: cichlidae) (formerly tilapia nilotica) is commonly farmed and eaten but has not been previously tested in the field ... | 2007 | 17688686 |
| organochlorine residues in fish and water samples from lake victoria, uganda. | nile tilapia and nile perch samples from lake victoria were analyzed for lindane (gamma-1,2,3,4,5,6-hexachlorocyclohexane), aldrin (1,2,3,4,10,10-hexachloro-1,4,4a,5,8,8a-hexahydro-1,4:5,8-dimethanonaphthalene), alpha-endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3- benzo(e) dioxathiepin-3-oxide), dieldrin (1,2,3,4,10,10-hexachloro-6,7-epoxy-1,4,4a,5,6,7,8,8a-octahydro-1,4,5,8-dimethanonaphthalene), dde (p,p'-1,1-dichloro-2,2-bis-(4-chlorophenyl)ethylene), and ddt ... | 2006 | 16510703 |
| promoter activity and chromosomal location of the rana rugosa p450 aromatase (cyp19) gene. | sex is determined genetically in amphibians, but is reversed occasionally by steroid hormones. the phenotypic sex of some amphibian species can be reversed from male to female by estrogens. estrogens, which are synthesized from testosterone irreversibly by the enzyme p450 aromatase (cyp19), are essential for ovarian development in vertebrates. cyp19 expression is reportedly regulated by steroidogenic factor-1 (sf-1), also designated as ad4bp, in fish and mammals. however, it is unknown if this i ... | 2006 | 16547409 |
| response of catalase activity to ag+, cd2+, cr6+, cu2+ and zn2+ in five tissues of freshwater fish oreochromis niloticus. | catalase (cat, ec 1.11.1.6) is an important enzyme in antioxidant defense system protecting animals from oxidative stress. freshwater fish oreochromis niloticus were exposed for 96 h to different concentrations of ag(+), cd(2+), cr(6+), cu(2+) and zn(2+), known to cause oxidative stress, and subsequently cat activities in liver, kidney, gill, intestine and brain were measured. in vivo, cat was stimulated by all metals except ag(+) in the liver and the highest increase in cat activity (183%) resu ... | 2006 | 16581305 |
| freshwater microcosms-based assessment of eco-toxicological effects of a chemical effluent from the pilcam industry in cameroon. | we studied the acute toxicity of a raw effluent from a battery manufacturing plant (pilcam) in douala, cameroon, to a freshwater fish (oreochromis niloticus), and subsequently evaluated its sub-acute effects on water quality and the biota in freshwater microscosms. the acute toxicity test was based on 96 hrs static renewal bioassays that resulted in 96-h lc50 and lc90 values of 16 and 20.7% (v/v), respectively. the sub-acute experiments were conducted by exposing several species of aquatic organ ... | 2004 | 16696186 |
| food safety aspects of toxic element accumulation in fish from wastewater-fed ponds in hanoi, vietnam. | to evaluate the food safety of fish from production systems in hanoi feed with domestic and industrial wastewater with respect to the potentially toxic elements such as arsenic (as), cadmium (cd) and lead (pb). | 2007 | 18005313 |
| occurrence and species distribution of fishborne zoonotic trematodes in wastewater-fed aquaculture in northern vietnam. | to assess the risk of fishborne zoonotic trematode (fzt) infection in fish reared in wastewater-fed ponds in peri-urban areas of northern vietnam. | 2007 | 18005317 |
| body size-related differences in the inhibition of brain acetylcholinesterase activity in juvenile nile tilapia (oreochromis niloticus) by chlorpyrifos and carbosulfan. | influence of body size on inhibition of brain acetylcholinesterase (ache) activity of juvenile nile tilapia, oreochromis niloticus by chlorpyrifos and carbosulfan was investigated concerning its potential use in the biomonitoring of anticholinesterase pesticides in tropical water bodies. three size groups of fish (fry: 3-4 cm, fingerlings: 6-8 cm, sub-adults: 10-12 cm in total length) were exposed to a series of concentrations of chlorpyrifos (0.5-12 microg l(-1)) or carbosulfan (1-10 microg l(- ... | 2007 | 16753212 |
| polyunsaturated fatty acid content of wild and farmed tilapias in thailand: effect of aquaculture practices and implications for human nutrition. | the total lipid content and fatty acid composition of the muscle tissue of tilapia (oreochromis niloticus) and of hybrid red tilapia (oreochromis sp.) from different culture systems and from the natural and artificial environment of thailand were compared. wild fish and fish reared under the most extensive conditions had a more favorable fatty acid profile for human consumption as they contained higher proportions of 18:3n-3, 20:5n-3, and 22:6n-3, higher n-3/n-6 pufa ratios, and lower proportion ... | 2006 | 16756360 |
| pollution assessment of the lower basin of lakes kainji/jebba, nigeria: heavy metal status of the waters, sediments and fishes. | the objective of this investigation was to examine the heavy metal status of the lower basin of kainji dam (used for hydroelectricity generation), which includes lakes kainji/jebba, nigeria, and the potential for human exposure to heavy metals from eating fish caught in the lakes. water, sediments and fish were sampled from the lakes and evaluated for as, cu, co, cr, fe, hg, mn, ni, pb, sb, ti, v and zn using the edxrf technique. fe and mn were found to be present at high mean concentrations in ... | 2006 | 16767565 |
| a piscirickettsiosis-like syndrome in cultured nile tilapia in latin america with francisella spp. as the pathogenic agent. | in 2004, cultured nile tilapia oreochromis niloticus in several latin america farms began to succumb to a disease similar to the piscirickettsiosis-like syndrome previously reported in tilapia in taiwan and the united states. mortality increased during 2005; reductions in tilapia biomass ranged from 5% to 80% in individual ponds and averaged 50% overall. all ages of fish have been involved. clinical signs include lethargy, loss of appetite, petechia, exophthalmia, and abnormal swimming behavior. ... | 2007 | 18236629 |
| structural and functional characterization of microcystin detoxification-related liver genes in a phytoplanktivorous fish, nile tilapia (oreochromis niloticus). | liver genes related to phase i and phase ii detoxification, as well as inhibition of reactive oxygen species (ros) production, were cloned, and their response to microcystin-lr (mc-lr) and lipopolysaccharide (lps) exposure via intraperitoneal injection, was determined in a phytoplanktivorous fish, nile tilapia (oreochromis niloticus). the cloned full-length cdna of tilapia soluble glutathione s-transferase (sgst) was classified as alpha-class gst based on their amino acid sequence identity with ... | 2006 | 17045849 |
| effect of repeated application of fenthion as a mosquito larvicide on nile tilapia (oreochromis niloticus) inhabiting selected water canals in sri lanka. | health status of feral nile tilapia following repeated applications of fenthion as a mosquito larvicide to selected water canals in sri lanka was assessed. with three spray applications of fenthion to the study sites at weekly intervals at the concentration recommended for mosquito control, condition factor and brain acetylcholinesterase activity of the fish were depressed in a time dependent manner. prominent histopathological alterations displayed were gill hyperplasia and telangiectasis and v ... | 2008 | 18344014 |
| the macrophage chemotactic activity of edwardsiella tarda extracellular products. | the chemoattractant capabilities of edwardsiella tarda extracellular products (ecp) were investigated from two isolates, the virulent fl6-60 parent and less virulent ret-04 mutant. chemotaxis and chemokinesis were assayed in vitro using blind well chambers with peritoneal macrophages obtained from nile tilapia, oreochromis niloticus, 5 days following squalene injection. non-purified ecp derived from both isolates stimulated predominantly chemokinetic migration of macrophages. additionally, the e ... | 2008 | 18355182 |
| natural infection of fresh and brackish water fish with heterophyid encysted metacercariae in dakahlia governorate, egypt. | the encysted metacercariae (emc) of heterophyids in the fish species; mugil caphalus, m. capito, m. auratus, oreochromis n. niloticus (=tilapia nilotica), and tilapia zillii trapped brackish and fresh water fishes were isolated by the artificial digestion method and identified. the emc by morphometric analysis proved to be seven phenotypical distinct types. cross-matched seven groups of clean laboratory bred puppies each was experimentally infected with a distinct emc type. the recovered adult f ... | 2007 | 18383799 |
| stress responses of the fish nile tilapia subjected to electroshock and social stressors. | plasma cortisol and glucose levels were measured in 36 adult nile tilapia males, oreochromis niloticus (standard length, mean +/- sd, 14.38 +/- 1.31 cm), subjected to electroshock and social stressors. pre-stressor levels were determined 5 days after the adjustment of the fish to the experimental aquaria (1 fish/aquarium). five days later, the effects of stressors on both cortisol and glucose levels were assessed. the following stressors were imposed for 60 min: pairing with a larger resident an ... | 2006 | 17160270 |
| protective role of vitamin e on the microcystin-induced oxidative stress in tilapia fish (oreochromis niloticus). | microcystins (mcs) are potent hepatotoxins produced by cyanobacteria in water systems that induce oxidative stress in fish. the present study investigated the effect of vitamin e pretreatment on mc-induced oxidative damage in the liver, kidneys, and gills of tilapia fish (oreochromis niloticus). groups of fish were fed vitamin e supplements (200 or 700 mg per kilogram of diet) for 7 d or received only commercial fish food and then were exposed to a single oral dose of cyanobacterial cells (120 m ... | 2008 | 18419199 |
| protective immunity of nile tilapia against ichthyophthirius multifiliis post-immunization with live theronts and sonicated trophonts. | two immunization trials were conducted to evaluate host protection of nile tilapia, oreochromis niloticus against ichthyophthirius multifiliis (ich). immunizations were done with live theronts or sonicated trophonts by bath immersion and intraperitoneal (ip) injection. the immunized fish were challenged with theronts 21 days post-immunization in trial i and 180 days post-immunization in trial ii. the serum anti-ich antibody and cumulative mortalities of tilapia were determined after theront chal ... | 2008 | 18442922 |
| lipids and fatty acids composition in bolti (tilapia nilotica) fish flesh as affected by environment. | bolti (tilapia nilotica) fish and water samples were taken from three different locations in kafr el-sheikh governorate. water quality and oil characteristics in fish flesh were studied. the obtained results showed that crude lipid and phospholipid contents of fish fillet were increased by increasing total solids and total hardness of fish environment. peroxide, iodine and acid values of fish oil influenced by water quality. fatty acids composition in fish oil showed a wide variation due to diff ... | 1995 | 17214202 |
| acute toxicity of euphorbia kamerunica on oreochromis niloticus. | a series of static short-term bioassays was conducted to assess the acute toxicity of the plant euphorbia kamerunica on the fingerlings of oreochromis niloticus. the 96-h lc(50) was computed using two methods: probit analysis and the arithmetic graphic method. the overall mean value of 0.023 +/- 0.002 g/l was obtained. aquatic invertebrates are generally much more sensitive than fish species. poisoning of streams with this plant to capture fish may therefore have ecological consequences, as nont ... | 2004 | 15978298 |
| molecular and functional characterization of a cd59 analogue from large yellow croaker pseudosciana crocea. | cd59 is a widely distributed membrane-bound inhibitor of the cytolytic membrane attack complex (mac) of complement. here, the cdna of a cd59 analogue was cloned from large yellow croaker (pseudosciana crocea), a marine fish (lyccd59), by expressed sequence tags (est) and race techniques. the open reading frame (orf) of 351 nucleotides (nt) of lyccd59 encodes a polypeptide of 117 amino acids (aa), which includes a putative 20-aa nh(2)-signal peptide and a 97-aa coding region with a putative gpi-a ... | 2007 | 17531319 |
| induced cytochrome p450 1a activity in cichlid fishes from guandu river and jacarepaguá lake, rio de janeiro, brazil. | the induction of cytochrome p4501a-mediated activity (e.g. ethoxyresorufin-o-deethylation, erod) has been used as a biomarker for monitoring fish exposure to ahr-receptor ligands such as polycyclic aromatic hydrocarbons (pah), polychlorinated biphenyls (pcb) and polychlorinated dibenzo-dioxins/furans (pcdd/fs). in this study we found that hepatic erod is induced in fish ("nile tilapia", oreochromis niloticus and "acará", geophagus brasiliensis) from the guandu river (7-17-fold) and jacarepaguá l ... | 2008 | 17602810 |
| biosafety of recombinant and wild type nucleopolyhedroviruses as bioinsecticides. | the entomopathogenic autographa californica (speyer) nucleopolyhedrovirus (acmnpv) has been genetically modified to increase its speed of kill. the potential adverse effects of a recombinant acmnpv (acaait) as well as wild type acmnpv and wild type spodoptera littoralis npv (slnpv) were studied. cotton plants were treated with these viruses at concentrations that were adjusted to resemble the recommended field application rate (4 x 10(12) pibs/feddan, feddan = 4,200 m2) and 3rd instar larvae of ... | 2007 | 17617674 |
| distinct repressive properties of the mammalian and fish orphan nuclear receptors shp and dax-1. | it has been suggested that the structure and function of nuclear receptors are evolutionally conserved. here, we compare the molecular functions of the nile tilapia (oreochromis niloticus) small heterodimer partner (nshp/nr0b2) and the dosage-sensitive sex reversal ahc critical region on x chromosome gene 1 (ndax-1/nr0b1) with those of human shp and dax-1 (hshp and hdax-1, respectively). we found that, upon transient cotransfection of human cells, ndax-1 repressed the activity of tilapia sf-1 (n ... | 2007 | 17646707 |
| time-dependent oxidative stress responses after acute exposure to toxic cyanobacterial cells containing microcystins in tilapia fish (oreochromis niloticus) under laboratory conditions. | microcystins (mcs) have been reported to induce oxidative stress in aquatic organisms including fish. the effect of acute exposure to toxic cyanobacterial material containing mcs on antioxidant enzymes and lipid peroxidation has been studied in liver, kidney and gills of tilapia fish (oreochromis niloticus). fish were orally exposed to a single dose of cyanobacterial cells containing 120 microg/fish mc-lr and sacrificed at 24 and 72 h. the activities of glutathione peroxidase (gpx), glutathione ... | 2007 | 17669513 |
| backbone cyclised peptides from plants show molluscicidal activity against the rice pest pomacea canaliculata (golden apple snail). | golden apple snails ( pomacea canaliculata) are serious pests of rice in south east asia. cyclotides are backbone cyclized peptides produced by plants from rubiaceae and violaceae. in this study, we investigated the molluscicidal activity of cyclotides against golden apple snails. crude cyclotide extracts from both oldenlandia affinis and viola odorata plants showed molluscicidal activity comparable to the synthetic molluscicide metaldehyde. individual cyclotides from each extract demonstrated a ... | 2008 | 18557620 |
| prevalence of zoonotic trematodes in fish from a vietnamese fish-farming community. | the prevalence of fish-borne zoonotic trematode (fzt) metacercariae was investigated in fish farmed by rural households in nghe an province, located in northern vietnam. in total, 716 fish, including tilapia (oreochromis niloticus) and 6 carp species, i.e., grass carp (ctenopharyngodon idellus), bighead carp (aristichthys nobilis), mrigal (cirrhinus mrigala), common carp (cyprinus carpio), silver carp (hypophthalmychthys molitrix), and roha (labeo rohita), collected from 53 fish farms were exami ... | 2008 | 18564743 |
| new exogenous stages of oocysts, sporocysts, and sporozoites of goussia cichlidarum landsberg and paperna 1985 (sporozoa: coccidia) and impact of endogenous stages on the swim bladder of tilapias in egypt. | obviously, the present study reports the coccidian parasite so-called goussia cichlidarum for the first time in egypt. altogether, 25 exogenous stages were clearly distinguished from specimens of naturally infected fishes of oreochromis niloticus, o. auraeus, and tilapia zillii from different locations. the total prevalence of infection was about 41%. mostly, infected fish grossly seemed with a healthy body, although severe lesions have been detected microscopically in massive infection. portion ... | 2008 | 17885764 |
| a revised description of gyrodactylus cichlidarum paperna, 1968 (gyrodactylidae) from the nile tilapia, oreochromis niloticus niloticus (cichlidae), and its synonymy with g. niloticus cone, arthur et bondad-reantaso, 1995. | a recent infestation of gyrodactylus cichlidarum paperna, 1968 on yolk sac fry of nile tilapia, oreochromis niloticus niloticus (l.), in an isolated aquarium system in the u.k. resulted in high mortalities and provided an opportunity to study this species in greater detail. a tentative identification was made using the measurements and drawings of the ventral bar and hamuli provided in the original description; however, details on the morphology of the marginal hooks were lacking. a comparison o ... | 2007 | 17886742 |
| acute toxicity and cytotoxicity of bacillus thuringiensis and bacillus sphaericus strains on fish and mouse bone marrow. | the insecticidal properties of delta-endotoxins from bacillus thuringiensis (bt) serotypes kurstaki and israelensis and crystal proteins of bacillus sphaericus (bs) serotype h5 have been used in insect control for decades. the availability of microbial toxins in biopesticides as well as in plants with incorporated protection has been increasing the concerns about biosafety. acute toxicity to danio rerio and cytotoxicity on mouse bone marrow cells and peripheral erythrocytes of oreochromis niloti ... | 2009 | 18670879 |
| oxidative stress biomarkers of exposure in the blood of cichlid species from a metal-contaminated river. | the oxidative stress biomarkers of exposure, such as reduced glutathione (gsh), activity of superoxide dismutase (sod), catalase (cat) and glutathione peroxidase (gpx) and the levels of lipid peroxidation (lpo), were measured in the blood of three cichlid fish (oreochromis niloticus, tilapia rendalli, and geophagus brasiliensis) taken during two seasons from two sites, unpolluted and polluted by industrial effluents, to evaluate the effectiveness of these biomarkers in assessing the impact of wa ... | 2008 | 17936357 |
| use of biomarkers in nile tilapia (oreochromis niloticus) to assess the impacts of pollution in bolgoda lake, an urban water body in sri lanka. | the present study reports the first analysis of water pollutants in sri lankan waters using a suite of biomarkers in nile tilapia (oreochromis niloticus) residing in bolgoda lake which receives urban, industrial and domestic wastes from multiple sources. the fish were collected from the lake in the dry period (april 2005) and wet periods (september 2005, october 2006) and the levels of biomarkers viz. hepatic ethoxyresorufin o-deethylase (erod), glutathione s-transferase (gst), metallothioneins, ... | 2009 | 18688734 |
| tilapia larvae aroclor 1254 exposure: effects on gonads and circulating thyroid hormones during adulthood. | aroclor 1254 a polychlorinated biphenyls (pcbs) mixture, when administrated through the diet, was previously found to inhibit adult tilapia (oreochromis niloticus) reproduction. since fish larvae can be more sensitive to contaminants, the objectives of this study were to evaluate in adults the impact in gonad development and in thyroid function of aroclor 1254 administrated at larvae stages. aroclor 1254 exposed tilapia presented both ovary and testicular alterations and a decline in t4 plasma c ... | 2007 | 17943219 |
| microcystin accumulation in liver and muscle of tilapia in two large brazilian hydroelectric reservoirs. | the objective of this study was to measure levels of the toxin microcystin in different tissues of fish known to feed on cyanobacteria during toxic bloom events. wild nile and redbreast tilapia (oreochromis niloticus and tilapia rendalli) were sampled from the catch of artisanal fishermen at eutrophic stations of funil and furnas reservoirs in southeastern brazil. phytoplankton communities in the two reservoirs were quite different taxonomically, but not dissimilar in microcystin content (200 mi ... | 2008 | 18067935 |
| vitellogenin induction and increased plasma 17beta-estradiol concentrations in male nile tilapia, oreochromis niloticus, exposed to organochlorine pollutants and polycyclic aromatics hydrocarbons. | vitellogenin (vtg), 17beta-estradiol (e2) and testosterone (t) were used as biomarkers of endocrine disruption in mature male nile tilapia (oreochromis niloticus) from three lakes (rio, enmedio and limon) in chiapas, mexico. vitellogenesis induction was found in tilapias from rio and limon, moderately high e(2) levels in rio and limon tilapias, compared with controls (cultured tilapias). significant correlations between benzo(a)pyrene (bap) metabolites and hexachlorobenzene (hcb) with vtg and e( ... | 2008 | 18787751 |
| molecular cloning of plk1 and nek2 and their expression in mature gonads of the teleost fish nile tilapia (oreochromis niloticus). | polo-like kinase 1 (plk1) and nima-related kinase 2 (nek2) are serine/threonine kinases that are involved in the g2/m phase transition of the cell cycle in vertebrates. although they also play critical roles in the regulation of spermatogenesis and oogenesis, their characterization in meiosis has not been elucidated fully, particularly in teleost fish. to investigate the evolutionary significance of serine/threonine kinases in the reproductive system of fish, we cloned the cdnas of plk1 and nek2 ... | 2008 | 18095313 |
| purification and localization of nitric oxide synthases from hybrid tilapia (nile tilapia x mozambique tilapia). | the aims of this study were to purify and localize the nitric oxide synthases (noss) from hybrid tilapia (nile tilapia oreochromis niloticus x mozambique tilapia o. mossambicus). the purification procedures involved affinity chromatography with a 2', 5'-adp-agarose 4b column and ion exchange with a diethylaminoethanol bio-gel a column. the results from gel filtration assays showed that the molecular weights of neuronal nos (nnos) and inducible nos (inos) were 178 and 120 kda, respectively. the s ... | 2007 | 18201058 |
| evaluation of remediation of coal mining wastewater by chitosan microspheres using biomarkers. | acidic mine waters have a marked influence on the surrounding environment and pose a serious threat through long-term environmental degradation. therefore, it is important to improve and monitor water quality with the aim of decreasing the hazard presented by this effluent emission. the aim of this work was to evaluate the remediation of mining wastewater effluents by chitosan microspheres using biomarkers of exposure and effect. dna damage (comet assay) and several biomarkers of oxidative stres ... | 2006 | 16988869 |
| income evaluation of small - scale fishers in two brazilian urban reservoirs: represa billings (sp) and lago paranoá (df). | the income of small-scale fisheries of two urban reservoirs in brazil: represa billings (127 km2) located in the metropolitan region of são paulo, the capital city of the state of são paulo and lago paranoá (38 km2) located in brasília (df), the capital city of brazil were calculated and compared. both fisheries are mainly based on the alien nile tilapia oreochromis niloticus (more than 80% of the total catch). although these reservoirs are nearly 900 km apart, their native fish fauna belong to ... | 2006 | 17119829 |
| [effect of the probiotic bacillus subtilis on the growth and food utilization of tilapia (oreochromis niloticus) and prawn (macrobrachium rosenbergii) under laboratory conditions]. | three experiments were conducted to analyze the effect of the probiotic bacillus subtilis on the growth of juvenile tilapia (oreochromis niloticus) and freshwater prawn (macrobrachium rosenbergii). the experiments were conducted under laboratory conditions, minimizing the indirect effects of the probiotic on the water quality and leaving only the possible bactericidal and digestion-support effects. a model of stress was also designed in tilapia to compare the effect with tilapia under normal con ... | 2004 | 17354405 |
| reproductive biology of oreochromis niloticus (perciformes: cichlidae) at emiliano zapata dam, morelos, mexico. | the reproductive season of tilapia was studied by monthly samplings at emiliano zapata dam, morelos state, mexico. from february 1999 through february 2000 a sample of 50 fish was taken from the commercial catch (castnet, 6.5 cm of mesh size). the observed sex ratio was 1:1.29 (females:males) (chi(2)=10.26; p<0.05). the tilapia reached maturity at 151.3 mm (females) and 152.0 mm (males) of total length. rainy (august) and dry (february) seasons were determined as the breeding period. fecundity v ... | 2005 | 17354460 |
| induction of oxidative stress and apoptosis by pfos and pfoa in primary cultured hepatocytes of freshwater tilapia (oreochromis niloticus). | perfluorinated organic compounds (pfocs) are emerging persistent organic pollutants (pops) widely present in the environment, wildlife and human. we studied the cellular toxicology of perfluorooctane sulfonate (pfos) and perfluorooctanoic acid (pfoa) on oxidative stress and induction of apoptosis in primary cultured hepatocytes of freshwater tilapia (oreochromis niloticus). cultured hepatocytes were exposed to pfos or pfoa (0, 1, 5, 15 and 30 mg l(-1)) for 24h, and a dose-dependent decrease in c ... | 2007 | 17374408 |
| evaluation of the influence of housefly maggot meal (magmeal) diets on catalase, glutathione s-transferase and glycogen concentration in the liver of oreochromis niloticus fingerling. | influence of housefly maggot meal (magmeal) diets on the activities of catalase (cat), glutathione s-transferase (gst) and glycogen concentration in liver of tilapia oreochromis niloticus fingerling was evaluated. triplicate groups of fifteen fish (initial average weight 2.0+/-0.1 g) were fed eight weeks with seven test diets (in average 36% crude protein, dry matter) formulated by replacing fish meal with magmeal. percentage body weight gain (591-724.46%), food conversion ratio (1.05-1.22) and ... | 2007 | 17400494 |
| natural origins of off-flavours in fish related to feeding habits. | the objective of our study was to survey the accumulated 2-methylisoborneol (mib) and geosmin (gsm) in fillets of five important farmed fish species in hungarian aquaculture in relation to mib and gsm concentrations in water and sediment in the aquatic systems where they were raised: (the planktivorous silver carp (hypophthalmichthys molitrix), the herbivorous grass carp (ctenopharyngodon idella), the bottom-feeding omnivorous common carp (cyprinus carpio), the omnivorous tilapia (oreochromis ni ... | 2007 | 17489423 |
| induction of cytochrome p450 1a1 as a biomarker of benzo-a-pyrene pollution in egyptian fresh water fish. | the activity of ethoxyresorufin-o-dealkylase (erod) in the liver of oreochromis niloticus and clarias gariepinus was evaluated as a response to experimental and natural contamination of water with benzo-a-pyrene and/or cadmium. the activity was measured fluorimetrically in the hepatic s9 fraction while the content of the enzyme was measured by elisa. the response appeared as early as six hours post exposure. this study also reveled that oreochromis niloticus exhibits higher values of erod activi ... | 2007 | 19069910 |
| effects of dietary selenium on the oxidative stress and pathological changes in tilapia (oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom. | the present study investigates the role of selenium (se) supplementation (as sodium selenite) on the oxidative stress and histopathological changes induced by cyanobacterial cells containing microcystins (mcs) in tilapia fish (oreochromis niloticus). variation in lipid peroxidation (lpo) levels and carbonyl groups content, reduced glutathione/oxidized glutathione (gsh/gssg) ratio, and catalase (cat), superoxide dismutase (sod), glutathione reductase (gr), glutathione peroxidase (gpx) and glutath ... | 2009 | 19073206 |
| development of specific enzyme-linked immunosorbent assay for determining lh and fsh levels in tilapia, using recombinant gonadotropins. | we recently produced oreochromis niloticus recombinant lh and fsh as single-chain polypeptides in the methylotrophic yeast pichia pastoris. glycoprotein subunit alpha was joined with tilapia (t) lhbeta or tfshbeta mature protein-coding sequences to form a fusion gene that encodes a ;;tethered" polypeptide, in which the gonadotropin beta-subunit forms the n-terminal part and the alpha-subunit forms the c-terminal part. recombinant (r) gonadotropins were used to develop specific and homologous com ... | 2007 | 17507016 |
| recombinant novel pituitary adenylate cyclase-activating polypeptide from african catfish (clarias gariepinus) authenticates its biological function as a growth-promoting factor in low vertebrates. | nowadays, the studies of pituitary adenylate cyclase-activating polypeptide (pacap)-related peptide (prp) and pacap in non-mammalian vertebrates, especially in fish, have paid attention mainly to the localization, cloning, and structural evolution of the peptides, but very little is known about its biological functions as growth-promoting factors in low vertebrates. in this work, we have cloned and characterized the prp/pacap cdna from the commercially important north african catfish clarias gar ... | 2008 | 18492822 |
| angling sport fishing in lobo-broa reservoir (itirapina, sp, brazil). | the objective of this paper was to study angling from september, 2002 to september, 2004 in the lobo-broa reservoir, itirapina, sp (22 degrees 15' s and 47 degrees 49' w). interviews (total 1,027) with sport fishers were accomplished in the three main fishing sites (horto, píer and praia). this fishing was practiced with a simple fishing rod and reel, mainly in horto, where the catches and fishing effort were higher. the catches were mainly composed of cichlidae (geophagus brasiliensis, oreochro ... | 2008 | 19197489 |
| light and electron microscopic study on henneguya suprabranchiae landsberg, 1987 (myxozoa: myxosporea) infecting oreochromis niloticus, a new host record. | out of 58 live tilapia fish, five oreochromis niloticus were found to be naturally infected with henneguya suprabranchiae (8.62%). such infection was recorded only during winter season from bahr shebin, a tributary of the river nile at menoufia governorate, nile delta, egypt. based on the structure and measurements of fresh spores, this parasite was identified as h. suprabranchiae. spores are oval in shape and they measure 15 (13-16) x 5 (4-6) microm length by width. it has two polar capsules in ... | 2008 | 18516619 |
| seabream gnrh immunoreactivity in brain and pituitary of xx and xy nile tilapia, oreochromis niloticus during early development. | seabream gonadotropin-releasing hormone (sbgnrh)-the chief preoptic area-hypothalamus (poa-h) form of gnrh in tilapia is involved in sexual maturation. in this study, we investigated the qualitative changes in ontogeny of sbgnrh immunoreactivity (ir-), between sexes to understand its impending role during sex differentiation. for this, the differences in immunocytochemical localization of sbgnrh in genetically male (xy) and female (xx) fish were studied from 1 day after hatching (dah), through t ... | 2008 | 18521911 |
| preliminary observations on infection of brackish and fresh water fish by heterophyid encysted metacercariae in egypt. | the infection status with heterophyids in the second intermediate host fish obtained from brackish and fresh water resources in dakahlia governorate, northern egypt was investigated. encysted metacercariae (emc) in the fish species mugil cephalus, mugil capito, mugil auratus, tilapia nilotica, and tilapia zilli from brackish water and t. nilotica and t. zilli from fresh water were isolated by artificial digestion method and identified by microscopic examination. morphometric analysis of emc isol ... | 2008 | 18537032 |
| protein enrichment of apple pomace and use in feed for nile tilapia. | the purpose of this paper is to investigate the protein enrichment of apple pomace by gongronella butleri through solid-state cultivation and addition of this material as feed for tilapia fry (oreochromis niloticus). factorial experimental design was used for the assessment of culture conditions to determine the effects of the source of nitrogen, initial moisture, and granulometry on the protein enrichment of apple pomace. during culture, the consumption of reducing sugars and the production of ... | 2009 | 18600303 |
| effect of treatment with probiotics as water additives on tilapia (oreochromis niloticus) growth performance and immune response. | a feeding trial was conducted for 40 days to delineate the effect of treatment with probiotics as water additives on tilapia (oreochromis niloticus) growth performance and immune response. about 360 juveniles were randomly distributed into four treatment groups, each with three replicates. different probiotics (t-1, bacillus subtilis b10; t-2, bacillus coagulans b16; t-3, rhodopseudomonas palustris g06) were added to the water of tanks at final concentration of 1 x 10(7) cfu ml(-1) every 2 days, ... | 2010 | 19363655 |
| orexin-b-like immunoreactivity localized in both luteinizing hormone- and thyroid-stimulating hormone-containing cells in the nile tilapia (oreochromis niloticus) pituitary. | immunohistochemical techniques were employed to examine orexin-like immunoreactivities in the pituitary of the nile tilapia (oreochromis niloticus). rabbit anti-orexin-a serum and mouse anti-orexin-b monoclonal antibodies were used as primary antibodies. orexin-b immunoreactive cells corresponded to luteinizing hormone (lh)- or thyroid-stimulating hormone (tsh)-containing cells, and all lh- and tsh-containing cells were immunoreactive for orexin-b. however, we found no orexin-a immunoreactive ce ... | 2009 | 18656218 |
| five new myxosporean species (myxozoa: myxosporea) infecting the nile tilapia oreochromis niloticus in bahr shebin, nile tributary, nile delta, egypt. | five new myxosporean species belonging to three different genera were described from the nile tilapia oreochromis niloticus in bahr shebin, nile tributary, nile delta, egypt. these species are: zschokkella nilei sp. n., ortholinea africanus sp. n., triangula egyptica sp. n., myxobolus fomenai sp. n., and myxobolus branchiophilus sp. n. morphometry, light microscopy, and hand drawing of mature spores and plasmodia were presented for each species. | 2008 | 18688646 |
| variation in fatty acid composition in muscle and heart tissues among species and populations of tropical fish in lakes victoria and kyoga. | the composition of the fatty acids in muscle and heart tissue of seven fish species, nile perch (lates niloticus), nile tilapia (oreochromis niloticus), marbled lungfish (protopterus aethiopicus), african catfish (clarias gariepinus), lake victoria squeaker (synodontis victoriae), bagrus docmas, and tilapia zilli, from two locations in lake kyoga and one location in lake victoria was chemometrically determined. the muscle tissue was very lean, with an average of 3.4 mg total fatty acids per g ti ... | 2008 | 18712426 |
| human streptococcus agalactiae isolate in nile tilapia (oreochromis niloticus). | streptococcus agalactiae, the lancefield group b streptococcus (gbs) long recognized as a mammalian pathogen, is an emerging concern with regard to fish. we show that a gbs serotype ia multilocus sequence type st-7 isolate from a clinical case of human neonatal meningitis caused disease and death in nile tilapia (oreochromis niloticus). | 2009 | 19402966 |
| diplostomiasis in cultured and wild tilapia oreochromis niloticus in guerrero state, mexico. | this paper is a comparative study of diplostomum (austrodiplostomum) compactum (lutz, 1928) in nile tilapia oreochromis niloticus (linneo) from two fish farms and two nearby coastal lagoons in guerrero state, mexico. the higher infections levels in cultured tilapia than wild tilapia is attributed to higher fish densities in the culture systems and higher abundance of the snail biomphalaria cf. havanensis (pteiffer), first intermediate host of this parasite in freshwater and brackish water system ... | 2009 | 19452167 |
| leukocyte response and phagocytic activity in nile tilapia experimentally infected with enterococcus sp. | this study evaluated the total and differential leukocyte counting and the phagocytic activity in nile tilapia oreochromis niloticus experimentally injected with enterococcus sp. in the swim bladder. fish were distributed in four treatments in triplicates of non-injected fish, fish injected with 1 ml of sterile saline solution 0.65%, and fish injected with 1 x 10(3) and 1 x 10(6) colony-forming units (cfu) of enterococcus diluted in 1 ml sterile saline. twenty-four hours after injection, the fis ... | 2009 | 18777139 |
| oxyeleotris marmoratus, predator or by-product in integrated aquaculture ponds. | participating farms in the muang district of nong khai province, were selected for the investigation of management practices and yields of marble goby, oxyeleotris marmoratus, in integrated culture with nile tilapia (oreochromis niloticus). the research took place from february to may, 2007. the observed production of o. marmoratus in integrated aquaculture ponds indicated a wide range of management practices. the yields of oxyeleotris marmoratus were between 22.1 to 316 kg ha(-1) (average 81+/- ... | 2008 | 18817122 |