| biochemical toxicology of lindane and its analogs. | the inhibitory potencies of bhc isomers against na+-k+- atpase, yeast growth and nerve conduction of the american cockroach were determined. the insecticidal and neuroexcitatory activities of a number of analogs with the same configuration as lindane in which some chlorine atoms had been replaced by other substituents were also examined against american cockroaches. the structure-activity relationships and the mode of action of lindane and its analogs were discussed. their metabolic fate was stu ... | 1983 | 6187795 |
| the capacity of insect flight muscle for the intracellular transport of phospholipids. | the postmitochondrial supernatant of the homogenate of metathoracic musculature of periplaneta americana was found to mediate the transfer of labelled phosphatidylcholine and phosphatidylinositol from the rat liver microsomal fraction to unlabelled rat liver mitochondria at 25 degrees c; no transfer occurred at 0 degrees c. the transfer of phosphatidylethanolamine by the insect muscle extract could not be demonstrated conclusively. the transfer activity per mg protein of the supernatant fraction ... | 1982 | 6214807 |
| phylogenetic distribution of [3h]kainic acid receptor binding sites in neuronal tissue. | the phylogenetic distribution of specific binding sites for kainic acid was determined in 14 species including invertebrates and vertebrates. the highest level of binding was observed in brains of the frog (xenopus laevis), followed by the spiny dogfish (heterodontus francisci), the goldfish (carasius auratus) and the chick (gallus domesticus). although significant specific binding was noted in some of the lowest forms tested (e.g. hydra littoralis), this was not a consistent observation in the ... | 1980 | 6247025 |
| controlled release effect of insecticide microcapsules and their results in common household insect pest control. | since 1978 we have prepared microencapsulated insecticides by complex coacervation and interfacial polymerization for household insect pest control, fenthion being the main core element. a series of tests have been conducted against mosquitoes (culex pipiens quinquefasciatus), cockroaches (periplaneta americana) and bedbugs (cimex lectularius), and the results indicate that microencapsulation can prolong persistent effects by 2-12 times, enhance safety and subdue offensive odour. furthermore, th ... | 1984 | 6336530 |
| immunological recognition of cuticular transplants in insects. | transplantation of allogeneic and xenogeneic cuticle onto the american cockroach, periplaneta americana, was carried out in order to compare the specificity of immune recognition of these 'skin grafts' with that of implanted tissues. in order to facilitate interpretation of results, the technique of transplanting cuticle from nymphal donors onto nymphal recipients was adopted - if donor subcuticular epidermis is not recognised as 'foreign', it will grow, fuse with the recipient's epidermal sheet ... | 1983 | 6341106 |
| laboratory studies on the susceptibility of blattella germanica and periplaneta americana to bacillus thuringiensis var. israelensis. | | 1984 | 6523178 |
| isolation and characterization of two myoactive neuropeptides: further evidence of an invertebrate peptide family. | the neuropeptide proctolin acts as a neuromuscular co-transmitter in insect skeletal muscle. as a prelude to determining whether other peptides may function in a similar way, we are attempting to isolate and characterize the chemical nature of new myoactive neuropeptides in insects. we examined the corpus cardiacum, a major insect neurosecretory structure of the american cockroach (periplaneta americana), using a skeletal muscle bioassay and high pressure liquid chromatography fractionation and ... | 1984 | 6699686 |
| localization of pancreatic polypeptide (pp)-like immunoreactivity in the central and visceral nervous systems of the cockroach periplaneta. | the central and visceral nervous systems of the cockroach periplaneta americana were studied by means of the peroxidase-antiperoxidase immunocytochemical method, with the use of antibody to bovine pancreatic polypeptide (pp). pp-like immunoreactive neuron somata are most numerous in the brain; at least 6 pairs of cell groups occur in clearly defined regions. three pairs of cells each are also present in the suboesophageal ganglion and the thoracic ganglia, one pair of single cell each in the fir ... | 1982 | 6756638 |
| insect erythrocyte agglutinins. in vitro opsonization experiments with clitumnus extradentatus and periplaneta americana haemocytes. | the effect of naturally occurring haemagglutinins on the in vitro phagocytosis of sheep erythrocytes by the blood cells (haemocytes) of clitumnus extradentatus and periplaneta americana was studied. the results showed that the haemagglutinins in both species failed to act as opsonins. indeed, in some instances, incubation of erythrocytes in haemolymph resulted in less avid ingestion as compared with the saline-incubated controls. this reduced phagocytosis was probably caused by the clumping of e ... | 1980 | 7000682 |
| immunocytochemical identification of neural elements in the central nervous systems of a snail, some insects, a fish, and a mammal with an antiserum to the molluscan cardio-excitatory tetrapeptide fmrf-amide. | with an antiserum to the molluscan cardio-excitatory tetrapeptide fmrf-amide neurons and/or nerve fibers were immunocytochemically identified in the central nervous systems of a snail (lymnaea stagnalis), some insects (leptinotarsa decemlineata, periplaneta americana, locusta migratoria, pieris brassicae), a fish (poecilia latipinna) and a mammal (mouse). the fact that immunoreactive material was observed in neurohaemal organs (corpora cardiaca of the insects) as well as in axon terminals ending ... | 1980 | 7006829 |
| morphogenesis of the proboscis hooks of an archiacanthocephalan, moniliformis moniliformis (bremser 1811) travassos 1915. | proboscis hooks of moniliformis moniliformis were noted first as small protrusions on developing proboscides of 20- to 28-day-old acanthellae from hemocoels of experimentally infected cockroaches (periplaneta americana). on the basis of proximity, residual connecting strands of tissue, and ultrastructural similarity, origin of the hooks has been determined to be from primordial muscle tissue. during development, primordial proboscis hooks extended into hypodermal tissue, but remained separated b ... | 1980 | 7218121 |
| humoral mechanisms in the immune response of insects to larvae of hymenolepis diminuta (cestoda). | previous work has shown that larvae of hymenolepis diminuta are not encapsulated by the haemocytes of the locust, schistocerca gregaria, but are encapsulated by haemocytes of the cockroach, periplaneta americana. the specificity of the cellular immune response in vivo, and of the agglutinating activity of the sera against vertebrate erythrocytes in vitro, is known to differ between the two species of insect. accordingly, the sera of each of these species was tested for its agglutinating activity ... | 1981 | 7301407 |
| prevalence of allergy to some inhalants among rhinitis patients in malaysia. | this study was conducted to determine the seasonal prevalence of allergies to house dust, d. pteronyssinus, d. farinae, cat fur, dog hair, mixed moulds, mixed grass pollens and american cockroach. a total of 314 patients with clinically suspected allergic rhinitis was examined by prick test using commercial preparations of the above allergens. total serum ige of the patients was determined by a sandwich elisa. ninety-six percent of the patients tested positive to more than one allergen. most wer ... | 1995 | 7488338 |
| isolation and preliminary characterization of cdna encoding american cockroach allergens. | two prominent proteins of 78 and 72 kd in cr-pi have been found to be the major allergens of american cockroach (periplaneta americana). | 1995 | 7560637 |
| actions of neurotoxins (bungarotoxins, neosurugatoxin and lophotoxins) on insect and nematode nicotinic acetylcholine receptors. | neurotoxins of natural origin have proved to be of considerable value in the isolation and characterization of vertebrate muscle and neuronal nicotinic acetylcholine receptors (nachrs). to date, they have been used less extensively in studies of invertebrate nachrs. here we examine how a variety of neurotoxins (the snake toxins alpha-bungarotoxin, alpha-bgt, and kappa-bungarotoxin, kappa-bgt, the molluscan toxin, neosurugatoxin, and the soft coral toxins, lophotoxin and bipinnatin-b) can be used ... | 1995 | 7570627 |
| biologic potency and immunoblotting studies of extracts of three cockroach species. | although cockroaches are responsible for severe allergic disease, cockroach extracts are not yet standardized by the id50eal method, nor have the allergens been completely characterized. | 1995 | 7583846 |
| localization of octopaminergic neurones in insects. | this paper reviews data on the localization of octopaminergic neurons revealed by immunocytochemistry in insects, primarily the locusts schistocerca gregaria and locusta migratoria, cricket gryllus bimaculatus, and cockroach periplaneta americana. supporting evidence for their octopaminergic nature is mentioned where available. in orthopteran ventral ganglia, the major classes of octopamine-like immunoreactive (-li) neurones include: (1) efferent dorsal and ventral unpaired median (dum, vum) neu ... | 1995 | 7712064 |
| peptides related to the diploptera punctata allatostatins in nonarthropod invertebrates: an immunocytochemical survey. | the allatostatins are a family of peptides isolated originally from the cockroach, diploptera punctata. related peptides have been identified in periplaneta americana and the blowfly, calliphora vomitoria. these peptides have been shown to be potent inhibitors of juvenile hormone synthesis in these species. a peptide inhibitor of juvenile hormone biosynthesis has also been isolated from the moth, manduca sexta; however, this peptide has no structural homology with the d. punctata-type allatostat ... | 1994 | 7822491 |
| cross reactivity studies of crf-related peptides on insect malpighian tubules. | manduca sexta diuretic peptide ii (mas-dpii) stimulates fluid secretion by adult malpighian tubules and cyclic amp production by larval proximal and adult tubules of m. sexta in a dose-dependent manner. mas-dpii has no effect on fluid transport across the larval cryptonephric complex. m. sexta diuretic hormone (mas-dh) and crf-related insect diuretic peptides from acheta domesticus, locusta migratoria, and periplaneta americana also cause similar increases in the production of cyclic amp by the ... | 1995 | 7866779 |
| the detection of akh/hrth-like peptides in ascaridia galli and ascaris suum using an insect hyperglycaemic bioassay. | evidence for the presence of adipokinetic hormone/hypertrehalosaemic hormone (akh/hrth)-like peptides in the parasitic nematodes ascaridia galli and ascaris suum has been obtained using insect bioassays which measure hyperglycaemic responses to peptides belonging to the akh/hrth family of insect hormones. a peptide fraction extracted from heads and tails of ascaridia galli evoked a dose-dependent hyperglycaemic response when injected into the cockroach, periplaneta americana. maximal bioactivity ... | 1994 | 8008462 |
| [hyp3]met-callatostatin. identification and biological properties of a novel neuropeptide from the blowfly calliphora vomitoria. | a novel, hydroxyproline-containing neuropeptide, gly-pro-hyp-tyr-asp-phe-gly-met-nh2, designated [hyp3]met-callatostatin, has been identified from extracts of heads of the blowfly calliphora vomitoria. the peptide is a naturally occurring hydroxylate analogue of met-callatostatin, a previously identified allatostatin-like peptide, and is present to the extent of 20% of the nonhydroxylated form. in bioassays, both forms of the peptide show allatostatic activity by inhibiting juvenile hormone synt ... | 1994 | 8063725 |
| octopamine enhances phagocytosis in cockroach hemocytes: involvement of inositol trisphosphate. | octopamine and 5-hydroxytryptamine (5-ht) were previously shown to affect phagocytosis in cockroach hemocytes through unidentified receptor-mediated events. in the present study, we examined the ability of 5-ht and octopamine to enhance inositol trisphosphate (ip3) production using hemocyte membranes of the american cockroach, periplaneta americana. octopamine enhanced ip3 production with a maximal peak at 100 nm. similarly, 5-ht enhanced ip3 production with a maximal effect at 10 nm. the effect ... | 1994 | 8068962 |
| evolution of adaptive immunity: inducible responses in the american cockroach. | | 1994 | 8192355 |
| purification and characterization of vitellin-2 from the ovary of the american cockroach, periplaneta americana. | two vitellins (vn-1 and vn-2) were identified in cockroach ovary. vn-2 was purified by ion-exchange chromatography using deae cellulose and sepharose cl-6b, and gel filtration using sephadex g-200 and sepharose 4b. vn-2 comprises three subunits with molecular weights of 102, 72 and 40 kda. vn-2 contains diacylglycerol and triacylglycerol; their fatty acids are mostly lauric acid and palmitoleic acid. vn-2 also has phosphatidylcholine and phosphatidylethanolamine but no sphingomyelin. vn-2 contai ... | 1994 | 8205388 |
| localization of corazonin in the nervous system of the cockroach periplaneta americana. | antisera raised to the cardioactive peptide corazonin were used to localize immunoreactive cells in the nervous system of the american cockroach. sera obtained after the seventh booster injection were sufficiently specific to be used for immunocytology. they recognized a subset of 10 lateral neurosecretory cells in the protocerebrum that project to, and arborize and terminate in the ipsilateral corpus cardiacum. they also reacted with bilateral neurons in each of the thoracic and abdominal neuro ... | 1993 | 8242711 |
| toxicity of the venom from nasonia vitripennis (hymenoptera: pteromalidae) toward fly hosts, nontarget insects, different developmental stages, and cultured insect cells. | a venom preparation from nasonia vitripennis, a wasp ectoparasitoid of fly pupae, was assayed for lethality in different stages of insects representing ten different orders and in cultured insect cells. in most cases, the motor activity of the injected insects remained completely normal for 1-2 days after the injection and displayed none of the symptoms of paralysis commonly reported for venoms of the hymenoptera. a natural host, the flesh fly sarcophaga bullata, was highly sensitive in the pupa ... | 1993 | 8342173 |
| isolation and in vitro translation of messenger rna from the american cockroach. | total rna was extracted from the whole body of american cockroach (periplaneta americana) using chaotropic salt guanidine isothiocyanate in the presence of 2-mercaptoethanol (2-me). polyadenylated mrna was isolated by oligothymidylic acid-cellulose chromatography and mrna was translated using a rabbit reticulocyte lysate system. the translation, as judged by the incorporation of 35s-methionine, was obtained with poly(a)+ rna, where an approximately 9.5-fold increase in label incorporation over c ... | 1993 | 8369976 |
| transplantation immunity in the american cockroach, periplaneta americana: the rejection of integumentary grafts from blatta orientalis. | the results from several previous studies have indicated that the american cockroach is able to respond to integumentary xenografts, but doubt remained as to whether cockroaches could effectively discriminate between self and allogeneic differences. this was emphasized by the fact that while one study reported that periplaneta americana responded to grafts donated by the closely related genus blatta orientalis, the data reported elsewhere, using a different assay system, found no such reactivity ... | 1993 | 8375565 |
| lipid transfer particle in the hemolymph of the american cockroach: evidence for its capacity to transfer hydrocarbons between lipophorin particles. | a lipid transfer particle (ltp) was isolated and purified from the hemolymph of the adult male american cockroach, periplaneta americana, essentially according to the method previously developed for the purification of ltp from locust hemolymph. fast protein liquid chromatography (fplc) on a mono q column was used as the additional step to obtain pure ltp. the electron micrograph of purified cockroach ltp exhibited an unusual and asymmetric shape essentially similar to that reported for manduca ... | 1993 | 8496660 |
| quality of housing and allergy to cockroaches in the dominican republic. | fifty-one atopic asthmatic and/or allergic rhinitic children and 23 nonatopic control from santo domingo, the dominican republic, were skin tested with an extract mix of three cockroach species (blattella germanica, blatta orientalis, and periplaneta americana). sixteen percent of the atopics and none of the nonatopics demonstrated positive immediate skin reactions to the cockroach mix (chi 2 = 4.05, p = 0.04). hypersensitivity was correlated with the quality of the homes; 22% (8/36) of the atop ... | 1996 | 8527953 |
| cloning of cockroach allergen, bla g 4, identifies ligand binding proteins (or calycins) as a cause of ige antibody responses. | an allergen cloned from a blattella germanica (german cockroach) cdna library, encoded a 182-amino acid protein of 20,904 da. this protein, designated b. germanica allergen 4 (bla g 4), was expressed as a glutathione s-transferase fusion protein in escherichia coli and purified by affinity chromatography and high-performance liquid chromatography. the prevalence of serum ige antibody to recombinant bla g 4 in 73 cockroach allergic patients with asthma ranged from 40% (antigen binding radioimmuno ... | 1995 | 8537384 |
| identification of low molecular weight allergens of american cockroach and production of monoclonal antibodies. | previous studies have identified two allergic fractions (cr-pi and cr-pii) from crude american cockroach extract, from which the 72- and 78-kilodalton (kd) components of cr-pi have been identified as major allergens. | 1996 | 8595541 |
| serum total and specific ige levels in children infected with human immunodeficiency virus. | human immunodeficiency virus (hiv) infection is associated with polyclonal b-cell activation, and elevated ige levels are found in infected adults. chronic nasal symptoms, sinusitis, and otitis media are particularly common in children infected with hiv. the possible allergic cause of nasal symptoms in children infected with hiv has not been explored. | 1996 | 8621856 |
| a comparative immunocytochemical study using an antiserum against a synthetic analogue of the corpora cardiaca peptide pea-cah-i (mi, neurohormone d) of periplaneta americana. | an antiserum against the octapeptide pea-cah-i, a member of the adipokinetic hormone/red pigment-concentrating hormone family, has been produced for immunocytochemical staining in insects and various other invertebrate species. the anti-pea-cah-i serum stains the glandular corpora cardiaca cells of those insect species that synthesize identical or structurally similar peptides. in the corpora cardiaca of species producing peptides with a different c-terminus, these cells remain unstained. pea-ca ... | 1996 | 8646760 |
| comparative performance for immediate hypersensitivity skin testing using two skin prick test devices. | fifty-five patients were skin-tested by multi-test (m) and needle prick test (npt) to compare the reproducibility of the methods. we used 6 allergenic extracts: dermatophagoides pteronyssinus, dermatophagoides farinae, dog epithelium, cat pelt, american cockroach and mixed molds. a glycerosaline and a positive control (histamine 1 mg/ml) were performed in both methods. statistically significant differences in histamine and dermatophagoides farinae wheal reactions between the two methods (m > npt ... | 1995 | 8653226 |
| sequencing analysis of cdna clones encoding the american cockroach cr-pi allergens. homology with insect hemolymph proteins. | a previous article described the isolation of several lambdagt22a cdna clones expressing the american cockroach (periplaneta americana) cr-pi allergens recognized by both human atopic ige antibodies and anti-cr-pi monoclonal antibodies (wu, c. h., lee, m. f., and liao, s. c.(1995) j. allergy clin. immunol. 96, 352-359). this article presents the nucleotide and deduced amino acid sequences of two cdna clones encoding major allergens of p. americana. clones c12 and c20 encode proteins of 685 and 6 ... | 1996 | 8663281 |
| a recombinant insect-specific alpha-toxin of buthus occitanus tunetanus scorpion confers protection against homologous mammal toxins. | we have constructed a cdna library from venom glands of the scorpion buthus occitanus tunetanus and cloned a dna sequence that encodes an alpha-toxin. this clone was efficiently expressed in escherichia coli as a fusion protein with two ig-binding (z) domains of protein a from staphylococcus aureus. after cnbr treatment of the fusion protein and hplc purification, we obtained approximately 1 mg recombinant apha-toxin/l bacterial culture. the toxin, called bot xiv, displays no toxicity towards ma ... | 1996 | 8706664 |
| [environmental pollution and infectious diseases. i. isolation of a new serovar of the salmonella genus, s.v 13.22:r: from a periplaneta americana cockroach]. | the aa have isolated a new serovar of genus salmonella s.v 13.22:r:-, from a cockroach periplaneta americana, captured near the municipal slaughterhouse of messina and near the faculty of veterinary science. this fifth original report fift in the vast subject of the environmental pollution, where the cockroaches, as widely documented, unwind a primary importance part as spreader of salmonella, too. | 1994 | 8706968 |
| [characteristics of a new salmonella serovar: s. v 13, 22:r-, from s. bongori species]. | the a reports the characteristics of a new serovar of salmonella: s.v 13, 22:r:-, isolated from a cockroach periplaneta americana, all set against the background of ample technic notes. | 1994 | 8706970 |
| studies of allergen extract stability: the effects of dilution and mixing. | however potent the allergy extracts provided by manufacturers, they are subject to deterioration with storage, especially after dilution or mixture with other extracts. | 1996 | 8757215 |
| host specificity of gregarina blattarum von siebold, 1839 (apicomplexa: eugregarinida) among five species of domiciliary cockroaches | the host specificity of gregarina blattarum was evaluated among five species of domiciliary cockroaches: blatella germanica, supella longipalpa, blatta orientalis, periplaneta americana, and periplaneta fuliginosa. third- and fourth-instar nymphs were allowed to feed on crushed dog kibble contaminated with g. blattarum oocysts. cockroaches were killed 8 days postinfection and examined for parasites. gregarina blattarum infections were observed in all experimentally infected replications of b. ge ... | 1996 | 8812602 |
| presence of the periplaneta lectin-related protein family in the american cockroach periplaneta americana. | we determined the partial amino acid sequences of periplaneta lectin, which we had purified and characterized previously from the hemolymph of the american cockroach (periplaneta americana) [kubo t. and natori s. (1987) eur. j. biochem. 168, 75-82]. based on these sequences, we performed pcr and found that the cdna library of the periplaneta fat body contained many similar, but not identical, periplaneta lectin-related cdnas. analysis of the cloned cdnas suggested that periplaneta has a protein ... | 1996 | 8814782 |
| pharmacological induction of rhythmical activity and plateau action potentials in unmyelinated axons. | the physiological function of the axon is to conduct short all-or-none action potentials from their site of initiation (usually the cell body) to the synapse. to ensure this function, both passive and active biophysical properties of the axons are tuned very precisely, especially the voltage-dependent ionic conductances to sodium and potassium. under normal conditions, axons are not spontaneously active. minor modifications of their ionic micro-environment or slight changes in the membrane prope ... | 1995 | 8861816 |
| allergy to cockroaches in patients with asthma and rhinitis in an urban area (madrid). | previous studies have established that cockroach allergens are important sensitizing agents in the induction of rhinitis and asthma principally in urban areas. this study was undertaken to assess skin test reactivity and specific ige antibody reactivity to extracts of periplaneta americana (p.a.), blattella germanica (b.g.), b. orientalis (b.o.), and a fecal extract of b. germanica (feb.g.) in a group of patients with rhinitis and asthma living in an urban area in europe. we examined clinical ch ... | 1996 | 8874663 |
| cross reactivity between the antigens of periplaneta americana and triatoma infestans. | the cockroach periplaneta americana (pa) and the reduviid triatoma infestans (ti) are two very common insects in argentina that pollute indoor and outdoor environments. they are also strongly immunogenic in animals and in atopic men. several immunological methods such as serological absorption procedures, diffusion gel methods and capillary electrophoresis were applied in order to establish the existence of common glycoprotein epitopes in the cockroach and in the reduviid. the presence of specif ... | 1996 | 8959541 |
| diflubenzuron stimulates phosphorylation of a 39 kda integumental protein from newly molted american cockroach (periplaneta americana). | we identified a 39 kilodalton (kda) protein from newly molted american cockroaches (periplaneta americana), isolated from a 100,000 g precipitate of a 1000 g supernatant from an integument homogenate, which exhibited increased phosphorylation following diflubenzuron (dfb) treatment. this 39 kda phosphoprotein was partially purified from an intracellular membrane vesicle containing fractions obtained by discontinuous sucrose density centrifugation. both the interfaces of the 30/40% and 40/50% suc ... | 1996 | 9014334 |
| allergen skin test reactivities among asthmatic thai children. | skin prick tests with 14 selected local aeroallergens were performed on 100 asthmatic children aged 0-16 years attending the pediatric allergy clinic, faculty of medicine, siriraj hospital. the 14 allergens included dermatophagoides farinae, dermatophagoides pteronyssinus, american cockroach (periplaneta americana), johnson grass (sorghum halopense), cladosporium clarosporoides, alternaria tenuis, penicillium mix, aspergillus mix, cat dander, dog dander, milk, egg white, soy and shrimp. positive ... | 1997 | 9078689 |
| evaluating the factors that relate to asthma severity in adolescents. | over the past 5 years, we have been engaged in a cross-sectional evaluation of risk factors for higher asthma severity in adolescents aged 13-18. all recruitment takes place through public and private schools. the sample from which our current findings are derived includes 151 adolescents covering a wide spectrum of asthma severity and socioeconomic status (ses) and representing both african american and caucasians. an asthma severity instrument has been developed and validated for the purpose o ... | 1997 | 9130491 |
| false positive immunostaining of locusta neurosecretory cells with a variety of preimmune sera. | a large number of antisera directed against vertebrate neuropeptides have been reported to yield positive staining when applied to insect brains. in most cases, the preimmune serum of the same animal in which the antiserum was developed is not available for testing in control experiments. we have experienced that a large percentage of preimmune sera, as well as a culture medium for hybridomas, stain cell populations and fibers in the central nervous system of the insect locusta migratoria. purif ... | 1997 | 9169119 |
| analysis of cross-reactive allergens from american and german cockroaches by human ige. | serum from atopics hypersensitive to the american cockroach were examined for their specific ige to american and german cockroaches by the fluoroallergosorbent test (fast). of 44 sera tested, 86.4% (38/44) contained ige to american and german cockroaches, and 13.6% (6/44) were found to be positive for american cockroach alone by fast. nine individual sera containing ige antibodies to both cockroaches were used to analyze the cross-reacting allergens in the crude american and german cockroach ext ... | 1997 | 9188922 |
| distribution of sulfakinin-like peptides in the central and sympathetic nervous system of the american cockroach, periplaneta americana (l.) and the field cricket, teleogryllus commodus (walker). | we describe the distribution of sulfakinin-like neuropeptides in the central and sympathetic nervous system of the american cockroach periplaneta americana (l.) (blattodea) and the field cricket teleogryllus commodus (walker) (othoptera), using an antisulfakinin primary antibody and confocal laser scanning microscopy. we conclude that, in the cockroach, sulfakinin-like material is produced in ten pairs of anterior cells in the pars intercerebralis, as well as two pairs of medial and one major pa ... | 1997 | 9225486 |
| effects of [3h]-bidn, a novel bicyclic dinitrile radioligand for gaba-gated chloride channels of insects and vertebrates. | 1. the radiolabelled bicyclic dinitrile, [3h]-3,3-bis-trifluoromethyl-bicyclo[2.2.1]heptane-2,2-dicarbonitrile ([3h]-bidn), exhibited, specific binding of high affinity to membranes of the southern corn rootworm (diabrotica undecimpunctata howardi) and other insects. a variety of gamma-aminobutyric acid (gaba) receptor convulsants, including the insecticides heptachlor (ic50, 35 +/- 3 nm) and dieldrin (ic50, 93 +/- 7 nm), displaced [3h]-bidn from rootworm membranes. when tested at 100 microm, 1 ... | 1997 | 9257933 |
| progesterone in periplaneta americana and neobellieria bullata adults from the procuticle phase until first progeny production. | a significant amount of progesterone-like immunoreactive material (150 ng/g) was measured by eia in the procuticle phase of adult of both sexes of periplaneta americana. this peak markedly decreased to 1-10 ng/g during sclerotization and was unlikely to be of dietary origin. in the case of 0-hr-old p. americana adults 96-98% of progesterone-like material was localized in the digestive tract and malpighian tubules. in contrast, a relatively low level of progesterone-like immunoreactive material w ... | 1997 | 9268626 |
| cockroaches from urban human dwellings: isolation of bacterial pathogens and control. | a study was carried out to determine the distribution of cockroaches in two different housing areas with central sewerage or individual septic tanks in an urban area in kuala lumpur, malaysia. six species of cockroaches were present and of these periplaneta americana and periplaneta brunnea were found in greater abundance. seventeen species of bacteria were isolated and of these escherichia coli and klebsiella p. pneumoniae were isolated in greatest numbers. control measures carried out using la ... | 1997 | 9322309 |
| central action of insect neuropeptide, periplanetin cc-1, in mice. | the central action of periplanetin cc-1 (pea-hrth) (glp-val-asn-phe-ser-pro-asn-trpnh2), octapeptide of the insect adipokinetic hormone family (akh-family), isolated from american cockroach-periplaneta americana, was studied in albino swiss mice (20-25 g). cc-1 was injected intracerebroventricularly (i.c.v.) in the volume of 5 microliters at the dose of 50 ng/mouse. it was found that cc-1 showed strong analgesic activity in "writhing syndrome" test and in "hot plate" test. in addition, periplane ... | 1997 | 9431551 |
| bidn, a bicyclic dinitrile convulsant, selectively blocks gaba-gated cl- channels. | bidn (3,3-bis(trifluoromethyl)bicyclo[2,2,1]heptane-2,2-dicarbonitrile) at 10(-5) m blocked gaba-induced inhibitory postsynaptic potentials (ipsps) recorded from an identified, giant interneuron (g12) of the cockroach (periplaneta americana). the same concentration of this bicyclic dinitrile also blocked cl- -mediated responses of g12 to gaba applied by pressure microinjection into the terminal abdominal ganglion neuropile containing dendrites of g12. bidn (10(-5) m) was without effect on a resp ... | 1998 | 9473568 |
| niche overlap among three species of pinworm parasitic in the hindgut of the american cockroach, periplaneta americana. | individual american cockroaches, periplaneta americana, often harbor 3 species of pinworm (oxyurida: nematoda) thelastoma bulhoesi, leidynema appendiculatum, and hammerschmidtiella diesingi, simultaneously. we looked for evidence of trophic niche segregation based on differences in size preference among the 3 species. fluorescent beads of 1, 3, 6, and 10 microm diameter were mixed in agar and fed to individual hosts. adult female worms of each species were removed from hosts, and the number of e ... | 1998 | 9576494 |
| aeroallergen sensitivity of thai patients with allergic rhinitis. | the aim of this study was to determine the aeroallergen sensitivity of allergic rhinitis patients. a total of 100 cases (female: 59, male: 41, aged between 10-59 years, mean age 27.9 years) who were diagnosed with allergic rhinitis by history and clinical presentation, underwent a prick skin test with 30 aeroallergens, and the important sensitizing allergens were assessed. skin test reactivity showing > or = 3 mm wheal with erythema as the positive skin test, was recorded. the results of patient ... | 1997 | 9579610 |
| prevalence of skin reactions to aeroallergens in asthmatics of puerto rico. | in puerto rico, although a high prevalence of asthma has been reported, the sensitization rates to aeroallergens in these patients is unknown. the purpose of this study using a case control design, was to determine and compare the rates of sensitization to common aeroallergens in an asthmatic population of 576 asthmatics and 144 healthy controls. a skin prick test was conducted using standardized extracts of dermatophagoides farinae (df) and d. pteronyssinus (dp), house dust (hd), cat hair and e ... | 1997 | 9580406 |
| cloning of the american cockroach cr-pii allergens: evidence for the existence of cross-reactive allergens between species. | previously, we have identified the 28 and 32 kd proteins as additional important allergens from the american cockroach (periplaneta americana) cr-pii allergenic fraction. | 1998 | 9648712 |
| use of random amplified polymorphic dna (rapd) for generating specific dna probes for oxyuroid species (nematoda). | random amplified dna markers (rapd; williams et al., 1990) were used to obtained specific rapd fragments characterising different species of oxyuroids. we tested six species of worms parasitizing vertebrates or invertebrates: passalurus ambiguus rudolphi, 1819, parasite of leporids; syphacia obvelata (rudolphi, 1802) seurat, 1916, a parasite of rodents; blatticola blattae (graeffe, 1860) chitwood, 1932 parasite of the cockroach blattella germanica; hammerschmidtiella diesingi (hammerschmidt, 183 ... | 1998 | 9754296 |
| genetic influences of chromosomes 5q31-q33 and 11q13 on specific ige responsiveness to common inhaled allergens among african american families. collaborative study on the genetics of asthma (csga). | we have recently conducted a genome-wide screening for genes influencing dermatophagoides pteronyssinus-specific ige responsiveness as a part of the collaborative study on the genetics of asthma (csga), which showed evidence for linkage in some regions, including chromosomes 5131-q33 and 11q13 in african american families. | 1998 | 9768587 |
| sensitization to cockroach allergens in a sample from the urban population living in naples (southern italy). | the aim of this study was to evaluate the prevalence of allergic sensitization to cockroach allergens in a group of atopic patients living in the urban area of naples. four hundred and fourteen consecutive patients were submitted to the following diagnostic procedures: anamnestic data; clinical examination; and skin prick test by using a standardized pricker and allergenic extracts, one of which contained a mix of whole bodies of blattella orientatis, blattella germanica and periplaneta american ... | 1998 | 9777540 |
| novel allergen structures with tandem amino acid repeats derived from german and american cockroach. | cockroaches produce potent allergens that are an important cause of asthma. the two principal domiciliary cockroach species, blattella germanica and periplaneta americana, secrete major allergens, bla g 1 and per a 1. here, we report the molecular cloning of three bla g 1 cdna clones, which showed 70% amino acid sequence identity with per a 1. plaque immunoassays with human ige antibodies or murine monoclonal antibodies showed that these allergens were antigenically cross-reactive. the bla g 1 s ... | 1998 | 9804858 |
| sensitization to mite allergens and acute asthma in a tropical environment. | the objective of this study was to determine whether sensitization to certain aeroallergens is associated with acute asthma in cartagena, colombia. ninety-nine subjects with acute asthma and 100 controls evaluated for other reasons were studied at a local emergency room. sensitization was established by specific ige determinations. statistically significant differences between asthmatic subjects and controls were found in the prevalence of specific ige to dermatophagoides pteronyssinus, 64.6% vs ... | 1998 | 9827423 |
| mushroom bodies of the cockroach: their participation in place memory. | insects and other arthropods use visual landmarks to remember the location of their nest, or its equivalent. however, so far, only olfactory learning and memory have been claimed to be mediated by any particular brain region, notably the mushroom bodies. here we describe the results of experiments that demonstrate that the mushroom bodies of the cockroach (periplaneta americana), already shown to be involved in multimodal sensory processing, play a crucial role in place memory. behavioral tests, ... | 1998 | 9862324 |
| ionic mechanisms underlying depolarizing responses of an identified insect motor neuron to short periods of hypoxia. | hypoxia can dramatically disrupt neural processing because energy-dependent homeostatic mechanisms are necessary to support normal neuronal function. in a human context, the long-term effects of such disruption may become all too apparent after a "stroke," in which blood-flow to part of the brain is compromised. we used an insect preparation to investigate the effects of hypoxia on neuron membrane properties. the preparation is particularly suitable for such studies because insects respond rapid ... | 1999 | 9914291 |
| two subunits of the insect 26/29-kda proteinase are probably derived from a common precursor protein. | we previously identified the 26/29-kda proteinase in the hemocytes of sarcophaga peregrina (flesh fly) that appears to participate in elimination of foreign proteins in this insect [eur. j. biochem. 209, 939-944 (1992)]. here, we report the cdna cloning of this proteinase. the cdna encodes a protein which includes both the 26- and 29-kda subunit, strongly suggesting that the both subunits are derived from a single precursor protein. the 26- and 29-kda subunit located at the amino-terminal and ca ... | 1999 | 10050046 |
| the molecular evolution of the allatostatin precursor in cockroaches. | allatostatins (asts) of the tyr/phe-xaa-phe-gly leu/ile-nh2 family are a group of insect neuropeptides that inhibit juvenile hormone biosynthesis by the corpora allata. we have obtained genomic dna sequences that specify the preproallatostatin precursor for the cockroaches, blatta orientalis, blattella germanica, blaberus (cranufer and supella longipalpa. the sequences obtained are similar to those of diploptera punctata and periplaneta americana reported previously. the precursors of all these ... | 1999 | 10098619 |
| comparison of rates of penetration through insect cuticle of amphiphylic analogs of insect pyrokinin neuropeptides. | rates of penetration through the cuticle of amphiphylic analogs, synthesized by addition of 6-phenylhexanoic acid or 9-fluoreneacetic acid or 1-pyrenebutyric acid to the amino terminus of the pentapeptide phe-thr-pro-arg-leu-amide, were assessed by quantitative analysis using reversed phase liquid chromatography. the analogs effectively penetrated the cuticle of both the adult american cockroach and tobacco budworm moth. however, the amounts of analogs that penetrated the cuticle of the cockroac ... | 1999 | 10098625 |
| molecular characterization of american cockroach tropomyosin (periplaneta americana allergen 7), a cross-reactive allergen. | inhalation of allergens produced by the american cockroach (periplaneta americana) induces ige ab production and the development of asthma in genetically predisposed individuals. the cloning and expression in escherichia coli of p. americana tropomyosin allergen have been achieved. the protein shares high homology with other arthropod tropomyosins (80% identity) but less homology with vertebrate ones (50% identity). the recombinant allergen was produced in e. coli as a nonfusion protein with a y ... | 1999 | 10201967 |
| immunologic characterization of a recombinant american cockroach (periplaneta americana) per a 1 (cr-pii) allergen. | previously, we have identified several per a 1 (cr-pii) allergens from a deltagt22a cdna library of periplaneta americana. this study aimed to sequence clone c42 and determine its molecular and antigenic properties. | 1999 | 10221434 |
| molecular cloning of per a 1 and definition of the cross-reactive group 1 cockroach allergens. | sensitization to allergens produced by german and american cockroaches is strongly associated with the cause of asthma. most of the cockroach allergens identified to date have been species specific. | 1999 | 10329820 |
| immulectin, an inducible c-type lectin from an insect, manduca sexta, stimulates activation of plasma prophenol oxidase. | immulectin, a c-type lectin from the tobacco hornworm, manduca sexta, was cloned from a larval fat body cdna library. the immulectin cdna encodes a 309 residue polypeptide. immulectin synthesis was induced by injection of killed gram-positive or gram-negative bacteria or yeast. after injection of bacteria, immulectin mrna appeared in fat body and immulectin protein was detected in hemolymph. immulectin contains two carbohydrate recognition domains. the carboxyl-terminal carbohydrate recognition ... | 1999 | 10436935 |
| cockroach allergens and asthma in brazil: identification of tropomyosin as a major allergen with potential cross-reactivity with mite and shrimp allergens. | cockroaches produce several proteins that induce ige antibody responses. although cockroaches are abundant in warm and humid areas, sensitization to cockroach allergens has not been investigated in brazil. | 1999 | 10452753 |
| proteinase and gelatinolytic activities of house dust mite and cockroach extracts. | the presence of gelatinolytic activity in dust mite and periplaneta americana allergenic crude extracts were studied. the former presented major activity in a broad band between 45 and 66 kda and minor activity at 32 kda, while the latter showed a more complex pattern with gelatinolytic activity at 90, 78, 65, 34, 32 and 24 kda. when the proteolytic activity patterns of dust mites and cockroach crude extracts were analyzed at three different ph levels, the proteases in both cases were optimally ... | 1999 | 10513350 |
| antisera against periplaneta americana cu,zn-superoxide dismutase (sod): separation of the neurohormone bursicon from sod, and immunodetection of sod in the central nervous system. | in an effort to characterize the insect molting hormone bursicon from the cockroach, periplaneta americana, amino acid sequences with high identity of cu,zn-superoxide dismutase (sod) of drosophila virilis were identified. antisera against a conserved region of sod, and a sequence unique to periplaneta sod were produced and used to test whether bursicon might be a form of sod. western blots of one- and two-dimensional gels revealed that the dimeric form of sod and bursicon have a similar molecul ... | 1999 | 10528407 |
| sensitization to local dust-mite fauna in singapore. | recent studies showed the presence of a unique dust-mite fauna in the indoor environment of singapore. immediate hypersensitivity to these dust mites, along with other known indoor allergens, may play a role in the pathogenesis of allergic respiratory diseases. this study evaluated the sensitization rates of the local atopic population to these allergens. | 1999 | 10604550 |
| comparative insecticide susceptibility and detoxification enzyme activities among pestiferous blattodea. | topical bioassays using propoxur, chlorpyrifos, and lambda-cyhalothrin were conducted on eight cockroach species. based on lethal dose values, the relative toxicities of the insecticide classes were generally pyrethroid > carbamate > organophosphorous. lambda-cyhalothrin and propoxur were more toxic toward the blattidae as compared with the blattellidae. the order of lambda-cyhalothrin toxicity was periplaneta americana > periplaneta brunnea = periplaneta australasiae = periplaneta fuliginosa = ... | 1999 | 10661713 |
| neural electrophysiological effect of crude venom of conus textile from the south china sea. | in the present study, we characterized effects of the crude venom from conus textile, a marine molluscivorous snail collected from the south china sea, on neural electrophysiological activity in insect, molluscan and mammalian species. our results demonstrate that the venom reversibly blocks the cholinergic synaptic transmission of cockroach periplaneta americana central nervous system, partially blocks na(+) currents in rat hippocampal ca(1) pyramidal neurons, and enhances the excitability and ... | 2000 | 10775760 |
| the interactions between juvenile hormone (jh), lipophorin, vitellogenin, and jh esterases in two cockroach species. | for the cockroach species leucophaea maderae and periplaneta americana two major juvenile hormone (jh)-binding proteins have been identified: lipophorin (lp) and vitellogenin (vg). each of these macromolecules binds jh with an approximate affinity of k(d) of 10 nm. in leucophaea the concentration of lp is augmented by jh during vitellogenesis at the same time when vg is induced de novo. the circulating levels of each of lp and vg at mid-vitellogenesis are in the 10 microm range. similar values h ... | 2000 | 10876123 |
| purification and amino acid sequence of a highly insecticidal toxin from the venom of the brazilian spider phoneutria nigriventer which inhibits nmda-evoked currents in rat hippocampal neurones. | a new insecticidal toxin tx4(5-5) was isolated from the fraction phtx4 of the venom of the spider phoneutria nigriventer by reverse phase high performance liquid chromatography (hplc) and anion exchange hplc. the complete amino acid sequence determined by automated edman degradation showed that tx4(5-5) is a single chain polypeptide composed of 47 amino acid residues, including 10 cysteines, with a calculated molecular mass of 5175 da. tx4(5-5) shows 64% of sequence identity with tx4(6-1), anoth ... | 2001 | 10978749 |
| allatotropin-like neuropeptide in the cockroach abdominal nervous system: myotropic actions, sexually dimorphic distribution and colocalization with serotonin. | allatotropin (at) was isolated from the moth manduca sexta as a peptide stimulating biosynthesis of juvenile hormone in the corpora allata, but has also been shown to be cardioactive in the same species. here, we have investigated the presence and biological activity of at-like peptide in the cockroaches leucophaea maderae and periplaneta americana with focus on abdominal ganglia and their target tissues. an antiserum to m. sexta at was used for immunocytochemical mapping of neurons in the abdom ... | 2000 | 11058230 |
| expression of the american cockroach per a 1 allergen in mammalian cells. | cockroach allergens are one of the major etiologic risk factors for developing ige-mediated allergic respiratory illness throughout the world. per a 1 is a cross-reactive allergen of american and german cockroaches. this study aimed to investigate the expression of a recombinant american cockroach (periplaneta americana) per a 1, c42, allergen in mammalian cos-1 cells. | 2000 | 11117276 |
| sequence analysis of the first complete cdna clone encoding an american cockroach per a 1 allergen. | c3, designated as per a 1.0103 according to who/iuis nomenclature, encoding a novel american cockroach allergen of 395 aa (44.6 kda), is the first complete cdna clone with a translatable immunoreactive protein among the reported group 1 cockroach allergens. its deduced amino acid sequence possesses a signal sequence, phosphorylation sites, mitochondrial energy transfer protein signatures, and four repeats each sharing striking similarity with the corresponding repeats of the other cockroach alle ... | 2000 | 11118630 |
| methanomicrococcus blatticola gen. nov., sp. nov., a methanol- and methylamine-reducing methanogen from the hindgut of the cockroach periplaneta americana. | a small irregular coccoid methanogenic bacterium (pat) was isolated from the hindgut of the cockroach periplaneta americana. fluorescence microscopy and transmission electron microscopy of the hindgut of p. americana suggest that the organism occurs abundantly in the microbiota attached to the hindgut wall. the strain produces methane by the reduction of methanol and methylated amines with molecular hydrogen. acetate, coenzyme m, yeast extract, tryptic soy broth and vitamins are required for gro ... | 2000 | 11155972 |
| electrophysiological analysis of the neurotoxic action of a funnel-web spider toxin, delta-atracotoxin-hv1a, on insect voltage-gated na+ channels. | the effects of delta-actx-hv1a, purified from the venom of the funnel-web spider hadronyche versuta, were studied on the isolated giant axon and dorsal unpaired median (dum) neurones of the cockroach periplaneta americana under current- and voltage-clamp conditions using the double oil-gap technique for single axons and the patch-clamp technique for neurones. in parallel, the effects of the toxin were investigated on the excitability of rat dorsal root ganglion (drg) neurones. in both drg and du ... | 2001 | 11171353 |
| survival of aedes aegypti (diptera: culicidae) eggs in surface and subterranean breeding sites during the northern queensland dry season. | the effect of a protracted dry season on the viability of ae. aegypti (l.) eggs was examined in townsville, northern queensland, australia. eggs were placed in several different surface and subterranean larval habitats; and after four dry season months, only 1-10% of eggs remained viable in the surface and subterranean sites, respectively. low humidity and predation by periplaneta americana (l.) were the major causes of egg mortality in eggs in surface sites. p. americana was the most significan ... | 2001 | 11372971 |
| isolation and characterization of a chryseobacterium strain from the gut of the american cockroach, periplaneta americana. | a 16s rdna sequence cloned directly from whole-gut microbiota of the american cockroach, periplaneta americana, indicated the presence of a member of the bacteroides/flavobacterium group most closely related to the genus flavobacterium. in an attempt to confirm this finding, we isolated a yellow-pigmented bacterium (strain fr2) from the hindgut of this insect. strain fr2 was phylogentically and phenotypically most similar to species of flavobacterium and related bacteria, namely chryseobacterium ... | 2001 | 11382221 |
| reinvestigated life history of moniliformis dubius meyer, 1933 in chiang mai, thailand. | the lite cycle of moniliformis dubius meyer, 1933 was investigated by using rats (rattus norvegicus) as the definitive host and american cockroach (periplaneta americana) as an intermediate host. at room temperature (22-26 degrees c), the shelled acanthor hatched in mid-gut of p. americana 3 days postinfection. released to be the acanthor and then penetrated the gut wall into the hemocoel 5 days postinfection. it further developed into acanthella with proboscis primordium 16 days postinfection. ... | 2000 | 11414462 |
| cockroach midgut peptides that regulate cell proliferation, differentiation, and death in vitro. | the number of insect midgut cells is maintained homeostatically in vivo and in vitro. however, during starvation, the midgut shrinks and the rate of cell replacement appears to be suppressed. when they undergo metamorphosis, the internal organs of insects are drastically remodeled by cell proliferation, differentiation, and apoptotic processes, and the net number of cells usually increases. an extract of 1650 midguts of periplaneta americana was fractionated by high-performance liquid chromatogr ... | 2001 | 11515965 |
| insecticidal activity of essential oils: octopaminergic sites of action. | a study was conducted to determine the insecticidal activity and mechanism of action of three essential oils (eugenol, alpha-terpineol and cinnamic alcohol) and an equal part mixture (3-blend) against american cockroaches (periplaneta americana). to address species differences in response to treatment with the test oils, carpenter ants (camponotus pennsylvanicus de geer), and german cockroaches (blattella germanica) were included in this study. exposed american cockroaches demonstrated hyperacti ... | 2001 | 11701389 |
| arthromitus (bacillus cereus) symbionts in the cockroach blaberus giganteus: dietary influences on bacterial development and population density. | the filamentous spore-forming bacterium arthromitus, discovered in termites, millipedes, sow bugs and other soil-dwelling arthropods by leidy (1850), is the intestinal stage of bacillus cereus. we extend the range of arthromitus habitats to include the hindgut of blaberus giganteus, the large tropical american cockroach. the occurrence and morphology of the intestinal form of the bacillus were compared in individual cockroaches (n=24) placed on four different diet regimes: diurnally maintained i ... | 1999 | 11762374 |
| cockroach surveys in 14 provinces of thailand. | cockroach surveys using sticky traps were conducted in urban areas of 14 thailand provinces. at least 30 houses in each province were randomly sampled for cockroaches. each house was trapped in three areas: kitchen, bedroom and outside. a total of 2,648 cockroaches was caught by 550 out of 1,542 traps (35.7%), from 337 of the 514 houses (65.6%). overall, relative density ranged from 2.6 to 9.1 with an average of 5.2 cockroaches/house. on the average, 47.7% of the cockroaches were caught in the k ... | 2001 | 11813661 |
| prostaglandin biosynthesis by midgut tissue isolated from the tobacco hornworm, manduca sexta. | we describe prostaglandin (pg) biosynthesis by isolated midgut preparations from tobacco hornworms, manduca sexta. microsomal-enriched midgut preparations yielded four pgs, pga/b(2), pgd(2), pge(2) and pgf(2alpha), all of which were confirmed by analysis on gas chromatography--mass spectrometry (gc--ms). pga and pgb are double bond isomers which do not resolve on tlc but do resolve by gc; for convenience, we use the single term pga(2) for this product. pga(2) was the major product under most con ... | 2002 | 11886778 |
| the periviscerokinin (pvk) peptide family in insects: evidence for the inclusion of cap(2b) as a pvk family member. | periviscerokinins (pvks) are a distinct insect peptide family with unusual distribution in the central nervous system and neurohemal release sites. pvks were first isolated from the abdominal perisympathetic organs of periplaneta americana, but can be found in other insect species. peptides with structural similarity to pvks have been identified through searches of the drosophila genome. the cardioacceleratory peptide cap(2b) of the hawkmoth manduca sexta shares close amino acid identity with th ... | 2002 | 11897378 |
| [enterobacteria isolated from cockroaches (periplaneta americana) captured in a brazilian hospital]. | to isolate and identify microorganisms from cockroaches that were captured in a public hospital and to test the antimicrobial susceptibility of these microorganisms. | 2002 | 11939120 |
| characterization and comparison of commercially available german and american cockroach allergen extracts. | in this study we examine the variability among unstandardized cockroach allergen extracts. | 2002 | 11994096 |
| ige reactivity of tandem repeats derived from cockroach allergen, bla g 1. | sensitization to cockroach allergens is associated with the development of asthma. bla g 1 is a german cockroach allergen that shows allergenic cross-reactivity with american cockroach allergen, per a 1, and has a molecular structure composed of multiple tandem amino-acid repeats. two consecutive repeats are not identical but form a duplex that constitutes a basic molecular unit of bla g 1. by molecular mass, purified natural bla g 1 would contain approximately two duplexes. we investigated the ... | 2002 | 12071974 |
| a chimera polypeptide with active sites of hwtx-i and aai. | the functional amino acid sequence and its neighbouring fragments in the molecule of amaranth alpha-amylase inhibitor isolated from seeds of the mexican crop plant amaranthus hypochondriacus were transferred, by solid phase chemical synthesis, into n-terminal region of the huwentoxin-i(hwtx-i). the synthetic chimera polypeptide was confirmed by edman degradation and maldi-tof mass spectroscopy. the formation of three disulfide bonds and special conformation of the synthetic chimera was induced b ... | 2000 | 12075454 |