| comparison of a multi-allergen dipstick ige assay to skin-prick test and rast. | a multi-allergen dipstick enzyme immunoassay 'quidel allergy screen' (qas) has recently been developed commercially for measuring ige antibodies against nine allergens (house dust 1, house dust 2, dermatophagoides pteronyssinus, d. farinae, japanese cedar, ragweed, cat dander, sweet vernal grass, and egg white) at one time. to assess whether this assay is useful in screening allergen-specific ige antibody, we compared the titres of ige antibodies against the nine allergens measured by qas to tho ... | 1990 | 2192782 |
| the control of house dust mite allergens in rugs. | used rugs were vacuum cleaned, "wet cleaned," "shampooed," or heated in an autoclave. the effects on the live-mite population, on the allergen (dermatophagoides pteronyssinus, der p i and der p ii; and dermatophagoides farinae, der f ii) content, and on the suitability of the rugs as a habitat for house dust mites was studied. autoclaving was most effective in all three aspects. the cleaning methods were quite inefficient for killing the mites, but a significant reduction of allergens and deteri ... | 1990 | 2229844 |
| antigenic analysis of group i house dust mite allergens using random fragments of der p i expressed by recombinant dna libraries. | antigenic regions of a major house dust mite allergen, der p i, were identified by a recombinant dna strategy employing the technique of random fragmentation. fragments of cdna coding for der p i were produced by sonication and used to construct lambda gt 11 expression libraries. analyses of recombinant fragments reactive with a rabbit anti-der p i antiserum showed that the b cell determinants expressed in escherichia coli were limited, with the majority (86%) of antigenic clones isolated mappin ... | 1990 | 2246073 |
| [results of patch test using mite components in atopic dermatitis (ad) patients. 1st report. "as is patch test" using crushed mites]. | as is patch test using crushed live mites and crushed dried dead mites of dermatophagoides pteronyssinus (d.p.), dermatophagoides farinae (d.f.), & tryophagus ptrescentiae (t.p.) was performed on 49 ad patients and 18 control patients suffering from dermatitis other than ad. results: 1) with crushed dried dead mites all patients showed negative reactions but with live mites 11 among 49 ad patients were positive (22.4%); 2) female mites were more positive than males at the rate 8:1; 3) with cases ... | 1990 | 2273575 |
| house dust mite allergy in florida. mite survey in households of mite-sensitive individuals in tampa, florida. | this study evaluated the prevalence of positive house dust mite skin tests in a population of atopic individuals and identified the mite species present in mattress and house dust samples in homes of the tampa bay area. four hundred consecutive individuals were evaluated for respiratory complaints and skin tested with standardized extracts of dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df). two hundred forty individuals (60%) had a positive skin test to the mite extracts. d ... | 1990 | 2292372 |
| the prevalence of allergic disease and ige antibodies to house dust mite in schoolchildren in taiwan. | the prevalence of positive specific ige antibodies to house dust mites (dermatophagoides pteronyssinus; d. farinae) was determined by enzyme-linked immunosorbent assay (elisa) in 5097 (61%) volunteers of 8345 schoolchildren aged between 7 and 14 yr from two government schools. all of them filled out a questionnaire concerning allergic symptoms. among them, 412 (8.1%) children showed a positive reaction to at least one of the two mite allergens, the range varying between 5.6 and 11.2% according t ... | 1990 | 2310980 |
| [seasonal variations in house dust mite allergens--significance for children with allergy]. | allergenic proteins of the house dust mites dermatophagoides pteronyssinus and dermatophagoides farinae and their major allergens der p i and der f i are one of the most significant environmental factors which may facilitate sensitization and allergic airway disease in childhood. in order to measure seasonal variations of mite allergen exposure, we collected mattress dust samples monthly in 13 homes over a period of 1 year. der p i and der f i were determined with a sandwich type elisa. mite all ... | 1990 | 2320013 |
| dermatophagoides and storage mites: statistical analysis of rast results. | r.i.a. dosages of specific ige antibodies to dermatophagoides pteronyssinus, dermatophagoides farinae (house dust mites), acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae (storage mites) in 467 unselected patients were statistically analyzed. a significant noncorrelation between specific ige antibodies to storage mites and those to house dust mites has been found, suggesting that storage mites have their own allergenic specificity. | 1990 | 2321810 |
| localization of antigens and allergens in thin sections of the house dust mite, dermatophagoides pteronyssinus (acari: pyroglyphidae). | cryostat sections of the house dust mite, dermatophagoides pteronyssinus, were probed in fluorescent microscopy studies with rabbit polyclonal and mouse monoclonal antibodies specific for mite allergens including the major allergen, der p i. sections also were probed for allergens with sera from human mite-allergic subjects containing ige antibodies to mite allergens and with lectins. antibody binding was mainly to the gut lining and gut contents of the mite, although some specific labeling also ... | 1990 | 2332879 |
| isolation of cdna coding for the major mite allergen der p ii by ige plaque immunoassay. | a lambda gt11 library made with cdna from the house dust mite dermatophagoides pteronyssinus was screened with human allergic serum by ige plaque radioimmunoassay. this resulted in the isolation of clones coding for the major allergen der p ii. the cdna coded for a 129-residue protein of 14,131 daltons with no n-glycosylation sites. no sequence homology with other proteins was evident. the der p ii expressed in escherichia coli reacted with ige in 14 of 17 sera from mite-allergic patients giving ... | 1990 | 2341191 |
| immune response to blomia kulagini and dermatophagoides pteronyssinus in sweden and brazil. | the immune responses to the non-pyroglyphid mite blomia kulagini and the pyroglyphid mite dermatophagoides pteronyssinus were compared in 440 swedish farmers and 34 allergic subjects from brazil using the radioallergosorbent test (rast). both b. kulagini and d. pteronyssinus were shown to be a common cause of sensitization, particularly in brazil. the highest rast scores were found against d. pteronyssinus in sera from both countries. allergenic cross-reactivity between the two mites was studied ... | 1990 | 2341199 |
| a study of the relationship between childhood nephrotic syndrome and allergic diseases. | the incidence of atopic diseases in 206 children with nephrotic syndrome (ns) was studied. boys with ns had three times higher incidence of bronchial asthma than the general population. there was no difference in the girls. both boys and girls with ns had about three times more allergic rhinitis and ten times more atopic dermatitis than the general population. in ns patients with associated allergic disease, skin test and allergen-specific ige antibodies by radioallergosorbent test (rast) were p ... | 1990 | 2347227 |
| changes of serum-specific ige antibody titer during hyposensitization in mite-sensitive asthmatic children. | in this study, we used the enzyme-linked immunosorbent assay (elisa) to evaluate the changes of ige antibody titer against dermatophagoides pteronyssinus (d. pteronyssinus) or dermatophagoides farinae (d. farinae) in asthmatic children after immunotherapy. according to elisa analysis, a significantly higher mean level of ige antibody titer to d. pteronyssinus (or d. farinae) was found in nonhyposensitized asthmatic children than in the pediatric control group (p less than 0.001), but there was n ... | 1990 | 2347816 |
| assessment and control of house dust mite infestation. | most allergens of the house dust mite (dermatophagoides pteronyssinus and dermatophagoides farinae) are carried by the mite excreta rather than by the mites themselves. a method of assessing the concentration of mite excreta in dust (the acarex test) is described. the effects of acarosan products, whose active ingredient is solidified benzyl benzoate, on mites and mite excreta were evaluated. over a period of three years, three to four applications of acarosan effectively destroyed mites in carp ... | 1990 | 2379225 |
| functional characterization of lymphocyte response to fractionated house dust mite antigens (dermatophagoides pteronyssinus) in atopic and non-atopic individuals. | this study investigated the cellular response of human lymphocytes to dermatophagoides pteronyssinus crude antigen and 14 molecular weight (mw) fractions. the cells were derived from atopic patients and healthy individuals who were skin test-positive or skin test-negative to mite antigen. when stimulated with crude antigen, the group of patients showed elevated proliferation and production of lymphokine in comparison with the healthy skin test-negative individuals (p less than 0.01). by stimulat ... | 1990 | 2379943 |
| [the histamine liberation test as a diagnostic parameter and its possible modification following specific immunotherapy]. | it is generally accepted that the allergic reaction depends on the levels of specific ige, on the concentration of blocking antibodies and on the capacity of the basophils to release mediators. the fact that in the histamine release test (hrt) all of the elements intervening in an allergic reaction (antigens, ige, basophils and mononuclear cells of a lymphoid origin) have been found, has led some authors to consider it as the most suitable method of "in vitro" diagnosis. this is a reflection of ... | 1985 | 2408457 |
| purification and partial characterization of two major allergens from the house dust mite dermatophagoides pteronyssinus. | two major allergens of the house dust mite, dermatophagoides pteronyssinus (dp), were purified, and their molecular weight and isoelectric points (pis) were determined. dp 42 was purified from an acetone-precipitated mite-excrement extract by a combination of hydrophobic interaction chromatography on phenyl sepharose and copper-chelate chromatography. the molecular weight was determined to be 18,000 and 25,000 to 30,000 by gel filtration (g-75) and sodium dodecyl sulphate-polyacrylamide gel elec ... | 1985 | 2414353 |
| studies on bronchial hyperreactivity, allergic responsiveness, and asthma in rural and urban children of the highlands of papua new guinea. | the prevalence of asthma and allergic responsiveness in rural and urban children of the highlands of papua new guinea was studied. bronchial provocation studies with histamine demonstrated significant bronchial hyperreactivity in 0.5% (1 in 195) rural and 1.7% (1 in 59) urban children, rates which were significantly lower than those observed in corresponding adult populations (7%). urban children demonstrated a higher incidence of skin test reactivity toward dermatophagoides pteronyssinus, asper ... | 1986 | 2420852 |
| cross-reactivity between the house dust mite dermatophagoides pteronyssinus and the mange mites psoroptes cuniculi and p. ovis. i. demonstration of antibodies to the house dust mite allergen dpt 12 in sera from p. cuniculi-infested rabbits. | the cross-reactivity of the mange mites psoroptes cuniculi (pc) and psoroptes ovis (po) antigens with the house dust mite dermatophagoides pteronyssinus (dp) antigens has been studied. cross-reactivity between mange mite and house dust mite antigens was demonstrated by both elisa and immunoelectrophoresis by use of a sheep anti-dp antiserum. both pc and po were demonstrated to contain eight cross-reacting antigens. sera from rabbits infested with pc were demonstrated to produce antibodies to the ... | 1986 | 2426321 |
| antigen der f i from the dust mite dermatophagoides farinae: structural comparison with der p i from dermatophagoides pteronyssinus and epitope specificity of murine igg and human ige antibodies. | the physicochemical and antigenic properties of an allergen purified from dermatophagoides farinae, der f i, were compared with der p i from dermatophagoides pteronyssinus. on sds-page, der f i migrated as a single polypeptide chain with the same m.w. as der p i (24,000). two isoallergenic peaks of der f i were identified on preparative isoelectric focusing (pi 5.7 to 6.3 and pi 6.6 to 6.95). fractions from each peak were shown to have an identical amino acid composition (which was similar but n ... | 1986 | 2428875 |
| comparison by electroblotting of ige-binding components in extracts of house dust mite bodies and spent mite culture. | eight dermatophagoides pteronyssinus extracts (three culture extracts, four mite body extracts, and the world health organization international standard [is]) were investigated by side-by-side sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by electrotransfer to nitrocellulose, and by probing with a pooled serum from mite-allergic subjects. representative body and mite culture extracts were compared by probing with individual sera, and both types of extract were also compared by rast- ... | 1987 | 2433323 |
| investigations of culture medium-free house dust mites. iv. cross antigenicity and allergenicity between the house dust mites, dermatophagoides farinae and d. pteronyssinus. | heterologous crossed immunoelectrophoresis (cie) analysis demonstrated that dermatophagoides pteronyssinus body and d. farinae body extracts contained up to 21 cross-reacting antigens (ags). incubation of cie gels with two mite-sensitive individual sera and a serum pool of mite-sensitive patients indicated that up to nine cross-reacting ags bound mite-specific ige on crossed radioimmunoelectrophoresis and were common allergens. likewise, heterologous cie analysis of d. pteronyssinus feces and d. ... | 1987 | 2434550 |
| selection and characterization of monoclonal antibodies against a major allergen in dermatophagoides pteronyssinus. species-specific and common epitopes in three dermatophagoides species. | hybridomas were produced after immunization with der p i (ag 42, p1). specific anti-der p i activity was detected in a reverse elisa using immobilized anti-mouse ig and highly purified der p i labelled with horseradish peroxidase. stable subclones from 6 parental hybridomas were obtained. the species specificities of the monoclonal antibodies (mabs) were classified: by an overlayer technique analogous to the crie technique, and by two competitive elisa techniques. one mab recognized an epitope s ... | 1987 | 2440816 |
| epitope mapping of two major inhalant allergens, der p i and der f i, from mites of the genus dermatophagoides. | the repertoire of antigenic sites on two major dust mite allergens, der p i of dermatophagoides pteronyssinus and der f i of d. farinae, was studied using murine (balb/c) monoclonal antibodies (mab), polyclonal rabbit igg antibodies, and human ige antibodies. fifty-three igg mab were analyzed from six different fusions (five vs der p i, one vs der f i). by antigen binding radioimmunoassay (ria), most mab were either der p i or der f i specific, and only 2/53 bound to both allergens. epitope mapp ... | 1987 | 2442247 |
| cloned human t lymphocytes reactive with dermatophagoides farinae (house dust mite): a comparison of t- and b-cell antigen recognition. | in this report, t-cell and b-cell recognition of the house dust mite dermatophagoides farinae (d. far.) is compared. nitrocellulose immunoblots of polyacrylamide gel electrophoresis (sds-page)-fractionated d. far. were added to proliferation assays to map the antigen specificity of cloned human helper t cells and a long-term line induced with d. far. t-cell recognition was of a polypeptide of molecular weight 9000-13,000, that migrates with the serologically defined allergen der fii (12,500 mw). ... | 1987 | 2448229 |
| modification of house dust mite allergens by monomethoxypolyethylene glycol. allergenicity measured by in vitro and in vivo methods. | in animal models, allergen modification by coupling to monomethoxypolyethylene glycol (mpeg) molecules can reduce allergenicity of the extract and makes the allergen capable of suppressing boosted ige response. to investigate in a human system the degree of attenuation implied by a mpeg modification of a house dust mite (dermatophagoides pteronyssinus) extract, 55 adults with asthma caused by house dust mites were tested by skin prick test (spt) and histamine release assay (hr). rast inhibition ... | 1988 | 2448247 |
| passive sensitization of basophil leukocytes from non-atopic adult by plasma from allergic children. | basophil leukocytes from a non-atopic donor, who responded well to anti-ige, were depleted of their native membrane-bound ige by acid treatment and passively sensitized with plasma containing either phleum pratense-, dermatophagoides pteronyssinus- or dog dander- specific ige obtained from 18 allergic children. histamine release was then performed on the passively sensitized cells and the results were compared with those of bronchial provocation test (bpt), allergen-specific serum ige (rast), sk ... | 1988 | 2449829 |
| skin tests and histamine release with p1-depleted dermatophagoides pteronyssinus body extracts and purified p1. | monoclonal antibodies were raised against p1 and dp x, two major allergens in dermatophagoides pteronyssinus extracts. the concentrations of ige antibodies to p1 and dp x in sera of mite-sensitive patients were determined in rast with immunosorbent-bound monoclonal antibodies used for insolubilization of the allergens. the major allergen-specific ige titers were compared with the total ige response against d. pteronyssinus. the results of these serologic assays confirmed studies in the literatur ... | 1988 | 2453544 |
| latex allergosorbent test (last): a new immunoassay for specific ige with latex particles. | we describe a two-step latex (lx) agglutination assay for the titration of specific anti-dermatophagoides pteronyssinus ige. the samples are first incubated with allergen-coated lx of 2.3 microns diameter. bound ige is digested by pepsin and then titrated by its agglutinating activity on 0.8 micron lx particles coated with antihuman fc epsilon rabbit f(ab')2. this latex allergosorbent test detects 100 pg of specific ige per milliliter and does not depend on the concentration of total ige. owing ... | 1988 | 2455743 |
| release of highly-sulphated glycosaminoglycans and histamine from human basophils. | a new assay for highly-sulphated glycosaminoglycans (gags) has been applied to the study of allergen (dermatophagoides pteronyssinus)-induced release of gag from human basophils. highly-sulphated gag, which was not heparin, was found only in basophil-containing cellular fractions of whole blood. its release, like that of histamine, was ca++-dependent. however, allergen-induced release of gag from the basophils of atopic individuals exhibited a different time-course and dose-response from that of ... | 1988 | 2456991 |
| role of igg4 in histamine release from human basophil leucocytes. i. sensitization of cells from normal donors. | several conflicting reports on the ability of igg4 to mediate type i allergic reactions have appeared lately. we have developed a model system for testing this possibility, using passive sensitization of basophil leucocytes from normal individuals. at first, the system was optimized with regard to donor cells and anti-igg4 antibody: among 3 normal individuals with cells showing high, medium and low responses to anti-ige, only the high responder showed a reproducible, but low response to anti-igg ... | 1988 | 2457556 |
| mhc class ii restriction specificity of cloned human t lymphocytes reactive with dermatophagoides farinae (house dust mite). | in this report the antigen and restriction specificity of human t-cell clones induced with dermatophagoides farinae (d. farinae) and isolated from an atopic individual with perennial rhinitis has been investigated. of the six clones analysed, four were species specific and two showed cross-reactivity for the closely related dermatophagoides pteronyssinus (d. pteronyssinus). inhibition of antigen-dependent proliferation by murine monoclonal antibodies directed against hla-d-region gene products r ... | 1988 | 2459050 |
| loratadine, an antihistamine, blocks antigen-and ionophore-induced leukotriene release from human lung in vitro. | some h1-antihistamines possess anti-allergic properties, and inhibit the immunological release of mediators including histamine and sulfiopeptide-leukotrienes (slow reacting substance of anaphylaxis) from lung. the effects of the antihistiamine loratadine, sch29851, on the release of leukotrienes and histamine from human lung fragments were measured, using the calcium ionophore a23187 and an extract of antigen from dermatophagoides pteronyssinus, house dust mite, (with passively sensitized lung) ... | 1988 | 2470117 |
| strong delayed-type hypersensitivity induced against house dust mite antigens in the mice. | the aim of this study was to assess the antigenicity of house dust mite antigens using delayed-type hypersensitivity (dth) responses in mice. the crude extracts of dermatophagoides pteronyssinus and dermatophagoides farinae induced strong dth responses and was transferred with t cells. assessment of dth responses to the house dust revealed that substantial cross-reactivity at t-cell level was observed between these two mite extracts. almost all the fractions of the extract of d. pteronyssinus, w ... | 1989 | 2470689 |
| comparative analysis of physicochemical and immunochemical properties of the two major allergens from dermatophagoides pteronyssinus and the corresponding allergens from dermatophagoides farinae. | two major allergens, dp1 (der p i) and dp2 (der p ii), were isolated from the whole culture extract of dermatophagoides pteronyssinus, and the physicochemical and immunochemical properties of these allergens were compared with those of the corresponding allergens from dermatophagoides farinae, df1 (der fi) and df2 (der fii). on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, polyacrylamide gel isoelectric focusing and amino acid analysis, both dp1 and dp2 were demonstrated to have clo ... | 1989 | 2498214 |
| house dust mites in naval ships, military barracks, and homes in the hampton roads area of virginia. | this survey was undertaken to determine the possible exposure of military personnel to house dust mites in ships, barracks, and homes in the hampton roads area of virginia. during the period from april 1, 1982 through august 31, 1983, 524 dust samples were collected with vacuum cleaners. mites were extracted from aliquots of dust, counted, and identified as to species and life stage. dermatophagoides pterynssinus (european house dust mite) was more common in private homes, while dermatophagoides ... | 1989 | 2507967 |
| pyroglyphid mites in the high tatras sanatoria. | 1. in our work we studied the problematics of house dust fauna in the sanatoria and institutes of the tatra region, regarding especially the incidence of mites of the family pyroglyphidae that are considered for the major source of house dust allergen. 2. in eleven places of different altitude in this region, there were in 1982 chosen 13 localities--sanatoria and institutes. here, once quarterly, were collected house dust samples from beds and floors. 3. in the locality 1 helios, strbské pleso, ... | 1989 | 2519180 |
| [pathology caused by acari]. | this report concerns the observations the authors have been carrying out for over twelve years in sardinia; they regard the sensitization to house dust mites (dermatophagoides pteronyssinus, dermatophagoides farinae) and to storage mites (acarus siro, glycyphagus domesticus, lepidoglyphus destructor, tyrophagus putrescentiae) and are been obtained with: 1) skin tests with mite extracts; 2) radioallergosorbenttest (rast) for specific ige and igg; 3) mite exposure in homes and mite analysis; 4) hy ... | 1989 | 2519754 |
| evidence of paf-acether metabolic pathway activation in antigen challenge of upper respiratory airways. | lyso-paf-acether and paf-acether (formerly platelet-activating factor) were detected in nasal secretions from patients with hay fever who underwent local antigen challenge. lyso-paf release was observed in 12 of 13 patients, with a maximum (p less than 0.001) 5 min after stimulation and a progressive decrease during the first hour. paf was detected in the 5-min postchallenge nasal washings from two of 13 subjects. after hplc, this mediator was found in four of seven postchallenge nasal washings ... | 1989 | 2546468 |
| monoclonal anti-human igg antibodies for quantitation of allergen-specific igg in human sera. | eight monoclonal anti-human igg antibodies were fully characterized and evaluated as possible reagents in solid phase radioimmunoassay for quantitating allergen-specific igg antibody. four monoclonal antibodies (hg24d, hg2-14, hg2-18, and hg2-25) recognize ch2 domain of human igg and bind to human igg fixed to microtiter plate with high affinities. these monoclonal antibodies were more suitable than polyclonal rabbit anti-human igg antibody in phadebas rast for honey bee venom-specific igg antib ... | 1985 | 2578496 |
| leukocyte histamine release to suxamethonium in patients with adverse reactions to muscle relaxants. | in an earlier study we confirmed the usefulness of intradermal skin tests and histamine release in diagnosis of patients reactive to muscle relaxants, and we suggested an ige-mediated reaction rather than an idiosyncratic mechanism. in a later study, we studied the relationship between (formula: see text) that is one of the muscle relaxants producing the most frequent adverse reactions under anesthesia. histamine release was measured in five patients with increasing concentrations of suxamethoni ... | 1985 | 2579116 |
| measurement of allergens associated with dust mite allergy. i. development of sensitive radioimmunoassays for the two groups of dermatophagoides mite allergens, der i and der ii. | sensitive radioimmunoassays were developed to measure the two groups of major allergens (der i and der ii) of dermatophagoides pteronyssinus and dermatophagoides farinae, in which radiolabeled protein a served as a general tracer. glass rods covalently coupled with f(ab')2 fragments of rabbit antiserum igg were incubated first with mite or house dust extracts, and then with affinity-purified rabbit antibodies. the bound allergen-antibody complex was detected with a 125i-labeled protein a. the tw ... | 1989 | 2583856 |
| [skin reactivity to grass pollen and the mite dermatophagoides pteronyssinus in the prick-test using 2 types of allergen preparations]. | the reliability of prick testing with allergen preparations produced in yugoslavia was assessed by comparison to the internationally accepted ones (pharmacia diagnostica) of 100,000 bu activity. the following allergens were used: the mite dermatophagoides pteronyssinus in 107 subjects, and grass pollens: dactylis glomerata, poa pratensis, phleum pratense and secale cereale in 39 subjects. the clinical significance of the response was assessed in relation to the skin reaction to the negative cont ... | 1989 | 2638564 |
| [relationship between bronchial asthma and mite]. | we studied the relationship between bronchial asthma and mite as an antigen by reviewing previous reports. the incidence of the bronchial asthma has been increasing and it is estimated to be more than 2% of the total population. the main components of the antigen in the house dust (h, d.), which has been considered to be an important antigen of the perennial asthma, are dermatophagoides farinae (d, f.) and dermatophagoides pteronyssinus (d, p.) in h, d. the housing environment in recent years ha ... | 1989 | 2689700 |
| correlations between levels of mite and cat allergens in settled and airborne dust. | thirty homes in rochester, minn., 20 of which housed cats, were studied to compare cat and mite allergen concentrations in airborne and settled dust. with air sentinels in the bedroom and living room for airborne collections, and a sample vac for collections from living room carpet and bedroom mattress, immunochemical quantifications of each were made with various radiometric assays with polyclonal and monoclonal antibodies. the most significant correlations were found between airborne mite in t ... | 1989 | 2708737 |
| skin and serum reactivity to some storage mites in children sensitive to dermatophagoides pteronyssinus. | the incidence of skin test and rast positivity for dermatophagoides farinae and storage mites (glycyphagus privatus, lepidoglyphus destructor, tyrophagus putrescentiae, acarus siro, and gohieria fusca) have been evaluated in children with bronchial asthma and positive prick test to dermatophagoides pteronyssinus. the storage mite with highest incidence of skin and rast positivity was gohieria fusca, which caused a positive response in more than 50% of the children evaluated. this antigen appears ... | 1989 | 2742217 |
| immunogenicity of monomeric and polymeric house dust mite dermatophagoides pteronyssinus. | a methodology for the preparation of a polymerized antigen from house dust mites dermatophagoides pteronyssinus is described. polymerization was carried out by glutaraldehyde treatment in pbs and sephacryl s300 column fractionation. a polymer of 135 kd was obtained. rabbits immunized with the soluble polymer (hdmp) elaborated specific antibodies as measured by a dot-immunobinding assay. the reaction was inhibited by previous incubation with the hdmp antigen. it is suggested that the polymerized ... | 1989 | 2750636 |
| hypersensitivity to pets in italy. | eight hundred and two consecutive symptomatic outpatients (with asthma and rhinitis) were studied to evaluate the incidence of prick test positivity to cat and dog danders and the association of exposure to these animals in the home, as revealed by a standard questionnaire. 14.3% were found to be skin positive to cat and/or dog danders. the incidence of cutaneous hypersensitivity to cat and/or dog danders was 25% in the 216 subjects who kept animals, significantly higher (p less than 0.0005) tha ... | 1989 | 2787593 |
| the pharmacology of leukotrienes in human airways: in vitro and in vivo studies. | immunological challenge of human lung parenchyma causes formation of arachidonate metabolites: prostaglandin d2 (pgd2) (70% of the formed mediators), leukotrienes e4 (lte4) (15%) and d4 (ltd4) (10%). leukotriene b4 (ltb4) was barely detectable (2%). inhibition of pgd2 formation by indomethacin (15 microm) was approximately 90%, but was not accompanied by redistribution of arachidonate metabolism towards sulphidopeptide leukotrienes, as postulated for aspirin-sensitive asthma. specific binding si ... | 1989 | 2803405 |
| the study of house dust mites in taiwan. | by using a radioallergosorbent test (rast) and intradermal skin testing, our previous studies strongly suggested that house dust mites are the most important allergen in taiwan, especially in asthmatic children. this study was conducted to determine the distribution of house dust mites in houses of mite-sensitive asthmatic children and normals, the species of mites in various house dust samples, the seasonal prevalence, and the relation between the content of mite allergen and the mite density i ... | 1989 | 2919796 |
| clinical significance and allergenic cross-reactivity of euroglyphus maynei and other nonpyroglyphid and pyroglyphid mites. | the clinical significance and allergenic cross-reactivity of the storage mites lepidoglyphus destructor and acarus siro and the house dust mites dermatophagoides pteronyssinus and euroglyphus maynei were investigated with specific ige antibodies by use of the rast and the rast-inhibition technique. rast-positive sera were obtained from 64 swedish farmers whose test results were positive to at least one of the four mite species. e. maynei was shown to be a common cause of sensitization among the ... | 1989 | 2926080 |
| [biochemical structure and allergenic activity of dermatophagoid mites (dermatophagoides pteronyssinus)]. | | 1988 | 2971865 |
| correlation between serum ige and specific ige antibody titer to house dust mite in children with asthma. | using the enzyme-linked immunosorbent assay (elisa) for ige specific to mites, we evaluated the relationship between total serum ige levels and ige antibodies specific to mite in 58 asthmatic children. our results showed that there was a positive correlation between total serum ige and ige antibody specific to dermatophagoides pteronyssinus (r = 0.57; p less than 0.001) and dermatophagoides farinae (r = 0.59; p less than 0.001). there was also a significant correlation between d. pteronyssinus-s ... | 1988 | 3182578 |
| comparison between modified skin prick test with standardized allergen extracts and phazet. | skin prick tests (spts) represent one of the most common techniques of skin testing and are of great value, especially when standardized extracts are used. phazet is a puncture test involving needles coated with standardized allergens. phazet and spts have been compared in 130 subjects tested with six standardized allergen extracts: orchard-grass, olive, and mugwort pollens, cat dander, dermatophagoides pteronyssinus, and alternaria. patients have been carefully selected on the basis of a sugges ... | 1988 | 3192871 |
| hypersensitivity to wheat flour in bakers. | eleven bakers and 4 pastry cooks with hypersensitivity to wheat flour underwent skin tests to flours (wheat, barley, rye, oats), pollens (wheat, barley, rye, oats, grasses), mites (dermatophagoides pteronyssinus, dermatophagoides farinae), molds (alternaria, aspergillus) and weeds (parietaria judaica). the levels of serum immunoglobulins (igg, iga, igm and ige) were studied and the values of specific ige (rast) in the presence of wheat flour and the histamine release test for wheat flour, grass ... | 1988 | 3228051 |
| sensitivity to parietaria pollen in the southampton area as determined by skin-prick and rast tests. | the pollen of parietaria species is a well-recognized and important inhalant allergen in the mediterranean area. parietaria judaica (pellitory-of-the-wall) is native to the u.k., flowering from june to september, but is not usually considered to be of any clinical importance by u.k. allergists. we skin tested 62 patients with a clinical history of summer seasonal respiratory symptoms and a control group of 11 patients with perennial respiratory symptoms only. each was skin tested in duplicate wi ... | 1988 | 3242974 |
| human immune response to allergens of house dust mite, dermatophagoides pteronyssinus. iv. occurrence of natural autologous anti-idiotypic antibodies. | igg isolated from the plasma of seven individuals hypersensitive to the common house dust mite dermatophagoides pteronyssinus (dpt) was exhaustively adsorbed onto insolubilized dpt. the unbound fraction was found by radioimmunoassay to contain antibodies recognizing the variable region of both anti-dpt igg and ige antibodies. this recognition was idiotype (id)-specific as it persisted after passage over insolubilized polyclonal igg of unrelated specificity. most of these anti-id igg carried the ... | 1988 | 3257924 |
| optimal efficacy of a fungicide preparation, natamycin, in the control of the house-dust mite, dermatophagoides pteronyssinus. | the efficacy of a fungicidal preparation, natamycin, for the effective control of dermatophagoides pteronyssinus (trouessart), was determined at different concentrations on mattress-dust microsites, including ticking. one treatment with the commercially available natamycin concentration (tymasil) resulted in a 65% reduction of house-dust mite populations within 4 weeks of treatment. the acaricidal control could be proven despite the protective role of mattress fibres such as ticking. however, mi ... | 1988 | 3259916 |
| human immune response to allergens of house dust mite, dermatophagoides pteronyssinus. v. auto-anti-idiotypic antibody characterization and cross-reactivity. | from the serum of 10 allergic subjects we have prepared igg antibodies recognizing idiotopes carried by specific antibodies to dermatophagoides pteronyssinus (dpt) allergens, and studied cross-reactivity of anti-dpt igg bystander and antigen-binding site-associated idiotopes by latex agglutination assays. idiotopes of specific anti-dpt ige were evaluated by radioimmunoassays. depending on the assay, a binding or inhibition of more than 50%, as compared to the reactivity of specific antibodies wi ... | 1988 | 3261244 |
| house dust mites and vernal keratoconjunctivitis. | the possible causative relationship between exposure to house dust mite allergen (hdma) and symptoms of vernal keratoconjunctivitis (vkc) was investigated. vkc patients were evaluated for an average of 18 months using patient reports of symptoms, physicians' observations and assays of sera and tears for total and specific ige to 10 inhalant allergens common in israel, including hdma. tear and serum samples from a group of 8 patients reacted only with hdma, and were compared with the tear and ser ... | 1988 | 3262844 |
| improved diagnosis and treatment of allergic rhinitis by the use of nasal provocation tests. | a simple and safe method of nasal provocation for out-patients that requires no special equipment is described. a total of 2,645 cases were tested by nasal provocation tests. of the total, 1,175 were tested with dermatophagoides pteronyssinus, 825 with mixed grass pollens, 352 with plantain (plantago lanceolata), and 125 for cats and correlating nasal tests with skin tests yielded 80%, 90%, and 85%, respectively. immunotherapy for allergic rhinitis was only considered justifiable when the nasal ... | 1988 | 3337397 |
| probit analysis applied to the allergen dose-response curve: a method for epidemiologic surveys. | the degree of skin sensitivity to five common allergens (grass, dermatophagoides pteronyssinus, mugwort, birch, and parietaria) was determined by the threshold dilution technique in all the skin test reactors of a random sample of 295 schoolchildren (142 male and 153 female subjects, age range 11 to 14 years), and the frequency distribution of responders at each concentration was analyzed by probit analysis. the potency of each allergen was presented in terms of median effective dose (ed50), and ... | 1988 | 3339189 |
| antigenic analysis of household dust samples. | household dust samples from the homes of 106 allergy clinic patients in baltimore were analyzed for specific allergen content. dust mite antigen content was determined by enzyme-linked immunosorbent assays (elisa) specific for the major allergens of dermatophagoides pteronyssinus, d. farinae, and d. microceras. cat and dog antigen content were determined by elisa using antisera for fel d 1 (formerly cat allergen 1) and dog allergens 3 and 13, respectively. mold content was assessed by culture wi ... | 1988 | 3341627 |
| human immune response to allergens of house dust mite, dermatophagoides pteronyssinus. iii. cross-reactivity of bystander idiotopes on allergen-specific ige antibodies. | rabbits were inoculated with affinity-purified human antibodies to the common house dust mite dermatophagoides pteronyssinus (dpt) to produce anti-idiotypic (id) antibodies. the id carried by human ige antibodies were studied in a radioimmunoassay in which specific anti-dpt ige were first insolubilized on rabbit f(ab')2 fragments against human fc epsilon. in a single individual specific anti-dpt ige antibodies shared bystander idiotopes (i.e. idiotopes located outside the antigen-binding site) w ... | 1988 | 3345795 |
| mite antigen and allergen contents of house dust samples. | the house dust mite (dermatophagoides pteronyssinus) antigen and allergen contents were measured by enzyme-linked immunosorbent assay (elisa) with enzyme-labelled anti-human ige and anti-mite rabbit igg antibodies. antigen content was high in dust samples from homes of patients with allergy but not in samples from homes of patients with kawasaki disease or of normal control subjects. allergen content was high in dust samples from homes of kawasaki disease patients. however, the values overlapped ... | 1988 | 3364213 |
| the binding of mouse hybridoma and human ige antibodies to the major fecal allergen, der p i, of dermatophagoides pteronyssinus. relative binding site location and species specificity studied by solid-phase inhibition assays with radiolabeled antigen. | the relative binding site location and species specificity of 19 mouse hybridoma antibodies, produced in four laboratories, to dermatophagoides pteronyssinus major fecal allergen, der p i, was studied by using immobilized mab and inhibitions of radiolabeled ag binding. four mab groups were defined, within which 4, 6, 8, and 5 mab, respectively, cross-inhibited each other. five mab were members of both group 2 and 3, demonstrating a considerable overlap of epitopes between the corresponding antib ... | 1988 | 3372999 |
| the pattern of human late-phase skin reactions to extracts of aeroallergens. | the kinetics and dose response of cutaneous late phase reactions (lpr) (defined as greater than 5 mm induration 6 hours after intradermal challenge with allergen) were studied in 20 randomly selected subjects with atopic rhinitis. when subjects were challenged intradermally with 0.2 biologic unit (bu) of dermatophagoides pteronyssinus or grass pollen, wheal-and-flare reactions were elicited without subsequent lprs. with 30 bu, all subjects developed lprs, whereas 1 and 10 bu provoked lprs in 28% ... | 1988 | 3379223 |
| human immune response to allergens of house dust mite, dermatophagoides pteronyssinus. isotypic analysis of antibodies in atopic and non-atopic subjects. | specific antibodies isolated by immunoabsorption on four main insolubilized allergens from dermatophagoides pteronyssinus (dpt) had the following isotypic distribution: in 16 atopic patients, 52% igg, 40% igm, 8% iga, 0.1% ige and, in 12 non-atopic individuals, 48% igg, 46% igm, 6% iga, 0.03% ige. the ratios between geometric means of antibody values in each class (atopic vs non-atopics) were 2.4 for igg, 2.0 for igm, 2.8 for iga and 66.7 for ige. the amount of anti-dpt antibodies in igg subclas ... | 1988 | 3414913 |
| [role of rhinomanometry in childhood mite allergy apropos of 76 cases]. | place for passive anterior rhinomanometry (par) in mite allergic children. par is a simple method to investigate chronic or recurrent rhinopathies in children. among 59 investigations with a dermatophagoides pteronyssinus extract, we found 27 positive responses, 3 doubtful results and 32 negative explorations. nasal sensitization is usually important: the liminal dose is less than 250 mcg in 17/28 cases. most children have no bronchial sensitivity. none of 14 children with negative cutaneous and ... | 1986 | 3453726 |
| [comparative study of various technics for measuring serum ige levels]. | in this work we report the results of a comparative study of different techniques to measure specific ige in sera. three methods were used, phadebas rast, phadezym rast (pharmacia) and allergen-specific fast+ test (3m diagnostics): 127 sera were tested with several pneumoallergens (housedust, dermatophagoides pteronyssinus, dactylis glomerata pollen, cat and dog hair). analysis of the results shows the following correlations: phadebas and phadezym rast 92%; phadebas rast and fast+ 88%; phadezym ... | 1987 | 3453730 |
| differences in development time, mortality and water loss between eggs from laboratory and wild populations of dermatophagoides pteronyssinus (trouessart, 1897) (acari: pyroglyphidae). | a total of four microcultures of adults of dermatophagoides pteronyssinus, two each from laboratory and wild populations, were fed on separate diets of house dust and yeast granules. a total of 35 eggs of known age from each of the four microcultures were harvested and incubated at 15 degrees c, 60% rh for 16 h/day and 30 degrees c, 75% rh for 8 h/day to simulate diurnal microclimatic fluctuations in a bed. eggs from females fed on yeast were larger and underwent more rapid rates of water loss, ... | 1987 | 3453786 |
| release of prostaglandin d2 into human airways during acute antigen challenge. | among the many possible mediators of the early asthmatic response, prostaglandin d2, a bronchoconstrictor, is the principal cyclooxygenase metabolite of arachidonic acid that is released upon the activation of mast cells and is also synthesized by human alveolar macrophages. we performed bronchoalveolar lavage in five patients with chronic stable asthma, before and up to nine minutes after local provocative challenge with dermatophagoides pteronyssinus. the lavage fluid was analyzed for products ... | 1986 | 3462506 |
| [risk indicators in reagin allergy. iii. ige specificities and family history. description of a genetic index for sorting patients for studying constitutional markers]. | the results of rasts were correlated with family history (interview referenced) of respiratory diseases in 1,477 children under the age of 13, referred for a presumed atopic respiratory disease. specific ige found to dermatophagoides pteronyssinus, cat's epithelium and food (egg white and cow milk) were not related to family history. significant increase of frequency of specific ige to timothy grass pollen was demonstrated. the author then describes a "genetic score" in groups of patients classi ... | 1986 | 3468822 |
| predominance of mite allergy over allergy to pollens and animal danders in a farming population. | the prevalence of ige-mediated hypersensitivity to respiratory allergens, including lepidoglyphus destructor (a storage mite) and dermatophagoides pteronyssinus, was studied in a rural population of gotland, a swedish island in the baltic sea. a sample of 440 farmers underwent examination, comprising skin-prick tests (animal danders, pollens, moulds, house dust mite and wheat) and blood sampling for radio-allergosorbent tests (rast) (birch, timothy grass, dog, d. pteronyssinus, cladosporium and ... | 1987 | 3499998 |
| eczematous reactions in atopic patients caused by epicutaneous testing with inhalant allergens. | to determine whether inhalant allergens could induce eczematous lesions we studied 17 patients with atopic eczema (with or without allergic rhinitis), 13 patients with allergic rhinitis without atopic eczema and 10 healthy control subjects. the allergens, birch pollen (betula verrucosa) and house dust mite (dermatophagoides pteronyssinus), were applied in aluminium chambers for 48 h on clinically normal skin. in 17 patients with atopic eczema, six epicutaneous test reactions of the delayed type ... | 1986 | 3513815 |
| collection of house dust for analysis of mite allergens. allergen-reducing effect of a self-administered procedure. | to assess allergen-reducing effect of dust collection from mattresses, patients were asked to vacuum clean the entire surface of their mattresses for 5 min with their household cleaners at specified intervals ranging from 1-21 days. ten patients performed four collections, nine patients only two. amounts of dust, concentrations and amounts of major allergens from dermatophagoides pteronyssinus (dp42), dermatophagoides microceras (dm6), and dermatophagoides farinae (df6) were determined. the firs ... | 1986 | 3532859 |
| immunogenicity and tolerogenicity of a major house dust mite allergen, der p i from dermatophagoides pteronyssinus, in mice and rats. | primary immunization of different inbred strains of mice with the house dust mite allergen der p i (from dermatophagoides pteronyssinus) adsorbed to adjuvant revealed differences in ige but not igg responses. thus, the cba and c57 black strains were shown to be high ige responders in that persistent ige was induced which lasted for several months. in contrast, the c3h, akr and balb/c strains were judged to be poor responders. subsequent experiments showed that such responses were adjuvant-indepe ... | 1987 | 3570526 |
| a quantitative enzyme-linked immunosorbent assay for dermatophagoides pteronyssinus-specific immunoglobulin g. | a monoclonal mouse anti-human igg was used to develop an enzyme-linked immunosorbent assay (elisa) for the measurement of dermatophagoides pteronyssinus (dp)-specific igg in human sera. this monoclonal antibody (hg2-25) binds to all subclasses of igg but not to iga, igm, or ige. for the assay, the dp antigen is coated firmly on polystyrene beads through physical adsorption and any leakable antigen is washed off. the assay gives satisfactory reproducibility and parallelism of the dilution curves. ... | 1987 | 3571213 |
| [analysis of allergen from dermatophagoides pteronyssinus (trouessart, 1897) using cock's technic]. | | 1987 | 3574242 |
| allergy indices based on allergen dose-response curve in a randomly selected sample of schoolchildren. | skin-test sensitivity was computed in 61 schoolchildren drawn from a stratified random sample in order to generate two allergy indices based on a quantitative skin-test assay: individual allergy index (iai) and population allergy index (pai). the former to quantitate ige-mediated skin test sensitivity of each subject, the latter to describe the degree of skin reactivity of our sample to a single allergen. five allergen extracts were tested at 4-fold dilutions. sensitivity endpoint data were used ... | 1987 | 3592147 |
| [the significance of mite antigens in kawasaki disease]. | house dust mites have been suspected as a causative agent in kawasaki disease. to assess the role of mite in children with kawasaki disease, we studied 20 cases of kawasaki disease from september 1985 through may 1986. their ages ranged from 2 months to 4 years with a mean of 2.1 years, we assessed intradermal skin test reactivity to mite antigens (dermatophagoides pteronyssinus and dermatophagoides farinae) and anti-mites specific ige (rast) levels in 20 kawasaki disease patients, 10 children f ... | 1987 | 3595263 |
| in vitro translation of messenger rna from the house dust mite dermatophagoides pteronyssinus. | rna was extracted from the house dust mite dermatophagoides pteronyssinus and a polyadenylated [poly(a)+] and non-polyadenylated [poly(a)-] mrna isolated by oligothymidylic acid-cellulose chromatography. both preparations were translated in vitro using a cell-free rabbit reticulocyte system. the relative incorporation of 35s-methionine into trichloroacetic acid-precipitable translation products obtained using poly(a)+ and poly(a)- mrna was 21- and 1.3-fold, respectively, when compared with contr ... | 1987 | 3610352 |
| house dust mites in caracas, venezuela. | an investigation of bedding dust fauna was carried out in caracas, venezuela. mites were recovered from all samples. the average mite density of 15.6 mites/mg dust was among the highest reported worldwide. pyroglyphid mites were the most frequent and abundant, and dermatophagoides pteronyssinus was the largest contributor to the total mite counts. it was also found that the storage mite blomia tropicalis occurred in 96% of the samples examined and contributed an average of 15% of the total mites ... | 1987 | 3619131 |
| vitamin requirements of the european house dust mite, dermatophagoides pteronyssinus (acari: pyroglyphidae), in relation to its fungal association. | | 1987 | 3625716 |
| [effect of mite allergens on the electrophoretic mobility of guinea pig lymphocytes]. | the use of the method of electrophoretic mobility measurement has made it possible to demonstrate that dermatophagoides pteronyssinus and dermatophagoides farinae allergens are species-specific and possess common antigenic properties. the allergenic properties of d. pteronyssinus allergens are more pronounced than those of d. farinae allergens. | 1987 | 3630483 |
| comparison of dermatophagoides pteronyssinus allergens from culture medium extract and whole body extract by using the same probe of pooled human serum. | two distinct allergens of dermatophagoides pteronyssinus were isolated from culture medium extract and whole body extract monitored by the same probe of pooled human serum and compared. ige-binding activity of allergens from the two sources was detected by microplate-elisa with the same probe throughout the column chromatography. an allergen obtained from culture medium extract (ag-cme) had a molecular weight of 27,000 by sds-polyacrylamide gel electrophoresis (page) and two isoelectric point ra ... | 1987 | 3631462 |
| [housedust mites: their importance in the pathogenesis of respiratory allergic diseases in piedmont]. | in this study we have considered the importance of house dust mites (dermatophagoides farinae and pteronyssinus) in the pathogenesis of respiratory allergic diseases in piedmont. we have identified, from 1980 to 1986, 1530 subjects with sensitization to at least one of the tested allergens; 479 of these (31.3%) were allergic to both or to one of the tested mites. particularly 97 subjects (6.3%) were sensitized to dermatophagoides farinae, 73 (4.8%) to dermatophagoides pteronyssinus and 309 (20.2 ... | 1987 | 3658211 |
| preliminary study on the concentration and species composition of bacteria, fungi and mites in samples of house dust from silesia (poland). | twenty-one samples of house dust collected from dwellings situated in upper silesia (poland) were subjected to bacteriological, mycological and acarological examination. moreover, in two samples the concentration of bacterial endotoxin was estimated by the use of limulus test. the mean concentration of bacteria in the examined samples was 2831.9 thousands cfu (colony forming units) per gram, that of fungi - 28.4 thousands cfu per gram, and that of mites - 51.5 specimens per gram. no significant ... | 1987 | 3661356 |
| specific igg antibodies to dermatophagoides pteronyssinus and egg white--their changes with age. | dermatophagoides pteronyssinus-specific and egg white-specific igg in 232 children and 42 adults were measured by the rast method using monoclonal anti-human igg antibodies (hg2-25) as the secondary antibody. changes in allergen-specific igg with the age of the subjects were examined. allergen-specific igg antibody in standard serum was designated as 1,000 u/ml. standard curves were made for each measurement and the levels of allergen-specific igg of the sera were expressed in units (u). dermato ... | 1987 | 3662128 |
| the prevalence of indoor allergens in the baltimore area: house dust-mite and animal-dander antigens measured by immunochemical techniques. | mattress dust samples from 42 homes in baltimore, md., were analyzed by elisa and rocket immunoelectrophoresis (rie) for major excrement allergens (p1 [equal to dp 42], df 6, and dm 6) of house dust mites, dermatophagoides pteronyssinus, d. farinae, and d. microceras, by rie for cat major epithelial allergen (cat ag 1) and horse major epithelial antigen (horse ag 11), and by counter-current immunoelectrophoresis (ccie) for cat and dog antigens. dp 42 was detected by elisa in 24 samples (57%) and ... | 1987 | 3668118 |
| the human immune response against major allergens from house dust mite, dermatophagoides pteronyssinus. ii. idiotypic cross-reactions of allergen-specific antibodies. | antisera were prepared in rabbits against the idiotypic (id) determinants of antiallergen antibodies. these antibodies were isolated from the plasma of 10 unrelated patients by immunoabsorption. three major allergens isolated from the house dust mite, dermatophagoides pteronyssinus (dpt), were used. the anti-id antisera were rendered specific by successive absorption on insolubilized allergens, igg and igm from a pool of human sera and ig from the donor after removal of anti-dpt antibodies by ab ... | 1986 | 3699090 |
| [mites: the principal respiratory allergens of house dust]. | the mites are the most frequent of the lung allergens. three families have a prime importance. the pyroglyphidae (of which dermatophagoides pteronyssinus, dermatophagoides farinae and euroglyphus maynei are a part), the acaridae and the glyciphagidae which comprise the stock acariens responsible for certain occupational asthmas. after the discovery of the role of mites in respiratory allergy numerous studies have specified the ecological conditions favourable to their development (temperature, r ... | 1986 | 3715162 |
| detection of allergens in mould and mite preparations by a nitrocellulose electroblotting technique. | the allergen composition of moulds (alternaria alternata and aspergillus fumigatus) and mites (dermatophagoides pteronyssinus and dermatophagoides farinae) was studied by electroblotting. the allergens were separated by sds-gpage and transferred to a nitrocellulose membrane by electroblotting. specific ige antibodies directed against mould and mite allergens were utilized as probes to detect the allergens. the bands were localized on the nitrocellulose membrane by means of enzyme- or isotope-lab ... | 1986 | 3733230 |
| [detection of mite antigens in house dust in moscow]. | the acarological+ study of dust samples from the homes of allergic patients and normal persons in moscow mite antigens were detected, respectively, in 66.7% and 38.1% of homes. the indirect mast cell degranulation test and the brain gliacyte volume change test in white rats gave similar results. the occurrence of dermatophagoides pteronyssinus was approximately twice as great of d. farinae, and the numerical prevalence of the former species over the latter one was 1.3-fold. dermatophagoides mite ... | 1986 | 3751400 |
| serum ige, skin and radioallergosorbent tests for house dust and mites in asthmatic children. | thirty asthmatic children were given an intradermal skin test, total ige measurements and the radioallergosorbent test (rast) against house dust and house dust mites (dermatophagoides pteronyssinus and dermatophagoides farinae). results of the intradermal skin test and rast were highly positive with overall agreement in 95.6%. the correlation coefficient between the total ige and the rast to house dust was 0.35 (p less than 0.05). there was no significant difference between the total ige and the ... | 1986 | 3771471 |
| serologic aspects of igg4 antibodies. ii. igg4 antibodies form small, nonprecipitating immune complexes due to functional monovalency. | human igg4 antibodies directed against phospholipase a, the p1 antigen from dermatophagoïdes pteronyssinus extracts, and cat albumin were found unable to cross-link antigen. previously, it was demonstrated that igg4 antibodies, in contrast to igg1 antibodies, did not cross-link sepharose-bound antigen and antigen added in solution. to eliminate the possibility that this phenomenon was caused by preferential binding of both igg4 fab fragments to the solid-phase-bound antigen, cross-linking of ant ... | 1986 | 3782791 |
| purification and characterization of a major allergen from the house dust mite dermatophagoides farinae. | a major allergen from an extract of the house dust mite, dermatophagoides farinae, was shown to be extremely heterogenic with respect to charge. a slightly basic component of this allergen with a pi of 8, was purified by isoelectric focusing in two steps. the purified component, denoted antigen 19/20 iia, seemed to be representative for the allergenic activity of the major allergen. the amino acid analysis suggested that antigen 19/20 iia had a molecular weight of 9400 and contained one residue ... | 1986 | 3789326 |
| kawasaki syndrome and house dust mite exposure. | we investigated exposure to house dust mites and freshly cleaned carpets in patients with kawasaki syndrome (ks) who resided in denver, co. house dust samples were analyzed for mites (dermatophagoides pteronyssinus and dermatophagoides farinae) and convalescent sera were assayed for immunoglobulin g antibody to mite antigen. house dust samples from case and neighborhood control homes showed no difference in the prevalence of mites. fewer than 10% of dust samples from either group revealed eviden ... | 1986 | 3797297 |
| demonstration of species-specific sensitization to major allergens of dermatophagoides species by solid-phase absorption of human ige antibodies. | twenty-five mite-allergic patients in the brussels area were tested with the skin-prick test (1, 5 and 10 histamine equivalents by prick test (hep] and rast with standardized extracts of dermatophagoides pteronyssinus (dp) and d. microceras (dm). highly significant correlations (0.69 less than rs less than 0.96) between the two species coincided with marginal, but highly significant (p less than 0.01), greater reactions to dp in all comparisons. species-specific ige antibodies to antigen dp-42 ( ... | 1987 | 3810042 |
| [acari of food storage facilities. an ecologic and immuno-allergic study]. | the authors studied the commonest species of storage mites, living in granaries and in house dust. they found nine species of mites belonging to different systematic groups and were identified: acarus siro, tyrophagus putrescentiae, glycyphagus domesticus, lepidoglyphus destructor, chortoglyphus arcuatus, androlaelaps casalis, cheyletus fortis, cheyletus tenuipilis, thyreophagus entomophagus. skin tests were performed on 14 patients with house dust allergy, using extracts of three storage mites, ... | 1985 | 3837918 |