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immunologic control of a parasitic arthropod. identification of a protective antigen from boophilus microplus.cattle can be vaccinated against the tick boophilus microplus by inducing an immunologic reaction against ag in the tick gut. the uptake of antibody during feeding leads to severe damage to the parasite. one of the responsible tick gut ag has now been purified and characterized: the bm86 ag. it is a membrane-bound glycoprotein present in very low abundance in extracts of partially engorged adult female ticks. it has an apparent m.w. of 89,000, an isoelectric point of 5.1 to 5.6 and an affinity f ...19892745982
histochemical localisation of acid phosphatase and non-specific esterase in the midguts of two species of tick, boophilus microplus and rhipicephalus appendiculatus, as determined by light microscopy.serial sections of glycol methacrylate-embedded and frozen midguts of boophilus microplus and rhipicephalus appendiculatus were studied histochemically by light microscopy. the use of the naphthol as-tr phosphate technique combined with glycol methacrylate embedding enabled the precise localisation of lysosomal enzyme activity, despite the ubiquity of haematin granules in tick midgut epithelia. the presence of acid phosphatase and non-specific esterase activity in the same cells was observed in ...19911724321
detection of babesia bigemina in cattle by a radioimmunoassay incorporating specifically depleted antigen.it was observed that mild acidification (ph less than 4.0) together with solvent extraction of the soluble sonicate of a crude preparation of babesia bigemina infected cattle erythrocytes caused a quantitative loss of b. bigemina-specific antigen. cross-reacting antigen activities with babesia bovis remained intact. these properties were utilized in an assay system wherein antibody response to the specifically depleted antigen preparation was subtracted from the response to the initial crude pre ...19892763443
observation on the composition and biosynthesis of egg wax lipids in the cattle tick, boophilus microplus.the biosynthesis of wax lipids by gené's organ, the egg waxing organ in ticks, was investigated. gené's organ, a complex dermal gland system, applies a superficial wax layer to the eggs during oviposition which prevents desiccation and is essential for egg viability. the detailed anatomy and histology of the three gland cell types are unambiguously described. serial sectioning of ticks showed that all three gland cell types are capable of contributing to the egg wax. the wax synthetic ability of ...19921638927
class i antigens of the bovine major histocompatibility system and resistance to the cattle tick (boophilus microplus) assessed in three different seasons.two consecutive calf crops consisting of 141 three-quarters brahman/one-quarter shorthorn cattle were assessed for resistance to the australian cattle tick boophilus microplus in may, july and october 1983. although the level of expressed resistance to artificial infestation varied considerably between seasons, the animals maintained very similar rankings for resistance in all three seasons, and the repeatability of tick resistance ranged from 0.59 to 0.82. the cattle were typed for 30 bovine cl ...19892763449
efficacy of cyhalothrin and lambdacyhalothrin against boophilus microplus (acari: ixodidae).a study was conducted to determine the efficacy of cyhalothrin and lambdacyhalothrin as a whole-body spray and lambdacyhalothrin as a pour-on application on cattle infested with boophilus microplus (canestrini). a laboratory bioassay was also done with both spray formulations. cattle were infested with all parasitic life stages of b. microplus, then treated with 0.007 or 0.01% (ai) concentrations of cyhalothrin as a spray; 0.005, 0.007, or 0.01% (ai) concentrations of lambdacyhalothrin as a spra ...19921464689
synthetic pyrethroid resistance in field samples in the cattle tick (boophilus microplus).first evidence of resistance to synthetic pyrethroids in the cattle tick in australia, other than the pre-existing cross-resistance in ddt-resistant ticks, has emerged. three new strains have been investigated and characterised. the first, detected in central queensland, showed increased ability to detoxify pyrethroids resulting in a marginal loss of efficacy of all pyrethroid tickicides tested, except flumethrin. subsequently two more strains have been detected, with serious implications for ch ...19892775054
the effect of antigen concentration and vaccine regimen on the immunity induced by membrane antigens from the midgut of boophilus microplus.sheep and cattle were immunized with membrane antigens extracted from the midgut (gm) of the cattle tick boophilus microplus, and antibody levels were measured by enzyme-linked immunosorbent assay (elisa). one microgram of gm induced an antibody response in sheep comparable to that induced by 500 micrograms gm. single or divided doses of 500 micrograms gm induced the same antibody levels in sheep over 12 weeks. cattle vaccinated with either 500 micrograms gm in two doses or with 50 or 500 microg ...19892807382
effect of age on cholinesterase activity and protein of unfed larval ticks.the cholinesterase (ache) activity and total protein in homogenates of unfed larvae of amblyomma americanum (l.), a. cajennense (fabricius), a. maculatum koch, anocentor nitens neumann, and boophilus microplus (canestrini) were determined weekly in ticks 1-6 wk of age. there was a considerable variation in total protein, ache, and the ratio of ache to protein as the ticks aged. however, ache activities were constant or increased with age and total protein levels were constant or decreased. no di ...19892918444
identification of ecdysone 22-long-chain fatty acyl esters in newly laid eggs of the cattle tick boophilus microplus.the five major apolar ecdysone esters present in newly laid eggs of the cattle tick boophilus microplus have been purified by h.p.l.c. the quantities of the apolar esters present in the eggs were increased by administration of ecdysone to the mature females. g.c.-m.s. analysis, as their methyl esters, of the fatty acids released from the apolar ecdysone derivatives by alkali, coupled with positive-ion fast-atom-bombardment m.s. of the intact ecdysone esters, showed that the compounds consisted o ...19863030275
epidemiology of bovine babesiosis and anaplasmosis in zambia.the serological prevalence of bovine babesiosis and anaplasmosis in the traditional farming sector of six provinces of zambia was determined using the indirect fluorescent antibody test (ifat) for babesiosis and the card agglutination test (cat) for anaplasmosis. antibodies to babesia bigemina occurred throughout the country whereas the prevalence of b. bovis followed the distribution of its tick vector boophilus microplus which is limited to the north-eastern part of the country. low numbers of ...19883070875
hereford cattle immunized and protected against boophilus microplus with soluble and membrane-associated antigens from the midgut of ticks.hereford cattle were immunized with membranes and soluble components extracted from the midgut of boophilus microplus. membrane vaccines protected cattle (91%) against challenge with 3 x 20,000 larval ticks administered at intervals of 7 days. vaccines made from soluble antigens did not protect cattle. antibody levels measured by enzyme-linked immunosorbent assay (elisa) related to the levels of protection induced by vaccination.19883174172
effect of temperature on the ovipositional biology and egg viability of the cattle tick boophilus annulatus (acari: ixodidae).the effect of temperature on the ovipositional biology of boophilus annulatus (say) was determined under laboratory conditions. engorged females subjected to constant temperatures of 12 and 45 degrees c died without ovipositing, while females held at 15 and 40 degrees c laid eggs which did not hatch. the preoviposition period at 25-40 degrees c was 2-3 days; however, significant increases occurred at 20 degrees c (5.2 days) and at 15 degrees c (16.3 days). the number of eggs laid per female was ...19883197576
a survey of cattle tick control practices in the eastern cape province of south africa.current cattle tick control practices and producer attitudes towards tick control in the eastern cape province of south africa are discussed. these were ascertained from answers to a questionnaire survey to which 31.2% of farmers responded. in general, producers favoured intensive tick control. beef and dairy farmers had a definite preference for synthetic pyrethroid acaricides, the majority followed a 25 times p.a. treatment frequency and most changed acaricides because of price. beef producers ...19921437023
a technique for collecting saliva from the cattle-tick boophilus microplus (canestrini, 1887) using chemical stimulation. environmental and temporal influences on secretion yield.1. injection of a pilocarpine solution into the hemocoele of female b. microplus through the respiratory spiracle induced the flow of limpid saliva, collected from the mouth parts with a capillary tube. 2. the time interval between the detachment of the tick from its host and chemical stimulation influenced the volume of saliva secreted; secretion was greater during the first 2 h after detachment. 3. there is a positive correlation between salivary yield and both environmental temperature and re ...19883228628
characterization of tick antigens inducing host immune resistance. ii. description of rabbit-acquired immunity to amblyomma americanum ticks and identification of potential tick antigens by western blot analysis.feeding by adult amblyomma americanum ticks induced a level of immunity in rabbits to subsequent tick feeding that resulted in a significant decrease in tick feeding success and fecundity. histological analysis of tick feeding sites in hosts expressing resistance revealed a predominant eosinophil response, with weak basophil and neutrophil infiltrates. while the basophil was never the dominant granulocyte at the tick feeding sites in resistant hosts, this cell exhibited the greatest increase in ...19883291384
ticks (acari:ixodidae) of the blue and white nile ecosystems in the sudan with particular reference to the rhipicephalus sanguineus group.twenty-four adult ixodid tick species, infesting livestock and some wildlife hosts along the blue and white nile in the sudan, were identified. three species, boophilus geigyi, rhipicephalus camicasi and r. bergeoni, were recorded for the first time from the sudan. tick numbers on indigenous breeds of cattle (bos indicus) were relatively low, ranging between 17.1 and 40.5 per animal. young cattle grazing with the herd carried significantly fewer ticks than older animals. with the exception of b. ...19873331134
histochemical changes in the midgut of two ixodid tick species boophilus microplus and rhipicephalus appendiculatus during digestion of the blood meal.the changes in the midgut epithelia of two ixodid tick species, boophilus microplus and rhipicephalus appendiculatus, have been studied using several histochemical techniques. it was revealed that there is an accumulation of rna at the time of tick attachment to the host and prior to the arrival of the blood meal, indicating that the midgut digest cell is furnished with the machinery characteristic of a synthetic cell. there appears to be a synchrony in the appearance of granules with peroxidase ...19921373357
ticks parasitising the indian buffalo (bubalus bubalis) and their possible role in disease transmission.a total of 13 ixodid tick species, boophilus microplus, haemaphysalis bispinosa, haemaphysalis cornupunctata, haemaphysalis himalaya, heamaphysalis montgomeryi, hyalomma anatolicum anatolicum, hyalomma dromedarii, hyalomma marginatum isaaci, hyalomma (hyalommina) brevipunctata, hyalomma (hyalommina) hussaini, nosomma monstrosum, rhipicephalus haemaphysaloides, and r. turanicus were collected off 424 buffaloes from the northwestern states of india. ten tick species, amblyomma testudinarium, b. mi ...19883369083
isolation from the cattle tick, boophilus microplus, of antigenic material capable of eliciting a protective immunological response in the bovine host. 19883372125
selection for resistance to coumaphos in a strain of southern cattle tick (acari: ixodidae). 19883372854
radioimmunoassay for anaplasma marginale antibodies in cattle.a radioimmunoassay is described for use in the detection of anaplasma marginale antibodies in cattle sera. optimal sensitivity and specificity were obtained by using 2 antigens, an a marginale antigen and a rbc antigen (obtained before infection was established) from the same calf. in addition, sera were preabsorbed with rbc from healthy cattle and with sonicated babesia bovis. of 86 sera obtained from cattle with a marginale infection (as determined by blood smear examination or by results of s ...19883377309
kinetic properties of toxic protease inhibitors isolated from tick eggs.1. egg-toxins from rhipicephalus evertsi evertsi, boophilus microplus, boophilus decoloratus and hyalomma truncatum were found to be inhibitors of trypsin and in two cases also of chymotrypsin. 2. fast tight-binding and slow-binding inhibition were observed. 3. immunological identity of the toxins were assessed with ouchterlony immunodiffusion and elisa. 4. the protease content of b. decoloratus and amblyomma hebraeum tick eggs were determined by a linked enzyme assay. 5. the predictive value of ...19883391338
persistent activity of injectable ivermectin in the control of the cattle tick boophilus microplus. 19883407118
successful vaccination against boophilus microplus and babesia bovis using recombinant antigens.current methods for the control of the cattle tick boophilus microplus and the agent of bovine babesiosis, babesia bovis are unsatisfactory. effective immunological control of both parasites would have great advantages. however, naturally acquired immunity to the tick is generally unable to prevent serious production losses. a vaccine against the tick, based on a novel form of immunization, is being developed. a protective antigen has been isolated from the tick, characterized and produced as an ...19921343705
comparative study of tick burdens in gudali and wakwa cattle under natural infestation in the subhumid highlands of wakwa, cameroon. preliminary observations.the relative resistance to different cattle ticks of gudali and wakwa cattle with different levels of brahman breeding, grazed on natural pastures in the subhumid tropics of wakwa, cameroon, was assessed using pasture tick infestations. the basic design consisted of 5 young bulls of each breed from different sire herds. tick populations were observed in gudali and wakwa bulls over a period of four weeks when the climate was thought to be highly favourable for the free-living stages of ticks. cou ...19921339999
[efficacy of chlorfenvinphos in cattle-tick control in the north-western cameroon]. 19873444970
the effect of imidocarb treatment on babesia in the bovine and the tick (boophilus microplus).treatment of calves with 5 mg/kg imidocarb (3,3-1-bis-(2 - imidazolin - 2 - yl)carbanilide dipropionate) given intramuscularly 14 days before and 14 days after exposure to babesia infected boophilus microplus larvae rendered the next generation of larvae incapable of transmitting babesia infection. when administered to calves 14 or 28 days before tick exposure, the drug prevented the development of clinical babesiosis; the larval progeny of ticks reared on the calf which was treated 28 days bef ...19751129539
the effects of precocene treatment on egg wax production in gené's organ and egg viability in the cattle tick boophilus microplus (acarina ixodidae): an ultrastructural study.wax synthesis by gené's organ, the egg waxing organ of ticks, is by precocene treatment of engorged female boophilus microplus, resulting in desiccated nonviable eggs due to the absence of a waterproofing wax layer. electron microscopy shows that precocene has a destructive effect on the glandular cells of gené's organ. the precocene also inhibited in vitro wax synthesis by the gland cells, indicating a selective cytotoxic effect. oogenesis and oviposition were otherwise unaffected, also suggest ...19863451863
tick transmission of anaplasma centrale.anaplasma centrale was isolated from a field collection of rhipicephalus simus. transstadial transmission of a. centrale with adult ticks was demonstrated, but the infection was not carried transovarially. ticks from this collection were subsequently reared as a non-infected, laboratory strain. it was proved that the onderstepoort live blood vaccine strain of a. centrale, isolated by theiler in 1911, is still tick transmissible after more than 75 years of needle passage through cattle in the lab ...19873587927
ultrastructure of secretory cells in the gut of the cattle-tick boophilus microplus. 19873654050
control of cattle ticks using flumethrin in central zambia. 19873660539
control of cattle ticks. 19873660566
metabolism of ecdysteroids in the female tick amblyomma hebraeum (ixodoidea, ixodidae): accumulation of free ecdysone and 20-hydroxyecdysone in the eggs.[3h]-20-hydroxyecdysone ([3h]-20e) injected into amblyomma hebraeum females 7 days before the beginning of oviposition, viz. at the beginning of vitellogenesis, was converted to 3 polar peaks of unknown nature called 1, 2 and 3, and to apolar conjugates ap1, ap2 and ap3. ap2 have the same retention times as the esters of 20e with long chain fatty acids described in ornithodoros moubata (diehl et al. 1985). however, principally unmetabolized 20e was incorporated into the ovaries, and 16% of the i ...19873693624
immunization of cattle against boophilus microplus using extracts derived from adult female ticks: effects of induced immunity on tick populations. 19863699973
immunization of cattle against boophilus microplus using extracts derived from adult female ticks: histopathology of ticks feeding on vaccinated cattle. 19863699974
effect of different methods of maintenance on the development and morphology of babesia bigemina in the gut of boophilus microplus.the development and morphology of babesia bigemina in the gut of boophilus microplus ticks were studied during laboratory maintenance of the babesia by four methods: tick transmission in unsplenectomised (intact) calves; tick transmission in splenectomised calves; syringe passage at intervals of five days or less in splenectomised calves; and syringe passage at intervals of six weeks or more in intact calves. the first method had no apparent effect on the development of b bigemina in ticks compa ...19863704330
genetic similarity and variability between natural populations and laboratory colonies of north american boophilus (acari: ixodidae).four natural populations and 4 laboratory colonies of boophilus microplus (canestrini) and a laboratory colony of boophilus annulatus (say) were subjected to electrophoretic analysis to determine levels of genetic similarity and genetic variability. populations of b. microplus exhibited high genetic similarity (i = 0.984 +/- 0.012) indicating that all populations share a relatively undifferentiated gene pool. host vagility is proposed as an important mechanism promoting gene flow in ectoparasite ...19863712179
boophilus microplus: cellular responses to larval attachment and their relationship to host resistance.the histology of early feeding lesions of the cattle tick b. microplus has been studied using 32p labelled larvae to standardize the duration of attachment. critical studies were made on 3-h lesions in six separate experiments on different groups of british breed animals. each group consisted of three animals--one previously unexposed to ticks, one of high resistance and one of low resistance. the degree of mast cell disruption, eosinophil concentration and degranulation, and the extent of epide ...19761023863
boophilus microplus: characterization of enzymes introduced into the host.a number of enzymes, presumably secreted by larvae of b. microplus under natural feeding conditions, have been investigated in the skin of previously unexposed calves 4 h after infestation at the attachment site. carboxylic ester hydrolase activity was demonstrated in the dermis, immediately adjacent to the mouthparts, or in the attachment cone, depending on substrate and reaction ph. the carboxylic ester hydrolase acting on naphthol as-d acetate (2-acetoxy-3-naphthoic-o-toluidide) at ph 7-1 was ...19761023862
immune responses of cattle to biochemically modified antigens from the midgut of the cattle tick, boophilus microplus.treatment of membrane antigens of the midgut (gm) of the cattle tick, boophilus microplus with sodium metaperiodate (periodate), pronase and lipase significantly inhibited the reactivity of the gm with antibodies in the sera of 57 cattle vaccinated with gm. treatment of gm with periodate only removed the correlation between antibody reactivity of sera and protection against infestation with ticks. a monoclonal antibody (moab qu13), which recognises protective antigens solubilized from gm (lee + ...19911811216
a microplate enzyme-linked immunosorbent assay for measuring antibody to anaplasma marginale in cattle serum.an enzyme-linked immunosorbent assay (elisa) method is described for measuring antibody against anaplasma marginale in cattle serum. this method was more sensitive and objective than a previously described elisa method for a. marginale and possible reasons for this are discussed. all 83 cattle experimentally infected with a. marginale (81) or a. centrale (2) developed demonstrable specific antibody but the serums of 98.8% of 839 cattle from cattle tick-free areas did not react by elisa; 378 seru ...19863729834
the inability of a south african babesia bovis vaccine strain to infect boophilus microplus.a strain of babesia bovis that had been attenuated by rapid syringe passage through a series of 23 splenectomized calves was unable to infect its vector boophilus microplus. an attempt to transmit the attenuated australian babesia bigemina g strain with a south african strain of b. microplus was likewise unsuccessful. the epidemiological implication of these observations in terms of babesiosis control is discussed.19863763166
continued functioning of the feeding apparatus during moulting of boophilus microplus as an adaptation of one-host ticks.nymphs of the 1-host cattle tick boophilus microplus remain attached to the host during moulting, but this process is not uniform throughout the tick tissues. the muscles of the pharynx and salivarium remain attached to the cuticle and are functional while the cuticle of the legs has already separated from the underlying epidermis and muscle. the nymphs continue to feed for 2 days after the moulting process in the legs has begun, and they more than double their weight in this time. mouth-part pr ...19863819961
effect of age of boophilus microplus larvae (acari: ixodidae) on attachment to cattle. 19873820234
observations on the efficacy of ivermectin in the control of cattle ticks in zambia.the efficacy of multiple subcutaneous injections (200 micrograms/kg) of ivermectin in the control of naturally occurring tick infestations on traditionally managed tonga-iia (sanga type) calves and yearlings was assessed in two field trials. in the first trial boophilus decoloratus infestations were decreased following treatments at monthly intervals. in the second trial, with weekly and two weekly treatment intervals, infestations of rhipicephalus appendiculatus were controlled less effectively ...19853841240
the effect of babesia bigemina infections caused by cattle ticks on nigerian economy. 19853841960
cross-reaction of tick salivary antigens in the boophilus microplus-cattle system.calves were immunized with boophilus microplus saliva, filtered through millipore membranes, in freund's complete adjuvant. serum samples were tested by passive hemagglutination against babesia bigemina, anaplasma marginale, b. microplus larvae extract, stomoxys calcitrans extract and b. microplus saliva. after immunization, titers to saliva, larval tick-extract and to s. calcitrans were increased. the challenge with live tick larvae enhanced the formation of antibodies against larva extract, fl ...19853992881
non-random mating between boophilus microplus and hybrids of b. microplus females and b. annulatus males, and its possible effect on sterile male hybrid control releases.when boophilus microplus and type-ii hybrids (b. microplus females x b. annulatus males) were released simultaneously onto bovine hosts, mating between the two forms appeared not to be at random. there were more contypic and fewer intertypic matings than predicted under an assumption of panmixia. an examination of the patterns of matings revealed that more of the matings on the first two days of detachment were between the two sexes of b. microplus. engorged females dropping on the last four day ...19911860415
field efficacy of ear tags impregnated with insecticide against the cattle tick boophilus microplus. 19854004653
borrelia theileri: isolation from ticks (boophilus microplus) and tick-borne transmission between splenectomized calves.the bovine spirochete, borrelia theileri, was detected in giemsastained blood smears from a splenectomized calf 17 days after exposure to a laboratory colony of the tropical cattle tick, boophilus microplus. spirochetes were detected in the hemolymph and ovary of all engorged female ticks examined, indicating a high infection rate in this tick colony. spirochetes were detected in a 2nd splenectomized calf 15 days after exposure to the larval offspring of ticks from the 1st calf. the only observa ...19854026019
ixodes (ixodes) pararicinus, n. sp. (acari: ixodoidea: ixodidae), a south american cattle tick long confused with ixodes ricinus. 19854045937
predation of ticks of the rhipicephalus sanguineus (latreille, 1806) group and boophilus microplus (canestrini, 1888) (acari: ixodidae) by nothura temminck, 1815 (aves: tinamidae) in salta, argentina. 19911937269
relationship between the resistance of cattle to haemaphysalis longicornis and to boophilus microplus.cattle that had been exposed to haemaphysalis longicornis were as susceptible to boophilus microplus as cattle that had never been exposed to either species of tick. cattle with acquired resistance to both species ranked consistently for levels of resistance to each when infested separately. concurrent infestation with h. longicornis had no effect on ranking for resistance to b. microplus. the coefficient of concordance between the rankings of individuals on their levels of resistance to both sp ...19854074218
ticks on livestock in st. lucia.cattle, sheep, goats and horses were examined for ticks. over 95% of holstein cross-breeds, 28% of sheep (local mixed breeds) and 18% of goats (local mixed breeds) examined from 18 august to 4 september 1983 were infested with the southern cattle tick, boophilus microplus canestrini. about 90 and 17% of the horses examined were infested with the tropical horse tick, anocentor nitens neumann, and the tropical bont tick, amblyomma variegatum fabricius, respectively. the tropical bont tick was foun ...19854090245
characterization of the organophosphorus-resistant mt alford, gracemere and silkwood strains of the cattle tick, boophilus microplus. 19744139946
enzyme activity of the cytochrome system of the egg and larva of the cattle tick (boophilus microplus). 19734146220
detection of trypanosoma theileri in ethiopian cattle ticks. 19734149680
boophilus microplus: antihistaminic and tranquillizing drugs and cattle resistance. 19694396008
boophilus microplus: antihistaminic and tranquilizing drugs and cattle resistance. 19694396213
the transmission of babesia bovis using frozen infective material obtained from boophilus microplus larvae. 19744449642
control of the southern cattle tick by pour-on animal systemic insecticides. 19724639456
transmission of anaplasma marginale by the cattle tick boophilus microplus. 19724640755
preliminary studies on the transmission of anaplasma marginale by boophilus microplus. 19734689814
bioassay of chlorphenamidine using larvae of the cattle tick boophilus microplus. 19734711148
inheritance of dimethoate resistance in the mackay strain of the cattle tick (boophilus microplus) in australia. 19734717730
toxicity to boophilus microplus of formamidine acaricides and related compounds, and modification of toxicity by certain insecticide synergists. 19734770945
boophilus microplus: digestion of hemoglobins by the engorged female tick. 19744815010
babesia argentina: the infection of splenectomized calves with extracts of larval ticks (boophilus microplus). 19744819982
acaricide ac 84,633: first trials for control of boophilus microplus. 19744839224
toxicity of piperonyl butoxide to boophilus microplus. 19744858271
cattle tick in new south wales. 19694936858
loss of infectivity of a vaccine strain of babesia argentina for boophilus microplus. 19664961183
the infection of boophilus microplus with babesia bigemina. 19684984380
[fine structure of the merozoites of babesia bigemina in the ovary of boophilus microplus and boophilus decoloratus]. 19694988938
bovine babesiasis: estimation of infection rates in the tick vector boophilus microplus (canestrini). 19714999518
sulphur and the cattle tick boophilus microplus. 19725013812
electrical studies on the feeding of the cattle-tick, boophilus microplus. 19725015014
the inheritance of resistance by cattle to cattle tick. 19725068812
behavior of larvae of the cattle tick, boophilus microplus (canestrini), on cattle of differing degrees of resistance. 19715090974
a modification of pasture spelling to reduce acaricide treatments for cattle tick control. 19715103591
factors affecting the distribution and abundance of the cattle tick in australia: observations and hypotheses. 19715105775
the effect of cattle tick (boophilus microplus) on pregnant and lactating brahman-cross and british-breed cows in northern australia. 19715106528
the acquired resistance to babesia argentina of cattle exposed to light infestation with cattle tick (boophilus microplus). 19715106905
the effect of cattle tick (boophilus microplus) on beef production in the northern territory. 19715106909
isolation of protective antigens from the gut of boophilus microplus using monoclonal antibodies.monoclonal antibodies (mab) were produced against midgut membrane (gm) antigens of boophilus microplus. the isotypes of these mab were established and their specificity characterized using double diffusion and western blotting. gm antigens solubilized by triton x-100 were precipitated by mab qu13, and the precipitate was then injected into cattle to test for the presence of protective antigens. vaccinated cattle challenged with 10-day-old larval ticks showed evidence of protection with a 62% red ...19911997395
relationship between the resistance of crossbred cattle to ticks, boophilus microplus (canestrini, 1887) and hyalomma anatolicum anatolicum (koch, 1844).studies were undertaken to determine the effect of repeated pure infestations with boophilus microplus on susceptibility to subsequent pure infestations with hyalomma anatolicum anatolicum, and the effects of pure infestations with both species of tick on susceptibility to a series of mixed infestations. crossbred (bos indicus x bos taurus) calves were infested with boophilus microplus (17 times), h. a. anatolicum (four times), followed by five mixed infestations of b. microplus and h. a. anatol ...19892763448
formulation of medium for tick cell culture.we examined the effectiveness of bovine cholesterol concentrate in reducing the high level (10-20%) of fetal bovine serum (fbs) necessary to promote tick cell growth in vitro. tick cell lines isolated from embryos of anocentor nitens (ane 58), boophilus microplus (bme 26), and rhipicephalus appendiculatus (rae 25) were used. they were incubated in l-15 (bme 26) or l-15b (ane 58 and rae 25) supplemented with 10% tryptose phosphate broth (tpb), 5% (ane 58 and bme 26) or 3% fbs, 10-90 microns/ml ch ...19892766897
efficacy of topically applied ivermectin against boophilus microplus (canestrini, 1887) in cattle.systemic efficacy of ivermectin applied topically was evaluated against the cattle tick, boophilus microplus. twenty cattle with induced infestations were randomly allocated to five groups of equal size based on the numbers of engorged female ticks which fell through the slatted floor of individual pens during the 3 days prior to treatments. control cattle were non-medicated. cattle in three groups were given ivermectin at 200, 500 or 1000 mcg kg-1 in a topical formulation applied along the back ...19883201709
vaccines to protect hereford cattle against the cattle tick, boophilus microplus.vaccines made from gut and gut and synganglion tissue dissected from boophilus microplus gave 87% and 80% protection, respectively, compared with adjuvant-injected controls in cattle against three infestations with 20,000 larval ticks administered over 14 days. a vaccine prepared from synganglion alone did not protect cattle. ticks collected from vaccinated cattle produced 95% and 91% fewer eggs, respectively, than ticks from control animals. vaccinated cattle were protected (36%) 7 months after ...19883350577
effect of mercury vapour on the non-protein thiol content and respiration rate of eggs of the cattle tick boophilus microplus (acari: ixodidae).with continuous and ultimately lethal exposure of eggs of boophilus microplus to mercury vapour, respiration and non-protein thiol decreased to low levels. with sub-lethal exposure, respiration decreased initially and then increased, whereas non-protein thiol (largely glutathione) increased initially and then decreased. this initial increase in glutathione (gsh) which probably resulted from an attempt by the organism to control or reverse the biochemical lesion, did not arise from the oxidized f ...19883409788
flumethrin applied as a pour-on and whole-body spray for controlling cattle tick (acari: ixodidae) on cattle. 19883417920
boophilus microplus: passage of bovine immunoglobulins and albumin across the gut of cattle ticks feeding on normal or vaccinated cattle.cattle were vaccinated with antigens from adult female boophilus microplus and haemolymph was collected from female ticks which had engorged on these animals and on matched control cattle. radio-immunoassay for bovine plasma proteins in haemolymph from ticks fed on control cattle showed low concentrations of igg1 and albumin. there was a significant increase in bovine plasma proteins passing across the gut in ticks fed on vaccinated cattle, with an average of 150 times more albumin and four to f ...19873444975
babesia bovis infection of secretory cells in the gut of the vector tick boophilus microplus. 19863721701
immunization of cattle against boophilus microplus using extracts derived from adult female ticks: feeding and survival of the parasite on vaccinated cattle. 19863721702
bovine babesiosis (babesia bovis infection) in zambia.two cases of babesia bovis, a parasite associated with the tick boophilus microplus, are reported for the first time from the central part of zambia. it is concluded that infected b. microplus ticks are occasionally introduced into central zambia by tick-infested cattle from the north-eastern part of the country where b. bovis is endemic. the spread of b. microplus in southern africa in a westward direction is discussed and related to the epidemiology of bovine babesiosis in zambia.19863727339
new records for rhipicephalus bursa, boophilus microplus, b.decoloratus and hypoderma lineatum from libya. 19911768917
digestion in the cattle-tick boophilus microplus: light microscope study of the gut cells in nymphs and females. 19853997347
changes in cattle tick control practices in south eastern queensland from 1977 to 1982.results of a survey of cattle producers in south eastern queensland in 1982 concerning cattle tick control have been compared with those of a similar survey carried out in 1977-78. during the interval between these surveys the proportion of beef farms with tick resistant (bos indicus infusion) cattle as the main breed had increased from 47.8% to 60.0%. chemical control methods had improved but many producers were continuing to treat their cattle more often than necessary. over the period there w ...19854051912
larval membrane antigens protect hereford cattle against infestation with boophilus microplus.hereford cattle (bos taurus) were immunized with antigens solubilized with triton x-100 from larval membranes of the cattle tick (boophilus microplus). based on tick egg production compared to control cattle, vaccinated cattle were protected (78%) against challenge with 2 x 20,000 tick larvae. the soluble triton x-100 extract of tick larval membranes was further purified by immunoaffinity chromatography, using immunoglobulin ligands (igg1 and igg2) from three immune steers, previously vaccinated ...19902314923
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