| carbohydrates in the guinea pig stria vascularis demonstrated with lectin-gold techniques. | soybean agglutinin (sba), helix pomatia agglutinin (hpa), ricinus communis agglutinin-ii (rca-ii), limax flavus agglutinin (lfa) and wheat germ agglutinin (wga) were employed to determine the localization of specific carbohydrates on thin sections of lowicryl k4m embedded guinea pig striae vasculares using the lectin-gold and glycoprotein-gold techniques. sba, hpa and rca-ii gold labeling was observed in many of the cytoplasmic vesicles, endosomes and apical tubules located in the supranuclear r ... | 1991 | 1763624 |
| use of thermospray liquid chromatography-mass spectrometry to aid in the identification of urinary metabolites of a novel antiepileptic drug, lamotrigine. | the use of thermospray liquid chromatography-mass spectrometry allowed the structural elucidation of a number of urinary metabolites of lamotrigine, 3,5-diamino-6-(2,3-dichlorophenyl)-1,2,4-triazine, formed after administering the drug to man, cynomolgus monkey and rabbit. this data when combined with the data obtained from high-performance liquid chromatography with radiochemical detection enabled us to determine the types and amounts of unchanged drug and metabolites excreted in urine by man a ... | 1991 | 1795036 |
| biochemical and serological properties of streptococcus uberis. | the strep-zym identification system, a combination of 23 enzymatic tests, allowed a rapid biochemical characterization of streptococcus uberis. the biochemical profiles of the s. uberis cultures clearly differed from those of s. agalactiae and s. dysgalactiae. serological grouping of s. uberis revealed polysaccharide antigens of groups e, g, p and u. some cultures of s. uberis demonstrated camp-like synergistic hemolytic activities on sheep blood agar and reacted specifically with the lectins of ... | 1991 | 1796686 |
| [how thick is the glycocalyx of human erythrocytes?]. | the aim of the experiment was to determine the thickness of the glycocalyx of human erythrocytes of the blood group a. for that purpose, the distance between the electron dense contrasted lipid layer of the plasmalemma and gold sol particles loaded with helix pomatia lectin was determined. the mean thickness of the glycocalyx under this conditions was 5.9 nm. | 1991 | 1801510 |
| defining the specificity of anti-a/b igm/g antibodies with different antigens and lectins. | the specificity of anti-histo blood group a/b antibodies is defined by the antigen used for their production and selection (in the case of mouse monoclonal antibodies, mabs) or by the antigens present on various intestinal bacteria (for polyclonal human igm and/or igg, phabs). absorption experiments with red blood cells and free antigen have been used earlier to define specificity; here we use a/b substances of human, animal and synthetic origin as well as known reactivities of mabs or lectins a ... | 1991 | 1862641 |
| a 58-kda resident protein of the cis golgi cisterna is not terminally glycosylated. | a 58-kda golgi protein (gp58) was previously identified and found to be concentrated in cis golgi cisternae in several cell types (saraste, j., palade, g.e., and farquhar, m.g. (1987) j. cell biol. 105, 2021-2029). in this study the protein was partially purified from rat pancreas and mouse myeloma cells in order to characterize its oligosaccharides. it migrated on sodium dodecyl sulfate-polyacrylamide gels as a 57-58-kda doublet under reducing conditions or as a single approximately 116-kda ban ... | 1991 | 1894639 |
| biosynthesis in vitro of glca beta 1-3nlcose4cer by a novel glucuronyltransferase (glcat-1) from embryonic chicken brain. | a novel glucuronyltransferase (glcat-1) has been detected in embryonic chicken brains. this enzyme catalyzes the biosynthesis in vitro of glucuronic acid containing glycolipids starting from neolactotetraosylceramide (nlcose4cer) and neolactohexaosylceramide (nlcose6cer). the activity is present primarily in the golgi-rich membrane fraction and can be extracted (60%) from the membrane using a neutral detergent, nonidet p-40, at ph 7.0. the detergent-solubilized glcat-1 is stable (70%) at -20 deg ... | 1991 | 1900517 |
| glycoconjugates in the human fetal endolymphatic sac as detected by lectins. | the distribution of glycoconjugates in the 11 to 16 weeks old human fetal endolymphatic sac (es) was analyzed using six biotinylated lectins; wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). in the 11 week old human fetus, fluorescent reactions with wga, apa, rca120 and cona were detected in the es. there was almost no reaction with hpa and uea-i. ... | 1991 | 1919336 |
| species identification and characterization of an acanthamoeba strain from human cornea. | the isoenzyme pattern of an acanthamoeba, stock h-1, isolated from a patient with keratitis (krankenhaus heidberg, hamburg) was compared with that of two strains of a. quina-a. lugdunensis (302-2, 312-1), two stocks of a. lenticulata (45, 89-1) and one strain of a. rhysodes (302-1). the isolated stock showed glucose-6-phosphate dehydrogenase (g-6-pdh), beta-hydroxybutyric dehydrogenase (beta-hbdh), alcohol dehydrogenase (adh) and superoxide dismutase (sod) isoenzyme patterns similar to those of ... | 1991 | 1924251 |
| decreased biosynthesis of forssman glycolipid after retinoic acid-induced differentiation of mouse f9 teratocarcinoma cells. lectin-affinity chromatography of the glycolipid-derived oligosaccharide. | glycolipids synthesized by the mouse teratocarcinoma f9 cells and f9 cells (ra/f9 cells) induced to differentiate by a 3-day treatment with 0.1 microm all-trans-retinoic acid were analyzed. both f9 cells and ra/f9 cells were incubated in media containing either d-[6-3h]galactose or d-[6-3h]glucosamine; the metabolically-radiolabeled glycolipids were isolated and the oligosaccharides were released from the glycolipids by ozonolysis and alkali fragmentation. from both cells, a single major pentasa ... | 1991 | 1933935 |
| metabolism and fate of ecdysteroids in the nematodes ascaris suum and parascaris equorum. | when injected with [3h]ecdysone and maintained in vitro, the parasitic nematodes, ascaris suum and parascaris equorum each produced a series of polar and relatively apolar metabolites. a. suum metabolised the compound into ecdysonoic acid ([3h]eoic), ecdysone 25-glucoside ([3h]e25gluc), putative ecdysone 22-phosphate ([3h]e22p) and a series of at least six relatively apolar metabolites. all of these, except ecdysonoic acid, were hydrolysed by a crude enzyme preparation from helix pomatia, releas ... | 1991 | 1944416 |
| aluminofluorides and beryllofluorides as inhibitors of sulphatases. analogues of hydrogen sulphate? | the inhibition of fluoride of sulphatase a from ox liver and of the sulphatases of helix pomatia and aspergillus oryzae is decreased by edta and increased by al3+ or be2+, implicating aluminofluorides and beryllofluorides in the reaction. the inhibition, which is reversible, takes several minutes to develop fully and, at least for the sulphatase of h. pomatia, is of a non-linear mixed competitive-non-competitive type. it is suggested that the aluminofluorides and beryllofluorides are acting as a ... | 1991 | 1953634 |
| the adsorption of bacteria to immobilized lectins. | the agglutination of a selection of bacteria by some lectins was examined. the lectin from codium fragile agglutinated seven strains of salmonella typhimurium. the lectin from helix pomatia agglutinated eight of 12 strains of listeria monocytogenes and a further two strains gave a weak agglutination reaction. helix pomatia lectin conjugated to magnetic microspheres enabled the adsorption of l. monocytogenes from suspension with subsequent elution by the competing ligand n-acetyl galactosamine. a ... | 1991 | 1955420 |
| expression of blood group-related antigens and helix pomatia agglutinin in malignant pleural mesothelioma and pulmonary adenocarcinoma. | in an attempt to differentiate malignant pleural mesothelioma from pulmonary adenocarcinoma by histochemical and immunohistochemical means, the glycoconjugate profiles of five reactive mesothelial lesions, 29 mesotheliomas (20 epithelial, three biphasic, and six fibrous types), and 38 well-differentiated pulmonary adenocarcinomas (34 papillary, two tubular, and two bronchioloalveolar types) were tested with abh blood group-related antigens (bgr-ag) antibody and helix pomatia agglutinin (hpa) whi ... | 1991 | 2001875 |
| binding of platelet agglutinating protein p37 from the plasma of a patient with thrombotic thrombocytopenic purpura to human platelets. | we have previously reported the purification of a 37 kda platelet agglutinating protein (pap p37) from the plasma of a patient with thrombotic thrombocytopenic purpura (ttp) that was shown to be present in a subset of ttp patients. to gain further insight into the interaction between pap p37 and platelets, we have studied the properties of pap p37 binding to platelets. washed human platelets from two normal donors and two ttp patients after recovery were used for the experiments. the pap p37 bin ... | 1991 | 2024244 |
| lectin histochemistry of developing submandibular glands of fetal syrian golden hamsters. | lectin binding was studied in the developing submandibular glands of fetal syrian golden hamsters (mesocricetus auratus) from gestational day 12 to 16 (the day of birth). the fetuses were fixed, embedded in paraffin, sectioned and stained with nine lectin-horseradish peroxidase conjugates: concanavalin a (con a), wheat germ agglutinin (wga), dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), maclura pomifera agglutinin (mpa). griffonia simplicifolia agglutinin i-b4 (gsa i-b4), p ... | 1991 | 2035849 |
| glycoconjugate distribution in the human fundic mucosa revealed by lectin- and glycoprotein-gold cytochemistry. | the glycoconjugates of the human fundic mucosa were characterized at the ultrastructural level by means of direct (helix pomatia agglutinin-gold complex) and indirect lectin techniques (concanavalin a and horseradish peroxidase-gold complex; wheat germ agglutinin and ovomucoid-gold complex). surface mucous cells and mucous neck cells secreted o-glycoproteins with n-acetylgalactosamine and n-acetylglucosamine residues at the non reducing terminus of the saccharidic chain. the secretory granules o ... | 1990 | 2081692 |
| characterization of galactose-containing glycoconjugates in the human retina: a lectin histochemical study. | seven specimens of morphologically normal formalin-fixed and paraffin-embedded human retina were studied using a panel of fourteen biotinylated lectins, all of which react with glycoconjugates containing galactose and n-acetylgalactosamine residues. agaricus bisporus (aba), bauhinia purpurea (bpa), phaseolus vulgaris (pha-e), peanut (pna) and ricinus communis (rca-i) agglutinins labeled photoreceptor cells prior to enzymatic predigestion. bpa and pna bound specifically to cones. the plexiform la ... | 1990 | 2091899 |
| use of peldri ii as a sublimation dehydrant in place of critical-point drying in fracture-label cytochemistry and in backscattered electron imaging fracture-label. | peldri ii, a fluorocarbon compound solid at room temperature, was used as a sublimation dehydrant in place of critical-point drying (cpd) in freeze-fracture cytochemistry. its applicability to the fracture-label replica method was demonstrated using freeze-fractured rat pancreas labeled with helix pomatia lectin-gold complex and phospholipase a2-gold complex for the detection of glycoconjugates and phospholipids, respectively. no difference in morphology and labeling pattern was detected in cpd- ... | 1990 | 2153786 |
| interaction of bluetongue virus with bovine lymphocytes. | freshly isolated, and established, cultures of bovine peripheral blood mononuclear leukocytes (pbmls) were exposed to bluetongue virus (btv) for the purpose of defining potential lymphotropism. pbml cultures were established in the presence of interleukin 2 (il-2) and mitogen and maintained either as bulk culture or were cloned prior to infectivity studies. all cultures appeared to be of the t cell phenotype based on the following characteristics: binding of t lymphocyte-specific lectins (i.e. p ... | 1990 | 2155289 |
| flow cytometric analysis of cell surface carbohydrates in metastatic human breast cancer. | helix pomatia agglutinin (hpa)- and concanavalin a (con a)-binding carbohydrate expression were studied on 32 tumour samples from primary adenocarcinoma of the breast and 12 samples from lymph node metastases. live cells were spilled from each of the fresh samples and the extent of fluorescent-labelled hpa and con a-binding was assessed by flow cytometry. the extent of brightness was expressed in a defined quantitative fashion and the percentage of positive cells was accurately determined from a ... | 1990 | 2167120 |
| characterization of the high mr glycoprotein (gp300) of equine herpesvirus type 1 as a novel glycoprotein with extensive o-linked carbohydrate. | the high mr glycoprotein (gp300) of equine herpesvirus type 1 was found to have an mr, estimated by sds-page, of over 400,000 and was confirmed as being a surface glycoprotein by 125i-labelling. in contrast to [3h]glucosamine, gp300 showed very low levels of [3h]glucosamine, gp300 showed very low levels of [3h]mannose incorporation. the mr of gp300 showed no detectable change upon treatment of purified virus with n-glycanase, and showed only a small change in virus-infected cells treated with tu ... | 1990 | 2172454 |
| time courses of lidocaine effects on sodium membrane currents in small and large neurons. | time courses of effects of lidocaine on sodium currents and sodium dependent action potentials were studied in somata of small and large neurons. cultured rat sensory spinal ganglion cells (diameter: 30 microns) and neurons of the buccal ganglion of helix pomatia (diameter: 150 microns) served as the test cells. the latency of the suppressive action of lidocaine was the longer the larger the of the cells was. maximal blocking effects occurred within 10 min in sensory spinal ganglion cells and wi ... | 1990 | 2177020 |
| effects of alcohol on lectin binding affinity in rat gastric mucosa. | fluoresceinated lectins were employed to qualitatively evaluate cell surface carbohydrates, with and without ethanol exposure, in rat stomach mucosae. rats received 1 ml of saline, or 50% or 100% ethanol orally. after 30 min, tissue samples of the glandular stomach were retrieved, cryosectioned, and incubated with one of a panel of lectins. another set of sections was preincubated with neuraminidase to remove sialic acid residues. qualitative evaluation of lectin binding showed that although sev ... | 1990 | 2194768 |
| transient expression of type 2 chain in a-active hexaglycosylceramide of rat small intestine at weaning time. demonstration by affinity chromatography and ceramide glycanase hydrolysis of a-active glycosphingolipids followed by gas chromatography and mass spectrometry of permethylated hexasaccharides. | the small intestine of 15- to 23-day-old rats was cut into four segments from the duodenum to the ileum. neutral glycosphingolipids were purified from each segment and submitted to thin-layer chromatography and immunostaining with the a005 monoclonal anti-a antibody. this antibody detected an hexaglycosylceramide located mainly in the duodenum during the postnatal development. in order to characterize hexaglycosylceramides, blood group a-active glycolipids were purified by affinity chromatograph ... | 1990 | 2221915 |
| helix pomatia agglutinin binds specifically to the golgi apparatus in cultured human fibroblasts and reveals two golgi apparatus-specific glycoproteins. | fluorochrome-coupled helix pomatia agglutinin (hpa), but not other lectin-conjugates with the same nominal specificity, bound specifically to the golgi apparatus in cultured human fibroblasts, revealing a cytoplasmic juxtanuclear reticular structure. unlike other golgi-binding lectins the hpa-conjugates did not bind to the cell surface membrane or pericellular matrix. experiments with 35s-methionine-labeled cells showed that hpa recognized two glycoproteins of mr 170,000 and 400,000 among the se ... | 1990 | 2228736 |
| [study of lymphocyte subpopulations using peanut, soybean and snail lectins]. | the method of penning in plastic dishes was to isolate the pna+ and pna- cell populations from the tonsil tissue. carbohydrate determinants of lymphoid cell surface membranes being at varying stages of antigen-independent and antigen-dependent differentiation have been studied with the use of peanut, soya and edible snail lectins conjugated with horse radish peroxidase. | 1990 | 2253858 |
| heterogeneity of the blood group abh antigens and variation in the expression of these antigens of secretory granules in human cervical glands. an electron microscopic observation using lectins and monoclonal antibodies. | cytochemical localization of blood group abh antigens was examined in secretory cells of human cervical glands by application of a post-embedding lectin-gold as well as immuno-gold labeling procedure using monoclonal antibodies. blood group specific lectins such as dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), griffonia simplicifolia agglutinin i-b4 (gsai-b4) and ulex europaeus agglutinin-i (uea-i) reacted with secretory granules but not with other cytoplasmic organellae su ... | 1990 | 2283312 |
| binding of the blood group-reactive lectins to human adult kidney specimens. | the binding of a panel of blood group-reactive lectins to frozen sections of human kidney was studied with a special emphasis on reactivity with endothelia and basement membranes. the blood group a-reactive lectins, all specific for alpha-d-n-acetylgalactosamine (galnac), helix aspersa (haa), helix pomatia (hpa), and griffonia simplicifolia i-a4 (gsa-i-a4) agglutinins bound to the endothelium in specimens with blood groups a and ab. in other samples, these lectins reacted predominantly with tubu ... | 1990 | 2297076 |
| ultrastructural comparative distribution of carbohydrates in human tracheal and frog palate mucosa using neuraminidase and lectin-colloidal gold complexes. | we have compared, at the ultrastructural level, the carbohydrate structure of glycoconjugates of the different types of secretory cells of the human tracheal mucosa (htm) and the frog palate mucosa (fpm), proposed as a model for studying bacterial adherence to mucus-coated respiratory epithelium. in addition to reactivity with concanavalin a and lens cullinaris agglutinin, reactivity of epon-embedded htm and fpm secretory granules was studied by transmission electron microscopy using neuraminida ... | 1990 | 2311103 |
| identification of glucose conjugates as major urinary metabolites of cannabidiol in the dog. | 1. three dogs were treated i.v. with cannabidiol (cbd) and urine collected at intervals to 30 h. 2. metabolites were extracted, converted into trimethylsilyl (tms) derivatives and examined by g.l.c.-mass spectrometry. 3. the major metabolites excreted at early times were identified as the phenol glucosides of 4"-hydroxy-cbd, 5"-hydroxy-cbd and 6-oxo-cbd. 4. these three oxidized metabolites were not found unconjugated, and none of the free oxidized metabolites in urine were found conjugated with ... | 1990 | 2333714 |
| ultracytochemistry of glycoconjugates in rat brunner's gland with labeled lectins. | mucous glycoproteins of rat brunner's gland were examined ultracytochemically to elucidate the intracellular localization and sites of glycosylation in relation to the functional polarity of the cell organellae using a combination of hydrophilic resin embedment and postembedding staining with labeled lectins. a few cis cisternae were stained with hpa (helix pomatia agglutinin, specific for terminal alpha-n-acetylgalactosamine) and dba (dolichos biflorus aggulutinin, specific for terminal alpha-n ... | 1990 | 2358769 |
| lectin cytochemistry reveals differences between hamster trachea and bronchus in the composition of epithelial surface glycoconjugates and in the response of secretory cells to neutrophil elastase. | hamsters exposed to an intratracheal instillation of human neutrophil elastase (hne) accumulate an abnormally high number of secretory granules in bronchial but not tracheal epithelial cells. we employed lectin cytochemistry to investigate possible differences in the epithelial cell surface glycoconjugate layer in trachea compared to bronchus which might explain the regional dissimilarity in response to hne. portions of glutaraldehyde-fixed trachea and bronchi were incubated in one of several fe ... | 1990 | 2363936 |
| surface array proteins of campylobacter fetus block lectin-mediated binding to type a lipopolysaccharide. | campylobacter fetus strains with type a lipopolysaccharide (lps) and a surface array protein layer (s+) have been found to be pathogenic in humans and animals. spontaneous laboratory mutants that lack surface array proteins (s-) are sensitive to the bactericidal activity of normal human serum. the ability of lectins to determine the presence of the s-layer and differentiate lps type was assessed. we screened 14 lectins and found 3 (wheat germ agglutinin, bandeiraea simplicifolia ii, and helix po ... | 1990 | 2387622 |
| characterization of candida antigens by crossed-immunoaffinoelectrophoresis. | the antigens of three candida albicans strains (3153 a, 3156 b and cbs 1905) and one c. tropicalis strain were studied by means of crossed-immunoaffinoelectrophoresis with the corresponding polyvalent antisera. most antigens (from 63.8% to 77.7% depending on the strain) were bound to concanavalin a-sepharose and about 20% to blue cibacron-sepharose for all the strains tested. free concanavalin a, wheat germ lectin-sepharose and helix pomatia lectin-sepharose revealed differences between c. albic ... | 1985 | 2409611 |
| alterations in glomerular lectin binding sites of human kidney as detected by fluorescence microscopy. | different lectins were used to study frozen sections of kidney samples showing alterations in routine immunofluorescence studies. arachis hypogaea agglutinin (peanut lectin, pna), lacking binding sites in normal glomeruli, bound to the glomeruli in two of the five samples studied, giving a granular fluorescence pattern. concomitantly with the appearance of pna-binding, binding sites for wheat germ agglutinin (wga) appeared to be lost at glomeruli. furthermore, changes in the expression of glomer ... | 1985 | 2415488 |
| lyophilization of protein-gold complexes. | conditions for dialysis, freezing and lyophilization of protein-gold complexes were established. lyophilized complexes formed by colloidal gold with staphylococcal protein a, with helix pomatia lectin and with horseradish peroxidase retained their original staining properties when reconstituted after several months of storage. | 1985 | 2416715 |
| tn epitopes, immunoreactive with ordinary anti-tn antibodies, on normal, desialylated human erythrocytes and on thomsen-friedenreich antigen isolated therefrom. | hybridoma generation, using specifically, maximally desialylated human blood group o erythrocytes (t rbc) as immunogen, and biochemical studies suggested the presence of immunogenic tn epitopes. galnac alpha-o, on t rbc. we therefore investigated by immunochemical means whether or not tn-specific epitopes immunoreactive with anti-tn antibodies present in ordinary human sera occur on t rbc and on thomsen-friedenreich (t) antigen prepared from them. we did detect the tn epitope with such antibodie ... | 1985 | 2417112 |
| regional heterogeneity of glycoconjugate distribution in the glomerulus revealed by lectin-gold cytochemistry and sds-page. | the authors have used sds-page and lectin overlay analysis in parallel with lectin-gold cytochemistry to identify helix pomatia lectin (hpl) binding glycoconjugates in rat kidney glomeruli. previous work revealed hpl binding sites only beneath podocyte foot process bases, where they contact the glomerular basement membrane. it is shown here that after neuraminidase digestion of thin sections of glomeruli before incubation with hpl-gold complexes, the number of hpl binding sites is markedly incre ... | 1986 | 2432793 |
| cytochemical characterization of mouse l1210 leukemia. | mouse lymphatic leukemia l1210 cells were characterized with polyclonal and monoclonal antibodies and lectins. the cells were found to have the phenotype igg-, thy-1.2-, lyt-1-, lyt-2-, asialo-gm1-, tl-, i-ad-, il-2r+, peanut agglutinin+, and helix pomatia lectin +/-. they retained expression of h-2kd and h-2dd. thus, these cells resemble "null" cells. | 1988 | 2466781 |
| tn epitope (n-acetyl-d-galactosamine alpha-o-serine/threonine) density in primary breast carcinoma: a functional predictor of aggressiveness. | this interpretive review attempts to dovetail advanced work by different groups of investigators on blood group and carcinoma (ca) glycoconjugates that have terminal, immunoreactive tn epitopes (galnac alpha-o-ser/thr), and on the interaction of those structures with complementary antibodies and lectins. fenlon et al. (1987) and leathem and brooks (1987) found a positive correlation between primary breast ca aggressiveness and its affinity for helix pomatia (hpa) lectin. this phenomenon was used ... | 1989 | 2467192 |
| lectin-binding sites in squamous cell carcinomas and keratoacanthomas. | the lectin-binding pattern of malignant and benign human epidermal tumours was studied. twelve squamous cell carcinomas and ten kerato-acanthomas were investigated, using fluorochrome-conjugated lectins. lectins used were canavalia ensiformis agglutinin (con-a), helix pomatia agglutinin (hpa), phaseolus vulgaris agglutinin (pha-l), and ricinus communis agglutinin (rca-120). two types of fluorescence pattern, intercellular 'pemphigus-like' and peritumoral, were noted. both allowed classification ... | 1988 | 2467485 |
| saccharide residues in human gingiva as revealed with fluorochrome-coupled lectins. | the histochemical binding of 16 fluorochrome-conjugated lectins to human marginal gingiva was investigated. of a total of 14 galactose/n-acetylgalactosamine (gal/galnac)-specific lectins, dolichos biflorus (dba), helix pomatia (hpa), and helix aspersa agglutinins (haa) were blood group a-reactive whereas griffonia simplicifolia i-b4 (gsa-i-b4) and sophora japonica (sja) agglutinins were blood group b-reactive. hpa, haa and gsa-i-b4 bound to all suprabasal epithelial cells and to vascular endothe ... | 1989 | 2542513 |
| rapid detection of herpes simplex virus with fluorescein-labeled helix pomatia lectin. | the use of fluorescein-conjugated helix pomatia lectin was shown to be as effective as fluorescein-conjugated monoclonal antibody reagents for the detection and differentiation of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) in mrc-5 cell culture. cells infected with hsv-1 generally displayed a pattern of nongranular or diffuse fluorescence, while cells infected with hsv-2 were identified by the production of fluorescent grains and flecks. this unique nonimmunological reagent, when u ... | 1989 | 2545739 |
| cytochemical demonstration of lectin binding-sites in the cuticle and tissues of acanthocheilonema viteae (filarioidea). | the lectin-gold technique was used for the ultrastructural localization of lectin binding sites on thin sections of lowicryl k4m embedded adult females, infective larvae and sds-2-mercaptoethanol-insoluble cuticle components of acanthocheilonema (dipetalonema) viteae. helix pomatia lectin (hpl) coupled to 14 nm gold particles, was used for the demonstration of n-acetyl-d-galactosamine-containing glycoconjugates. triticum vulgaris (wheat germ) agglutinin (wga) coupled to 10 nm gold particles afte ... | 1989 | 2566259 |
| isolation and identification of 6-desmethylnaproxen sulfate as a new metabolite of naproxen in human plasma. | a new metabolite of naproxen, 6-desmethylnaproxen sulfate (6-dmns), has been identified in plasma from normal and uremic subjects after oral administration of a single 500 mg dose of naproxen. tentative identification was achieved by the finding of an increase in the concentration of 6-dmn upon incubation of the plasma with arylsulfatase from helix pomatia or from aerobacter aerogenes. more definitive identification was established through demonstration that the hplc retention time of the conjug ... | 1989 | 2566468 |
| cytoplasmic intermediate filament proteins of invertebrates are closer to nuclear lamins than are vertebrate intermediate filament proteins; sequence characterization of two muscle proteins of a nematode. | the giant body muscle cells of the nematode ascaris lumbricoides show a complex three dimensional array of intermediate filaments (ifs). they contain two proteins, a (71 kd) and b (63 kd), which we now show are able to form homopolymeric filaments in vitro. the complete amino acid sequence of b and 80% of a have been determined. a and b are two homologous proteins with a 55% sequence identity over the rod and tail domains. sequence comparisons with the only other invertebrate if protein currentl ... | 1989 | 2583097 |
| a new method for hydrolyzing sulfate and glucuronyl conjugates of steroids. | a new method for hydrolyzing steroid conjugates (both sulfates and glucuronides conjugates) that is efficient, effective, and inexpensive is described. this method comprises incubation of the conjugates--after salting-out into ethyl acetate or elution from a c18 cartridge--with anhydrous methanolic hydrogen chloride (methanolysis) for 10 min. it has been successfully applied to our routine radioimmunoassay screening and gc/ms confirmation studies of steroids in prerace and postrace equine urine ... | 1989 | 2610346 |
| physicochemical properties of a human glycoprotein bearing blood group a activity. | a human erythrocyte glycoprotein was isolated and purified from blood of group a+1 by a procedure involving chloroform-methanol extraction and affinity chromatography on helix pomatia lectin-sepharose 6mb, and some of its physicochemical properties were determined. the resulting preparation was homogeneous as indicated by polyacrylamide gel electrophoresis. the glycoprotein contained nearly 60% carbohydrate and 40% protein. it was water-soluble and inhibited the agglutination of a-erythrocytes. ... | 1989 | 2613683 |
| reactions of the lectin anti-ahp from the edible snail helix pomatia with n-acetyl-d-galactosamine of streptococci. | the lectin anti-ahp obtained from the albumen gland of the edible snail, helix pomatia, was used for the routine identification of group c streptococci. without any exception all 1,045 strains of group c streptococci (s.equisimilis, and s.zooepidemicus, and s.dysgalactiae) were agglutinated by the helix lectin, but none of the 12,264 strains of group a, 1,346 strains of group g, 330 strains of group b as well as that of other streptococcal groups. the presence of type-specific t antigens of grou ... | 1989 | 2622116 |
| topographical and planar distribution of helix pomatia lectin-binding glycoconjugates in secretory granules and plasma membrane of pancreatic exocrine acinar cells of the rat: demonstration of membrane heterogeneity. | the lectin-gold technique was used to detect helix pomatia lectin (hpl) binding sites directly on thin sections of rat pancreas embedded in lowicryl k4m and on freeze-fractured preparations of rat pancreas submitted to fracture label. on thin sections of acinar cells, whereas the content of zymogen granules was negative or weakly labeled, the limiting membrane displayed a high degree of labeling. in the golgi complex, labeling by hpl was localized on the trans saccules and the limiting membrane ... | 1989 | 2672769 |
| experimental glomerulonephritis induced in rats by a lectin and its antibodies. | the aim of the present study was to investigate the events following interaction of antibody with an antigen planted on the surface of glomerular endothelial cell (gen). a lectin, helix pomatia agglutinin (hpa), was planted at the surface of rat gen by the perfusion of the isolated left kidney with neuraminidase (nrd) and hpa. a subsequent perfusion with igg fraction, but not with fab fragments, of rabbit anti-hpa serum induced formation of granular immune deposits (ids) on the surface of gen. w ... | 1989 | 2689748 |
| mitogenic lectins bind to the antigen receptor on human lymphocytes. | the specificity of interactions between mitogenic and non-mitogenic lectins and disulfide-linked cell surface receptors on human lymphocytes was explored. lysates (nonidet-p40) of surface-radioiodinated tonsil lymphocytes and t lymphoblastoid cells (hpb-all) were absorbed with lectin-agarose derivatives (or bovine serum albumin, bsa-agarose) or immunoprecipitated with appropriate monoclonal antibodies (mab). lectin eluates and solubilized immunoprecipitates were analyzed by two-dimensional (nonr ... | 1989 | 2703017 |
| lectin-gold cytochemistry of the golgi apparatus in rabbit luteal cells, with special emphasis on the formation of a lysosomal-type membrane. | in rabbit luteal cells embedded in glycolmethacrylate and stained with pta at low ph highly glycosylated membrane patches can be observed after vesiculation of the trans-golgi network. as these membranes could be prelysosomal, their sialic acid content was investigated by post-embedding labeling with limax flavus agglutinin (lfa)/fetuin-au. additional labeling of the golgi apparatus was performed with wheat germ agglutinin (wga)/ovomucoid au, ricinus communis agglutinini (rcai)/au and helix poma ... | 1989 | 2715051 |
| [experimental infection of 2 species of laboratory rodents with invasive larvae of elaphostrongylus cervi (nematoda, metastrongyloidea)]. | single doses (from 300 to 1000 larvi per an animal) of invasive larvae e. cervi cameron, 1931, obtained from experimentally infected snails helix pomatia l. were given to 17 guinea pigs and 17 golden hamsters. clinical nervous symptoms in the form of paresis and paralysis of limbs occurred only in the guinea pigs which were given a dose of 1000 larvi. these animals died in the period from the 75th to 117th day of infection. from their central nervous system single adult males and females of e. c ... | 1989 | 2728450 |
| demonstration of sugar residues in the ultimobranchial tubule and thyroid c-cells of the rat using peroxidase labelled lectins. | the distribution of various sugars, that contribute to the composition of the carbohydrate components of the glycoprotein in the ultimobranchial tubule and the thyroid c-cells has been studied, using eight peroxidase-labelled lectins (dolichos biblorus, glycine max, bauhinia purpurea, helix pomatia, arachis hipogaea, ulex europaeus i, limax flavus and sophora japonica). the results obtained seem to show that there are receptors for dolichos biflorus, glycine max, bauhinia purpurea, helix pomatia ... | 1989 | 2757235 |
| reactivity of visceral yolk sac endoderm with lectins: changes related to age and species. | the expression of receptors for lectins reacting with extraembryonal endoderm was compared between mouse and rat at different stages of gestation. fluorescein-conjugated helix pomatia agglutinin (hpa), dolichos biflorus agglutinin (dba), sophora japonica agglutinin (sja), peanut agglutinin (pna), bandeiraea simplicifolia agglutinin 1 (bsa-1) and gold-conjugated dba and pna were used. it was shown that the rat visceral endoderm does not express the receptors for hpa, while the mouse tissue does. ... | 1989 | 2762737 |
| synthesis and initial processing of gastric apomucin. | the initiation of the processing of apomucin was investigated using mucus glycoprotein synthesizing polysomes from rat gastric epithelial cells. the polysomes were isolated from cells labeled with [3h]palmitic acid and [14c]n-acetylgalactosamine, purified on helix pomatia-sepharose affinity column, dissociated to release peptidyl-trna, and chromatographed on deae-hplc column to separate peptidyl-trna complexes from the free and ribosomal rna and proteins. the analysis of the hplc purified peptid ... | 1989 | 2764978 |
| lectin histochemistry in ulcerative colitis and crohn's disease. | the glycoconjugate composition of intestinal goblet cell mucin was characterized according to the anatomical distribution of lectin-binding sites in surgically resected intestinal tissues and mucosal biopsy specimens obtained from 38 control subjects, and from 32 patients with the active phase of ulcerative colitis, and 12 with crohn's disease. immunoperoxidase labeling studies found that in control tissues binding by soybean agglutinin (sba), dolichos biflorus agglutinin (dba), wheatgerm agglut ... | 1989 | 2769448 |
| biosynthesis and secretion of enamel proteins in the rat incisor. | the synthesis and secretion of enamel proteins (eps) in rat incisors was examined using cytochemical and biochemical methods. radioautography after injection of 3h-methionine showed that ameloblasts in the presecretory, secretory, and maturation stages of amelogenesis actively synthesized and secreted proteins. immunocytochemistry with an antibody to mouse amelogenins revealed the presence of eps in the protein synthetic and secretory organelles of these cells at all three stages. labeling was a ... | 1989 | 2774207 |
| comparison of glycoconjugates at the surface of developing type ii pneumocytes and clara cells. | the apical surface coat of type ii pneumocytes and clara cells in pre- and post-natal rat lung was examined with lectin histochemical methods. lectins from helix pomatia (hpa), peanut (pna) and maclura pomifera (mpa) were conjugated with horseradish peroxidase and used to stain paraffin sections of fixed lung with or without certain pre-treatments. hpa and mpa were observed to react with almost all type ii pneumocytes at postnatal day 1. type ii pneumocytes that stained with a sialidase-pna sequ ... | 1989 | 2777637 |
| detection and isolation of antigen-specific b cells by the fluorescence activated cell sorter (facs). | a method is described for the isolation of antigen-specific b cells from immunized and subsequently boosted mice. antigen-specific b cells were stained by incubation with fluorescein isothiocyanate (fitc)-labelled antigen and then detected and isolated in a fluorescence activated cell sorter (facs). ovalbumin (ova) and helix pomatia haemocyanin (hph) were used as antigens in this procedure, yielding relative amounts of antigen-fitc-binding lymphocytes of 0.9 +/- 0.4% and 3.5 +/- 3.1%. the fitc-p ... | 1989 | 2784157 |
| comparative analysis of agglutinins from hemolymph and albumin gland of helix pomatia. | the hemolymph of helix pomatia contains a weak agglutinating activity. this lectin concentration was calculated to be about 1.8 micrograms.ml-1. among the different red blood cells tested, pronase-treated sheep erythrocytes were found to be the most suitable indicator cells. their agglutination could be inhibited by galnac and glcnac. the serum agglutinin was isolated by affinity chromatography using sephadex g-200 as the matrix. it exhibited a single band in discontinuous page. in the presence ... | 1989 | 2808854 |
| cytochemical and biochemical characterization of glycoproteins in forming and maturing enamel of the rat incisor. | biochemical and histochemical studies have shown the presence of various carbohydrates in enamel. using lectin-gold cytochemistry, we have examined the distribution of glycoconjugates containing n-acetyl-d-galactosamine (galnac) and/or n-acetyl-glucosamine (glcnac)/n-acetyl-neuraminic acid (neunac) residues in rat incisor ameloblasts and in forming and maturing enamel embedded in lowicryl k4m, lr gold, and lr white resins. the enamel proteins that contain these carbohydrate moieties were further ... | 1989 | 2809173 |
| the reaction of nitric oxide with copper proteins and the photodissociation of copper-no complexes. | the reactivity with nitric oxide was investigated for a number of type-1, type-2 and type-3 copper proteins azurin from pseudomonas aeruginosa (type-1 copper); bovine superoxide dismutase, diamine oxidase from pig kidney and galactose oxidase from dactylium dendroides (type-2 copper); haemocyanin from helix pomatia (type-3 copper); the blue oxidases ceruloplasmin from pig serum, and ascorbate oxidase from cucurbita pepo medullosa. type-1 copper formed complexes with no in the oxidised state, whi ... | 1987 | 2822126 |
| host cell-induced differences in o-glycosylation of herpes simplex virus gc-1. i. structures of nonsialylated hpa- and pna-binding carbohydrates. | lectins with narrow oligosaccharide specificities were established as probes to study the host cell influence on the biosynthesis of o-linked oligosaccharides of the herpes simplex virus type 1 (hsv-1)-specified glycoprotein c (gc-1). we found that only gc-1 and no other glycoprotein bound to the peanut lectin (pna), with main specificity for gal(beta 1-3)galnac. previously, we have shown that only gc-1 binds to the helix pomatia lectin (hpa), with main specificity for terminal galnac. the o-lin ... | 1987 | 2825412 |
| detection of b and t cells, with lectins or antibodies, in healthy and bovine leukemia virus-infected cattle. | lectins, polyclonal antibodies and monoclonal antibodies (mabs) were evaluated as markers for bovine lymphocytes obtained from healthy animals and from cattle infected with bovine leukemia virus (blv). in the blood from healthy cattle the proportion of cells identified as t lymphocytes with the lectin helix pomatia (hp) (67.8 +/- 6.2%) using the indirect immunofluorescence technique was similar to the proportion of cells identified by the mabs p5 (66.1 +/- 3.8%) and blt-1 (59.8 +/- 7.1%). the pr ... | 1988 | 2839934 |
| heterogeneity of apical glycoconjugates in kidney collecting ducts: further studies using simultaneous detection of lectin binding sites and immunocytochemical detection of key transport enzymes. | in the search for a functional role for the polarized glycoconjugates of rat collecting duct epithelial cells, the relation between binding of various lectins and expression of cellular transport enzyme profile of the cells was studied. for this purpose, principal and intercalated cells of rat kidney collecting duct were identified by morphological criteria and by their immunocytochemically determined content of (na+ + k+)-atpase and carbonic anhydrase (ca ii), respectively. various n-acetylgala ... | 1988 | 2853698 |
| isolation and identification of a new major metabolite of diflunisal in man. the sulfate conjugate. | a new metabolite of diflunisal has been identified in volunteers and patients after multiple dose administration. the metabolite was isolated from human urine by silica gel chromatography and was further purified by reversed phase hplc. arylsulfatase from helix pomatia and from aerobacter aerogenes completely hydrolyzed the isolated metabolite to diflunisal, although hydrolysis by bacterial arylsulfatase was extremely slow. electron impact mass spectra for diflunisal and its sulfate conjugate we ... | 1986 | 2868855 |
| hopantenic acid beta-glucoside as a new urinary metabolite of calcium hopantenate in dogs. | the metabolism of calcium hopantenate (hopa) was studied in beagle dogs. after oral administration of 14c-labeled hopa, 25.5% of the administered radioactivity was excreted in the urine within 24 hr, mostly in the form of unchanged drug. the only metabolite, accounting for 4.2% of the radioactivity in the urine, was isolated by hplc. the metabolite was hydrolyzed by the treatment of beta-glucuronidase (helix pomatia), acid phosphatase, or beta-glucosidase. these enzyme activities were not inhibi ... | 1986 | 2877836 |
| lectin-binding characteristics of wuchereria bancrofti microfilariae. | the binding of 10 different lectins to the surface of microfilariae of wuchereria bancrofti has been investigated. wheat germ agglutinin (wga) and helix pomatia lectin (hpa) bound specifically to the sheathed microfilariae indicating the presence of n-acetyl-d-glucosamine and n-acetyl-d-galactosamine respectively on the surface. exsheathed microfilariae did not react with any of the lectins. treatment of sheathed microfilariae with proteases resulted in increased binding of wga and hpa. such tre ... | 1987 | 2884837 |
| alterations in glycoconjugate expression following malignant transformation of human oropharyngeal squamous mucosa. | malignant transformation of the squamous mucosa in the human oropharynx may be accompanied by alterations in carbohydrate production. glycoconjugate expression was assessed by examining lectin-binding patterns and abh isoantigen expression in human biopsy specimens from nine cases of carcinoma in situ and ten cases of invasive squamous cell carcinoma as well as adjacent normal squamous mucosa. ten different fluorescein-conjugated lectins were used. each lectin binds to specific nonreducing end-t ... | 1989 | 2913429 |
| differential expression of galactose and n-acetylgalactosamine residues during fetal development and postnatal maturation of rat glomeruli as revealed with lectin conjugates. | a battery of fluorochrome- or peroxidase-coupled lectins, reacting with alpha- or beta-galactose (gal), terminal n-acetylgalactosamine (galnac), or gal-(beta 1-3)-galnac residues, was used to study the emergence and distribution of cellular glycoconjugates in developing and adult rat glomeruli. neuraminidase pretreatment of the specimens was applied to monitor the maturation of the glomerular sialoglycoprotein coat. in the adult glomeruli, the lectin conjugates applied reacted sparsely or not at ... | 1989 | 2923282 |
| biochemical and functional characteristics of the human leukocyte membrane antigen family lfa-1, mo-1 and p150,95. | the human leukocyte function-associated (lfa-1) antigen, the monocyte differentiation antigen mo-1 which is characterized as the c3bi receptor and the glycoprotein p150,95 are characterized biochemically. immunoprecipitations carried out with 6 different monoclonal antibodies (mab) against lfa-1 indicated that four mab (spv-l1, spv-l5, spv-l7 and spv-l11) were directed against the alpha chain, whereas mab clb54 and mhm-23 were found to react with the common beta chain of lfa-1, mo-1 and p150,95. ... | 1985 | 2933266 |
| protein blotting: detection of proteins with colloidal gold, and of glycoproteins and lectins with biotin-conjugated and enzyme probes. | methods to detect "native" proteins immobilized on nitrocellulose membranes in spot tests or on blots prepared from polyacrylamide slab gels after electrophoretic separation are described. gold sols were found to be useful as general stains for proteins: they are polychromatic, yield an indelible record, and are complementary to india ink as protein stains because these two stains have different sensitivities for a number of proteins tested. for detection of wheat germ lectin (wgl)-binding glyco ... | 1985 | 2984955 |
| different populations of herpes simplex virus glycoprotein c discriminated by the carbohydrate-binding characteristics of n-acetylgalactosamine specific lectins (soybean and helix pomatia). brief report. | from the herpes simplex virus specified glycoprotein c two fractions were isolated with affinity either for helix pomatia lectin (hpa) or soybean lectin (sba). the data indicated that hpa and sba, despite their mutual main specificity for n-acetylgalactosamine, recognize structurally different gc populations. | 1985 | 2994599 |
| b lymphoblastoid cell lines with normal and defective o-glycosylation established from an individual with blood group tn. | individuals with the tn blood group contain terminal serine/threonine-linked n-acetylgalactosamine residues in their blood cells. this is due to lack of udp-d-galactose: d-n-acetyl galactosamine beta-d-galactosyl transferase from part of their red cells and probably from their leukocytes. we have established b lymphoblastoid cell lines from such an individual by in vitro infection of his lymphocytes with epstein-barr virus. the original line contained a mixture of cells reactive and nonreactive ... | 1986 | 3006838 |
| characterization of a herpes simplex virus type 2-specified glycoprotein with affinity for n-acetylgalactosamine-specific lectins and its identification as g92k or gg. | extracts from herpes simplex virus type 2 (hsv-2)-infected cells were subjected to affinity chromatography with gel-bound helix pomatia lectin (hpa). only one hsv-2-specified glycoprotein was isolated by this procedure and the glycoprotein had an apparent molecular weight of 130 000 (130k). the hpa-binding glycoprotein was genetically mapped, using hsv-1 x hsv-2 intertypic recombinants into the short component of the hsv-2 genome. the mapping position, electrophoretic mobility and the antigenic ... | 1986 | 3007660 |
| in vitro attachment of mono- and oligosaccharides to surface glycoconjugates of intact cells. | we have synthesized glycosylhydrazines of various mono- and oligosaccharides and coupled these to periodate- or galactose oxidase-treated human red cells and k562 erythroleukemia cells. the optimal conditions for this carbohydrate modification of cells have been established. this method makes it possible to specifically elongate oligosaccharide chains of cell surface glycoconjugates with desired carbohydrates. in this way, new antigenic and receptor properties can be conferred to cells, and the ... | 1986 | 3013895 |
| lectin histochemistry applied to human nerves. | histologic sections of normal and pathologically altered human peripheral nerves were stained with a battery of 20 fluorescein isothiocyanate-labeled lectins to determine whether these histochemical reagents could be used to identify peripheral nerve injury. eight plant lectins--from canavalia ensiformis, lens culinaris, triticum vulgare (wheat germ), types e4 and l4 from phaseolus vulgaris, types i and ii from ricinus communis, and wistaria floribunda--were found to bind to normal and pathologi ... | 1986 | 3015069 |
| isolation of glycoproteins of parainfluenza i (sendai) by helix pomatia lectin column. | glycoproteins of sendai virus were successfully isolated on a column of helix pomatia lectin-sepharose 6mb following solubilization with nonidet p-40. this technique can be used as a preparative step for the study of viral glycoproteins. | 1986 | 3021254 |
| [cell surface receptors in lymphoid lines studied using lectins]. | the nature and distribution of some carbohydrate receptors on the cell membrane of transformed human b- and t-derived lymphocyte cell lines (rpmi-1788, daudi, jurkat, molt-4) are studied by ricinus communis agglutinin, soybean agglutinin, helix pomatia lectin, peanut agglutinin and wheat germ agglutinin conjugated with ferritin and colloidal gold. the quality of receptors and the manner of their distribution detected by different lectins are considerably different. | 1986 | 3023007 |
| t and b peripheral blood lymphocytes in normal and lymphocytotic sheep. | surface immunoglobulins (sig), peanut agglutinin (pna), spontaneous erythrocyte rosette (e-rosette) and helix pomatia (hp) marker were investigated in normal and bovine leukemia virus (blv)-infected sheep. in normal sheep, 19.3% +/- 4.9 of peripheral blood lymphocytes (pbl) were sig+, whereas 58% +/- 5.69 were pna+, and 19.6 +/- 5.2 were e-rosette forming cells (e-rfc). in blv-induced lymphocytotic sheep, sig+ cells in pbl reached 59.4% +/- 15.06. in the same animals, pna bound to 20.6% +/- 9.69 ... | 1987 | 3040327 |
| separation of human b lymphocytes on helix pomatia a haemagglutinin into two major fractions differing in responsiveness to t-dependent mitogen (pokeweed mitogen) or antigen (tetanus toxoid). | a b-cell fraction consisting of 70% of cells carrying the b-cell-associated b1 antigen, 15-20% of m1+ non-b cells, and less than 3% of t cells was prepared from the peripheral blood of healthy human donors, previously vaccinated with tetanus toxoid (tt). as assessed by immunofluorescence after treatment with neuraminidase, approximately 40-50% of the b cells had surface structures binding to helix pomatia a haemagglutinin (hp). the cells were separated into three fractions by affinity chromatogr ... | 1986 | 3081997 |
| comparative investigations of latin american trypanosomes with lectins and complement lysis test. | four-days old epimastigote culture forms of different stocks of trypanosoma cruzi from colombia, costa rica and mexico as well of colombian stocks of t. rangeli were tested with 27 lectins and the complement lysis test. while the stocks of t. cruzi and t. rangeli showed common agglutination reactions with the lectins of canavalia ensiformis and pisum sativum, only the stocks of t. cruzi were additionally agglutinated by ricinus communis-120, glycine maxima, helix pomatia, axinella polypoides, ba ... | 1986 | 3085200 |
| the sheep erythrocyte receptor and both alpha and beta chains of the human t-lymphocyte antigen receptor bind the mitogenic lectin (phytohaemagglutinin) from phaseolus vulgaris. | we have studied the interaction of mitogenic lectins such as phytohaemagglutinin (pha) and concanavalin a (con a) with both surface molecules which, by the use of monoclonal antibodies, are known to trigger t-cell mitogenesis. monoclonal antibodies recognizing the t-lymphocyte receptor for antigen (ti) and/or its associated structure, cd3, activate t cells. more recently, a second pathway of activation has been described which involves the sheep erythrocyte binding glycoprotein cd2, a surface mo ... | 1986 | 3085210 |
| the role of phosphate groups in the interaction of human c-reactive protein with galactan polysaccharides. | human c-reactive protein (crp) shows binding specificities for phosphate monoesters, polycations and for several other biological macromolecules lacking these ligands. we report here that the formerly observed interaction of crp with snail galactans, as exemplified by helix pomatia galactan, is not due to a lectin-like carbohydrate-binding reactivity, but, instead that crp obviously binds to phosphate groups that are minor constituents of these polysaccharides. structural analysis of the galacta ... | 1986 | 3086219 |
| lectin histochemistry of papillary and follicular carcinoma of the thyroid gland. | the lectin-binding properties of human follicular and papillary carcinoma were studied histochemically and compared with lectin binding to normal or goitrous thyroid tissue. well-differentiated minimally invasive follicular carcinoma showed a lectin-binding pattern essentially identical to those of the normal thyroid gland and benign adenomatous lesions. overtly invasive follicular carcinoma showed focal reactivity with some lectins that were nonreactive with normal follicular thyroid cells (sol ... | 1986 | 3089192 |
| receptors for erythrogenic toxin a of streptococcus pyogenes on human peripheral blood lymphocytes and other blood cells. | the binding of erythrogenic toxin a (eta) of group a streptococci on human peripheral blood cells was demonstrated light- and electronmicroscopically by labelling with eta-gold. double labelling experiments of lymphocytes and the use of purified t-lymphocytes revealed that the eta-receptor was confined to t cells. besides lymphocytes, monocytes and especially granulocytes and thrombocytes showed a significant eta-gold labelling. the lymphocyte eta-receptor was found to be not sensitive to trypsi ... | 1986 | 3090624 |
| adp-ribosyltransferase from helix pomatia. purification and characterization. | adp-ribosyltransferases from several higher eukaryotes have been purified and characterized, but little is known about adp-ribosyltransferases in lower eukaryotes. we have purified an adp-ribosyltransferase (ec 2.4.2.30) from helix pomatia. the enzyme has an apparent km of 26.7 microm. optimal conditions for the enzyme reaction are 17.5 degrees c and ph 8. the time course is linear during the first 10 min of the reaction. the enzyme is capable of poly-adp-ribosylation. the most highly purified p ... | 1987 | 3124818 |
| distribution of lectin-binding sites in oxyntic and chief cells of isolated rabbit gastric glands. | the distribution of lectin-binding sites in oxyntic and chief cells of isolated rabbit gastric glands was determined with seven fluoresceinated lectins, to ascertain which lectins might best be used in the biochemical characterization of cell membranes and glycoproteins of these two cell types. oxyntic cell canaliculi were labeled by wheat germ, helix pomatia, and peanut lectins, suggesting a predominance of n-acetylhexosamines. tubulovesicles were heavily stained by wheat germ, helix pomatia, a ... | 1988 | 3134267 |
| dolichos biflorus agglutinin (dba) reacts selectively with mast cells in human connective tissues. | dolichos biflorus agglutinin (dba) binds to n-acetyl-d-galactosamine (galnac) residues in glycoconjugates and agglutinates erythrocytes carrying blood group antigen a. in cryostat sections of various tissues from blood group-specified humans, fluorochrome-coupled dba bound preferentially to fusiform connective tissue cells and to certain epithelial cells. the connective tissue cells were identified as mast cells by their typical metachromasia in consecutive staining with toluidine blue. double l ... | 1988 | 3138306 |
| surface carbohydrate changes on onchocerca lienalis larvae as they develop from microfilariae to the infective third-stage in simulium ornatum. | use was made of seven fitc labelled lectins as tools to investigate the surface of onchocerca lienalis larvae as they develop through to the infective third-stage in a natural vector, simulium ornatum. the lectins were derived from canavalia ensiformis (con a), lens culinaris (lentil), triticum vulgaris (wheat germ), arachis hypogaea (peanut), helix pomatia, phaseolus vulgaris (kidney bean) and tetragonolobus purpureus (asparagus pea). between 70 and 100 living parasites were examined for each d ... | 1988 | 3142948 |
| structure, biosynthesis, and glycosylation of human small intestinal maltase-glucoamylase. | maltase-glucoamylase (mga) was immunoprecipitated from detergent extracts of brush border membranes of the human small intestinal mucosa. electrophoretic analysis of the precipitates under denaturing conditions revealed a single polypeptide of mr = 335,000 in the presence or absence of reducing agents. cross-linking of brush border membranes with the homobifunctional reagent dithiobis(succinimidylpropionate) did not result in considerable changes in the electrophoretic pattern of mga. in contras ... | 1988 | 3143729 |
| glycoconjugates of the human sperm surface: distribution and alterations that accompany capacitation in vitro. | we have studied changes in the binding of fluoresceinated lectins to human sperm during in vitro capacitation. we first determined the surface labeling pattern of viable sperm obtained by the swim-up procedure. sperm were labeled with 100 micrograms/ml fitc-conjugated lectin at 4 degrees c for 30 min. we simultaneously used hoechst stain 33258 as a supravital stain to help differentiate surface from intracellular lectin labeling. of 14 lectins studied, six (phytohemagglutinin-e, concanavalin a, ... | 1987 | 3147937 |
| alpha-n-acetylgalactosaminidase deficiency, a new lysosomal storage disorder. | a new lysosomal storage disease with autosomal recessive inheritance is described in two male siblings of 5 1/2 and 4 years of age. clinical manifestations started after 9 months of age with neurological symptoms, followed by progressive psychomotor deterioration. urinary oligosaccharide excretion was abnormal and showed a characteristic pattern on chromatography. enzyme assays showed a profound deficiency of lysosomal alpha-n-acetylgalactosaminidase in cultured fibroblasts, leukocytes and plasm ... | 1988 | 3149698 |
| gas chromatographic/mass spectrometric analysis of 19-nortestosterone urinary metabolites in man. | a sensitive and highly specific method based on capillary column gas chromatography/mass spectrometry has been developed for the detection of 19-nortestosterone (17 beta-hydroxy-4-estren-3-one) metabolites in urine. after intramuscular administration of 19-nortestosterone decanoate to man, urine samples were collected during several days and treated with helix pomatia digestive juice. the free steroids were extracted and converted into o-methyl-oxime-trimethylsilyl or the trimethylsilyl ether de ... | 1985 | 3160396 |
| structure of a ganglioside with cad blood group antigen activity. | the cad antigen is a rare erythrocyte blood group antigen expressed on both sialoglycoprotein and ganglioside structures. it is related both serologically and biochemically to the sda blood group antigen expressed on over 90% of caucasian erythrocytes. we reported previously that cad erythrocytes contain a novel ganglioside that binds helix pomatia lectin and inhibits human anti-sda antibody. we have now purified the cad ganglioside and determined its structure. the ganglioside contained glc-gal ... | 1988 | 3167001 |
| wheat germ agglutinin and helix pomatia agglutinin lectin binding on cochlear hair cells. | the fluorescein labelled lectins fitc-wga and fitc-hpa were used to identify specific carbohydrates in cochlear hair cells. wheat germ agglutinin (wga) bound with the cell coat of both inner and outer hair cells (ihc and ohc) suggesting the presence of either n-acetyl-d-glucosamine or sialic acid. in contrast, glycoconjugates with terminal n-acetyl-d-galactosamine residues that bind with helix pomatia agglutinin (hpa), were demonstrated inside the plasma membrane of outer hair cells. wga and hpa ... | 1988 | 3170357 |
| backscattered electron imaging of lectin binding sites in tissues following freeze-fracture cytochemistry. | recently, cytochemical techniques have been applied for localizing membrane components; however, transmission electron microscopy only provides two-dimensional information about their distribution. scanning electron microscopy, on the other hand, offers the possibility of examining the three-dimensional architecture of biological samples. the fracture-label cytochemical technique was combined with the backscattered electron imaging (bei) mode of the scanning electron microscope to visualize the ... | 1988 | 3199220 |