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diagnosis of wuchereria bancrofti filariasis by immunofluorescence using microfilariae as antigen.a new technique for the diagnosis of wuchereriasis is described. microfilariae treated with proteolytic enzyme were used as antigen for the immunofluorescent reaction. the specificity of the reaction is demonstrated in sera from persons who have a microfilariaemia, individuals with clinical symptoms of the disease but no microfilariaemia, those who have no clinical symptoms, but harbour other parasites, and in persons living in france, where the wuchereria bancrofti filariasis is absent.1976779683
[filarial eosinophilic lung diseases, value of immunology (apropos of 9 cases)].the study of nine cases of "eosinophilic lung" using specific immunological techniques indicates that the latter completes the geographical, clinical, radiological and hematological criteria of this syndrome. the search of circulating antibodies confirms the filarial etiology and individualises a remarkable immunological profile thus, suggesting a diagnosis in the case of occult filariases. the intensity of the immunological reactions brings a supplementary argument in favor of the hypothesis of ...1975779992
[parasitologic and serologic testing for bancroft's filariasis on the south-east coast of madagascar]. 1975779994
bancroftian filariasis in togo 1. a comparative field study of the membrane filtration concentration technique and conventional blood films.the membrane concentration filtration technique which has proved very successful in use in areas of subperiodic filariasis in the pacific, has been used to study an area of nocturnally periodic in togo. the results obtained were similar to those from areas of low endemicity in the pacific. the method was easy to apply under field conditions in africa and the taking of venous blood samples proved competely acceptable to a rural population in south-eastern togo.1976781957
parasitic infections in humans in west kalimantan (borneo), indonesia.a survey was carried out among inhabitants of eight villages in west kalimantan province (borneo), whereby blood smears were examined for malaria, stools examined for intestinal parasites and sera tested by the indirect hemagglutination test for antibodies to entamoeba histolytica and toxoplasma gondii. the prevalence of malaria among 3017 people examined was 5.6% (plasmodium vivax 2.8%, plasmodium falciparum 2.8%). brugia malayi microfilariae were found in 3.6% and wuchereria bancrofti in 0.3%. ...1976788263
[filariasis]. 1976788341
[bancroft filariasis in comores archipelago (immunologic study)].an immunological study has complemented the epidemiological survey on bancroft filariosis in the mayotte island (comores) carried out by brunhes et al., 1971. the search for antibodies to dipatolonema viteae and setaria labiotopapilosa was performed by means of electrophoresis and immunofluorescence in 87 individuals and 2 hydrocele fluids. immunoelectrophoresis confirmed the high endemicity ratio: 57 o/o of the individuals presented 1 to 7 precipitating lines; with immunofluorescence 55 o/o of ...1976791529
[chyluria in japan--report of the present status]. 1976792541
[displacentar transfer of fluorescent antibodies from mothers to their newborns in a wuchereria bancrofti endemic area in india (author's transl)].the immunofluorescent antibody test against d. viteae applied to mothers blood and the umbilical cord blood of their newborns showed a very close association of the two endpoint titers in 50 pairs of examinations. in 37 cases the titer of the child was corresponding with the titer of its mother, in 10 cases the newborn's titer was one dilution below, in 2 cases two dilutions below the mother's titer. the titers of newborns of 15 mothers with titers greater than or equal to 1:80 were in 11 cases ...1976795116
biochemical studies in human filariasis. 1976798723
a mass dissection technique for determining infectivity rate of filariasis vectors.a mass dissection method is described using an apparatus with a 55 mesh cotton sieve (244 micron pore size), which proved efficient in detecting the infectivity rate of bancroftian filariasis in culex p. fatigans. a recovery rate of more than 98% was obtained after one hour's settling when a known number of infective larvae was released through the sieve. in the comparative tests of individual- and mass-dissection of mosquitos from the same origin, the number of infective larvae recovered in the ...1979384057
factors that affect the intradermal test in diagnosis of filariasis in egypt. 1976801636
studies on bancroftian filariasis in liberia, west africa. ii. changes in microfilaraemia in a rural population some years after first examination.the population of eleven villages in the savanna area in upper lofa in the hinterland of liberia was investigated in 1973 and 1976/77 for infections with microfilariae of wucheria bancrofti. from a total of 1255 persons examined in 1973 16,3% were microfilaria carriers and in 1976/77 17,9% out of 1356 inhabitants were microfilaria positive. to obtain informations on the change of microfilaraemia in individuals, emphasis was laid in 1976/77 on the identification of those persons, who had been exa ...1979384627
microfilarial granuloma of the breast in a patient with tropical pulmonary eosinophilia.microfilariae found in a breast nodule of a patient with tropical pulmonary eosinophilia were identified as wuchereria bancrofti, confirming that the tropical pulmonary eosinophilia syndrome may be associated with infections caused by this species of filarial worm.1977869111
filariasis due to wuchereria bancrofti in haiti. 1977595106
histochemical differentiation of brugia, wuchereria, dirofilaria and breinlia microfilariae.histochemical demonstration of acid phosphatase activity in microfilariae gives sufficiently characteristic and consistent results for the differentiation of even closely related species. no difference could be detected among nocturnally periodic, nocturnally subperiodic and diurnally subperiodic brugia malayi, but they could readily be distinguished from b. pahangi. similarly, dirofilaria repens could be readily distinguished from d. immitis and b. booliati from b. sergenti. the enzyme distribu ...1978666387
a multidisciplinary study on bancroftian filariasis in jakarta.a study on bancroftian filariasis in jakarta has indicated that one person in one year could be exposed to 223,000 bites of culex pipens fatigans and to 1,941 infective-stage larvae of wuchereria bancrofti. blood surveys with 20 mm3 samples revealed a microfilaria rate of 6%. although some cases of hydrocele (4% of 272 males examined) were found, there was little evidence of severe filarial disease in either males or females. amongst wild-caught mosquitoes only 0.3% contained infective larvae, b ...1978366813
anopheles fluviatilis recorded as an experimental vector host of wuchereria bancrofti. 1976965711
[transmission of wuchereria bancrofti in the coastal sahelo-souadienne zone (little senegalese coast)]. 1978668035
a field trial of competitive displacement of aedes polynesiensis by aedes albopictus on a pacific atoll.prior laboratory studies and field observations suggested that it might be possible to reduce the size of the population of, or eliminate, aedes polynesiensis by the introduction of aedes albopictus. the former mosquito is the principal vector of nonperiodic filariasis caused by wuchereria bancrofti and the latter is a closely related species refractory to the development of human filariae. the practicability of such competitive displacement was studied by a field trial on a remote coral atoll w ...19761008133
filariasis in the minahassa.a survey concerning the occurrence of filariasis was carried out in likupang, a village in the minahassa, the norther tip of the island of celebes. we found four patients with elephantiasis of the leg. only of people living in their neighbourhood proved to be positive for microfilariae of brugia malayi. this result differs from surveys formerly carried out in whole sulawesi, where locally microfilariae of wuchereria bancrofti occurred next to those of b. malayi.19761014078
the exsheathment of brugia pahangi microfilariae under controlled conditions in vitro.two reproducible techniques for the exsheathment in vitro of microfilariae of brugia pahangi, and other sheathed microfilariae, are described. microfilariae were isolated from infected cat blood by filtration and suspended in hank's balanced salt solution. the first technique involved the incubation of isolated microfilariae for one hour in 20 mm cacl2 in a phosphate-free balanced salt solution, during which time approximately 90% of the microfilariae lost their sheaths. the second method of exs ...1979573989
metrifonate in onchocerciasis and lymphatic filariasis.metrifonate, in doses of 10 mg/kg for 3 consecutive days, demonstrated partial microfilaricidal activity against onchocerca volvulus in humans. higher doses were not investigated because of the toxic reactions obtained and the absence of monitoring of the blood cholinesterase levels. spaced doses of 10 mg/kg every 2 weeks or once monthly up to 3 doses showed neither micro- nor macrofilaricidal activity against wuchereria bancrofti.1978675839
[evolution of filarial hemagglutinating antibodies 1 year after a single dose of diethylcarbamazine].the authors compare the serological results obtained before and after a single dose with diethylcarbamazine (6 mg./kg.) in a homogeneous and strongly infected with wuchereria bancrofti var, pacifica population. they point out a statistically significant decrease of the hemagglutining antibody titres of filarian patients.19761037437
[filarian elephantiasis in french polynesia (wuchereria bancrofti var. pacifica). ii. biological aspects].realized in french polynesia among 274 patients with elephantiasis, this survey studied the microfilaremia, the eosinophily, the immunoglobulin titers and the antifilarian antibodies (done by passive hemagglutination) for wuchereria bancrofti var. pacifica. patients with elephantiasis seldom have circulating microfilariae in their blood. hypereosinophily is frequent but rarely high. it is similar to the one patients with microfilaremia. there is a trend towards neutropenia during lymphangitic cr ...1978385164
a new technique for the determination of microfilarial densities in onchocerciasis.precise measurement of the parasite load in helminthic infections makes it possible to correlate morbidity, the individual response to treatment, and the best control methods. a membrane filtration technique was recently developed for quantifying wuchereria bancrofti microfilaraemia. the present paper describes its application to onchocerciasis in a study on 107 patients in northern togo. the technique is cheap and simple to apply under field conditions, and is extremely efficient at recovering ...19761085663
filarial arthritis in the sepik district of papua new guinea. 19751092984
[letter: activity of l-tetramisole in lymphatic filariosis due to wuchereria bancrofti. preliminary results]. 19751094417
[ecology of culex pipiens fatigans larvae in an area of high endemicity of bancroftian filariasis (author's transl)].culex pipiens fatigans in the main vector of bancroftian filariasis in the mayotte island (comores) where it imposes an important health problem. the breeding-sites of c. p. fatigans are either man-made (latrines, cesspools, various containers), or natural (polluted water of estuaries of some rivers). the man-made breeding-sites are not similarly distributed in the different localities of the island. their distribution varies according to the customs of the inhabitants, size and site of the comm ...19751096384
algorithms inthe diagnosis and management of exotic diseases. vi. the filariases. 19751099150
[on the migration of the third stage larvae of wuchereria bancrofti in anopheles gambiae (author's tranls)].the migratory pattern of the third stage larvae of wuchereria bancrofti from the thorax into the head of anopheles gambiae has been studied. a total of 1269 infected mosquitoes were dissected and examined from the 7th through the 19th day after the blood meal. the filarial worms migrated from the thorax through the abdomen before they reached the head of the mosquito. third stage larvae first appeared in the abdomen on the 9th day after infection, where their number increased progressively up to ...19751103391
studies of the susceptibility of culex pipiens fatigans from non-endemic filarial areas to urban wuchereria bancrofti. 1979386965
[effects of a unique dose of mebendazole on microfilaremia in 50 carriers of microfilaria of wuchereria bancrofti, pacifica variety].treatment by mebendazol were given to 50 patients divided in 3 groups, the first on having s single dose of 200 mg., the second one a single dose of 300 mg. and the last one a single dose of 400 mg. there was practically no unpleasant side effect. we noticed a delayed but notable effect on microfilaremia sensibly lower than diethylcarbamazine action in similar conditions. it would be interesting to try heavier doses or iterative doses.19751104212
filarial mesenteric lymphadenitis with ileal perforation. 19751104715
bancroftian filariasis. 19761107961
wuchereria bancrofti microfilarial antigen in the diagnosis of human filariasis by skin test. 1979387950
acid phosphatase activity in wuchereria bancrofti microfilaria. 19761255378
bancroftian filariasis in egypt. 1979390796
microfilaria of wuchereria bancrofti in bone marrow smear.microfilariae of wuchereria bancrofti were detected in seven cases of anemia in persons with asymptomatic filariasis. in three cases microfilariae could not be detected in the blood. their presence in bone marrow may indicate the ability of microfilariae to cross the vessel wall.19761259081
bancroftian filariasis: a comparison of microfilariae counting techniques using counting chamber, standard slide and membrane (nuclepore) filtration.three techniques were compared for counting microfilariae of wuchereria bancrofti in blood specimens--a standard slide method, the counting chamber technique (cct) and a membrane filtration technique (mfct). the cct was much more sensitive than the slide method, the difference in sensitivity between these two methods probably being due mainly to the loss of microfilariae during the dehaemoglobinizing process prior to staining in the standard slide method. there was good correlation between the t ...1979393190
specific and sensitive igg4 immunodiagnosis of onchocerciasis with a recombinant 33 kd onchocerca volvulus protein (ov33).the full length cdna of the immunodominant ov33 protein of onchocerca volvulus was expressed in e. coli using various vector constructs. expression was best with the vectors pgex2t and pcg808fx, yielding fusion protein ov33-gst and ov33-mbp, respectively. purified fusion protein ov33-gst and o. volvulus antigen extracts (ovag) were used to compare antibody responses (igm and igg-subclasses) of patients infected with o. volvulus, brugia malayi, wuchereria bancrofti, mansonella perstans/loa loa an ...19921281926
[comparative study of technics of detection of wuchereria bancrofti lymphatic filariasis]. 1977589737
preliminary screening of antifilarial activity of levamisole and amodiaquine on wuchereria bancrofti.a single dose of levamisole of 3 mg/kg had a marked microfilaricidal effect in some persons harbouring wuchereria bancrofti, but there was considerable variation in the response. in some cases blood examinations for microfilariae remained negative or at a low level for several months. persons with high pretreatment microfilaraemias responded less to therapy than did those with low counts. the main side-reaction to levamisole was fever, which began six to eight hours after treatment. combined dec ...1979393191
identification and characterization of a parasite antigen in the circulating immune complexes of onchocerca volvulus infected patients.a peg-elisa was used to demonstrate parasite specific immune complexes in a significant proportion (25/26) of onchocerca volvulus infection sera from sierra leone. the parasite antigen was detected using a peroxidase-conjugated rabbit serum raised to the bovine parasite o. gibsoni. controls including european control serum, endemic control serum and rh+ sera gave consistently low readings. characterization of the parasite component in the immune complexes by western blotting demonstrated a heat ...19921284095
observations on culex quinquefasciatus say in relation to transmission of filariasis due to subperiodic wuchereria bancrofti in samoa.observations on culex quinquefasciatus say in samoa during a study of the epidemiology and control of subperiodic bancroftian filariasis are reported. the man-biting rate of c. quinquefasciatus was comparable with that of aedes (finlaya) samoanus gruenberg in one indicator village and lower in another. the house-frequenting behaviour of the two species was similar. culex quinquefasciatus was active throughout the night with peak biting during midnight hours, and its survivorship, estimated by tw ...19921288433
microfilaraemia survey in vientiane, laos.during the period june 1977-january 1978 a microfilariaemia survey was conducted for the first time in 27 villages (population: 9,300) of vientiane province of laos. a total of 2,339 persons, 1,372 males, 967 females, mostly adults, were blood tested between 2000--2400 hours; one thick smear, approximately 20 c.mm, was collected from each one and stained with 10% giemsa for 30 minutes; only one positive was detected with microfilaria of w. bancrofti in a 22-year-old male; unusually the sheath wa ...1979395663
a comparative study of acetylcholinesterase activity in bovine (s. cervi) and human (b. malayi, w. bancrofti) filaria.setaria cervi, a bovine filarial parasite, contains a significant amount of acetylcholinesterase (ache) activity with microfilaria having five to ten times more ache activity than female and male adult worms, respectively. because ache shows substrate specificity and hydrolyzes acetylthiocholine but not butrylthiocholine, this parasitic enzyme is likely a true acetylcholinesterase. the latter also resembles an ache enzyme in the human filarial parasite b. malayi which hydrolyzes acetylthiocholin ...19921293431
studies on human filariasis in malaysia: immunodiagnosis using indirect immunofluorescence.the indirect immunofluorescence test using sonicated microfilariae of brugia malayi has been evaluated on 173 sera from patients and persons exposed to wuchereria bancrofti and b. malayi in endemic areas of peninsular malaysia. in the microfilaria-negative group, without signs and symptoms of filariasis 55/62 sera (89%) had titers of 1:16 and less. in the microfilaremic groups and in the amicrofilaremic cases with clinical filariasis, all the sera tested were positive, with the antibody titers r ...1979395664
[efficacy of ivermectin for control of microfilaremia recurring after treatment with diethylcarbamazine. i. clinical and parasitological observations].we compared the efficacy of a single dose of ivermectin with that of a standard course of deithylcarbamazine (dec) for the control of microfilaremia in 60 patients with banrroftian filariasis who had developed recurrent microfilaremia after each of three or more prior treatments with dec. the study was done as a randomized, double-blind trial. complete, but transient clearance of microfilaremia was observed in both treatment groups. one year later, recurrent microfilaremia was present in 7 patie ...19921307270
bancroftian filariasis and other parasitic infections in the middle fly river region of western papua new guinea i. clinical, parasitooogical and serological studies. 1979395913
tanzania filariasis project: a provocative day test with diethylcarbamazine for the detection of microfilariae of nocturnally periodic wuchereria bancrofti in the blood.in coastal tanzania, an area where the microfilariae (mf) of wuchereria bancrofti exhibit nocturnal periodicity, the administration of 2 mg diethylcarbamazine (dec) per kg body weight in the daytime provoked mf to enter the peripheral blood. in persons on normal daily activities the daytime dec provocative method proved to be as sensitive in detecting microfilaraemia as was the examination of night blood. its use in routine surveys is therefore justified. although mf densities by day and night w ...1979396052
detection of circulating filarial antigen in bancroftian filariasis. 1979397922
[follow-up survey on the long-term effects of dec medicated salt in the control of filariasis bancrofti (author's transl)]. 1979400290
seasonality of adult culex quinquefasciatus and transmission of bancroftian filariasis in pondicherry, south india.seasonal variations in biting culex quinquefasciatus and transmission of bancroftian filariasis were investigated in pondicherry, south india. the biting density of c. quinquefasciatus, the principal vector species, was lowest in the summer months and higher during the monsoon and winter months. the survival of the vectors as indicated by the proportion of parous mosquitoes was found to be less in the summer season. biting mosquitoes with infective stage larvae were not encountered during the ho ...19921356298
antigenic mimicry between piperazine derivatives and wuchereria bancrofti microfilariae. 19921356307
circadian rhythms in the mammalian central nervous system, blood pressure and parasites--hobbies of rudolf engel. 1979400292
the natural decline of wuchereria bancrofti infection in a vector control situation in the solomon islands.in a situation where filariasis and malaria are transmitted by the same vector, as seen here in the solomon islands, the malaria eradication programme aimed at controlling the vector, was found to have an effect on both diseases. in an area of choiseul island first surveyed by the author in 1970, three follow-up surveys were conducted--in 1974, 1975 and 1976. these showed a progressive decrease in persons infected. when the densities, especially the median microfilarial counts, were expressed as ...1977595094
[trial of the combination of diethylcarbamazine (notezine)-levamisole (solaskil) in the control of filariasis by wuchereria bancrofti var. pacifica in french polynesia]. 1979400401
studies on quantitative aspects of the transmission of wuchereria bancrofti.feeding experiments with anopheles gambiae and culex fatigans infected with wuchereria bancrofti showed that the mosquito females were losing about 20% of their worm burden when they were feeding on a blood chamber as well as on the guinea pig. 6.6% of all mature larvae found in the mosquitoes succeeded in entering the final host. a striking high infection rate was observed in those mosquitoes which refused to feed on a guinea pig. these mosquitoes have also to be considered when the loss of fil ...1976941249
experimental wuchereria bancrofti infection of culex quinquefasciatus and aedes aegypti.laboratory-derived females of culex quinquefasciatus and aedes aegypti were experimentally infected with wuchereria bancrofti by allowing the insects to feed on infected volunteers of known microfilariae density between 24.00 and 2.00 hours. of the 240 mosquitoes used for the experiments only 67 (55.8%) of c. quinquefasciatus and 83 (69.2%) of the ae.aegypti actually fed on the volunteers' blood. after 16 days post-infection period, 50 (74.62%) of the engorged c. quinquefasciatus harboured infec ...19921416219
[ivermectin or diethylcarbamazine in spaced dosages in bancroft's filariasis: which protocol?].58 apparently healthy polynesian wuchereria bancrofti carriers were randomly allocated to 5 treatment groups: 1) two annual doses of ivermectin 100 mcg/kg, 2) three semi-annual doses of ivermectin 100 mcg/kg, 3) two annual doses of diethylcarbamazine (dec) 3 mg/kg, 4) semi-annual doses of dec 3 mg/kg, 5) two annual doses of dec 6 mg/kg. results observed during the 12-month period which followed last treatment have confirmed that efficacy of ivermectin is higher than that of dec in terms of immed ...19921417159
subcutaneous nodules as a manifestation of onchocerciasis. a rare condition in the united states.although infection with tissue nematodes is a significant health problem in many parts of the world, such cases are unusual in the continental united states. this report describes a case of onchocerca volvulus infection, acquired by a 38-year-old female missionary in northern brazil, that manifested as subcutaneous nodules in the groin and flank two years after the patient's return to the united states. a year previously, a wrong diagnosis of wuchereria bancrofti was made at biopsy. at that time ...1976942304
a survey for wuchereria bancrofti in ethiopia. 1976976247
antigenicity of a protective recombinant filarial protein in human bancroftian filariasis.a 92-kda fusion protein that encodes amino acids 1-479 of a 62-kda brugia malayi antigen induces resistance to microfilariae in mice. the antigenicity of this recombinant protein was explored in asymptomatic residents of wuchereria bancrofti-endemic areas of papua new guinea and egypt. unlike sera from individuals in nonendemic areas, sera from residents of endemic areas contained igg3 antibodies (up to dilution 1:1280) reactive with the fusion protein. there was little or no recombinant antigen ...19921431265
study of human and parasitic factors in relation to bancroftian filariasis in egypt.the human leucocytic antigen (hla) and the histochemical reaction of microfilaria were studied by using cytotoxicity test and microfilarial acid phosphatase activity respectively to evaluate the role of the genetic factors and the parasitic factor in the pathogenesis of filariasis. statistical significant association was found between hla-b7 and the susceptibility for retaining microfilariae in blood and the development of clinical manifestations of filariasis. histochemical study reported more ...19921431291
comparative efficacy of the stained blood-film and counting-chamber technique for the diagnosis of wuchereria bancrofti. 19761032929
[activity of levamisole (solaskil) in lymphatic filariasis caused by wucheria bancrofti (variety pacifica].a 6 mg./kg. daily dose of levamisole was given during three days to ten carriers of microfilarias wuchereria bancrofti in tahiti. no action against adult worms was noticed. the immediate microfilaricid action was at least equivalent to the action of dec. however the decrease of microfilaremia was not so long with levamisole than with dec given to a lower dose. lastly the amount of reactions after a treatment by levamisole is notable.19761037387
[eosinophilia and lymphatic filariasis in french polynesia].an important hypereosinophilia is usually regarded as an interesting argument of presumption in lymphatic filariasis. this present report concerns 278 polynesians people infected with wuchereria bancrofti pacifica. six kinds of patients were analysed: carriers of microfilarias, patients with elephantiasis, with lymphangitis attacks, new infections, carriers of microfilariasis recently treated with diethylcarbamazine and, at least, 100 fellows without filariasis. the results which were observed d ...19761037617
on the developmental velocity of wucheria bancrofti larvae in vector mosquitoes of different susceptibility to filarial infections.the developmental velocity of wucheria bancrofti larvae was investigated in mosquito strains with different susceptibility to this filarial species. high susceptibility of the vector strains favoured fast filarial development, e.g. between 17.1 and 25.9% of all discovered larvae on day 13 p.i. had already migrated to the head and mouth parts of the more and partly even highly susceptible anopheles gambiae, culex pipiens quinquefasciatus and aedes aegypti, respectively, whereas between 0 and 3.4% ...19921456467
hyperendemic subperiodic bancroftian filariasis: a search for clinical and immunological correlates of microfilaraemia.a study was carried out in the kingdom of tonga, an area of hyperendemic bancroftian filariasis, to determine whether correlations could be made between microfilaraemia, as diagnosed by membrane filter concentration, and immunological (skin test, immunoglobulin levels) or clinical findings. there was no relationship between the presence or degree of microfilaraemia and any clinical manifestation or skin test reaction. the skin test positivity rate for microfilaraemic and amicrofilaraemic individ ...1976800355
field trial on control of bancroftian filariasis using common salt medicated with diethylcarbamazine. 1976828099
wuchereria bancrofti: the staining of the microfilarial sheath in giemsa and haematoxylin for diagnosis.indigenous cases of bancroftian filariasis have recently been identified in the country for the first time. the diagnosis of such infections depends on demonstrating the characteristic sheathed microfilariae of wuchereria bancrofti in the blood. giemsa, a stain recommended by many authors, was found to be unsuitable as the microfilarial sheath, an important distinguishing feature, does not take up the stain. in contrast, the sheath stains clearly in haematoxylin. the differences in the staining ...1977919785
a case of bancroftian filariasis. 19751104003
studies on filariasis in the pacific. 4. the application of the membrane filter concentration technique to a survey of wuchereria bancrofti filariasis in kepu district, jakarta, indonesia.a blood survey for filariasis was carried out in the kepu district of jakarta. examination of stained 20 c.mm finger-prick blood thick films and membrane filter concentration (mfc) of 1 ml venous blood. the youngest age group examined, 5-9 years old revealed a mf rate of 17% of which 71% were of a low density character detected by mfc only. the mf rate appears to remain relatively constant from the 10-14 year old group onward, approximately 35%, but at all age groups at least half the infections ...19751166343
circadian periodicity of wuchereria bancrofti in liberia and its effect on the results of a prevalence study.the harmonic wave curve of sasa and tanaka (1972), describing microfilarial periodicity, has been fitted to observations on 38 persons infected with w. bancrofti in liberia, as well as to data previously obtained from the same country by other authors. the periodicity of the liberian strain was similar to that of periodic forms of w. bancrofti in southeast asia and japan. the effect of periodicity on microfilaraemia prevalence survey results has been examined, using data from a recent study in c ...19761258142
community participation in the control of filariasis.a programme was launched in 1986 for the control of brugian filariasis in an area of high endemicity in southern india. sustained community involvement was achieved by integrating economic measures to improve the lot of the people with the creation of widespread awareness about the cause of the disease and about the possibilities of combating it.19921418331
pen-elisa for immunodiagnosis and immunomonitoring of bancroftian filariasis. 19921428992
natural infections of dirofilaria immitis in aedes (stegomyia) polynesiensis and aedes (finlaya) samoanus and their implication in human health in samoa.dirofilaria immitis infections were observed in aedes polynesiensis and ae. samoanus in samoa, together with wuchereria bancrofti infections, in a study on sub-periodic bancroftian filariasis during 1978-1980. in the 4 indicator villages, the infection rate in ae. polynesiensis was 0.46% and the infective rate 0.09% (15,223 mosquitoes were dissected). the infection rate in ae. samoanus was 0.20% and the infective rate 0.08% (10,089 dissected). in 45 selected villages throughout the country, ae. ...19921440786
w. bancrofti as a causal agent of polymyositis.a case of polymyositis associated with bancroftian filariasis in an adult male who presented with generalised painful swelling and weakness of muscles is presented. he had elevated muscle enzymes, a myopathic emg pattern, focal vasculitis on gastrocnemius muscle biopsy and w. bancrofti in the peripheral blood. there was clinical, biochemical and histopathological evidence of resolution of the disorder and total clearance of microfilaremia with diethyl carbamazine (dec) therapy.19921484032
a cloned antigen for serological diagnosis of wuchereria bancrofti microfilaremia with daytime blood samples.by differentially screening an adult brugia malayi cdna library with sera from microfilaremic and amicrofilaremic donors infected with wuchereria bancrofti, we have identified a novel parasite antigen denoted sxp-1. recombinant sxp-1 filarial antigen is preferentially recognized by sera from microfilaremic persons with bancroftian filariasis and from skin snip-positive patients with onchocerciasis. antibodies to sxp-1 are restricted to the igg4 subclass and gradually decline after treatment with ...19921484551
wuchereria bancrofti infection in human and mosquito populations of a polynesian village ten years after interruption of mass chemoprophylaxis with diethylcarbamazine.in 1991, a study on wuchereria bancrofti microfilariae (mf) and infection rates was carried out in the human and mosquito populations of a polynesian village where, 10 years before, the mf prevalence rate was 6.4% and twice-yearly mass treatment with 3 mg/kg of diethylcarbamazine (dec) was interrupted. venous blood samples were collected from 575 (97%) individuals aged 15 years or more, of whom 122 (21.4%) were mf positive. the mf carrier prevalence rate was 27.4% in males, significantly higher ...19921440820
[alfred th. leber (1881-1954): a pioneer in tropical ophthalmology. missing in the south seas--rediscovered in india].in spite of the brief duration of german colonial rule during that period tropical medicine enjoyed a remarkable growth and development. this is the first account of the career of the pioneer of tropical ophthalmology, alfred theodor leber (1881-1954); medical history had previously reported him missing in java after the 1st world war. his career was greatly influenced by his uncle, theodor leber (1840-1917), the founder of experimental ophthalmology. alfred leber was the one who combined teachi ...19921453664
wuchereria bancrofti induced pancreatitis.a case of pancreatitis of filarial origin is reported for the first time. the patient presented with chronic pancreatic pain. laparotomy revealed an inflammed pancreas and fine needle aspiration cytology confirmed pancreatitis. imprint of a peripancreatic lymphnode revealed a microfilaria. he was successfully treated with diethyl carbamazine.19921488798
some observations on filariasis in western samoa after mass administration of diethylcarbamazine.an extremely efficient diethylcarbamazine administration campaign to eradicate wuchereria bancrofti has been carried out in western samoa. the use of the membrane-filtration technique has shown that a large number of people exhibit extremely low microfilarial densities, often with less than 10 in 1 ml of venous blood. it was found that one of these low level microfilaria carriers readily infected the local vector aedes polynesiensis and that development took place to the infective stage. it was ...19761265818
critical analysis of an epidemetrons model for the assessment of bancroftian filariasis endemicity in some areas in egypt.this study attempted a trial fit of observed epidemiological data in egypt using the sasa-who-southgate epidemetrons model to quantify the endemicity and risk of transmission of filariasis. the geometric and arithmetic means (gm, am) were tried in addition to the recommended four epidemetrons of the model, prevalence rate (pr); median microfilaria density (mfd50); and linear regression coefficients (a and b). data were based on microfilaria counts in 20 mm3 finger-prick night blood samples from ...19921495122
melanization response of two mosquito species against wuchereria bancrofti.the melanization response of two mosquito against wuchereria bancrofti was studied under different conditions of temperatures, adult age and feeding regimes. the two mosquito species were culex pipiens, a susceptible species for w. bancrofti transmission and aedes caspius, a refractory one.19921500787
rapid diagnosis of bancroftian filariasis by acridine orange staining of centrifuged parasites.a rapid diagnostic test for detection of microfilaremia using a microhematocrit tube precoated with acridine orange (the quantitative buffy coat [qbc] tube) was compared with a conventional 50 microliters-thick blood film (tf) in 119 volunteers in an area of recife, brazil that was endemic for wuchereria bancrofti. both the qbc and the tf techniques were 100% specific, and thus positive predictive values were equal at 100% for each technique in all subjects studied. both techniques had equal neg ...19921281967
characterization of a muscle-associated antigen from wuchereria bancrofti.a recombinant clone, wbn1, isolated from a genomic expression library of wuchereria bancrofti and showing restricted specificity at the dna level (southern and pcr analyses) for wuchereria bancrofti and brugia malayi has been previously described. sequence analysis of wbn1 indicated that it had notable similarity to myosin. further characterization using in situ hybridization has localized the mrna in the muscle of the adult parasite and in the microfilariae. rabbit polyclonal antiserum, raised ...19921283597
in vitro and in vivo effect of diethylcarbamazine on the activity of acetylcholinesterase from wuchereria bancrofti infected human serum.the acetylcholinesterase (ache) activity was measured in human serum from persons infected with the filarial parasite wuchereria bancrofti. the asymptomatic microfilaremic serum showed five times increase in ache-activity as compared with normal serum, whereas only little difference was observed in serum from patients with elephantiasis. similar results were obtained when the enzyme activity was measured in the immune complexes precipitated with polyethyleneglycol. further, the effect of the ant ...19921519032
bancroftian lymphangitis in northeastern brazil: a histopathological study of 17 cases.the histological features in 17 patients (3 males and 14 females) with bancroftian lymphangitis, apparently not associated with lymphadenitis, are described. degenerative alteration in the worms and a severe inflammatory process were observed in the great majority of the cases. the patterns of tissue reaction varied, including exudative, infiltrative and granulomatous lesions. eosinophils were present, usually in great numbers. thirteen out of 14 female subjects showed involvement of the breast ...19921560478
mass treatment of filariasis using dec-medicated salt.diethylcarbamazine (dec)-medicated salt was used to control bancroftian filariasis in 20 endemic counties and cities of fujian province in china. the population was 5,189,126 and the microfilaria (mf) rates were between 1.56 and 11.81%. the total dose of dec was 6-10.5 g per person over a period of 2-4 months. six to 9 months after treatment the mf rate was reduced to 0-0.57% with a mean of 0.07% (621/823,796). in 184 villages sampled one year post treatment the mf rate was 0.22% (371/171,067) a ...19921560482
adult filarial worm (wuchereria bancrofti) in a benign ovarian tumour.a case of an adult filarial worm (wuchereria bancrofti) associated with a benign ovarian tumour and a cyst is presented. possible reasons for the findings of a well preserved part of the worm and a foreign body granuloma in the lymphatic subendothelium are discussed.19921291144
evaluation of two different antigens in immunodiagnosis of bancroftian filariasis using elisa and ihat.crude w. bancrofti microfilarial and l. carinii adult antigens were tried in elisa and ihat for immunodiagnosis of bancroftian filariasis. both tests gave a higher positive reactions with microfilaraemic, clinical filariasis and endemic normal sera respectively. elisa using w. bancrofti microfilarial antigen appears to be more sensitive.19921578155
detection of microfilariae in cervical cytology. 19921580134
results of a safety trial on single-dose treatments with 400 mcg/kg of ivermectin in bancroftian filariasis.two groups of polynesian wuchereria bancrofti carriers, 17 females aged 21 to 84 years and 20 males aged 26 to 57 years, in whom microfilaraemia ranged from 1 to 10,121 mf/ml and from 1 to 6,484 mf/ml, respectively, were given a supervised singledose treatment with 400 mcg/kg of ivermectin. carriers were examined and questioned regarding their experience of adverse reactions, which were graded 0 to 3 according to severity, at 6, 12 and 24 hours and at 4 days after treatment. biological examinati ...19921293733
kinetics of circulating human igg4 after diethylcarbamazine and ivermectin treatment of bancroftian filariasis.patent filarial infections are associated with elevated levels of parasite-specific igg4. this study investigated the shifts of filarial-specific human igg and igg4 antibodies after diethylcarbamazine and ivermectin treatment of bancroftian filariasis. thirty adult haitians were treated first with a 1-mg clearing dose of ivermectin and then with either one or two 200-micrograms/kg doses of ivermectin or with 12 daily 6-mg/kg doses of diethylcarbamazine. posttreatment levels of antifilarial igg4 ...19921583340
on the infectivity of early third-stage brugia larvae isolated from the abdomen of aedes aegypti.third-stage larvae (l3) of wuchereria bancrofti, brugia malayi and b. pahangi are known to be recovered not only from the head and thorax but also from the abdomen of dissected vector mosquitoes. for epidemiological reasons, was of interest to determine whether l3 larvae from the abdomen of the vector would be infectious for the final host. early abdominal l3 larvae of b. pahangi isolated as early as on day 7 post-infection (p.i.) from aedes aegypti were injected s.c. into five male meriones ung ...19921584752
efficacy of bi-annual administration of dec in the control of bancroftian filariasis.the efficacy of bi-annual administration of dec at the dose of 6 mg/kg body weight was evaluated on the microfilaraemia prevalence, density and vector filarial infection rates. administration of four doses (4 x 6 mg/kg) of dec significantly (p < 0.05) reduced the microfilaria rate of the community from 6.02 per cent to 2.31 per cent, microfilaria density from 0.66 to 0.17 and infectivity rate of the vector population from 0.8 per cent to 0.39 per cent.19921344177
status of lymphatic filariasis in some select slum clusters of delhi.filaria surveys conducted in some select slum clusters namely hari nagar, yamuna pusht near vijaya ghat along the ring road and timarpur in delhi during 1989, 1991 and 1992 respectively, covering a population of approximately 5000 slum dwellers revealed the presence of bancroftian microfilaria (mf) carriers and disease cases. the mf and disease rates (per cent) in these three slum areas were in the order of 6.3, 2.2, 3.7 and 1.4, 0.5 and 0.1 respectively. the mf density varied from 3.1 to 12.3 p ...19921344178
the significance of low density microfilaraemia in the transmission of lymphatic filarial parasites.low density microfilaraemia (mf) is a density of circulating mf which is often undetected by standard survey techniques; it occurs naturally, after anti-filarial drug administration and after vector control. its occurrence in human populations is closely related to the observed mf frequency distributions in them, and it is an important cause of underestimation of mf prevalence rates in epidemiological surveys. in the present paper it is defined quantitatively as a count of less than 4 mf 20 micr ...19921348543
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