| incidence of endemic opisthorchis viverrini infection in a village in northeast thailand. | incidence, measured as the proportion of persons whose stools become positive within one year, was studied in endemic opisthorchis viverrini, the human liver fluke, in a northeastern thai village over a two-year period. incidence was higher in males than in females, especially in children under five years of age. it was at least 47% overall in the first year of the study, but declined to below 20% per year in the second. this is attributed to drying of a local water reservoir and decline in avai ... | 1985 | 4037181 |
| a comparison of a modified quick-kato technique and the stoll dilution method for field examination for opisthorchis viverrini eggs. | a modified quick-kato technique and the stoll dilution method for the diagnosis of opisthorchis viverrini infection were compared from the standpoints of both replicability and sensitivity. one stoll dilution preparation and two modified quick-kato smears were prepared from single stool specimens collected from 221 persons in a village in north-eastern thailand. stool examinations were made in duplicate for each technique by two examiners, and the averages of the raw egg counts were used to esti ... | 1983 | 6630991 |
| humoral immune responses in hamsters infected with opisthorchis viverrini. | the kinetics and nature of humoral immune responses to somatic and excretory-secretory (es) antigens were investigated in hamsters experimentally infected with different numbers of opisthorchis viverrini. es antigens were obtained from the in vitro culture of adult flukes and somatic antigens were aqueous extracts of adult flukes. antibodies in the serum and bile of infected animals were determined by the microhaemagglutination technique, using glutaraldehyde fixed sheep red blood cells sensitiz ... | 1983 | 6635763 |
| opisthorchis viverrini: the relationship between egg production, worm size and intensity of infection in the hamster. | worm recoveries, egg production and worm lengths were determined in golden syrian hamsters given between five and 200 opisthorchis viverrini metacercariae. animals given five, 10 or 20 metacercariae showed similar worm recovery rates of 38%, 36% and 37%, respectively, whereas animals given 50, 100 or 200 metacercariae gave recoveries of 49%, 65% and 54%, respectively. the distribution of worms between right, left and middle liver lobes was similar for animals with between one and 43 worms but di ... | 1983 | 6636282 |
| liver collagen in opisthorchis viverrini infected hamsters following praziquantel treatment. | the dose of praziquantel required to achieve a 100% worm reduction in o. viverrini infected hamsters was found to be 300 mg/kg body weight. the drug was administered orally for 1 day by dividing a total dose into 3 equal doses at 4 h interval. the effect of praziquantel treatment on liver collagen was followed by measuring liver collagen content in at various intervals after administration of the drug. a decrease in collagen content in the infected livers occurred within a few weeks following th ... | 1983 | 6658499 |
| cholangiocarcinoma associated with liver fluke infection: a preventable source of morbidity in asian immigrants. | in the far east infection with the liver flukes clonorchis sinensis and opisthorchis viverrini is the most frequently documented cause of cholangiocarcinoma. liver fluke infection in the united states remains a health problem for more than 500,000 southeast asian refugees who have immigrated to this country since 1975. recent surveys have revealed that up to 26% of asian immigrants have an active liver fluke infection. however, the common clinical manifestations of this condition, as well as the ... | 1986 | 3002170 |
| attempts to induce protective immunity in hamsters against infection by a liver fluke of man (opisthorchis viverrini). | the development of acquired resistance in opisthorchiasis was studied in hamsters experimentally infected with opisthorchis viverrini. the induction of protective immunity was attempted by first exposing adult female golden syrian hamsters to 1, 2 or 3 doses of infective metacercariae obtained from naturally infected cyprinoid fishes and then reinfecting them with 80 metacercariae. in other experiments, animals that were infected with 50 metacercariae were treated with praziquantel prior to bein ... | 1983 | 6835694 |
| liver collagen turnover in hamsters during infection by the human liver fluke, opisthorchis viverrini. | hamsters infected with opisthorchis viverrini, a liver fluke of man, showed an increased deposition of collagen in their livers. however, increased collagen breakdown as well as its synthesis were observed in the infected livers. thus, stimulated synthesis might be the main factor responsible for the net increase in collagen content. synthesis and degradation increased to a greater extent in short-term infection than in long-term infection whereas the hepatic collagen content was equally elevate ... | 1983 | 6877280 |
| effect of culture media on production of excretory-secretory products and egg output of opisthorchis viverrini in vitro. | adult opisthorchis viverrini were maintained metabolically active in vitro for an extended period in earle's basal medium (bme). the worms were most active during the first 7 to 10 days of incubation and the culturing fluids were found to react in immunodiffusion with antisera. the mean survival period and egg output per worm per day could be enhanced when bme was supplemented with 5% normal, human bile but not with the same concentration of bile ultrafiltrate. a similar degree of enhancement wa ... | 1982 | 6897077 |
| morbidity in relation to intensity of infection in opisthorchiasis viverrini: study of a community in khon kaen, thailand. | a study of the prevalence and intensity of opisthorchiasis viverrini in relation to morbidity as determined by standard medical examination was carried out in nong ranya, a small village containing 309 people in northeastern thailand. opisthorchis viverrini infection as determined by stoll's quantitative egg count method had an overall prevalence of 94% and reached 100% prevalence in most age groups above the age of 10 years. with respect to intensity, 6% were uninfected, 26% had light (less tha ... | 1982 | 6983303 |
| [clinical trial of niclofolan (bay 9015) in the treatment of human opisthorchiasis. apropos of 51 cases]. | 51 refugees from south east asia infected with opisthorchis viverrini or clonorchis sinensis were included in a therapeutic study with niclofolan (bay 9015) at a dose of 1 mg/kg body weight given three times at intervals of 48 hours. clinical and biological tolerance was carefully controlled and in all cases proved to be very good or excellent particularly in several patients with various haemoglobinopathies or erythrocytal enzymopathies (g-6-pd deficiency). of the total of 51 cases, 17 were cur ... | 1980 | 7014011 |
| studies on the chemotherapy of human opisthorchiasis: ii. clinical trial of niclofolan. | a clinical trial of niclofolan on human opisthorchiasis was carried out in 60 patients admitted to the hospital for tropical diseases. two dosage regimen, 2 mg and 3 mg per kg body weight repeated after a 72 hour interval was administered to 31 and 4 patients respectively. comparative evaluation with the placebo group of 25 patients was made by the stoll count on day 20, 40 and 60 after treatment. on day 60 percentage egg reduction of the treated and the placebo groups were similar. mild and tra ... | 1981 | 7020093 |
| serodiagnosis of opisthorchis viverrini infestation by an enzyme immuno-assay. | the application of an enzyme immuno-assay is evaluated with regard to antibody reactivity to opisthorchis viverrini antigen in 20 laotian patients and control groups including patients with other helminthic infections as well as persons without parasitic infestation. it is concluded that the enzyme immuno-assay indicates infection by o. viverrini. in certain circumstances, which are discussed, the test may serve for valuation of treatment. | 1982 | 7048673 |
| anti-p1, allohemagglutinins associated with clonorchis sinensis and opisthorchis viverrini infections in patients from southeast asia. | an automated assay of anti-p1 allohemagglutinins has been carried out on sera of 61 individuals from southeast asia : 28 with clonorchiasis, 18 with opisthorchiasis and 15 control subjects. anti-p1 activity was detected in 61% of the opisthorchiasis sera, 57% of the clonorchiasis sera and in 26.6% of the control subjects. their concentration, in the sera, was low in control subjects and exceptionally high in clonorchiasis and opisthorchiasis (up to 13 and 22 times the maximum concentration of th ... | 1982 | 7051340 |
| opisthorchis viverrini : intensity and rates of infection in cyprinoid fish from an endemic focus in northeast thailand. | the infection rates and monthly variations in intensities of opisthorchis viverrini infection in cyprinoid fish from an endemic focus in northeast thailand were investigated between april 1980 and march 1981. out of six species of cyprinoid fish examined, four were found to harbour o. viverrini metacercariae. all four species of infected fish showed high rates of infection. among the four species of o. viverrini infected fish, cycloccheilicthys apagon and puntius leiacanthus were found in abunda ... | 1982 | 7112213 |
| acquired resistance to opisthorchis viverrini in the hamster. | two groups of ten hamsters received a primary challenge of five opisthorchis viverrini metacercariae intragastrically (groups i and iii) and a third group was left untreated (group ii). 90 days after the primary challenge groups i and ii were given a further 50 metacercariae. animals were killed 35 days after the secondary challenge and worm burdens and faecal egg counts evaluated. compared with group ii animals, group i showed a 24.88% reduction in mean liver worm burden, a difference which was ... | 1982 | 7164147 |
| effects of physico-chemical factors on the infection of hamsters by metacercariae of opisthorchis viverrini. | | 1982 | 7170645 |
| opisthorchis viverrini: partial success in adoptively transferring immunity with spleen cells and serum in the hamster. | three groups of golden syrian hamsters received intraperitoneally either, (1) 1 x 10(7) spleen cells, (2) 0.5 ml. serum or, (3) 1 x 10(7) spleen cells plus 0.5 ml. serum, from donors infected 6 weeks previously with 25 opisthorchis viverrini metacercariae, one day before and at the same time as challenge with 25 metacercariae. three groups of control animals received the same quantities of spleen cells, serum or an admixture of both from normal non-infected donors in the same manner followed by ... | 1980 | 7217648 |
| potential health hazards of the water resources development: a health survey in the phitsanulok irrigation project, nan river basin, northern thailand. | a health survey was carried out among residents of 33 villages under the phitsanulok irrigation project area, nan river basin, northern thailand, whereby general health conditions were examined including intradermal tests for schistosomiasis japonica, stools for intestinal parasites and sera tested by circumoval precipitin test for antibodies to s. japonicum and by agglutination test for leptospiral infection. health investigations revealed that 913 (60.9%) of 1,499 people examined had experienc ... | 1980 | 7221699 |
| [praziquantel in the treatment of far eastern hepatic distomiasis caused by clonorchis sinensis or opisthorchis viverrini]. | forty-six patients infected with opistorchis viverrini and/or clonorchis sinensis, 24 males and 22 females, all laotian or vietnamese, aged 7 to 68, weighing 18 to 70 kg, were detected by parasitological stool examinations, numeration of eggs per gram of feces (e.p.g.). in this manner we observed 23 light infestations (1-999 e.p.g.), 20 moderate infestations (10,000-29,999 e.p.g.). before treatment, all these patients were submitted to a complete history and clinical examination with biological ... | 1981 | 7296740 |
| the in vitro effects of serum on the adults, metacercariae, and eggs of opisthorchis viverrini. | adult and juvenile opisthorchis viverrini liver-flukes incubated in hamster chronic infection serum (cis) developed tegumental precipitates which enclosed the entire parasite. adult worms appeared dead by 48 hours in cis and juvenile worms by 18 hours. no such reactions were observed in normal hamster serum (nhs). heat inactivation of cis at 56 degrees c abolished the precipitin reactivity against both adult and juvenile parasites though reactivity was regained upon addition of nhs or guinea-pig ... | 1981 | 7330943 |
| liver-fluke infection as an aetiological factor in bile-duct carcinoma of man. | this paper reviews, with particular reference to opisthorchis viverrini, the evidence that opisthorchiasis and clonorchiasis are aetiological factors in the causation of human bile-duct cancer, especially cholangiocarcinoma, and considers the other aetiological factors which may be operating. the epidemiology, pathology in man and experimental animals, and histogenesis of cholangiocarcinoma associated wtih liver-fluke infection are also considered. | 1981 | 6277052 |
| potentiation by the human liver fluke, opisthorchis viverrini, of the carcinogenic action of n-nitrosodimethylamine upon the biliary epithelium of the hamster. | | 1982 | 6295426 |
| praziquantel in clonorchiasis and opisthorchiasis. | a single stool examination revealed pathogenic intestinal parasites in 462 (58%) of 796 vietnamese and cambodian refugees. 56 (7.0%) were infected with clonorchis sinensis and/or opisthorchis viverrini. these patients received praziquantel in a dosage of 20 mg/kg bwt. p.day on 3 consecutive days. parasitological controls were completed after 12 months. no further excretion of eggs could be detected in 88% of the patients. concurrent infections with other trematodes and cestodes were also cured. ... | 1981 | 7345688 |
| detection of antibodies against opisthorchis viverrini in patients before and after treatment with praziquantel. | levels of antibody in sera of 78 patients with opisthorchiasis, 30 patients with other liver diseases, 10 patients with schistosomiasis and 30 healthy individuals were compared using three serodiagnostic tests, namely indirect haemagglutination (iha), enzyme-linked immunosorbent assay (elisa) and lectin immuno test (lit). the geometric mean reciprocal titer in sera of opisthorchiasis patients was significantly higher than patients with other diseases, patients with schistosomiasis and healthy in ... | 1988 | 3136551 |
| field studies on the bionomics of bithynia siamensis siamensis and the transmission of opisthorchis viverrini in bangna, bangkok, thailand. | a field study on the bionomics of bithynia siamensis siamensis and the transmission patterns of opisthorchis viverrini was carried out in bangna, bangkok, thailand, from may 1975 to april 1976. snail populations were found to fluctuate according to rainfall. during the high rainfall period where snail habitats were deep, snail populations recovered at the water surface were higher than those recovered on the mud bottom; during the low rainfall perod where snail habitats were shallow, snails were ... | 1980 | 7444576 |
| increased nitrosamine and nitrate biosynthesis mediated by nitric oxide synthase induced in hamsters infected with liver fluke (opisthorchis viverrini). | we previously reported that increased endogenous nitrosation in human subjects infected with the liver fluke opisthorchis viverrini in north-east thailand could be a risk factor for the development of cholangiocarcinoma. in the present study we examined our hypothesis that this increased endogenous nitrosation is mediated by nitric oxide (no) synthase induced by o. viverrini infestation. syrian golden hamsters experimentally infected with o. viverrini liver fluke excreted in the urine significan ... | 1994 | 7508824 |
| promotion of n-nitrosodimethylamine-initiated bile duct carcinogenesis in the hamster by the human liver fluke, opisthorchis viverrini. | four groups of male golden syrian hamsters were treated as follows: group i received 50 opisthorchis viverrini metacercariae followed 41 days later by a single oral dose of 1.6 mg n-nitrosodimethylamine (ndma). group ii received a single oral dose of 1.6 mg ndma followed 96 h later by 50 o. viverrini metacercariae, group iii received a single oral dose of 1.6 mg ndma only and group iv 50 o. viverrini metacercariae only. mortality was highest in group i and ii animals receiving both ndma and para ... | 1983 | 6307539 |
| possible protective immunity in human opisthorchiasis. | chronic infections with the liver flukes opisthorchis viverrini and clonorchis sinensis affect over 30 million people in southeastern asia. with ongoing exposure, reinfection readily occurs following curative treatment and cumulative infections result in significant morbidity and a predisposition to cholangiocarcinoma. though protective immunity has never been described in human opisthorchiasis, heterogeneity in worm burden occurs and a small number of exposed residents of endemic areas remain a ... | 1994 | 7526321 |
| therapy of fluke infections in the past. a review. | chemotherapy of trematode infections has been studied intensively in the past. the compounds so far used for the treatment of those parasitic infections were not satisfactory, however, because of their poor tolerability and/or their limited anthelmintic efficacy. since the introduction of tartar emetic in 1918, a number of antimonial as well as non-antimonial preparations have been used for the treatment of schistosomiasis. in the treatment of clonorchiasis with antimony preparations, gentian vi ... | 1984 | 6391498 |
| parasitological examinations and immunodiagnostic advances in fluke infections. | a reliable diagnosis of fluke infections should be based on the demonstration of parasite eggs in either faeces, duodenal liquid or sputum, depending on the type of infection. such an examination permits the identification of the species, and counting eggs establishes the intensity of infection. unfortunately this examination is useful only when intensity is rather high. the diagnosis of slight infections is uncertain and can vary considerably from one sample to another. in this area of parasito ... | 1984 | 6391499 |
| relationship between prevalence and intensity of opisthorchis viverrini infection, and clinical symptoms and signs in a rural community in north-east thailand. | in a large village in north-east thailand, the overall prevalence of opisthorchis viverrini infection (based on stoll's quantitative egg count) was 89.5% in a total population of 1651 individuals. the prevalence was 32% in children under 5 years, 90% in those aged 5-9 years, and averaged 95.6% in age groups above 10 years. the mean faecal egg output (indicative of intensity of infection) was highest in the 40-49-year age group and remained relatively constant through older ages. in all age group ... | 1984 | 6331907 |
| albendazole in the treatment of opisthorchiasis and concomitant intestinal helminthic infections. | a total of 52 adult patients with opisthorchiasis with or without concomitant intestinal helminthic infections were treated with albendazole at dosage regimens of 400 mg twice daily for 3 days (group i with 25 patients) and 7 days (group ii 27 patients). by concentration method with four examinations from two faecal specimens of each patient the cure rates and percentage egg reduction on day 30 in group i and group ii were 12% and 33%, 94 and 95 respectively; by stoll method the cure rates and p ... | 1984 | 6377514 |
| a clinical study of laotian refugees infected with clonorchis sinensis or opisthorchis viverrini. | twenty-six laotian refugees infected with clonorchis or opisthorchis liver flukes, and 15 uninfected controls, were assessed clinically by history, physical examination, serum 5'nucleotidase level, and serum immunoglobulin e level. ultrasound studies of the biliary tract were also done on a subset of six infected subjects. the results showed no differences between subjects and controls with respect to clinical symptoms, physical findings, 5'nucleotidase, or biliary ultrasound. mean serum ige lev ... | 1984 | 6391229 |
| evaluation of selected anthelmintic compounds for activity against opisthorchis viverrini. | in vitro and in vivo experiments were employed in the screening of potential anthelmintic agents against opisthorchis viverrini infection in hamsters. a few selected groups of compounds tested included those that are commercially available as well as those that are still being tested by various pharmaceutical firms. the compounds tested in the present study were praziquantel, amoscanate, albendazole, flubendazole, metrifonate, metronidazole and benzodiazepine derivatives. results from both in vi ... | 1984 | 6464190 |
| treatment of opisthorchiasis viverrini in hamsters with albendazole. | efficacy of many drugs against opisthorchis viverrini in man were shown by the percentages of egg reduction and the absence of egg in faeces. in some cases, however, the eggs reappeared after a period of time. the pathological changes of the bile duct could cause partial or even complete obstruction of the bile passages, thus resulting in the occurrence of natural egg reduction. albendazole, a broad spectrum anthelmintic was used to treat opisthorchiasis viverrini in hamsters. at dosage of 100 m ... | 1984 | 6523174 |
| association of liver fluke (opisthorchis viverrini) infestation with increased expression of cytochrome p450 and carcinogen metabolism in male hamster liver. | synergy between exposure to chemical carcinogens (nitrosamines) and infestation with the liver fluke opisthorchis viverrini has been demonstrated in a hamster model of hepatocarcinogenesis (flavell et al., carcinogenesis 4:927-930, 1983; thamavit et al., carcinogenesis 8:1351-1353, 1987). to elucidate the mechanisms of this interaction we tested the hypothesis that liver parasitism might influence the expression and activity of carcinogen metabolizing enzymes. we found that one, and perhaps more ... | 1994 | 7916996 |
| immunodepression in hamsters experimentally infected with opisthorchis viverrini. | hamsters experimentally infected with opisthorchis viverrini had significant depression of the antibody response to sheep red blood cells. the depression was more severe with prolonged infections. lymphoproliferative response to phytohaemagglutinin stimulation was also depressed when compared with uninfected controls. the immunodepression in these animals was abolished by anthelmintic treatment, suggesting that the immunodepressive effect is reversible and is associated with active infection. | 1987 | 3611709 |
| multi-infection with helminths in adults from northeast thailand as determined by post-treatment fecal examination of adult worms. | six hundred and eighty-one residents from 16 provinces in northeast thailand who had previously been found positive for opisthorchis viverrini and taenia spp. eggs were given 40 mg praziquantel (bayer, germany), per kg body weight. the total stool output for one to three days was collected and examined for adult worms. the prevalence of o. viverrini in this group was 92.4%. the intestinal flukes echinostoma malayanum, e. ilocanum and e. revolutum were found to be high in males, with 8.3%, 8.1% a ... | 1994 | 7939164 |
| studies on lophocercous cercariae from bithynia siamensis goniomphalus (prosobranchia: bithyniidae). | cercariae from bithynia siamensis goniomphalus were studied in an area endemic for opisthorchiasis. snails emitted different types of cercariae when shedding was induced by illumination. in addition to two lophocercous cercariae, a monostome cercaria (probably notocotylus sp), two different furcocercariae and a xiphidiocercaria were found. two similar types of lophocercous cercariae were distinguishable and one could be identified as opisthorchis viverrini. to date the cercaria of o. viverrini h ... | 1993 | 7939943 |
| pharmacokinetics of praziquantel in patients with opisthorchiasis. | the pharmacokinetics of praziquantel was investigated in 9 thai male patients with asymptomatic opisthorchiasis (stool positive) and 9 patients (6 males, 3 females) with moderately advanced infection (hepatomegaly). the geometric means of the pretreatment opisthorchis viverrini egg count in these patients were 2,950 vs 4,468 eggs per gram of stool. the results indicate the impairment of metabolism of praziquantel in the moderately advanced stage opisthorchiasis. the pharmacokinetics of the drug ... | 1993 | 7939947 |
| association of antibodies to opisthorchis viverrini with hepatobiliary disease in northeastern thailand. | the association between opisthorchis viverrini infection and hepatobiliary disease was studied in northeastern thailand. positive rates of o. viverrini infection and antibody titers to o. viverrini adult worm extracts, which were determined by enzyme-linked immunosorbent assay (elisa), were compared among four groups: thai patients with cholangiocarcinomas (chca), those with calculus cholecystitis (cclt), endemic area victims of traffic accidents, and japanese individuals used as negative contro ... | 1994 | 7943568 |
| cross-sectional study of opisthorchis viverrini infection and cholangiocarcinoma in communities within a high-risk area in northeast thailand. | we describe an innovative strategy to quantify risk of cancer associated with varying levels of exposure to chronic parasitic infection through the identification of asymptomatic cases of cholangiocarcinoma within a population-based survey of opisthorchis viverrini infection. stool samples from 12,311 adults over age 24 years from 85 villages in northeast thailand were examined for intensity of liver fluke infection. people from varying egg count categories were selected for ultrasound examinati ... | 1994 | 7960220 |
| parasite-associated morbidity: liver fluke infection and bile duct cancer in northeast thailand. | infection with the liver fluke, opisthorchis viverrini, remains a major public health problem in northeast thailand, where approximately one-third of the population is infected. the northeast region is largely populated by laos-descendent thais who enjoy eating raw fish, which harbour the infective stage of the fluke. the parasite has maintained its presence in the population despite the widespread use of praziquantel and dissemination of health education material throughout the region by vigoro ... | 1994 | 7982745 |
| opisthorchis viverrini infection in thailand: symptoms and signs of infection--a population-based study. | a population-based study of the clinical, laboratory and ultrasonographic findings in patients suffering from mild or moderate opisthorchiasis in prachinburi province, thailand was conducted in 1990-1992. the effectiveness of treatment with praziquantel at 40 mg/kg body weight was evaluated. after treatment, a long-lasting, marked improvement in the well-being of the study group was observed. symptoms common in opisthorchiasis infection decreased in intensity and the clinical response showed tot ... | 1994 | 7992340 |
| [prevalence of intestinal parasites among thais in israel]. | stool samples from 93 thais working in israel were examined for the presence of parasites. the overall prevalence of infection by 1 or more species was 74%. opisthorchis viverrini and hookworm were the most prevalent parasites (51.6% and 44.1%, respectively). they were also the parasites most frequently found in mixed infections; about a quarter of the workers were infected with both. other helminths found in only a few cases were: fasciolopsis buski (3 cases), taenia sp. (3), paragonimus sp. (1 ... | 1994 | 8034263 |
| opisthorchiasis control in northeast thailand: proposal for a new approach. | opisthorchiasis, i.e. infection with the liver fluke opisthorchis viverrini is an important health problem in thailand and adjacent laos, and its control is therefore of high priority. pilot projects have shown that after praziquantel treatment high re-infection rates occurred within a short period of time. to reduce re-infections it is proposed to couple the time of treatment to the seasonal transmission dynamics and thus treat the population in march when risk of infection for the snail and fi ... | 1994 | 8087151 |
| strong promoting effect of opisthorchis viverrini infection on dimethylnitrosamine-initiated hamster liver. | continuous administration of dimethylnitrosamine (dmn) to syrian hamsters infected with the liver fluke, opisthorchis viverrini (ov) results in a 100% incidence of cholangiocellular carcinomas. in a two-stage experiment, however, dosing with liver flukes caused only a few lesions to develop (flavel, d.j. and lucus, s.b. (1983) carcinogenesis, 4, 927]. to clarify this anomaly, syrian hamsters were initiated with 20 mg/kg dmn injected i.p. 19 days prior to 80 ov metacercaria infection. at 45 weeks ... | 1994 | 8180954 |
| identification and potential use of a soluble tumor antigen for the detection of liver-fluke-associated cholangiocarcinoma induced in a hamster model. | a liver-fluke-associated cholangiocarcinoma (cca), comparable to that occurring in humans, was induced by exposing opisthorchis viverrini-infected hamsters to dimethylnitrosamine (dmn). tumor masses were removed and histopathologically identified, then one portion was extracted for antigens used in the production of monoclonal antibodies (mabs). the remaining portions were used to establish cca cell lines. the antigens produced and secreted by these cell lines, as well as those originally presen ... | 1994 | 8194877 |
| promotion of cholangiocarcinogenesis in the hamster liver by bile duct ligation after dimethylnitrosamine initiation. | administration of hepatocarcinogenic nitrosamines before or after infection with the liver fluke, opisthorchis viverrini (ov), results in marked development of cholangiocellular and hepatocellular precancerous and cancerous lesions in the hamster liver. the promoting effects of ov are believed to be exerted either mechanically, chemically or immunologically. to test the influence of possible mechanical effects, syrian hamsters were initiated with a single i.p. injection of dimethylnitrosamine (d ... | 1993 | 8242874 |
| trematode infections. opisthorchiasis, clonorchiasis, fascioliasis, and paragonimiasis. | the parasitic diseases of the liver and lung are caused by trematodes or flukes--opisthorchis viverrini, o. felineus, fasciola hepatica, and paragonimus westermani. humans get infected by eating the second intermediate host of the fluke, for example, fish, crab, or water plant. the disease runs a chronic course. the diagnosis is made by the recover of eggs in stools or sputum, or by serodiagnosis. praziquantel is the drug of choice except in falcioliaisis. | 1993 | 8254167 |
| cholangiocarcinoma: epidemiology, mechanisms of carcinogenesis and prevention. | cholangiocarcinoma is a relatively rare cancer; worldwide it accounts for an estimated 15% of liver cancers. in most areas, the etiology is rather obscure, and identified risk factors such as hepatolithiasis, inflammatory bowel disease, and exposure to thorotrast can account for only a small proportion of cases. in certain areas of southeast and eastern asia, however, incidence rates are very high, and here there is a strong association with infection with the liver flukes clonorchis sinensis an ... | 1993 | 8268770 |
| opisthorchis viverrini: changes of the tegumental surface in newly excysted juvenile, first-week and adult flukes. | the tegumental surface of the newly excysted juvenile, first-week and adult stages of a human liver fluke, opisthorchis viverrini, was studied by scanning electron microscopy. the surface of the newly excysted juvenile is characterized by circumferentially arranged ridges alternated with troughs which bear rows of spines encircling the body. these spines are characterized by the shapes of their edges: the first type with serrated edge appears on the anterior part, and the second type with single ... | 1993 | 8314365 |
| reversal of biliary tract abnormalities associated with opisthorchis viverrini infection following praziquantel treatment. | we recently demonstrated a number of biliary tract abnormalities associated with moderate to heavy opisthorchis viverrini infection among 95 selected village residents in north-east thailand, who were treated with praziquantel immediately after initial examination and re-examined by abdominal ultrasonography 10 months later. dramatic improvement in the gall-bladder status of previously infected individuals was observed, including reduction of gall-bladder length and width and regained contractib ... | 1993 | 8337727 |
| imported opisthorchis viverrini and parasite infections from thai labourers in taiwan. | in order to determine the prevalence of intestinal parasites among labourers from thailand in taiwan, the stool samples from 1364 thai labourers were examined by the formalin-ether concentration method and 18.0% were found to be infected with one or more parasites. the infection rates of hookworm, opisthorchis viverrini, strongyloides stercoralis, enterobius vermicularis, ascaris lumbricoides, hymenolepis nana, capillaria philippinensis, schistosoma japonicum, echinostoma sp., entamoeba coli, gi ... | 1993 | 8354853 |
| precancerous lesions of the biliary tree. | malignant tumors may arise from any portion of the biliary tree. the term cholangiocarcinoma (cc) applies to both intra- and extrahepatic tumors. more than 95% of these tumors are adenocarcinomas. differentiation between cc and metastatic adenocarcinoma represents a difficult task for the pathologist. the presence of an intratumoral mini-ductal plate, and in situ carcinoma in bile ducts near the tumor and modulation from the bile duct towards parenchymal liver cells represent the major criteria ... | 1993 | 8389160 |
| ultrastructural hepatic alterations in hamsters and jirds after experimental infection with the liver fluke opisthorchis viverrini. | changes in the hepatocytes of male hamsters (mesocricetus auratus) and jirds (meriones unguiculatus) at 220 days after experimental infection with the liver fluke opisthorchis viverrini were studied by light and electron microscopy. the hepatocytes of the control group were characterized by an intracellular compartmentation. a globular nucleus was located centrally. the main features of the perinuclear zone were the cisternae of the rough endoplasmic reticulum (rer) and interjacent mitochondria, ... | 1993 | 8415540 |
| time-dependent modulation of liver lesion development in opisthorchis-infected syrian hamster by an antihelminthic drug, praziquantel. | in the north-east of thailand, repeated antihelminthic therapy has been introduced for control of the opisthorchiasis known to be a major risk factor for cholangiocellular carcinomas. what influence this may have on tumorigenesis, however, remains unclear. the effects of administration of praziquantel, an antihelminthic drug, at different time points subsequent to infection with opisthorchis viverrini (ov) on 2,2'-dihydroxy-di-n-propylnitrosamine (dhpn)-initiated lesion development in the liver ... | 1993 | 8463130 |
| field trials in the control of opisthorchis viverrini with an integrated programme in endemic areas of northeast thailand. | a control programme of opisthorchiasis was carried out for three years in three villages of northeast thailand in order to compare the effectiveness of two intervention measures. the intervention measures employed were to give praziquantel treatment to all infected people either once (village i) or twice (village ii) per year with the integration of regular health education and sanitation improvement. a control village (village iii) received no intervention during the study. sampling of the popu ... | 1993 | 8488064 |
| serum antibody responses in opisthorchiasis. | we evaluated an enzyme-linked immunosorbent assay using crude parasite homogenates as a diagnostic test for opisthorchis viverrini infection in humans. serum antibody (ab) responses to o. viverrini adult worm homogenate (awh) and metacercaria homogenate (mh) were studied in 83 infected residents of an opisthorchiasis-endemic area in thailand. elevated levels of ab persisted for over 1 year following curative treatment with praziquantel, and cross-reactivity to o. viverrini awh and mh antigens wa ... | 1995 | 8550296 |
| morphology and ultrastructure of the digestive gland of bithynia siamensis goniomphalus (prosobranchia: bithyniidae) and alterations induced by infection with the liver fluke opisthorchis viverrini (trematoda: digenea). | the morphology and ultrastructure of the digestive gland of bithynia siamensis goniomphalus and its alteration by infection with opisthorchis viverrini were investigated by light and electron microscopy. the digestive gland of b. s. goniomphalus was composed of three different cell types: digestive cells, excretory cells, and narrow cells. in infected animals the number of excretory cells increased dramatically. cellular injury in digestive cells as well as in excretory cells following the infec ... | 1995 | 8570585 |
| thiocyanate-independent nitrosation in humans with carcinogenic parasite infection. | infection with the liver fluke, opisthorchis viverrini, is a causative agent of cholangiocarcinoma. one possible contributing factor in this carcinogenesis is the chronic, local generation of nitric oxide by inflammatory cells expressing inducible nitric oxide synthase and the production of n-nitroso compounds via the reaction between amines and nitrosating agents derived from nitric oxide. our previous studies provided evidence that nitric oxide synthesis is elevated during human liver fluke in ... | 1996 | 8640916 |
| late complications of infection with opisthorchis viverrini. | | 1996 | 8775739 |
| intestinal parasites among the southeast asian laborers in taiwan during 1993-1994. | a large number of foreign laborers are now working in taiwan, the majority coming from southeast asia where parasitic infections are common. since 1992, monitoring the intestinal parasitic infections of these foreigners has been carried out, particularly to prevent the introduction of some of the parasites into taiwan. this paper reports results obtained during the year 1993-1994. | 1996 | 8803301 |
| cross-sectional patterns of hepatobiliary abnormalities and possible precursor conditions of cholangiocarcinoma associated with opisthorchis viverrini infection in humans. | the liver fluke, opisthorchis viverrini, is both highly prevalent and closely associated with cholangio-carcinoma in northeast thailand. this study measured associations between intensity of liver fluke infection and nonmalignant hepatobiliary disease diagnosed by ultrasonography among 1, 807 largely asymptomatic adult residents drawn from endemic communities. abnormalities significantly associated with intensity of infection included gallbladder enlargerment in all dimensions, presence of sludg ... | 1996 | 8842118 |
| equivocal evidence of complete carcinogenicity after repeated infection of syrian hamsters with opisthorchis viverrini. | the effects of repeated infection with opisthorchis viverrini on liver lesion development in male and female syrian hamsters were investigated over a 1-yr period. ten monthly intragastric applications of 50, 25, 13, or 0 parasite metacercariae resulted in pronounced proliferative and inflammatory lesions involving the first- and second-order ducts in response to the presence of adult worms. despite the development of small numbers of putative preneoplastic areas of cholangiofibrosis and morpholo ... | 1996 | 8864191 |
| cholangiocarcinoma in patients with opisthorchiasis. | cholangiocarcinoma is very common in areas endemic for the liver fluke opisthorchis viverrini. survival after surgical treatment of cholangiocarcinoma associated with opisthorchiasis was studied prospectively in 30 patients, all of whom resided in an endemic area. the median age was 52 (range 32-69) years and twenty-five patients were men. seven patients had their tumours removed, four with concomitant liver resection. twenty-two patients underwent palliative biliary bypass procedures to a segme ... | 1996 | 8869303 |
| induction of cytochrome p450 2a6 expression in humans by the carcinogenic parasite infection, opisthorchiasis viverrini. | the purpose of this study was to examine in vivo the activity of cytochrome p450 (cyp) 2a6, an enzyme capable of activating carcinogens, including n-nitrosodimethylamine, in humans with the carcinogenic liver fluke infection, opisthorchiasis viverrini, before and after treatment with the antiparasitic agent, praziquantel. coumarin hydroxylase activity of cyp 2a6 was assessed by administering a probe drug, coumarin, and measuring its metabolite, 7-hydroxycoumarin, in urines collected between 0-2 ... | 1996 | 8896890 |
| opisthorchis viverrini infection in thailand: studies on the morbidity of the infection and resolution following praziquantel treatment. | a community study on opisthorchiasis was conducted in prachinburi province in eastern thailand during 1990-1992. the morbidity from opisthorchiasis in the community and reversibility of biliary pathology following treatment with praziquantel at a single dose of 40 mg/kg were assessed by longitudinal investigations of clinical, laboratory, and ultrasonographic changes. a total of 913 voluntary subjects infected with opisthorchis viverrini were randomly selected for longitudinal study, and 579 sub ... | 1997 | 9129534 |
| prevalence of intestinal parasitic infection among children in two villages in lao pdr. | the prevalence of intestinal parasitic infection among 128 children under 15 years old in two villages in khammouane province, southeastern lao pdr, was investigated. overall prevalence of helminth infection was 77.3%; the prevalence was 64.8% in children under 6 years, 88.5% in those aged 6-10 years and 81.8% in the age group above 11 years. the prevalent helminths found in the subjects were ascaris lumbricoides (48.4%), trichuris trichiura (43.8%), hookworm (37.5%) and opisthorchis viverrini ( ... | 1996 | 9185270 |
| some immunological aspects of opisthorchiasis. | information regarding the development of acquired immunity in opisthorchiasis is particularly relevant if one is attempting eradication of the disease, e.g. by anthelmintic chemotherapy. clinical observations in patients with opisthorchiasis suggest that if acquired immunity develops at all, it is of low degree. using hamsters as experimental model, it was recently demonstrated that prior infection with opisthorchis viverrini failed to confer protection against reinfection, regardless of the siz ... | 1984 | 6542385 |
| opisthorchis viverrini: clinical experience with praziquantel in hospital for tropical diseases. | praziquantel (2-cyclohexylcarbonyl-1,2,3,6,7,11b-hexahydro-4h-pyrazino[2,1-a]++ +isoquinolin- 4-one, embay 8440, biltricide) has been used in 4853 patients with opisthorchis viverrini infection. 786 patients were treated as inpatients with extensive clinical evaluation and the rest were out-patients. a cure rate (evaluated with 5 faecal samples) of 100% was obtained in groups given 6 x 25 mg/kg on 2 days and 3 x 25 mg/kg on 1 day, while in groups given 2 x 25 mg/kg, 1 x 25 mg/kg and 1 x 40 mg/kg ... | 1984 | 6542386 |
| roentgenographically controlled healing of gallbladder lesions in opisthorchiasis after praziquantel treatment. | in a study, gallbladder lesions due to opisthorchis viverrini infection were controlled roentgenographically after treatment with praziquantel (2-cyclohexylcarbonyl-1,2,3,6,7,11b-hexahydro-4h-pyrazino[2,1-a] isoquinolin- 4-one, embay 8440, biltricide). the place of study was the khon kaen hospital, northeast thailand. included in the study were 20 patients with impairment of gallbladder function; 7 of them were males and 13 females. their age ranged from 27 to 60 years (mean 47.4), eggs per gram ... | 1984 | 6542387 |
| a pilot project for controlling o. viverrini infection in nong wai, northeast thailand, by applying praziquantel and other measures. | in a pilot control project of opisthorchis viverrini in the village of nong wai irrigation area, khon kaen, northeast thailand, selected population based chemotherapy with a single dose of 40 mg/kg of praziquantel (2 - cyclohexylcarbonyl-1,2,3,6,7,11 b-hexahydro-4h-pyrazino [2,1-a]isoquinolin-4-one,embay8440,biltricide) was given to the stool positive cases once a year for a period of 3 years commencing may 1981. two villages (pop 942) with a prevalence of 58.3% were used as tests villages and a ... | 1984 | 6542402 |
| field studies on the transmission of the human liver fluke, opisthorchis viverrini, in northeast thailand: population changes of the snail intermediate host. | | 1986 | 3781736 |
| studies on the chemotherapy of human opisthorchiasis: effective dose of praziquantel in heavy infection. | ninety-six patients who had heavy opisthorchis viverrini infection were studied. egg count per gram of faeces ranged from 10,800 to 139,000 (mean 26,044.3). praziquantel 50 mg per kg body weight was given after a morning meal. 68 patients completed the follow up period of 60 days. the cure rate was 97.0%. the side-effects occurred in 61 patients (89.7%). the common side effects were diarrhoea, dizziness, sleepiness, epigastric pain, headache, nausea and anorexia. these side-effects were mild and ... | 1985 | 3906918 |
| enzyme-linked immunosorbent assay for detection of opisthorchis viverrini infection. | antibodies to o. viverrini in the sera of people from endemic and non-endemic areas were investigated using indirect elisa technique. for the patients from the endemic area, 92.8% who passed eggs in the stool were found to be positive for o. viverrini antibody. in addition, 46.5% of the people who did not pass eggs in the stool were also found to have low titer of o. viverrini antibody. on the other hand only 2.4% of the people from the non-endemic area with other intestinal parasite infections ... | 1985 | 4071196 |
| clinical features in severe opisthorchiasis viverrini. | clinical features of severe opisthorchiasis were studied in 88 patients. the ratio of males to females was 6.3 : 1; 75% were over 40 years old. the presenting symptoms were obstructive jaundice, 25% associated with secondary infection of biliary system; cholangitis and cholecystitis; intraabdominal mass, which was enlarged liver; 18% had palpable gallbladder; 18% had adenocarcinoma of the bile duct. high bilirubin was found in 46% of cases, high alkaline phosphatase in 80%; elevation of serum tr ... | 1985 | 4095605 |
| report of episthmium caninum (verma, 1935) yamaguti, 1958 (digenea: echinostomatidae) in man. | the first finding of episthmium sp. in man is reported. characteristics of the worm are given in detail. the infestation is an accidental one acquired from consuming raw fish, which serves as an intermediate host of opisthorchis viverrini. the pathogenic role of episthmium sp. in man needs further investigations. | 1985 | 4095615 |
| liver carcinoma and liver fluke infection. | the neoplastic transformation of bile duct changes induced by infections due to clonorchis sinensis or opisthorchis viverrini has been persistently debated because of the difficulty to establish the causative relation between those trematode infections and the development of primary carcinoma of the liver (pcal) which has been found in hong kong, china, korea and thailand. the following observations, however, clearly support the hypothesis that clonorchiasis and opisthorchiasis are basic etiolog ... | 1984 | 6095876 |
| alpha 1-antitrypsin, alpha 1-antichymotrypsin and alpha 2-macroglobulin in human liver fluke (opisthorchiasis). | in mild and moderate opisthorchis viverrini infection it is difficult to find clinical signs for diagnosis. also routine clinical chemical reactions often fail to demonstrate changes in these patients. this study showed a significant increase of serum proteinase inhibitors, alpha 2-macroglobulin, alpha 1-antitrypsin and alpha 1-antichymotrypsin in patients suffering from o. viverrini infection compared with healthy controls. the increase of proteinase inhibitors together with other biochemical l ... | 1982 | 6182657 |
| treatment of opisthorchis viverrini with mebendazole. | thirty-six hospitalized thais (21 males, 15 females, 9 to 63 years of age) with opisthorchis viverrini infection were treated with mebendazole in dosages of 20 mg/kg body weight for 4 weeks and 30 mg/kg body weight for 3 or 4 weeks. the drug was found to be effective when given in dosages of 30 mg/kg body weight for 3 to 4 weeks only a few eggs being found in the stool of one of 27 persons at 3 or 4 weeks post-treatment. in follow-up examination at 6 months, only two of 15 persons available were ... | 1981 | 7344109 |
| field trial of praziquantel in human opisthorchiasis in thailand. | praziquantel is an excellent drug for treatment against opisthorchis viverrini infection. sixty cases of opisthorchis viverrini infection were treated with praziquantel, 3 x 25 mg/kg body weight for one day, except one case that was retreated with the same regimen at one month after the first treatment, and the cure rate was 100.0% at one year after the treatment. the side effects of the drug were headache, lassitude, sleepiness, and diarrhoea, but most of these were only in mild degree. other l ... | 1981 | 7344110 |
| [intestinal helminths in bangkok stray dogs and their role in public health (author's tranls)]. | feces examination of 107 stray dogs in bangkok, thailand, on intestinal helminths showed the following rates: hookworms 100%, trichuris vulpis 54.2%, toxocara canis 6.5%, gnathostoma spinigerum 2.8%, spirocerca lupi 17.8%, diphyllobothrium mansoni 1.9%, and opisthorchis viverrini 1.9%. out of these species hockworms (especially a. braziliense), t. canis, g. spinigerum, and d. mansoni are important sources of human cutaneous or visceral larva migrans, the latter two being special parasites of the ... | 1980 | 7435002 |
| liver fluke infection and cholangiocarcinoma: model of endogenous nitric oxide and extragastric nitrosation in human carcinogenesis. | cancers arising during bacterial, viral and parasitic infection provide useful models to investigate the link between inflammation and carcinogenesis. because the inflammatory agent is known, relationships between immune responses, the production of dna-damaging agents, such as nitric oxide, oxygen radicles and n-nitroso compounds, and cancer risk can be explored. this paper first describes the close relationship between infection with the liver fluke, opisthorchis viverrini, and cholangiocarcin ... | 1994 | 7510035 |
| [cholangiolar carcinoma]. | cholangiocarcinoma affects both sexes equally, is more prevalent in individuals age 50 to 70. associations with many other diseases are known, in particular with primary sclerosing cholangitis, ulcerative colitis and parasitic infestation (clonorchis sinensis, opisthorchis viverrini). about 95% are histopathologically classified as adenocarcinoma. the localisation of the tumor determines clinical course and prognosis. if the tumor is located above the hepatic duct bifurcation, only one side of t ... | 1994 | 7531268 |
| morphology and ultrastructure of the redia and pre-emergent cercaria of opisthorchis viverrini (trematoda: digenea) in the intermediate host bithynia siamensis goniomphalus (prosobranchia: bithyniidae)]. | the intramolluscan stages of the human liver fluke opisthorchis viverrini were investigated by light, scanning, and transmission electron microscopy. the morphology of the tegument, and the excretory system of the redia and pre-emergent cercaria as well as glandular cells, tail structure, and the eye spots of the cercarial stage are described. possible environmental adaptations are discussed. the redia is characterized by a tegument which is folded concentrically and covered with densely arrange ... | 1995 | 7550441 |
| opisthorchis viverrini: the tegumental cytoskeleton. | the tegumental cytoskeleton of opisthorchis viverrini was observed using both conventional transmission electron microscopy and triton x-100 extraction. the cytoskeletal elements of the newly excysted juveniles, first-week and adult stages are composed of 2 components: firstly, the network of knobbed fibres designated as microtrabeculae which form the principal scaffold of the cytoplasm; and secondly, the microtubules. the microtrabeculae are more densely packed in the newly excysted juveniles a ... | 1995 | 7558564 |
| serum antibody response to opisthorchis viverrini antigen as a marker for opisthorchiasis-associated cholangiocarcinoma. | the liver flukes opisthorchis viverrini and clonorchis sinensis chronically infect over 30 million people in south-eastern asia, resulting in significant morbidity and a predisposition to cholangiocarcinoma (cca). liver fluke-associated cca carries a poor prognosis, partly because it is often detected at a late and advanced stage. the development of improved diagnostic methods, particularly for early cca, may improve chances of survival and cure. accordingly, we explored the use of immunological ... | 1994 | 7570848 |
| evaluation of a monoclonal antibody-based enzyme linked immunosorbent assay for the diagnosis of opisthorchis viverrini infection in an endemic area. | a monoclonal antibody-based enzyme-linked immunosorbent assay (mab-elisa) was evaluated for its potential in the diagnosis of opisthorchiasis in an area endemic for opisthorchis viverrini infection. the method, based on the detection of the 89-kd o. viverrini metabolic antigen in the feces (coproantigen), was previously estimated to be sensitive enough to detect antigen excreted by a single mature fluke. in the present study, fecal specimens from 207 apparently healthy villagers in northeastern ... | 1995 | 7611558 |
| infection with liver flukes (opisthorchis viverrini, opisthorchis felineus and clonorchis sinensis). | | 1994 | 7715069 |
| changes in serum antibodies to opisthorchis viverrini in humans and hamsters following treatment of opisthorchiasis. | | 1994 | 7872997 |
| opisthorchis viverrini: ultrastructure of the tegument of the first-week juveniles and adult flukes. | the tegument of one-week-old and adult flukes of opisthorchis viverrini were studied by transmission electron microscopy. the tegument of both stages is composed of a syncytium formed by processes of the tegumental cells lying underneath the outer-circular and the inner-longitudinal muscle layers. the tegument is bounded by trilaminate outer and inner membranes; the former is coated with a thin glycocalyx, while the latter has short basal infoldings. there are 4 forms of tegumental granules, nam ... | 1994 | 7928062 |
| alterations of the surface tegument of opisthorchis viverrini exposed to praziquantel in vitro and in vivo. | the in vitro and in vivo effects of praziquantel on the ultrastructural surface of opisthorchis viverrini were investigated using scanning electronmicroscopy. for the in vitro study, adult flukes were collected from experimentally infected hamsters, and were incubated for various time intervals at 37 degrees c in earle's basal medium containing praziquantel at final concentrations of 0.01-100 micrograms/ml. for the in vivo study, flukes were collected from the biliary system of experimentally in ... | 1984 | 6740385 |
| seasonal variation of opisthorchis viverrini infection in cyprinoid fish in north-east thailand: implications for parasite control and food safety. | reported is the seasonal pattern of opisthorchis viverrini metacercariae in cyprinoid fish in north-east thailand. samples of fish were collected in 1991-92 at monthly intervals from two areas-khon kaen province, where the opisthorchiasis transmission rate was high, and mahasarakham province, where the rate was low. metacercarial loads in both study areas had similar seasonal patterns. high burdens occurred in the late rainy season and winter (july to january) with low burdens during the summer ... | 1997 | 9185364 |
| opisthorchis viverrini: effect of praziquantel on the adult tegument. | ultrastructural changes of the tegument of adult liver flukes, opisthorchis viverrini, after in vitro incubation in minimal essential medium containing 0, 0.1, 1.0 and 10.0 micrograms/ml of anthelminthic praziquantel for 5, 15, 30, 45 and 60 minutes were investigated by scanning (sem) and transmission (tem) electron microscopy. sem observations showed that the surface damage was composed of blebbing due to the swelling of microvilli, followed later by the disruption of these structures to form l ... | 1996 | 9279994 |
| opisthorchis viverrini: the effects of colchicine and cytochalasin b on the adult tegument. | the roles of the tegumental cytoskeleton were tested by treating adult flukes with colchicine and cytochalasin b. following a short incubation period (10-20 minutes), colchicine disrupted microtubules in the tegumental cells' processes which, in turn, affected the transport of dense granules from the cells' soma to the tegument; as a result some of these granules were fused together to form membrane-bound vacuoles. in addition, at many spots microtrabeculae were also depolymerized, which resulte ... | 1996 | 9279995 |
| parasites elicited cross-reacting antibodies to opisthorchis viverrini. | two batches of crude antigens extracted from adult opisthorchis viverrini worms were compared. one was derived from adult worms harvested from the livers of laboratory infected hamsters and another was obtained from worms sedimented from the faeces of opisthorchiasis patients following treatment with praziquantel. sds-page and coomassie brilliant blue staining revealed that the two preparations had similar protein components of which the predominant ones were the 17-18 kda doublet. the antigens ... | 1997 | 9346276 |
| relationships between the synthesis of n-nitrosodimethylamine and immune responses to chronic infection with the carcinogenic parasite, opisthorchis viverrini, in men. | this study investigated the relationship between immune responses to infection with the liver fluke, opisthorchis viverrini, and the synthesis of the carcinogen, n-nitrosodimethylamine (ndma) in humans. it also examined associations between synthesis of nitric oxide (no) and nitrosation of amines, in vivo. antibody and t cell responses to fluke antigens and post-alcohol urinary ndma excretion were assessed among three groups of 40-50 men with no, moderate and heavy liver fluke infection. markers ... | 1998 | 9525284 |
| parasitic infections among southeast asian labourers in taiwan: a long-term study. | parasitic infections have been reported to be relatively common among the southeast asian labourers in taiwan. this study, conducted in 1992-6, was designed to determine the temporal changes of the prevalence. faecal specimens were examined by the formalin-ethyl acetate sedimentation technique and blood samples screened using the quantitative buffy coat technique and confirmed by giemsa stained blood smear. the overall prevalence of intestinal parasitic infections was 10.3%. the annual prevalenc ... | 1998 | 9528821 |