| ultrastructural studies of sarcocystis cruzi (hasselmann, 1926) wenyon, 1926 infection in cattle (bos taurus): philippine cases. | this paper documents the first report of sarcocysti s cruzi infection in domesticated cattle (bos taurus) in the philippines. fusiform-shaped microscopic sarcocysts (183-578 microns long and 20-98 microns wide) with distinct septae were found in the skeletal, striated and heart muscle. the sarcocyst wall or parasitophorous vacuolar membrane, 1.37-2.75 microns thick consisted of closely-packed villar protrusions 80-400 nm in dm. middle and distal segments of vp were bent approximately 90 degrees ... | 2001 | 11584756 |
| experimental induction of the two-host life cycle of sarcocystis cruzi between dogs and korean native calves. | eight dogs were experimentally infected with sarcocystis by oral inoculation of cardiac muscle from naturally infected cattle. the infected dogs commenced discharging of sporocysts in the feces after 10 to 12 days of inoculation, and continued until 20 and 35 days after inoculation. three dogs were reinfected with cardiac muscle from the naturally infected cattle. sporocysts reappeared in the feces on 12 to 13 days after reinfection. sarcocystis sporocysts collected from the experimentally infec ... | 2001 | 11590912 |
| sensitive and specific identification of neospora caninum infection of cattle based on detection of serum antibodies to recombinant ncp29. | neosporosis is an economically important disease of dairy cattle caused by the protozoan neospora caninum. diagnostic tests for neosporosis are complicated by the potential for cross-reaction of antibodies to antigens that are similar between n. caninum and closely related parasites toxoplasma gondii and sarcocystis cruzi. to provide a sensitive and specific assay for detecting antibodies to n. caninum in the serum of infected animals, we have investigated a recombinant form of the antigen known ... | 2002 | 11986268 |
| effect of progressive cachectic parasitism and growth hormone treatment on hepatic 5'-deiodinase activity in calves. | thyroid status is compromised in a variety of acute and chronic infections. conversion of thyroxine (t(4)) into the metabolically active hormone, triiodothyronine (t(3)), is catalyzed by 5'-deiodinase (5'd) mainly in extrathyroidal tissues. the objective of this study was to examine the effect of protozoan parasitic infection (sarcocystis cruzi) on hepatic 5'd (type i) activity and plasma concentrations of t(3) and t(4) in placebo- or bovine gh (bgh)-injected calves. holstein bull calves (127.5+ ... | 2002 | 12044611 |
| a pcr-based rflp analysis of sarcocystis cruzi (protozoa: sarcocystidae) in yunnan province, pr china, reveals the water buffalo (bubalus bubalis) as a natural intermediate host. | a polymerase chain reaction-based restriction fragment length polymorphism (rflp) approach is used to examine sarcocystis cruzi-like taxa from the atypical intermediate host, water buffalo, in yunnan, people's republic of china. the loci examined lie within the 18s rrna gene. a total of 15 water buffalo isolates are compared with those of 10 s. cruzi from cattle. rflp patterns for the s. cruzi isolates from cattle and the s. cruzi-like taxon from water buffalo are found to be identical with all ... | 2002 | 12537125 |
| [lesions in cross-breed pregnant cows, experimentally infected with sarcocystis cruzi (hasselmann, 1923) wenyon, 1926 (apicomplexa: sarcocytidae)]. | to identify pathologic alterations, sixteen cows, in the last 3 months of gestation, all cross-breed, holstein x zebu, serum positive for sarcocystis, were selected and divided into four groups of four animals each. the first group was considered as control and the other three groups were experimentally infected with 1x10(4) (2nd group), 5x10(4) (3rd group) and 1x10(5) (4th group) sporocysts obtained from dog's feces. the gross lesions in the cows were consisted of pale mucous membranes, palenes ... | 2005 | 16153349 |
| chronic polymyositis associated with disseminated sarcocystosis in a captive-born rhesus macaque. | a 2-year-old, captive-born, clinically healthy male, rhesus macaque, was euthanatized as part of an experimental study. at necropsy, diffuse pale streaking of the trunk, lumbar, and limb muscles were noted macroscopically. on histology, numerous elongated cysts that contained crescent-shaped basophilic spores were found in the fibers of skeletal muscles. scattered affected myofibers were degenerate and accompanied by eosinophilic-to-granulomatous inflammation. sarcocysts had prominent villus-lik ... | 2007 | 17846244 |
| diagnosis of sarcocystis cruzi, neospora caninum, and toxoplasma gondii infections in cattle. | the aim of the study was to diagnose sarcocystis sp. infections in cattle and to detect coinfections by toxoplasma gondii and/or neospora caninum. blood, diaphragm, esophagus, and myocardium from 90 beef cattle from argentina were collected. histopathological, immunohistochemical, polymerase chain reaction assays, and direct microscopical examination were carried out. sarcocysts from myocardium were measured and counted. indirect fluorescent antibody test (ifat) for the three protozoans was perf ... | 2008 | 18066600 |
| restricted genetic diversity in the ubiquitous cattle parasite, sarcocystis cruzi. | although parasites of the genus sarcocystis have likely cycled between bovine herbivores and canine carnivores for tens of millions of years, humans may have profoundly influenced the ecology and evolution of those prevalent in domesticated dogs and cattle. to preliminarily assess the possibility of such anthropogenic effects, we surveyed genetic variation in conserved (18s small subunit) and variable (its-1) portions of ribosomal dna from a large sample of sarcocystis cruzi occurring in taurine ... | 2008 | 18501682 |
| frequency of horizontal and vertical transmission for sarcocystis cruzi and neospora caninum in dairy cattle. | sarcocystis cruzi and neospora caninum infections in cattle are common throughout the world, and cause important economical losses. n. caninum can be transmitted horizontally by ingestion of oocysts or vertically from the infected dam to the fetus via the placenta. vertical transmission for s. cruzi is infrequent and horizontal transmission is considered the most important route of infection. the objectives of this study were to evaluate the frequency of horizontal and vertical transmission for ... | 2009 | 19070964 |
| non-invasive methods for identifying oocysts of sarcocystis spp. from definitive hosts. | because the excreted sporocysts and/or oocysts of various species of sarcocystis may not be discriminated morphologically, we sought to validate a diagnostic technique based on variation in the 18s rdna sequence. oocysts and/or sporocysts from three taxa of sarcocystis were collected from human, feline, and canine definitive hosts that had fed upon meats infected with the muscle cysts of sarcocystis hominis, sarcocystis fusiformis and a species of sarcocystis from water buffalo that could not be ... | 2009 | 19336258 |
| [sarcocystis cruzi (protozoa: apicomplexa: sarcocystiidae) infection in european bison (bison bonasus) from białowieza forest, poland]. | samples of oesophagus, diaphragm and heart muscles were taken from one european bison from białowieza forest during seasonal european bison elimination in 2008. five gram of each muscle was examined by staining small samples in 0.2% aqueous solution of methylene blue. after that they were placed between compressor glasses and examined under a dissecting microscope in order to detect sarcocystis sp. infection. all sarcocysts observed were counted. the 5-g sample of the heart muscle yielded a tota ... | 2009 | 19579782 |
| diagnosis of sarcocystis spp. in cattle (bos taurus) and water buffalo (bubalus bubalis) in northern vietnam. | our aim was to develop a method for species diagnosis and to obtain data on the prevalence of sarcocystis infections in cattle and water buffalo in the son la province of northern vietnam. meat samples of naturally infected animals were examined by light and electron microscopy as well as by molecular methods. a pcr of part of the 18s rdna gene followed by rflp analysis was modified to detect infections with different sarcocystis spp. in cattle and water buffaloes slaughtered in the son la provi ... | 2009 | 19783101 |
| review of zoonotic parasites in medical and veterinary fields in the republic of korea. | zoonotic parasites are animal parasites that can infect humans. the major zoonotic protozoa in the republic of korea are babesia bovis, chilomastix mesnili, cryptosporidium parvum, endolimax nana, entamoeba coli, entamoeba hitolytica, giardia lamblia, iodamoeba bütschlii, pneumocystis carinii, sarcocystis cruzi, and toxoplasma gondii. the major zoonotic helminths in korea include trematodes, cestodes, and nematodes. trematodes are clonorchis sinensis, echinostoma hortense, echinostoma spp., fasc ... | 2009 | 19885329 |
| [evaluation of small intestine and mesenteric lymph nodes of dogs (canis familiaris) experimentally infected by sarcocystis cruzi (hasselman, 1923) wenyon, 1926 (apicomplexa: sarcocystidae)]. | species of the genus sarcocystis are considered as important parasites of domestic animals. dogs are considered as definitive host for a variety species of sarcocystis, when they fed on intermediated host tissues cysts shed sporocysts in their feces. grinded bovine cardiac muscles, positive for sarcocystis cruzi was given to two puppies free of coccidia. both animals shed sporocysts in their feces at 12 days after infection (dai). these sporocysts measured 17.44 +/- 0.82 11.60 +/- 0.66 mum with ... | 2008 | 20059872 |
| meningoencephalitis associated with sarcocystis spp. in a free-living japanese raccoon dog (nyctereutes procyonoides viverrinus). | a free-living, young adult, male japanese raccoon dog (nyctereutes procyonoides viverrinus) was rescued in gifu, japan in march 2009. the animal was weak and emaciated with neurological signs that included head tilt, tremor and tic. the brain showed no gross abnormality at necropsy, but microscopically there was severe meningoencephalitis associated with protozoa, which were morphologically consistent with the asexual developmental stage of sarcocystis spp. the protozoa were immunohistochemicall ... | 2010 | 20163802 |
| comparative evaluation of immunofluorescent antibody and new immunoblot tests for the specific detection of antibodies against besnoitia besnoiti tachyzoites and bradyzoites in bovine sera. | besnoitia besnoiti, an apicomplexan parasite causes economically important disease in cattle in many countries of africa and asia is re-emerging in europe. serological identification of infected cattle is important because introduction of these animals into naive herds seems to play a major role in the transmission of the parasite. we report new, simplified immunoblot-based serological tests for the detection of b. besnoiti-specific antibodies. antigens were used under non-reducing conditions in ... | 2010 | 20378250 |
| prevalence of thin-walled sarcocystis cruzi and thick-walled sarcocystis hirsuta or sarcocystis hominis from cattle in iran. | bovine sarcocystosis is caused by sarcocystis cruzi and is known to cause considerable morbidity and mortality in cattle. this species is distributed worldwide in cattle and is the most prevalent of the sarcocystis species infecting cattle. there is high infection rate of sarcocyst in cattle in iran, but to our knowledge, there is no study about identification of sarcocystis species. this work aimed to survey prevalence of s. cruzi cyst in slaughtered cattle of isfahan, iran. in this study, esop ... | 2010 | 20379775 |
| sarcocystis cruzi: comparative studies confirm natural infections of buffaloes. | controversy exists concerning whether cattle and water buffalo sustain infections with cysts of distinct arrays of species in the genus sarcocystis. in particular, morphologically similar parasites have been alternately ascribed to sarcocystis cruzi or to sarcocystis levinei, depending on their occurrence in cattle or water buffalo. we used light and transmission electron microscopy, genetic analysis, and experimental infections of definitive canine hosts to determine whether consistent differen ... | 2010 | 20971107 |
| prevalence of sarcocystis spp. in argentinean cattle. | sarcocystis cruzi, s. hirsuta and s. hominis are apicomplexan parasites that affect cattle worldwide with variable prevalence. the aim of the present study was to evaluate the prevalence of sarcocystis spp. in argentinean cattle comparing microscopic fresh examination and molecular methods. blood, myocardium and loin samples were collected in five slaughterhouses from a total of 380 bovines. origin of animals was representative of the major beef cattle production area of argentina. samples were ... | 2010 | 21168276 |
| detection and differentiation of coccidian oocysts by real-time pcr and melting curve analysis. | abstract rapid and reliable detection and identification of coccidian oocysts are essential for animal health and food-borne disease outbreak investigations. traditional microscopy and morphological techniques can identify large and unique oocysts, but are often subjective and require parasitological expertise. the objective of this study was to develop a real-time quantitative pcr (qpcr) assay with melting curve analysis (mca) to detect, differentiate, and identify dna from coccidian species of ... | 2011 | 21506835 |
| detection of a morphogenetically novel sarcocystis hominis-like in the context of a prevalence study in semi-intensively bred cattle in italy. | the aim of this study was to determine the prevalence of sarcosporidiosis in semi-intensively bred cattle in northwestern italy. a diagnostic protocol was setup in which infected animals were identified by rapid histological examination of the esophagus, diaphragm, and heart and the detected sarcocystis spp. were subsequently typed using conventional electron microscopy in combination with molecular techniques. sarcosporidia cysts were detected in 78.1% of the animals and were seen most often in ... | 2011 | 21556683 |
| detection of sarcocystis spp. in cattle (bos taurus) and water buffaloes (bubalus bubalis) in iran by pcr-rflp. | sarcocystis species are cyst-forming intracellular protozoan parasites. cattle are mainly infected with sarcocystis cruzi, sarcocystis hominis and sarcocystis hirsuta. water buffaloes are intermediate hosts for sarcocystis fusiformis, sarcocystis levinei (s. cruzi-like species), sarcocystis dubeyi, sarcocystis sinensis (s. hominis-like species) and sarcocystis buffalonis (s. hirsuta- like species). the aim of this study was identification of sarcocystis spp. in slaughtered cattle and water buffa ... | 2014 | 26688630 |
| the parasitic fauna of the european bison (bison bonasus) (linnaeus, 1758) and their impact on the conservation. part 1. the summarising list of parasites noted. | during the current century, 88 species of parasites have been recorded in bison bonasus. these are 22 species of protozoa (trypanosoma wrublewskii, t. theileri, giardia sp., sarcocystis cruzi, s. hirsuta, s. hominis, s. fusiformis, neospora caninum, toxoplasma gondii, cryptosporidium sp., eimeria cylindrica, e. subspherica, e. bovis, e. zuernii, e. canadensis, e. ellipsoidalis, e. alabamensis, e. bukidnonensis, e. auburnensis, e. pellita, e. brasiliensis, babesia divergens), 4 trematodes species ... | 2014 | 25119348 |
| prevalence of enteric protozoan oocysts with special reference to sarcocystis cruzi among fecal samples of diarrheic immunodeficient patients in iran. | the genus sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. it might be expected that species for which humans are the final host (sarcocystis hominis and sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. this study aimed to address how to detect and differentiate sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patient ... | 2016 | 27417091 |
| pathology, immunohistochemistry, and ultrastructural findings associated with neurological sarcocystosis in cattle. | paraffin-embedded blocks of brain of a nine months old bull calf that died of neurological signs in 1982 in germany were restudied. numerous schizonts and merozoites were found associated with extensive but focal necrosis and severe meningoencephalitis. developing stages of schizonts as well as free merozoites were identified. the schizonts were primarily in perivascular areas. ultrastructurally, schizonts were seen both in capillaries and in extravascular space. merozoites were often concentrat ... | 2016 | 27198793 |
| molecular differentiation of sarcocystis buffalonis and sarcocystis levinei in water buffaloes (bubalus bubalis) from sarcocystis hirsuta and sarcocystis cruzi in cattle (bos taurus). | the purpose of the present study was to obtain sarcocysts of sarcocystis buffalonis and sarcocystis levinei from water buffaloes and characterize the isolates by molecular methods in order to determine whether the two species were genetically different from sarcocystis hirsuta and sarcocystis cruzi, respectively, from cattle, which had been characterized before. about 35 macroscopically visible (3-4 × 1-2 mm) and 20 barely visible (1-3 × 0.2 mm) sarcocysts were excised from the esophagus of 18 n ... | 2016 | 26979729 |
| short communication: genetic variants of sarcocystis cruzi in infected malaysian cattle based on 18s rdna. | sarcocystis species are pathogenic parasites that infect a wide range of animals, including cattle. a high prevalence of cattle sarcocystosis has been reported worldwide, but its status is unknown in malaysia. this study focused on utilizing 18s rdna to identify sarcocystis species in malaysian cattle and to determine their genetic variants. in this study, only sarcocystis cruzi was detected in malaysian cattle. the intra-species s. cruzi phylogenetic tree analysis and principal coordinate analy ... | 2015 | 26679818 |
| molecular characterisation of sarcocystis bovifelis, sarcocystis bovini n. sp., sarcocystis hirsuta and sarcocystis cruzi from cattle (bos taurus) and sarcocystis sinensis from water buffaloes (bubalus bubalis). | about 200 individual sarcocysts were excised from 12 samples of cattle beef from five countries (argentina, brazil, germany, new zealand, uruguay) and tentatively identified to species or cyst type on the basis of their size and shape and cyst wall morphology. genomic dna was extracted from 147 of these sarcocysts and used initially for pcr amplification and sequencing of the partial mitochondrial cytochrome c oxidase subunit i gene (cox1) in order to identify the sarcocysts to species and/or se ... | 2016 | 26677095 |
| sarcocystis heydorni, n. sp. (apicomplexa: sarcocystidae) with cattle (bos taurus) and human (homo sapiens) cycle. | cattle (bos taurus) are intermediate hosts for four species of sarcocystis, namely sarcocystis cruzi, sarcocystis hirsuta, sarcocystis hominis, and sarcocystis rommeli. of these four species, mature sarcocysts of s. cruzi are thin-walled (<1 μm), whereas s. hirsuta, s. hominis, and s. rommeli have thick walls (4 μm or more). here, we describe a new species of sarcocystis with thin-walled sarcocysts in cattle. two newborn calves were fed with sporocysts from the feces of a human volunteer who had ... | 2015 | 26243573 |
| prevalence and histopathological finding of thin-walled and thick-walled sarcocysts in slaughtered cattle of karaj abattoir, iran. | sarcocystosis is a zoonotic disease caused by sarcocystis spp. with obligatory two host life cycle generally alternating between an herbivorous intermediate host and a carnivorous definitive host. some species of this coccidian parasite can cause considerable morbidity and mortality in cattle. the present study was set to investigate the prevalence of sarcocystis spp. and type of cyst wall in slaughtered cattle of karaj abattoir, iran. for this purpose 125 cattle (88 males and 37 females) were i ... | 2015 | 26064016 |
| sarcocystis cruzi infection in wood bison (bison bison athabascae). | endangered wood bison (bison bison athabascae) is the largest terrestrial mammal in the american continent. animal health is an important issue in their conservation, and sarcocystis cruzi may be a cause of clinical disease in bovidae. hearts of eight wood bison from alaska, usa were examined for sarcocysts by histology, transmission electron microscopy, pepsin digestion, and molecularly. sarcocystis bradyzoites were found in pepsin digests of all eight and sarcocysts were found in histologic se ... | 2015 | 25868849 |
| microscopical and serological studies on sarcocystis infection with first report of s. cruzi in buffaloes (bubalus bubalis) in assiut, egypt. | this study was performed for the purpose of investigating the prevalence and the species composition of sarcocystis spp. in buffaloes in assiut province, egypt. macroscopically we reported the infection of buffaloes with sarcocystis fusiformis, while microscopically three sarcocystis species (sarcocystis cruzi, sarcocystis levinei and sarcocystis hominis) cysts were recognized, and were differentiated by their morphological features using both histopathological sections and electron microscope s ... | 2014 | 25320487 |
| sarcocystis sinensis is the most prevalent thick-walled sarcocystis species in beef on sale for consumers in germany. | bovines are intermediate hosts of sarcocystis cruzi, sarcocystis hirsuta, and sarcocystis hominis, which use canids, felids, or primates as definitive hosts, respectively. cattle represent also intermediate hosts of sarcocystis sinensis, but the definitive hosts of this parasite are not yet known. sarcocystosis in cattle is frequently asymptomatic. the infection is characterized by the presence of thin-walled (s. cruzi) or thick-walled muscle cysts or sarcocysts (s. hominis, s. sinensis, and s. ... | 2014 | 24700022 |
| sarcocystis cruzi: first molecular identification from cattle in iran. | sarcocystis is a genus of cyst-forming parasites infecting both animals and human. this study aimed to isolate coccidian tissue cysts from muscle of infected animals by a simple method in addition to molecular identification of sarcocystis cruzi from the samples. the samples were obtained from commercial source in babol, northern iran. five grams of calf muscle was cut into 2-3 pieces in 30 ml of phosphate-buffered saline containing 0.1% tween 80 and homogenized with ika t25, digital ultra-turra ... | 2013 | 24551802 |
| high prevalence of muscular sarcocystosis in cattle and water buffaloes from selangor, malaysia. | the prevalence of sarcocystosis in cattle and water buffaloes from peninsular malaysia was investigated in abattoirs in selangor state, february, 2011, to march, 2012. fresh muscle samples were collected from the tongue, heart, oesophagus, diaphragm and skeletal muscles of 102 cattle and 18 water buffaloes. each sample was initially screened by light microscopy and then fixed for further histopathological analysis. out of 120 animals examined, 49 (40.8%) harboured the microscopic type of sarcocy ... | 2013 | 24522140 |
| development of a multiplex real time pcr to differentiate sarcocystis spp. affecting cattle. | cattle are intermediate hosts of sarcocystis cruzi, sarcocystis hirsuta and sarcocystis hominis which use canids, felids or primates as definitive hosts (dh), respectively, and in addition of sarcocystis sinensis from which the dh is unknown. the aims of the present study were to develop and optimize a multiplex real time pcr for a sensitive and specific differentiation of sarcocystis spp. affecting cattle and to estimate the prevalence of sarcocystis spp. in argentinean cattle. the 18s rrna gen ... | 2013 | 23680541 |