| a surface glycoprotein of trypanosoma lewisi binds immunoglobulin g from the serum of uninfected rats. | detergent extracts of whole trypanosoma lewisi were fractionated by polyacrylamide gel electrophoresis and transferred to nitrocellulose for immunoblotting analysis. antibody probes to rat immunoglobulin g (igg) and igm detected intact gamma chains, mu chains, and light chains in extracts. the amount of immunoglobulin detected increased as the infection progressed. transfers were also incubated in serum from conventionally reared (crs), specific-pathogen-free (spfs), or germ-free rats before bei ... | 1986 | 3525414 |
| flow cytometric analysis of immunoglobulin and complement component c3 on the surface of trypanosoma lewisi. | we have reinvestigated whether surface immunoglobulin (sig) on trypanosoma lewisi is antibody directed toward parasite antigen by using flow cytometry to analyze parasites stained with fluoresceinated f(ab')2 fragments of antibodies to rat igg and igm. we have confirmed that igg antibody to the parasites is present both in the serum of rats and on the surface of parasites between the fourth and twentieth days of infection, that the amount of sig per cell increases as the infection progresses, an ... | 1986 | 3525820 |
| elicitation of the reproduction-inhibiting antibody ablastin by serum exoantigens of trypanosoma lewisi. | serum exoantigens of trypanosoma lewisi were collected 5 days after infection from immunocompetent (untreated) rats and rats immunosuppressed by treatment with either hydrocortisone acetate or dexamethasone. normal rats were then immunized with pooled, whole exoantigen-containing serum from 1 of these 3 sources plus alum as an adjuvant, and the immune sera produced were tested individually. all contained agglutinating (trypanocidal) antibodies to both antigenic variants of t. lewisi, but only ab ... | 1986 | 3546658 |
| isoenzyme characterization of trypanosomes of the subgenus herpetosoma. | isoenzyme analysis was used to characterize 6 species of trypanosomes of the subgenus herpetosoma using 13 different enzyme systems. the species studied were trypanosoma lewisi, t. musculi, t. grosi, t. microti, t. evotomys and t. nabiasi which cannot be distinguished on morphological grounds. extracts for thin-layer starch-gel electrophoresis were prepared from cultures of insect forms in either schneider's drosophila or grace's insect tissue culture media with foetal calf serum or a nutrient a ... | 1987 | 3547258 |
| the specific anti-parasite immune responses of germ-free and conventional rats infected with trypanosoma lewisi. | to test the hypothesis that the rapid immune response of rats to trypanosoma lewisi is elicited by prior exposure to cross-reacting environmental antigens, the early immune response to infection with this nonpathogenic protozoan was studied in germ-free and conventional rats. in germ-free rats, initial levels of both igg and igm were significantly lower than those of conventional rats. after infection, the germ-free rats made more immunoglobulins of both classes, and made them more quickly, than ... | 1987 | 3553515 |
| trypanosoma lewisi: restriction of alternative complement pathway c3/c5 convertase activity. | the rat parasite trypanosoma lewisi was incubated in vitro with rat or human serum, washed, and extracted in detergent. extracts were fractionated by electrophoresis in denaturing gels, transferred to nitrocellulose, allowed to renature, then immunoblotted with polyclonal antibodies to rat complement component c3 and human complement components c3, c5, and factor b. molecules that reacted with these antibodies were detected in the extracts. fragments of rat c3 were detected in extracts of parasi ... | 1987 | 3556206 |
| immunization of rats against trypanosoma lewisi infection. | | 1985 | 3912436 |
| trypanolytic activity, agglutinins, and opsonins in sera from animals refractory to trypanosoma lewisi. | an examination of sera from a range of animals refractory to trypanosoma lewisi showed that some contain trypanolytic activity and agglutinins for this trypanosome. trypanolytic activity was demonstrated in bovine, sheep, and rabbit serum. these sera were shown to also contain agglutinins. the trypanolytic activity in all three sera was abolished by heat inactivation at 56 degrees c for 30 min. studies with bovine serum showed that the lytic activity was totally inhibited by the addition of edta ... | 1985 | 3926649 |
| monoclonal antibodies to metacyclic stage antigens of trypanosoma cruzi. | hybridoma cell lines secreting monoclonal antibodies against the tulahuén strain of trypanosoma cruzi were produced by the fusion of sp2/o-ag 14 myeloma cells with spleen cells from mice immunized with irradiated metacyclic trypomastigotes. twenty of the monoclonals synthesized by the hybridomas were identified as igm, 2 as igg1, 10 as igg2a, 3 as igg2b, 4 as igg3, 1 as ige, and 1 as iga. twenty-three of these antibodies had kappa light chains and 18 showed delta chains. twelve of the monoclonal ... | 1985 | 3927760 |
| serologic characterization of the somatic antigens of forms of trypanosoma lewisi from the bloodstream and cultures. | | 1971 | 4107191 |
| the effect of some amino acids on carbohydrate uptake by trypanosoma lewisi. | | 1974 | 4156217 |
| survival and growth pattern of trypanosoma lewisi in two heterologous hosts: albino mouse and guinea-pig. | | 1972 | 4587804 |
| preliminary study of a trypanosoma lewisi-like parasite of rats in chiang mai, thailand. | | 1973 | 4588621 |
| mouse serum as an environment for the growth of trypanosoma lewisi. | | 1968 | 4172378 |
| [interferon-inducing capability of trypanosoma lewisi]. | | 1973 | 4593361 |
| chromatographic separation of a rat serum growth factor required by trypanosoma lewisi. | | 1969 | 4180860 |
| comparative studies on the persistence of trypanosoma (herpetosoma) musculi and t.(h.) lewisi in immune hosts. | | 1973 | 4593644 |
| physiological and antigenic changes in trypanosoma lewisi mediated by epinephrine. | | 1973 | 4596409 |
| influence of methods of preparation on the infectivity, agglutination, activity, and ultrastructure of bloodstream trypanosomes. | | 1974 | 4206929 |
| lack of protection against trypanosoma cruzi by multiple doses of t. lewisi culture forms. a discussion on some strains of "lewisi". | | 1974 | 4207915 |
| interaction of nutrition and infection: trypanosoma lewisi, folic acid levels in sera and tissues of normal and folic acid-deficient rats. | | 1974 | 4215256 |
| respiratory activity of trypanosoma lewisi during several phases of infection in the rat. | | 1968 | 4236168 |
| comparison of the effects of neonatal thymectomy on plasmodium berghei, trypanosoma lewisi and trypanosoma cruzi infection in the albino rat. | | 1974 | 4611081 |
| letter: trypanosomiasis in rats with trichinosis. | | 1974 | 4617351 |
| trypanosoma lewisi: creatine phosphokinase, ornithine carbamyl transferase, atpases, pi, and glucose levels in the rat host. | | 1968 | 4236169 |
| inhibition of reproduction in trypanosoma lewisi by ouabain. | | 1972 | 4260966 |
| the effects of adrenalectomy and splenectomy on trypanosoma lewisi infection in white rats. | | 1966 | 4288989 |
| the lipid metabolism of blood and culture forms of trypanosoma lewisi and trypanosoma rhodesiense. | | 1971 | 4330390 |
| trypanosoma lewisi: immune spleen cell transfer in rats. | | 1972 | 4559020 |
| trypanosoma lewisi: in vitro behavior of rat spleen cells. | | 1971 | 4944992 |
| trypanosome sterols and their metabolic origins. | | 1972 | 4561635 |
| electron microscope investigations of the structure of some specimens of the trypanosomidae family in connection with their ability to occur in the dyskinetoplastic state. 1. trypanosoma. | | 1972 | 4563776 |
| [protection induced in albino rats by the non-hydrosoluble fraction of trypanosoma lewisi against infection by the homologous strain]. | | 1971 | 4949103 |
| trypanosoma lewisi: termination of pregnancy in the infected rat. | | 1973 | 4570981 |
| trypanosoma lewisi: cell populations and antibody formation in riboflavin deficient rats. | | 1973 | 4574318 |
| [analysis and preparation of antigens from trypanosoma lewisi]. | | 1969 | 4987192 |
| [reaction of the mouse to serotherapy and infection with trypanosoma lewisi]. | | 1971 | 5002625 |
| the effect of the immunosuppressive agent cyclophosphamide on igg levels and parasite number in experimental trypanosomiasis. | | 1971 | 5003594 |
| transport of nucleosides in trypanosoma lewisi. | | 1973 | 4575538 |
| trypanosoma lewisi: duration of immune state in rats. | | 1972 | 4557723 |
| species-characteristic isoenzymes of two aminotransferases in trypanosomes. | | 1973 | 4578888 |
| interaction of nutrition and infection: effect of vitamin b12 deficiency on resistance to trypanosoma lewisi. | a metabolic imbalance technique was employed to study vitamin b(12) deficiency in rats infected with trypanosoma lewisi. throughout the observational period, animals on the deficient diet had lowered serum vitamin b(12) levels compared with complete and pair-fed animals. the decline in the level of the vitamin, ten days after the initiation of the experiment, continued progressively until the termination of the study. body weight gains and food consumption in rats on complete, vitamin b(12)-defi ... | 1985 | 3889354 |
| effect of some antibiotics and chemotherapeutics on trypanosoma lewisi infection in albino rats. | | 1985 | 3902989 |
| [identification of a laboratory strain hitherto believed to be trypanosoma lewisi (author's transl)]. | | 1973 | 4582780 |
| trypanosoma lewisi: electron microscopy of the infected spleen. | | 1973 | 4583054 |
| trypanosome identification by electrophoresis of soluble enzymes. | | 1973 | 4583509 |
| trypanosoma lewisi: oxygen uptake of rat liver mitochondria. | | 1973 | 4589787 |
| the relative activities of alanine and aspartate aminotransferases in bloodstream trypanosomes. | | 1973 | 4593642 |
| ultrastructure of trypanosoma lewisi, localization and alterations in rat liver. | | 1973 | 4599886 |
| isoenzymic differences between culture forms of trypanosoma rangeli, t. cruzi, and t. lewisi. | | 1974 | 4608715 |
| mechanical transmission of trypanosoma lewisi (kinetoplastida: trypanosomidae) by rhodnius prolixus (hemiptera: reduviidae). | | 1968 | 5699976 |
| growth and immunologic studies on the culture forms of trypanosoma lewisi. | | 1968 | 5703435 |
| trypanosoma lewisi: characteristics of an antigen which induced ablastic antibody. | | 1974 | 4609788 |
| trypanosoma lewisi: ultrastructural changes in rat liver. | | 1974 | 4611080 |
| trypanosoma lewisi: production of exoantigens during infection in the rat. | | 1972 | 4626124 |
| [protection of mice with trypanosoma lewisi antigen and its fractions against experimental trypanosoma cruzi infection]. | | 1972 | 4630386 |
| carnitine acetyltransferase activity in trypanosoma lewisi. | | 1971 | 4941537 |
| [some data on zooflagellate ribosomes]. | | 1971 | 4942544 |
| [on the host specificity of trypanosoma lewisi infection]. | | 1968 | 5719578 |
| the effects of antilymphocytic serum (als) on trypanosoma lewisi infection. | | 1971 | 4943629 |
| studies on the prevelence and effect of trypanosoma lewisi infection in finnish rats. | | 1971 | 4945234 |
| [experimental infection in mastomys natalensis (smith 1834) with trypanosoma species of the lewisi-congolense and brucei-evansi groups]. | | 1971 | 4946451 |
| further studies on antigenic changes in trypanosoma lewisi. | | 1968 | 4975999 |
| the attachment of trypanosoma lewisi in the rectum of its vector flea nosopsyllus fasciatus. | | 1969 | 5789066 |
| comparative immunological studies on somatic antigens and secretion-excretion products of blood stream and culture forms of trypanosoma lewisi. i. protective immunity and the agglutinin response. | | 1969 | 5795103 |
| [trypanosoma cruzi infection in mice following their sensitization with trypanosoma lewisi]. | | 1969 | 4976979 |
| [comparative studies of the desoxythymidylate synthesis in plasmodium chabaudi, trypanosoma gambiense and trypanosoma lewisi]. | | 1970 | 5275559 |
| trypanosoma lewisi infection in the rat: effect of adenine. | the reproductive activity of trypanosoma lewisi was strikingly enhanced and sometimes prolonged for 2 days when sublethal doses of adenine were given to rats. the drug was effective when given for 5 or 7 days in the diet and/or intraperitoneally, starting one or two days before infection through two days after infection. peak parasitemia was also enhanced. in terms of host immunity, daily sublethal doses of adenine given to rats within two days before or after infection with t. lewisi significan ... | 1971 | 5276290 |
| cultivation of trypanosoma lewisi in a dialysate medium. i. amino acid alterations during growth. | | 1969 | 5347604 |
| intracellular stages of trypanosoma lewisi in fleas and attempts to find such stages in other trypanosome species. | | 1969 | 5357319 |
| [fractional makeup of the lipids in cultures of trypanosoma lewisi i trypanosoma cruzi]. | | 1972 | 4580549 |
| the lipid composition of blood and culture forms of trypanosoma lewisi and trypanosoma rhodesiense compared with that of their environment. | | 1970 | 5440923 |
| host serum malic dehydrogenase activity associated with trypanosoma lewisi infection. | | 1967 | 6027419 |
| effect of holothurin on trypanosoma lewisi infections in rats. | | 1970 | 5449233 |
| kinetoplast deoxyribonucleic acid of the hemoflagellate trypanosoma lewisi. | cesium chloride centrifugation of dna extracted from cells of blood strain trypanosoma lewisi revealed a main band, rho = 1.707, a light satellite, rho = 1.699, and a heavy satellite, rho = 1.721. culture strain t. lewisi dna comprised only a main band, rho = 1.711, and a light satellite, rho = 1.699. dna isolated from dnase-treated kinetoplast fractions of both the blood and culture strains consisted of only the light satellite dna. electron microscope examination of rotary shadowed preparation ... | 1970 | 5497546 |
| trypanosoma lewisi: physiologic adaptation of wild isolate to albino rats evidenced by cell populations and respiratory activity. | | 1971 | 5556286 |
| trypanosoma lewisi infections in normal rats and in rats treated with dexamethasone. | | 1968 | 5643477 |
| fine structure and replication of the kinetoplast of trypanosoma lewisi. | the kinetoplastic dna of trypanosoma lewisi is described as a filamentous body lying within a mitochondrion, with the filaments oriented parallel to the long axis of the cell. the manner of fixation, the replicative state, and perhaps the physiological state of the cell, may result in slight morphological differences among such bodies. the kinetoplastic dna replicates to form "left" and "right" rather than "upper" and "lower" members, and both the kinetoplast and nucleus incorporate radiothymidi ... | 1968 | 5677967 |
| [studies on the experimental infection with trypanosoma lewisi in mice]. | | 1968 | 5707633 |
| the effects of pantothenic acid, pantoyltaurine, salicylate and related compounds on the in vitro and in vivo growth of trypanosoma lewisi. | | 1968 | 5710274 |
| [behavior of the blood mucoproteins in rats infected with trypanosoma lewisi]. | | 1968 | 5715925 |
| the effect of splenectomy on the production of ablastin and the termination of the parasitaemia in rats infected with trypanosoma lewisi. | this study investigated the effect of splenectomy on the production of ablastin and the elimination of trypanosoma lewisi from the circulation of infected porton rats. rats were splenectomized or sham-splenectomized either before or at various stages during the infection and the course of the parasitaemia compared with untreated control animals. ablastin titres in the serum were measured by a sensitive in vitro assay and were found to be the same in all the rats, regardless of the nature or timi ... | 1983 | 6349598 |
| [behavior of serum copper in rats during experimental infection with trypanosoma lewisi]. | | 1968 | 5715931 |
| [behavior of iron in the blood in experimental infection with trypanosoma lewisi]. | | 1968 | 5715932 |
| comparison of mitochondrial enzymes between young and adult forms of trypanosoma lewisi. | | 1968 | 5719060 |
| the fine-structure of the epimastigote forms of trypanosoma lewisi in the rectum of the flea, nosopsyllus fasciatus. | | 1969 | 5769498 |
| carbohydrate transport in trypanosoma lewisi. | | 1969 | 5778271 |
| comparative studies on blood stream and culture forms of trypanosoma lewisi. i. oxygen utilization, carbon dioxide production and glucose uptake. | | 1969 | 5788607 |
| [immunological relations between trypanosoma cruzi and trypanosoma lewisi]. | | 1969 | 5788822 |
| development of trypanosoma lewisi in experimental chimaeras. | | 1969 | 5806200 |
| serum transaminases and aldolase in rats inoculated with trypanosoma lewisi. | | 1966 | 5933980 |
| oxygen uptake by trypanosoma lewisi-complex cells. ii. r and l isolates compared. | | 1966 | 5940094 |
| a trypanosoma lewisi-like organism from the rabbit, sylvilagus floridanus, in virginia. | | 1966 | 5942537 |
| oxygen uptake by trypanosoma lewisi complex cells. 3. c isolate. | | 1966 | 5943918 |
| intracellular stages of trypanosoma lewisi in fleas and attempts to find such stages in other trypanosome species. | | 1969 | 5374709 |
| comparative studies on blood-stream and culture forms of trypanosoma lewisi. ii. biochemical composition. | | 1970 | 5435731 |
| two identical symmetrical regions in the minicircle structure of trypanosoma lewisi kinetoplast dna. | two minicircles of trypanosoma lewisi kinetoplast dna, linearized by different restriction enzymes, have been cloned in m13 derivative vectors. their nucleotide sequences share a dna fragment which accounts for 10% of the minicircle total length. this highly conserved region (constant region) is present twice within the same minicircle in a direct orientation and in an almost symmetrical location. comparison of the t. lewisi constant region with those of t. brucei and t. equiperdum shows identic ... | 1984 | 6096711 |
| trypanostatic activity of rat igg purified from the surface coat of trypanosoma lewisi. | host igg is a component of the surface coat of trypanosoma lewisi; it is specifically acquired during infection in the rat, concomitant with a rise in titer of trypanostatic (ablastic) activity of host serum. host igg was eluted from trypomastigotes at 7 to 9 days postinfection with a high salt-low ph buffer. surface coats and trypanosome ultrastructure were not notably altered by the elution procedure, as determined by electron microscopy. rat igg was removed and purified from the trypanosome e ... | 1982 | 6182278 |
| correlation of oligomycin-sensitive atpase activity in trypanosomes with their content of an antigen to primary biliary cirrhosis. | the amounts of an antigen to primary biliary cirrhosis (pbc) which occur in subcellular fractions of trypanosoma rhodesiense and t. lewisi correlate positively with the oligomycin-sensitive (os) atpase activity of these fractions. this result is consistent with the mitochondrial atpase association of the antigen in mammalian and other cells. higher levels of os-atpase and of pbc antigen in t. lewisi accord with a more extensive mitochondrial development in this species. | 1984 | 6239698 |
| molecular cloning of the "minor" component of the kinetoplast dna of trypanosoma lewisi. | kinetoplast dna was cloned in the pbr322 plasmid using the bamhi site. in situ hybridization of iodinated recombinant dna showed hybridization grains only in the kinetoplast region. the recombinant dna was analyzed with restriction enzymes and its base ratio was determined by analytical cscl density gradient. | 1980 | 6257401 |