| an electrophysiological investigation of skeletal muscle in human chronic chagas' disease. | an electrophysiological study has been made of the thenar, hypothenar, soleus and extensor digitorum brevis muscles and their inervation in 90 patients with chronic chagas' disease. some of them showed a reduced number of functional motor units with increased size of many of the surviving units. no decremental muscle response was found to repetitive nerve stimulation. motor and sensory conduction velocities as well as motor terminal latencies were on the normal range. these findings suggested th ... | 1978 | 109066 |
| suppressor cells present in the spleens of trypanosoma cruzi-infected mice. | infection with trypanosoma cruzi decreases the ability of spleen cells from mice to respond to either t cell, concanavalin a (con a), or b cell, lipopolysaccharide (lps), mitogens. the effect of infection on the mitogenic response depends on the elapsed time between the day of infection and the time of mitogen presentation. responses early in infection are normal, whereas later responses to either mitogen are depressed. spleen cells from late trypanosome-infected mice inhibit the ability of norm ... | 1979 | 109508 |
| chemotherapy with ethidium bromide-dna complex in established chagas' disease. | two different strains of mice (akr and nmri-ivic) were inoculated intraperitoneally with the virulent y strain of trypanosoma cruzi, and then treated with the lysosomotropic ethidium bromide-dna complex, according to several different treatment schedules. when animals were treated 48 hours after intraperitoneal inoculation with three intraperitoneal doses of eb-dna no parasitemia was detected, even after 11 weeks, confirming previous results. however, when infection was allowed to become fully e ... | 1979 | 110160 |
| prevalence of parasitemia and seroreactivity to trypanosoma cruzi in a rural population of northeast brazil. | age-specific prevalence rates of parasitemia and seroreactivity to trypanosoma cruzi were determined in a rural area endemic for chagas' disease in northeast brazil. parasitemia was detected by blood cultures and xenodiagnosis, and serum antibodies to the parasite were measured by the complement fixation (cf) and indirect immunofluorescence (ifa) tests. of the 116 persons examined, 39 (33.7%) had antibodies and 23 (19.8%) had parasitemia. ninety-six percent of parasitemic individuals were seropo ... | 1979 | 110161 |
| antibody-dependent cytolysis of trypanosoma cruzi by human polymorphonuclear leukocytes. | | 1979 | 110466 |
| thymus-dependent control of host defense mechanisms against trypanosoma cruzi infection. | congenitally athymic homozygous (nu/nu) mice were shown to be significantly more susceptible to trypanosoma cruzi infection than their thymus-bearing heterozygous (nu/+) littermates, as measured by increased parasitemia, mortality rate, and shortened survival time. in addition, transplantation of neonatal thymus into athymic mice reestablished normal levels of resistance to t. cruzi, i.e., comparable to those of normal littermates. these results constitute conclusive evidence that host defense m ... | 1979 | 110686 |
| american trypanosomiasis: chagas' disease. | | 1979 | 110719 |
| screening and structure-activity relationships of synthetic juvenile hormone analogues for panstrongylus megistus, a primary vector of chagas' disease in brazil. | | 1978 | 110859 |
| [wild reservoirs and vectors of trypanosoma cruzi. lxxi: comparative study on the importance of the port of entry of metacyclic trypomastigotes in experimental t. cruzi infection]. | | 1979 | 111308 |
| [wild reservoirs and vectors of trypanosoma cruzi. lxxiii: resistance against wild strains of t. cruzi in mice inoculated with herpetomas samuelpessoai]. | | 1979 | 111309 |
| [wild reservoirs and vectors of trypanosoma cruzi. lxxii: behavior of the pf strain of t. cruzi in mice inoculated with flagellates obtained from triatominal infected by artificial xenodiagnosis or by inoculation into the coelomic cavity]. | | 1979 | 111310 |
| the protective effect of non-immunologic granulomatous inflammation in trypanosoma cruzi infection of mice. | | 1979 | 111311 |
| [early immunological aspects in rats infected by trypanosoma cruzi. ii. soluble antigen circulation and the modifications of serum complement from animals in sucessive days of the infection (author's transl)]. | a group of rats was infected by the y strain of t. cruzi and three animals were bled daily from the 4th to the 13th days. the sera obtained each day, after being mixed, were used for dosing serum complement and for acting as antigens in the cft with a chagas' disease serum. the amount of circulating antigen increased gradually, reached a maximum in the 5th and 6th days and then decreased up to the 10th day; thereafter it increased attaining a new maximum in the 11th day and again decreased up to ... | 1979 | 111312 |
| [prevalence of trypanosoma cruzi infection, in 1975, in 2 blood banks of londrina, paraná, brazil]. | | 1978 | 111340 |
| [focal point investigation, 1 activity of the campaign of control of transmitters of american trypanosomiasis]. | | 1978 | 111342 |
| in vivo and in vitro interaction of trypanosoma cruzi with mouse macrophages and heart muscle cells--film presentation. | | 1979 | 111486 |
| improvements in routine xenodiagnosis with first instar dipetalogaster maximus (uhler 1894) (triatominae). | first instar dipetalogaster maximus proved as effective as third instar triatoma infestans in detecting circulating trypanosoma cruzi in patients with chronic infections examined in a routine xenodiagnostic laboratory. since rearing costs are diminished, d. maximus has now replaced t. infestans as the xenodiagnostic agent in our laboratory. however, further work is needed to define the optimal conditions necessary when using this species in xenodiagnosis. | 1979 | 111570 |
| circulating antibodies to peripheral nerve in american trypanosomiasis (chagas' disease). | an antibody reacting with schwann sheaths of myelinated somatic and unmyelinated autonomic peripheral nerve was found in sixty-one out of seventy-one chronic, and nine out of ten acute, chagas' disease sera. indirect immunofluorescence (ifl) was carried out on rat, mouse and human somatic nerves and rat sympathetic nerves with initial serum dilutions of 1 : 10, and the staining reached a final titre of 1 : 320 in some cases. the antibodies fixed complement and were absorbed out by lyophilized ep ... | 1979 | 111884 |
| studies of the domestic ecology of triatoma infestans by means of house demolition. | | 1979 | 112663 |
| [action of aspergillus sp. on trypanosoma cruzi in vitro]. | | 1979 | 112665 |
| chagas's disease in the amazon basin: 1. trypanosoma cruzi infections in silvatic mammals, triatomine bugs and man in the state of pará, north brazil. | a total of 1,197 wild animals from pará state north brazil, were examined for haematozoa. trypanosoma cruzi-like parasites were found in 13 different species, and were particularly common in a variety of marsupials (didelphidae), porcupines (coendou spp.), armadillos (dasypus novemcinctus) and coatimundis(nasua nasua). three human infections are reported, making a total of seven autochthonous cases of chagas's disease from pará since the first were described in 1969. a serological survey of 5,31 ... | 1979 | 112730 |
| enhancement of host resistance against trypanosoma cruzi infection by the immunoregulatory agent muramyl dipeptide. | n-acetylmuramyl-l-alanyl-d-isoglutamine (muramyl dipeptide [mdp]) enhanced resistance against trypanosoma cruzi infection in mice. this effect was evidenced by significant reductions in both parasitemias and mortality rates and increased survival time in mdp-treated animals compared with untreated infected mice. mdp effectively augmented host resistance when administered in any one of the following ways: (i) continuous subcutaneous release from an osmotic minipump for a 7-day period starting 2 d ... | 1979 | 113344 |
| antibody-dependent cellular cytotoxicity of trypanosoma cruzi: characterization of the effector cell from normal human blood. | the antibody-dependent cellular cytotoxicity activity of normal human blood cells against epimastigotes of trypanosoma cruzi was measured by the release of incorporated [3h]uridine. sera from patients with chronic chagas' disease were used to sensitize the parasites to the lytic activity of the effector cells. different steps of peripheral blood cell purification were employed, and different cell subpopulations were tested as effectors in the system. the main cytotoxic activity was detected in t ... | 1979 | 113345 |
| [circulating immune complexes in experimental chagas' disease. identification of parasitic antigens in the complexes]. | | 1979 | 113861 |
| [chagas polyneuropathies]. | the results of a research realized in a small community in the countryside of the state of bahia, brazil, where there is high frequency of infection by trypanosoma cruzi. are reported. by evaluation the neurological clinical examination of 99 individuals it was observed that the most frequent finding on those 50 patients with positive sorology there was a diminution of deep reflexes. additionally, it was found that among the 20 patients in whom more than one altered deep reflex was present, in 1 ... | 1979 | 115447 |
| adoptive transfer of protection against trypanosoma cruzi with lymphocytes and macrophages. | female c57bl/6j mice were infected with trypanosoma cruzi and subsequently given macrophages or lymphocytes from syngeneic donors which had recovered from the acute infection. mice which received immune peritoneal macrophages, splenic lymphocytes, or lymph node lymphocytes developed lower mean parasitemias and cumulative mortalities than did recipients of nonimmune cells. neither peritoneal lymphocytes nor splenic macrophages were protective, however. these studies indicate that splenic and lymp ... | 1979 | 115790 |
| urinary metabolites of 3a,4,5,6,7,7a-hexahydro-3-(1-methyl-5-nitro-1h-imidazol-2-yl)-1,2-benzisoxazole in the dog. | the antiprotozoal drug 3a,4,5,6,7,7a-hexahydro-3-(1-methyl-5-nitro-1h-imidazol-2-yl)-1,2-benzisoxazole (i), which exhibits activity against trypanosomiasis, is also antibacterial in vivo. since the urine from a dog dosed with i showed a broader spectrum of antibacterial activity than i itself, metabolites from this urine were isolated and partially characterized. the metabolites were mono- and dihydroxy-substituted species with the hydroxyl groups on carbons 4--7 of the hexahydrobenzisoxazole ri ... | 1979 | 115987 |
| [trypanosoma cruzi: activity of mouse immune sera on the tyrpomastigote stage]. | | 1979 | 116105 |
| [pregnancy and trypanosoma cruzi parasitism]. | | 1979 | 116106 |
| protective immunisation of mice using cell surface glycoprotein from trypanosoma cruzi. | | 1979 | 116128 |
| antibody-dependent killing of bloodstream forms of trypanosoma cruzi by human peripheral blood leukocytes. | bloodstream forms of trypanosoma cruzi were found to be destroyed by human lymphoid cells, neutrophils, or eosinophils in the presence of specific antibodies. nonspecific immunoglobulins present in normal serum did not mediate the cytotoxic reaction. cells or antibody had no significant lytic effect on the parasite when tested separately and purified, non-activated human adherent cells were inactive antibody was present or not. | 1979 | 116558 |
| use of micro-elisa for quantitating antibody to trypanosoma cruzi and trypanosoma rangeli. | of 229 residents of a panamanian village where both trypanosoma cruzi and trypanosoma rangeli are endemic, 52% had antibody to one or both species by the enzyme-linked immunosorbent assay (elisa), 26% were positive by complement fixation, 17% were positive by direct agglutination, and 32% were positive on the basis of clinical impression. although the sensitivity of elisa makes it the procedure of choice for sero-epidemiologic studies, there does appear to be some serologic cross-reactivity betw ... | 1979 | 116559 |
| [etiologic therapy of chagas' disease]. | | 1979 | 116629 |
| membrane-bound antibodies to bloodstream trypanosoma cruzi in mice: strain differences in susceptibility to complement-mediated lysis. | the y, cl and other strains of trypanosoma cruzi display different morphological and immunological characteristics. such observations are here extended to the interaction of bloodstream forms of different strains of t. cruzi with components of the complement system. we demonstrate that the bloodstream forms of the y and b strains, but not those of the cl strain, are lysed by normal human serum. lysis is mediated by combined activities of the alternative and classical complement pathways. these a ... | 1979 | 116784 |
| trypanosoma cruzi: homocytotrophic antibody response in mice immunized with unrelated antigens. | | 1979 | 116864 |
| immunochemical study of the lysates of the epimastigote forms of trypanosoma cruzi (chagas, 1909). | | 1979 | 117521 |
| [ecological aspects of south american trypanosomiasis. xiv. persistence and domicilization potential of wild triatomid populations in an intensive agricultural and cattle breeding region]. | | 1979 | 117542 |
| [an autopsy case of chronic chagas' disease (author's transl)]. | | 1979 | 118226 |
| in vitro cellular immunity in chagas' disease. | in vitro delayed hypersensitivity was studied in patients with either the chronic or the indeterminate phase of chagas' disease. normal healthy individuals were used as controls. the leucocyte migration inhibition test was applied using an antigen an extract of culture forms of t. cruzi or an extract of normal rat heart. the results showed that patients with the chronic phase of the disease reacted significantly with both antigens, whereas most patients with the indeterminate phase did not react ... | 1979 | 118840 |
| genetic control of the mechanisms of resistance against experimental trypanosoma cruzi infection. i: role of the macrophages in resistant and suceptible strains of mice. | | 1979 | 119283 |
| carlos chagas (1879-1934): the discovery of trypanosoma cruzi and of american trypanosomiasis (foot-notes to the history of chagas's disease). | | 1979 | 119337 |
| dipetalogaster maximus (hemiptera, triatominae) for xenodiagnosis of patients with serologically detectable trypanosoma cruzi infection. | in patients serologically positive for trypanosoma cruzi infection the three bug species/instar combinations used in xenodiagnosis showed third-instar dipetalogaster maximus to be more efficient in detecting circulating trypanosomes than the first instar of the same species which, in turn, is more sensitive than third-instar triatoma infestans. the sensitivity of the pool technique of faecal examination compared with individual dissection was investigated. four pool examinations (the product of ... | 1979 | 119338 |
| experimental trypanosoma cruzi infection in rhesus monkeys 111. electrocardiographic and histopathological findings. | in five rhesus monkeys surviving 'peru strain' or 'strain 7' trypanosoma cruzi infection for six to eight years, positive xenodiagnosis results and high indirect fluorescent antibody titres (4096 - 65536) persisted until the animals were killed. abnormal electrocardiograph patterns in two monkeys (h and k) were possibly compatible with myocardial damage. histopathological changes attributable to t. cruzi infection were minor in four monkeys but severe in one (r). in this animal, infected with wh ... | 1979 | 119339 |
| attempts to produce megasyndrome in mice using stocks of trypanosoma cruzi associated with megaoesophagus in man. | two stocks of trypanosoma cruzi isolated from patients with advanced megaoesophagus produced megastomach in chronically infected mice. the mice showed evidence of stomach dilatation and a delay in intestinal transit time. these findings are discussed in the light of the hypothesis that regional variations in t. cruzi determines mega formation in man. | 1979 | 120044 |
| an outbreak of acute chagas's disease in the são francisco valley region of bahia, brazil: triatomine vectors and animal reservoirs of trypanosoma cruzi. | following reports of an unusually high incidence of acute chagas's disease and the appearance of large numbers of triatoma infestans in the southwestern region of the state of bahia, triatomine bugs (hemiptera: reduviidae) and domestic animals in one of the affected communities were surveyed and examined for infection with trypanosoma cruzi. triatoma infestans was prevalent in houses and was also found in peridomestic habitats. t. sordida and t. pseudomaculata occupied peridomestic and sylvatic ... | 1979 | 120045 |
| chagas' disease: trends in immunological research and prospects for immunoprophylaxis. | acute infection with trypanosoma cruzi usually subsides spontaneously but the mortality rate encountered in individuals with the chronic infection is high. much evidence has accumulated in the last five years that autoimmunity plays an important role in the pathogenesis of the myocarditis that is common in the chronic phase. a negative relationship has been observed between the demonstrable parasitaemia and the presence of severe cardiac lesions. this myocarditis is characterized by lymphocytic ... | 1979 | 120233 |
| immunization against experimental trypanosoma cruzi infection: inherent difficulties of a uniform comparative evaluation of antigenic preparations. | | 1979 | 120626 |
| the effect of lampit on trypanosoma cruzi in mice organs and in the bloodstream. | mice infected with bloodstream forms of trypanosoma cruzi were treated with an active nitrofuran compound (nifurtimox, lampit). determination of the number of intracellular forms of t. cruzi in the liver and the spleen of control and lampit-treated mice showed that the drug induced a decrease in the number of parasites inside the cells. a decrease in the number of bloodstream forms was also observed. ultrastructural observations showed that lampit induces several alterations in t. cruzi, the mos ... | 1979 | 120645 |
| immune response in human chagas' disease i. lymphocyte blastogenesis in chagasic patients without evidence of cardiomyopathy. | | 1979 | 120666 |
| protection against trypanosoma cruzi using fecal forms of homologous parasites from juvenilized or normal vectors of chagas' disease and by passive transfer of immune blood. | | 1977 | 120952 |
| [transfer of humoral immunity to trypanosoma cruzi induced by heat in mice]. | | 1979 | 120975 |
| report on a field collection of dipetalogaster maximus (hemiptera triatominae) (uhler, 1894). | | 1979 | 120977 |
| [reservoirs and wild vectors of trypanosoma cruzi. lxxiv. behavior of amastigotes and trypomastigotes of cultures of different t. cruzi strains incubated with normal human serum and inoculated into mice]. | | 1979 | 121615 |
| depression of blood monocytes chemotaxis in human chronic chagas disease. | | 1979 | 121632 |
| effects of parasitic infections in pregnant women. | this review focuses on the parasitic diseases which occur frequently in the tropics and which affect pregnant women. clinical disease of the mother during pregnancy, vertical transmission of parasites and transplacental passage of soluble parasitic antigens are discussed in relation to their immunopathological significance for the fetus. the incidences of congenital malaria, african trypanosomiasis and chagas' disease are reviewed, together with vertical transmission of filarial infection, invol ... | 1979 | 121975 |
| a comparison of challenge with trypanosoma cruzi blood-stream trypomastigotes and metacyclic trypomastigotes from rhodnius prolixus in mice immunized with killed antigens. | groups of cd-1 mice were immunized with vaccines prepared from freeze-thawed or ultra-sonicated epimastigotes, blood trypomastigotes, or "plasma antigen", of trypanosoma cruzi strains y, m1 and tulahuen. the mice were challenged by the injection of blood stream trypomastigotes obtained from mice, or of metacyclic trypomastigotes harvested from the rectum of rhodnius prolixus. both challenges induced virulent infections in control mice. blood-stream trypomastigotes killed mice more rapidly than t ... | 1979 | 122124 |
| [congenital chagas disease: anatomopathological findings in the placenta and umbilical cord]. | | 1979 | 122217 |
| [chagas disease: vector control activities in the state of são paulo, brazil]. | | 1979 | 122244 |
| [solid-phase radioimmunoassay for the study of the humoral response to trypanosoma cruzi]. | a solid-phase micro-radioimmunoassay (rie-s) test was adapted fore the study of the humoral immune response induced by trypanosoma cruzi infection. the solid phase, consisting of disposable polypropylene tubes, was sensitized by absorption of t. cruzi antigen. the procedure was performed by sequential adding to the solid-phase, the human serum under study and 131i anti-human immunoglobulin. then, the specific label binding was estimated. several factors which influence the test were studied. the ... | 1979 | 122384 |
| immunopathologic and morphologic studies of skeletal muscle in chagas' disease. | skeletal muscle biopsies from 21 individuals infected with trypanosoma cruzi were studied my means of immunofluorescence, ultrastructural immunochemical, light and electron microscopic, and histochemical procedures. in 12 cases, definite morphologic alterations were found. these alterations were coincident with the presence of circulating antibodies against the plasma membrane of striated muscle fibers and endothelial cells (evi antibodies). in almost all cases the lesions also presented auto ... | 1975 | 125546 |
| immunological studies in rockland mice infected with t. cruzi. development of antinuclear antibodies. | the existence of antinuclear antibodies (ana) and their relationship with anti-trypanosoma cruzi specific agglutinins was studied in rockland mice inoculated with 10(5), 10(4) or 10(3) epimastigotes and 2 x 10(5) trypomastigotes of t. cruzi. ana and anti-t. cruzi agglutinins were detected at the same time in groups of mice receiving 10(5) culture forms, while in those groups of mice receiving 10(4) parasites agglutinins were established earlier than ana which were present only at low titres. pos ... | 1975 | 131587 |
| [symposium on new approaches in research on american trypanosomiasis]. | | 1977 | 142492 |
| studies on the vesicular component of the auerbach's plexus and the substance p content of the mouse colon in the acute phase of the experimental trypanosoma cruzi infection. | a dramatic reduction in the total number of dense vesicles in auerbach's plexus of the mouse colon was observed during the acute phase of experimental american trypanosomiasis (chagas' disease). a significant decrease in substance p activity of the colon of inoculated animals was also measured. it is suggested that this decrease in substance p activity could be related to the reduction in the number of dense vesicles in auerbach's plexus. | 1977 | 145726 |
| trypanosoma cruzi: isolation and characterization of membrane and flagellar fractions. | | 1978 | 153234 |
| [domestic triatomines (reduvidae) and the trypanosomic infections in insects in el salvador]. | | 1979 | 154908 |
| adenosine triphosphatase activities in trypanosoma cruzi. | 1. subcellular fractions obtained from epimastigotes of trypanosoma cruzi, disrupted by three different procedures, contained in addition to the already known mg2+-activated adenosine triphosphatase (atpase; e.c.3.6.1.4), a ca2+-atpase activity. 2. the ca2+-atpase (a) was activated by low concentrations of cacl2 (apparent ka, 80 microm); (b) had a km for atp of 0.6 mm (at 1 mm cacl2, ph 8.0); (c) presented a broad ph curve (optimum 7.1-8.6); and (d) was insensitive to oligomycin concentrations w ... | 1978 | 162583 |
| solubilization and some properties of the mg2+-activated adenosine triphosphatase from trypanosoma cruzi. | 1. grinding of epimastigotes of trypanosoma cruzi with glass powder in a mortar yielded a mg2+-activated adenosine triphosphatase (atpase) preparation which was highly sensitive to oligomycin. 2. chloroform treatment of the particles resulted in the solubilization of an atpase which was (a) activated by mgcl2; (b) slightly inhibited by cacl2; (c) activated by sulphite and bisulphite; (d) had an optimum ph of 7.6; and (e) had a km for atp of 2.1 mm (in the presence of 4 mm mgcl2). 3. the solubili ... | 1978 | 162584 |
| isoenzyme patterns of cultured trypanosoma cruzi: changes after prolonged subculture. | 1. the isoenzyme patterns of four soluble enzymes in seven stocks of t. cruzi were determined by electrophoresis. according to their patterns they could be classified into four sets. 2. the isoenzyme patterns of two stocks were influenced by the number of subcultures. 3. five stocks from man are distinct from those derived from a silvatic reservoir. 4. since the isoenzyme patterns of a stock isolated from a patient with acute disease were similar to those of a silvatic reservoir, its recent intr ... | 1979 | 162589 |
| in vitro studies on the differential toxicity of metronidazole in protozoa and mammalian cells. | | 1975 | 164840 |
| immunization against experimental chagas' disease by using culture forms of trypanosoma cruzi killed with a solution of sodium perchlorate. | protection against infection with virulent blood (trypomastigote) forms of trypanosoma cruzi was accomplished in mice by immunization with culture (mainly epimastigote) forms killed by treatment with sodium perchlorate. sodium chloride, used instead of sodium perchlorate, with all other conditions kept the same, failed to kill all the organisms, indicating that the effects of the perchlorate anion were not simply ionic or osmotic, suggesting that they might be chaotropic. a single dose of the im ... | 1975 | 170200 |
| chemotherapeutically active nitro compounds. 4. 5-nitroimidazoles (part i). | more than 135 new 2-methyl-5-nitroimidazoles substituted in 1-position and 1-methyl-5-nitroimidazoles substituted in 2-position were investigated for their activity against various protozoan species, in particular entamoeba histolytica in the golden hamster, trichomonas fetus, trypanosoma brucei and t. cruzi in the nmri mouse. among the nitroimidazoles substituted in the 1-position only two preparations exhibited a similar effect as metronidazole preparations exhibited a similar effect as metron ... | 1977 | 203293 |
| lipid peroxidation and the generation of free radicals, superoxide anion, and hydrogen peroxide in beta-lapachone-treated trypanosoma cruzi epimastigotes. | | 1978 | 205176 |
| isolation of blood and intracellular forms of trypansoma cruzi from rats and other rodents and preliminary studies of their metabolism. | isolation of blood and intracellular forms of trypanosoma cruzi was made mainly from rats (90-110 g) which had received 580 rad of whole-body gamma-irradiation not more than 24 h before subcutaneous inoculation with 10(7) trypomastigotes of the sonya strain of t. cruzi. unirradiated chinchillas (250-350 g) were, however, used for some experiments. blood forms were isolated using a technique involving differential centrifugation to remove most of the erythrocytes and deae-cellulose chromatography ... | 1978 | 206867 |
| immunization of mice against trypanosoma cruzi: the effect of chemical treatment or immune serum on an epimastigote vaccine. | groups of long evans and cd-1 mice were immunized with vaccines prepared from epimastigotes of trypanosoma cruzi, strains y and tulahuen, modified by various chemical means, or by the addition of immune sera. freeze-thawed epimastigotes were used as a control antigen. freeze-thawed epimastigotes, with and without saponin as adjuvant, gave significant protection against challenge with homologous trypomastigotes. similar levels or protection were obtained in mice vaccinated with living epimastigot ... | 1978 | 209592 |
| trypanosoma cruzi: role of the immune response in the natural resistance of inbred strains of mice. | | 1978 | 210036 |
| ultrastructural alterations and peroxide formation induced by naphthoquinones in different stages of trypanosoma cruzi. | addition of beta-lapachone, an o-naphthoquinone with bactericidal, cytotoxic, and trypanocidal activities, to trypanosoma cruzi epimastigote and amastigote stages induced the release of o2- and h2o2 from the whole cells into the suspending medium. in the presence of reduced nicotinamide adenine dinucleotide as reductant beta-lapachone was also able to stimulate o2- and h2o2 production by homogenates of these stages. electron micrographs showed that in beta-lapachone-treated amastigotes and trypo ... | 1978 | 216177 |
| corynebacterium parvum as an adjuvant for trypanosoma cruzi epimastigote vaccines: a comparison with saponin and bordetella pertussis. | the effect was compared in cba mice of adding corynebacterium parvum, saponin, and bordetella pertussis to living or killed trypanosoma cruzi (y strain) epimastigote vaccines on the induction of protective immunity against subcutaneous (s.c.) challenge with blood trypomastigotes. the addition of c. parvum to a low dose of t. cruzi vaccine, which alone was non-protective, generated a greater degree of protection than did saponin or b. pertussis. c. parvum alone increased resistance to infection t ... | 1979 | 216967 |
| effect of beta-lapachone on superoxide anion and hydrogen peroxide production in trypanosoma cruzi. | addition of beta-lapachone, an o-naphthoquinone endowed with trypanocidal properties to respiring trypanosoma cruzi epimastigotes induced the release of o2- and h2o2 from the whole cells to the suspending medium. the same beta-lapachone concentration (4 micron) that released h2o2 at maximal rate completely inhibited t. cruzi growth in a liquid medium. the position isomer, alpha-lapachone, did not stimulate o2- and h2o2 release, and did not inhibit epimastigote growth. beta-lapachone was able to ... | 1978 | 217340 |
| conversion of dihydroorotate to orotate in parasitic protozoa. | the conversion of dihydroorotate to orotate, one of the key reactions in the de novo pyrimidine biosynthetic pathway, has been studied in a number of parasitic protozoa. enzyme activities capable of carrying out this reaction were detected in six members of the kinetoplastida (trypanosoma brucei, trypanosoma congolense, trypanosoma vivax, trypanosoma lewisi, trypanosoma cruzi, leishmania enriettii) and three members of the genus plasmodium (p. knowlesi, p. berghei, p. gallinaceum). the mechanism ... | 1979 | 217438 |
| oxidative metabolism in mammalian and culture forms of trypanosoma cruzi. | | 1979 | 229013 |
| [cytochromes in epimastigote form of trypanosoma cruzi]. | | 1979 | 231798 |
| generation of superoxide anion and hydrogen peroxide induced by nifurtimox in trypanosoma cruzi. | | 1979 | 232403 |
| [study of brazilian agricultural workers. a medico-socio-psychological study]. | the general state of health of native brazilian agricultural workers - a total of 750 people from 3 different plantations in the states of paraná and saso paulo - was examined. the main interest of this study war centred on infectious and parasitic diseases, nutritional conditions as well as social, intellectual factors. observations: apart from the high number of cases of helminthiasis, amounting to 80%, the general state of health of the examined subjects was found to be good - indeed better t ... | 1975 | 239549 |
| trypanosoma lewisi, t. acomys and t. cruzi: a method for their cultivation with mammalian tissue. | | 1977 | 320031 |
| inhibition of protein kinase activity from trypanosoma cruzi and trypanosoma gambiense by 3'-deoxyadenosine. | 3'-deoxydadenosine was found to be a potent inhibitor of nucleoside-stimulated protein kinase activity from culture forms of trypanosoma cruzi and bloodstream forms of trypanosoma gambiense. the type of inhibition by 3'-deoxyadenosine was competitive with respect to atp. the inhibition constants for 3'-deoxyadenosine were determined to be 0.11mm and 0.085mm for the enzyme from t. cruzi and t. gambiense, respectively. the apparent km value for atp was 0.2mm for both enzymes. 2'-deoxyadenosine was ... | 1977 | 320116 |
| feeding preferences of triatoma dimidiata maculipennis in yucatan, mexico. | to determine whether a low preference for human blood by triatoma dimidiata maculipennis stal, 1859, the only known vector of chagas' disease in yucatan, mexico, would account for the low prevalence of antibodies to trypanosoma cruzi in man in the area, the intestinal contents of 924 bugs were tested against antisera to blood antigens of chicken, opossum, dog, bat, monkey, pig, goat, rabbit, horse, human, rat, ox, cat, and armadillo. although the chicken was the primary host, man was fed upon fr ... | 1977 | 320892 |
| the effects of lampit (bayer 2502) on the interaction of trypanosoma cruzi with vertebrate cells in vitro. | the effects of 3-methyl-4-(5'-nitrofurfurylidene-amino)-tetrahydro-4h-1,4-thiazine-1,1-dioxide [lampit, bayer 2502] on the intracellular cycle of trypanosoma cruzi were studied with an in vitro steady-state culture system that permitted the continuous analysis of individual host cell-parasite interactions. lampit concentrations of 10(-4) and 10(-5) m significantly affected the ability of trypomastigotes to penetrate vertebrate cells. lampit concentrations above 10(-4) m were toxic to the host ce ... | 1977 | 320896 |
| trypanosoma cruzi: effect of ethidium bromide on growth, dyskinetoplasty, and respiration of the y and costa rica strains. | | 1977 | 321238 |
| biochemical strain characterization of trypanosoma cruzi by restriction endonuclease cleavage of kinetoplast-dna. | | 1977 | 321246 |
| effect of human monocytes and macrophages on trypanosoma cruzi. | studies were undertaken to determine whether trypanosoma cruzi can invade and multiply within human monocytes and macrophages cultured in vitro and, if so, whether macrophages can be activated to inhibit the multiplication. a reticulotropic strain of t. cruzi was capable of infecting human monocytes and monocyte-derived macrophages. intracellular multiplication was observed in both cell types when they were examined microscopically. an increase in the number of trypanosomes occurred in the super ... | 1977 | 321338 |
| establishment and characterization of a cell line (btc-32) from the triatomine bug, triatoma infestans (klug) (hemiptera: reduviidae). | the establishment of a cell line from embryonic tissues of the triatomine bug, triatoma infestans is described. the cell line consists of three cell types which are described, and has a population doubling time of 48 hours during the logarithmic phase of growth. a proportion of the cells remain in the medium as floaters. of the chromosome preparations that could be counted, the majority contained the diploid (2n = 22) number. the cell line has undergone 129 subcultures and has been maintained fo ... | 1977 | 322623 |
| influence of the stage of infection of trypanosoma cruzi in guinea-pigs on infectivity to rhodnius prolixus. | | 1977 | 322624 |
| lipopeptidophosphoglycan from trypanosoma cruzi. amide and ester-linked fatty acids. | lipopeptidophosphoglycan, extracted from whole cells of epimastigote forms of trypanosoma cruzi, has now been shown to contain 12.6% of fatty acids in addition to the previously identified content of neutral sugars (60%), glucosamine (0.8%), peptide (9.5%) and acid-hydrolyzable phosphate (2%). the main fatty acids are palmitic (6.9%) and lignoceric (4.6%) acids. stearic (0.55%), oleic (0.15%) and myristic (0.18%) acids were also found. one third of the fatty acids are bound in the lipopeptidopho ... | 1977 | 323009 |
| [agglutination reaction of t. cruzi, t. cruzi like strains, t. rangeli and t. conorhini with soja hispida lectin and aaptos papillata protectin (author's transl)]. | protectin from the sponge aaptos papillata (keller) was used in the characterization of five strains of t. cruzi (venezuela, guatemala, y. brasilien, peru, wien) and six t. cruzi like strains (triatoma, maryland, itmap 943, fh4, fh5, ln). based upon their membrane receptors, these t. cruzi and t. cruzi like isolates could be differentiated from rangeli (venezuela strain) and t. conorhini (hawai strain) by agglutination reaction to the proctectin. furthermore, after pronase treatment t.rangeli co ... | 1977 | 324052 |
| attempts to define a medium for the in vitro cultivation of trypanosoma cruzi. | | 1977 | 324409 |
| 5-fluorocytosine, a valuable fungicidal agent in specific culture media. | | 1977 | 324410 |
| heterogeneity of the kinetoplast dna molecules of trypanosoma cruzi. | kinetoplast dna (kdna) of the culture form of trypanosoma cruzi is cleaved by restriction endonucleases (hpaii, hindii, ecori, and haeiii). the analysis of the cleavage patterns proves that the minicircles (free circulargenome units) are heterogeneous in base sequences. the same results are obtained with the complex kdna network which is composed of the association of minicircles and linear molecules. kinetic studies of the renaturation of kdna previously cleaved by hpaii into fragments of the g ... | 1977 | 324518 |
| trypanosoma cruzi: ultrastructural and metabolic alterations of epimastigotes by beta-lapachone. | | 1977 | 324785 |
| complement receptors and cell associated complement components. | membrane receptors for activated complement components are widely distributed amongst tissue cells of most mammalian species. common amongst these are receptors for c3b which mediate many of the biological functions of c3. in addition, the genetic control of certain complement components is linked to the genes which code for the major histocompatibility complex. many of these components are also present on cell surfaces. this suggests that the function of the complement system and the major hist ... | 1977 | 324894 |
| intraspecific variation in trypanosoma cruzi: effect of temperature on the intracellular differentiation in tissue culture. | inhibition of t. cruzi amastigote-trypomastigote differentiation in tissue culture at 37 c is a strain-dependent event. when eight t. cruzi strains were submitted to two environmental temperatures (33 and 37 c), the following patterns of differentiation were obtained: in three strains, transformation was inhibited at 37 c but readily occurred at 33 c; in three other strains differentiation took place at both temperatures; finally, in the two remaining strains, a partial inhibition was detected a ... | 1977 | 325187 |