| exploring redundancy in the yeast genome: an improved strategy for use of the cre-loxp system. | gene families having more than three members are a common phenomenon in the saccharomyces cerevisiae genome. as yeast research enters the post-genome era, the development of existing deletion strategies is crucial for tackling this apparent redundancy, hence a method for performing rapid multiple gene disruptions in this organism has been developed. we constructed three replacement cassettes in which different selectable markers were placed between two loxp loci. multiple deletions (of members o ... | 2000 | 10903444 |
| biodiversity of killer activity in yeasts isolated from the brazilian rain forest. | the occurrence of killer activity against a panel composed of 22 industrially and (or) medically important yeasts was investigated in 438 yeast and yeast-like cultures belonging to 96 species, isolated from different environments of the brazilian rain forest. altogether, 26% of ascomycetes, 56% of basidiomycetes, and 42% of yeast-like cultures exhibited killer activity against at least one of the panel yeasts. more than 15 species never reported before as toxin producers were found, with pseudoz ... | 2000 | 10932353 |
| competition between n-alkane-assimilating yeasts and bacteria during colonization of sandy soil microcosms. | an n-alkane-assimilating strain of candida tropicalis was selected in sandy soil inoculated with microorganisms from contaminated sites. competition experiments with n-alkane utilizers from different strain collections confirmed that yeasts overgrow bacteria in sandy soil. acidification of the soil is one of the colonization factors useful for the yeasts. it can be counteracted by addition of bentonite, a clay mineral with high ion exchange capacity, but not, however, by kaolin. strains of diffe ... | 2000 | 10952016 |
| ray38p, a homolog of a purine motif triple-helical dna-binding protein, stm1p, is a ribosome-associated protein and dissociated from ribosomes prior to the induction of cycloheximide resistance in candida maltosa. | cycloheximide (cyh) resistance in candida maltosa is dependent on the induction of a ribosomal protein, q-type l41, the 56th residue of which is glutamine, not proline as in ordinary p-type l41. we found that a 38-kda protein in a wild-type c. maltosa ribosomal fraction became undetectable upon cyh treatment but detectable again with the establishment of cyh resistance by the induction of q-type l41. we cloned a gene coding for this protein and named it ray38 (ribosome-associated protein of yeas ... | 2001 | 11374890 |
| microbiology and physiology of cachaça (aguardente) fermentations. | cachaça (aguardente) is a rum-style spirit made from sugar cane juice by artisanal methods in brazil. a study was made of the production, biochemistry and microbiology of the process in fifteen distilleries in sul de minas. identification of 443 yeasts showed saccharomyces cerevisiae to be the predominant yeast but rhodotorula glutinis and candida maltosa were predominant in three cases. bacterial infection is a potential problem, particularly in older wooden vats, when the ratio of yeasts:bacte ... | 2001 | 11392489 |
| molecular characterization of the hansenula polymorpha fld1 gene encoding formaldehyde dehydrogenase. | glutathione-dependent formaldehyde dehydrogenase (fld) is a key enzyme required forthe catabolism of methanol as a carbon source and certain primary amines, such as methylamine as nitrogen sources in methylotrophic yeasts. here we describe the molecular characterization of the fld1 gene from the yeast hansenula polymorpha. unlike the recently described pichia pastoris homologue, the h. polymorpha gene does not contain an intron. the predicted fld1 product (fld1p) is a protein of 380 amino acids ... | 2002 | 11754481 |
| significance of physical attachment of fungi for bio-treatment of water. | the inhibitory effect of xenobiotics known to damage cell surface structures was studied. the sensitivity of suspended cells of the two fungi candida maltosa and fusarium proliferatum was compared with that of artificial or natural biofilms of these fungi. the results obtained indicate that the resistance of attached cell populations to model xenobiotics is increased compared with suspended cells. only the attached fungal cells had the capacity to degrade acetone and phenol and to adapt to incre ... | 2001 | 11770857 |
| large-scale screening of selected candida maltosa, debaryomyces hansenii and pichia anomala killer toxin activity against pathogenic yeasts. | the killer activity of selected crude toxins produced by nine candida maltosa, debaryomyces hansenii and pichia anomala strains was tested at 37 degrees c against 383 strains belonging to 19 pathogenic species of 10 genera (candida, clavispora, cryptococcus, filobasidiella, issatchenkia, kluyveromyces, pichia, saccharomyces, stephanoascus and trichosporon). the broad killer spectra exhibited by all crude toxins may lead to the development of new antimycotic agents against medically important yea ... | 2001 | 11798052 |
| conservation of a portion of the s. cerevisiae ure2p prion domain that interacts with the full-length protein. | the [ure3] prion of saccharomyces cerevisiae is a self-propagating inactive amyloid form of the ure2 protein. ure2p residues 1-65 constitute the prion domain, and the remaining c-terminal portion regulates nitrogen catabolism. we have examined the ure2 genes of wild-type isolates of s. cerevisiae and those of several pathogenic yeasts and a filamentous fungus. we find that the normal function of the s. cerevisiae ure2p in nitrogen regulation is fully complemented by the ure2p of candida albicans ... | 2002 | 12177423 |
| [selenium tolerance of yeasts]. | selenium tolerance of yeasts widely varies: the growth of some yeasts can be inhibited by a selenium concentration as low as 10(-4) m, whereas others can grow in the presence of 10(-1) m selenium. homogeneous yeast taxa are characterized by a certain level of selenium tolerance, and heterogeneous taxa show a variable level of tolerance to selenium. in general, ascomycetous yeasts are more tolerant to selenium than basidiomycetous yeasts. among the ascomycetous yeasts, the genera dekkera and schi ... | 2002 | 12244713 |
| pseudohyphal growth in a dimorphic yeast, candida maltosa, after disruption of the c-gcn4 gene, a homolog of saccharomyces cerevisiae gcn4. | the transcriptional activator protein gcn4p increases the transcription of many genes that code for amino acid synthesis genes during amino acid starvation in saccharomyces cerevisiae. here we showed that after the disruption of c-gcn4, a homolog in candida maltosa of gcn4 in s. cerevisiae, formed pseudohyphae in minimal medium. this is the first report that a gcn4 homolog is involved in the control of morphological transition. | 2002 | 12400694 |
| inorganic tin and organotin interactions with candida maltosa. | as a consequence of the widespread industrial and agricultural applications of organotins, contamination of various ecosystems has occurred in recent decades. understanding how these compounds interact with microorganisms is important in assessing the risks of organotin pollution. the organotins, tributyltin (tbt), trimethyltin (tmt) and inorganic tin, sn(iv), were investigated for their physical interactions with non-metabolising cells and protoplasts of the yeast candida maltosa, an organism t ... | 2004 | 12879303 |
| the diversity of yeasts in the agricultural soil. | one hundred and eleven yeast strains were isolated from 60 agricultural soil samples. the samples were taken from four various fields located in the southwest of slovakia. cryptococcus laurentii, candida maltosa, metschnikowia pulcherrima, and sporobolomyces salmonicolor were the predominant species in the samples collected from all four types of fields. these species represented 78.4-86.6% of the total yeast counts.the results obtained enabled comparisons to be made between forest and agricultu ... | 2003 | 12964187 |
| cr(vi) reduction in a chromate-resistant strain of candida maltosa isolated from the leather industry. | a cr(vi)-resistant yeast was isolated from tanning liquors from a leather factory in leon, guanajuato, mexico. based on morphological and physiological analyses and the d1/d2 domain sequence of the 26s rdna, the yeast was identified as candida maltosa. resistance of the strain to high cr(vi) concentrations and its ability to chemically reduce chromium was studied. when compared to the three laboratory yeasts candida albicans, saccharomyces cerevisiae and yarrowia lipolytica, the c. maltosa strai ... | 2004 | 15028877 |
| a gcn4p homolog is essential for the induction of a ribosomal protein l41 variant responsible for cycloheximide resistance in the yeast candida maltosa. | cycloheximide (cyh) resistance in the yeast candida maltosa is based on the inducible expression of genes encoding a variant of ribosomal protein l41-q, with glutamine at position 56 instead of the proline found in normal l41. the promoter of l41-q2a, one of the l41-q gene alleles encoding l41-q, has an element similar to the gcn4p-responsive element of saccharomyces cerevisiae. in a previous study, this element was shown to be essential for the induction of l41-q by cyh. in the present study, a ... | 2004 | 15039451 |
| biodegradation of nonionic surfactants. i. biotransformation of 4-(1-nonyl)phenol by a candida maltosa isolate. | results are reported concerning biodegradation of 4-(1-nonyl)phenol by cultures of a candida maltosa strain isolated from aerobic sludge samples collected at a depuration plant treating wastewaters from a textile industry. the yeast was able to utilize 4-(1-nonyl)phenol as a sole carbon and energy source. preliminary attempts to draw the actual metabolic pathway evidenced microbial attack on the alkyl chain with the production of 4-acetylphenol. to the best of our knowledge this is the first rep ... | 1995 | 15091504 |
| [zeta-potential of n-alkane emulsion droplets and its role in substrate transport into yeast cells]. | zeta-potential of n-alkane droplets, formed by fatty acids, were studied in model systems of culture liquid of yeasts (candida maltosa) capable of utilizing n-alkanes. the value of zeta-potential was found to depend on the droplet size. the negative zeta-potential of submicron droplets was so high that it prevented the droplets from being coagulated with cells possessing a high negative zeta-potential. the dominant role of submicron n-alkane droplets in the kinetics of yeast growth could be acco ... | 2004 | 15283336 |
| comparison of yeast (candida maltosa) and bacterial (rhodococcus erythropolis) phenol hydroxylase activity and its properties in the phenolic compounds biodegradation. | aromatic contaminants of the environment, to which belongs phenol and its derivatives, are toxic and in most of the cases hard to degrade. removal of these pollutants by biological, gentle and effective way, depends on specific environmental conditions in the locality and on the biodegradation potential of the used microbial population. closer characterization of the biodegradation and enzyme mechanisms is therefore an essential assumption of the successful implementation of microbes. this paper ... | 2003 | 15296151 |
| the enhancement of reproduction and biodegradation activity of eukaryiotic cells by humic acids. | fourteen samples of humic acids (ha) were screened for ability to influence reproduction and biodegradation activity of eukaryotic cells in the presence of chosen toxic pollutants. microorganisms candida maltosa and rhodotorula mucilaginosa (soil isolates) were used for all tests. it was observed during our experiments that some samples of humic acids served as a protection against the high concentration of toxic pollutants (phenol, naphtalene etc). this effect can be widely used in many bioreme ... | 2003 | 15296162 |
| role of speciation in organotin toxicity to the yeast candida maltosa. | assessment of organometal pollution requires an understanding of the various processes that influence the bioavailability and toxicity of the contaminant. organotins may exist as both cationic species and neutral hydroxides in aqueous solution, with the formation of chloride species in the presence of cl-. although these species have different chemical properties, there is very little information on the influence of speciation on organotin and microbial cell interactions. tributyltin (tbt) and t ... | 2004 | 15298196 |
| colonization of surfaces by phenolic compounds utilizing microorganisms. | the aim of the present study is to determine optimal adhesive interaction of phenolic compounds utilizing candida maltosa and rhodococcus erythropolis when adhering to kaolin, silicone, synthetic foil (steriking r40) and fluorinated silicones, comparing cell and support surface hydrophobicity. in parallel, the interfering effect of detergents was investigated. data obtained show that the less hydrophobic supports display high initial cell adhesion when contacted with the cell type with a lower s ... | 2005 | 15661283 |
| cloning and heterologous expression of the nadph cytochrome p450 oxidoreductase genes from an industrial dicarboxylic acid-producing candida tropicalis. | nadph cytochrome p450 oxidoreductase (cpr) catalyses the transfer of electrons during p450-mediated oxidation, which plays an important role in the omega-oxidation pathway of candida tropicalis. two putative allelic genes, cpr-a and cpr-b, were cloned from the long chain dicarboxylic acid-producing candida tropicalis 1230, using cassette pcr methods. both the identified open reading frames predict the gene products of 679 amino acid residues. the deduced amino acid sequences of cpr-a and cpr-b a ... | 2005 | 15849785 |
| candida yeast long chain fatty alcohol oxidase is a c-type haemoprotein and plays an important role in long chain fatty acid metabolism. | the industrial yeasts candida tropicalis or candida cloacae are able to grow on a variety of long chain alkanes and fatty acids as the sole carbon source. the complete oxidation of these substrates involves two sequential oxidative pathways: omega-oxidation, comprising the p450 alkane oxidase, a flavin-dependent membrane-bound long chain fatty alcohol oxidase [fao] and a possible separate aldehyde oxidase [f.m. dickinson, c. wadforth, purification and some properties of alcohol oxidase from alka ... | 2005 | 16046182 |
| antimicrobial, cytotoxic and antioxidant activity of selected basidiomycetes from yemen. | dichloromethane, methanol and aqueous extracts of 23 selected basidiomycetes species fruiting bodies collected in yemen were screened in vitro for their antibacterial activities against three gram-positive bacteria (staphyloccocus aureus, bacillus subtilis, micrococcus flavus), two gram-negative bacteria (escherichia coli, pseudomonas aeruginosa) and against one yeast fungus (candida maltosa), as well as for their cytotoxic and antioxidant activity. the highest antibacterial activity was shown b ... | 2005 | 16259127 |
| metal tolerance of yeasts isolated from water, soil and plant environments. | the tolerance of seventy yeast strains belonging to 15 species, isolated from water and soil environments as well as from tree leaves, to four heavy metals--copper, zinc, nickel and cadmium were studied. we have found that the interspecific and intraspecific variations in metal tolerance among studied strains were considerable. the highest interspecific variations were observed toward copper and cadmium. the strains of the species sporobolomyces salmonicolor, cryptococcus albidus, cystofilobasid ... | 2006 | 16598828 |
| cloning and molecular characterization of a gene coding d-xylulokinase (cmxyl3) from candida maltosa. | to clone and identify a gene (cmxyl3) coding d-xylulokinase from candida maltosa xu316 and understand its physiological function. | 2006 | 16834601 |
| application of pcr-dgge to analyse the yeast population dynamics in slurry reactors during degradation of polycyclic aromatic hydrocarbons in weathered oil. | slurry-phase reactors have been used to investigate the biodegradation feasibility of polycyclic aromatic hydrocarbons (pahs) in weathered crude oil, by mixed culture containing five pahs-degrading yeast strains. yeasts were isolated from the oily soil by enrichment culture, using phenanthrene as a sole carbon source, and identified based on the 26s ribosomal dna (rdna) sequence. yeast strains belonged to the genera candida, pichia, rhodotorula and sporidiobolus. the experiment was carried out f ... | 2006 | 17001616 |
| screening and characterization of yeasts for xylitol production. | to discover novel naturally occurring xylitol producing yeast species with potential for industrial applications. | 2006 | 17040233 |
| biological control of peach fungal pathogens by commercial products and indigenous yeasts. | the potential use of the commercial biocontrol products serenade (bacillus subtilis qst-713) and trichodex (trichoderma harzianum rifai strain t39) to inhibit the postharvest pathogenic molds penicillium crustosum and mucor circinelloides was investigated. both products exhibited antagonistic activity in vitro against the pathogens, reducing their growth at different levels. in addition, epiphytic yeasts isolated from peaches were identified as candida maltosa, pichia fermentans, and pichia kluy ... | 2006 | 17066929 |
| antioxidant, antimicrobial and cytotoxic activities of selected medicinal plants from yemen. | ninety crude extracts, including dichloromethane, methanol and aqueous extracts from 30 medicinal plants used in the yemeni ethnomedicine to treat common infections, were screened in vitro for antimicrobial activities against three gram-positive bacteria and two gram-negative bacteria, candida maltosa and five opportunistic human fungal pathogens (two yeasts, three hyphomycetes). most of the plants showed antibacterial activities. extracts from tamarindus indica flowers and ficus vasta fruits ha ... | 2007 | 17306942 |
| yeast and bacteria cell hydrophobicity and hydrocarbon biodegradation in the presence of natural surfactants: rhamnolipides and saponins. | this study concerns the relation between hydrocarbon biodegradation in the presence of natural surfactants and cell hydrophobicity resulting from the use of these surfactants. the relative capabilities of two bacterial strains (pseudomonas aeruginosa and bacillus subtilis) and two yeast strains (candida maltosa, yarrowia lipolytica) were investigated. the selected microorganisms were tested separately and in combination in order to achieve the optimal degrading performance. the surface cell hydr ... | 2008 | 17959375 |
| yeasts isolated from plant-associated beetles and other insects: seven novel candida species near candida albicans. | yeasts related to candida albicans were isolated from the digestive tracts of beetles in eight families and various orders of insects such as earwigs, crickets, and roaches, most of which were caught at light traps or in a few cases directly from plant materials. based on comparisons of dna sequences and other taxonomic characteristics, a total of 41 isolates were identified as candida orthopsilosis, candida pseudorhagii, candida maltosa, candida parapsilosis, candida tropicalis, candida neerlan ... | 2008 | 17986254 |
| biosorption of nickel by yeasts in an osmotically unsuitable environment. | the tolerance, sorption of nickel(ii) ions, and changes in the production and composition of exopolymers of eight yeast strains grown under nickel presence with/without nacl were studied. strains of pichia anomala and candida maltosa known as the most resistant yeasts against nickel tolerated up to 3 mm ni2+. nacl addition decreased both the resistance of the yeast strains toward nickel ions and the sorption of metal ions into cells. all yeasts absorbed nickel predominantly into exopolymers (gly ... | 2008 | 19227837 |
| regulation of acanthamoeba castellanii alternative oxidase activity by mutual exclusion of purine nucleotides; atp's inhibitory effect. | the effects of different adenine and guanine nucleotides on the cyanide-resistant respiration (i.e. alternative oxidase (acaox) activity) of mitochondria from the amoeba a. castellanii mitochondria were studied. we found that guanine nucleotides activate acaox to a greater degree than adenine nucleotides, and that nucleoside monophosphates were more efficient activators than nucleoside di- or triphosphates. the extent of the nucleotides' influence on acaox was dependent on the medium's ph and wa ... | 2009 | 19366609 |
| synthesis of model morpholine derivatives with biological activities by laccase-catalysed reactions. | the efficient enzyme-catalysed reaction of morpholines as model structures for bioactive compounds with para-dihydroxylated aromatic systems was carried out using the oxidoreductase laccase and atmospheric oxygen to produce eight novel morpholine-substituted aromatics. the laccase of myceliophthora thermophila was used for cross-linking morpholines containing primary or secondary amino groups with para-dihydroxylated laccase substrates. we demonstrate that not only primary amino groups, but also ... | 2009 | 19788410 |
| the alcohol dehydrogenase system in the xylose-fermenting yeast candida maltosa. | the alcohol dehydrogenase (adh) system plays a critical role in sugar metabolism involving in not only ethanol formation and consumption but also the general "cofactor balance" mechanism. candida maltosa is able to ferment glucose as well as xylose to produce a significant amount of ethanol. here we report the adh system in c. maltosa composed of three microbial group i adh genes (cmadh1, cmadh2a and cmadh2b), mainly focusing on its metabolic regulation and physiological function. | 2010 | 20668703 |
| microorganisms form exocellular structures, trophosomes, to facilitate biodegradation of oil in aqueous media. | cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. among the microorganisms studied here, the yeasts (schwanniomyces occidentalis, torulopsis candida, candida tropicalis, candida lipolytica, candida maltosa, candida paralipolytica) and two representative bacteria (rhodococcus sp. and pseudomonas putida) produced exocellular structures compos ... | 2010 | 21182540 |
| xylitol production from xylose mother liquor: a novel strategy that combines the use of recombinant bacillus subtilis and candida maltosa. | xylose mother liquor has high concentrations of xylose (35%-40%) as well as other sugars such as l-arabinose (10%-15%), galactose (8%-10%), glucose (8%-10%), and other minor sugars. due to the complexity of this mother liquor, further isolation of xylose by simple method is not possible. in china, more than 50,000 metric tons of xylose mother liquor was produced in 2009, and the management of sugars like xylose that present in the low-cost liquor is a problem. | 2011 | 21299871 |
| oxygen limitation induces indirectly the synthesis of cytochrome p-450 mrna in alkane-growing candida maltosa. | candida maltosa cells grown on hexadecane under oxygen limitation have an up to 6-fold higher cytochrome p-450 content in comparison to cells cultivated at oxygen saturation. we show by mrna quantification using an in vitro translation system and subsequent specific immunoprecipitation that the cytochrome p-450 induction occurs mainly on the transcriptional level. the signal for induction may be the enhanced intracellular hexadecane concentration owing to a reduced hydroxylation capacity of the ... | 2011 | 3342052 |
| metabolism of alkenes and ketones by candida maltosa and related yeasts. | knowledge is scarce about the degradation of ketones in yeasts. for bacteria a subterminal degradation of alkanes to ketones and their further metabolization has been described which always involved baeyer-villiger monooxygenases (bvmos). in addition, the question has to be clarified whether alkenes are converted to ketones, in particular for the oil degrading yeast candida maltosa little is known. in this study we show the degradation of the aliphatic ketone dodecane-2-one by candida maltosa an ... | 2014 | 25309846 |
| the influence of rhamnolipids on aliphatic fractions of diesel oil biodegradation by microorganism combinations. | twelve different bacteria-yeast combinations were tested for determination of their ability to biodegrade diesel oil. the cell surface properties of the bacterial and yeast strains were correlated with the type of carbon source used in the experiments. the highest biodegradation of diesel oil after 7 days was obtained for the following combinations: aeromonas hydrophila mr4-yarrowia lipolytica eh 56 (87 %) and xantomonas maltophila mrp7-candida maltosa eh15 (90 %). degradation performances of 10 ... | 2012 | 24426083 |
| sporadic distribution of prion-forming ability of sup35p from yeasts and fungi. | sup35p of saccharomyces cerevisiae can form the [psi+] prion, an infectious amyloid in which the protein is largely inactive. the part of sup35p that forms the amyloid is the region normally involved in control of mrna turnover. the formation of [psi+] by sup35p's from other yeasts has been interpreted to imply that the prion-forming ability of sup35p is conserved in evolution, and thus of survival/fitness/evolutionary value to these organisms. we surveyed a larger number of yeast and fungal spe ... | 2014 | 25081567 |
| balticidins a-d, antifungal hassallidin-like lipopeptides from the baltic sea cyanobacterium anabaena cylindrica bio33. | balticidins a-d (1-4), four new antifungal lipopeptides, were isolated from the laboratory-cultivated cyanobacterium anabaena cylindrica strain bio33 isolated from a water sample collected from the baltic sea, rügen island, germany. fractionation of the 50% aqueous meoh extract was performed by bioassay-guided silica gel column chromatography followed by spe and repeated reversed-phase hplc. the main fraction containing the compounds exhibited a strong and specific antifungal activity with inhib ... | 2014 | 24937366 |
| biological activities of selected basidiomycetes from yemen. | in a previous paper we demonstrated the results of biological screening of yemeni basidiomycetes. the present study was aimed to investigate the antimicrobial and the antioxidant activity of further basidiomycetes collected in yemen. dichloromethane, methanol and aqueous extracts of the fruiting bodies of 25 species were screened in vitro for their antibacterial activities against three gram-positive bacteria (staphyloccocus aureus, bacillus subtilis, micrococcus flavus) and two gram-negative ba ... | 2013 | 23556343 |
| yeasts from phylloplane and their capability to produce indole-3-acetic acid. | yeasts were isolated from the phylloplane of various plant species collected from seven provinces in thailand. a total of 114 yeast strains and 10 strains of a yeast-like fungus were obtained by enrichment isolation from 91 out of 97 leaf samples (93.8 %). on the basis of the d1/d2 domain of the large subunit rrna gene sequence similarity, 98 strains were identified to be of 36 yeast species in 18 genera belonging to ascomycota viz. candida, clavispora, cyberlindnera, debaryomyces, hanseniaspora ... | 2012 | 22886557 |
| modifications of the cell wall of yeasts grown on hexadecane and under starvation conditions. | electron-microscopic examinations have demonstrated local modifications in the cell wall of the yeast candida maltosa grown on hexadecane. in our earlier studies, these modified sites, observed in other yeasts grown on oil hydrocarbons, were conventionally called 'canals'. the biochemical and cytochemical studies of c. maltosa have revealed a correlation between the formation of 'canals' and decrease in the amount of cell wall polysaccharides, glucan and mannan. the ultrathin sections and surfac ... | 2016 | 26833628 |
| manganese tolerance in yeasts involves polyphosphate, magnesium, and vacuolar alterations. | basidiomycetous and ascomycetous yeast species were tested for manganese tolerance. basidiomycetous cryptococcus humicola, cryptococcus terricola, cryptococcus curvatus and ascomycetous candida maltosa, kluyveromyces marxianus, kuraishia capsulata, lindnera fabianii and sacharomyces cerevisiae were able to grow at manganese excess (2.5 mmol/l), while the growth of basidiomycetous rhodotorula bogoriensis was completely suppressed. the lag phase duration increased and the exponential growth rate d ... | 2016 | 26646947 |
| candida baotianmanensis sp. nov. and candida pseudoviswanathii sp. nov., two ascosporic yeast species isolated from the gut of beetles. | four yeast strains were isolated from the gut of beetles collected on baotianman mountain and people's park of nanyang in henan province, china. these strains produced unconjugated asci with one or two ellipsoidal to elongate ascospores in a persistent ascus. phylogenetic analysis of the d1/d2 domains of the lsu rrna gene sequences indicated that the isolates represent two novel sexual species in the candida/lodderomyces clade. candida baotianmanensis sp. nov. was located in a statistically well ... | 2015 | 26297152 |
| isolation and characterization of l-valine-degrading candida maltosa dlpu-zpb for d-valine preparation from dl-valine. | to develop a practical process for d-valine preparation from dl-valine, l-valine was used as a sole source of carbon and nitrogen in basal minimal medium to isolate l-valine-degrading micro-organisms. a yeast strain dlpu-zpb was obtained, which showed asymmetric degrading activity against dl-valine. based on the morphology, physiological and biochemical characteristics, and 26s rdna d1/d2 domain sequence, strain dlpu-zpb was identified as candida maltosa. the cells of this strain were used as a ... | 2015 | 26250528 |
| microbial production of 1α-hydroxyvitamin d3 from vitamin d3. | the microbial transformation of vitamin d3 (1) by the fungi candida maltosa r42 and botrytis allii nrrl 2502 was investigated. incubation of compound 1 with c. maltosa r42 and b. allii nrrl 2502 produced the same three more polar metabolites in small yields. the main metabolite was identified as 1α-hydroxyvitamin d3 (2). this biotransformation has utility as a possible tool for the production of 1α-hydroxyvitamin d3 from the readily available vitamin d3 for patients with compromised kidney funct ... | 2014 | 24456427 |
| identification and characterization of a gene encoding an abc transporter expressed in the dicarboxylic acid-producing yeast candida maltosa. | a gene, cmcdr1, encoding an abc transporter of the dicarboxylic acid (dca)-producing yeast candida maltosa was cloned. transcription of cmcdr1 was upregulated in a dca-hyper-producing mutant of c. maltosa in a later phase of culture on n-dodecane, but not in its parental strain. cmcdr1 expression was significantly induced by the longer-chain dca in this mutant. | 2013 | 24317069 |
| biosensor analyzer for bod index express control on the basis of the yeast microorganisms candida maltosa, candida blankii, and debaryomyces hansenii. | the parameters of biosensors based on the yeast strains candida maltosa vkm y-2359, candida blankii vkm y-2675, and debaryomyces hansenii vkm y-2482 for biochemical oxygen demand (bod) detection are compared. the catalytic activity of the strains was analyzed in relation to the growth phase. the possibility of using d. hansenii as a basis for receptor element of a biosensor for bod detection in municipal and biotechnological wastewaters was shown. | 2012 | 22418260 |
| enhanced induction of cytochromes p450alk that oxidize methyl-ends of n-alkanes and fatty acids in the long-chain dicarboxylic acid-hyperproducing mutant of candida maltosa. | in the long-chain dicarboxylic acids (dca)-hyperproducing mutant candida maltosa strains, methyl-ends of n-alkanes and fatty acids are hydroxylated by n-alkane inducible cytochromes p450 (p450alk), presumably as an essential step in dca production. a significantly higher production of p450alks was observed in response to n-alkane in the dca-hyperproducing mutant strain m2030 than in the wild-type strain 1098. northern analysis demonstrated that n-tetradecane induction levels of mrnas of all four ... | 2007 | 17403051 |
| n-alkane and clofibrate, a peroxisome proliferator, activate transcription of alk2 gene encoding cytochrome p450alk2 through distinct cis-acting promoter elements in candida maltosa. | the alk2 gene, encoding one of the n-alkane-hydroxylating cytochromes p450 in candida maltosa, is induced by n-alkanes and a peroxisome proliferator, clofibrate. deletion analysis of this gene's promoter revealed two cis-acting elements-an n-alkane-responsive element (are2) and a clofibrate-responsive element (cre2)-that partly overlap in sequence but have distinct functions. are2-mediated activation responded to n-alkanes but not to clofibrate and was repressed by glucose. cre2-mediated activat ... | 2005 | 15721276 |
| factors influencing biofilm formation of phenol degrader candida maltosa. | | 2003 | 15296168 |
| evaluation of spray cycle bioreactor using l-alanine degradation by candida maltosa. | the oxygen-supply capability of a spray cycle reactor was evaluated by using it for oxidative degradation of l-alanine. the volumetric oxygen transfer coefficient, kla, was evaluated as a parameter for the oxygen supply. the liquid circulation rate in the spray cycle reactor was represented in terms of the number of circulations. the kla increased with the number of circulations, especially by stirring in the reservoir vessel, reaching 272/h at 4.4/min of circulation numbers. this value was 1.4 ... | 1998 | 9805365 |
| transformation of candida maltosa by electroporation. | | 1995 | 7550747 |
| molecular analysis of a leu2-mutant of candida maltosa demonstrates the presence of multiple alleles. | three different alleles of the beta-isopropylmalate dehydrogenase gene were cloned and sequenced from a leucine auxotrophic mutant, g587, of candida maltosa. the cloning of functionally-intact wild-type genes from this mutant strain suggests the presence of silent gene copies. an interallelic-divergence comparison has provided evidence for new regulatory mechanisms. sequence data and karyotype analysis argue for a highly-aneuploid genome of c. maltosa. an interpretation for the spontaneous auxot ... | 1994 | 7859302 |
| purification and some properties of alanine aminotransferase from candida maltosa. | alanine aminotransferase (alaat; ec 2.6.1.2) was purified to homogeneity from candida maltosa that was grown on l-alanine as the sole source of carbon and nitrogen. the enzyme has a molecular mass of 99kda and consists of two subunits of equal molecular mass (52 kda). each subunit binds one mole of plp. the enzyme has an isoelectric point of 5.3 and an optimum ph of 6.0-7.5. the spectroscopic profile and an inhibition experiment showed that both plp and free-sh groups are directly involved in th ... | 1994 | 7764540 |
| steroid 11ß-hydroxylation by a fungal microsomal cytochrome p450. | the steroid 11ß-hydroxylase activity of the fungus cochliobolus lunatus was increased about 100-fold by cultivation of mycelia for 4-5 h with 20-hydroxymethyl-1,4-pregnadien-3-one. cell-free extracts revealed a maximum activity of 45 nmol 11ß-hydroxyprogesterone/h·mg protein in the 100,000 g pellet fraction. the 11ß-hydroxylation was dependent on nadph. the formation of 11ß-hydroxyprogesterone correlated linearly with the cytochrome p450 concentration. the fungal 11ß-hydroxylase transformed both ... | 1992 | 22217857 |
| efficient electropulse transformation of intact candida maltosa cells by different homologous vector plasmids. | conditions for efficient and quick transformation by electroporation were developed in candida maltosa. to investigate the efficiency of transformation with integrative as well as with autonomously replicating plasmids, a series of vectors was constructed for homologous transformation of this species. transformants were obtained with different plasmids as covalently closed circular molecules and as linearized dna. the influence of recipient strain and plasmid type as well as of cell number and p ... | 1992 | 1332307 |
| valine inhibition of beta-isopropylmalate dehydrogenase takes part in the regulation of leucine biosynthesis in candida maltosa. | the beta-isopropylmalate (ipm) dehydrogenase (ec 1.1.1.85) of candida maltosa, the third pathway-specific enzyme of leucine biosynthesis, was purified, some properties of the enzyme were studied and a novel regulatory pattern was found. the km values of the enzyme were estimated to be 0.42 mm for beta-ipm and 0.34 mm for nad+. it is demonstrated that the enzyme can be regulated by l-valine. the inhibition was competitive with respect to beta-ipm (ki = 1.84 mm) and non-competitive with respect to ... | 1991 | 1804028 |
| cyp52 (cytochrome p450alk) multigene family in candida maltosa: molecular cloning and nucleotide sequence of the two tandemly arranged genes. | southern blot analysis under low-stringency conditions using a previously isolated n-alkane-inducible cytochrome p450 (p450alk) gene as a probe revealed the presence of multiple p450alk-related genes in the genome of candida maltosa. nine p450alk-related genes (one reported previously and eight in the present report) were isolated from a genomic library constructed from this strain, and these were classified on the basis of sequence similarities into three pairs of putative allelic genes and thr ... | 1991 | 2039569 |
| [the use of various mutagens for induction of nystatin-resistant mutants in candida maltosa]. | the mutagenic activity of nitrous acid, 6-n-hydroxylaminopurine, n-methyl-n'-nitro-n-nitrosoguanidine and 4-n-nitroquinoline oxide was studied for candida maltosa. the efficacy of their action on c. maltosa cells was determinded in order to obtain nystatin-resistant strains. | 2006 | 2635266 |
| regulatory properties of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase isozymes from candida maltosa. | | 1985 | 2860237 |
| absolute dependence of phenylalanine and tyrosine biosynthetic enzyme on tryptophan in candida maltosa. | candida maltosa synthesizes phenylalanine and tyrosine only via phenylpyruvate and p-hydroxyphenylpyruvate. tryptophan is absolutely necessary for the enzymatic reaction of chorismate mutase and prephenate dehydrogenase; activity of prephenate dehydratase can be increased 2.5-fold in the presence of tryptophan. activation of the chorismate mutase, prephenate dehydratase and prephenate dehydrogenase by tryptophan is competitive with respect to chorismate and prephenate with ka 0.06mm, 0.56mm and ... | 1984 | 6479898 |
| cell wall canals formed upon growth of сandida maltosa in the presence of hexadecane are associated with polyphosphates. | canals are supramolecular complexes observed in the cell wall of candida maltosa grown in the presence of hexadecane as a sole carbon source. such structures were not observed in glucose grown cells. microscopic observations of cells stained with diaminobenzidine revealed the presence of oxidative enzymes in the canals. dapi staining revealed that a substantial part of cellular polyphosphate was present in the cell wall of cells grown on hexadecane in condition of phosphate limitation. the conte ... | 2017 | 28475763 |