| overexpression and purification of aspergillus aculeatus beta-mannosidase and analysis of the integrated gene in aspergillus oryzae. | an expression plasmid for the manb gene encoding aspergillus aculeatus beta-d-mannosidase (manb) was constructed by using an expression vector carrying an improved promoter. after transformation of a. oryzae by the plasmid, several transformants formed colonies emitting fluorescence on a plate containing 4-methylumbelliferyl beta-d-mannopyranoside (mu-man) under uv-irradiation. the transformant that displayed the strongest fluorescence, named a. oryzae bmn1, produced about 270 mg manb/l in liqui ... | 2001 | 16233072 |
| cloning and transcription analysis of the aspergillus aculeatus no. f-50 endoglucanase 2 (cmc2) gene. | the cmc2 gene, coding for an endoglucanase 2 (cmc2) of aspergillus aculeatus, was cloned using both genomic and cdna libraries, and sequenced. the gene consists of 1230 bp encoding a protein of 410 amino acid residues with a molecular mass of 43,697 da. the cmc2, composed of an n-terminal catalytic domain belonging to the family 5 of glycosyl hydrolases and a c-terminal cellulose-binding domain (cbd) belonging to the family i of cbds, showed identity with other fungal endoglucanases, particularl ... | 2002 | 16233338 |
| overexpression of aspergillus aculeatus cellobiohydrolase i in aspergillus oryzae. | to express the cbhi gene, encoding aspergillus aculeatus cellobiohydrolase i (cbhi), in aspergillus oryzae, a plasmid was constructed. the strain that displayed the strongest cbhi activity among the transformants produced about 941 mg/l in liquid culture. it was confirmed by a pcr method that the plasmid was integrated at the niad locus. | 2003 | 16233469 |
| beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides. | sugar syrup and molasses from beet processing containing 620 and 570 mg/ml sucrose, respectively, were assayed as low-cost and available substrates for the enzymatic synthesis of fructo-oligosaccharides (foss). a commercial pectinase (pectinex ultra sp-l, from aspergillus aculeatus) characterized by the presence of a transfructosylating activity was used as a biocatalyst. the fos production increased when lowering the initial ph value of syrup (7.5) and molasses (8.9) to 5.5. sugar syrup and mol ... | 2006 | 16608216 |
| gene duplication event in family 12 glycosyl hydrolase from phytophthora spp. | a total of 18 paralogs of xyloglucan-specific endoglucanases (egls) from the glycosyl hydrolase family 12 were identified and characterized in phytophthora sojae and phytophthora ramorum. these genes encode predicted extracellular enzymes, with sizes ranging from 189 to 435 amino acid residues, that would be capable of hydrolyzing the xyloglucan component of the host cell wall. in two cases, four and six functional copies of these genes were found in tight succession within a region of 5 and 18 ... | 2006 | 16784880 |
| an its-rflp method to identify black aspergillus isolates responsible for ota contamination in grapes and wine. | ochratoxigenic mycobiota in grapes from representative wine regions in valencia was identified. black aspergilli were predominant among the different aspergillus spp. isolated. restriction digestion analysis of the its products was tested as a rapid method to identify isolates of black aspergillus species from grapes. restriction endonuclease digestion of the its products using the endonucleases hhai, nlaiii and rsai, distinguished five types of restriction fragment length polymorphism (rflp) co ... | 2007 | 16870292 |
| effect of temperature and high pressure on the activity and mode of action of fungal pectin methyl esterase. | pectin was de-esterified with purified recombinant aspergillus aculeatus pectin methyl esterase (pme) during isothermal-isobaric treatments. by measuring the release of methanol as a function of treatment time, the rate of enzymatic pectin conversion was determined. elevated temperature and pressure were found to stimulate pme activity. the highest rate of pme-catalyzed pectin de-esterification was obtained when combining pressures in the range 200-300 mpa with temperatures in the range 50-55 de ... | 2006 | 17022669 |
| purification and kinetic characterization of a fructosyltransferase from aspergillus aculeatus. | a fructosyltransferase present in pectinex ultra sp-l, a commercial enzyme preparation from aspergillus aculeatus, was purified to 107-fold and further characterised. the enzyme was a dimeric glycoprotein (20% (w/w) carbohydrate content) with a molecular mass of around 135 kda for the dimer. optimal activity/stability was found in the ph range 5.0-7.0 and at 60 degrees c. it was stable or slightly activated (upto 1.4-fold) in the presence of reducing agents, such as dithiothreitol and 2-mercapto ... | 2007 | 17056145 |
| cloning, functional expression and characterization of aspergillus sulphureus beta-mannanase in pichia pastoris. | using rt-pcr and rapid amplication of cdna ends (race) techniques, a 1345bp full-length cdna fragment was obtained from aspergillus sulphureus. the gene, designated mann, codes for a 383-residue with a calculated mass of 41,389da. mann displayed amino acid sequence similarity to the beta-mannanase of aspergillus aculeatus and trichoderma reesei, members of the glycoside hydrolase family 5. the recombinant beta-mannanase gene was successfully expressed in a fully active form in pichia pastoris. s ... | 2007 | 17194495 |
| variability in the structure of rye flour alkali-extractable arabinoxylans. | the variability in rye flour alkali-extractable arabinoxylan (ae-ax) structures was examined by extensive fractionation and enzymic degradation studies. ax were isolated from destarched rye water-unextractables by sequential extraction with saturated barium hydroxide solution, water, 1.0 m sodium hydroxide, and water. the isolated ae-ax contained ca. 51% ax with an arabinose to xylose (a/x) ratio of 0.71. fractionation of the isolated ae-ax by ethanol precipitation yielded a range of ae-ax fract ... | 2007 | 17274627 |
| improving retting of fibre through genetic modification of flax to express pectinases. | flax (linum usitatissimum l.) is a raw material used for important industrial products. linen has very high quality textile properties, such as its strength, water absorption, comfort and feel. however, it occupies less than 1% of the total textile market. the major reason for this is the long and difficult retting process by which linen fibres are obtained. in retting, bast fibre bundles are separated from the core, the epidermis and the cuticle. this is accomplished by the cleavage of pectins ... | 2008 | 17372706 |
| plant cell wall degradation by saprophytic bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization. | plant cell wall degradation is a premier event when bacillus subtilis, a typical saprophytic bacterium, invades plants. here we show the degradation system of rhamnogalacturonan type i (rg-i), a component of pectin from the plant cell wall, in b. subtilis strain 168. strain 168 cells showed a significant growth on plant cell wall polysaccharides such as pectin, polygalacturonan, and rg-i as a carbon source. dna microarray analysis indicated that three gene clusters (yesopqrstuvwxyz, ytepqrst, an ... | 2007 | 17449691 |
| rhamnogalacturonan lyase reveals a unique three-domain modular structure for polysaccharide lyase family 4. | rhamnogalacturonan lyase (rg-lyase) specifically recognizes and cleaves alpha-1,4 glycosidic bonds between l-rhamnose and d-galacturonic acids in the backbone of rhamnogalacturonan-i, a major component of the plant cell wall polysaccharide, pectin. the three-dimensional structure of rg-lyase from aspergillus aculeatus has been determined to 1.5 a resolution representing the first known structure from polysaccharide lyase family 4 and of an enzyme with this catalytic specificity. the 508-amino ac ... | 2004 | 15135077 |
| a comparison of three xylanases on the nutritive value of two wheats for broiler chickens. | three xylanase products, xylanase a derived from thermomyces lanuginosus, xylanase b from humicola insolens and xylanase c from aspergillus aculeatus, were examined for their effects on the nutritive value of wheat. the study investigated the effects of enzyme addition to broiler diets based on a low-metabolisable-energy (me) wheat and a normal-me wheat, with the emphasis on changes in composition of the nsp along the digestive tract in broiler chickens. there were significant (p<0.01) enzyme an ... | 2004 | 15230987 |
| crystallization and preliminary x-ray studies of rhamnogalacturonase a from aspergillus aculeatus. | recombinant rhamnogalacturonase a from aspergillus aculeatus has been crystallized and x-ray diffraction data has been collected. crystals were grown by the hanging-drop vapour-diffusion technique, under the conditions 10% peg 8000, 0.05 m kh(2)po(4) and 0.1 m sodium acetate buffered at ph 3.5. the crystals diffract beyond 2.0 a resolution and belong to one of the orthorhombic space groups i2(1)2(1)2(1) or i222, with the unit-cell parameters a = 62.9, b = 125.4 and c = 137.0 a. there is one mole ... | 1997 | 15299976 |
| the structure of endo-beta-1,4-galactanase from bacillus licheniformis in complex with two oligosaccharide products. | the beta-1,4-galactanase from bacillus licheniformis (blgal) is a plant cell-wall-degrading enzyme involved in the hydrolysis of beta-1,4-galactan in the hairy regions of pectin. the crystal structure of blgal was determined by molecular replacement both alone and in complex with the products galactobiose and galactotriose, catching a first crystallographic glimpse of fragments of beta-1,4-galactan. as expected for an enzyme belonging to gh-53, the blgal structure reveals a (betaalpha)(8)-barrel ... | 2004 | 15312766 |
| development of an efficient production method for beta-mannosidase by the creation of an overexpression system in aspergillus aculeatus. | to develop an overexpression system in aspergillus aculeatus in order to establish an efficient overproduction method of beta-mannosidase (manb). | 2007 | 17651209 |
| purification and characterization of a new endoglucanase from aspergillus aculeatus. | endoglucanase has been isolated from aspergillus aculeatus. the purified enzyme showed a single band and had a molecular weight of 45,000 da as indicated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with a specific activity of 1.4 units/mg. the purified enzyme was identified as endoglucanase, showing a high specific activity toward cm-cellulose and low specific activity toward avicel. the activity of the isolated enzyme was optimum at a ph of 5.0 and temperature of 40 degrees c, ... | 2007 | 17685540 |
| yest: a new rhamnogalacturonan acetyl esterase from bacillus subtilis. | yest, a putative protein from bacillus subtilis atcc 6633 that has been provisionally classified as a rhamnogalacturonan acetyl esterase (rgae) in ce-12 family, was cloned, expressed in escherichiacoli rosetta (de3), and purified. the enzyme is monomeric with a molecular mass of 37 kda and presents thermophilic properties similar to rgae from aspergillus aculeatus, although yest is more alkaliphilic. the study of inhibitors confirmed the importance of the his and the nucleophilic ser for the est ... | 2008 | 17957779 |
| a xyloglucan-specific family 12 glycosyl hydrolase from aspergillus niger: recombinant expression, purification and characterization. | a new gh12 (glycosyl hydrolase 12) family xeg [xyloglucan-specific endo-beta-1,4-glucanase (ec 3.2.1.151)] from aspergillus niger, anxeg12a, was overexpressed, purified and characterized. whereas seven xyloglucanases from gh74 and two xyloglucanases from gh5 have been characterized previously, this is only the third characterized example of a gh12 family xyloglucanase. gh12 enzymes are structurally and mechanistically distinct from gh74 enzymes. although over 100 gh12 sequences are now available ... | 2008 | 18072936 |
| characterization of a new rhamnogalacturonan acetyl esterase from bacillus halodurans c-125 with a new putative carbohydrate binding domain. | bh1115 is a gene from bacillus halodurans strain c-125 that hypothetically encodes a rhamnogalacturonan acetyl esterase (rgae) of the ce-12 family. as confirmation, this gene was cloned, and the product was expressed in escherichia coli strain rosetta (de3) cells and purified. the enzyme obtained was monomeric, with a molecular mass of 45 kda, and exhibited alkaliphilic properties. a study of the inhibition of the activity by some modulators confirmed that the catalytic triad for the esterase ac ... | 2008 | 18083818 |
| aspergillus uvarum sp. nov., an uniseriate black aspergillus species isolated from grapes in europe. | a novel species, aspergillus uvarum sp. nov., is described within aspergillus section nigri. this species can be distinguished from other black aspergilli based on internal transcribed spacers (its), beta-tubulin and calmodulin gene sequences, by aflp analysis and by extrolite profiles. aspergillus uvarum sp. nov. isolates produced secalonic acid, common to other aspergillus japonicus-related taxa, and geodin, erdin and dihydrogeodin, which are not produced by any other black aspergilli. none of ... | 2008 | 18398215 |
| synthesis of oligosaccharides derived from lactulose and pectinex ultra sp-l. | the beta-galactosidase activity of the commercial enzymatic preparation pectinex ultra sp-l derived from aspergillus aculeatus has been used to hydrolyze and transgalactosylate the prebiotic carbohydrate lactulose. during this reaction, new oligosaccharides derived from lactulose have been detected by high-performance anion-exchange chromatography with pulsed amperometric detection (hpaec-pad). the presence of the trisaccharide 6'-galactosyl-lactulose, the major compound formed, has been confirm ... | 2008 | 18412359 |
| lactic fermentation of cellobiose by a yeast strain displaying beta-glucosidase on the cell surface. | the aspergillus aculeatus beta-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. the recombinant beta-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the c-terminal half region of the alpha-agglutinin. the beta-glucosidase expression plasmids were integrated into the genome. three strong promoters of s. cerevisiae, the tdh3, pgk1, and pdc1 promoters, were use ... | 2008 | 18443785 |
| evidence for a blockwise distribution of acetyl groups onto homogalacturonans from a commercial sugar beet (beta vulgaris) pectin. | commercial acid-extracted sugar beet pectin was extensively hydrolysed using an endo-polygalacturonase (anpgi from aspergillus niger or anpgii from a. niger or fmpg from fusarium moniliforme) in combination with aspergillus aculeatus pectin methyl-esterase (aapme). the homogalacturonan-derived oligogalacturonates released were quantified by high-performance anion-exchange chromatography and their structure determined by mass spectrometry. the different endo-polygalacturonases exhibited variable ... | 2008 | 18448141 |
| two novel species of aspergillus section nigri from thai coffee beans. | two novel species of aspergillus section nigri from thai coffee beans are described as aspergillus aculeatinus sp. nov. and aspergillus sclerotiicarbonarius sp. nov. their taxonomic status was determined using a polyphasic taxonomic approach with phenotypic (morphology and extrolite profiles) and molecular (beta-tubulin, internal transcribed spacer and calmodulin gene sequences) characteristics. a. aculeatinus sp. nov. is a uniseriate species with a similar morphology to aspergillus aculeatus an ... | 2008 | 18599725 |
| characterization of an alpha-l-rhamnosidase from aspergillus kawachii and its gene. | an alpha-l-rhamnosidase was purified by fractionating a culture filtrate of aspergillus kawachii grown on l-rhamnose as the sole carbon source. the alpha-l-rhamnosidase had a molecular mass of 90 kda and a high degree of n-glycosylation of approximately 22%. the enzyme exhibited optimal activity at ph 4.0 and temperature of 50 degrees c. further, it was observed to be thermostable, and it retained more than 80% of its original activity following incubation at 60 degrees c for 1 h. its t (50) val ... | 2008 | 18633609 |
| improvement of aspergillus oryzae for hyperproduction of endoglucanase: expression cloning of cmc-1 gene of aspergillus aculeatus. | fi-carboxymethylcellulase (cmc1; family 12) is one of the endoglucanases of aspergillus aculeatus and consists of single polypeptide chain of 221 amino acids. the cmc1 gene was expressed in aspergillus oryzae niad300 (niad-) under promoter 8142. the plasmid pcmg14 carrying the cmc1 gene at psti site was used as a source of the gene (920 bp) and aspergillus oryzae was successfully transformed by the plasmid pnan-cmc1 (harboring cmc1 gene). the plasmid was integrated in aspergillus oryzae niad300 ... | 2008 | 18661108 |
| extraction of green labeled pectins and pectic oligosaccharides from plant byproducts. | green labeled pectins were extracted by an environmentally friendly way using proteases and cellulases being able to act on proteins and cellulose present in cell walls. pectins were isolated from different plant byproducts, i.e., chicory roots, citrus peel, cauliflower florets and leaves, endive, and sugar beet pulps. enzymatic extraction was performed at 50 degrees c for 4 h, in order to fulfill the conditions required for microbiological safety of extracted products. high methoxy (hm) pectins ... | 2008 | 18788816 |
| response surface methodology: synthesis of short chain fructooligosaccharides with a fructosyltransferase from aspergillus aculeatus. | a transferase was isolated, purified and characterised from aspergillus aculeatus. the enzyme exhibited a ph and temperature optima of 6.0 and 60 degrees c, respectively and under such conditions remained stable with no decrease in activity after 5h. the enzyme was purified 7.1 fold with a yield of 22.3% and specific activity of 486.1umg(-1) after dialysis, concentration with polyethyleneglycol (30%) and deae-sephacel chromatography. it was monomeric with a molecular mass of 85kda and k(m) and v ... | 2009 | 19028090 |
| gelation of high-methoxy pectin by enzymic de-esterification in the presence of calcium ions: a preliminary evaluation. | cohesive gels have been obtained by de-esterification of 1.0wt% high-methoxy citrus pectin (degree of esterification approximately 68%) in the presence of ca(2+) cations, using a commercial preparation (novoshape) of fungal methyl esterase cloned from aspergillus aculeatus. a convenient rate of network formation (gelation within approximately 30min) was achieved at an enzyme concentration of 0.2 peu/g pectin. at a ca(2+)-concentration of 40mm and incubation temperature of 20 degrees c, severe sy ... | 2009 | 19100969 |
| production of the aspergillus aculeatus endo-1,4-beta-mannanase in a. niger. | the beta-mannanase gene (man1) from aspergillus aculeatus mrc11624 (izuka) was patented for application in the coffee industry. for production of the enzyme, the gene was originally cloned and expressed in saccharomyces cerevisiae. however the level of production was found to be economically unfeasible. here we report a 13-fold increase in enzyme production through the successful expression of beta-mannanase of aspergillus aculeatus mrc11624 in aspergillus niger under control of the a. niger gly ... | 2009 | 19277742 |
| regulation of the display ratio of enzymes on the saccharomyces cerevisiae cell surface by the immunoglobulin g and cellulosomal enzyme binding domains. | we constructed a novel cell surface display system to control the ratio of target proteins on the saccharomyces cerevisiae cell surface, using two pairs of protein-protein interactions. one protein pair is the z domain of protein a derived from staphylococcus aureus and the fc domain of human immunoglobulin g. the other is the cohesin (coh) and dockerin (dock) from the cellulosome of clostridium cellulovorans. in this proposed displaying system, the scaffolding proteins (fusion proteins of z and ... | 2009 | 19411409 |
| development of a homologous transformation system for aspergillus aculeatus based on the sc gene encoding atp-sulfurylase. | a homologous transformation system was developed using the endogenous atp-sulfurylase gene, aasc, as a selectable marker in aspergillus aculeatus. spontaneous mutation was proved to be beneficial in isolating aasc-deficient mutants. molecular analysis of sc(+) transformants revealed that the frequency of single copy integration at atp-sulfurylase locus was more than 40%. | 2009 | 19420695 |
| heterologous expression and optimized production of an aspergillus aculeatus endo-1,4-beta-mannanase in yarrowia lipolytica. | the aspergillus aculeatus mrc11624 man1 gene, encoding an endo-beta-1,4-mannanase, was cloned and expressed in the promising heterologous enzyme producer, the ascomycetous yeast yarrowia lipolytica. both single- and multi-copy transformants were constructed, and the secretion of the enzyme was evaluated as an in-frame fusion with the lip2 secretion signal, as well as with its natural secretion signal. in shake-flask analysis, the highest volumetric enzyme activity (13,073 nkat/ml) and specific e ... | 2009 | 19507068 |
| d-lactic acid production from cellooligosaccharides and beta-glucan using l-ldh gene-deficient and endoglucanase-secreting lactobacillus plantarum. | in order to achieve direct fermentation of an optically pure d: -lactic acid from cellulosic materials, an endoglucanase from a clostridium thermocellum (cela)-secreting plasmid was introduced into an l: -lactate dehydrogenase gene (ldhl1)-deficient lactobacillus plantarum (ldhl1) bacterial strain. cela expression and its degradation of beta-glucan was confirmed by western blot analysis and enzyme assay, respectively. although the cela-secreting ldhl1 assimilated cellooligosaccharides up to cell ... | 2010 | 19597813 |
| restricted cell elongation in arabidopsis hypocotyls is associated with a reduced average pectin esterification level. | cell elongation is mainly limited by the extensibility of the cell wall. dicotyledonous primary (growing) cell walls contain cellulose, xyloglucan, pectin and proteins, but little is known about how each polymer class contributes to the cell wall mechanical properties that control extensibility. | 2007 | 17572910 |
| effect of cosolvents on the structural stability of endoglucanase from aspergillus aculeatus. | the effects of cosolvents such as sucrose, glycerol, and sorbitol on endoglucanase have been studied by activity, circular dichroic spectroscopy, fluorescence, and apparent thermal transition temperature measurements. the endoglucanase activity increased by 4-fold at 40% cosolvent concentration under optimum conditions. the endoglucanase lost 50% of its activity when exposed to 90 degrees c for more than 30 min (1 h). in the presence of cosolvents, it maintained its original activity and native ... | 2009 | 19813733 |
| aspergillusol a, an alpha-glucosidase inhibitor from the marine-derived fungus aspergillus aculeatus. | a new tyrosine-derived metabolite, aspergillusol a (4), was isolated on a gram scale, together with a methyl ester of 4-hydroxyphenylpyruvic acid oxime (5) and secalonic acid a, from the marine-derived fungus aspergillus aculeatus cri323-04. the tetraol in 4 was identified as erythritol by comparison of the 1h nmr spectrum of its benzoylated derivative with those of benzoylated erythritol (7) and d-threitol (8), as well as by cellulose-based chiral hplc analysis. aspergillusol a (4) selectively ... | 2009 | 19824618 |
| evaluation of enzyme mixtures in releasing fermentable sugars from pre-pulping extracts of mixed northeast hardwoods. | one near-term option to developing a forest product biorefinery is to derive pre-pulping extract from incoming wood chips before the main pulping step. the release of monomer sugars from a xylan-rich extract, creating a fermentable substrate is a prerequisite for utilization of pre-pulping extract for production of ethanol or other value-added products. this study examined the individual and mixture efficiencies of two hemicellulolytic microbial enzymes and two xylanase preparations in catalyzin ... | 2010 | 20084471 |
| gene cloning and characterization of a novel thermophilic esterase from fervidobacterium nodosum rt17-b1. | a bioinformatic screening of the genome of the thermophilic bacterium fervidobacterium nodosum rt17-b1 for esterhydrolyzing enzymes revealed a putative bacterial esterase (fne) encoded by fond_1301 with typical gdsl family motifs. to confirm its putative esterase function, the fne gene was cloned, functionally expressed in escherichia coli, and purified to homogeneity. recombinant fne exhibited the highest esterase activity of 14,000 u/mg with p-nitrophenyl acetate (pnpc(2)) as substrate. the ca ... | 2010 | 20383468 |
| construction of a beta-glucosidase expression system using the multistress-tolerant yeast issatchenkia orientalis. | we demonstrate the value of the thermotolerant yeast issatchenkia orientalis as a candidate microorganism for bioethanol production from lignocellulosic biomass with the goal of consolidated bioprocessing. the i. orientalis mf-121 strain is acid tolerant, ethanol tolerant, and thermotolerant, and is thus a multistress-tolerant yeast. to express heterologous proteins in i. orientalis, we constructed a transformation system for the mf-121 strain and then isolated the promoters of tdh1 and pgk1, tw ... | 2010 | 20467739 |
| complete saccharification of cellulose at high temperature using endocellulase and beta-glucosidase from pyrococcus sp. | we investigated a potential for glucose production from cellulose material using two kinds of hyperthermophilic enzymes, endo-cellulase (eg) and beta-glucosidase (bgl). two bgls from hyperthermophile pyrococcus furiosus and mesophile aspergillus aculeatus were compared for complete hydrolysis of cellulose with p. horikoshii endo-cellulase (egph). the combination reactions by each bgl enzyme and egph could produce only glucose without the other oligosaccharides from phosphoric acid swollen avicel ... | 2010 | 20519912 |
| hydrolysis of softwood by aspergillus mannanase: role of a carbohydrate-binding module. | endo beta-1,4-mannanases (beta-mannanases, ec 3.2.1.78), belonging to cazy gh5 and gh26 families, catalyze the hydrolysis of structurally different mannans. in this study, the mannanase encoding gene of aspergillus aculeatus vn was expressed in aspergillus niger d15#26 using pan 52-4 vector, under the control of pgpda promoter and ttrpc terminator. in order to improve the hydrolytic capacity of this gh5 on lignocellulosic substrate, the family 1 carbohydrate-binding module (cbm1) of aspergillus ... | 2010 | 20541570 |
| characterization of functional intermediates of endoglucanase from aspergillus aculeatus during urea and guanidine hydrochloride unfolding. | low concentrations of urea and guhcl (2 m) enhanced the activity of endoglucanase (ec 3.1.2.4) from aspergillus aculeatus by 2.3- and 1.9-fold, respectively. the k(m) values for controls, in the presence of 2 m urea and guhcl, were found to be 2.4 +/- 0.2 x 10(-8) mol l(-1), 1.4 +/- 0.2 x 10(-8) mol l(-1), and 1.6 +/- 0.2 x 10(-8) mol l(-1), respectively. the dissociation constant (kd) showed changes in the affinity of the enzyme for the substrate with increases in the kcat suggesting an increas ... | 2010 | 20627273 |
| involvement of tbl/duf231 proteins into cell wall biology. | through map-based cloning we determined trichome birefringence (tbr) to belong to a plant-specific, yet anonymous gene family with 46 members in arabidopsis thaliana. these genes all encode the domain of unknown function 231 (duf231). tbr and its homolog trichome birefringence-like3 (tbl3) are transcriptionally coordinated with cellulose synthase (cesa) genes, and loss of tbr or tbl3 results in decreased levels of crystalline secondary wall cellulose in trichomes and stems, respectively. loss of ... | 2010 | 20657172 |
| direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast kluyveromyces marxianus displaying cellulolytic enzymes. | to exploit cellulosic materials for fuel ethanol production, a microorganism capable of high temperature and simultaneous saccharification-fermentation has been required. however, a major drawback is the optimum temperature for the saccharification and fermentation. most ethanol-fermenting microbes have an optimum temperature for ethanol fermentation ranging between 28 degrees c and 37 degrees c, while the activity of cellulolytic enzymes is highest at around 50 degrees c and significantly decre ... | 2010 | 20676628 |
| noah's nectar: the proteome content of a glass of red wine. | combinatorial peptide ligand libraries (cplls) have been adopted for harvesting and identifying traces of proteins present in red wines. surprisingly, although it is stated that red wines are in general fined with egg albumin, for all italian wines investigated (in the areas around chiari and verona as well as in the chianti area) we find that the only fining agent used is bovine casein, just like in white wines. although the typical levels of casein found range between 45 to 85μg/l, in one case ... | 2010 | 20813213 |
| structural and biochemical studies elucidate the mechanism of rhamnogalacturonan lyase from aspergillus aculeatus. | we present here the first experimental evidence for bound substrate in the active site of a rhamnogalacturonan lyase belonging to family 4 of polysaccharide lyases, aspergillus aculeatus rhamnogalacturonan lyase (rgl4). rgl4 is involved in the degradation of rhamnogalacturonan-i, an important pectic plant cell wall polysaccharide. based on the previously determined wild-type structure, enzyme variants rgl4_h210a and rgl4_k150a have been produced and characterized both kinetically and structurall ... | 2010 | 20851126 |
| advantages of isothermal titration calorimetry for xylanase kinetics in comparison to chemical-reducing-end assays. | in lignocellulosic raw materials for biomass conversion, hemicelluloses constitute a substantial fraction, with xylan being the primary part. although many pretreatments reduce the amount or change the distribution of xylan, it is important to degrade residual xylan so as to improve the overall yield. typically, xylanase reaction rates are measured in stopped assays by chemical quantification of the reducing ends. with isothermal titration calorimetry (itc), the heat flow of the hydrolysis can b ... | 2010 | 21074510 |
| maximal release of highly bifidogenic soluble dietary fibers from industrial potato pulp by minimal enzymatic treatment. | potato pulp is a poorly utilized, high-volume co-processing product resulting from industrial potato starch manufacturing. potato pulp mainly consists of the tuber plant cell wall material and is particularly rich in pectin, notably galactan branched rhamnogalacturonan i type pectin which has previously been shown to exhibit promising properties as dietary fiber. the objective of this study was to solubilize dietary fibers from potato pulp by a one-step minimal treatment procedure and evaluate t ... | 2011 | 21253720 |
| glutamate production from ß-glucan using endoglucanase-secreting corynebacterium glutamicum. | we demonstrate glutamate production from ß-glucan using endoglucanase (eg)-expressing corynebacterium glutamicum. the signal sequence tora derived from escherichia coli k12, which belongs to the tat pathway, was suitable for secreting eg of clostridium thermocellum using c. glutamicum as a host. using the tora signal sequence, endoglucanase from clostridium cellulovorans 743b was successfully expressed, and the secreted eg produced 123 mg of reducing sugar from 5 g of ß-glucan at 30 °c for 72 h, ... | 2011 | 21305281 |
| aspergillus saccharolyticus sp. nov., a new black aspergillus species isolated in denmark. | a novel species, aspergillus saccharolyticus sp. nov., is described within aspergillus section nigri species. this species was isolated in denmark from treated hardwood. its taxonomic status was determined using a polyphasic taxonomic approach with phenotypic (morphology and extrolite profiles) and molecular (beta-tubulin, internal transcribed spacer and calmodulin gene sequences, and universally-primed pcr fingerprinting) characteristics. these features clearly distinguished this species from o ... | 2011 | 21335500 |
| itaconic acid derivatives and diketopiperazine from the marine-derived fungus aspergillus aculeatus cri322-03. | three metabolites, pre-aurantiamine (1), (-)-9-hydroxyhexylitaconic acid (4) and (-)-9-hydroxyhexylitaconic acid-4-methyl ester (5), together with two known compounds, paraherquamide e (6) and secalonic acid d (7), were isolated from the marine-derived fungus, aspergillus aculeatus. | 2011 | 21397285 |
| characterization of galactooligosaccharides derived from lactulose. | galactooligosaccharides are non-digestible carbohydrates with potential ability to modulate selectively the intestinal microbiota. in this work, a detailed characterization of oligosaccharides obtained by transgalactosylation reactions of the prebiotic lactulose, by using β-galactosidases of different fungal origin (aspergillus oryzae, aspergillus aculeatus and kluveromyces lactis), is reported. oligosaccharides of degree of polymerization (dp) up to 6 were detected and quantified by hplc-esi ms ... | 2011 | 21641605 |
| influence of ethylenediaminetetraacetic acid (edta) on the structural stability of endoglucanase from aspergillus aculeatus. | the effect of the chelating agent ethylenediaminetetraacetic acid (edta) on the structure and function of endoglucanase is studied. in the presence of 2 mm edta, endoglucanase showed an enhanced enzymatic activity of 1.5-fold compared to control. no further change in activity was observed with increase in the concentration of edta to 5 mm. the k(m) values for control and in the presence of edta are 0.060 and 0.044%, respectively, and k(cat) was 1.9 min(-1) in the presence of edta. the kinetic pa ... | 2011 | 21651310 |
| asperaculin a, a sesquiterpenoid from a marine-derived fungus, aspergillus aculeatus. | a novel sesquiterpenoid, asperaculin a, possessing a novel [5,5,5,6]fenestrane ring system, was isolated from the marine-derived fungus aspergillus aculeatus cri323-04. the structure of asperaculin a was established by analysis of spectroscopic data. the name aspergillane is proposed for the sesquiterpene skeleton in asperaculin a. | 2011 | 21667999 |
| use of psychrophilic xylanases provides insight into the xylanase functionality in bread making. | the bread improving potential of three psychrophilic xylanases from pseudoalteromonas haloplanktis tah3a (xph), flavobacterium sp. msy-2 (rxfh) and unknown bacterial origin (rxyn8) was compared to that of the mesophilic xylanases from bacillus subtilis (xbs) and aspergillus aculeatus (xaa). xph, rxfh and rxyn8 increased specific bread volumes up to 28%, 18% and 18%, respectively, while xbs and xaa gave increases of 23% and 12%, respectively. this could be related to their substrate hydrolysis be ... | 2011 | 21806059 |
| purification, crystallization and x-ray diffraction study of extracellular dermal glycoprotein from carrot and the inhibition complex that it forms with an endo-ß-glucanase from aspergillus aculeatus. | extracellular dermal glycoprotein (edgp) may play an important role in the plant defence system of the carrot (daucus carota) as it has inhibitory activity against endo-ß-glucanase produced by invading pathogens. here, edgp and the inhibition complex that it forms with fi-cmcase, a carboxyl methyl cellulase from aspergillus aculeatus, were successfully crystallized. the hexagonal crystal of edgp belonged to space group p6(2), with unit-cell parameters a = b = 130.4, c = 44.5 å, ? = 120°. the mon ... | 2011 | 21795806 |
| activity of three +¦-1,4-galactanases on small chromogenic substrates. | +¦-1,4-galactanases belong to glycoside hydrolase family gh 53 and degrade galactan and arabinogalactan side chains of the complex pectin network in plant cell walls. two fungal +¦-1,4-galactanases from aspergillus aculeatus, meripileus giganteus and one bacterial enzyme from bacillus licheniformis have been kinetically characterized using the chromogenic substrate analog 4-nitrophenyl +¦-1,4-d-thiogalactobioside synthesized by the thioglycoligase approach. values of k(cat)/k(m) for this substra ... | 2011 | 21696710 |
| construction of a recombinant trichoderma reesei strain expressing aspergillus aculeatus +¦-glucosidase 1 for efficient biomass conversion. | to develop a trichoderma reesei strain appropriate for the saccharification of pretreated cellulosic biomass, a recombinant t. reesei strain, x3ab1, was constructed that expressed an aspergillus aculeatus +¦-glucosidase 1 with high specific activity under the control of the xyn3 promoter. the culture supernatant from t. reesei x3ab1 grown on 1% avicel as a carbon source had 63- and 25-fold higher +¦-glucosidase activity against cellobiose compared to that of the parent strain pc-3-7 and that of ... | 2011 | 21830204 |
| direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing saccharomyces cerevisiae cells. | the cost of the lignocellulose-hydrolyzing enzymes used in the saccharification process of ethanol production from biomass accounts for a relatively high proportion of total processing costs. cell surface engineering technology has facilitated a reduction in these costs by integrating saccharification and fermentation processes into a recombinant microbe strain expressing heterologous enzymes on the cell surface. we constructed a recombinant saccharomyces cerevisiae that not only hydrolyzed hemi ... | 2011 | 21741417 |
| ethanol production from cellulosic materials using cellulase-expressing yeast. | we demonstrate direct ethanol fermentation from amorphous cellulose using cellulase-co-expressing yeast. endoglucanases (eg) and cellobiohydrolases (cbh) from trichoderma reesei, and beta-glucosidases (bgl) from aspergillus aculeatus were integrated into genomes of the yeast strain saccharomyces cerevisiae mt8-1. bgl was displayed on the yeast cell surface and both eg and cbh were secreted or displayed on the cell surface. all enzymes were successfully expressed on the cell surface or in culture ... | 2010 | 20349451 |
| acidic β-mannanase from penicillium pinophilum c1: cloning, characterization and assessment of its potential for animal feed application. | the β-mannanase gene, man5c1, was cloned from penicillium pinophilum c1, a strain isolated from the acidic wastewater of a tin mine in yunnan, china, and expressed in pichia pastoris. the sequence analysis displayed the gene consists of a 1221-bp open reading frame encoding a protein of 406 amino acids (man5c1). the deduced amino acid sequence of man5c1 showed the highest homology of 57.8% (identity) with a characterized β-mannanase from aspergillus aculeatus belonging to glycoside hydrolase fam ... | 2011 | 22036533 |
| agrobacterium tumefaciens-mediated transformation of aspergillus aculeatus for insertional mutagenesis. | abstract: agrobacterium tumefaciens-mediated transformation (amt) was applied to aspergillus aculeatus. transformants carrying the t-dna from a binary vector pbig2rhph2 were sufficiently mitotically stable to allow functional genomic analyses. the amt technique was optimized by altering the concentration of acetosyringone, the ratio and concentration of a. tumefaciens and a. aculeatus cells, the duration of co-cultivation, and the status of a. aculeatus cells when using conidia, protoplasts, o ... | 2011 | 22166586 |
| a novel endo-1,4-β-mannanase from bispora antennata with good adaptation and stability over a broad ph range. | an endo-β-1,4-mannanase encoding gene, man5, was cloned from bispora antennata cbs 126.38, which was isolated from a beech stump. the cdna of man5 consists of 1,299 base pairs and encodes a 432-amino-acid protein with a theoretical molecular mass of 46.6 kda. deduced man5 exhibited the highest amino acid sequence identity of 58% to a β-mannanase of glycoside hydrolase family 5 from aspergillus aculeatus. recombinant man5 was expressed in pichia pastoris and purified to electrophoretic homogeneit ... | 2012 | 22258646 |
| effect of mutations on the thermostability of aspergillus aculeatus β-1,4-galactanase. | new variants of β-1,4-galactanase from the mesophilic organism aspergillus aculeatus were designed using the structure of β-1,4-galactanase from the thermophile organism myceliophthora thermophila as a template. some of the variants were generated using propka 3.0, a validated pka prediction tool, to test its usefulness as an enzyme design tool. the propka designed variants were d182n and s185d/q188t, g104d/a156r. variants y295f and g306a were designed by a consensus approach, as a complementary ... | 2015 | 25941560 |
| a novel transcriptional regulator, clbr, controls the cellobiose- and cellulose-responsive induction of cellulase and xylanase genes regulated by two distinct signaling pathways in aspergillus aculeatus. | the cellobiose- and cellulose-responsive induction of the fiii-avicelase (cbhi), fii-carboxymethyl cellulase (cmc2), and fia-xylanase (xynia) genes is not regulated by xlnr in aspergillus aculeatus, which suggests that this fungus possesses an unknown cellulase gene-activating pathway. to identify the regulatory factors involved in this pathway, we constructed a random insertional mutagenesis library using agrobacterium tumefaciens-mediated transformation of a. aculeatus ncp2, which harbors a tr ... | 2013 | 22851016 |
| validation and application of hplc-esi-ms/ms method for the quantification of rbbr decolorization, a model for highly toxic molecules, using several fungi strains. | a novel analytical method using hplc-ms/ms operating in selected reaction monitoring (srm) for evaluation of fungi efficacy to decolorize remazol brilliant blue r (rbbr) dye solution was developed, validated and applied. the method shows high sensibility allowing the detection of 4.6 pm of rbbr. four fungal strains were tested in liquid medium, three strains of aspergillus (aspergillus aculeatus, aspergillus flavus and aspergillus fumigatus) and phanerochaete chrysosporium. all fungi were able t ... | 2012 | 22985849 |
| highly efficient synthesis of fructooligosaccharides by extracellular fructooligosaccharide-producing enzymes and immobilized cells of aspergillus aculeatus m105 and purification and biochemical characterization of a fructosyltransferase from the fungus. | in this work, aspergillus aculeatus m105 was obtained to produce high extracellular fructooligosaccharide-producing enzyme activity. the maximum yields of fructooligosaccharides produced by its extracellular enzymes and immobilized cells were 67.54 and 65.47% (w/w), respectively. a fructosyltransferase (ftase), aaft32a, was purified from m105. the optimal ph and temperature of aaft32a were ph 5.0-6.0 and 65 °c, respectively. the km, vmax, and kcat values for the transfructosylating activity of a ... | 2016 | 27492129 |
| soybean bio-refinery platform: enzymatic process for production of soy protein concentrate, soy protein isolate and fermentable sugar syrup. | soybean carbohydrate is often found to limit the use of protein in soy flour as food and animal feed due to its indigestibility to monogastric animal. in the current study, an enzymatic process was developed to produce not only soy protein concentrate and soy protein isolate without indigestible carbohydrate but also soluble reducing sugar as potential fermentation feedstock. for increasing protein content in the product and maximizing protein recovery, the process was optimized to include the f ... | 2016 | 27207010 |
| towards complete hydrolysis of soy flour carbohydrates by enzyme mixtures for protein enrichment: a modeling approach. | soy protein is a well-known nutritional supplement in proteinaceous food and animal feed. however, soybeans contain complex carbohydrate. selective carbohydrate removal by enzymes could increase the protein content and remove the indigestibility of soy products for inclusion in animal feed. complete hydrolysis of soy flour carbohydrates is challenging due to the presence of proteins and different types of non-structural polysaccharides. this study is designed to guide complex enzyme mixture requ ... | 2016 | 26992789 |
| identification and characterization of an acidic and acid-stable endoxyloglucanase from penicillium oxalicum. | xyloglucan is a major structural macromolecule of the primary cell wall of spermatophytes. the hydrolysis of xyloglucan by xyloglucanases may facilitate the hydrolysis of cellulose by cellulases, which is beneficial for bioethanol production. penicillium oxalicum has been employed for commercial cellulase production. in p. oxalicum, many genes and proteins related to the degradation of structural macromolecules of the plant cell wall have been found, but no gene encoding a xyloglucanase has been ... | 2016 | 26840178 |
| co-expression of endoxylanase and endoglucanase in scheffersomyces stipitis and its application in ethanol production. | scheffersomyces stipitis strain bcc15191 is considered as a biotechnologically valuable yeast for its ability to ferment glucose and xylose, the main sugar components in plant biomass, to ethanol. however, the wild strain lacks of endogenous cellulases and hemicellulases that limited biomass utilization. in order to improve biomass degrading ability of s. stipitis bcc15191, new integrative plasmids harboring constitutive tef1 promoter and codon-optimized zeocin or hygromycin antibiotic resistanc ... | 2015 | 26378014 |
| cloning and genomic organization of a rhamnogalacturonase gene from locally isolated strain of aspergillus niger. | the rhg gene encoding a rhamnogalacturonase was isolated from the novel strain a1 of aspergillus niger. it consists of an orf of 1.505 kb encoding a putative protein of 446 amino acids with a predicted molecular mass of 47 kda, belonging to the family 28 of glycosyl hydrolases. the nature and position of amino acids comprising the active site as well as the three-dimensional structure were well conserved between the a. niger ctm10548 and fungal rhamnogalacturonases. the coding region of the rhg ... | 2015 | 26142900 |
| expression and evaluation of enzymes required for the hydrolysis of galactomannan. | the cost-effective production of bioethanol from lignocellulose requires the complete conversion of plant biomass, which contains up to 30 % mannan. to ensure utilisation of galactomannan during consolidated bioprocessing, heterologous production of mannan-degrading enzymes in fungal hosts was explored. the aspergillus aculeatus endo-β-mannanase (man1) and talaromyces emersonii α-galactosidase (agal) genes were expressed in saccharomyces cerevisiae y294, and the aspergillus niger β-mannosidase ( ... | 2014 | 24888762 |
| identifying and characterizing the most significant β-glucosidase of the novel species aspergillus saccharolyticus. | the newly discovered fungal species aspergillus saccharolyticus was found to produce a culture broth rich in β-glucosidase activity. in this present work, the main β-glucosidase of a. saccharolyticus responsible for the efficient hydrolytic activity was identified, isolated, and characterized. ion exchange chromatography was used to fractionate the culture broth, yielding fractions with high β-glucosidase activity and only 1 visible band on an sds-page gel. mass spectrometry analysis of this ban ... | 2012 | 22906186 |
| a revised architecture of primary cell walls based on biomechanical changes induced by substrate-specific endoglucanases. | xyloglucan is widely believed to function as a tether between cellulose microfibrils in the primary cell wall, limiting cell enlargement by restricting the ability of microfibrils to separate laterally. to test the biomechanical predictions of this "tethered network" model, we assessed the ability of cucumber (cucumis sativus) hypocotyl walls to undergo creep (long-term, irreversible extension) in response to three family-12 endo-β-1,4-glucanases that can specifically hydrolyze xyloglucan, cellu ... | 2012 | 22362871 |
| development of a gin11/frt-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicelluloses. | bioethanol produced by the yeast saccharomyces cerevisiae is currently one of the most promising alternatives to conventional transport fuels. lignocellulosic hemicelluloses obtained after hydrothermal pretreatment are important feedstock for bioethanol production. however, hemicellulosic materials cannot be directly fermented by yeast: xylan backbone of hemicelluloses must first be hydrolyzed by heterologous hemicellulases to release xylose, and the yeast must then ferment xylose in the presenc ... | 2014 | 25306430 |
| heterologous expression of aspergillus terreus fructosyltransferase in kluyveromyces lactis. | fructo-oligosaccharides are prebiotic and hypocaloric sweeteners that are usually extracted from chicory. they can also be produced from sucrose using fructosyltransferases, but the only commercial enzyme suitable for this purpose is pectinex ultra, which is produced with aspergillus aculeatus. here we used the yeast kluyveromyces lactis to express a secreted recombinant fructosyltransferase from the inulin-producing fungus aspergillus terreus. a synthetic codon-optimised version of the putative ... | 2016 | 27084521 |
| an in vitro antibacterial and antifungal effects of cadmium(ii) complexes of hexamethyltetraazacyclotetradecadiene and isomers of its saturated analogue. | to evaluate the antibacterial and antifungal effects of cadmium(ii) complexes with hexamethyltetraazacyclotetradecadiene ligands. | 2014 | 25312179 |
| rhamnogalacturonan i modifying enzymes: an update. | rhamnogalacturonan i (rgi) modifying enzymes catalyse the degradation of the rgi backbone and encompass enzymes specific for either the α1,2-bond linking galacturonic acid to rhamnose or the α1,4-bond linking rhamnose to galacturonic acid in the rgi backbone. the first microbial enzyme found to be able to catalyse the degradation of the rgi backbone, an endo-hydrolase (ec 3.2.1.171) derived from aspergillus aculeatus, was discovered 25 years ago. today the group of rgi modifying enzymes encompas ... | 2016 | 26255130 |
| imaging of bacterial and fungal cells using fluorescent carbon dots prepared from carica papaya juice. | in this paper, we have described a simple hydrothermal method for preparation of fluorescent carbon dots (c-dots) using carica papaya juice as a precursor. the synthesized c-dots show emission peak at 461 nm with a quantum yield of 7.0 %. the biocompatible nature of c-dots was confirmed by a cytotoxicity assay on e. coli. the c-dots were used as fluorescent probes for imaging of bacterial (bacillus subtilis) and fungal (aspergillus aculeatus) cells and emitted green and red colors under differen ... | 2015 | 26123674 |
| optimisation of synergistic biomass-degrading enzyme systems for efficient rice straw hydrolysis using an experimental mixture design. | synergistic enzyme system for the hydrolysis of alkali-pretreated rice straw was optimised based on the synergy of crude fungal enzyme extracts with a commercial cellulase (celluclast™). among 13 enzyme extracts, the enzyme preparation from aspergillus aculeatus bcc 199 exhibited the highest level of synergy with celluclast™. this synergy was based on the complementary cellulolytic and hemicellulolytic activities of the bcc 199 enzyme extract. a mixture design was used to optimise the ternary en ... | 2012 | 22728789 |
| Study of influential factors on oligosaccharide formation by fructosyltransferase activity during stachyose hydrolysis by Pectinex ultra SP-L. | The influence of reaction conditions for oligosaccharide synthesis from stachyose using a commercial enzymatic preparation from Aspergillus aculeatus (Pectinex Ultra SP-L) was studied. Oligosaccharides were analyzed by gas chromatography with flame ionization detection (GC-FID) and matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS). Galactosyl-melibiose (DP(3)) was synthesized as a result of fructosidase activity, whereas fructosyl-stachyose (DP(5)) and ... | 2011 | 21882802 |
| definition and characterization of enzymes for maximal biocatalytic solubilization of prebiotic polysaccharides from potato pulp. | potato pulp is a high-volume co-processing product resulting from industrial potato starch manufacturing. potato pulp is particularly rich in pectin, notably galactan branched rhamnogalacturonan i polysaccharides, which are highly bifidogenic when solubilized. the objective of the present study was to characterize and compare four homogalacturonan degrading enzymes capable of catalyzing the required solubilization of these pectinaceous polysaccharides from potato pulp in a 1 min reaction. an add ... | 2011 | 22112514 |
| biochemical characterization and overexpression of an endo-rhamnogalacturonan lyase from penicillium chrysogenum. | rhamnogalacturonan lyase (pcrgl4a) was purified from the culture supernatant of penicillium chrysogenum 31b. pcrgl4a optimal activity occurred between ph 7-8 and at 40 °c. conserved domain search analysis identified pcrgl4a as a member of polysaccharide lyase family 4. pcrgl4a contains two conserved catalytic and four conserved substrate-binding residues as determined by x-ray crystallography of the aspergillus aculeatus rg lyase. recombinant pcrgl4a (rpcrgl4a) expressed in escherichia coli demo ... | 2015 | 25666014 |
| screening and identification of novel ochratoxin a-producing fungi from grapes. | ochratoxin a (ota) contamination has been established as a world-wide problem. in this study, the strains with the ability of ota production were screened by analyzing the green fluorescence of the isolates colonies from the grapes in zhenjiang with 365 nm uv light and confirmed by hplc with fluorescent detection (hplc-fld). the results showed that seven isolates acquired the characteristic of the fluorescence, of which only five showed the ability of ota production as confirmed by hplc-fld anal ... | 2016 | 27845758 |
| enhanced cell-surface display and secretory production of cellulolytic enzymes with saccharomyces cerevisiae sed1 signal peptide. | recombinant yeast strains displaying aheterologous cellulolytic enzymes on their cell surfaces using a glycosylphosphatidylinositol (gpi) anchoring system are a promising strategy for bioethanol production from lignocellulosic materials. a crucial step for cell wall localization of the enzymes is the intracellular transport of proteins in yeast cells. therefore, the addition of a highly efficient secretion signal sequence is important to increase the amount of the enzymes on the yeast cell surfa ... | 2016 | 27183011 |
| direct ethanol fermentation of the algal storage polysaccharide laminarin with an optimized combination of engineered yeasts. | laminarin is the algal storage glucan and represents up to 35% of the dry weight of brown macroalgae. in this study, a novel laminarinase, gly5m, was first found using focused proteome analysis of a laminarin-assimilating marine bacterium, saccharophagus degradans, and the encoding gene was isolated. a gly5m-displaying yeast strain was prepared with the cell surface display system using saccharomyces cerevisiae. it showed a laminarin-degrading activity on the cell surface and caused the dominant ... | 2016 | 27287535 |
| characterization of mechanisms underlying degradation of sclerotia of sclerotinia sclerotiorum by aspergillus aculeatus asp-4 using a combined qrt-pcr and proteomic approach. | the biological control agent aspergillus aculeatus asp-4 colonizes and degrades sclerotia of sclerotinia sclerotiorum resulting in reduced germination and disease caused by this important plant pathogen. molecular mechanisms of mycoparasites underlying colonization, degradation, and reduction of germination of sclerotia of this and other important plant pathogens remain poorly understood. | 2017 | 28859614 |
| does osmotic stress affect natural product expression in fungi? | the discovery of new natural products from fungi isolated from the marine environment has increased dramatically over the last few decades, leading to the identification of over 1000 new metabolites. however, most of the reported marine-derived species appear to be terrestrial in origin yet at the same time, facultatively halo- or osmotolerant. an unanswered question regarding the apparent chemical productivity of marine-derived fungi is whether the common practice of fermenting strains in seawa ... | 2017 | 28805714 |
| heterologously expressed aspergillus aculeatus β-glucosidase in saccharomyces cerevisiae is a cost-effective alternative to commercial supplementation of β-glucosidase in industrial ethanol production using trichoderma reesei cellulases. | trichoderma reesei is a filamentous organism that secretes enzymes capable of degrading cellulose to cellobiose. the culture supernatant of t. reesei, however, lacks sufficient activity to convert cellobiose to glucose using β-glucosidase (bgl1). in this study, we identified a bgl (cel3b) from t. reesei (trcel3b) and compared it with the active β-glucosidases from aspergillus aculeatus (aabgl1). aabgl1 showed higher stability and conversion of sugars to ethanol compared to trcel3b, and therefore ... | 2016 | 26073313 |
| [display cellulolytic enzymes on saccharomyces cerevisiae cell surface by using flo1p as an anchor protein for cellulosic ethanol production]. | in this study, we constructed a yeast consortium surface-display expression system by using flo1 as an anchor protein. endoglucanase ii (egii) and cellobiohydrolase ii (cbhii) from trichoderma reesei, and β3-glucosidase 1 (bgli) from aspergillus aculeatus were immobilized on saccharomyces cerevisiae y5. we constructed the cellulose-displaying expression yeast consortium (y5/fegii:y5/fcbhii:y5/fbgli = 1:1:1) and investigated the enzymatic ability and ethanol fermentation. the displayed cellulolyt ... | 2014 | 25720155 |
| the binding of zinc ions to emericella nidulans endo-β-1,4-galactanase is essential for crystal formation. | a novel emericella nidulans endo-β-1,4-galactanase (engal) demonstrates a strong capacity to generate high levels of very potent prebiotic oligosaccharides from potato pulp, a by-product of the agricultural potato-starch industry. engal belongs to glycoside hydrolase family 53 and shows high (72.5%) sequence identity to an endo-β-1,4-galactanase from aspergillus aculeatus. diffraction data extending to 2.0 å resolution were collected from a crystal of engal grown from conditions containing 0.2 m ... | 2013 | 23908026 |
| secalonic acid- f inhibited cell growth more effectively than 5-fluorouracil on hepatocellular carcinoma in vitro and in vivo. | hepatocellular carcinoma (hcc), one of the most common types of liver cancer, could be treated with 5-fluorouracil (5-fu). due to its side effects, 5-fu is more often used as the co-administration drug in clinical practice. secalonic acid-f (saf), isolated from a fungal strain identified in our lab as aspergillus aculeatus, showed potent biological activities. the goal of this study was to evaluate the inhibitory effects of saf on hepatocellular carcinoma and to compare it with that of 5-fu. res ... | 2017 | 28253713 |
| heterologous expression of aspergillus aculeatus endo-polygalacturonase in pichia pastoris by high cell density fermentation and its application in textile scouring. | removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. in this study, an endo-polygalacturonase (endopg) from aspergillus aculeatus produced in pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation. | 2017 | 28209146 |
| development of a cellulolytic saccharomyces cerevisiae strain with enhanced cellobiohydrolase activity. | consolidated bioprocessing (cbp) is a promising technology for lignocellulosic ethanol production, and the key is the engineering of a microorganism that can efficiently utilize cellulose. development of saccharomyces cerevisiae for cbp requires high level expression of cellulases, particularly cellobiohydrolases (cbh). in this study, to construct a cbp-enabling yeast with enhanced cbh activity, three cassettes containing constitutively expressed cbh-encoding genes (cbh1 from aspergillus aculeat ... | 2014 | 25164958 |
| structure and antitumor activity of the extracellular polysaccharides from aspergillus aculeatus via apoptosis and cell cycle arrest. | two extracellular polysaccharides, designated as wpa and wpb, were isolated from the fungus aspergillus aculeatus using q-sepharose fast flow and sephacryl s-300 column chromatography. wpa composed of mannose and galactose in a molar ratio of 3.9:1.0, and wpb mainly contained mannose. the molecular weight of wpa and wpb was about 28.1 kda and 21.0 kda, respectively. on the basis of methylation and nmr analysis, the possible main chain of wpa was [→5)-β-d-galf-(1 → 2,6)-α-d-manp(1→], and wpb was ... | 2016 | 27557923 |
| crystal structure and genetic modifications of fi-cmcase from aspergillus aculeatus f-50. | cellulose is the major component of the plant cell wall and the most abundant renewable biomass on earth, and its decomposition has proven to be very useful in many commercial applications. endo-1,4-β-d-glucanase (ec 3.2.1.4; endoglucanase), which catalyzes the random hydrolysis of 1,4-β-glycosidic bonds of the cellulose main chain to cleave cellulose into smaller fragments, is the key cellulolytic enzyme. an endoglucanase isolated from aspergillus aculeatus f-50 (fi-cmcase), which is classified ... | 2016 | 27470581 |