| nutritional quality and the presence of anti-nutritional factors in leaf protein concentrates (lpc). | leaf protein concentrates were prepared from 25 different plant species. the concentrates contained acceptable levels of all essential amino acids; except methionine which was limiting with chemical scores ranging between 13.5-96%. lpcs from sesbania, nebergrass, lufa and sweet pepper were lethal to the rats. per ranged between 0.28 for safflower lpc up to 1.95 for rape. positive nitrogen balance was obtained with diets based on lpcs with mean values ranging between 6.4 and 22.42 mg n per day. t ... | 1999 | 10719564 |
| synergistic interactions of a potyvirus and a phloem-limited crinivirus in sweet potato plants. | when infecting alone, sweet potato feathery mottle virus (spfmv, genus potyvirus) and sweet potato chlorotic stunt virus (spcsv, genus crinivirus) cause no or only mild symptoms (slight stunting and purpling), respectively, in the sweet potato (ipomoea batatas l. ). in the spfmv-resistant cv. tanzania, spfmv is also present at extremely low titers, though plants are systemically infected. however, infection with both viruses results in the development of sweet potato virus disease (spvd) charact ... | 2000 | 10725195 |
| chromosome mapping of the sweet potato little leaf phytoplasma reveals genome heterogeneity within the phytoplasmas. | to further understand the genomic diversity and genetic architecture of phytoplasmas, a physical and genetic map of the sweet potato little leaf (spll) strain v4 phytoplasma chromosome was determined. pfge was used to determine the size of the spll-v4 genome, which was estimated to be 622 kb. a physical map was prepared by two-dimensional reciprocal digestions using the restriction endonucleases bsshii, smal, eagl and i-ceul. sixteen cleavage sites were located on the map. southern hybridization ... | 2000 | 10784048 |
| comparisons of coat protein gene sequences show that east african isolates of sweet potato feathery mottle virus form a genetically distinct group. | sweet potato feathery mottle virus (spfmv, genus potyvirus) infects sweet potatoes (ipomoea batatas) worldwide, but no sequence data on isolates from africa are available. coat protein (cp) gene sequences from eight east african isolates from madagascar and different districts of uganda (the second biggest sweet potato producer in the world) and two west african isolates from nigeria and niger were determined. they were compared by phylogenetic analysis with the previously reported sequences of ... | 2000 | 10795523 |
| identification of mammalian-like purple acid phosphatases in a wide range of plants. | purple acid phosphatases (paps) comprise a family of binuclear metal-containing hydrolases, members of which have been isolated from plants, mammals and fungi. polypeptide chains differ in size (animal approximately 35kda, plant approximately 55kda) and exhibit low sequence homology between kingdoms but all residues involved in co-ordination of the metal ions are invariant. a search of genomic databases was undertaken using a sequence pattern which includes the conserved residues. several novel ... | 2000 | 10854785 |
| the plant vacuolar sorting receptor atelp is involved in transport of nh(2)-terminal propeptide-containing vacuolar proteins in arabidopsis thaliana. | many soluble plant vacuolar proteins are sorted away from secreted proteins into small vesicles at the trans-golgi network by transmembrane cargo receptors. cleavable vacuolar sorting signals include the nh(2)-terminal propeptide (ntpp) present in sweet potato sporamin (spo) and the cooh-terminal propeptide (ctpp) present in barley lectin (bl). these two proteins have been found to be transported by different mechanisms to the vacuole. we examined the ability of the vacuolar cargo receptor atelp ... | 2000 | 10871276 |
| [assessment of the impact of the biological rodenticide biorat in populations of rodents settled in several crops of the republic of costa rica]. | the impact of biological rodenticide biorat on populations of rodents settled in some crops was determined; these crops are highly infested with sigmodon hispidus, a very common vector in various central american countries. the trapping technique and the appraisal of damages allowed to calculate the index of infestation which ranged from 75 to 90 percent in three crops. twenty for to 36 kg of biorat were applied to 6 ha planted with cucumis sativus (cucumber), ipomoea batata (sweet potato) and a ... | 1999 | 10887586 |
| delayed activation of post-transcriptional gene silencing and de novo transgene methylation in plants with the coat protein gene of sweet potato feathery mottle potyvirus. | the relationship between post-transcriptional gene silencing (ptgs) and dna methylation was examined using nicotiana benthamiana transformed with the coat protein gene including the 3' non-translated region of sweet potato feathery mottle potyvirus. line 4.28 showed a delayed activation of the transgene silencing in comparison with the other silenced lines, and showed complete resistance against the recombinant potato virus x engineered to contain the sequence homologous to the transgene when th ... | 2000 | 10936519 |
| a complex containing both trypsin inhibitor and dehydroascorbate reductase activities isolated from mitochondria of etiolated mung bean (vigna radiata l. (wilczek) cv. tainan no. 5) seedlings. | a complex containing trypsin inhibitor (ti) activity was extracted with 0.1 m tris buffer (ph 7.9) from trypsin-treated mitochondria of etiolated mung bean seedlings, and further purified with a superdex 200 fplc column. this partially purified complex with an m(r) about 820 kda exhibited additional dehydroascorbate (dha) reductase activity with specific activities of 0.21, 1.53 and 1.54 mumol ascorbate formed min-1 mg-1 protein at ph 6.0, 6.5 and 7.0, respectively, when glutathione was added. m ... | 2000 | 10938863 |
| molecular characterization of tobacco mitochondrial l-galactono-gamma-lactone dehydrogenase and its expression in escherichia coli. | a cdna clone encoding l-galactono-gamma-lactone (gal) dehydrogenase (ec 1.3.2.3) was isolated from tobacco leaves. the cdna clone contained an open reading frame encoding the protein of 501 amino acids with a calculated molecular mass of 56,926 da, preceded by a putative mitochondrial targeting signal consisting of 86 amino acid residues. in fact, gal dehydrogenase was localized in the mitochondria of tobacco cells. the deduced amino acid sequence of the cdna showed 77 and 82% homology to caulif ... | 2000 | 10945335 |
| cytological and molecular evidence that the whitefly-transmitted cucumber vein yellowing virus is a tentative member of the family potyviridae. | cucumber vein yellowing virus (cvyv) is widespread in cucurbits in the middle east. cvyv has filamentous particles and is transmitted by bemisia tabaci by the semi-persistent mode. it has not yet been assigned to a specific genus or family. ultramicroscopic observations revealed numerous cylindrical cytoplasmic inclusions in melon and cucumber cells infected by cvyv isolates from israel and jordan. depending on the section orientation, the inclusions appeared as pinwheels or as bundles. in addit ... | 2000 | 10950987 |
| latin american food sources of carotenoids. | latin america has a wide variety of carotenogenic foods, notable for the diversity and high levels of carotenoids. a part of this natural wealth has been analyzed. carrot, red palm oil and some cultivars of squash and pumpkin are sources of both beta-carotene and alpha-carotene. beta-carotene is the principal carotenoid of the palm fruits burití, tucumã and bocaiuva, other fruits such as loquat, marolo and west indian cherry, and sweet potato. buriti also has high amounts of alpha-carotene and g ... | 1999 | 10971848 |
| detection and characterization of a 36-kda peptide in c-terminal region of a 24-kda vacuolar protein (vp24) precursor in anthocyanin-producing sweet potato cells in suspension culture. | a 24-kda vacuolar protein (vp24) was found to accumulate in anthocyanin-producing sweet potato cells (ipomoea batatas lam.) in suspension culture [nozue et al., plant physiol. 115 (1997) 1065]. vp24 cdna (accession no. ab025531) encodes a 96.3-kda large precursor protein with a c-terminal propeptide which contains the eight putative transmembrane domains. the mature vp24 is probably involved in the formation of intravacuolar pigmented globules (cyanoplasts) in highly anthocyanin-containing vacuo ... | 2000 | 11164584 |
| isolation of antidiabetic components from white-skinned sweet potato (ipomoea batatas l.). | we have already reported that white-skinned sweet potato (ipomoea batatas l.) (wssp) shows antidiabetic activity in streptozotocin (stz) induced diabetic rats and genetically diabetic models (yellow kk, db/db mice and zucker fatty rats). in this study, isolation and purification of the antidiabetic component of wssp were attempted. almost all antidiabetic activity was found in the cortex of wssp. the fractionation of the antidiabetic component in the wssp cortex was done by the following methods ... | 2001 | 11272813 |
| phase ii study of 4-ipomeanol, a naturally occurring alkylating furan, in patients with advanced hepatocellular carcinoma. | 4-ipomeanol (ipo; nsc 394438), a naturally occurring furan isolated from common sweet potatoes (ipomoea batatas) infected with the fungus fusarium solani was the first agent to be developed by the national cancer institute based on a biochemical-biological rationale as an anticancer agent targeted specifically against lung cancer. prior to clinical development, ipo was shown to induce pulmonary toxicity in the lungs of several mammalian species because the agent is metabolized to a highly reacti ... | 2001 | 11291834 |
| biotransformation of squamulosone by curvularia lunata atcc 12017. | squamulosone (aromadendr-1(10)-en-9-one), isolated in large quantity from the plant hyptis verticillata jacq. (labiatae), was incubated with the fungus curvularia lunata atcc 12017 in two different growth media. six metabolites were isolated from each medium. with five of the products being common to both fermentations. all seven metabolites are novel. the insecticidal activity of these aromadendranes was evaluated against the sweet potato weevil cylas formicarius elegantus. | 2001 | 11393517 |
| biosensors based on novel peroxidases with improved properties in direct and mediated electron transfer. | native horseradish peroxidase (hrp) on graphite has revealed approximately 50% of the active enzyme molecules to be in direct electron transfer (et) contact with the electrode surface. some novel plant peroxidases from tobacco, peanut and sweet potato were kinetically characterised on graphite in order to find promising candidates for biosensor applications and to understand the nature of the direct et in the case of plant peroxidases. from measurements of the mediated and mediatorless currents ... | 2000 | 11419644 |
| bovine atypical interstitial pneumonia associated with the ingestion of damaged sweet potatoes (ipomoea batatas) in northeastern brazil. | atypical interstitial pneumonia in cattle associated with the ingestion of damaged sweet potatoes is reported in the state of paraíba, northeastern brazil. the sweet potatoes were severely damaged by myzus tersicae, and had an obvious fungal infection. eighteen milking cows, 1 bull and 1 steer were fed approximately 400 kg of the sweet potatoes. six days after consumption 13 cows were affected with labored abdominal breathing, extended and lowered head, coughing, expiratory grunt, salivation and ... | 2001 | 11474732 |
| sugar-responsible elements in the promoter of a gene for beta-amylase of sweet potato. | expression of genes coding for sporamin and beta-amylase, the two most abundant proteins in storage roots of sweet potato, is coordinately inducible in atypical vegetative tissues by sugars. a sweet potato gene for beta-amylase (beta-amy) with introns as well as a beta-amy::gus fusion gene composed of the beta-amy promoter and the gus coding sequence, both showed sugar-inducible expression in leaves of transgenic tobacco which occurred via a hexokinase-independent pathway. analyses using various ... | 2001 | 11516155 |
| food production and nutrition in biosphere 2: results from the first mission september 1991 to september 1993. | the initial test of the biosphere 2 agricultural system was to provide a nutritionally adequate diet for eight crew members during a two year closure experiment, 1991-1993. the overall results of that trial are presented in this paper. the 2000 m2 cropping area provided about 80 percent of overall nutritional needs during the two years. adaptation of the crew to the diet which averaged 2200 calories, 73 g. of protein and 32 g. of fat per person over the course of the two years. the diet was ... | 1996 | 11538814 |
| zinc bioavailability in young adult rats fed sweet potato greens containing phytic acid. | this study determined whether phytic acid in sweet potato greens influences the bioavailability of zinc in young adult rats used as models for adult humans. a control diet (ain-93m), two ain-93m diets with pure phytic acid (pa) at 0.2% or 0.4%, and four diets with georgia jet or tu-82-155 dried blanched greens at 7.5% or 15% were fed to seven groups of 7-week male harlan-sprague rats for 21 days. weight gains were generally not affected by pa concentration, were lower in the rats fed with sweet ... | 1999 | 11542237 |
| food production and nutrition for the crew during the first 2-year closure of biosphere 2. | biosphere 2's finite natural resources: atmosphere, plants, water, and soil, and its unique increased rate of nutrient cycling, mandated a design for the agriculture that emphasized sustainability and high productivity. the results of the initial 2-year test of the agriculture system showed that it could provide a diet that was both nutritionally adequate and pleasing to the palate of the eight-member crew from september 1991 to september 1993. the agriculture design was developed from 1985 to 1 ... | 1997 | 11542292 |
| effect of nitrogen on resistance of sweet potato to sweetpotato weevil (coleoptera: curculionidae) and on storage root chemistry. | the effects of nitrogen fertilizer on sweet potato, ipomoea batatas (l.) poir., resistance to the sweetpotato weevil, cylas formicarius elegantulus (summers), was studied. adult weevil feeding and oviposition preference, larval survival, and pupal weight were used as measures of sweet potato resistance. sweet potato resin glycosides and caffeic acid concentrations in the periderm tissue of storage roots also were measured. sweet potato genotypes (beauregard, excel, w-244, w-250, and sumor) with ... | 2001 | 11681695 |
| energy expenditure in relation to activity of lesser mouse deer (tragulus javanicus). | heat production (hp) of male and female mouse deer during eating, standing and sitting was determined using the open circuit respiration chamber (rc). the time taken for similar activities was also determined in an outdoor enclosure (od). the animals were fed kangkong (ipomoea aquatica), sweet potato (ipomoea batatas) and rabbit pellet ad libitum. male mouse deer consumed more dry matter (dm), organic matter (om) and gross energy (ge) than female. the time for each activity of male and female mo ... | 2001 | 11691611 |
| [an epidemiological study on the risk factors of irritable bowel syndrome in zhanjiang, guangdong province]. | to assess the risk factors in the pathogenicity of irritable bouel syndrome(ibs) in zhanjiang city. | 2001 | 11851063 |
| wound-response regulation of the sweet potato sporamin gene promoter region. | sporamin, a tuberous storage protein of sweet potato, was systemically expressed in leaves and stems by wound stimulation. in an effort to demonstrate the regulatory mechanism of wound response on the sporamin gene, a 1.25 kb sporamin promoter was isolated for studying the wound-induced signal transduction. two wound response-like elements, a g box-like element and a gcc core-like sequence were found in this promoter. a construct containing the sporamin promoter fused to a beta-glucuronidase (gu ... | 2002 | 11855724 |
| direct absorption of acylated anthocyanin in purple-fleshed sweet potato into rats. | absorption of acylated anthocyanins in purple-fleshed sweet potato (ipomoea batatas cv. ayamurasaki) in rats was studied to obtain evidence that the acylated anthocyanins themselves could exert a physiological function in vivo. peonidin 3-caffeoylsophoroside-5-glucoside (pn 3-caf*sop-5-glc) in purple-fleshed sweet potato was directly absorbed into rat and present as an intact acylated form in plasma. after oral administration of the purple-fleshed sweet potato anthocyanin (psa) concentrate conta ... | 2002 | 11879056 |
| prevention by natural food anthocyanins, purple sweet potato color and red cabbage color, of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip)-associated colorectal carcinogenesis in rats initiated with 1,2-dimethylhydrazine. | the potential of purple sweet potato color (pspc) and red cabbage color (rcc), natural anthocyanin food colors, to modify colorectal carcinogenesis was investigated in male f344/ducrj rats, initially treated with 1,2-dimethylhydrazine (dmh) and receiving 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip) in the diet. after dmh initiation, pspc and rcc were given at a dietary level of 5.0% in combination with 0.02% phip until week 36. no pspc or rcc-treatment-related changes in clinical signs ... | 2002 | 11915369 |
| magnetite-alginate beads for purification of some starch degrading enzymes. | starch degrading enzymes, viz., beta-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. in each case, the enzyme activity was eluted by using 1.0 m maltose. beta-amylase (sweet potato), glucoamylase (aspergillus niger), and pullulanase (bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single ... | 2002 | 11936253 |
| spectral shift of ultraweak photon emission from sweet potato during a defense response. | consecutive spectral analyses of ultraweak photon emission from sweet potato showing a defense response were conducted to observe the process of physiological transition. the spectrum showed a drastic transition from 2 to 10 h after inoculation with fusarium oxysporum, during which the emission intensity increased slowly. the spectrum was stable from 10 to 36 h after inoculation, whereas the emission intensity peaked approximately 20 h after inoculation. a change in the physiological state conne ... | 2002 | 11950100 |
| in vitro fermentation properties of selected fructooligosaccharide-containing vegetables and in vivo colonic microbial populations are affected by the diets of healthy human infants. | salsify (tragopogon porrifolius) and gobo (arctium lappa) are root vegetables that contain high concentrations of naturally occurring fructooligosaccharides (fos), nondigestible oligosaccharides that have prebiotic effects on the intestinal microflora. the objective of this study was to compare colonic microbial populations and the fermentation characteristics of gobo and salsify in breast-fed vs. formula-fed infants. fecal inoculum from breast-fed and formula-fed infants consuming either no sol ... | 2002 | 12163660 |
| complete genome sequence and analyses of the subgenomic rnas of sweet potato chlorotic stunt virus reveal several new features for the genus crinivirus. | the complete nucleotide sequences of genomic rna1 (9,407 nucleotides [nt]) and rna2 (8,223 nt) of sweet potato chlorotic stunt virus (spcsv; genus crinivirus, family closteroviridae) were determined, revealing that spcsv possesses the second largest identified positive-strand single-stranded rna genome among plant viruses after citrus tristeza virus. rna1 contains two overlapping open reading frames (orfs) that encode the replication module, consisting of the putative papain-like cysteine protei ... | 2002 | 12186910 |
| synthesis, structure, properties, and phosphatase-like activity of the first heterodinuclear fe(iii)mn(ii) complex with the unsymmetric ligand h(2)bpbpmp as a model for the pap in sweet potato. | the new heterodinuclear mixed valence complex [fe(iii)mn(ii)(bpbpmp)(oac)(2)]clo(4) (1) with the unsymmetrical n(5)o(2) donor ligand 2-bis[((2-pyridylmethyl)-aminomethyl)-6-((2-hydroxybenzyl)(2-pyridylmethyl))-aminomethyl]-4-methylphenol (h(2)bpbpmp) has been synthesized and characterized. compound 1 crystallizes in the monoclinic system, space group p2(1)/c, and has an fe(iii)mn(ii)(mu-phenoxo)-bis(mu-carboxylato) core. two quasireversible electron transfers at -870 and +440 mv versus fc/fc(+) ... | 2002 | 12206683 |
| [the introduction of tobacco and the diffusion of smoking culture in korea]. | since its introduction to korea from japan at the beginning of the seventeenth century, tobacco became very popular with an amazing rapidity among koreans. along with widespread cultivation of tobacco, smoking also became very popular among koreans, regardless of their classes, ages, and sexes. on the other hand, other imported crops from america via europe in the sam period, like sweet potato, potato, corn and tomato, did not enjoy such popularity in korea. a long time after their introduction, ... | 2001 | 12219758 |
| sugar-induced increase of calcium-dependent protein kinases associated with the plasma membrane in leaf tissues of tobacco. | the sugar-inducible expression of genes for sporamin and [beta]-amylase in leaf explants of sweet potato (ipomoea batatas) and that of a [beta]-glucuronidase-fusion gene, with the promoter of the gene for [beta]-amylase in leaves of tobacco (nicotiana tabacum), requires ca2+ signaling (m. ohto, k. hayashi, m. isobe, k. nakamura [1995] plant j 7: 297-307), and it was inhibited by staurosporin and k252a, inhibitors of protein kinases. autophosphorylation activities of several potential protein kin ... | 1995 | 12228646 |
| inhibitors of protein phosphatases 1 and 2a block the sugar-inducible gene expression in plants. | genes coding for two major proteins of the tuberous root of sweet potato (ipomoea batatas), namely, sporamin and [beta]-amylase, are inducible in leaves and petioles when they are supplied with high concentrations of sucrose or other metabolizable sugars, such as glucose and fructose, and the accumulation of a large amount of starch accompanies this induction. three inhibitors of protein phosphatases 1 (pp1) and 2a (pp2a), namely, okadaic acid, microcystin-lr, and calyculin a, strongly inhibited ... | 1994 | 12232349 |
| analysis of genetic diversity in chinese sweetpotato [ipomoea batatas (l.) lam.] germplasm using dna amplification fingerprinting. | the genetic diversity and evolutionary relationships in a representative sample of chinese sweetpotato collection were assessed using the dna amplification fingerprinting approach. dna fingerprint profiles were developed for all 42 accessions tested. there were 19-26 bands for each accession and an average of 20.7 such bands were polymorphic. chinese sweetpotato germplasm tested exhibited a high degree of genetic diversity. phenetic analysis revealed five major clusters with the following compon ... | 1998 | 12296360 |
| after the 'affluent society': cost of living in the papua new guinea highlands according to time and energy expenditure-income. | what is the cost of living in the papua new guinea highlands? an answer is sought using a time and energy accounting approach. the subsistence regime of wola-speaking highlanders, the subjects of this investigation, comprises three components. the principal one is horticulture: people clearing gardens from forest and grassland, with tuberous crops predominating, notably sweet potato. the second component comprises animal rearing, notably of domestic pigs. the third, and least important, is hunti ... | 2002 | 12395862 |
| bioconversion of acid- and gamma-ray-treated sweet potato residue to microbial protein by mixed cultures. | sweet potato residue, a starchy agricultural waste, was used as a substrate to produce microbial protein by fusarium moniliforme and saccharomyces cerevisiae in submerged fermentation. acid- and gamma-irradiation-pretreated sweet potato residue enhanced the biomass yield and protein production when the residue was fermented with f. moniliforme and s. cerevisiae. a mixed culture of f. moniliforme and s. cerevisiae efficiently and rapidly utilized free sugars; the maximal biomass yield (13.96 g/l) ... | 2002 | 12407461 |
| anti-hyperglycemic effect of diacylated anthocyanin derived from ipomoea batatas cultivar ayamurasaki can be achieved through the alpha-glucosidase inhibitory action. | to clarify a postprandial glucose suppression effect of diacylated anthocyanin with alpha-glucosidase (agh) inhibitory activity, a single oral administration study of it in male 8-week-old sprague-dawley rats was performed. the diacylated anthocyanin used in this study was peonidin 3-o-[2-o-(6-o-e-feruloyl-beta-d-glucopyranosyl)-6-o-e-caffeoyl-beta-d-glucopyranoside]-5-o-beta-d-glucopyranoside isolated from storage roots of the purple sweet potato (ipomoea batatas cv. ayamurasaki), which showed ... | 2002 | 12452639 |
| antimutagenicity of mono-, di-, and tricaffeoylquinic acid derivatives isolated from sweetpotato (ipomoea batatas l.) leaf. | the caffeoylquinic acid derivatives, 3-mono-o-caffeoylquinic acid (chlorogenic acid, cha), 3,4-di-o-caffeoylquinic acid (3,4-dicqa), 3,5-di-o-caffeoylquinic acid (3,5-dicqa), 4,5-di-o-caffeoylquinic acid (4,5-dicqa) and 3,4,5-tri-o-caffeoylquinic acid (3,4,5-tricqa), and caffeic acid (ca) were isolated from the sweetpotato (ipomoea batatas l.) leaf. we examined the antimutagenicity of these caffeoylquinic acid compounds to promote new uses of the sweetpotato leaf. these caffeoylquinic acid deriv ... | 2002 | 12506969 |
| monoxenic in vitro production and colonization potential of am fungus glomus intraradices. | the paper reports the establishment of mycorrhizal infection of a non-mycorrhizal ri-t-dna transformed carrot root when co-cultured with a surface sterilized sweet potato root segment colonized by arbuscular mycorrhizal (am) fungus g. intraradices on minimal m medium. extensive fungal hyphal emergence from each cut end of the mycorrhizal sweet potato root piece was observed in one week old cultures. these hyphae caused infection on contacting the transformed-carrot- root segment and produced man ... | 2002 | 12587745 |
| expression and characterization of sweet potato invertase in pichia pastoris. | an invertase cdna (ibbetafruct1) was cloned from sweet potato leaves and characterized. the deduced amino acid sequence of the ibbetafruct1-encoded protein was closely related to vacuolar invertases and included the wecvd catalytic domain characteristic of them. an expression plasmid containing the coding region of ibbetafruct1 under the control of the alcohol oxidase promoter was used to transform the methylotrophic yeast pichia pastoris. the biochemical properties for the expressed recombinant ... | 2003 | 12590504 |
| sequence variability within the 3'-proximal part of the sweet potato mild mottle virus genome. | sweet potato mild mottle virus (spmmv) is the type member of the genus ipomovirus (family potyviridae) and is only known to occur in east africa. in uganda, spmmv is the third most prevalent virus infecting sweet potato. the sequence variability of spmmv was studied by cloning and sequencing a 1.8-kb fragment representing the 3'-end of the genome of eight spmmv isolates collected from different districts of uganda. sequence comparisons indicated 85.9-99.9% nucleotide sequence identity and 92.8-1 ... | 2003 | 12607100 |
| potential functional foods in the traditional maori diet. | the maori people were early new zealand settlers of polynesian descent. the incidence of non-infectious diseases appears to have been low in these people, perhaps in part due to the presence of protective chemical constituents within their food plant supply. three of the tropical crops they introduced are still eaten here today: the sweet potato or kumara (ipomoea batatas), the taro (colocasia esculenta) and the cabbage tree or ti (cordyline terminalis). sporamins a and b, the major storage prot ... | 2003 | 12628508 |
| candida kunwiensis sp. nov., a yeast associated with flowers and bumblebees. | a novel asexual ascomycetous yeast, candida kunwiensis (sg99-26t = kctc 17041t = cbs 9067t), was isolated from sweet potato (ipomoea batatas) flowers in korea and from the body surface of pollinating bumblebees in germany. comparative analysis of the d1/d2 domain of 26s rdna of all available sequences for ascomycetous yeasts showed that the novel species was phylogenetically related to the genus metschnikowia, but the sequence similarity was low. morphologically and physiologically, c. kunwiensi ... | 2003 | 12656196 |
| molecular biology and application of plant peroxidase genes. | peroxidases are a family of isozymes found in all plants; they are heme-containing monomeric glycoproteins that utilize either h(2)o(2) or o(2) to oxidize a wide variety of molecules. these important enzymes are used in enzyme immunoassays, diagnostic assays and industrial enzymatic reactions. peroxidase genes and their promoters can be used for molecular breeding of useful plants. transgenic techniques have also been used to investigate the physiological and molecular functions of peroxidase ge ... | 2003 | 12664144 |
| an elisa-based method for measurement of food-specific ige antibody in mouse serum: an alternative to the passive cutaneous anaphylaxis assay. | passive cutaneous anaphylaxis (pca) assay has been a gold standard method to measure allergen-specific ige antibody (asige ab) levels in allergy mouse models. many factors including stringent guidelines for laboratory animal use make pca a difficult choice. therefore, alternative methods are needed that can be readily applied for measurement of specific ige antibody levels in mouse serum. herein we describe a novel elisa-based method that is more sensitive in comparison to pca, ige isotype-speci ... | 2003 | 12667673 |
| food protein-induced enterocolitis syndrome caused by solid food proteins. | infantile food protein-induced enterocolitis syndrome (fpies) is a severe, cell-mediated gastrointestinal food hypersensitivity typically provoked by cow's milk or soy. solid foods are rarely considered a cause. | 2003 | 12671120 |
| interstrain inhibition in the sweet potato pathogen streptomyces ipomoeae: purification and characterization of a highly specific bacteriocin and cloning of its structural gene. | strains of the sweet potato soil rot pathogen streptomyces ipomoeae had previously been divided into three groups based on their ability to inhibit one another during pairwise cocultivation. while group i strains are not antagonistic to members of the other groups, group ii and group iii strains produce separate substances that are inhibitory to strains outside their respective cognate groups. here, we purified the group iii inhibitory substance from the culture supernatant of a representative s ... | 2003 | 12676701 |
| a novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells. | a strong oxidative stress-inducible peroxidase (pod) promoter was cloned from sweetpotato (ipomoea batatas) and characterized in transgenic tobacco plants and cultured cells in terms of environmental stress. a pod genomic clone (referred to as swpa2) consisted of 1824 bp of sequence upstream of the translation start site, two introns (743 bp and 97 bp), and a 1073 bp coding region. swpa2 had previously been found to encode an anionic pod which was highly expressed in response to oxidative stress ... | 2003 | 12777043 |
| sporamin-mediated resistance to beet cyst nematodes (heterodera schachtii schm.) is dependent on trypsin inhibitory activity in sugar beet (beta vulgaris l.) hairy roots. | sporamin, a sweet potato tuberous storage protein, is a kunitz-type trypsin inhibitor. its capability of conferring insect-resistance on transgenic tobacco and cauliflower has been confirmed. to test its potential as an anti-feedant for the beet cyst nematode (heterodera schachtii schm.), the sporamin gene spti-1 was introduced into sugar beet (beta vulgaris l.) by agrobacterium rhizogenes-mediated transformation. twelve different hairy root clones expressing sporamin were selected for studying ... | 2003 | 12777044 |
| expression and characterization of an anti-(hepatitis b surface antigen) glycosylated mouse antibody in transgenic tobacco (nicotiana tabacum) plants and its use in the immunopurification of its target antigen. | transgenic plants expressing recombinant immunoglobulins have arisen as an alternative technology for the large-scale production of antibodies useful in therapeutics and in industrial processes. in the present paper we report the expression in transgenic tobacco ( nicotiana tabacum ) of an anti-hbsag [anti-(hepatitis b virus surface antigen)] mouse igg1 mab (monoclonal antibody), currently used for the industrial purification of the recombinant vaccine antigen. using the sweet potato sporamin si ... | 2003 | 12797866 |
| identification of genes in the tomato big bud phytoplasma and comparison to those in sweet potato little leaf-v4 phytoplasma. | genetic relatedness of phytoplasmas is commonly defined on the basis of differences in the highly conserved 16s rrna gene, which may not resolve closely related phytoplasmas. an example of this is the closely related tomato big bud (tbb) and sweet potato little leaf strain v4 (spll-v4) phytoplasmas, which cannot easily be differentiated by their 16s rrna gene sequences. this study aimed to identify genes on the tbb phytoplasma chromosome which could be used to examine genetic variation between t ... | 2003 | 12855731 |
| development and transfer of genetically modified virus-resistant sweet potato for subsistence farmers in kenya. | up to 80% of the yield of sweet potato, the major root crop in east africa, can be destroyed by crop diseases such as the sweet potato feathery mottle virus (spfmv). a sweet potato that is resistant to spfmv is being developed through the combined efforts of non-governmental agenices, biotechnology groups, and agricultural entities. even small improvements in crop yield, owing to this new technology, will feed countless people in africa. | 2003 | 12908741 |
| production of galactinol from sucrose by plant enzymes. | galactinol, 1-o-(alpha-d-galactopyranosyl)-myo-inositol, was produced from sucrose as a starting material. udp-glc was prepared with sucrose and udp using sucrose synthase partially purified from sweet potato roots. then, the udp-glc was converted to udp-gal using yeast udp-gal 4-epimerase from a commercial source. finally, galactinol was produced from the udp-gal and myo-inositol using galactinol synthase partially purified from cucumber leaves. the product was identified as galactinol by the r ... | 2003 | 12913288 |
| coat protein sequence analysis reveals occurrence of new strains of sweet potato feathery mottle virus in uganda and tanzania. | the 3'-proximal part (1.8 kb) of the sweet potato feathery mottle virus (spfmv) genome was studied in four spfmv isolates collected from farmers' fields in western uganda (spfmv-bny), eastern uganda (spfmv-sor) and bagamoyo district, tanzania (spfmv-tz1 and spfmv-tz2). unlike the other three spfmv isolates, spfmv-sor was not detected with the polyclonal antisera to spfmv. it showed moderately high coat protein (cp) nucleotide (93.3-96.7%) and amino acid (93.6-96.8%) sequence identity to the isol ... | 2003 | 12913357 |
| further variability within the genus crinivirus, as revealed by determination of the complete rna genome sequence of cucurbit yellow stunting disorder virus. | the complete nucleotide (nt) sequences of genomic rnas 1 and 2 of cucurbit yellow stunting disorder virus (cysdv) were determined for the spanish isolate cysdv-allm. rna1 is 9123 nt long and contains at least five open reading frames (orfs). computer-assisted analyses identified papain-like protease, methyltransferase, rna helicase and rna-dependent rna polymerase domains in the first two orfs of rna1. this is the first study on the sequences of rna1 from cysdv. rna2 is 7976 nt long and contains ... | 2003 | 12917477 |
| potential chemopreventive properties of anthocyanin-rich aqueous extracts from in vitro produced tissue of sweetpotato (ipomoea batatas l.). | anthocyanin-rich aqueous extracts from cell suspension cultures of a high anthocyanin-producing sweetpotato pl (purple line) cell line grown under two different media conditions, mm (multiplication medium) and apm (high anthocyanin-producing medium) and from the cell line's donor tissue, field-grown storage root (sr) of sweetpotato, cv. ayamurasaki, were evaluated for antioxidative (dpph test), antimutagenic (salmonella/reversion assay; mutagen, trp-p-1), and antiproliferative (human promyelocyt ... | 2003 | 13129295 |
| utilization of sweet potato starch by rats and its effect on the digestion of dietary protein. | | 1955 | 13278779 |
| ceratocystis infection in sweet potato: its effect on proteins, isozymes, and acquired immunity. | changes take place in the protein and isozyme patterns of tissue adjacent to cut surfaces of sweet potato roots infected by the fungus ceratocystis fimbriata. chromatography and gel electrophoresis of extracts from sections cut at known distances from a plane of infection showed that inoculation with a pathogenic or nonpathogenic isolate produced similar changes in several proteins and enzymes. inoculation of a susceptible variety of sweet potato with the nonpathogenic isolate induced in a thin ... | 1964 | 14199715 |
| o-diphenol oxidases in sweet potato infected by the black rot fungus. | | 1965 | 14299590 |
| [study of ceratocystis fimbriata toxins determination from sweet potato by high performance thin-layer chromatography]. | the extract solution from sweet potato was washed with 5% na2co3 and followed with distilled water and dried with dehydrated sodium sulfate (na2so4). the ether extract was evaporated and the residue (crude sample) was dissolved in a small volume of chloroform-soluble solution and was spotted on a silica gel g thin-layer plate developed with different solvent systems. pure ipomeamarone and ipomeamaronol were used as standards. the resolution was the best in the solvent system of petroleum ether-e ... | 2003 | 14535108 |
| the ai project: historical and ecological contexts. | this paper aims to review a long-term research project exploring the chimpanzee mind within historical and ecological contexts. the ai project began in 1978 and was directly inspired by preceding ape-language studies conducted in western countries. however, in contrast with the latter, it has focused on the perceptual and cognitive capabilities of chimpanzees rather than communicative skills between humans and chimpanzees. in the original setting, a single chimpanzee faced a computer-controlled ... | 2003 | 14566577 |
| endophytic nifh gene diversity in african sweet potato. | a cultivation-independent approach was used to identify potentially nitrogen-fixing endophytes in seven sweet potato varieties collected in uganda and kenya. nitrogenase reductase genes (nifh) were amplified by pcr, and amplicons were cloned in escherichia coli. clones were grouped by restriction fragment length polymorphism analysis, and representative nifh genes were sequenced. the resulting sequences had high homologies to nitrogenase reductases from alpha-, beta-, and gamma-proteobacteria an ... | 2003 | 14608421 |
| antioxidant and memory enhancing effects of purple sweet potato anthocyanin and cordyceps mushroom extract. | the effects of purple sweet potato anthocyanin (spa) and cordyceps mushroom extract (cme) on lipid peroxidation, 1,1-diphenyl-2-picrylhydrazyl (dpph) radicals and cognitive deficits were examined. both spa and cme exhibited dpph radical scavenging activities with similar potency. in contrast, only spa was shown to effectively inhibit lipid peroxidation initiated by fe2+ and ascorbic acid in rat brain homogenates. furthermore, spa markedly enhanced cognitive performance, assessed by passive avoid ... | 2003 | 14609130 |
| change in the crystal packing of soybean beta-amylase mutants substituted at a few surface amino acid residues. | in spite of the high similarity of amino acid sequence and three-dimensional structure between soybean beta-amylase (sba) and sweet potato beta-amylase (spb), their quaternary structure is quite different, being a monomer in sba and a tetramer in spb. because most of the differences in amino acid sequences are found in the surface region, we tested the tetramerization of sba by examining mutations of residues located at the surface. we designed the sba tetramer using the spb tetramer structure a ... | 2003 | 14631070 |
| development of a pilot system for converting sweet potato starch into glucose syrup. | sweet potato has been chosen as one of nasa's crops to support human beings in future space missions. one of the possible uses is to make syrup that can be used as a general sweetener. in this work a simple engineering system for converting sweet potato starch into glucose syrup was studied on a laboratory scale. the system comprises the following main units: a blender, continuous stirred tank reactor (cstr), centrifugal and vacuum filters, deionization column and vacuum evaporator. the system w ... | 2003 | 14631997 |
| some molecular characteristics of three viruses from spvd-affected sweet potato plants in egypt. | sweet potato feathery mottle virus (spfmv, genus potyvirus, family potyviridae), sweet potato chlorotic stunt virus (spcsv, genus crinivirus, family closteroviridae) and sweet potato virus g (spvg) were detected in naturally infected sweet potato plants grown in the delta region in egypt. before this study, spvg was reported only from china. two isolates of spfmv and one isolate of spvg were characterized for the 3'-proximal genomic sequences. phylogenetic analyses indicated that the spfmv isola ... | 2003 | 14648298 |
| the ecology of huli subsistence agriculture. | a detailed study of the ecology of subsistence agriculture in the tari basin was conducted to investigate the stability of intensive agroecosystems in the highlands of papua new guinea. the highly intensive agricultural systems that have evolved in the wetland environments in the lowest parts of the basin were found to be extremely stable and capable of further intensification. both soil fertility and sweet potato yields showed no signs of degradation even though some areas had been under contin ... | 2002 | 14658826 |
| applying a nutribusiness approach to increase animal source food consumption in local communities. | animal source foods (asf) in the diets of schoolchildren are beneficial for supporting optimal physical and cognitive development. nevertheless, behavioral change and economic development are needed to increase and sustain adequate meat product consumption by schoolchildren in developing countries. a nutribusiness enterprise may be one way for local communities to promote economic development while increasing the availability of meat for children. this work evaluates the feasibility of a nutribu ... | 2003 | 14672307 |
| effect of fermentation on sweetpotato (ipomoea batatas) toxicity in mice. | unfortunate bovine fatalities occurring after ingestion of mold-damaged sweetpotatoes preclude the use of the culled tubers in livestock feed. in cattle, mold-damaged sweetpotatoes induce an acute respiratory distress syndrome resulting in asphyxiation. because of this potential toxicity and the general abundance of culled sweetpotatoes, the detoxification efficacy of ensiling was explored since it is an easy and economically viable technique often applied to preserve livestock feed. sweetpotato ... | 2004 | 14733525 |
| further characterization of 'sweet potato virus 2': a distinct species of the genus potyvirus. | an incompletely described potyvirus isolate from sweet potato in taiwan, referred to as 'sweet potato virus 2' (spv2), was further characterised. electron microscopy revealed that spv2 has filamentous particles of 850 nm in length and induces cytoplasmic cylindrical inclusions consisting of pinwheels and scrolls. the virus was mechanically transmitted to several species of the genera chenopodium, datura, nicotiana, and ipomoea. two biotypes of myzus persicae transmitted spv2 in a non-persistent ... | 2004 | 14745592 |
| sensory characterization of a ready-to-eat sweetpotato breakfast cereal by descriptive analysis. | the sweetpotato [ipomoea batatas (l.) lam], an important industry in the united states, has been selected as a candidate crop to be grown on future long-duration space missions by nasa. raw sweetpotato roots were processed into flour, which was used to formulate ready-to-eat breakfast cereal (rtebc). twelve trained panelists evaluated the sensory attributes of the extruded rtebc using descriptive analysis. the samples were significantly different (p<0.05) for all attributes. twelve perceived sen ... | 2003 | 14974490 |
| molecular cloning of a cytosolic ascorbate peroxidase cdna from cell cultures of sweet potato and its expression in response to stress. | a cdna encoding a cytosolic ascorbate peroxidase (apx), swapx1, was isolated from cell cultures of sweet potato ( ipomoea batatas) by cdna library screening, and its expression in the context of various environmental stresses was investigated. swapx1 contains an orf of 250 amino acids (27.5 kda) encoding a protein with a pi value of 5.32. the swapx1 orf does not code for a transit peptide, suggesting that the product is a cytosolic isoform. rna blot analysis showed that swapx1 gene is expressed ... | 2004 | 14986108 |
| molecular cloning and characterization of a cdna encoding asparaginyl endopeptidase from sweet potato (ipomoea batatas (l.) lam) senescent leaves. | asparaginyl endopeptidase is a cysteine endopeptidase that has strict substrate specificity toward the carboxyl side of asparagine residues, and is possibly involved in the post-translational processing of proproteins. in this report one full-length cdna, spae, was isolated from senescent leaves of sweet potato (ipomoea batatas (l.) lam). spae contained 1479 nucleotides (492 amino acids) in the open reading frame, and exhibited high amino acid sequence homologies (c. 61-68%) with asparaginyl end ... | 2004 | 14990624 |
| control strategies for sweet potato virus disease in africa. | sweet potato virus disease (spvd), caused by dual infection with the whitefly-borne sweet potato chlorotic stunt virus (spcsv) and the aphid-borne sweet potato feathery mottle virus (spfmv), is the most serious disease of sweet potato in africa. spvd has been known there since at least the 1940s, although it took several decades to elucidate its aetiology. it occurs throughout africa and is particularly prevalent in the great lakes region. production of sweet potato is largely by resource-poor f ... | 2004 | 15036842 |
| whitefly transmission of sweet potato viruses. | three genera of plant viruses, begomovirus (geminiviridae), crinivirus (closteroviridae) and ipomovirus (potyviridae), contain members that infect sweet potato (ipomoea batatas) and are transmitted by whiteflies. the begomoviruses, sweet potato leaf curl virus (splcv) and ipomoea leaf curl virus (ilcv), and the ipomovirus sweet potato mild mottle virus are transmitted by bemisia tabaci, the sweet potato whitefly. the crinivirus, sweet potato chlorotic stunt virus (spcsv), is transmitted by b. ta ... | 2004 | 15036843 |
| in vitro evaluation of physiological activity of vinegar produced from barley-, sweet potato-, and rice-shochu post-distillation slurry. | vinegar was produced from barley-, sweet potato-, and rice-shochu post-distillation slurry using jar fermentor within 19 hrs. all the vinegars showed radical-scavenging activity, angiotensin i converting enzyme (ace) inhibition and advanced glycation endproducts (age) inhibition in vitro. the radical-scavenging activity of the vinegar produced from sweet potato-shochu post-distillation slurry was higher than that of other two kinds of vinegar on the organic matter basis. the ace inhibitory activ ... | 2004 | 15056886 |
| effect of preozonation on improvement of settleability of solid in highly concentrated organic wastewater of japanese wheat and sweet potato spirit-distillery. | solid-liquid separation of the wastewater is very difficult because of high viscosity and high ss concentration. in this study, the effectiveness of preozonation on improving the settleability of the solids in wheat and sweet potato wastewaters was investigated using a bench-scale system and pilot treatment system respectively. results showed that solid-liquid separation in the wheat wastewater was greatly improved by the decanter in the system (ss reduction from 24100 mg/l to 100 mg/l). however ... | 2004 | 15137644 |
| vasorelaxation induced by common edible tropical plant extracts in isolated rat aorta and mesenteric vascular bed. | in this study, the vasodilatory actions of nine edible tropical plant extracts were investigated. ipomoea batatas (sweet potato leaf), piper betle (betel leaf), anacardium occidentale (cashew leaf), gynandropsis gynandra (maman leaf), carica papaya (papaya leaf), and mentha arvensis (mint leaf) extracts exhibited more than 50% relaxing effect on aortic ring preparations, while piper betle and cymbopogon citratus (lemongrass stalk) showed comparable vasorelaxation on isolated perfused mesenteric ... | 2004 | 15138017 |
| feeding and survival of the malaria vector anopheles gambiae on plants growing in kenya. | the propensity of the malaria vector mosquito anopheles gambiae giles (diptera: culicidae) to ingest sugars from various plants, and subsequent survival rates, were assessed with laboratory-reared males and females offered eight species of plants commonly cultivated and/or growing wild in western kenya. in cages (no-choice bioassay), mosquitoes given the opportunity to feed on castorbean (ricinus communis l.) had the longest survival times (mean and median survival time of 6.99 +/- 0.23 and 5.67 ... | 2004 | 15189235 |
| differential expression of 10 sweetpotato peroxidase genes in response to bacterial pathogen, pectobacterium chrysanthemi. | to understand the function of each peroxidase (pod, ec 1.11.1.7) in terms of biotic stress, changes in pod specific activity and expression of 10 pod genes were investigated in four cultivars of sweetpotato (ipomoea batatas) after infection with pectobacterium chrysanthemi. pod specific activity (units mg(-1) protein) increased from 16 h after inoculation (hai) in three varieties. pod activities of two cultivars, shinwhangmi and white star, reached a maximum level at 24 hai by about three times ... | 2004 | 15191750 |
| [bio-environmental effects and index of remediation of multi-heavy metals polluted red soils]. | a pot experiment was conducted to study the bio-environmental effects of lime and organic manure application on red soil and paddy soil derived from red sandstone and polluted by multi-heavy metals. the results indicated that liming decreased the content of soil bioavailable cu and pb extracted with 0.05 mol/l hcl, while applying hog manure increased that of cd. soil bioavailable heavy metals (hms) showed an apparent relationship with soil dissolved carbon. applying lime and organic manure has a ... | 2004 | 15202245 |
| active recombinant thioredoxin h protein with antioxidant activities from sweet potato (ipomoea batatas [l.] lam tainong 57) storage roots. | recombinant thioredoxin h (trx2) overproduced in escherichia coli (m15) was purified by ni2+-chelated affinity chromatography. the molecular mass of trx2 is approximately 1.4 kda as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. total antioxidant status, 1,1-diphenyl-2-picrylhydrazyl (dpph) staining, reducing power method, fe2+-chelating ability, ferric thiocyanate (ftc) method, and protection of calf thymus dna against hydroxyl radical-induced damage were studied. the ... | 2004 | 15264905 |
| absorption of acylated anthocyanins in rats and humans after ingesting an extract of ipomoea batatas purple sweet potato tuber. | we evaluated the absorbability of anthocyanins in humans and rats administered with a beverage prepared from an extract of the tuber of purple sweet potato (ipomoea batatas cultivar ayamurasaki), or with an anthocyanin concentrate. two major anthocyanin components, cyanidin 3-o-(2-o-(6-o-(e)-caffeoyl-beta-d-glucopyranosyl)-beta-d-glucopyranoside)-5-o-beta-d-glucopyranoside) and peonidin 3-o-(2-o-(6-o-(e)-caffeoyl-beta-d-glucopyranosyl)-beta-d-glucopyranoside)-5-o-beta-d-glucopyranoside), were de ... | 2004 | 15277754 |
| efficient developmental mis-targeting by the sporamin ntpp vacuolar signal to plastids in young leaves of sugarcane and arabidopsis. | plant vacuoles are multi-functional, developmentally varied and can occupy up to 90% of plant cells. the n-terminal propeptide (ntpp) of sweet potato sporamin and the c-terminal propeptide (ctpp) of tobacco chitinase have been developed as models to target some heterologous proteins to vacuoles but so far tested on only a few plant species, vacuole types and "payload" proteins. most studies have focused on lytic and protein-storage vacuoles, which may differ substantially from the sugar-storage ... | 2004 | 15372194 |
| potential chemopreventive properties of extract from baked sweet potato (ipomoea batatas lam. cv. koganesengan). | the extract from baked sweet potato (ipomoea batatas lam. cv. koganesengan) showed potential cancer-preventing effects. the extract was partially fractionated to four fractions (i, ii-a ii-b, and iii) by sephadex g-25 gel chromatography. the cytotoxicity against human myelocytic leukemia hl-60 cells, the suppression of tpa-induced transformation in mouse skin jb6 c141 cells, the apoptosis inducing activity in hl-60 cells, and the scavenging capacity against dpph radical were tested on the four f ... | 2004 | 15537331 |
| caffeoylsophorose, a new natural alpha-glucosidase inhibitor, from red vinegar by fermented purple-fleshed sweet potato. | the suppressive effect on the postprandial blood glucose rise through alpha-glucosidase (agh) inhibition was investigated in this study in order to clarify an antihyperglycemic function of 6-o-caffeoylsophorose (cs) from diacylated anthocyanin. the administration of cs (100 mg/kg) following maltose (2 g/kg) to sprague-dawley rats resulted in the maximal blood glucose level after 30 min being significantly decreased by 11.1% compared to the control. a reduction in the serum insulin secretion was ... | 2004 | 15564660 |
| phenolic composition and radical scavenging activity of sweetpotato-derived shochu distillery by-products treated with koji. | phenolic composition and radical scavenging activity in the shochu distillery by-products of sweetpotato (ipomoea batatas l.) treated with koji (aspergillus awamori mut.) and cellulase (cellulosin t2) were investigated to develop new uses. koji and cellulosin t2 treatment of shochu distillery by-products from sweetpotatoes, rice, and barley increased phenolic content. caffeic acid was identified as a dominant phenolic component in the shochu distillery by-products of the sweetpotato. adding koji ... | 2004 | 15618617 |
| phosphate forms an unusual tripodal complex with the fe-mn center of sweet potato purple acid phosphatase. | purple acid phosphatases (paps) are a family of binuclear metalloenzymes that catalyze the hydrolysis of phosphoric acid esters and anhydrides. a pap in sweet potato has a unique, strongly antiferromagnetically coupled fe(iii)-mn(ii) center and is distinguished from other paps by its increased catalytic efficiency for a range of activated and unactivated phosphate esters, its strict requirement for mn(ii), and the presence of a mu-oxo bridge at ph 4.90. this enzyme displays maximum catalytic eff ... | 2004 | 15625111 |
| effects of combination of caiapo with other plant-derived substance on anti-diabetic efficacy in kk-ay mice. | b. ludvik et al., have recently shown the effect of caiapo (ipomoea batatas l.) on reducing fasting blood glucose and insulin resistance in type-2 diabetic patients. it, however, was required 2-4 weeks after the single administration of caiapo. the present study aimed to determine if the combination therapy of caiapo with a mulberry leaf powder, which inhibits alpha-glucosidase, or with a loquat leaf extract, which shows an insulin-like effect, could make it possible to enhance the antidiabetic ... | 2004 | 15630271 |
| feeding of spinach or sweet-potato leaves and growth performance of growing pigs kept on smallholder farms in central vietnam. | we investigated whether the addition of either spinach or sweet-potato leaves to the diet of growing pigs, kept in small holdings in central vietnam, would improve growth performance. a control diet was formulated and mixed with each of the vegetables to a final concentration of 15% of the total dietary dry matter. the diets were fed to the pigs from 70 to 100 days of age on six different smallholder farms in central vietnam. there were three animals per treatment group per farm and biopsies of ... | 2004 | 15643817 |
| advancing human nutrition without degrading land resources through modeling cropping systems in the ethiopian highlands. | food shortage in sub-saharan africa is generally considered a function of limited access to food, with little thought to nutritional quality. analyzing household production of nutrients across farming systems could be valuable in guiding the improvement of those systems. an optimization model was employed to analyze the scenario of human nutrition and cropland allocation in enset (enset ventricosum)/root crop-based and cereal-based systems of the ethiopian highlands. the type and amount of nutri ... | 2004 | 15646312 |
| two cis-acting regulatory elements are involved in the sucrose-inducible expression of the sporamin gene promoter from sweet potato in transgenic tobacco. | in this study, we generated transgenic tobacco plants that express the beta-glucuronidase (gus) gene under the control of the 305-bp 5'-upstream region of a gene coding for sporamin a of sweet potato. expression of gus in excised tobacco leaves was induced by sucrose, mimicking the sugar-inducible expression of the endogenous sporamin genes in sweet potato. deletion of the sequences extending from position -305 (relative to the transcription start site) to -283 and from -146 to -87 resulted in a ... | 2005 | 15654621 |
| a regional food-frequency questionnaire for the us mississippi delta. | to describe food sources of nutrient intake for white and african american adults in the lower mississippi delta (lmd), and their use in the development of a regional food-frequency questionnaire (ffq) based on an earlier version of the national cancer institute's health habits and history questionnaire. | 2005 | 15705249 |
| utilization of ensiled sweet potato (ipomoea batatas (l.) lam.) leaves as a protein supplement in diets for growing pigs. | four diets were formulated with protein from fishmeal (fm), groundnut cake (gc), ensiled sweet potato (ipomoea batatas l. (lam.)) leaves (sp) or ensiled sweet potato leaves with lysine (spl). in experiment 1, 24 crossbred (large white x mong cai) growing pigs were allocated randomly by sex into four groups of six pigs and given one of four diets. experiment 2 was conducted using 16 crossbred pigs (large white x mong cai) at four farms. on each farm, pigs were allocated to two experimental groups ... | 2005 | 15729899 |
| [determination of furanoterpenoid toxins from sweet potato by thin-layer chromatography]. | sweet potato root tissue, when infected with different pathogens, invaded by insects, or irritated by certain chemicals, turns black-brown and produces and accumulates a large amount of furanoterpenoids such as ipomeamarone, ipomeamaronol, ipomeanine and 4-ipomeanol, which are toxic to animals. the furanoterpenoids from sweet potato root tissues infected with ceratocystis fimbriata were extracted with ether. the extract solution was washed with 5% na2co3 and followed with distilled water and dri ... | 1997 | 15739468 |
| effects on immune response of antidiabetic ingredients from white-skinned sweet potato (ipomoea batatas l.). | the present report describes the effects of antidiabetic ingredients from white-skinned sweet potato (awssp) on the immune response of human cells. | 2005 | 15797679 |
| the x-ray structure of a hemipteran ecdysone receptor ligand-binding domain: comparison with a lepidopteran ecdysone receptor ligand-binding domain and implications for insecticide design. | the ecdysone receptor is a hormone-dependent transcription factor that plays a central role in regulating the expression of vast networks of genes during development and reproduction in the phylum arthropoda. the functional receptor is a heterodimer of the two nuclear receptor proteins ecdysone receptor (ecr) and ultraspiracle protein. the receptor is the target of the environmentally friendly bisacylhydrazine insecticides, which are effective against lepidoptera but not against hemiptera or sev ... | 2005 | 15809296 |