| lectin binding to formalin-fixed paraffin sections of human endometrium. | the binding of fluorescein isothiocyanate conjugated concanavalin a (con a), triticum vulgaris (wga), glycine maximum (sba), dolichos biflorus (dba), ulex europaeus (uea), arachis hypogaea (pna), and ricinus communis (rca) to human proliferative, secretory, and atrophic endometrium was studied. the binding of con a and wga increased markedly during the progression of the endometrium from the proliferative to the secretory phase. triticum vulgaris seemed to bind to the secretion and was transport ... | 1987 | 3106250 |
| [separation of thomsen-friedenreich (t) antigen and n antigen precursor glycoproteins from perchloric acid-soluble fraction of cyst fluid of human ovarian clear cell carcinoma and their some chemical and serological properties]. | one m perchloric acid-soluble fraction separated from cyst fluid of a patient (blood group ab) with ovarian clear cell carcinoma in malignant was subjected to sephacryl s-200 gel filtration and fraction 1 which was eluted at void volume, reacted with both of the lectins of vicia unijuga and arachis hypogaea. this fraction was then separated into 6 fractions (deae-frs. 1-6) in a deae-sephacel column chromatography. deae-frs. 3-6 were sugar-rich glycoprotein fractions which contained galactose and ... | 1990 | 2165527 |
| detection of surface carbohydrates on pneumocystis carinii by fluorescein-conjugated lectins. | lectins react with a wide range of different carbohydrates (table 1). even so-called monospecific anti-h(o) lectins from lotus tetragonolobus, ulex europaeus, and anguilla anguilla react not only with the anti-h determinant but also with several fucosylated carbohydrates. consequently, the type of lectin receptor existing on the surface of pneumocystis carinii should be determined, because only a carbohydrate analysis can demonstrate the kind of carbohydrates which exist on the cell surface of t ... | 1987 | 3125541 |
| lectin binding to the human retina. | formalin-fixed, paraffin-embedded tissue sections of the human retina were stained with different fluorescein isothiocyanate-conjugated lectins. the lectins used were concanavalin a (con a), triticum vulgaris (wga), glycine maximum (sba), dolichos biflorus (dba), ulex europaeus (uea i), arachis hypogaea (pna), and ricinus communis (rca i). con a stained both the inner and outer segments of the rods and cones, whereas wga stained the inner and outer segments of the rods and the outer segments of ... | 1988 | 3128141 |
| pneumocystis carinii: surface reactive carbohydrates detected by lectin probes. | pneumocystis carinii obtained from rat lungs (rlh) and in vitro culture (rtc) were reacted with a panel of 14 fluorescein isothiocyanate conjugated lectins. percentage fluorescence and fluorescent intensity were determined for both trophic and cyst forms. all rlh and rtc derived organisms bound strongly concanavalin a (con a), and wheat germ agglutinin (wga). however, differences in soybean agglutinin (sba) binding between rlh and rtc organisms was observed. different subsets of the organism bou ... | 1988 | 3142787 |
| biochemical characterization of glycoprotein components in human ovarian cyst fluids by lectins. | 1. perchloric acid-soluble glycoprotein fractions (pasfs) were separated, in yields of 180-610 mg per 100 ml of cyst fluid, from the cyst fluids of human ovarian cystadenomas (ocas) in benign and borderline and ovarian clear cell carcinoma (occ) in malignant, and then identified as glycoproteins with 19.1-69.5% carbohydrate by their chemical composition analyses. 2. these pasfs reacted with anti-a, -b, -lea and/or -leb sera, but did not react with anti-h, -m and -n sera. all of the pasfs reacted ... | 1990 | 2165928 |
| application of flow cytometry to studies on the human acrosome. | this study describes the use of flow cytometry combined with specific labelling of the human sperm acrosome using a fitc-labelled plant lectin (arachis hypogea agglutinin). localization of the label to the acrosome was encouraged by freezing the sperm for at least 24 hours at -70 degrees c prior to labelling. studies of sperm from 53 normospermic men revealed that acrosome labelling followed a single normal distribution without the presence of subpopulations. the average fluorescence and degree ... | 1990 | 2211342 |
| the toxicity of indian calotropis procera rbr latex in the black rat, rattus rattus linn. | toxicity studies with latex of calotropis procera rbr were conducted in the black rat, rattus rattus. the latex was fed through bait prepared from wheat flour, ground nut oil and sugar at concentrations of 5, 7.5 or 10% (w/w). the bait was fed up to mortality and for a maximum of 10 days. the signs produced were passivity, sluggishness, sedation, dyspnea, weakness, reduction in weight, anorexia, diarhhea, hematuria, bleeding from nose, eyes and anus, eye lens opacity, mild tetanic convulsions, c ... | 1988 | 3176304 |
| glycoconjugate histochemistry in the glycocalyx of guinea pig middle ear mucosa. | an attempt was made to determine the composition of oligosaccharide side chains in the apical cell surface glycocalices of the middle ear epithelium. this glycoconjugate is located on the innermost luminal surface, but its function is still unknown. the lectins evaluated included triticum vulgare, canavalia ensiformis, maclura pomifera, arachis hypogaea, ricinus communis i (rca-i), and limax flavus. the staining results obtained with the six different lectin probes in the lowicryl k4m-embedded g ... | 1990 | 2393562 |
| specific and nonspecific inhibition of adhesion of oral actinomyces and streptococci to erythrocytes and polystyrene by caseinoglycopeptide derivatives. | various caseinoglycopeptide derivatives prepared from mammalian milk were evaluated as inhibitors of hemagglutinations mediated by actinomyces viscosus ny1, streptococcus sanguis omz9, and, for comparative purposes, plant lectins from arachis hypogaea and bauhinia purpurea. it was found that recognition of the beta-d-galactose-(1----3)-2-acetamido-2-deoxy-d-galactose carbohydrate chain by actinomyces viscosus ny1 organisms and arachis hypogaea and b. purpurea agglutinins had similar structural r ... | 1988 | 3182077 |
| isolation and characterization of amaranthin, a lectin present in the seeds of amaranthus caudatus, that recognizes the t- (or cryptic t)-antigen. | a lectin (amaranthin) present in the seeds of amaranthus caudatus has been isolated by fractionation on deae-cellulose followed by affinity chromatography on synsorb-t beads (gal beta 1,3galnac alpha-o-r-synsorb). the lectin appeared homogeneous by gel electrophoresis at ph 4.3 and gave a single protein band by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with mr = 33,000-36,000. a native mr = 54,000 was determined by gel filtration suggesting that amaranthin exists as a homodimer. ... | 1989 | 2777780 |
| mycoflora and mycotoxins of peanut (arachis hypogaea l.) seeds in egypt. iii. cellulose-decomposing and mycotoxin-producing fungi. | from 40 peanut seed samples collected in egypt, forty-three species and one variety of fungi, belonging to 16 genera, were collected. the most dominant genera were aspergillus (11 species + one variety), penicillium (11 species) and fusarium (4 species). from the preceding genera a. fumigatus, a. flavus, a. niger, p. chrysogenum and f. oxysporum were the most frequent species. forty-nine isolates belonging to 12 species and one variety were tested for production of mycotoxins, after growth on li ... | 1988 | 3216881 |
| lectin histochemistry of the embryonic heart: expression of terminal and penultimate galactose residues in developing rats and chicks. | rat embryos at days 10-18 of gestation and chicken embryos at days 3-6 of incubation were fixed and processed for lectin histochemistry. the distribution of binding sites for a lectin from the peanut arachis hypogaea (pna) conjugated to horseradish peroxidase (hrp) was determined on tissue sections both before and after enzymatic cleavage of sialic acid with neuraminidase (sialidase). endocardial cushion tissue in the rat, but not in the chick, reacted with pna-hrp prior to digestion with sialid ... | 1989 | 2916442 |
| lectin histochemistry on mucous substances of the taste buds and adjacent epithelia of different vertebrates. | in the present study carbohydrate residues in taste buds (tbs) and adjacent epithelial formations of a teleostean fish, a frog and the rabbit were detected by means of lectin histochemistry. biotinylated lectins from pisum sativum (psa), arachis hypogaea (pna), dolichos biflorus (dba), triticum vulgaris (wga and succinylated wga), glycine max (sba) and ulex europaeus (uea i) have been applied. the lectins were bound to an avidin-biotin-peroxidase-complex (abc) and visualized by diaminobenzidine/ ... | 1988 | 3259218 |
| carbohydrate-binding specificities of five lectins that bind to o-glycosyl-linked carbohydrate chains. quantitative analysis by frontal-affinity chromatography. | the carbohydrate-binding specificities of lectins purified from agaricus bisporus (aba-i), arachis hypogaea (pna), bauhinia purpurea (bpa), glycine max (sba), and vicia villosa (vva-b4) have been studied by affinity chromatography on columns of the immobilized lectins, and quantitatively analyzed by frontal affinity chromatography. these five lectins could be classified into two groups with respect to their reactivities with typical mucin-type glycopeptides, beta-d-galp-(1----3)-alpha-d-galpnac- ... | 1988 | 3274131 |
| early development of the representation of the body surface in si cortex barrel field in neonatal rats as demonstrated with peanut agglutinin binding: evidence for differential development within the rattunculus. | physiological studies have demonstrated a highly organized somatotopic representation of the body surface in si cortex of rat. this representation is correlated morphologically with the presence of barrel-shaped structures in layer iv. conventional staining techniques reveal barrels in the latter part of the first postnatal week. recently, the peroxidase conjugates of lectins, which recognize glycosylated molecules, have been used to study barrel field formation. con a, for example, has been sho ... | 1989 | 2792289 |
| properties of l-streptococci in comparison with those of a-streptococci. | despite some similarities, l-streptococci could be clearly differentiated from a-streptococci. formamide, autoclaved and nitrous acid extracts of all l-streptococcal cultures studied reacted only with their specific antisera and did not cross-react with any other group specific streptococcal antigens. all 33 l-streptococcal cultures, in contrast to a-streptococci, produced beta-d-glucuronidase and beta-d-galactosidase, hydrolyzed na-hippurate, grew on 10% and 40% bile blood agar and were aggluti ... | 1987 | 3299018 |
| organization of the boar spermatozoan plasma membrane: evidence for separate domains (subdomains) of integral membrane proteins in the plasma membrane overlying the principal segment of the acrosome. | indirect immunofluorescence microscopy and freeze-fracture have been used to identify overlapping subdomains at the peripheral rim of the sperm-head plasma membrane (pm) and the margin of the outer acrosomal membrane (oam) comprising the principal segment of the acrosome of the boar spermatozoon. an array of ridge-like structures (spaced 12-16 nm centre-to-centre), originally observed on the oam by aguas & pinto da silva, lies just beneath an area of the pm that is sparsely populated with large ... | 1987 | 3329199 |
| heterogeneity of apical glycoconjugates in kidney collecting ducts: further studies using simultaneous detection of lectin binding sites and immunocytochemical detection of key transport enzymes. | in the search for a functional role for the polarized glycoconjugates of rat collecting duct epithelial cells, the relation between binding of various lectins and expression of cellular transport enzyme profile of the cells was studied. for this purpose, principal and intercalated cells of rat kidney collecting duct were identified by morphological criteria and by their immunocytochemically determined content of (na+ + k+)-atpase and carbonic anhydrase (ca ii), respectively. various n-acetylgala ... | 1988 | 2853698 |
| uneventful administration of plasma products in a recipient with t-activated red cells. | a patient with t-polyagglutinable red cells and a severe coagulopathy provided an opportunity to observe the results of plasma transfusion in the face of t-activation. the patient was a 52-year-old navajo indian with a perforated gall bladder and related sepsis due to clostridium perfringens. the gall bladder was removed surgically. postoperatively, he had severe thrombocytopenia, and prolonged partial thromboplastin and prothrombin times. the patient's red cells were agglutinated by arachis hyp ... | 1986 | 2869600 |
| glycoconjugate expression in human renal oncocytomas. a lectin histochemical study. | a panel of lectins chosen for its specificity for segments of the nephron was used in an avidin-biotin-peroxidase reaction to stain 13 human renal oncocytomas and a specimen of oncocytomatosis. the lectins were derived from lotus tetragonolobus, glycine max, dolichos biflorus, and arachis hypogaea. two patterns predominated: one of staining with the lectins of d biflorus and g max, suggesting distal tubular or collecting-duct differentiation, and one of staining with the lectin of l tetragonolob ... | 1988 | 3395218 |
| specificity of fungal lipase in hydrolytic cleavage of oil. | lipase isolated from the fungus fusarium oxysporum f. sp. lini has shown specificity to saturated acids. in vitro hydrolytic splitting of cotton seed, ground-nut and fungal (f. oxysporum) oil substrates by the enzyme preparation from this fungus, revealed preferential fatty acid specificity. the enzyme attacked the triglyceride molecule, liberating 90-97% of saturated fatty acids, irrespective of length of the carbon chain. | 1987 | 3439435 |
| rapid lateral diffusion of lectin-labelled glycoconjugates in the human colonic adenocarcinoma cell line ht29. comparison with the synthetic lipid analogue dii-c14. | the lateral diffusion of lectin-labelled glycoconjugates was studied in the human colon carcinoma cell line ht29 using fluorescence photobleaching techniques. ht29 cells were grown in either dulbecco's modified eagle's medium with glucose (25 mm; dmem-glu) or with galactose (25 mm; dmem-gal). cell cultivation in the dmem-gal medium was assumed to promote a transformation of the cells to become small-intestinal-like with characteristic microvilli and associated enzymes. the diffusion of glycoconj ... | 1987 | 3484360 |
| method for showing human spermatogenesis using arachis hypogaea (aha) lectin. | using biotinylated arachis hypogaea agglutinin (aha) or peanut lectin (pna) and an avidin-peroxidase procedure, the various stages of spermatid development were visualised with great clarity; a light haematoxylin counterstain permitted the easy recognition of spermatogenic cells in the same section. this method is particularly useful in the interpretation of poorly fixed material and may be helpful in studies of cyclical maturation of spermatozoa, irrespective of whether the material is obtained ... | 1988 | 3056983 |
| evaluation of structural and secretory glycoconjugates in normal human jejunum by means of lectin histochemistry. | the labelling pattern of eight lectins was studied in jejunal samples from ten normal subjects, in order to define the normal distribution of structural and secretory glycoconjugates in the small bowel. the following lectins were studied by means of a peroxidase technique on formalin-fixed samples: arachis hypogaea, ricinus communis, canavalia ensiformis, lens culinaris, phaseolus vulgaris, triticum vulgaris, ulex europaeus, dolichos biflorus. phaseolus vulgaris reacted with goblet cell mucus th ... | 1987 | 3570880 |
| mycoflora and mycotoxins of peanut (arachis hypogaea l.) seeds in egypt. 1--sugar fungi and natural occurrence of mycotoxins. | sixty-four species and 2 varieties which belong to nineteen genera of fungi were identified from 40 peanut seed samples collected from different places in egypt by using a dilution-plate method on glucose-czapek's medium. the most frequent genera were aspergillus (21 species & 2 varieties), penicillium (16 species) and fusarium (6 species). a. flavus, a. fumigatus, a. niger, p. chrysogenum and f. oxysporum were the most common fungal species. forty seven percent of the samples proved to be toxic ... | 1987 | 3587340 |
| histogenesis of the renal cysts in adult (autosomal dominant) polycystic kidney disease: a histochemical study. | we studied the kidneys from ten patients with adult (autosomal dominant) polycystic kidney disease (apkd) stained with lectins specific for different segments of the nephron on 20 cysts from each case (ranging in size from 0.1 to 1.3 cm in nine cases and from 1.5 to 6 cm in one case). the epithelium of all cysts with positive reactivity (arachis hypogaea and epithelial membrane antigen) was of collecting duct origin. many cysts remained unstained. cysts of proximal tubule origin could not be ide ... | 1988 | 3065782 |
| identification of osteoclasts by rhodamine-conjugated peanut agglutinin. | rhodamine-conjugated arachis hypogaea (peanut agglutinin, pna) lectin, which is specific for beta-d-galactosyl and beta-d-gal-[1,3]-d-galnac residues, was used to identify osteoclasts in paraffin-embedded bone sections of fetal rat calvaria and a human bone-derived tumor, osteoclastoma. all multinucleated osteoclasts were positive for pna-lectin. this was also confirmed by studying smears of isolated osteoclasts from rat and chicken long bones. in addition to multinuclear cells, some mononuclear ... | 1986 | 3093027 |
| specific labelling by peanut agglutinin of the outer acrosomal membrane of the human spermatozoon. | experiments to bind fluorescein-conjugated arachis hypogea (peanut) agglutinin (fitc-pna) to washed human spermatozoa demonstrated that this lectin binds to the acrosome region in air-dried preparations. since there was no binding when labelling was performed in suspension, and comparable labelling to that seen in air-dried preparations was seen when spermatozoa treated with saponin (to lyse the plasma membrane) were labelled in suspension, the lectin must bind to an intracellular structure, pro ... | 1987 | 3118011 |
| cytochemical approach to the development and behaviour of rat alveolar type ii cells in culture. | the development and characteristics of rat alveolar type ii cells were monitored by using various cytochemical techniques. polarized light microscopy was found useful for observing live type ii cells in culture. cells progressively lose their birefringent granules starting from 48 h of cells in culture, indicating the disappearance of the phospholipids organized lamellae in the lamellar bodies. similar results were obtained by using an immunocytochemical approach with antibodies raised against t ... | 1988 | 2450083 |
| cell surface carbohydrate of leishmania mexicana amazonensis: differences between infective and non-infective forms. | the cell surface carbohydrates of leishmania mexicana amazonensis (amastigotes and promastigotes, both infective and non-infective forms) were comparatively analyzed by agglutination assay employing 28 highly purified lectins, and by binding assay using 125i-labeled lectins. among the d-galnac binding lectins, bandeiraea simplicifolia-i, dolichos biflorus, phaseolus vulgaris and glycine max were highly specific for the amastigotes, while that from maclura aurantiaca selectively agglutinated prom ... | 1986 | 3709546 |
| lectin reactivity in human large bowel. | the affinity of six lectins (arachis hypogaea, concanavalin a, bandeiraea simplicifolia i. dolichus biflorus, triticum vulgaris and lotus tetragonolobus) was studied in 10 normal human large intestines. the results showed that each lectin had its own characteristic staining pattern. they differed in their affinity for different cellular components, local distribution within crypts and regional distribution between right (ascending colon) and left (rectum) segments of the large bowel. arachis hyp ... | 1987 | 2451203 |
| fragmentation of the beta-subunit of human chorionic gonadotropin produced by choriocarcinoma. | when human chorionic gonadotropin (hcg) was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions with dithiothreitol (dtt), a smaller weight material (ctp'), in addition to the beta-subunit, could be detected by western blot analysis using antiserum for hcg beta-carboxy-terminal peptide (ctp). the ctp' band was much more apparent with urinary hcg from a patient with choriocarcinoma than with that from normal pregnant women. second-dimensional electroph ... | 1988 | 2454807 |
| purification and characterization of a differentiation-specific sialoglycoprotein of lactating-guinea-pig mammary tissue. | a large acidic glycoprotein, pas-i, was purified from the fat-globule membrane of guinea-pig milk. threonine and serine accounted for over 30 mol% of the amino acids, and galactose, n-acetylgalactosamine, n-acetylglucosamine, mannose and sialic acid were the principal sugars detected. on a molar basis, sialic acid accounted for over 60% of the total sugar. removal of sialic acid by treatment with neuraminidase revealed the presence of binding sites for peanut (arachis hypogaea) agglutinin, a lec ... | 1988 | 2456756 |
| histochemistry of glycosaminoglycans in cartilage ground substance. alcian-blue staining and lectin-binding affinities in semithin epon sections. | the critical-electrolyte-concentration staining method using alcian blue (ab) was applied to etched semithin epon-embedded sections. the distribution of various glycosaminoglycans (gags) was studied in hyaline, elastic, cellular and fibrous cartilage obtained from humans and rodents. the staining patterns in semithin sections were found to correspond to those obtained using paraffin-embedded material. lectin histochemistry was performed on consecutive sections. the following peroxidase-labelled ... | 1986 | 3744895 |
| cytochemical localization of carbohydrates in intercalated duct and acinar cells of mouse parotid gland. | the glycoconjugate composition of mouse intercalated duct and acinar cells of parotid gland has been compared. mucins containing 1,2-glycols were demonstrated by the tannic acid-uranyl acetate technique. hexose residues of glycoconjugates were identified using ferritin conjugated with canavalia ensiformis agglutinin (con a), triticum vulgare or wheat germ agglutinin (wga), ricinus communis i agglutinin (rca-i), phaseolus vulgaris agglutinin (pha-e) and arachis hypogaea agglutinin (pna). whereas ... | 1989 | 2470700 |
| neurotoxicity effects of some agglutinins extracted from two edible legumes--lima bean (phaseolus lunatus) and groundnut (arachis hypogea)--in growing albino rats. | | 1986 | 3751178 |
| isolation and partial characterization of the cell membrane of walker-256 carcinosarcoma ascites cells. | cell membranes of carcinosarcoma-256 ascites cells were isolated by the zn++ method. neutral sugars, sialic acid, fucose and n-acetylhexosamines were determined in the cell membrane fraction. cells were agglutinated by wheat germ agglutinin, concanavalin a, and the lectins from phaseolus vulgaris and glycine max. no agglutination was observed with peanut lectin (arachis hypogaea). gel electrophoresis under dissociating conditions revealed more than 40 bands in the membrane fraction after silver ... | 1989 | 2486452 |
| regional distribution of glycoconjugates in normal, transitional and neoplastic human colonic mucosa. a histochemical study using lectins. | regional distribution of glycoconjugates in normal and neoplastic colonic mucosa was studied by means of eight lectins: dolichos biflorus (dba), glycine max (sba), triticum vulgare (wga), arachis hypogaea (pna), griffonia simplicifolia-i (gs-i), canavalia ensiformis (con a), limax flavus (lfa), and ulex europaeus-i (uea-i). the lectin binding patterns were examined in 40 normal colonic mucosa (nm) (12 proximal (p) and 28 distal (d], 38 carcinomas (15 p and 23 d), and 31 transitional mucosa (tm) ... | 1989 | 2505446 |
| [some chemical and serological properties of non-dialysable fractions of human seminal plasmas]. | some chemical and serological properties of water-soluble and undialysable fraction (wsf) and water-insoluble and undialysable fraction (wisf) of human seminal plasmas were studied. both of the fractions contained proteins as the main components and also carbohydrates as minor components, and each fraction gave 9 bands which were stained with the periodate-schiff and/or coomassie brilliant blue reagents on sodium dodecyl sulfate-polyacrylamide slub gel electrophoresis. wsf reacted with 17 of 19 ... | 1989 | 2515272 |
| selective lectin reactions of two stocks of leishmania enriettii with differing pathogenicity. | five days old promastigote culture forms of two stocks of leishmania enriettii pathogenic and non-infective for cavia procellus, were tested with the lectins of canavalia ensiformis, ricinus communis-120, soja hispida (glycine maxima), arachis hypogaea, ulex europaeus, ulex europaeus i, ulex europaeus ii, laburnum alpinum, lotus tetragonolobus, euonymus europaeus and with a monoclonal antibody against blood group h. the pathogenic stock reacted with the anti-h lectin of lotus tetragonolobus and ... | 1987 | 3809146 |
| an immunofluorescent analysis of lectin binding to normal and regenerating skeletal muscle of rat. | ten different fluorescein-conjugated lectins of various known sugar specificities were used to study cell surface glycoconjugates of normal and regenerating rat skeletal muscle. in normal muscle, canavalia ensiformis agglutinin, triticum vulgaris agglutinin (wheat germ agglutinin, wga), ricinus communis agglutinin-i, and maclura pomifera agglutinin bound strongly to the endomysial region of the myofibers. no binding was observed in the cytoplasm of the myofibers. other lectins (dolichos biflorus ... | 1985 | 3915936 |
| o-glycosylation of leukosialin in k562 cells. evidence for initiation and elongation in early golgi compartments. | the o-glycosylation of leukosialin, a major sialoglycoprotein found on leukocytes, has been studied in the human erythroleukemic cell line k562. the appearance of its o-linked chains has been followed in pulse-chase experiments with [35s]methionine by immunoprecipitation with an anti-peptide antiserum as well as with a lectin from salvia sclarea seeds (ssa) specific for galnac-ser/thr and the peanut (arachis hypogaea) agglutinin (pna) which recognizes gal beta 1----3galnac-ser/thr structures. an ... | 1989 | 2526734 |
| [development of two assay systems of desialylated hcg specific for o-serine or n-asparagine linked carbohydrate chain and their clinical application]. | two specific and sensitive assays have been developed for the measurement of as-hcg in this study. these assay systems are immunoradiometric assays which utilize peanut lectin, arachis hypogaea (pna) or caster bean lectin, ricin communis agglutinin (rca-i), to selectively extract as-hcg and then directly quantify with purified and radiolabeled antiserum against hcg beta cooh terminal peptide (beta-ctp). pna is highly specific for the terminal carbohydrate linkage gal beta-(1----3)-galnac which i ... | 1985 | 3934006 |
| flow cytometric analysis of heterogeneity in blood group-related antigen expression in a human urinary bladder carcinoma cell line, 647v. | previous immunohistological studies showed a relationship between expression of blood group-related antigens (bg-ag) and invasive potential in human urinary bladder carcinoma, but the marked variability of antigen staining within many individual tumors has obscured the biological basis of this finding. we studied the expression of the a, h, and t (thomsen-friedenreich) bg-ag by flow cytometry in a human bladder carcinoma cell line (647v) using fluoresceinated bg-ag-specific lectins (dolichos bif ... | 1985 | 4005841 |
| characterization of a neuraminidase from corynebacterium aquaticum responsible for th polyagglutination. | th polyagglutinability is characterized by the agglutination of the red blood cells (rbc) by arachis hypogaea, medicago disciformis, vicia cretica but, in contrast to the t phenomenon, not by glycine max (glycine soja). because th transformation of rbc has been obtained in vitro, the mechanism of th polyagglutinability expression has been studied and reproduced experimentally. an enzyme with neuraminidase specificity has been isolated from the culture supernatant of corynebacterium aquaticum, an ... | 1989 | 2617954 |
| lectin binding affinities of induced pancreatic lesions in the hamster model. | we examined the binding pattern of nine lectins to n-nitrosobis(2-oxopropyl)amine (bop)-induced pancreatic lesions in syrian hamsters. these lectins were arachis hypogaea (pna), dolichos biflorus (dba), griffonia simplicifolia i (gs-i), helix aspersa (haa), helix pomatia (hpa), sophora japonica (sja), ricinus communis i (rca-i), triticum vulgaris (wga) and ulex europaeus i (uea-i). all of the lectins reacted in untreated control hamsters to varying intensities with cytoplasmic components of acin ... | 1985 | 4064253 |
| exposure of cryptantigens on red blood cell membranes in patients with acquired immune deficiency syndrome or aids-related complex. | patients with acquired immune deficiency syndrome (aids) or aids-related complex (arc) are subject to recurrent and severe infections due to organisms known to cause red blood cell membrane modifications. these red cell modifications include the exposure of novel carbohydrate cryptantigens that can react with naturally occurring antibodies and potentially result in hemolysis. we examined the frequency of cryptantigen exposure on the surface of red cells from aids/arc patients. blood samples from ... | 1989 | 2656988 |
| influence of some trace elements on the growth of rhizobium from peanut (arachis hypogaea l.) and gram (cicer arietinum l.). | | 1972 | 4679121 |
| endocarpic microorganisms of two types of windrow-dried peanut fruit (arachis hypogaea l.). | the endocarpic microorganisms of peanut fruit dried in either a random windrow (plants left as they fell from the digger) or an inverted windrow (plants inverted to expose fruit to sunlight) were different from that of freshly dug fruit. chaetomium, penicillium, trichoderma, rhizoctonia, and fusarium were the dominant fungi found associated with shells (pericarp) of freshly dug fruit. the dominant fungi of shells of windrowed fruit included chaetomium, rhizoctonia, fusarium, sclerotium, and alte ... | 1970 | 5466133 |
| a comparative study of hypocholesterolemic activity of lipid extract of bengal gram with safflower oil, mustard oil and ground-nut oil in albino rats. | | 1968 | 5711604 |
| aflatoxin-producing potential of isolates of the aspergillus flavus-oryzae group from peanuts (arachis hypogaea). | seventy-eight samples of farmer stock peanuts, representing peanuts grown in nine different geographical areas during 1964, were assayed for aflatoxin and examined for associated microflora. only two samples contained more than 50 ppb of aflatoxin. infestation by members of the aspergillus flavus-oryzae group varied from 35 to 100% of the kernels per area and from 1 to 100% of the kernels per sample. aflatoxin production by individual isolates ranged from 0 to 349,143 ppb under the test conditio ... | 1967 | 6031427 |
| nutritive value of groundnut (arachis hypogaea). 1. amino acid composition of different varieties of groundnut grown in the punjab. | | 1967 | 6052870 |
| nutritive value of groundnut (arachis hypogaea). 2. biological evaluation of different varieties of groundnut grown in the punjab. | | 1967 | 6052875 |
| studies on in vitro degradation and in vivo digestion of a slow ammonia releasing urea product. | a slow ammonia releasing urea product (uromalt) has been produced by autoclaving and drying of germinated barley and urea. in vitro degradability of nitrogen (ivdn, raab et al., 1983) was in average 62 +/- 9% in 4 hours, compared to 76 +/- 8% with an autoclaved and dried mixture of urea and not-germinated barley. mixtures of urea with leucaena l. or azadarichta indica (neem cake) showed degradabilities of about 80 and 93%, respectively, when dried at 39 degrees c, but of 65% when dried at 100 de ... | 1989 | 2735818 |
| lectin-dependent attachment of actinomyces naeslundii to receptors on epithelial cells. | the adherence of actinomyces naeslundii wvu45 to monolayer cultures of human epithelial cell lines was mediated by the lactose-sensitive fimbriae (type 2) of strain wvu45. the attachment of actinomyces viscosus t14v, which has both types 1 and 2 fimbriae, was approximately half that of a. naeslundii, and only minimal attachment of a. naeslundii and a. viscosus mutants lacking type 2 fimbriae was detected. the adherence of strain wvu45 was enhanced two- to threefold by neuraminidase treatment of ... | 1984 | 6150008 |
| isolation, characterization and implications of anti-tf (thomsen-friedenreich) agglutinins from different sources. | anti-tf agglutinins from peanut (arachis hypogaea) and from vertebrate sera of different species have been successfully isolated by affinity chromatography on acid-activated sepharose 4 b. the proteins were characterized by immunoelectrophoresis, polyacrylamide gel electrophoresis in the presence of sds and with respect to their carbohydrate binding specificities. anti-tf substances from sera showed one precipitin arc in immunoelectrophoresis, but quantitative immunoprecipitation revealed our hu ... | 1980 | 6157637 |
| expression of glycoconjugates on normally developing and immunologically impaired hymenolepis diminuta. | the carbohydrates on the surface of hymenolepis diminuta were analyzed with gold-labelled lectins, and it was found that the surface coat of the anterior body differs from that of the strobila in its lectin-binding properties. binding sites for lectins from abrus precatorius (apa), arachis hypogaea (pna), glycine max (sba) and for wheat germ agglutinin (wga) and succinylated wga were located on the scolex and strobilation zone. lectin-gold particles attached mainly to the electron-dense spines. ... | 1988 | 3237676 |
| cytochemical localization of small intestinal glycoconjugates by lectin histochemistry in controls and subjects with cystic fibrosis. | human mucosal glycoconjugates were examined in normal small intestinal biopsies from five control subjects using six different fluorescein-conjugated lectins: triticum vulgare agglutinin (wga), ulex europaeus agglutinin i (uea1), ricinus communis agglutinin i (rca1), glycin max-soy bean agglutinin (sba), dolichus biflorus agglutinin (dba), and arachis hypogaea peanut agglutinin (pna). these plant agglutinins bind to specific nonreducing end-terminal carbohydrate residues. only the lectins derive ... | 1983 | 6188589 |
| peanut agglutinin (pna) binding sites: a useful marker for hormonal dependence in experimental breast cancer. | histochemical studies were performed with the labeled lectin from arachis hypogaea (peanut agglutinin, pna) to detect the occurrence, distribution, and amount of this lectin receptor in n-nitrosomethylurea-induced rat mammary carcinomas. since in normal rat mammary tissue, the formation and secretion of pna binding sites are controlled by the combined action of estrogen and prolactin, the amount of secretion-associated pna binding sites was correlated with tumor response to either ovariectomy or ... | 1983 | 6309384 |
| site-specific monoclonal antibodies against peanut agglutinin (pna) from arachis hypogaea. immunohistochemical study of tissue-cultured cells and of 27 cases of hodgkin's disease. | the purpose of this study was to increase the sensitivity of the staining reaction for the t antigen on the surface of neoplastic cells grown in vitro with the use of site-specific monoclonal antibodies (mabs). the authors describe anti-peanut agglutinin (pna) mabs selected by screening the hybridomas with pna and pna bound to bovine serum albumin conjugated with the t antigen. the selected hybridomas (f2c8, f3d12, f3a5) were then grown in pristane-sensitized mice or in the amicon hollow fiber s ... | 1988 | 3282447 |
| differentiation of trypanosoma cruzi chagas, 1909 and trypanosoma vespertilionis battaglia, 1904 by various lectins. | four-days-old culture forms of trypanosoma cruzi (strain téhuantépéc, guatemala) and trypanosoma vespertilionis (strain p-14, p-9) were tested by 19 carbohydrate-specific agglutinins. the t. cruzi strains are interspecifically distinguishable with the lectins from euonymus europaeus, tridacna crocea, tridacna maxima and the human blood-group testserum anti-b from the t. vespertilionis strains. while the t. vespertilionis strains did react with anti-b and e. europaeus, the t. cruzi strains did no ... | 1983 | 6349060 |
| comparative study on the main membrane-surface sialoglycopeptides released from young and old human erythrocytes with trypsin. | 1. the amount of sialoglycopeptide (sgp) mixture released from young (y) erythrocytes by trypsin treatment was significantly larger than that from old (o) ones. 2. the two main membrane-surface sialoglycopeptides (y-sgp and o-sgp) were isolated in yields of about 45% and 42% from the mixtures by a combination of sephadex g-50 superfine gel filtration with deae-cellulose chromatography, respectively. molecular weights of 12,000 and 12,100, respectively, were found for the y-sgp and the o-sgp. 3. ... | 1988 | 3356136 |
| intraspecific characterization of t. cruzi stocks by the lectins from triticum vulgaris and arachis hypogaea. | the agglutination behavior of four-days-old epimastigote culture forms of t. cruzi, and t. cruzi-like stocks from different south american countries were tested with 15 lectins. two agglutination types can be distinguished: typ 1 includes stocks which react with triticum vulgaris and aaptos papillata ii (wga-type). type 2 includes stocks agglutinated by arachis hypogaea and aaptos papillata ii (pna-type). the reactions with the lectins from triticum vulgaris and aaptos papillata ii can be correl ... | 1984 | 6375213 |
| lectin receptors as markers of lymphoid cells. i. demonstration in tissue section by peroxidase technique. | the distribution of lectin binding receptors in human lymphoid tissue (lymph node and tonsil) was studied in an effort to identify lectins with potential application as cell markers. using a simple peroxidase technique applicable to formalin-fixed, paraffin-embedded tissue, the binding properties of five lectins were investigated: concanavalin a (con a), arachis hypogaea (peanut agglutinin), lotus tetragonolobus (asparagus pea), glycine max (soybean agglutinin) and bandeiraea simplicifolia. thre ... | 1984 | 6380299 |
| haemoglobin m-hyde park associated with polyagglutinable red blood cells in a south african family. | twelve of 35 members tested in a large ethnically-mixed south african family were found to have both haemoglobin m type hyde park and persistent polyagglutinable red blood cells. the characteristics of the polyagglutination have not been recorded previously. the cells of affected family members were not agglutinated by arachis hypogea, dolichos biflorus or salvia sclarea, but were agglutinated weakly by salvia horminum and bsii (gsii) and reacted strongly with glycine soja and sophora japonica l ... | 1988 | 3377987 |
| [inhibitory effect of lectins extracted from arachis hypogea and soybeans on germ cell migration in the embryo of xenopus laevis (amphibia, anura)]. | effect of injection of arachis hypogea (pna) and soybean (sba) lectins on the migration of xenopus primordial germ cells (pgcs) were investigated. pna inhibited almost entirely the pgcs migration, more than sba. our results indicated that the inhibition of the migration could be a consequence of the lectin-induced alterations of cell surface properties. | 1983 | 6412978 |
| cellular localization of lectin-affinity in tissue sections of normal human duodenum. | peroxidase (hrp) conjugates of arachis hypogaea agglutinin (pna), dolichos biflorus agglutinin (dba), ulex europaeus agglutinin i (uea i) and bandeiraea simplicifolia agglutinin i (bsa i) were used to detect the expression of carbohydrate moieties in glyco-conjugates of normal human duodenal mucosa. lectin histology was performed on sections from paraffin embedded specimens of donor blood groups a, b, ab and 0. while pna, dba and uea i staining appeared to be independent of the donor blood group ... | 1983 | 6419452 |
| lectin binding patterns in developing rat colon. | to evaluate qualitative changes in brush-border and goblet cell glycoconjugates during colonic development, rhodamine-conjugated lectins were applied to tissue from fetal (18-22 day), suckling (1-20 day), and weanling (22-26 day) rats. tissue from the proximal and distal colon of each animal was incubated with triticum vulgaris, arachis hypogaea, glycine max, or ulex europeus agglutinin i. formalin-fixed paraffin sections and unfixed frozen sections were coded, read blindly, and graded from nega ... | 1987 | 3557008 |
| glycoprotein allergens in pollen of timothy. v. significance of the carbohydrate moiety for the immunological activity of a basic glycoprotein allergen. | when the allergen was oxidized with periodate the size of its precipitate in rocket immunoelectrophoresis (rie) was reduced. incubation of the allergen with various glycosidases did not significantly affect its precipitation in rie. the binding of human ige and igg to allergen bound to nitrocellulose seemed not to be affected by incubation with alpha-l-fucosidase, beta-d-xylosidase, beta-d-galactosidase or periodate oxidation. incubation with alpha-d-mannosidase reduced the binding of igg to the ... | 1987 | 3596817 |
| histochemical characterization of glycoproteins present in jejunal and colonic goblet cells of pigs on different diets. a biopsy study using chemical methods and peroxidase-labelled lectins. | we examined the glycoprotein composition of intestinal goblet cells in jejunal and colonic biopsies obtained from pigs on different diets. paraffin sections were stained both chemically and with the following horseradish-peroxidase conjugated lectins: canavalia ensiformis (con-a), limulus polyphemus (lpa), lotus tetragonolobus (lta), arachis hypogaea (pna), ricinus communis (rca1), glycine max (sba) and triticum vulgaris (wga). using chemical staining procedures, only small quantitative differen ... | 1987 | 3624000 |
| a possible mechanism for the cellular coaggregation between actinomyces viscosus atcc 19246 and streptococcus sanguis atcc 10557. | the cells of actinomyces viscosus atcc 19246 (av19246) and streptococcus sanguis atcc 10557 (ss10557) coaggregated immediately after mixing in 40 mm-tris/hcl buffer. optimal conditions were ph 7.5 in the presence of ca2+ at 0.1 mm or higher. na2 edta and its analogues, na2mgedta and na2mnedta at 7.5 mm inhibited the coaggregation. trypsin and heat treatment impaired the reactive site on av19246 cells, but not on ss10557 cells. the coaggregates, once formed, dissociated gradually during extended ... | 1984 | 6481336 |
| identification of glycoprotein storage diseases by lectins: a new diagnostic method. | the specific diagnosis of glycoprotein storage diseases is made by demonstrating a deficiency in enzyme activity or an elevation of undegraded oligosaccharides in cells or body fluids. prospective sampling and expensive specialized biochemistry, which is also time consuming, are required for such studies. we used lectin reagents on paraffin-embedded tissue sections to identify the specific sugars in undegraded stored substances. we studied 22 cases of glycoprotein storage diseases and differenti ... | 1984 | 6501863 |
| flow cytometric analysis of the binding of eleven lectins to human t- and b-cells and to human t- and b-cell lines. | the relative surface binding of 11 lectins to human peripheral blood t- and b-lymphocytes, to molt-4 and jm t-cell lines, and to 6410 and nc37 b-cell lines was determined by flow cytometry. the lectins from lens culinaris (lca), ricinus communis (rca), arachis hypogaea (pna), abrus precatorius (apa), ulex europaeus (uea-f), sarothamnus scoparius (sas-f), helix pomatia (hpa), phaseolus coccineus (l-pha), glycine max (sba), and triticum vulgare (wga) were fluoresceinated and incubated with living, ... | 1984 | 6609053 |
| studies on the possible chemical, immunochemical and morphological differences at the cell surfaces of immunosensitive and immunoresistant, moloney virus-induced, lymphoma cell-lines. | comparison was made of several cell-surface parameters in the immunosensitive, moloney virus-induced, mouse lymphoma, yac, and its immunoresistant variant, yacir. the characteristics of the two cell lines appeared to be similar by most of the criteria employed. the poly(acrylamide)-gel electrophoresis (with sodium dodecyl sulfate) patterns, after staining with coomassie brilliant blue, of detergent-solubilized materials, appeared to be identical. after elution from a gel filtration column, no ma ... | 1983 | 6627250 |
| hemolytic-uremic syndrome and t-cryptantigen exposure by neuraminidase-producing pneumococci: an emerging problem? | hemolytic anemia, thrombocytopenia, and renal failure form a constellation of complications observed in patients infected with neuraminidase-producing pneumococci. the circulating enzyme causes exposure of the t-cryptantigen on cell surfaces to which most people possess a naturally occurring antibody. antigen-antibody interaction activates effector systems that lead to the clinical manifestations. the syndrome is most frequently seen in infants, in whom it carries more than a 50% mortality rate. ... | 1983 | 6687290 |
| energy utilization of supplemented cereal diets in human volunteers. | energy utilization was studied in human volunteers using different diets containing wheat flour supplemented by groundnut (arachis hypogaea), "masur" (lens culinaris), mung (phaseolus aureus) and gram (cicer arietinum) flour. digestible and metabolizable energies were determined for all the experimental diets. an improved energy digestibility was observed when wheat flour was supplemented with groundnut flour, and groundnut flour plus gram flour, i.e. 93.35 and 89.48%, respectively. percent dige ... | 1986 | 3632213 |
| lectins as markers of human epidermal cell differentiation. | the expression of sugar residues on human epidermal cells was investigated by means of lectin binding, as a way of determining membrane structural changes occurring during the differentiation of the epidermis. fourteen lectins of different sugar specificity were conjugated with fluorescein isothiocyanate (fitc-lectins) and tested in fluorescence microscopy on frozen sections of normal human epidermis. in parallel, fitc-lectins were tested on psoriatic-involved epidermis to visualize differences ... | 1982 | 6816653 |
| high-mr glycoprotein profiles in human milk serum and fat-globule membrane. | gradient-polyacrylamide-gel electrophoresis of human milk serum separated three high-mr glycoprotein bands. the properties of the components corresponding to the three bands (tentatively termed 'components c, b and a' in their order of migration) were compared by staining with four monoclonal antibodies and lectins. components b and c both reacted with the four antibodies, but component a did not. components b and c were stained with peanut (arachis hypogaea) agglutinin (pna) and wheat (triticum ... | 1986 | 3707520 |
| production of bacillus sphaericus strain 1593 primary powder on media made from locally obtainable nigerian agricultural products. | five media, formulated from dried cow blood, mineral salts, and seeds from four species of legumes, were assessed for growth, sporulation, and insecticidal properties of bacillus sphaericus strain 1593. bacterial powders, prepared from broth, were assayed against culex quinquefasciatus, anopheles gambiae, and aedes aegypti. good growth and sporulation were obtained with all the media. the highest number of viable cells and spores per mililitre (8.6 x 10(8) and 8.1 x 10(8] were obtained in media ... | 1983 | 6883226 |
| alterations in human colonic mucin occurring with cellular differentiation and malignant transformation. | the binding of fluorescein isothiocyanate (fitc)-conjugated lectins to mucin in the human colon was studied by using fluorescence microscopy. in normal mucosa, lectins that preferentially bind to exposed n-acetyl-galactosamine residues (dolichos biflorus agglutinin and soybean agglutinin) bound selectively to the goblet cell mucin of well-differentiated cells in the upper colonic crypt. by contrast, lectins that require exposed non-reducing galactose residues for binding (ricinus communis agglut ... | 1982 | 6952252 |
| the major sialoglycoprotein of human t-lymphocytes. | human, peripheral-blood t-lymphocytes and human, t-lymphoblastoid cells of a molt 4b cell-line were surface-labeled by lactoperoxidase-catalyzed iodination, periodate and sodium borotritide, and galactose oxidase and sodium borotritide, and analyzed by dodecyl sodium sulfate-polyacrylamide gel-electrophoresis. both types of cells were found to show a major, cell-surface sialoglycoprotein with an apparent mol. wt. of 95,000. after neuraminidase treatment, this glycoprotein showed a higher mol. wt ... | 1980 | 6965465 |
| surface carbohydrate changes on onchocerca lienalis larvae as they develop from microfilariae to the infective third-stage in simulium ornatum. | use was made of seven fitc labelled lectins as tools to investigate the surface of onchocerca lienalis larvae as they develop through to the infective third-stage in a natural vector, simulium ornatum. the lectins were derived from canavalia ensiformis (con a), lens culinaris (lentil), triticum vulgaris (wheat germ), arachis hypogaea (peanut), helix pomatia, phaseolus vulgaris (kidney bean) and tetragonolobus purpureus (asparagus pea). between 70 and 100 living parasites were examined for each d ... | 1988 | 3142948 |
| the role of lectins in blood group serology. | many lectins display blood group activity, and extracts from dolichos biflorus (anti-a1), ulex europaeus (anti-h), and vicia graminea (anti-n) seeds provide an alternative to human sera as a source of blood-typing reagents. however, the major application of lectins in blood group serology undoubtedly lies in the recognition and elucidation of red celll polyagglutination. in this respect, lectins from arachis hypogaea (anti-t/tk), salvia sclarea (anti-tn). salvia horminum (anti-tn + cad). dolicho ... | 1980 | 6998652 |
| endogenous as well as exogenous hormonal modulation of lectin binding sites in normal and neoplastic tissue of rat mammary gland. | free and sialic acid conjugated binding sites for the lectin from arachis hypogaea (peanut agglutinin, pna) have been histochemically demonstrated in normal rat mammary tissue and in n-nitrosomethylurea (nmu) induced mammary tumors of rats. the lectin binding sites were predominantly associated with secretory activities of the normal and neoplastic breast tissue (secretory pna binding sites). in normal breast tissue and in nmu induced mammary tumors the expression of secretory pna binding sites ... | 1988 | 3150553 |
| changes in peanut lectin binding sites on the neuroectoderm during neural tube formation in the bantam chick embryo. | cell surface carbohydrates in the neurulating ectoderm of bantam chick embryos of stage 6-11 were examined using the fluorescein isothiocyanate-labeled and ferritin-labeled peanut lectin, arachis hypogaea agglutinin (pna), which is gal beta 1----3galnac specific. weak fluorescence showing pna binding sites was seen on the apical surfaces of neural plate cells. on the surfaces of neural tube cells the fluorescence was more intense and appeared as a band. when using ferritin particles as a quantit ... | 1988 | 3177890 |
| circular dichroism studies on conformational transitions of phytohemagglutinins effected by some alcohols. | the effects of methanol, ethanol, 1-propanol and 2,2,2,-trifluoroethanol (tfe) on conformation of 10 phytohemagglutinins (lectins) were examined by applying the circular dichroism (cd) method. concanavalin a and the lectins isolated from pea (pisum sativum), lentil (lens culinaris), soybean (glycine max), peanut (arachis hypogaea), dolichos biflorus, sophora japonica, lotus tetragonolobus, ulex europaeus i, and phaseolus vulgaris (leucoagglutinin) were used. slight changes in the structure of th ... | 1982 | 7150429 |
| the surface glycoproteins of human skin fibroblasts detected after electrophoresis by the binding of peanut (arachis hypogaea) agglutinin and ricinus communis (castor-bean) agglutinin i. | a new methodology was developed to study the cell-surface glycoproteins of cultured human skin fibroblasts. this was based on the binding of a variety of biotinyl-lectins to nitrocellulose electrophoretic transfers of total fibroblast lysates after separation in sodium dodecyl sulphate/polyacrylamide gels, followed by reaction with avidin-biotinyl-peroxidase complexes and detection with 3,3'-diaminobenzidine. the technique proved to be very sensitive and a large number of glycoproteins were dete ... | 1982 | 7159403 |
| interaction of lectins from soybean and peanut with rhizobia that nodulate soybean, peanut, or both plants. | four of 14 strains of rhizobium japonicum from soybean nodulated peanut (arachis hypogaea l. cultivar jumbo virginia), and 3 of 8 rhizobium sp. strains from peanut nodulate soybean (glycine max (l.) merr. cultivar harosoy 63). cells of three peanut rhizobia bound fluorescent- and radioisotope-labeled soybean lectin. two of these strains failed to nodulate soybean, and conversely, two peanut strains that nodulated soybean did not bind to soybean lectin. both culture medium and age had pronounced ... | 1980 | 7195297 |
| simple test for detection of virus neuraminidase and antineuraminidase using lectins (lectin-neuraminidase test system). | the fast and sensitive detection of virus neuraminidase effectiveness (influenza virus a and b or mumps virus) and of antibodies against virus neuraminidase in human serum is described. lectin mainly obtained from arachis hypogaea and helix pomatia is used in the lectin-neuraminidase test (ln-test). the lectins are capable of agglutinating erythrocytes after virus incubation because virus neuraminidase reveals the t-antigen to be situated on erythrocytes. the presence of anti-neuraminidase in hu ... | 1980 | 7223125 |
| [neuraminidase-producing pneumococci in the pathogenesis of hemolytic-uremic syndrome]. | hemolytic-uremic syndrome (hus) accompanied by pneumococcal infections forms a characteristic subgroup of hus. pneumococcal neuraminidase splits off neuraminic acid from the glycoproteins present on the surface of red cells, thrombocytes and endothelial cells, and thus exposes the hidden thomsen cryptantigen (t-ag). the t-ag can then react with a complement-fixing antibody of the igm class which is present in all human plasmas after the age of 6 months. early diagnosis of t-transformation should ... | 1980 | 7280595 |
| [experiments to elucidate the role of aspergillus fungi in the hemorrhagic syndrome in poultry]. | attempts were made to assess the role played by fungi of the aspergillus genus in the occurrence of the hemorrhagic syndrome in birds. groups of test birds were given continuously a forage mixture containing 30 per cent of ground nut cake which had aflatoxin at the rate of 1.5 ppm, a forage mixture 30 per cent of which was preliminarily contaminated with spores of asp. fumigatus or asp. flavus, and subtoxic amounts of nonpurified aflatoxin. growth retardation was established along with the devel ... | 1981 | 7331224 |
| tracheobronchial epithelium of the sheep: iv. lectin histochemical characterization of secretory epithelial cells. | conventional histochemical characterization of the mucus secretory apparatus is often difficult to reconcile with the biochemical analysis of respiratory secretions. this study was designed to examine the secretory glycoconjugates in airways using lectins with biochemically defined affinities for main sugar residues of mucus. we used five biotinylated lectins--dba (dolichos biflorus) and sba (glycine max) for n-acetyl galactosamine (galnac), bsa i (bandeiraea simplicifolia) and pna (arachis hypo ... | 1988 | 3189886 |
| lectin binding sites on the surfaces of the pneumonocytes in human neonatal lung. | the surface coating of the pneumonocytes in human neonatal lung was studied by means of an electron microscope technique. slices of aldehyde-fixed lung tissue were labelled with a horseradish peroxidase conjugate of one of the following lectins: dolichos biflorus lectin, triticum vulgaris lectin, canavalia ensiformis lectin (concanavalin a), limulus polyphemus lectin, lotus tetragonolobus lectin and arachis hypogaea lectin. the tissue slices were then incubated in a diaminobenzidine-hydrogen per ... | 1986 | 3733468 |
| preneoplastic and neoplastic lesions of rat hereditary renal cell tumors express markers of proximal and distal nephron. | long-evans (eker) rats carry a mutation that predisposes them to develop spontaneous renal cell tumors of two morphologic patterns: solid chromophilic masses or cystic lesions lined by eosinophilic cells. previous studies have suggested that these tumors arise from the proximal tubules. in the present study, lectin-binding characteristics and cytokeratin expression of various stages of hereditary rat renal epithelial neoplasia were examined to localize the portion of the nephron from which tumor ... | 1995 | 7483212 |
| intermediate filament expression and lectin histochemical features of canine transmissible venereal tumour. | immunocharacterization of intermediate filament proteins and lectin-binding studies were carried out in canine transmissible venereal tumour (tvt), a unique neoplasia sharing some epidemiological features with kaposi's sarcoma in humans. neoplastic cells express vimentin, but neither cytokeratin nor desmin. regarding lectins, tvt cells express receptors for triticum vulgaris (wga), concanavalia ensiformis (con a) and ricinus communis i (rca-i). they appear to be negative for ulex europaeus-i (ue ... | 1995 | 7488385 |
| echinostoma caproni and e. trivolvis alter the binding of glycoconjugates in the intestinal mucosa of c3h mice as determined by lectin histochemistry. | mouse (c3h) mucosal glycoconjugates were examined in normal small intestines and intestines infected with echinostoma caproni or e. trivolvis using six different fluorescein-conjugated lectins: triticum vulgaris agglutinin (wga), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin i (rca-i), glycine max soybean agglutinin (sba), dolichos biflorus agglutinin (dba), and arachis hypogaea peanut agglutinin (pna). the expression of lectin-binding sites and the intensity of the binding of ... | 1993 | 7507137 |
| lectin-binding sites in human parathyroid tissue. | the aim of this study was to demonstrate several lectin-binding sites in human parathyroid tissue and to correlate these results with functional activity. the following lectins were tested for binding sites with certain carbohydrates (in parentheses): arachis hypogea (pna) (galactose), ulex europaeus i (uea) (fucose) and concanavalin a (cona) (mannose). in addition to normal parathyroids used as controls (13 cases), we examined adenomas associated with a clinical picture of primary hyperparathyr ... | 1986 | 3734421 |
| total substitution of leaf protein in the ration of laying hens. | laying birds of the harco strain (crossbred sex-linked brown leghorn and rhode island red) were fed rations in which leaf protein replaced 0, 25, 50, 75, and 100% of the soybean meal. these five rations were compared with a standard layer ration that contained fish meal and ground nut cake. the results show that 75% of the soybean meal in layer rations can be replaced by leaf protein without any significant effect in egg production and feed efficiency when compared with the performance of birds ... | 1986 | 3737531 |
| genetic and phenetic analyses of bradyrhizobium strains nodulating peanut (arachis hypogaea l.) roots. | seventeen bradyrhizobium sp. strains and one azorhizobium strain were compared on the basis of five genetic and phenetic features: (i) partial sequence analyses of the 16s rrna gene (rdna), (ii) randomly amplified dna polymorphisms (rapd) using three oligonucleotide primers, (iii) total cellular protein profiles, (iv) utilization of 21 aliphatic and 22 aromatic substrates, and (v) intrinsic resistances to seven antibiotics. partial 16s rdna analysis revealed the presence of only two rdna homolog ... | 1995 | 7538280 |