| ethylene regulation of fruit softening and cell wall disassembly in charentais melon. | cell wall disassembly in ripening fruit is highly complex, involving the dismantling of multiple polysaccharide networks by diverse families of wall-modifying proteins. while it has been reported in several species that multiple members of each such family are expressed in the same fruit tissue, it is not clear whether this reflects functional redundancy, with protein isozymes from a single enzyme class performing similar roles and contributing equally to wall degradation, or whether they have d ... | 2007 | 17308329 |
| one-step purification of histidine-tagged profilin with high purity and yield by using metal precipitation. | a simple one-step method for the purification of recombinant his-tagged profilin from the bacterial cell lysate is reported. noting the greater ease with which hexahistidine-tagged proteins can be metalprecipitated compared with unwanted protein impurities, we investigated the effect of lysis-buffer additives and optimization of other conditions to recover selectively desired proteins in a one-step metal precipitation without using biopolymers. purification of the his-tagged melon (cucumis melo) ... | 2007 | 17313372 |
| functional characterization of a melon alcohol acyl-transferase gene family involved in the biosynthesis of ester volatiles. identification of the crucial role of a threonine residue for enzyme activity*. | volatile esters, a major class of compounds contributing to the aroma of many fruit, are synthesized by alcohol acyl-transferases (aat). we demonstrate here that, in charentais melon (cucumis melo var. cantalupensis), aat are encoded by a gene family of at least four members with amino acid identity ranging from 84% (cm-aat1/cm-aat2) and 58% (cm-aat1/cm-aat3) to only 22% (cm-aat1/cm-aat4). all encoded proteins, except cm-aat2, were enzymatically active upon expression in yeast and show different ... | 2005 | 16247561 |
| induced systemic resistance and promotion of plant growth by bacillus spp. | abstract elicitation of induced systemic resistance (isr) by plant-associated bacteria was initially demonstrated using pseudomonas spp. and other gram-negative bacteria. several reviews have summarized various aspects of the large volume of literature on pseudomonas spp. as elicitors of isr. fewer published accounts of isr by bacillus spp. are available, and we review this literature for the first time. published results are summarized showing that specific strains of the species b. amyloliquef ... | 2004 | 18944464 |
| the relationship of host range, physiology, and genotype to virulence on cantaloupe in pseudomonas syringae from cantaloupe blight epidemics in france. | abstract in 1993, a bacterial blight caused important losses of cantaloupe (cucumis melo var. cantalupensis) in southwestern france and has now been reported in all cantaloupe-growing regions of france. the causal agent of this blight is pseudomonas syringae, although on a worldwide basis this bacterium has not been a major pathogen of melon for over 50 years. to identify the pathovar of the cantaloupe pathogen, we employed biochemical tests, plasmid and chromosomal profiling, and host range stu ... | 2000 | 18944544 |
| pathogenic and genetic variation in the japanese strains of fusarium oxysporum f. sp. melonis. | abstract pathogenic variation among 41 japanese strains of fusarium oxysporum f. sp. melonis was analyzed by pathogenicity tests with muskmelon, oriental melon, and oriental pickling melon cultivars. based on pathogenicity to muskmelon cvs. amus and ohi and oriental melon cv. ogon 9, 41 strains were divided into 3 groups that corresponded completely to risser's races 0, 2, and 1,2y. to further characterize pathogenic variation within the forma specialis and races, strains were assayed for pathog ... | 1998 | 18944886 |
| atp bioluminescence assay for estimation of microbial populations of fresh-cut melon. | estimation of microbial numbers in foods by conventional microbiological techniques takes days, so there is a need for faster methods that can give results in minutes. research was undertaken to investigate the use of bioluminescent atp determination and a firefly luciferase assay to estimate the initial population of aerobic mesophilic bacteria on fresh-cut melons immediately after preparation and during storage at 5 or 15 degrees c for up to 12 days. populations of aerobic mesophilic bacteria ... | 2005 | 16300083 |
| curtovirus-cucurbit interaction: acquisition host plays a role in leafhopper transmission in a host-dependent manner. | curly top disease (ctd) of vegetable crops is caused by viruses in the genus curtovirus (family geminiviridae). cucurbits are reported to be susceptible to ctd; however, the disease is rare in california despite annual outbreaks in other hosts (e.g., common bean, pepper, sugar beet, and tomato). consistent with these observations, no obvious curly top symptoms were observed in melon fields surveyed for ctd in central california in 2004 and 2005, whereas the disease was readily observed in tomato ... | 2009 | 19055441 |
| functional characterization of cmccd1, a carotenoid cleavage dioxygenase from melon. | carotenoids are nutritionally important tetraterpenoid pigments that upon oxidative cleavage give rise to apocarotenoid (norisoprene) aroma volatiles. beta-carotene is the predominant pigment in orange-fleshed melon (cucumis melo l.) varieties, reaching levels of up to 50 microg/gfw. pale green and white cultivars have much lower levels (0-10 microg/gfw). in parallel, beta-ionone, the 9,10 cleavage product of beta-carotene, is present (12-33ng/gfw) in orange-fleshed melon varieties that accumula ... | 2006 | 16563447 |
| effect of gaseous ozone and hot water on microbial and sensory quality of cantaloupe and potential transference of escherichia coli o157:h7 during cutting. | the effect of gaseous ozone and hot water, alone or in combination, on the sensory and microbial quality of cantaloupe melon was investigated. escherichia coli o157:h7 transmission from the rind to edible melon flesh during cutting practices was also investigated. four different treatments consisting of hot water (75 degrees c, 1min), gaseous ozone (10,000ppm, 30min), gaseous ozone supplied by carbon monoxide gas and the combination of hot water and gaseous ozone were evaluated. sensory quality ... | 2008 | 17993390 |
| occurrence and distribution of ten viruses infecting cucurbit plants in guilan province, iran. | during the 2006 and 2007 growing seasons, a systematic survey was conducted in open-field of melon (cucumis melo l.), cucumber (c. sativus l.), squash (cucurbita sp.), and watermelon (citrulus lanatus l.) crops in 16 major cucurbit-growing areas of guilan province in iran. symptomatic leaf samples were collected and screened by double-antibody sandwich elisa (das-elisa) or rt-pcr to detect zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), cucurbit aphid-borne yellows virus (cab ... | 2008 | 18564898 |
| comparative histochemical analyses of oxidative burst and cell wall reinforcement in compatible and incompatible melon-powdery mildew (podosphaera fusca) interactions. | the spatial-temporal expression patterns of oxidative burst and cell wall reinforcement were analyzed in leaves of resistant and susceptible melon (cucumis melo l.) cultivars in response to podosphaera fusca (fr.) braun & shishkoff, the main causal agent of powdery mildew in cucurbits. extensive development of powdery mildew mycelia and a progressive increase in haustorial count were recorded in the susceptible cultivar after 4d, while in the resistant cultivar powdery mildew failed to grow and ... | 2008 | 18585824 |
| salmonella litchfield outbreak associated with a hotel restaurant--atlantic city, new jersey, 2007. | on july 10, 2007, the pennsylvania department of health notified the new jersey department of health and senior services (njdhss) of three culture-confirmed cases of salmonella litchfield infection with matching pulsed-field gel electrophoresis (pfge) patterns. data from pulsenet, the national molecular subtyping network for foodborne disease surveillance, confirmed 11 cases (including the three from pennsylvania) of this rarely identified salmonella serotype in five states during a 5-week perio ... | 2008 | 18636066 |
| distribution and pathway for phloem-dependent movement of melon necrotic spot virus in melon plants. | the translocation of melon necrotic spot virus (mnsv) within tissues of inoculated and systemically infected cucumis melo l. 'galia' was studied by tissue-printing and in situ hybridization techniques. the results were compatible with the phloem vascular components being used to spread mnsv systemically by the same assimilate transport route that runs from source to sink organs. virus rnas were shown to move from the inoculated cotyledon toward the hypocotyl and root system via the external phlo ... | 2008 | 18705860 |
| molecular cloning, characterization and expression analysis of cmapx. | the rt pcr and race methods were used to obtain the cdna sequence of an apx gene of muskmelon after the leaves were induced with powdery mildew. the cdna length of the apx gene is 1,047 bp with a 750 bp orf encoded a 249 amino acid and the molecular weight of apx protein is 27.3 kda. the analysis showed that the cmapx genomic dna contained 10 extrons and 9 introns. the identity of the amino acid sequence deduced from the cdna with the apx family of other homologous members was about 74-97%. a fu ... | 2009 | 18756374 |
| mapping of genetic loci that regulate quantity of beta-carotene in fruit of us western shipping melon (cucumis melo l.). | melon (cucumis melo l.) is highly nutritious vegetable species and an important source of beta-carotene (vitamin a), which is an important nutrient in the human diet. a previously developed set of 81 recombinant inbred lines (ril) derived from group cantalupensis us western shipper market type germplasm was examined in two locations [wisconsin (wi) and california (ca), usa] over 2 years to identify quantitative trait loci (qtl) associated with quantity of beta-carotene (qbetac) in mature fruit. ... | 2008 | 18773190 |
| inheritance of resistance to watermelon mosaic virus in cucumis melo that impairs virus accumulation, symptom expression, and aphid transmission. | abstract the cucumis melo accession tgr-1551 was found to be resistant to watermelon mosaic virus (wmv, genus potyvirus, family potyviridae). the resistance resulted in a drastic and significant reduction of virus titer and infected plants were asymptomatic or exhibited mild disease symptoms. the same gene or closely linked genes restricted virus accumulation and ameliorated symptom expression. no effect was observed on virus accumulation in inoculated leaves, which suggested that the initial ph ... | 2005 | 18943018 |
| melon resistance to cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation. | abstract the pattern of accumulation of cucurbit yellow stunting disorder virus (cysdv; genus crinivirus, family closteroviridae) rna has been analyzed in several cucurbit accessions. in susceptible accessions of melon (cucumis melo), cucumber (cucumis sativus), marrow (cucurbita maxima), and squash (cucurbita pepo), cysdv rna accumulation peaked during the first to second week postinoculation in the first to third leaf above the inoculated one; younger leaves showed very low or undetectable lev ... | 2003 | 18943165 |
| a new strain of melon necrotic spot virus that is unable to systemically infect cucumis melo. | we report a new strain of melon necrotic spot virus (mnsv) that is unable to systemically infect cucumis melo. a spherical virus (w-isolate), about 30 nm in diameter like a carmovirus, was isolated from watermelons with necrotic symptoms. the w-isolate had little serological similarity to mnsv, and it did not cause any symptoms in six melon cultivars susceptible to mnsv; however, the host range of the w-isolate was limited exclusively to cucurbitaceous plants, and transmission by o. bornovanus w ... | 2008 | 18943404 |
| recovery from cucurbit leaf crumple virus (family geminiviridae, genus begomovirus) infection is an adaptive antiviral response associated with changes in viral small rnas. | a strong recovery response occurs in cantaloupe (cucumis melo) and watermelon (citrullus lanatus) infected with the bipartite begomovirus cucurbit leaf crumple virus (culcrv). this response is characterized by initially severe symptoms, which gradually become attenuated (almost symptomless). an inverse relationship was detected between viral dna levels and recovery, indicating that recovered tissues had reduced viral titers. recovered tissues also were resistant to reinfection with culcrv; i.e., ... | 2008 | 18943741 |
| internal transcribed spacer regions 1 and 2 and random amplified polymorphic dna analysis of didymella bryoniae and related phoma species isolated from cucurbits. | abstract didymella bryoniae (anamorph phoma cucurbitacearum) is the causal agent of gummy stem blight, although other phoma species are often isolated from cucurbit plants exhibiting symptoms of the disease. the molecular and phylogenetic relationships between d. bryoniae and these phoma species are unknown. isolates of d. bryoniae and phoma obtained from cucurbits grown at various geographical locations in the united states were subjected to random amplified polymorphic dna (rapd) analysis and ... | 2002 | 18944025 |
| alternative sanitizers to chlorine for use on fresh-cut "galia" (cucumis melo var. catalupensis) melon. | chlorine is commonly used to reduce microbial load in fresh-cut vegetables. however, the production of chlorinated organic compounds, such as trihalomethanes, which are potential carcinogens, has created the need to investigate the efficiency of nontraditional sanitizers and alternative techniques. the effects of 4 novel sanitizers were tested in fresh-cut "galia" melon: chlorine dioxide (clo(2)) at 3 mg/l, peracetic acid (paa) at 80 mg/l, hydrogen peroxide (h(2)o(2)) at 50 mg/l, and nisin at 25 ... | 2008 | 19021810 |
| host range and complete genome sequence of cucurbit chlorotic yellows virus, a new member of the genus crinivirus. | cucurbit chlorotic yellows virus (ccyv) causes chlorotic yellows on cucumber (cucumis sativus) and melon (cucumis melo) and is transmitted by bemisia tabaci biotype b and q whiteflies. to characterize the host range of ccyv, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. all tested cucumis spp. except cucumis anguria and cucumis zeyheri were systemically infected with ccyv, although infection rates varied among species. citrullus lanatus, cucurbita pepo, and ... | 2010 | 20465411 |
| protective role of mangifera indica, cucumis melo and citrullus vulgaris peel extracts in chemically induced hypothyroidism. | an investigation was made to evaluate the pharmacological importance of fruit peel extracts of mangifera indica (mi), citrullus vulgaris (cv) and cucumis melo (cm) with respect to the possible regulation of tissue lipid peroxidation (lpo), thyroid dysfunctions, lipid and glucose metabolism. pre-standardized doses (200mg/kg of mi and 100mg/kg both of cv and cm), based on the maximum inhibition in hepatic lpo, were administered to wistar albino male rats for 10 consecutive days and the changes in ... | 2009 | 19059228 |
| effect of hand wash agents on controlling the transmission of pathogenic bacteria from hands to food. | the goals of this study were to evaluate the effectiveness of two hand wash regimens in reducing transient bacteria on the skin following a single hand wash and the subsequent transfer of the bacteria to a ready-to-eat food item, freshly cut cantaloupe melon. the number of bacteria recovered from hands and the quantity transferred to the melon were significantly less following the use of an antibacterial soap compared with plain soap. the antimicrobial soap achieved > 3-log reductions versus esc ... | 2007 | 18095447 |
| edible alginate-based coating as carrier of antimicrobials to improve shelf-life and safety of fresh-cut melon. | the effect of malic acid and essential oils (eos) of cinnamon, palmarosa and lemongrass and their main active compounds as natural antimicrobial substances incorporated into an alginate-based edible coating on the shelf-life and safety of fresh-cut "piel de sapo" melon (cucumis melo l.) was investigated. melon pieces (50 g) were coated with alginate-based edible coating containing malic acid (ec) and eos or their active compounds before to be packed in air filled polypropylene trays and stored a ... | 2008 | 18164505 |
| complete nucleotide sequence of a new isolate of tomato leaf curl new delhi virus infecting cucumber, bottle gourd and muskmelon in thailand. | | 2008 | 18193155 |
| monitoring the incidence and causes of diseases potentially transmitted by food in australia: annual report of the ozfoodnet network, 2006. | in 2006, ozfoodnet sites reported 24,598 notifications of seven diseases or conditions that are commonly transmitted by food, representing an increase of 2.5% over the mean of the previous 5 years. the most frequently notified aetiological agents were campylobacter (15,492 notifications) and salmonella (8,331 notifications). salmonella notifications increased in 2006 by 5.2% when compared to historical reports. the most common salmonella serotype notified in australia during 2006 was salmonella ... | 2007 | 18268875 |
| construction of a fosmid library of cucumber (cucumis sativus) and comparative analyses of the eif4e and eif(iso)4e regions from cucumber and melon (cucumis melo). | a fosmid library of cucumber was synthesized as an unrestricted resource for researchers and used for comparative sequence analyses to assess synteny between the cucumber and melon genomes, both members of the genus cucumis and the two most economically important plants in the family cucurbitaceae. end sequencing of random fosmids produced over 680 kilobases of cucumber genomic sequence, of which 25% was similar to ribosomal dnas, 25% to satellite sequences, 20% to coding regions in other plants ... | 2008 | 18273646 |
| 3-methylthiopropionic acid ethyl ester, isolated from katsura-uri (japanese pickling melon, cucumis melo var. conomon), enhanced differentiation in human colon cancer cells. | the fully ripened fruit of katsura-uri japanese pickling melon ( cucumis melo var. conomon) has rarely been used for food because the midripened fruit is utilized for making pickles, but the fully ripened fruit is no longer valuable for pickles due to the fruit body being too soft. we have considered the utilization of the fully ripened katsura-uri fruit that may be used for nonpickling products, particularly if the fully ripened fruit demonstrated health benefits such as anticarcinogenic proper ... | 2008 | 18426216 |
| fungal microbiota from rain water and pathogenicity of fusarium species isolated from atmospheric dust and rainfall dust. | in order to determine the presence of fusarium spp. in atmospheric dust and rainfall dust, samples were collected during september 2007, and july, august, and october 2008. the results reveal the prevalence of airborne fusarium species coming from the atmosphere of the south east coast of spain. five different fusarium species were isolated from the settling dust: fusarium oxysporum, f. solani, f. equiseti, f. dimerum, and f. proliferatum. moreover, rainwater samples were obtained during signifi ... | 2011 | 20820862 |
| field experiments of anopheles gambiae attraction to local fruits/seedpods and flowering plants in mali to optimize strategies for malaria vector control in africa using attractive toxic sugar bait methods. | based on recent studies in israel demonstrating that attractive toxic sugar bait (atsb) methods can be used to decimate local anopheline and culicine mosquito populations, an important consideration is whether the same methods can be adapted and improved to attract and kill malaria vectors in africa. the atsb approach uses fruit or flower scent as an attractant, sugar solution as a feeding stimulant, and an oral toxin. the atsb solutions are either sprayed on vegetation or suspended in simple ba ... | 2010 | 20854666 |
| antioxidant, sugar, mineral, and phytonutrient concentrations across edible fruit tissues of orange-fleshed honeydew melon (cucumis melo l.). | orange-fleshed, non-netted honeydew ( cucumis melo l.) is a relatively new melon in the marketplace and has shown a lot of potential as an alternative to netted muskmelons (cantaloupes), which are often prone to surface contamination by enteric bacteria. orange-fleshed honeydew is a cross between orange-fleshed cantaloupe and non-netted, green-fleshed honeydew. this glasshouse study investigated the nutritional profile (phytonutrient and sugar contents) in different tissues of mature orange-fles ... | 2008 | 18454549 |
| reduction by gaseous ozone of salmonella and microbial flora associated with fresh-cut cantaloupe. | this research investigates the efficacy of gaseous ozone, applied under partial vacuum in a controlled reaction chamber, for the elimination of salmonella inoculated on melon rind. the performance of high dose, short duration treatment with gaseous ozone, in this pilot system, on the microbial and sensory quality of fresh-cut cantaloupes was also evaluated. gaseous ozone (10,000 ppm for 30 min under vacuum) reduced viable, recoverable salmonella from inoculated physiologically mature non-ripe an ... | 2008 | 18456110 |
| survival of sa11 rotavirus in fresh fruit juices of pineapple, papaya, and honeydew melon. | survival of rotavirus in fresh fruit juices of papaya (caraca papaya l.), honeydew melon (cucumis melo l.), and pineapple (ananas comosus [l.] merr.) was studied. clarified juices were prepared from pulps of ripe fruits and sterilized by ultrafiltration. one milliliter of juice from each fruit was inoculated with 20 microl of 1 x 10(6) pfu of sa11 rotavirus and sampled immediately (0-h exposure) and 1 and 3 h later at 28 degrees c. mean viral titers in juices of papaya (ph 5.1) and honeydew melo ... | 2008 | 18522042 |
| a multi-state outbreak of salmonella saintpaul in australia associated with cantaloupe consumption. | a multi-state outbreak of salmonella enterica serovar saintpaul infection occurred in australia during october 2006. a case-control study conducted in three affected jurisdictions, new south wales, victoria and australian capital territory, included 36 cases with the outbreak-specific strain of s. saintpaul identified by multiple locus variable-number tandem repeat analysis (mlva) in a faecal specimen and 106 controls. consumption of cantaloupe (rockmelon) was strongly associated with illness (a ... | 2009 | 18559128 |
| characterization of a synergistic interaction between two cucurbit-infecting begomoviruses: squash leaf curl virus and watermelon chlorotic stunt virus. | squash leaf curl virus (slcv) and watermelon chlorotic stunt virus (wmcsv) are cucurbit-infecting bipartite begomoviruses. both viruses are found in the eastern mediterranean basin but the effects of dual infection of both viruses on melon (cucumis melo l.) have not been described. 'arava' melon plants were inoculated in the greenhouse, using whiteflies, with either slcv, wmcsv, or both. control plants were exposed to nonviruliferous whiteflies or not exposed at all. following inoculation, plant ... | 2011 | 21219130 |
| effect of temperature on infection and survival of rotylenchulus reniformis. | from infestation of lettuce with preinfective females to egg deposition, populations of rotylenchulus reniformis from baton rouge, louisiana; lubbock and weslaco, texas; and mayaguez, puerto rico, required 41, 13, 7, and 7 days at 15, 20, 25, and 34 c, respectively. no nematode infection occurred at 10 c with any r. reniformis population, and the population from puerto rico did not reproduce at 15 c. nematode survival was not influenced by temperature, since populations from texas and louisiana ... | 1988 | 19290223 |
| extraordinary resistance to insecticides reveals exotic q biotype of bemisia tabaci in the new world. | a strain of the whitefly bemisia tabaci (gennadius) possessing unusually high levels of resistance to a wide range of insecticides was discovered in 2004 in the course of routine resistance monitoring in arizona. the multiply resistant insects, collected from poinsettia (euphorbia pulcherrima willd. ex klotzsch) plants purchased at a retail store in tucson, were subjected to biotype analysis in three laboratories. polyacrylamide gel electrophoresis of naphthyl esterases and sequencing of the mit ... | 2010 | 21309242 |
| pathogenicity and histopathology of rotylenchulus reniformis infecting cantaloup. | rotylenchulus reniformis was pathogenic to cantaloup (cucumis melo 'perlita') under greenhouse conditions. these findings confirm field symptoms of cantaloup infected with r. reniformis. histopathological studies show that the nematode penetrates the cortex perpendicular to the vascular system and comes to rest with the head against the endodermis in young roots. feeding stimulated the pericycle to either side of the endodermal feeding cell and caused cell hypertrophy with enlargement of the nuc ... | 1975 | 19308147 |
| distinct colonization patterns and cdna-aflp transcriptome profiles in compatible and incompatible interactions between melon and different races of fusarium oxysporum f. sp. melonis. | fusarium oxysporum f. sp. melonis snyd. & hans. (fom) causes fusarium wilt, the most important infectious disease of melon (cucumis melo l.). the four known races of this pathogen can be distinguished only by infection on appropriate cultivars. no molecular tools are available that can discriminate among the races, and the molecular basis of compatibility and disease progression are poorly understood. resistance to races 1 and 2 is controlled by a single dominant gene, whereas only partial polyg ... | 2011 | 21338485 |
| virus-specific read-through codon preference affects infectivity of chimeric cucumber green mottle mosaic viruses displaying a dengue virus epitope. | a cucumber green mottle mosaic virus (cgmmv) was used to present a truncated dengue virus type 2 envelope (e) protein binding region from amino acids 379 to 423 (eb4). the eb4 gene was inserted at the terminal end of the cgmmv coat protein (cp) open reading frame (orf). read-through sequences of tmv or cgmmv, caa-uag-caa-uua, or aaa-uag-caa-uua were, respectively, inserted in between the cp and the eb4 genes. the chimeric clones, prt, prg, and pcg+fsrtre, were transcribed into full-length capped ... | 2009 | 19325913 |
| generation of transgenic oriental melon resistant to zucchini yellow mosaic virus by an improved cotyledon-cutting method. | production of melon (cucumis melo l.) worldwide is often limited by the potyvirus, zucchini yellow mosaic virus (zymv). in order to engineer melon lines resistant to zymv, a construct containing the translatable coat protein (cp) sequence coupled with the 3' non-translatable region of the virus was generated and used to transform an elite cultivar of oriental melon (silver light) mediated by agrobacterium using an improved cotyledon-cutting method. removal of 1-mm portion from the proximal end o ... | 2009 | 19479262 |
| inactivation of snt2, a bah/phd-containing transcription factor, impairs pathogenicity and increases autophagosome abundance in fusarium oxysporum. | the soil-borne, asexual fungus fusarium oxysporum f.sp. melonis (fom) is a causal agent of muskmelon wilt disease. the current study focused on the most virulent race of fom-race 1,2. the tagged mutant d122, generated by agrobacterium tumefaciens-mediated transformation, caused the delayed appearance of initial wilt disease symptoms, as well as a 75% reduction in pathogenicity. d122 was impaired in the gene product homologous to the snt2-like transcription factor of schizosaccharomyces pombe. in ... | 2011 | 21535351 |
| sensitivity to azoxystrobin in didymella bryoniae isolates collected before and after field use of strobilurin fungicides. | isolates of didymella bryoniae (auersw.) rehm, causal agent of gummy stem blight on cucurbits, developed insensitivity to azoxystrobin in the eastern united states 2 years after first commercial use in 1998. baseline sensitivity of this fungus to azoxystrobin has never been reported. the objectives were to compare baseline sensitivities of d. bryoniae from south carolina and other locations to sensitivities of isolates exposed to azoxystrobin for one or more seasons, and to compare sensitivity i ... | 2009 | 19488995 |
| a consensus linkage map identifies genomic regions controlling fruit maturity and beta-carotene-associated flesh color in melon (cucumis melo l.). | the nutritional value and yield potential of us western shipping melon (usws; cucumis melo l.) could be improved through the introgression of genes for early fruit maturity (fm) and the enhancement of the quantity of beta-carotene (qbetac) in fruit mesocarp (i.e., flesh color). therefore, a set of 116 f(3) families derived from the monoecious, early fm chinese line 'q 3-2-2' (no beta-carotene, white mesocarp) and the andromonoecious, late fm usws line 'top mark' (possessing beta-carotene, orange ... | 2009 | 19551368 |
| a conserved ethylene biosynthesis enzyme leads to andromonoecy in two cucumis species. | andromonoecy is a widespread sexual system in angiosperms, characterized by plants carrying both male and bisexual flowers. monoecy is characterized by the presence of both male and female flowers on the same plant. in cucumber, these sexual forms are controlled by the identity of the alleles at the m locus. in melon, we recently showed that the transition from monoecy to andromonoecy result from a mutation in 1-aminocyclopropane-1-carboxylic acid synthase (acs) gene, cmacs-7. to isolate the and ... | 2009 | 19578542 |
| long-distance trafficking of macromolecules in the phloem. | the fact that macromolecules such as proteins and mrnas overcome the symplastic barriers between various tissue domains was first evidenced by the movement of plant viruses. we have recently demonstrated that viral infection disengages the symplastic restriction present between the sieve element-companion cell complex and neighboring cells in tobacco plants. as a result, green fluorescent protein, which was produced in mesophyll and bundle sheath cells, could traffic into the sieve tube and trav ... | 2008 | 19704648 |
| food reservoir for escherichia coli causing urinary tract infections. | closely related strains of escherichia coli have been shown to cause extraintestinal infections in unrelated persons. this study tests whether a food reservoir may exist for these e. coli. isolates from 3 sources over the same time period (2005-2007) and geographic area were compared. the sources comprised prospectively collected e. coli isolates from women with urinary tract infection (uti) (n = 353); retail meat (n = 417); and restaurant/ready-to-eat foods (n = 74). e. coli were evaluated for ... | 2010 | 20031048 |
| branched-chain and aromatic amino acid catabolism into aroma volatiles in cucumis melo l. fruit. | the unique aroma of melons (cucumis melo l., cucurbitaceae) is composed of many volatile compounds biosynthetically derived from fatty acids, carotenoids, amino acids, and terpenes. although amino acids are known precursors of aroma compounds in the plant kingdom, the initial steps in the catabolism of amino acids into aroma volatiles have received little attention. incubation of melon fruit cubes with amino acids and alpha-keto acids led to the enhanced formation of aroma compounds bearing the ... | 2010 | 20065117 |
| efficient dna extraction from nail clippings using the protease solution from cucumis melo. | owing to the increasing importance of genomic information, obtaining genomic dna easily from biological specimens has become more and more important. this article proposes an efficient method for obtaining genomic dna from nail clippings. nail clippings can be easily obtained, are thermostable and easy to transport, and have low infectivity. the drawback of their use, however, has been the difficulty of extracting genomic material from them. we have overcome this obstacle using the protease solu ... | 2010 | 20306236 |
| a melon pulp concentrate rich in superoxide dismutase reduces stress proteins along the gastrointestinal tract of pigs. | a melon (cucumis melo lc.) pulp concentrate (mpc) rich in superoxide dismutase (sod) activity was tested for its ability to decrease stress protein expressions along the gastrointestinal tract in a swine model. | 2011 | 20558040 |
| rapid detection of squash leaf curl virus by loop-mediated isothermal amplification. | a loop-mediated isothermal amplification (lamp) assay was employed to develop a simple and efficient system for the detection of squash leaf curl virus (slcv) in diseased plants of squash (cucurbita pepo) and melon (cucumis melo). completion of lamp assay required 30-60 min under isothermal conditions at 65 degrees c by employing a set of four primers targeting slcv. although the sensitivity of the lamp assay and the polymerase chain reaction (pcr) assay was comparable at high virus concentratio ... | 2010 | 20603151 |
| biological and molecular characterization of two distinct tomato strains of cucumber mosaic virus based on complete rna-3 genome and subgroup specific diagnosis. | cucumber mosaic virus (cmv) is one of the most important viral pathogen infecting several plant species in india. five isolates of cmv obtained from cucumber, muskmelon, tobacco and tomato from distinct geographical locations in india were analysed based on host-reactions and genome sequence. the majority of the isolates were very similar and only two isolates, tfr-in and tss-in showed distinct symptoms in tomato and high sequence diversity (77.8%) in coat protein (cp) gene. tfr-in was isolated ... | 2011 | 23637513 |
| cadmium accumulation by muskmelon under salt stress in contaminated organic soil. | human-induced salinization and trace element contamination are widespread and increasing rapidly, but their interactions and environmental consequences are poorly understood. phytoaccumulation, as the crucial entry pathway for biotoxic cd into the human foodstuffs, correlates positively with rhizosphere salinity. hypothesising that organic matter decreases the bioavailable cd(2+) pool and therefore restricts its phytoextraction, we assessed the effects of four salinity levels (0, 20, 40 and 60 m ... | 2009 | 19162301 |
| possible amelioration of atherogenic diet induced dyslipidemia, hypothyroidism and hyperglycemia by the peel extracts of mangifera indica, cucumis melo and citrullus vulgaris fruits in rats. | hitherto unknown efficacy of the peel extracts of mangifera indica (mi), cucumis melo (cm) and citrullus vulgaris (cv) fruits in ameliorating the diet-induced alterations in dyslipidemia, thyroid dysfunction and diabetes mellitus have been investigated in rats. in one study, out of 4 different doses (50-300 mg/kg), 200 mg/kg of mi and 100 mg/kg for other two peel extracts could inhibit lipidperoxidation (lpo) maximally in liver. in the second experiment rats were maintained on pre-standardized a ... | 2008 | 19276533 |
| the levels of male gametic mitochondrial dna are highly regulated in angiosperms with regard to mitochondrial inheritance. | the mechanisms that regulate mitochondrial inheritance are not yet clear, even though it is 100 years since the first description of non-mendelian genetics. here, we quantified the copy numbers of mitochondrial dna (mtdna) in the gametic cells of angiosperm species. we demonstrate that each egg cell from arabidopsis thaliana, antirrhinum majus, and nicotiana tabacum possesses 59.0, 42.7, and 73.0 copies of mtdna on average, respectively. these values are equivalent to those in arabidopsis mesoph ... | 2010 | 20605854 |
| functional analysis of the cucumisin propeptide as a potent inhibitor of its mature enzyme. | cucumisin is a subtilisin-like serine protease (subtilase) that is found in the juice of melon fruits (cucumis melo l.). it is synthesized as a preproprotein consisting of a signal peptide, nh(2)-terminal propeptide, and 67-kda protease domain. we investigated the role of this propeptide (88 residues) in the cucumisin precursor. complementary dnas encoding the propeptides of cucumisin, two other plant subtilases (arabidopsis ara12 and rice rsp1), and bacterial subtilisin e were expressed in esch ... | 2010 | 20639575 |
| cucumber (cucumis sativus) and melon (c. melo) have numerous wild relatives in asia and australia, and the sister species of melon is from australia. | among the fundamental questions regarding cultivated plants is their geographic origin and region of domestication. the genus cucumis, which includes cucumber (cucumis sativus) and melon (cucumis melo), has numerous wild african species, and it has therefore been assumed that melon originated in africa. for cucumber, this seemed less likely because wild cucumbers exist in india and a closely related species lives in the eastern himalayas. using dna sequences from plastid and nuclear markers for ... | 2010 | 20656934 |
| antimutagenic; differentiation-inducing; and antioxidative effects of fragrant ingredients in katsura-uri (japanese pickling melon; cucumis melo var. conomon). | six fragrant ingredients were identified in fully-ripened katsura-uri (japanese pickling melon; cucumis melo var. conomon). four of them were sulfur-containing compounds [methylthioacetic acid ethyl ester (mtae), acetic acid 2-methylthio ethyl ester (amte), 3-methylthiopropionic acid ethyl ester (mtpe), and acetic acid 3-methylthio propyl ester (amtp)]; and the others were benzyl acetate and eugenol. the newly identified mtae and amtp possessed antimutagenic activity as determined by their abili ... | 2010 | 20801232 |
| genetics of flavonoid, carotenoid, and chlorophyll pigments in melon fruit rinds. | external color has profound effects on acceptability of agricultural products by consumers. carotenoids and chlorophylls are known to be the major pigments of melon (cucumis melo l.) rinds. flavonoids (especially chalcones and anthocyanins) are also prominent in other fruits but have not been reported to occur in melons fruit. we analyzed the pigments accumulating in rinds of different melon genotypes during fruit development. we found that melon rind color is based on different combinations of ... | 2010 | 20815398 |
| [impacts of root-zone hypoxia stress on muskmelon growth, its root respiratory metabolism, and antioxidative enzyme activities]. | by using aeroponics culture system, this paper studied the impacts of root-zone hypoxia (10% o2 and 5% o2) stress on the plant growth, root respiratory metabolism, and antioxidative enzyme activities of muskmelon at its fruit development stage. root-zone hypoxia stress inhibited the plant growth of muskmelon, resulting in the decrease of plant height, root length, and fresh and dry biomass. comparing with the control (21% o2), hypoxia stress reduced the root respiration rate and malate dehydroge ... | 2010 | 20873618 |
| a novel member of the genus nepovirus isolated from cucumis melo in japan. | an unusual virus was isolated from a japanese cucumis melo cv. prince melon plant showing mild mottling of the leaves. the virus had a broad experimental host range including at least 19 plant species in five families, with most infected plants showing no symptoms on inoculated and uninoculated systemically infected leaves. the virus particles were spherical, approximately 28 nm in diameter, and the coat protein (cp) had an apparent molecular mass of about 55 kda. the virus possessed a bi-partit ... | 2011 | 20955084 |
| a k+ channel from salt-tolerant melon inhibited by na+. | • the possible roles of k(+) channels in plant adaptation to high na(+) conditions have not been extensively analyzed. here, we characterize an inward shaker k(+) channel, mirk (melon inward rectifying k(+) channel), cloned in a salt-tolerant melon (cucumis melo) cultivar, and show that this channel displays an unusual sensitivity to na(+) . • mirk expression localization was analyzed by reverse-transcription pcr (rt-pcr). mirk functional analyses were performed in yeast (growth tests) and xenop ... | 2010 | 21077888 |
| the genetic basis of resistance to downy mildew in cucumis spp.--latest developments and prospects. | downy mildew, caused by the oomycete pathogen pseudoperonospora cubensis, is one of the most destructive diseases of cucumber (cucumis sativus l.) and muskmelon (c. melo l.). although the process of pathogenesis is well understood, there are few disease control options available. the development and deployment of resistant cultivars is generally considered to be the best approach to control downy mildew. the recently completed sequencing of the cucumber genome provides a great opportunity for re ... | 2011 | 21318301 |
| watermelon chlorotic stunt virus (wmcsv): a serious disease threatening watermelon production in jordan. | the incidence of watermelon chlorotic stunt disease and the molecular characterization of the jordanian isolate of watermelon chlorotic stunt virus (wmcsv-[jo]) are described in this study. symptomatic leaf samples obtained from watermelon (citrullus lanatus thunb.), melon (cucumis melo l.), squash (cucurbita pepo), cucumber (cucumis sativus l.), and bottle gourd (lagenaria siceraria) plants were tested for wmcsv-[jo] infection by pcr. the virus could be detected in 8 melon and 87 watermelon sam ... | 2011 | 21399920 |
| infectious clones of tomato leaf curl palampur virus with a defective dna b and their pseudo-recombination with tomato leaf curl new delhi virus. | abstract: background: tomato leaf curl palampur virus (tolcpmv) is a bipartite begomovirus which has been reported from india and iran but infectious clones have not been obtained. we have previously shown the association of zucchini yellow mosaic virus (zymv), a potyvirus, with severe leaf curl disease of muskmelon in pakistan. however, the severity of symptoms in the field and yield losses led us to believe that some other agent, such as a begomovirus, could be associated with the disease. res ... | 2011 | 21496256 |
| ecology and management of whitefly-transmitted viruses of vegetable crops in florida. | a variety of fresh market vegetables, including watermelon and tomato are economically important crops in florida. whitefly-transmitted squash vein yellowing virus (sqvyv) was first identified in squash and watermelon in florida in 2005 and shown to cause a severe decline of watermelon vines as crops approach harvest. florida is most economically impacted by sqvyv, although the virus has been detected more recently in indiana and south carolina. the origin and evolutionary history of sqvyv, one ... | 2011 | 21549768 |
| light and electron microscopy observations of embryogenesis and egg development in the human liver fluke, opisthorchis viverrini (platyhelminthes, digenea). | eggs of most species digenean flukes hatch in the external environment to liberate larvae that seek and penetrate a snail intermediate host. those of the human liver flukes, opisthorchis viverrini, hatch within the gastrointestinal canal of their snail hosts. while adult parasites are primarily responsible for the pathology in cases of human opisthorchiasis, their eggs also contribute by inducing granulomata and in serving as nidi for gallstone formation. in view of the peculiar biology of o. vi ... | 2011 | 21786067 |
| olpidium bornovanus-mediated germination of ascospores of monosporascus cannonballus: a host-specific rhizosphere interaction. | monosporascus cannonballus, a host-specific root-infecting ascomycete, is the causal agent of a destructive disease of melon (cucumis melo l.) known as vine decline. ascospores germinate only in the rhizosphere of melon plants growing in field soil. however, no germination occurs in the rhizosphere of melon plants if the field soil is heated to temperatures >50-¦c prior to infestation with ascospores. this observation suggested that germination is mediated by one or more heat-sensitive members o ... | 2011 | 21675923 |
| histological study of organogenesis in cucumis melo l. after genetic transformation: why is it difficult to obtain transgenic plants? | melon (cucumis melo l.) is widely considered as a recalcitrant species for genetic transformation. in this study, we developed different regeneration and transformation protocols and we examined the regeneration process at different steps by histological studies. the highest regeneration rate (1.13-á-¦-á0.02 plants per explant) was obtained using cotyledon explants of the 'v+®drantais' genotype on murashige and skoog (ms) medium supplemented with 0.2-ámg/l 6-benzylaminopurine (bap) and 0.2-ámg/l ... | 2011 | 21706229 |
| Low genetic diversity of Squash vein yellowing virus in wild and cultivated cucurbits in the U.S. suggests a recent introduction. | Squash vein yellowing virus (SqVYV) isolates were collected from cultivated and weedy cucurbits representing major hosts and locations in the U.S. and analyzed to better understand the diversity and population structure. No differences in symptoms were observed in field-collected isolate source plants or subsequently inoculated greenhouse plants, and the complete genome of an SqVYV isolate from a wild cucurbit host (smellmelon, Cucumis melo var. dudaim) was highly similar (99.4% nucleotide ident ... | 2011 | 22142477 |
| microarray analysis shows that recessive resistance to watermelon mosaic virus in melon is associated with the induction of defense response genes. | resistance to watermelon mosaic virus (wmv) in melon (cucumis melo l.) accession tgr-1551 is characterized by a significant reduction in virus titer, and is inherited as a recessive, loss-of-susceptibility allele. we measured virus rna accumulation in tgr-1551 plants and a susceptible control ('tendral') by real-time quantitative polymerase chain reaction, and also profiled the expression of 17,443 unigenes represented on a melon microarray over a 15-day time course. the virus accumulated to hig ... | 2012 | 21970693 |
| arabidopsis suc1 loads the phloem in suc2 mutants when expressed from the suc2 promoter. | active loading of sucrose into phloem companion cells (ccs) is an essential process in apoplastic loaders, such as arabidopsis or tobacco (nicotiana sp.), and is even used by symplastic loaders such as melon (cucumis melo) under certain stress conditions. reduction of the amount or complete removal of the transporters catalysing this transport step results in severe developmental defects. here we present analyses of two arabidopsis lines, suc2-4 and suc2-5, that carry a null allele of the suc2 g ... | 2011 | 22021573 |
| biological and molecular characterization of a korean isolate of cucurbit aphid-borne yellows virus infecting cucumis species in korea. | surveys of yellowing viruses in plastic tunnels and in open field crops of melon (cucumis melo cultivar catalupo), oriental melon (c. melo cultivar oriental melon), and cucumber (c. sativus) were carried out in two melon-growing areas in 2014, korea. severe yellowing symptoms on older leaves of melon and chlorotic spots on younger leaves of melon were observed in the plastic tunnels. the symptoms were widespread and included initial chlorotic lesions followed by yellowing of whole leaves and thi ... | 2015 | 26673519 |
| expression and large-scale production of the biochemically active human tissue-plasminogen activator in hairy roots of oriental melon (cucumis melo). | human tissue-plasminogen activator (t-pa) is a thrombolytic protein that plays an active role in dissolving fibrin clots by fibrinolysis and in activating plasminogen to plasmin in blood vessels. t-pa and synthetic t-pa (st-pa) genes were expressed as enzymatically active form in hairy roots of oriental melon (cucumis melo l. cv. geumssaragi-euncheon) infected by agrobacterium rhizogenes. the insertion of the t-pa genes in genomic dna of transgenic hairy roots was verified by pcr. the presence a ... | 2011 | 22018738 |
| Quantitative analysis of mycoflora on commercial domestic fruits in Japan. | A comprehensive and quantitative analysis of the mycoflora on the surface of commercial fruit was performed. Nine kinds of fruits grown in Japan were tested. Overall fungal counts on the fruits ranged from 3.1 to 6.5 log CFU/g. The mean percentages of the total yeast counts were higher than those of molds in samples of apples, Japanese pears, and strawberries, ranging from 58.5 to 67.0%, and were lower than those of molds in samples of the other six fruits, ranging from 9.8 to 48.3%. Cladosporiu ... | 2011 | 21902918 |
| the fate of murine norovirus and hepatitis a virus during preparation of fresh produce by cutting and grating. | human noroviruses and hepatitis a virus (hav) are commonly associated with outbreaks occurring in restaurant establishments and catered events. food handlers are major contributing factors to foodborne illnesses initiated in the kitchen setting. in this study, transfer of hav and murine norovirus (mnv-1), a human norovirus surrogate, between produce (cucumbers, strawberries, tomatoes, cantaloupes, carrots, and honeydew melons) and common kitchen utensils (graters and knives) was investigated. th ... | 2012 | 23412721 |
| expression of small rna in aphis gossypii and its potential role in the resistance interaction with melon. | the regulatory role of small rnas (srnas) in various biological processes is an active area of investigation; however, there has been limited information available on the role of srnas in plant-insect interactions. this study was designed to identify srnas in cotton-melon aphid (aphis gossypii) during the vat-mediated resistance interaction with melon (cucumis melo). | 2012 | 23173035 |
| vat, an amazing gene conferring resistance to aphids and viruses they carry: from molecular structure to field effects. | we review half a century of research on cucumis melo resistance to aphis gossypii from molecular to field levels. the vat gene is unique in conferring resistance to both a. gossypii and the viruses it transmits. this double phenotype is aphid clone-dependent and has been observed in 25 melon accessions, mostly from asia. it is controlled by a cluster of genes including cc-nlr, which has been characterized in detail. copy-number polymorphisms (for the whole gene and for a domain that stands out i ... | 2016 | 27725823 |
| bacteriocins from the rhizosphere microbiome - from an agriculture perspective. | bacteria produce and excrete a versatile and dynamic suit of compounds to defend against microbial competitors and mediate local population dynamics. these include a wide range of broad-spectrum non-ribosomally synthesized antibiotics, lytic enzymes, metabolic by-products, proteinaceous exotoxins, and ribosomally produced antimicrobial peptides (bacteriocins). most bacteria produce at least one bacteriocin. bacteriocins are of interest in the food industry as natural preservatives and in the pro ... | 2015 | 26579159 |
| cell wall-degrading enzymes of didymella bryoniae in relation to fungal growth and virulence in cantaloupe fruit. | didymella bryoniae is an important pathogen of cucurbits worldwide. virulence factors of d. bryoniae were investigated in regard to fungal growth and the production of cell wall-degrading enzymes, polygalacturonase (pg), pectate lyase (pl), pectin lyase (pnl), β-galactosidase (β-gal) and cellulase (cx). virulence levels of five d. bryoniae isolates were determined by the severity of inoculated cantaloupe fruit decay. the highly virulent isolates had more mycelial growth than the moderately virul ... | 2014 | 25364138 |
| functional characterization of the arabidopsis atsuc2 sucrose/h+ symporter by tissue-specific complementation reveals an essential role in phloem loading but not in long-distance transport. | atsuc2 (at1g22710) encodes a phloem-localized sucrose (suc)/h(+) symporter necessary for efficient suc transport from source tissues to sink tissues in arabidopsis (arabidopsis thaliana). atsuc2 is highly expressed in the collection phloem of mature leaves, and its function in phloem loading is well established. atsuc2, however, is also expressed strongly in the transport phloem, where its role is more ambiguous, and it has been implicated in mediating both efflux and retrieval to and from flank ... | 2008 | 18650401 |
| multicolor fluorescence imaging as a candidate for disease detection in plant phenotyping. | the negative impact of conventional farming on environment and human health make improvements on farming management mandatory. imaging techniques are implemented in remote sensing for monitoring crop fields and plant phenotyping programs. the increasingly large size and complexity of the data obtained by these techniques, makes the implementation of powerful mathematical tools necessary in order to identify informative parameters and to apply them in precision agriculture. multicolor fluorescenc ... | 2016 | 27994607 |
| comparative analysis of type iii secreted effector genes reflects divergence of acidovorax citrulli strains into three distinct lineages. | acidovorax citrulli causes bacterial fruit blotch of cucurbits, a serious economic threat to watermelon (citrullus lanatus) and melon (cucumis melo) production worldwide. based on genetic and biochemical traits, a. citrulli strains have been divided into two distinct groups: group i strains have been mainly isolated from various non-watermelon hosts, while group ii strains have been generally isolated from and are highly virulent on watermelon. the pathogen depends on a functional type iii secre ... | 2014 | 24848275 |
| agrobacterium-mediated transformation of commercial melon (cucumis melo l., cv. amarillo oro). | cotyledon explants of muskmelon (cucumis melo l., cv. amarillo oro) seedlings were co-cultivated with disarmed agrobacterium tumefaciens strain lba4404 that contained the binary vector plasmid pbi121.1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower mosaic virus 35s/β-glucuronidase (gus) chimeric gene. after infection, the cotyledon pieces were placed in induction medium conta ... | 1994 | 24193640 |
| molecular cloning and expression of cucumisin (cuc m 1), a subtilisin-like protease of cucumis melo in escherichia coli. | oral allergy syndrome resulted from plant-derived foods is frequent among adults. allergy to melon (cucumis melo) is one of the most frequent fruit allergies in iran. three different major allergens have been found in cucumis melo that cuc m 1 (cucumisin) has been identified as the major allergen of melon. cucumisin is an alkaline serine protease that it is found as a 78kda protein in precursor form. the aim of this study was production of recombinant cuc m 1 in escherichia coli (e. coli) cells ... | 2011 | 21173569 |
| sucrose transporter plays a role in phloem loading in cmv-infected melon plants that are defined as symplastic loaders. | based on the high density of plasmodesmata interconnecting the intermediary cells and their neighboring phloem parenchyma or bundle-sheath cells, and based on the insensitivity to the sucrose transport inhibitor p-chloromercuribenzenesulfonic acid (pcmbs), cucurbits have been concluded to be symplastic loaders. in the present study, we identified and characterized the full-length sequence of sucrose transporter gene (cmsut1) from melon (cucumis melo l. cv. hale's best jumbo). in vitro experiment ... | 2011 | 21241389 |
| gibberellins producing bacillus methylotrophicus ke2 supports plant growth and enhances nutritional metabolites and food values of lettuce. | the nutritional quality of green leafy vegetables can be enhanced by application of plant beneficial micro-organisms. the present study was aimed to increase the food values of lettuce leaves by bacterial treatment. we isolated bacterial strain ke2 from kimchi food and identified as bacillus methylotrophicus by phylogenetic analysis. the beneficial effect of b. methylotrophicus ke2 on plants was confirmed by increasing the percentage of seed germination of lactuca sativa l., cucumis melo l., gly ... | 2016 | 27721133 |
| establishment of an agrobacterium-mediated inoculation system for cucumber green mottle mosaic virus. | the infectious full-length cdna clones of cucumber green mottle mosaic virus (cgmmv) isolates kw and kom, which were isolated from watermelon and oriental melon, respectively, were constructed under the control of the cauliflower mosaic virus 35s promoter. we successfully inoculated nicotiana benthamiana with the cloned cgmmv isolates kw and kom by agrobacterium-mediated infiltration. virulence and symptomatic characteristics of the cloned cgmmv isolates kw and kom were tested on several indicat ... | 2015 | 26674677 |
| antisense repression of sucrose phosphate synthase in transgenic muskmelon alters plant growth and fruit development. | to unravel the roles of sucrose phosphate synthase (sps) in muskmelon (cucumis melo l.), we reduced its activity in transgenic muskmelon plants by an antisense approach. for this purpose, an 830 bp cdna fragment of muskmelon sucrose phosphate synthase was expressed in antisense orientation behind the 35s promoter of the cauliflower mosaic virus. the phenotype of the antisense plants clearly differed from that of control plants. the transgenic plant leaves were markedly smaller, and the plant hei ... | 2010 | 20138160 |
| antisense suppression of an acid invertase gene (mai1) in muskmelon alters plant growth and fruit development. | to unravel the roles of soluble acid invertase in muskmelon (cucumis melo l.), its activity in transgenic muskmelon plants was reduced by an antisense approach. for this purpose, a 1038 bp cdna fragment of muskmelon soluble acid invertase was expressed in antisense orientation behind the 35s promoter of the cauliflower mosaic virus. the phenotype of the antisense plants clearly differed from that of control plants. the transgenic plant leaves were markedly smaller, and the stems were obviously t ... | 2008 | 18641398 |
| molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran. | fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ... | 2011 | 23961146 |
| transcriptome analysis of the melon-fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants. | fusarium oxysporum f. sp. melonis snyd. & hans race 1.2 (fom1.2) is the most virulent and yield-limiting pathogen of melon (cucumis melo l.) worldwide. current information suggest that the resistance to race 1.2 is controlled by multiple recessive genes and strongly affected by the environment. rna-seq analysis was used to identify candidate resistance genes and to dissect the early molecular processes deployed during melon-fom1.2 interaction in the resistant doubled haploid line nad and in the ... | 2017 | 28367157 |
| agroindustrial composts to reduce the use of peat and fungicides in the cultivation of muskmelon seedlings. | environmental concerns about peat extraction in wetland ecosystems have increased. therefore, there is an international effort to evaluate alternative organic substrates for the partial substitution of peat. the aim of this work was to use different composts (c1-c10) obtained from the fruit and vegetable processing industry (pepper, carrot, broccoli, orange, artichoke residues, sewage sludge (citric and pepper) and vineyard pruning wastes) to produce added-value composts as growing media with su ... | 2017 | 27197924 |
| marker-assisted selection of fusarium wilt-resistant and gynoecious melon (cucumis melo l.). | in this study, molecular markers were designed based on the sex determination genes acs7 (a) and wip1 (g) and the domain in the fusarium oxysporum-resistant gene fom-2 (f) in order to achieve selection of f. oxysporum-resistant gynoecious melon plants. markers of a and f are cleaved amplified polymorphic sequences that distinguish alleles according to restriction analysis. twenty f1 and 1863 f2 plants derived from the crosses between the gynoecious line wi998 and the fusarium wilt-resistant line ... | 2015 | 26662419 |
| phylogeny and pathogenicity of fusarium spp. isolated from greenhouse melon soil in liaoning province. | fungi of the fusarium oxysporum are widely distributed around the world in all types of soils, and they are all anamorphic species. in order to investigate the relationships and differences among fusarium spp., 25 fusarium spp. were isolated from greenhouse melon soils in liaoning province, china. with these 25 strains, three positive control fusarium strains were analyzed using universally primed pcr (up-pcr). seventy-three bands appeared after amplification using 6 primers, and 66 of these ban ... | 2014 | 25183948 |
| identification of predictor parameters to determine agro-industrial compost suppressiveness against fusarium oxysporum and phytophthora capsici diseases in muskmelon and pepper seedlings. | the lack of reliable prediction tools for evaluation of the level and specificity of compost suppressiveness limits its application. in our study, different chemical, biological and microbiological parameters were used to evaluate their potential use as a predictor parameter for the suppressive effect of composts against fusarium oxysporum f. sp. melonis (fom) and phytophthora capsici (p. capsici) in muskmelon and pepper seedlings respectively. composts were obtained from artichoke sludge, chopp ... | 2015 | 25074864 |
| development and utility of cleaved amplified polymorphic sequences (caps) and restriction fragment length polymorphisms (rflps) linked to the fom-2 fusarium wilt resistance gene in melon (cucumis melo l.). | fusarium wilt, caused by fusarium oxysporum schlecht f. sp. melonis snyder & hans, is a worldwide soil-borne disease of melon (cucumis melo l.). resistance to races 0 and 1 of fusarium wilt is conditioned by the dominant gene fom-2. to facilitate marker-assisted backcrossing with selection for fusarium wilt resistance, we developed cleaved amplified polymorphic sequences (caps) and restriction fragment length polymorphisms (rflp) markers by converting rapd markers e07 (a 1.25-kb band) and g17 (a ... | 1999 | 22665178 |
| comparative morphology of minute intestinal fluke eggs that can occur in human stools in the republic of korea. | the egg morphology of minute intestinal flukes (mif) that can occur as human infections in the republic of korea, i.e., metagonimus yokogawai, m. miyatai, m. takahashii, heterophyes nocens, heterophyopsis continua, stellantchasmus falcatus, stictodora fuscata, pygidiopsis summa, and gymnophalloides seoi, was studied in comparison with clonorchis sinensis. the adult worms were obtained from residents of endemic areas, and their intrauterine eggs were studied and measured using light microscopy; t ... | 2012 | 22949747 |