Publications

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protein enrichment of sugar beet residue with the inoculation of conidia of trichoderma album by solid state fermentation.cellulosic material was inoculated with trichoderma album by solid state fermentation and the change of protein, total nitrogen, mineral nitrogen, cellulose and total fiber contents of raw material were measured. it was found that the water holding capacity and bulk density of substrate increased as the fermentation progressed. the optimum c/n ratio for the conidia production was between 4 and 7, and the optimum ph was around 5. the protein enrichment of sugar beet residue were the best at the i ...19863816356
[effect of sugar beet saponins on the body of animals].a study was made of the effect of sugar beet saponins on motor activity of animals, energy, lipid and carbohydrate metabolism, osmotic resistance of red blood cells, antitoxic liver function. the 50- and 100-fold increase of the initial dose gave rise to an elevation of motor activity, lowering of the content of pre-beta-lipoproteins and increase of the amount of alpha-lipoproteins in blood serum, reduction of the osmotic resistance of red blood cells, dissociation of the processes of oxidation ...19854082523
development of infection with beet western yellows virus in the sugarbeet. 19724113243
swayback (enzootic ataxia) in alberta lambs.swayback (enzootic ataxia), a disease not previously described in canada, occurred in newborn lambs in alberta in 1972. of 100 lambs born in one flock, over 60 succumbed in the enzootic. the diagnosis was based on the presence of a) gross cavitations and gelatinous lesions of the cerebral white matter in 16 of 24 (66.67%) lambs examined, b) central chromatolysis and hyalinization of neurons of the red and vestibular nuclei and reticular formation and of the lateral and ventral horns of the spina ...19744274817
[lysine-supplemented rations of wheat and unextracted fresh sugar beet chips as compared with standard type swine fattening meal and wheat fish meat ration]. 19744433238
[poisoning of cows by sugar beets and corncobs]. 19744466061
an outbreak of mycotic abortion apparently due to mould-infested sugar beet pulp. 19734589483
[a case of cattle poisoning by sugar beets]. 19724662350
biotin production and utilization in a sewage treatment lagoon.biotin, in a sewage oxidation lagoon also receiving potato processing wastes, was observed to increase two logs during the summer period of waste stabilization and then to decline to near earlier concentrations. three organisms, aerobacter aerogenes, chlorella vulgaris, and thiocapsa floridana, were at least partially responsible for these fluctuations; the latter two organisms were associated with biotin utilization and the former with biotin production. since copious quantities of biotin are p ...19714939123
the use of thygesen's probang in the treatment of bovine esophageal obstruction due to sugar beets. 19725055716
ultrastructure of sugarbeet leaves infected with beet western yellows virus. 19725066415
free amino acids in sugar beet leaves and their effect upon growth of (parasitic fungus) cercospora beticola sacc. 19705457494
[relations between feeding of sugar-beet-top silage and fertility in dairy herds]. 19705466235
[compatibility of boiled sugar beet chips containing 6 per cent urea]. 19685752122
a survey of plants for leaf peroxisomes.leaves of 10 plant species, 7 with photorespiration (spinach, sunflower, tobacco, pea, wheat, bean, and swiss chard) and 3 without photorespiration (corn, sugarcane, and pigweed), were surveyed for peroxisomes. the distribution pattern for glycolate oxidase, glyoxylate reductase, catalase, and part of the malate dehydrogenase indicated that these enzymes exist together in this organelle. the peroxisomes were isolated at the interface between layers of 1.8 to 2.3 m sucrose by isopycnic nonlinear ...19695775848
[study of pseudomonas aptata brown et jamieson--agent of sugar beet bacteriosis in the ukrainian ssr]. 19655868319
[chopped sugar beet leaf as possible cause of disease in cattle]. 19655895527
studies on the antibiosis of streptomyces roseofulvus 210 against bacillus mycoides infection of sugar beet.streptomyces roseofulvus 210 isolated from the rhizosphere of sugar beet demonstrated antibiotic properties against bacillus mycoides. antibiotic produced was of macrolide character, it was able to inhibit the growth of bacillus mycoides in the soil and to reduce the infection of sugar beet seedlings in micropot and pot experiments.19846083710
toxicologic studies with lambs fed sugar beets grown on municipal sludge-amended soil: lowered relative hemoglobin in red blood cells and mutagens in blood and excreta.sugar beets grown on municipal sludge-amended soil were fed to growing lambs for 66 days. the relative hemoglobin content was significantly lower (p less than 0.05) in the lambs fed the sludge-grown sugar beets. the concentration of direct-acting mutagens was significantly higher (p less than 0.05) than controls in blood and urine of the lambs fed the sludge-grown beets. cadmium concentration was higher, but not significantly (p greater than 0.05) in the livers and kidneys of the lambs fed the s ...19846098204
interaction between bacterial metabolites and some pesticides. ii. change of phytotoxicity of the herbicide roneet by the phenolic metabolites of arthrobacter sp.the bacteria from arthrobacter genus isolated from sugar beet rhizosphere were found to produce gallic, protocatechuic, p-hydroxybenzoic, syryngic, vanillic, veratric acids, p-quinone and two unidentified phenolic compounds. the mixture of the bacterial phenolic compounds increased the phytotoxicity of roneet, inhibiting the germination of wheat. model experiments showed that the phenolic acids used with the herbicide roneet increased its phytotoxicity and p-hydroxybenzoic acid was found to be t ...19846209927
fermentation of dietary fibre components in the rat intestinal tract.1. the fermentative breakdown of dietary fibre from various sources in the intestinal tract was studied using rat balance experiments and gas-liquid chromatograhic analysis of dietary fibre monomers in feed and faces. 2. on a basal diet with 690 g maize starch/kg but no added fibre, small but detectable amounts of polymeric glucose, rhamnose, arabinose, xylose, galactose, mannose and uronic acids, i.e. sugars occurring in dietary fibre, were excreted in faeces. 3. dietary fibre in wheat bran was ...19826282300
specific inhibitors of eukaryotic dna synthesis and dna polymerase alpha, 3-deoxyaphidicolin and aphidicolin-17-monoacetate.of several phytotoxins isolated from culture filtrates of phoma betae frank ps-13, an incitant of leaf spot disease of sugar beet, three have been identified as aphidicolin, 3-deoxyaphidicolin and aphidicolin-17-monoacetate. aphidicolin is a selective inhibitor of eukaryotic dna polymerase alpha (ikegami et al. (1978) nature 275, 458-460). consequently, we studied the action mechanism of 3-deoxyaphidicolin and aphidicolin-17-monoacetate. these aphidicolin analogues markedly inhibited the in vivo ...19836402759
effect of al3+ on electron transport catalysed by photosystem i & ii of photosynthesis in cyanobacterium synechococcus spheroplasts & beet-spinach chloroplasts. 19836427098
purification and immunological properties of proton-atpase complexes from yeast and rat liver mitochondria.proton-atpase complexes from yeast and rat liver mitochondria were isolated by a simple method previously employed for the purification of the proton-atpase complex from chloroplasts. after reconstitution into liposomes, the purified complexes were active in the atp-pi exchange reaction, the rate of which was 120 and at least 200 nmol/mg of protein/min for the rat liver and yeast mitochondria atpases, respectively. upon sodium dodecyl sulfate polyacrylamide gel electrophoresis, each complex exhi ...19816455439
structure of pseudobactin 7sr1, a siderophore from a plant-deleterious pseudomonas.when grown in iron-limiting culture medium, sugar beet deleterious pseudomonas 7sr1 produced extra-cellularly the yellow-green, fluorescent siderophore pseudobactin 7sr1. pseudobactin 7sr1 had a molecular formula of c46h63n13o23 and a molecular mass of 1166 g/mol. pseudobactin 7sr1 contained a cyclic octapeptide with the amino acid sequence l-ala-gly-ser-ser-threo-beta-oh-asp-thr-ser-n delta-oh-orn. since pseudobactin 7sr1 was not affected by nonspecific enzymes, it might contain d-amino acids. ...19846466652
effect of lasalocid sodium on rumen fermentation and digestion in sheep.four adult sheep were fed 4 diets successively according to a latin-square design. they were fitted with a rumen cannula and with simple cannulae at the duodenum and ileum. the basal diet (l0) was composed of highly-pressed ensiled sugar beet pulp (56.2%), cereal (barley and corn: 27.6%), urea (1.5%) and wheat straw (14.1%). lasalocid sodium was added to obtain the following respective amounts: 21 ppm for diet l1, 43 ppm for diet l2 and 64 ppm for diet l3 (table 1). in the rumen, lasalocid signi ...19836648029
[effect of the polyether antibiotic monensin on rumen fermentation in fattening bulls during the feeding of rations rich in sugar beets].in an experiment with six fattening bulls the influence of the polyether antibiotic "monensin" (200 mg/animal and day) on the rumen physiologic parameters before and one, two, three and six hours resp. after the feeding of rations rich in dried sugar beet was investigated. the parameters of rumen fermentation are significantly influenced by the time of rumen fluid sampling and by the quota of dried sugar beet in the concentrated feed (40 and 80% resp.). the effect of "monensin" supplementation w ...19846712482
[comparative studies of the digestibility of wheat straw pellets with different proportions of concentrated feed in sheep and bulls. 2. rumen fermentation studies].4 sheep (wethers) and 4 bulls (130-140 kg live weight) received pellets of straw and concentrated feed of the charges 1-5 produced from the same wheat straw. the quota of wheat coarse meal in the pellets was, in the sequence 1 to 5:31.4; 29.7; 15.3; 11.5 and 7.8% (charge 5 contained in addition to this 7.4% dried sugar beet). the crude fibre content in the dm, in the same sequence, was as follows: 25.9; 28.1; 28.7; 31.7 and 34.4%. the total content of volatile fatty acids (vfa) in the rumen flui ...19846732499
[isobutylidene diurea as an npn source for ruminants. 1. rumen fermentation, digestibility of the nutrients and microbial protein synthesis in dairy cows].in 3 experiments 2 lactating dairy cows received a mixed ration consisting of 10 kg maize silage, 2.5 kg hay, 2.0 kg barley, 1 kg dried sugar-beet pulp, 1.5 kg maize starch, 0.5 kg sugar, 0.2 kg of a mineral-vitamin mixture and 0.08 kg non-protein nitrogen (npn) per animal and day. the ration contained 8.3 kg dry matter, 5.1 kefu cattle and 1.18 kg crude protein, 0.5 kg of which from npn, and was given in equal shares twice a day. urea (i) or isobutylidene diurea (ibdu), to which the animals wer ...19816895175
[possibilities of using nitrogen-containing waste products for cultivation of candida utilis. i. communication: the use of piacryl-waste sulphuric acid].piacryl-waste sulphuric acid and ammonia-urea wastewaters, nitrogen-containing products of chemical industry, have been used for cultivation of candida utilis. in our investigations, piacryl-waste sulphuric acid proved to be of special interest. the possibilities of using waste sulphuric acid during the cultivation of fodder yeast as well as in clarified and unclarified sugar beet molasses are discussed regarding the cell yields and the crude- and true protein content at various dilution rates i ...19806999772
[highly molassed sugarbeet pulp in ruminant's nutrition. iii. highly molassed dried sugarbeet pulp as the main component in feeding fattening bulls (author's transl)]. 19817040058
[highly molassed sugarbeet pulp in ruminant's nutrition. ii. concentrate with high rations of highly molassed dried sugarbeet pulp in the feeding of lactating cows (author's transl)]. 19817042274
[effect of volatile fatty acid concentration in the rumen on blood flow in the mesenteric vessels of sheep].the studies were performed on sheep with chronic rumen fistulae and implanted electromagnetic recorders of blood flow in the anterior mesenteric vein and the anterior mesenteric artery. the animals were fed the diet consisting of sugar beet, urea and mineral salts (diet i) or sugar beet and mineral salts (diet ii--nitrogen deficient). the diet caused an increase of blood flow in the mesenteric artery and the mesenteric vein: this increase reached the highest level after 2 hours since the applica ...19827183985
antibutyric effect of thiabendazole and monensin in the rumen of sheep fed sugar beet silage with urea. 19817303957
the effect of feeding sugar-beet silage and non-protein-n on rumen and blood metabolites in bulls. 19807370213
occurrence of fusarium species and zearalenone in dairy cattle feeds in vojvodina.the occurrence of fusarium spp. and zearalenone (f-2 toxin) in dairy cattle feeds was studied during a period of three years (in all seasons) in vojvodina. fusarium species were found to be very common in feeds. they were classified into 11 different species (f. chlamydosporum, f. equiseti, f. flocciferum, f. graminearum, f. lateritium, f. moniliforme, f. oxysporum, f. semitectum, f. solani, f. tabacinum and f. tricinctum). some of these species are known to produce mycotoxins. the highest distr ...19957491864
comparison of utilization of pectins from various sources by pure cultures of pectinolytic rumen bacteria and mixed cultures of rumen microorganisms.utilization of citrus, lucerne, apple and sugar beet pulp pectins by pure strains of rumen bacteria, prevotella ruminicola, lachnospira multiparus and butyrivibrio fibrisolvens was compared. additionally, the utilization of pectins by mixed rumen microorganisms was evaluated. the comparison was based on the depletion of galacturonic acid from medium, content of cellular protein in the cultures and the amount of end products of pectin fermentation in cell-free culture fluids. it was found that ci ...19947526615
determination of digestible energy values and fermentabilities of dietary fibre supplements: a european interlaboratory study in vivo.the performance of methods to determine energy conversion factors for dietary fibre (df) supplements and fermentability (d) values of their non-starch polysaccharides (nsp) was investigated. heats of combustion, digestible energy (de) and d values were determined on five df supplements in five european laboratories on five separate occasions. in each instance the df supplements were fed to juvenile male wistar rats at two doses, 50 and 100 g/kg basal diet, for 3 weeks with food and faeces collec ...19957547845
estimation of the fermentability of dietary fibre in vitro: a european interlaboratory study.five european laboratories tested a simple in vitro batch system for dietary fibre fermentation studies. the inoculum was composed of fresh human faeces mixed with a carbonate-phosphate buffer complex supplemented with trace elements and urea. five dietary fibre sources (cellulose, sugarbeet fibre, soyabean fibre, maize bran and pectin) were used by each laboratory on three occasions to determine ph, residual non-starch polysaccharides (nsp) and short-chain fatty acid production during fermentat ...19957547846
intake, digestion and small intestinal protein availability in sheep in relation to ammoniation of wheat straw with or without protein supplementation.the effects of ammoniation of wheat straw with or without supplementation of protein sources of either high (casein) or relatively low (potato protein) rumen degradability on intake and digestion were studied with four sheep in a 4 x 4 latin square design. rations offered were: (1) untreated wheat straw (uws), (2) ammoniated wheat straw (aws), (3) aws supplemented with 3.2 g casein/kg live weight (w)0.75 per d (awsc) and (4) aws supplemented with 3.9 g potato protein/kg w0.75 per d (awsp). straw ...19957547849
analysis and chromosomal localization of retrotransposons in sugar beet (beta vulgaris l.): lines and ty1-copia-like elements as major components of the genome.dna sequences of the reverse transcriptase gene of long terminal repeat (ltr) and non-ltr (non-viral) retrotransposons have been isolated and cloned from the genome of sugar beet (beta vulgaris). both retrotransposon types are highly amplified in sugar beet and may account for 2-5% of the genome. the bnr1 family, representing the first non-viral retrotransposon reported from a dicotyledonous species, shows homology to the mammalian l1 family of long interspersed repeated sequences (lines) and to ...19957551548
detection of beet necrotic yellow vein virus using reverse transcription and polymerase chain reaction.a diagnostic test based on reverse transcription followed by the polymerase chain reaction (rt-pcr) was developed for the detection of beet necrotic yellow vein virus (bnyvv). a specific 500-base-pair fragment was amplified from the read-through region of the coat protein gene located on rna-2. the viral origin of the amplified product was confirmed by sequencing, with the sequence obtained having 94.5% homology with published sequence data for bnyvv. the assay gave a sensitivity of 800 times th ...19957559854
cloning, sequence and expression of the gene coding for rhamnogalacturonase of aspergillus aculeatus; a novel pectinolytic enzyme.rhamnogalacturonase was purified from culture filtrate of aspergillus aculeatus after growth in medium with sugar-beet pulp as carbon source. purified protein was used to raise antibodies in mice and with the antiserum obtained a gene coding for rhamnogalacturonase (rhga) was isolated from a lambda cdna expression library. the cloned rhga gene has an open-reading frame of 1320 base pairs encoding a protein of 440 amino acids with a predicted molecular mass of 45 962 da. the protein contains a po ...19957576553
pcr and non-isotopic labeling techniques for plant virus detection.pcr technology permits the detection of viruses at levels several orders of magnitude lower than is possible by other methods. this high sensitivity facilitates detection of virus sequences during the early stages of infection of plants and in soil and vector samples. early detection of beet necrotic yellow vein virus (bnyvv) in beta vulgaris is an important part of the strategy for prevention of the spread of rhizomania, a commercially significant disease of sugar beet. a diagnostic test for bn ...19957580844
construction of a rhizosphere pseudomonad with potential to degrade polychlorinated biphenyls and detection of bph gene expression in the rhizosphere.the genetically engineered transposon tnpcb, contains genes (bph) encoding the biphenyl degradative pathway. tnpcb was stably inserted into the chromosome of two different rhizosphere pseudomonads. one genetically modified strain, pseudomonas fluorescens f113pcb, was characterized in detail and found to be unaltered in important parameters such as growth rate and production of secondary metabolites. the expression of the heterologous bph genes in f113pcb was confirmed by the ability of the genet ...19957646029
characterization and localization of new antifungal cysteine-rich proteins from beta vulgaris.two novel antifungal proteins, ax1 and ax2, were isolated from leaves of sugar beet infected with cercospora beticola. ax1 (mw = 5078 +/- 3d) and ax2 (mw = 5193 +/- 3d) were n-terminally sequenced and identified as monomeric, basic proteins consisting of 46 amino acid residues, of which eight are cysteines. both ax proteins strongly inhibit growth of c. beticola and other filamentous fungi, but have little or no effect against bacteria. based on primary sequence homology (24 to 46% identity), th ...19957655063
[food conversion in the digestive system of juvenile bulls. 1. conversion of protein and amino acids].fistulated bulls (duodenal bridge fistulas) received 10 rations containing 60% concentrate and combinations of carbohydrates from various sources to investigate the protein and amino acid flux in the digestive tract. with a constant crude protein concentration of 12-13% in the rations (adjusted by adding urea), an average of 85 +/- 8% of the crude protein ingested with the feed were found in the duodenum. the highest values were measured for rations containing wheat/dried sugar beet chips (97% a ...19947668969
[food conversion in the digestive system of juvenile bulls. 3. rumen liquor investigations].two bulls with a permanent rumen fistula and a re-entrant cannula at the duodenum were fed various concentrates and straw (dry matter ration composition: 60% concentrate, 40% straw). negative correlations were found between the ph value and total fatty acid concentration (r = -0.70). the animals were given the straw one hour before the concentrate. the ph value increased to not more than 7.3 one hour after they had received the straw. it did not drop below 6.4 after the concentrate with any of t ...19947668978
[dry matter and nutrient degradability of foodstuffs after incubation in the rumen (nylon bag technique)].the digestion of dry matter and nutrients of wheat/barley, maize, potatoes, dried sugar beet chips dried sugar beet pulp and barley straw were studied by means of the nylon bag method on two steers with fistulated rumens. compared with that of the other feedstuffs with a high starch content, the disappearance of maize was significantly lower in terms of dry matter (20%), starch (38%) and crude protein (23%). in the case of cereals, dry matter degradability correlated closely with the disappearan ...19947668979
beet fed as such or ensiled with maize and fresh potatoes in diets for finishing bulls.the use of beet for beef production after ensiling together with maize was studied in two experiments involving 112 and 114 white-blue bulls. in the first experiment maize silage (i) was compared with mixed silage of maize and fodder beet (ii) or sugar beet (iii) (ratio: 2/1, dm-basis). in the second experiment maize silage (i) was compared with mixed silage of 0.67 maize and 0.33 sugar beet (ii) or maize silage and fresh sugar beet (ratio: 2/1, dm basis) (iii) or fresh sugar beet and potatoes a ...19947733816
cloning of the aspergillus niger gene encoding alpha-l-arabinofuranosidase a.using l-arabitol as an inducer, simple induction conditions were established that resulted in high-level expression of alpha-l-arabinofuranosidase a by an aspergillus niger d-xylulose kinase mutant strain. these conditions were adapted to construct a cdna expression library from which an alpha-l-arabinofuranosidase a cdna clone was isolated using specific antiserum. the corresponding gene encoding alpha-l-arabinofuranosidase a (abfa) was isolated from a genomic library and cloned into a high cop ...19937764056
molecular cloning, expression and structure of the endo-1,5-alpha-l-arabinase gene of aspergillus niger.secretion of endo-1,5-alpha-l-arabinase a (abn a) by an aspergillus niger xylulose kinase mutant upon mycelium transfer to medium containing l-arabitol was immunochemically followed with time to monitor its induction profile. a cdna expression library was made from polya+ rna isolated from the induced mycelium. this library was immunochemically screened and one abn a specific clone emerged. the corresponding abna gene was isolated from an a. niger genomic library. upon southern blot analysis, a ...19937764386
metabolic engineering of glycine betaine synthesis: plant betaine aldehyde dehydrogenases lacking typical transit peptides are targeted to tobacco chloroplasts where they confer betaine aldehyde resistance.certain higher plants synthesize and accumulate glycine betaine, a compound with osmoprotectant properties. biosynthesis of glycine betaine proceeds via the pathway choline-->betaine aldehyde-->glycine betaine. plants such as tobacco (nicotiana tabacum l.) which do not accumulate glycine betaine lack the enzymes catalyzing both reactions. as a step towards engineering glycine betaine accumulation into a non-accumulator, spinach and sugar beet complementary-dna sequences encoding the second enzym ...19947764986
microbial production of propionic acid and vitamin b12 using molasses or sugar.with a cell concentration of 125 g dry biomass l-1 and a dilution rate of 0.1 h-1, propionibacterium acidipropionici produces 30 g propionic acid l-1 from sugar with a productivity of 3 g l-1 h-1. the yield of propionic acid is approx. 0.36-0.45 g propionic acid g-1 sucrose and is independent of the dilution rate and cell concentration. acetic acid is an unwanted by-product in the production of propionic acid. the concentration of acetic acid only increases slightly when the cell concentration i ...19947765100
cloning of genes required for hypersensitivity and pathogenicity in pseudomonas syringae pv. aptata.a genomic library of pseudomonas syringae pv. aptata strain ncppb 2664, which causes bacterial blight of sugar beet, lettuce and other plants, was constructed in the cosmid vector pcpp31. the 13.4 kb ecori fragment of the cosmid phir11, containing the hrp (hypersensitive response and pathogenicity) gene cluster of the closely related bacterium pseudomonas syringae pv. syringae strain 61, was used as a probe to identify a homologous hrp gene cluster in p. syringae pv. aptata. thirty of 2500 cosmi ...19957771767
[accumulation of heavy metals (pb, cd) in selected varieties of vegetables].vegetables have a great share in supplying of lead and cadmium with food into the human organism. for this reason an attempt was undertaken for quantitative evaluation of accumulation of those metals in three varieties of the following vegetables: carrots, parsley, garden beet, cabbage and potato grown at identical fertilization, weather conditions and pollution. cadmium and lead were complexed with diethyldithiocarbamate, extracted into organic phase, and determined using atomic absorption spec ...19947792516
degradation of feruloylated oligosaccharides from sugar-beet pulp and wheat bran by ferulic acid esterases from aspergillus niger.the activity of two forms of ferulic acid esterase (fae) from aspergillus niger on a synthetic feruloylated substrate (methyl ferulate) and on 11 different feruloylated oligosaccharides from sugar-beet pulp and wheat bran was determined. the enzymes exhibited different specificities for the various feruloylated substrates and were more active on certain substrates of cell-wall origin than on methyl ferulate. both enzymes preferred the arabinose residue to which ferulic acid is attached in the fu ...19947805053
purification and characterization of alpha-l-arabinofuranosidase from bacillus stearothermophilus t-6.bacillus stearothermophilus t-6 produced an alpha-l-arabinofuranosidase when grown in the presence of l-arabinose, sugar beet arabinan, or oat spelt xylan. at the end of a fermentation, about 40% of the activity was extracellular, and enzyme activity in the cell-free supernatant could reach 25 u/ml. the enzymatic activity in the supernatant was concentrated against polyethylene glycol 20000, and the enzyme was purified eightfold by anion-exchange and hydrophobic interaction chromatographies. the ...19957887599
expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid.when subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (badh). we report here cloning and characterization of badh cdna from barley, a monocot, and the expression pattern of a badh transcript. an open reading frame of 1515 bp encoded a protein which showed high homology to badh enzymes present in other ...19957888620
restriction fragment length polymorphism analysis of reverse transcription-pcr products reveals the existence of two major strain groups of beet necrotic yellow vein virus.beet necrotic yellow vein virus (bnyvv)-infected sugarbeets were obtained from many parts of europe and also from some sites in asia and the u.s.a. reverse transcription (rt)-pcr products of more than 1 kbp were obtained for four different regions of the viral genome which may be particularly important with respect to the pathogenic properties of the virus, i.e. for the coat protein and the 42k protein-encoding regions on rna 2 and for major parts of rnas 3 and 4. restriction fragment length pol ...19947913953
effect of ammonia treatment of wheat straw with or without supplementation of potato protein on intake, digestion and kinetics of comminution, rumen degradation and passage in steers.untreated wheat straw (uws) or ammoniated wheat straw without (aws) or with (awsp) a supplement of potato protein of a low rumen degradability was fed to three steers according to a 3 x 3 latin square design. all rations were supplemented with sugar-beet pulp and minerals. voluntary organic matter intake (omi, g/kg0.75 per d) was 67.8, 76.0 and 80.1 for whole rations (51.1, 59.7 and 59.2 for straw) for uws, aws and awsp respectively, which was significantly higher for aws and awsp than for uws. ...19947918323
actual medical and ergonomic problems in agriculture in the ukraine.a new agrarian policy in a number of countries caused the necessity to introduce additional measures aimed at preventing of agricultural workers' health. the epidemiological studies in the ukraine revealed the prevalence of cardiovascular diseases (ischemic heart disease, arterial hypertension) particularly among operators of agricultural machines and workers being in contact with pesticides. the state of health of women working in greenhouses and those in sugar beet growing is assessed. the rat ...19947921900
on the identification of group ii introns in nucleotide sequence data.four different consensus sequences (gti, group ii identifiers) have been derived from domains v of known group ii introns and are used as query input sequences for sensitive database screenings with the fasta and lfasta programs. the set of four gti sequences can identify all domains v of the 96 known group ii introns in the completely sequenced chloroplast genomes of marchantia polymorpha, epifagus virginiana, oryza sativa, nicotiana tabacum and the completely sequenced mitochondrial genomes of ...19947932698
the effects of dietary sugar-beet fibre and guar gum on lipid metabolism in wistar rats.this study investigates the mechanisms of action for the hypocholesterolaemic effects of sugar-beet fibre (sbf) and guar gum. four groups of ten male wistar rats were fed ad lib. on test diets containing either 100 g sbf or guar/kg, or control diets containing 100 g cellulose or wheat bran/kg for 28 d. food intake, weight gain and food consumption ratios were unaffected by the diets. circulating cholesterol and hepatic cholesterol concentrations were significantly lower in both sbf- and guar-fed ...19947947654
phenotypic and genotypic diversity of fluorescent pseudomonads isolated from field-grown sugar beet.a sample of 30 fluorescent pseudomonads isolated from the phyllosphere of sugar beet throughout a single growing season and shown to be closely related on the basis of fatty acid methyl ester (fame) analysis was subjected to detailed phenotypic and genotypic characterization. phenotypic traits were assessed on the basis of biochemical properties, assimilation of sole carbon sources, fame analysis, organic pyrolysate content (ms-pyrolysis), and total cellular protein profiles. with the exception ...19947952185
arabinase gene expression in aspergillus niger: indications for coordinated regulation.aspergillus niger secretes three glycosylated glycosyl hydrolases which are involved in degradation of the plant cell wall polysaccharide l-arabinan: alpha-l-arabinofuranosidases (abf) a and b, and endo-1,5-alpha-l-arabinase (abn) a. the nucleotide sequence of the previously cloned gene encoding abf a (abfa) from a. niger was determined. the coding region contains seven introns. mature abf a comprises 603 amino acids with a molecular mass of 65.4 kda as deduced from the nucleotide sequence. the ...19948000538
a hydroxyproline-containing class iv chitinase of sugar beet is glycosylated with xylose.two acidic chitinase isoforms, sp1 and sp2, have been purified to homogeneity from leaves of sugar beet (beta vulgaris) infected with cercospora beticola. sp1 and sp2 are extracellular proteins with an apparent molecular mass of 35 kda and an approximate pi of 4.2. since the only major difference was slightly diverging m(r)'s, only the sp2 chitinase was further characterized. partial amino acid sequence data for sp2 was used to generate a polymerase chain reaction (pcr) clone employed for the is ...19948018873
involvement of small intestinal motility in blood glucose response to dietary fibre in man.three dietary fibres with different physicochemical properties were studied in healthy humans for their effects on small intestinal motility and postprandial hyperglycaemia. duodeno-jejunal motor activity was evaluated electromyographically for 180 min in six subjects who had ingested a test meal composed of glucose alone or glucose with 15 g of wheat bran (wb), sugar beet (sb) or ispaghula (i) fibres. glucose and insulin concentrations were determined during the same period. each subject receiv ...19948054323
detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded rna.monoclonal antibodies to double-stranded rna (dsrna) are described for use as a universal diagnostic tool to detect infection in plants by rna viruses. crude nucleic acid extracts from plants infected with one of 25 different viruses were examined by sandwich-elisa and immunoblotting. in comparison to the corresponding controls elevated dsrna concentrations were found in 21 infected samples by elisa; virus-specific dsrna bands from 18 viruses were detected by immunoblotting. using this method th ...19948071415
effects of beet pulp and animal by-products on milk yield and in vitro fermentation by rumen microorganisms.forty-six holstein cows (30 primiparous) were assigned to one of four dietary treatments arranged as a 2 x 2 factorial experiment during wk 4 to 17 of lactation. main effects were corn versus dried sugar beet pulp and soybean meal versus animal by-product meal (mixture of meat and bone meal, feather meal, and blood meal). beet pulp replaced half of the corn at 15% of dietary dm. diet dm (mean of four treatments) contained 18% alfalfa pellets, 17.4% alfalfa hay, 17.2% corn silage, and 47.1% conce ...19948120188
effects of feeding restriction and meal pattern of a sugar beet-containing diet and control diet on nutrient digestibility, plasma lipid concentrations and postprandial triacylglycerol response in broiler chickens.broiler chickens were fed on a control diet based on maize or a diet containing sugar-beet-pulp fibre (beetfiber) at an inclusion level of 46 g/kg. diets were provided ad lib. or at a restricted level either once daily or three times daily. on days 13 and 20, chickens fed on the ad lib. control and sugar-beet-pulp-containing diets generally weighed more and had poorer feed conversion ratios than chickens given the restricted control and sugar-beet-pulp-containing diets respectively. furthermore, ...19948172868
plasmids isolated from the sugar beet phyllosphere show little or no homology to molecular probes currently available for plasmid typing.from a representative sample of bacteria, isolated from mature sugar beet leaves (beta vulgaris) grown at three separate locations in the uk, 79 (18%) were shown to contain plasmids ranging in size from 10 kb to 200 kb. a sensitive colony blot method was developed to facilitate the screening of both gram-negative and gram-positive isolates to determine the distribution of known plasmid incompatibility groups among plasmids isolated from the natural environment using the collection of inc/rep pro ...19948180694
the cloning and sequencing of coat protein gene from beet necrotic yellow vein virus.the beet necrotic yellow vein virus (bnyvv) isolate nm was isolated from sugarbeet infected with rhizomania in inner mongolia of china. the cdna of bnyvv coat protein (cp) gene was amplified from the extracted rna of bnyvv isolate nm by using the polymerase chain reaction (pcr) and cloned into pgem-7zf(+). its complete nucleotide sequence was determined by means of sanger's dideoxy-mediated chain-termination method. the result shows that cp gene of bnyvv isolate has 567 nucleotides. it shares 98 ...19938199322
[the effect of starch sources barley, maize and potatoes and their ration portions on the nutrient digestibility and energy utilization in ruminants. 4. nitrogen metabolism in the rumen].in 9 experimental periods on four adult bulls (lw 550 kg) fitted with re-entrant cannulae in the proximal duodenum isoenergetic rations were used on feeding level 1.7 with ground barley, ground maize or fresh potatoes as starch sources. the net energy parts of these concentrates in the ration amounted to 50, 25 and 10%. 50 to 80% of the ration dm consisted of dried grass and about 10% of sugar beet pulp. the dried grass supplied on an average 87, 79 and 62% of the feed crude protein. the intake ...19938215905
effects of partial replacement of ryegrass by low protein feeds on rumen fermentation and nitrogen loss by dairy cows.three rumen fermentation studies in combination with three feeding trials were carried out to investigate the effect of partial replacement of heavily fertilized perennial ryegrass by low protein feedstuffs on ph and concentrations of vfa and nh3 n in the rumen and on n excretion in milk, urine, and feces by dairy cows. feedstuffs tested were high fiber concentrate mixtures based on sugar beet pulp and soybean hulls and high starch concentrate mixtures based on corn (experiments 1 and 2), corn s ...19938227624
ryegrass versus corn starch or beet pulp fiber diet effects on digestion and intestinal amino acids in dairy cows.changes in digestion and aa supply in dairy cows were studied when fresh grass was partly replaced by concentrate mixtures based either on corn starch or sugar beet pulp fiber. treatments were tested in a latin square utilizing three lactating cows' with ruminal and intestinal cannulas. partial replacement of grass decreased cp digestibility. when high starch concentrate was fed, ndf digestibility was lower than that of the high fiber diet, mainly because of decreased ruminal digestion of ndf. f ...19938227671
the sugar beet mitochondrial genome contains an orf sharing sequence homology with the gene for the 30 kda subunit of bovine mitochondrial complex i.from a sugar beet mitochondrial dna library, we have isolated an open reading frame (orf192) showing extensive homology to the gene for the 30 kda subunit of the bovine mitochondrial complex i (nadh: ubiquinone reductase). the orf192 was found to be actively transcribed to give an rna of approximately 1.0 kb. we have designated this gene nad9. transcripts from the nad9 locus are edited by five c to u transitions, increasing similarity with the amino acid sequence of the corresponding bovine and ...19938246903
the effect of non-starch polysaccharide supplementation on circulating bile acids, hormone and metabolite levels following a fat meal in human subjects.the effects of guar gum, sugar-beet fibre (sbf) and wheat bran supplementation of a high-fat test meal were compared with an nsp-free control meal and a meal containing an equivalent amount of the ion-exchange resin cholestyramine in healthy non-obese human volunteers. their effects on gastric emptying, postprandial circulating bile acids, triacylglycerols and gastrointestinal hormone levels were studied. the in vitro binding of nsp and cholestyramine to [1-14c]glycocholic acid was measured and ...19938260476
adaptation to the digestion of non-starch polysaccharide in growing pigs fed on cereal or semi-purified basal diets.pigs (25-45 kg) were fed on either cereal or semi-purified basal diets supplemented with either high or low levels of sugar-beet pulp or wood cellulose (solka-floc). the apparent digestibility and retention of n and apparent digestibility and metabolizability of energy (ge) and the apparent digestibility of non-starch polysaccharides (nsp) and their constituent monomers were measured during weeks 2, 4 and 6 of the trial. n and ge were less well-digested, retained or metabolized from cereal basal ...19938260481
rna editing of a conserved reading frame in plant mitochondria increases its similarity to two overlapping reading frames in escherichia coli.an open reading frame (orfx) in mitochondria of the higher plants oenothera berteriana and arabidopsis thaliana is homologous to orf244 in the mitochondrial genome of marchantia polymorpha. homologous sequences are also present in carrot, potato and sugar beet. profile analysis revealed similarity to two overlapping reading frames in the escherichia coli genome. potential translation initiation at conserved ata (isoleucine) and ttg (leucine) codons is discussed. transcripts of the open reading f ...19948277947
cloning and characterization of the abfb gene coding for the major alpha-l-arabinofuranosidase (abf b) of aspergillus niger.based on amino-acid sequence data from aspergillus niger alpha-l-arabinofuranosidase b (abf b), and cyanogen bromide fragments derived thereof, deoxyoligonucleotide mixtures were designed to be employed as primers in a polymerase chain reaction (pcr) on a. niger genomic dna. this resulted in amplification of three related pcr products. the abfb gene encoding abf b was isolated from a genomic library using such an amplification product as a probe. a 5.1-kb bamhi fragment was subcloned to result i ...19938299175
evaluation and control of worker exposure to fungi in a beet sugar refinery.a study of worker exposure to airborne fungi was undertaken in a sugar beet refinery to evaluate the level of exposure and to determine if controls could be implemented that would lower these exposures. a previous study at this refinery identified one worker who reacted on challenge testing to the moldy but not the fresh sugar beet pulp, had specific immunoglobulin g to aspergillus niger, and specific immunoglobulin e to aspergillus. also, two employees were diagnosed with occupational asthma. i ...19938304278
breath 13co2 background enrichment during exercise: diet-related differences between europe and america.a traditional north american diet contains a high percentage of carbohydrates (cho) derived from c4 plants (maize, sugar cane), whereas a european diet contains primarily cho derived from c3 plants (potato, sugar beet). the natural 13c enrichment of the first type of cho is higher than that of the latter type. 13co2 production from orally ingested c4 plant-derived cho can, therefore, be used to quantify oxidation rates of orally ingested cho at rest and during exercise. recently it has been show ...19938335567
metabolic characteristics of pig colonocytes after adaptation to a high fiber diet.the capacities of viable colonic epithelial cells to metabolize glucose, glutamine and n-butyrate were studied in 30-kg pigs adapted to a high fiber (12% sugar beet fiber) or a low fiber diet. glucose and glutamine were extensively utilized but predominantly not oxidized, whereas n-butyrate oxidation accounted for 45% of n-butyrate metabolism and was not greatly affected by the presence of glucose or glutamine. with both diets, glycolysis was the major pathway accounting for glucose disappearanc ...19938381478
conversion of iq to 7-ohiq by gut microflora.the rates of conversion of 2-amino-3-methyl-3h-imidazo[4,5-f]quinoline (iq) to its reportedly mutagenic 7-keto derivative (7-ohiq) by intestinal bacteria from humans, mice, and rats were compared. iq was metabolized faster by cecal contents from rats or mice than by human fecal samples (113 and 87 mumol 7-ohiq formed/hr/g cecal contents, respectively, vs. 12.3 mumol/hr/g feces). cecal contents from germ-free rats colonized with human fecal bacteria [human flora-associated (hfa) rats] converted i ...19938383316
effects of sugar beet fiber feeding on serum lipids and binding of low-density lipoproteins to liver membranes in growing pigs.two groups of pigs were fed either a control diet or a diet containing sugar beet fiber (sbf). after 4 wk, total serum cholesterol and high-density-lipoprotein cholesterol concentrations were similar on both diets. by contrast, the fasting triacylglycerols were 21% lower (p < 0.05) and apparent feed-conversion efficiency was 47% higher (p < 0.01) on the sbf diet than on the control diet. accordingly, the effect of sbf did not appear to be mediated by an impairing effect on dietary lipid absorpti ...19938384780
influence of the ratio between wheat straw and ground barley, ground corn or dried sugar beet pulp on in sacco dry matter degradation of ryegrass and wheat straw, rumen fermentation and apparent digestibility in sheep.castrated male sheep were fed with 5 different rations varying in the straw to concentrate ratio between 100: 0, 75: 25, 50: 50, 25: 75 and 0: 100. ground barley, ground corn and dried sugar beet pulp were used as concentrate sources. chopped winter wheat straw was fed as roughage source. all rations were supplemented with a protein-mineral-vitamin-premix. in sacco dry matter degradability of artificially dried ryegrass, untreated and ammonia treated wheat straw was measured within three rumen f ...19938390235
effect of substitution of sugarbeet pulp for barley in diets for finishing rabbits on growth performance and on energy and nitrogen efficiency.one hundred forty new zealand white x california weanling rabbits were used to study the influence of increasing dietary contents of digestible fiber replacing nonstructural carbohydrates on feed efficiency and growth performance. four diets, formulated by replacing sugarbeet pulp (sbp) for barley grain, and two slaughter weights (2 or 2.5 kg) were used in a 4 x 2 factorial arrangement. inclusion of 15% sbp decreased dietary energy and cp digestibilities by 6% (p < .001), but nonsignificant effe ...19938394306
effects of increasing levels of sugar-beet pulp in broiler chicken diets on nutrient digestion and serum lipids.broiler chickens were fed on a control diet based on maize or on diets including conventionally-dried (standard pulp) or vacuum-dried (fipec pulp) sugar-beet pulp at inclusion levels of 23, 46 and 92 g/kg. diets were fed ad lib. except for half of the chickens fed on the control diet which were fed twice daily at a restricted level. although not statistically significant, chickens given the 23 g sugar-beet pulp/kg diets generally consumed more feed, had increased body weights and converted feed ...19938399094
an acidic class iii chitinase in sugar beet: induction by cercospora beticola, characterization, and expression in transgenic tobacco plants.an acidic chitinase (se) was found to accumulate in leaves of sugar beet (beta vulgaris) during infection with cercospora beticola. two isoforms, se1 and se2, with mw of 29 kda and pi of approximately 3.0 were purified to homogeneity. se2 is an endochitinase that also exhibits exochitinase activity, i.e., it is capable of hydrolyzing chito-oligosaccharides, including chitobiose, into n-acetyl-glucosamine. partial amino acid sequence data for se2 were used to obtain a cdna clone by polymerase cha ...19938400378
[toxic action of resin - regulator of sugar beet growth]. 19938406088
fermentation of raw poultry byproducts for animal nutrition.in this study, the fermentation of raw, inedible poultry byproducts mixed with sugarbeet pulp and dextrose and inoculated with lactobacillus plantarum and(or) enteroccocus faecium resulted in a drop of ph in the byproducts to approximately 4.0 to 4.5 within 48 h. to keep the fermented product stable for a period of 21 d, the addition of > or = 3% (wt/wt) of a fermentable carbohydrate was necessary. with a high inoculation level of approximately 10(8) to 10(9) l. plantarum per gram, or with acidi ...19938407654
effect of a viable yeast culture on digestibility and rumen fermentation in sheep fed different types of diets.five mature wethers fitted with rumen fistulas were fed grass hay and a sugarbeet-pulp-based concentrate or maize silage and a cereal-based concentrate (50/50 digestible organic matter basis), or without with 5 g yeast supplement (saccharomyces cerevisiae, biosaf) per day in a latin square design. diets were given for a 28-d adaptation period, followed by a 10-d collection period to determine digestibility and nitrogen retention data. afterwards, rumen samples were taken on 3 consecutive days an ...19938447942
effects of organic fertilizers on aldicarb soil biodegradation in sugar beet crops.in the present work, the influences of several organic fertilizer treatment regimens were compared as to their slowing down effect on aldicarb soil metabolism in a sugar beet crop. the organic fertilizers treatment schemes had been repeatedly applied in the past 30 years, according to a 3-year rotation cycle. the following organic fertilizers treatment regimens--which are the main ones used in the agronomy practice--were compared: treatment 1: no organic fertilizer at all; treatment 2: 40 tons c ...19938466293
assessment of fermentation in growing pigs given unmolassed sugar-beet pulp: a stoichiometric approach.in four experiments growing pigs were given a cereal-based diet alone or supplemented with unmolassed sugar-beet pulp (sbp), used as a model substrate for fermentation. the rates of production of methane and gaseous hydrogen were measured and, together with the molar proportions of volatile fatty acids (vfa) in the digesta, used in stoichiometric calculations of fermentation. the resulting estimates were only one-sixth of the observed extent of digestion of sbp. bacteriostatic levels of antibiot ...19938490004
production of feruloyl/rho-coumaroyl esterase activity by penicillium expansum, penicillium brevicompactum and aspergillus niger.extracellular esterase production by penicillium expansum, penicillium brevicompactum and aspergillus niger was determined in both liquid and solid-state culture. methyl ferulate was used as the main carbon source in liquid culture whereas wheat bran and sugar beet pulp were used in solid-state culture. extracted enzyme for each fungus showed activity in the presence of onp butyrate, methyl ferulate, methyl coumarate and two 'natural' feruloylated carbohydrate esters. higher enzyme recoveries we ...19958557619
site directed chromosomal marking of a fluorescent pseudomonad isolated from the phytosphere of sugar beet; stability and potential for marker gene transfer.a plasmid-free, non-pathogenic, ribosomal rna group 1 fluorescent pseudomonad, pseudomonas fluorescens sbw25, was selected from the microflora of sugar beet (beta vulgaris) and modified to contain constitutively expressed marker genes. by site directed homologous recombination a kx cassette [kanamycin resistance (kanr) and catechol 2,3 dioxygenase (xyle)] and a zy cassette [lactose utilization (laczy, beta-galactosidase, lactose permease)] were introduced at least 1 mbp apart on the 6.6 mbp bact ...19958564013
sequence comparison and classification of beet luteovirus isolates.three distinct sequence groups were found among partial nucleotide sequences of 38 isolates of beet western yellows virus (bwyv) and beet mild yellowing virus (bmyv) from europe, iran and the usa. the first group contains both sugar beet and oilseed rape specific isolates, and the differentiating characteristic linked to this host range specificity are 2 single base pair changes in a 1,200 nucleotide region of the genome. it is proposed that the european bwyv strains that can be transferred at l ...19958572940
construction of a modified mini-tn5 laczy non-antibiotic marker cassette: ecological evaluation of a laczy marked pseudomonas strain in the sugarbeet rhizosphere.in order to monitor the fate of genetically manipulated fluorescent pseudomonads following release into the environment, a laczy transposable cassette, lacking antibiotic resistance genes, was constructed using a put suicide plasmid delivery system. the resulting plasmid, putlaczy, can be easily used to generate laczy marked pseudomonads without having to use antibiotic resistance determinants. the laczy transposon generates random, stable transcriptional/translational fusions on integration int ...19968595865
oxidized ethyl linoleate induces mucosal hypertrophy of the large intestine and affects cecal fermentation of dietary fiber in rats.oxidized ethyl linoleate (oel) was prepared by aeration at low temperature. peroxide value (pov, meq/kg lipid) of oel was 1400; the major oxidized compounds were 9-hydroperoxy-cis, trans- and 13-hydroperoxy-trans, cis-octadecadienoate. rats fed fiber-free or sugar-beet fiber (sbf, 100g/kg diet) diets were divided into three groups for each diet, and administered oel (high oel group), oel diluted with ethyl linoleate (low oel group, pov 700) and nonoxidized ethyl linoleate (el group) through gast ...19968613881
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