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carry-over effect of host nutritional quality on performance of spruce budworm progeny.the effect of host nutritional quality on spruce budworm (choristoneura fumiferana (clemens)) parental and offspring performance was studied using field and laboratory rearing experiments, and foliar chemical analyses. foliage of balsam fir (abies balsamea (l.) mill.), white spruce (picea glauca (moench) voss) and black spruce (p. mariana (mill.) bsp) was used to rear the parental generation in the field, whereas an artificial diet was used to rear the progeny under laboratory conditions. import ...201222077965
reproductive potential of balsam fir (abies balsamea), white spruce (picea glauca), and black spruce (p. mariana) at the ecotone between mixedwood and coniferous forests in the boreal zone of western quebec.the reproductive potentials of balsam fir and white spruce (co-dominants in mixedwood forests) and black spruce (dominant in coniferous forests) were studied to explain the location of the ecotone between the two forest types in the boreal zone of quebec. four sites were selected along a latitudinal gradient crossing the ecotone. cone crop, number of seeds per cone, percentage filled seeds, and percentage germination were measured for each species. balsam fir and white spruce cone crops were sig ...200721636443
sapling leaf trait responses to light, tree height and soil nutrients for three conifer species of contrasting shade tolerance.we developed models to describe the responses of four commonly examined leaf traits (mass per area, weight, area and nitrogen (n) concentration) to gradients of light, soil nutrients and tree height in three conifer species of contrasting shade tolerance. our observational dataset from the sub-boreal spruce forests of british columbia included subalpine fir (abies lasioscarpa [hook.] nutt; high shade tolerance), interior spruce (picea glauca × picea engelmannii [moench] voss; intermediate shade ...201425422385
indirect and mitigated effects of pulsed resources on the population dynamics of a northern rodent.pulsed resources have significant effects on population and community dynamics in terrestrial ecosystems. mast seeding is an important resource pulse in deciduous forests; these boom and bust cycles of seed production generate strong lagged population responses by post-dispersal seed predators such as rodents, which then cascade through multiple trophic levels and regulate population dynamics of their predators and prey. however, similar interactions in another major pulsed system, coniferous fo ...201323461538
changes in hormone flux and signaling in white spruce (picea glauca) seeds during the transition from dormancy to germination in response to temperature cues.seeds use environmental cues such as temperature to coordinate the timing of their germination, allowing plants to synchronize their life history with the seasons. winter chilling is of central importance to alleviate seed dormancy, but very little is known of how chilling responses are regulated in conifer seeds. white spruce (picea glauca) is an important conifer species of boreal forests in the north american taiga. the recent sequencing and assembly of the white spruce genome allows for comp ...201526680643
expression of panac01, a picea abies cup-shaped cotyledon orthologue, is regulated by polar auxin transport and associated with differentiation of the shoot apical meristem and formation of separated cotyledons.during embryo development in most gymnosperms, the establishment of the shoot apical meristem (sam) occurs concomitantly with the formation of a crown of cotyledons surrounding the sam. it has previously been shown that the differentiation of cotyledons in somatic embryos of picea abies is dependent on polar auxin transport (pat). in the angiosperm model plant, arabidopsis thaliana, the establishment of cotyledonary boundaries and the embryonal sam is dependent on pat and the expression of the c ...201222778149
the endo-1,4-β-glucanase korrigan exhibits functional conservation between gymnosperms and angiosperms and is required for proper cell wall formation in gymnosperms.the evolution of compositional polymers and their complex arrangement and deposition in the cell walls of terrestrial plants included the acquisition of key protein functions. a membrane-bound endoglucanase, termed korrigan (kor), has been shown to be required for proper cellulose synthesis. to date, no extensive characterization of the gymnosperm kor has been undertaken. characterization of the white spruce (picea glauca) gene encoding kor (pgkor) shows conserved protein features such as polari ...201222150158
a white spruce gene catalog for conifer genome analyses.several angiosperm plant genomes, including arabidopsis (arabidopsis thaliana), rice (oryza sativa), poplar (populus trichocarpa), and grapevine (vitis vinifera), have been sequenced, but the lack of reference genomes in gymnosperm phyla reduces our understanding of plant evolution and restricts the potential impacts of genomics research. a gene catalog was developed for the conifer tree picea glauca (white spruce) through large-scale expressed sequence tag sequencing and full-length cdna sequen ...201121730200
constancy of nitrogen turnover kinetics in the plant cell: insights into the integration of subcellular n fluxes.compartmental analysis with 13n was used to determine cytosolic nitrate (no3-) pools, and their turnover rates, in roots of intact barley (hordeum vulgare l. cv klondike) seedlings. influx, efflux, flux to the vacuole and assimilation, and flux to the xylem, varied as much as 300-fold over a wide range of external no3- conditions. by contrast, the kinetic constant kc describing cytosolic no3- turnover varied by less than 4% from a mean value of 0.0407 min(-1). accordingly, cytosolic no3- pools v ...200111469581
insect herbivory (choristoneura fumiferana, tortricidea) underlies tree population structure (picea glauca, pinaceae).variation in insect herbivory can lead to population structure in plant hosts as indicated by defence traits. in annual herbaceous, defence traits may vary between geographic areas but evidence of such patterns is lacking for long-lived species. this may result from the variety of selection pressures from herbivores, long distance gene flow, genome properties, and lack of research. we investigated the antagonistic interaction between white spruce (picea glauca) and spruce budworm (sbw, choriston ...201728205578
spruce budworm feeding and oviposition are stimulated by monoterpenes in white spruce epicuticular waxes.monoterpenes, source of the distinctive odor of conifers, are generally considered plant defensive compounds. however, they are also known to act as long-range insect attractants, as they are volatile and permeate forest airspaces. moreover, they are lipid soluble and can be absorbed into plant epicuticular waxes. we test their role in short-range host plant choice by both adult females and larvae of a folivorous forest pest (choristoneura fumiferana). we conducted laboratory assays testing the ...201726463122
do offspring of insects feeding on defoliation-resistant trees have better biological performance when exposed to nutritionally-imbalanced food?white spruce (picea glauca (moench) voss) trees that are resistant or susceptible to spruce budworm (choristoneura fumiferana (clem.)) attack were identified in a southern quebec plantation. due to high mortality-induced selective pressures imposed by resistant trees on spruce budworm larvae, insects that survive on resistant trees exhibited greater biological performance than those on susceptible trees. we tested the hypothesis that this better biological performance is maintained across genera ...201526463069
expression of the β-glucosidase gene pgβglu-1 underpins natural resistance of white spruce against spruce budworm.periodic outbreaks of spruce budworm (sbw) affect large areas of ecologically and economically important conifer forests in north america, causing tree mortality and reduced forest productivity. host resistance against sbw has been linked to growth phenology and the chemical composition of foliage, but the underlying molecular mechanisms and population variation are largely unknown. using a genomics approach, we discovered a β-glucosidase gene, pgβglu-1, whose expression levels and function unde ...201525302566
characterization of polyketide metabolites from foliar endophytes of picea glauca.a collection of 250 foliar endophytes of picea glauca (white spruce) yielded several isolates that produced metabolites toxic to choristoneura fumiferana (spruce budworm). three of these strains were selected for further study based on their ability to be cultured and produce secondary metabolites under laboratory conditions. the culture filtrate of each was extracted and analyzed by lc-ms and lc-nmr, and the major metabolites were isolated and characterized. structures were elucidated by spectr ...200818636777
effect of a rugulosin-producing endophyte in picea glauca on choristoneura fumiferana.disease-free second instar choristoneura fumiferana (eastern spruce budworm) were placed on trees infected with a rugulosin-producing needle endophyte in two experiments. they were allowed to grow to sixth instar when survivors were collected. first, by using 3-yr old trees, a comparison was made of budworm growth on infected and uninfected trees. a second experiment used 4-yr old trees to study the effect of rugulosin content in the needle on growth. this permitted an examination of a dose resp ...200818266038
effects of kaolin-based particle film on spruce budworm (choristoneura fumiferana (lepidoptera: tortricidae)) oviposition in the laboratory.a laboratory study conducted in 2003 determined the influence of a kaolin-based particle film (surround wp crop protectant) on spruce budworm (choristoneura fumiferana (clem)) oviposition. three concentrations (15, 30 and 60 g litre(-1) spray carrier) were applied to white spruce (picea glauca (moench) voss). when presented with different paired choices of oviposition surfaces, spruce budworms laid very few egg masses overall and showed no significant (p < 0.05) preference between kaolin-coated ...200516217725
tree-ring isotopes reveal drought sensitivity in trees killed by spruce beetle outbreaks in south-central alaska.increasing temperatures have resulted in reduced growth and increased tree mortality across large areas of western north american forests. we use tree-ring isotope chronologies (δ(13) c and δ(18) o) from live and dead trees from four locations in south-central alaska, usa, to test whether white spruce trees killed by recent spruce beetle (dendroctonus rufipennis kirby) outbreaks showed evidence of drought stress prior to death. trees that were killed were more sensitive to spring/summer temperat ...201627755740
contrasting patterns of diterpene acid induction by red pine and white spruce to simulated bark beetle attack, and interspecific differences in sensitivity among fungal associates.conifers possess a suite of physiochemical defenses that protect their subcortical tissues from bark beetle - fungal complexes. these defenses include rapid induction of terpenoids and phenolics at the site of attack. studies of the distribution, induction, and bioactivity of conifer terpenoids have focused heavily on monoterpenes. we assessed induction of diterpene acids in white spruce (picea glauca) and red pine (pinus resinosa) to fungal associates of two bark beetles, and the responses of f ...201526003180
an evaluation of british columbian beetle-killed hybrid spruce for bioethanol production.the development of bioconversion technologies for production of fuels, chemicals, and power from renewable resources is currently a high priority for developed nations such as the united states, canada, and the european union as a way to improve national energy security and reduce greenhouse gas emissions. the widespread implementation of such technologies will require a sustainable supply of biomass from forestry and agriculture. forests are a major source of feedstocks for biofuels production ...200718478394
fertility-dependent effects of ectomycorrhizal fungal communities on white spruce seedling nutrition.ectomycorrhizal fungi (ecmf) typically colonize nursery seedlings, but nutritional and growth effects of these communities are only partly understood. to examine these effects, picea glauca seedlings collected from a tree nursery naturally colonized by three dominant ecmf were divided between fertilized and unfertilized treatments. after one growing season seedlings were harvested, ectomycorrhizas identified using dna sequencing, and seedlings analyzed for leaf nutrient concentration and content ...201525904341
effects of plant leachates from four boreal understorey species on soil n mineralization, and white spruce (picea glauca) germination and seedling growth.natural regeneration of white spruce (picea glauca) after disturbance has been reported to be very poor. here a study was made to determine whether c compounds released from understorey species growing together with white spruce could be involved in this regeneration failure, either by (1) changing soil nutrient dynamics, (2) inhibiting germination, and/or (3) delaying seedling growth.200515802310
inheritance of mitochondrial dna in the conifer larix.restriction fragment length polymorphisms between larix leptolepis and larix decidua were identified in heterologous hybridization experiments, using wheat mitochondrial dna probes specific for atp9, coxi, nad3/rps12, and orf25. analysis of eight individuals of each reciprocal hybrid of these two species revealed that mitochondrial dna was maternally inherited. furthermore, sequences homologous to wheat orf25 were also identified in larix gmelini, larix siberica, larix olgensis, and larix larici ...199324193487
seasonal effect on tree species classification in an urban environment using hyperspectral data, lidar, and an object- oriented approach.the objective of the current study was to analyze the seasonal effect on differentiating tree species in an urban environment using multi-temporal hyperspectral data, light detection and ranging (lidar) data, and a tree species database collected from the field. two airborne imaging spectrometer for applications (aisa) hyperspectral images were collected, covering the summer and fall seasons. in order to make both datasets spatially and spectrally compatible, several preprocessing steps, includi ...200827879863
hormonally regulated overexpression of arabidopsis wus and conifer lec1 (chap3a) in transgenic white spruce: implications for somatic embryo development and somatic seedling growth.adult conifers are still recalcitrant in clonal propagation despite significant advances in forest tree biotechnology. plant regeneration through somatic embryogenesis from explants older than mature zygotic embryos is either difficult or impossible to achieve. to investigate if ectopic expression of transcription factors involved in the induction of the embryogenic process would induce somatic embryogenesis in picea glauca (white spruce) somatic plants, we used the leafy-cotyledon1 homolog clon ...201020424847
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