| isolation and characterization of carnobacterium, lactococcus, and enterococcus spp. from cooked, modified atmosphere packaged, refrigerated, poultry meat. | the microbiota of commercially produced, cooked and modified atmosphere packaged poultry meat was followed during storage at 3.5 degrees c for up to 7 weeks. the dominant microbiota consisted of lactococcus raffinolactis (117 isolates), carnobacterium divergens (61 isolates), carnobacterium piscicola (11 isolates), lactococcus garvieae (four isolates), lactococcus lactis (one isolate) and enterococcus faecalis (three isolates). all isolates were screened for production of bacteriocins. only c. p ... | 2000 | 11139026 |
| characterization of lactic acid bacteria in the larval midgut of the keratinophagous lepidopteran, hofmannophila pseudospretella. | in two of eight hofmannophila pseudospretella specimens studied by microscopy, the larval midgut contained an unidentified micro-organism. although not seen microscopically in midgut sections, bacteria were isolated from dissected midgut. microscopy, carbohydrate utilization and ribosomal sequence data all separated the isolates into the same three classes. these were identified as lactococcus lactis, carnobacterium piscicola and, tentatively, bacillus subtilis, the first two being facultative a ... | 2001 | 11169039 |
| identification and characterization of carnobacteria associated with the gills of atlantic salmon (salmo salar l.). | using the surface plate technique, the population level of aerobic bacteria, occurring in the gills of atlantic salmon (salmo salar l.), was determined to be approximately 3 x 10(4) g(-1). of 100 isolates investigated, 58 were gram-negative. out of the 42 gram-positive isolates, 26 belonged to the carnobacteria, of which ten were further identified on the basis of 16s rdna sequence analysis and aflp fingerprinting. all were identified as carnobacterium piscicola-like. these carnobacteria strains ... | 2000 | 11249022 |
| cresol red thallium acetate sucrose inulin (ctsi) agar for the selective recovery of carnobacterium spp. | carnobacterium spp. are commonly isolated from a variety of foods, especially from meats stored under anaerobic atmospheres at refrigeration temperatures, but the role of these organisms in the spoilage of meat and meat products is yet to be determined. cresol red thallium acetate sucrose (ctas) agar was developed as a selective medium for enumeration of carnobacteria, however problems such as poor recovery of carnobacterium spp. and interference by other microorganisms have precluded its genera ... | 2001 | 11252499 |
| description of a simple detection assay for in situ production of bacteriocin on meat. | using a modification of the agar diffusion assay, in situ bacteriocin production on meat was analyzed using cooked meat medium (cmm) and sterile pork tissue (lean and fat) with carnobacterium piscicola lv17 as the producer and carnobacterium divergens lv13 as the indicator strains. contrary to what is observed in apt broth, bacteriocin production by c. piscicola lv17 occurred with growth at low inoculum levels (< or =10(4) cfu/cm2 or g of meat) on disks (10 cm2) of pork fat tissue (ph 6.58) and ... | 2001 | 11271779 |
| alkalibacterium olivoapovliticus gen. nov., sp. nov., a new obligately alkaliphilic bacterium isolated from edible-olive wash-waters. | a novel gram-positive, obligately alkaliphilic, non-sporulating, rod-shaped, flagellated bacterium is described. three different strains of the bacterium were isolated from the wash-waters of edible-olive production. the strains are motile, psychrotolerant, halotolerant, facultatively anaerobic bacteria with a ph optimum of 9.0-9.4 for two strains and 9.8-10.2 for the third. they are catalase- and oxidase-negative. a range of hexoses and some disaccharides composed of hexoses, but not pentoses a ... | 2001 | 11414268 |
| characterisation of volatile compounds produced by bacteria isolated from the spoilage flora of cold-smoked salmon. | this study investigated the volatile compounds produced by bacteria belonging to nine different bacterial groups: lactobacillus sake, l. farciminis, l. alimentarius, carnobacterium piscicola, aeromonas sp., shewanella putrefaciens, brochothrix thermosphacta, photobacterium phosphoreum and enterobacteriaceae isolated from cold-smoked salmon. each bacterial group was represented by several strains. in addition, combinations of the groups were examined as well. sterile blocks of cold-smoked salmon ... | 2001 | 11428576 |
| identification and characterization of carnobacteria isolated from fish intestine. | eleven bacterial strains were isolated from the gastrointestinal tract of four fish species, atlantic salmon (salmo salar l.), arctic charr (salvelinus alpinus l.), atlantic cod (gadus morhua l.) and wolffish (anarhichas lupus l.). all the strains were gram-positive rods, non-sporing, catalase and oxidase-negative, able to grow at ph 9.0 but not on acetate containing media (ph < or = 5.4), and were fermentative. they had a high content of oleic acid (18:1 n-9) in cellular lipid, and were found t ... | 2001 | 11518321 |
| factors affecting production of an antilisterial bacteriocin by carnobacterium piscicola strain a9b in laboratory media and model fish systems. | to investigate factors influencing bacteriocin production and bacteriocin stability of the bioprotective culture carnobacterium piscicola strain a9b. | 2001 | 11556917 |
| a two-component signal-transduction cascade in carnobacterium piscicola lv17b: two signaling peptides and one sensor-transmitter. | in the lactic acid bacterium carnobacterium piscicola lv17b a peptide-pheromone dependent quorum-sensing mode is involved in the regulation of bacteriocin production. bacteriocin cb2 was identified as an environmental signal that induces bacteriocin production. here, we demonstrate that a second 24 amino acid peptide (cs) also induces bacteriocin production. transcription activation of several carnobacteriocin operons is triggered by cb2 or cs via a two-component signal transduction system compo ... | 2001 | 11587787 |
| characterization of two new glycosyl hydrolases from the lactic acid bacterium carnobacterium piscicola strain ba. | three genes with homology to glycosyl hydrolases were detected on a dna fragment cloned from a psychrophilic lactic acid bacterium isolate, carnobacterium piscicola strain ba. a 2.2-kb region corresponding to an alpha-galactosidase gene, agaa, was followed by two genes in the same orientation, bgab, encoding a 2-kb beta-galactosidase, and bgac, encoding a structurally distinct 1.76-kb beta-galactosidase. this gene arrangement had not been observed in other lactic acid bacteria, including lactoco ... | 2001 | 11679331 |
| characterization, production, and purification of carnocin h, a bacteriocin produced by carnobacterium 377. | carnocin h, a bacteriocin produced by a carnobacterium sp., inhibited lactic acid bacteria, clostridia, enterococci, and some staphylococcus aureus strains. some strains of listeria and pediococcus were also sensitive to carnocin h. the bacteriocin was produced during the late stationary growth phase. carnocin h was purified by cation exchange chromatography and reverse phase chromatography. amino acid sequence and composition indicate that carnocin h is a novel bacteriocin belonging to the clas ... | 2001 | 11683354 |
| evaluation of the ability of lysozyme and nisin to control meat spoilage bacteria. | the antimicrobials lysozyme, nisin, and mixtures of the two were studied to ascertain their abilities to control the growth of the meat-borne spoilage bacteria, brochothrix thermosphacta b2 and carnobacterium sp. 845. the goal was to optimize an antimicrobial for potential use in preservation of fresh meats. their efficacies were evaluated in apt broth, in a meat juice extract and on cores of lean and fat pork tissue. both lysozyme and nisin alone as well as mixtures of the two effectively inhib ... | 2001 | 11759749 |
| isobaculum melis gen. nov., sp. nov., a carnobacterium-like organism isolated from the intestine of a badger. | phenotypic and phylogenetic studies were performed on a hitherto undescribed facultatively anaerobic, catalase-negative, gram-positive rod-shaped organism, strain m577-94t, isolated from the small intestine of a dead badger. it resembled carnobacteria in terms of its long-chain cellular fatty acid composition, but differed markedly from the latter in possessing a cell-wall murein based on l-lysine (type l-lys-l-thr-gly). comparative 16s rrna gene sequencing showed that the unknown bacterium repr ... | 2002 | 11837304 |
| identification of lactic acid bacteria from spoiled, vacuum-packaged 'gravad' rainbow trout using ribotyping. | a total of 296 lactic acid bacteria (lab) isolated from spoiled, vacuum-packaged 'gravad' rainbow trout stored at 3 and 8 degrees c were characterised and identified using a molecular approach. the isolates were initially grouped according to their hindiii restriction endonuclease profiles and further identified to species level using an rrna gene restriction pattern (ribotype) identification database. lactobacillus sakei, l. curvatus and carnobacterium piscicola were the three main species dete ... | 2002 | 11843406 |
| the probiotic potential against vibriosis of the indigenous microflora of rainbow trout. | the antibacterial properties of the indigenous microflora of rainbow trout (oncorhynchus mykiss walbaum) and the potential use of inhibitory bacteria as fish probiotics were investigated. a total of 1018 bacteria and yeasts were isolated on tryptone soy agar (tsa) from skin, gills and intestine. forty-five of these inhibited growth of the fish pathogenic bacterium vibrio anguillarum in a well diffusion assay. the antagonism was most prominent among pseudomonas spp., as 28 (66%) of the antagonist ... | 2001 | 11846769 |
| growth of carnobacterium divergens v41 and production of biogenic amines and divercin v41 in sterile cold-smoked salmon extract at varying temperatures, nacl levels, and glucose concentrations. | a complete factorial design 2(3) was used to study some aspects of carnobacterium divergens v41 metabolism (growth, biogenic amine production, and divercin v41 production) in sterile cold-smoked salmon extract (sse) at varying temperatures (3 to 9 degrees c), nacl levels (2.5 to 6.5%), and glucose concentrations (2 to 6 g liter(-1)). the results showed that temperature and nacl content were the most influential factors on growth parameters in sse. predictive models are suggested for the assessme ... | 2002 | 11848564 |
| bacteriocin production by spray-dried lactic acid bacteria. | cell survival and antagonistic activity against listeria innocua, listeria monocytogenes and staphylococcus aureus were investigated after spray-drying three bacteriocin-producing strains of lactic acid bacteria: carnobacterium divergens, lactobacillus salivarius and lactobacillus sakei. | 2002 | 11849498 |
| sensory, chemical and bacteriological changes in vacuum-packed pressurised squid mantle (todaropsis eblanae) stored at 4 degrees c. | sensory, chemical and bacteriological changes were studied in vacuum-packed squid mantles (todaropsis eblanae) that were pressurised at 150, 200, 300 and 400 megapascal (mpa) for 15 min at ambient temperature and stored at 4 degrees c. sensory analysis showed that the higher the pressurisation the longer the shelf-life. thus, the lot pressurised at 400 mpa was rejected after 28 days' storage compared with 7 days for the untreated lot. the chemical results generally corresponded with the sensory ... | 2002 | 11929163 |
| change in the composition of the microflora on vacuum-packaged beef during chiller storage. | five fresh beefcut samples were divided into 10 pieces, respectively, vacuum-packaged and stored at 2 degrees c for up to 6 weeks. the average ph values of five pieces from five beef samples were 5.62 +/- 0.04 at the start of storage and 5.12 +/- 0.07 after 6 weeks of storage. the pieces were homogenized, diluted and cultivated at weekly intervals on glucose-blood-liver (bl), de man rogosa and sharpe (mrs) and trypticase soya (ts) agars at 7 degrees c for 10-14 days. from plates with 30-300 colo ... | 2002 | 11929174 |
| production of biogenic amines and divercin v41 in cold smoked salmon inoculated with carnobacterium divergens v41, and specific detection of this strain by multiplex-pcr. | the objective of this study was to determine the technological behaviour (implantation and biogenic amines production) of carnobacterium divergens v41, an anti-listeria bacteriocin producer (divercin v41), after inoculation in cold smoked salmon (css). | 2002 | 11966900 |
| microbial spoilage and formation of biogenic amines in fresh and thawed modified atmosphere-packed salmon (salmo salar) at 2 degrees c. | to evaluate the microbial spoilage, formation of biogenic amines and shelf life of chilled fresh and frozen/thawed salmon packed in a modified atmosphere and stored at 2 degrees c. | 2002 | 11966922 |
| role of acetate in production of an autoinducible class iia bacteriocin in carnobacterium piscicola a9b. | carnobacterium piscicola strain a9b isolated from cold smoked salmon inhibits growth of the food-borne pathogen listeria monocytogenes partly due to the production of a proteinaceous compound (l. nilsson, l. gram, and h. h. huss. j. food prot. 62:336-342, 1999). the purpose of the present study was to purify the compound and describe factors affecting its production, with particular emphasis on food-relevant factors. amino acid sequencing showed that the compound is a class iia bacteriocin with ... | 2002 | 11976095 |
| thermoadaptation of alpha-galactosidase agab1 in thermus thermophilus. | the evolutionary potential of a thermostable alpha-galactosidase, with regard to improved catalytic activity at high temperatures, was investigated by employing an in vivo selection system based on thermophilic bacteria. for this purpose, hybrid alpha-galactosidase genes of agaa and agab from bacillus stearothermophilus kve39, designated agaa1 and agab1, were cloned into an autonomously replicating thermus vector and introduced into thermus thermophilus of1053gd (deltaagat) by transformation. th ... | 2002 | 12029056 |
| development of genus/species-specific pcr analysis for identification of carnobacterium strains. | heterofermentative lactic acid bacteria belonging to the genus carnobacterium are currently divided into seven different species, c. piscicola, c. mobile, c. gallinarum, c. inhibens, c. divergens, c. funditum, and c. alterfunditum. 16s rdna-targeted pcr assay was carried out for the identification of the genus carnobacterium. in addition, type strains of all carnobacterium species were analyzed by 16s-23s rdna intergenic spacer analysis in comparison with type strains of phylogenetically related ... | 2002 | 12029523 |
| numbers and types of microorganisms in vacuum-packed cold-smoked freshwater fish at the retail level. | fifty-four packages (each one belonging to a different lot) of vacuum-packed cold-smoked salmon (30) and trout (24) produced by six spanish smokehouses were obtained at retail level after 3 weeks storage at 2+/-1 degrees c. sensorial, chemical, physicochemical and microbiological characteristics were examined. overall, ph, a(w), salt content in water phase, aerobic plate counts at 30 and 25 degrees c. levels of enterobacteriaceae, lactic acid bacteria (lab), fungi and presumptive aeromonads and ... | 2002 | 12076034 |
| characterization of carnobacterium divergens strain 6251 isolated from intestine of arctic charr (salvelinus alpinus l.). | an atypical strain of carnobacterium divergens, strain 6251, was isolated from the small intestine of arctic charr (salvelinus alpinus l.), fed high dietary carbohydrate. this strain showed marked growth inhibitory effects in vitro against the fish pathogens aeromonas salmonicida subsp. salmonicida (furunculosis), vibrio anguillarum (vibriosis) and vibrio viscocus (winter ulcer). the strain is a non-motile gram-positive psychrotrophic rod that lacks both catalase and oxidase, grows at ph 9.1 (ct ... | 2002 | 12086178 |
| mutational analysis of the role of charged residues in target-cell binding, potency and specificity of the pediocin-like bacteriocin sakacin p. | the significance of charged residues for the target-cell binding, potency and specificity of pediocin-like bacteriocins has been studied by site-directed mutagenesis of sakacin p. most of the charged residues are located in the n-terminal half, which is thought to mediate the initial binding of these bacteriocins to target cells through electrostatic interaction. all the mutated peptides in which the net positive charge was reduced by one (by replacing a charged residue with threonine) exhibited ... | 2002 | 12101290 |
| a pcr-based method for identification of lactobacilli at the genus level. | we developed a polymerase chain reaction (pcr)-based method for the identification of lactobacilli at the genus level. one specific primer, lblma1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16s and 23s rrna genes in a number of lactobacillus strains. amplification with lblma1-rev and r16-1, a universal primer, generated a pcr product for 23 lactobacillus species. electrophoresis did not reveal any discrete bands when escherichia coli, la ... | 2002 | 12351242 |
| carnobacterium viridans sp. nov., an alkaliphilic, facultative anaerobe isolated from refrigerated, vacuum-packed bologna sausage. | a facultatively anaerobic, non-spore-forming, psychrophilic, gram-positive, non-aciduric but alkaliphilic, rod-shaped bacterium (mpl-11t) was found to be responsible for green discoloration of refrigerated vacuum-packaged bologna upon opening of the package. although aerococcus viridans, which had been implicated earlier in causing the same problem, was also found, this is the first report of discoloration caused by an organism shown to be a species of carnobacterium. bacterial discoloration was ... | 2002 | 12361300 |
| differentiation of closely related carnobacterium food isolates based on 16s-23s ribosomal dna intergenic spacer region polymorphism. | a novel strategy for identification of carnobacterium food isolates based on restriction fragment length polymorphism (rflp) of pcr-amplified 16s-23s ribosomal intergenic spacer regions (isrs) was developed. pcr amplification from all carnobacterium strains studied always yielded three isr amplicons, which were designated the small isr (s-isr), the medium isr (m-isr), and the large isr (l-isr). the lengths of these isrs varied from one species to another. carnobacterium divergens ncdo 2763(t) an ... | 2002 | 12406725 |
| isolation of carnobacterium piscicola from human pus--case report. | carnobacterium piscicola was first described in 1984. these bacteria are often isolated from fish afflicted with bacterial infections. to date, there has been no reported isolation of this bacterium from human specimens. we report here the isolation of c. piscicola from the pus following traumatic amputation of the right hand in the wrist of a 35-year-old man. the traumatic amputation occurred with an industrial water sawmill. the identity of the human strain was determined biochemically, by 16s ... | 2002 | 12422528 |
| comparative studies of immunity proteins of pediocin-like bacteriocins. | genes encoding pediocin-like bacteriocins are usually co-transcribed with a gene encoding a cognate immunity protein. to investigate the functionality and specificity of immunity proteins, immunity genes belonging to the bacteriocins curvacin a, enterocin a, enterocin p, leucocin a, pediocin pa-1 and sakacin p, as well as a putative immunity gene, orfy, were expressed in three bacteriocin-sensitive lactic acid bacteria (lactobacillus sake, carnobacterium piscicola and enterococcus faecalis). the ... | 2002 | 12427956 |
| leuconostoc gasicomitatum is the dominating lactic acid bacterium in retail modified-atmosphere-packaged marinated broiler meat strips on sell-by-day. | lactic acid bacteria (lab) in retail, modified-atmosphere-packaged (map), marinated broiler meat strips on sell-by-day were mainly identified as leuconostoc gasicomitatum. a total of 32 packages, three to five packages of seven differently marinated broiler meat products, were studied at the end of the producer-defined shelf life (at 6 degrees c, 7-9 days depending on the manufacturer). prior to the microbiological analyses, appearance and smell of the product was checked and ph measured. bacter ... | 2003 | 12430775 |
| effects of environmental factors on leucine catabolism by carnobacterium piscicola. | the metabolites from leucine degradation are involved in dry fermented sausage aroma. the catabolism of leucine by a strain of carnobacterium piscicola was studied directly in the growth medium with 3h-labelled leucine to investigate the effect of five parameters: phase of growth, ph, oxygen, glucose and alpha-ketoisocaproic acid. resting cells (rc) were also incubated with 3h-labelled leucine. the radioactive metabolites from leucine catabolism were analysed by high performance liquid chromatog ... | 2003 | 12485743 |
| identification of lactic acid bacteria from spoilage associations of cooked and brined shrimps stored under modified atmosphere between 0 degrees c and 25 degrees c. | to evaluate spoilage and identify lactic acid bacteria (lab) from spoilage associations of cooked and brined shrimps stored under modified atmosphere packaging (map) at 0, 5, 8, 15 and 25 degrees c. | 2003 | 12492927 |
| immunomodulatory effects of nisin in turbot (scophthalmus maximus l.). | in the present work, the effect of nisin on the non-specific immune response of turbot (scophthalmus maximus l.) leukocytes has been studied both in vitro and in vivo. the head kidney macrophage chemiluminescent (cl) response was significantly increased with intermediate doses of nisin (2.5 and 0.025 micro g ml(-1)) whilst the higher dose (25 micro g ml(-1)) significantly decreased the response after 24h incubation. when the incubation time was extended to 72 h, significant differences between d ... | 2003 | 12526879 |
| the lactic acid bacteria: a literature survey. | the purpose of this review article on the lactic acid bacteria grew from an early curiosity and a desire to convey and impart the broad scope of literary information on their functions as starter cultures, in the manufacture of fermentation products such as dairy products and alcoholic beverages, as well as their contribution to better health. this review article is an attempt to empower the reader and to circumvent the difficult task in acquiring and elucidating a large body of information. the ... | 2002 | 12546196 |
| [a carnobacterium-like organism isolated form a patient with multiple bacterial synergistic gangrene]. | an atypical lactic-acid producing gram positive rod y6 strain was studied in this report, which was isolated form clinic sample of a patient with multiple bacterial synergistic gangrene, and could not be identified by routine method. a 1.5 kb of 16s rdna of y6 strain was synthesized and sequenced. comparative 16s rdna sequence analyses revealed that stain y6 is most closely related to the genus of carnobacterium. the overall similarity value between y6 strain and carnobacterium species are 93% t ... | 2000 | 12548873 |
| purification and characterization of brochocin a and brochocin b(10-43), a functional fragment generated by heterologous expression in carnobacterium piscicola. | brochothrix campestris atcc 43754 produces a heat-stable, two-component, nonlantibiotic, class iib bacteriocin, brochocin c (brcc), that is active against a broad range of gram-positive bacteria, including spores of clostridium botulinum. an improved purification method was developed for brcc, in which n-butanol and chloroform extraction are used. mass spectral characterization of the two components, brochocin a (brca) and brochocin b (brcb), showed that both components are excreted into the med ... | 2003 | 12620816 |
| evidence on inhibition of listeria monocytogenes by divercin v41 action. | the aim of this study was to investigate the role of divercin v41 in inhibition and prevention of listeria monocytogenes. | 2003 | 12680940 |
| effects of a bacteriocin-like inhibitory substance from carnobacterium piscicola against human and salmon isolates of listeria monocytogenes. | the aim of this study was to characterize the antagonism of a bacteriocin-like inhibitory substance (blis) produced by carnobacterium piscicola l103 against listeria monocytogenes strains isolated from salmon and human samples. the inhibitory effect of the blis was evaluated in tryptic soy agar (tsa) during different growth phases of l. monocytogenes at 5 degrees c, using the well diffusion method. also, the type of inhibition, either bacteriostatic or bactericidal of the blis in tryptic soy bro ... | 2003 | 12781946 |
| enhancement of nisin production by lactococcus lactis in periodically re-alkalized cultures. | synthesis of nisin as well as biomass production by lactococcus lactis subsp. lactis cect (colección española de cultivos tipo) 539 on both hydrolysed mussel-processing waste and whey medium were followed in three fixed volume fed-batch fermentations, with re-alkalization cycles. the two cultures on mussel-processing waste (mpw) were fed with a 240 g/l concentrated glucose and with a concentrated mpw (about 100 g of glucose/l). the culture on whey was fed with a mixture of concentrated whey (48 ... | 2003 | 12793859 |
| reclassification of lactobacillus maltaromicus (miller et al. 1974) dsm 20342(t) and dsm 20344 and carnobacterium piscicola (collins et al. 1987) dsm 20730(t) and dsm 20722 as carnobacterium maltaromaticum comb. nov. | phenotypic and genotypic characterizations of lactobacillus maltaromicus strains dsm 20342(t) and dsm 20344 provided evidence for the reclassification of this species in the genus carnobacterium. moreover, phenotypic and genotypic comparisons made between l. maltaromicus and carnobacterium piscicola highlighted that these two species should be considered synonyms. for these reasons, the species carnobacterium maltaromaticum comb. nov. (type strain dsm 20342(t) = atcc 27865(t) = ccug 30142(t) = c ... | 2003 | 12807185 |
| thermal resistances and lactate and diacetate sensitivities of bacteria causing bologna discolouration. | this report describes the effects of heat, sodium lactate, and sodium diacetate on the viabilities of weissella viridescens atcc 12706, aerococcus viridans mpl-1 and mpl-b, and carnobacterium viridans atcc baa 336. the latter three organisms were isolated from commercial product and have previously been shown to produce green discolourations in cooked cured bologna. w. viridescens was heat resistant in beef bologna (d(60 degrees c)=14.7 min) but not in apt broth. a. viridans and c. viridans were ... | 2003 | 12915033 |
| inhibition of listeria monocytogenes in cold-smoked salmon by carnobacterium piscicola cs526 isolated from frozen surimi. | strain cs526 was isolated from frozen surimi and identified as a bacteriocin producer that had strong inhibitory activity against listeria monocytogenes. strain cs526 was identified as carnobacterium piscicola by partial 16s rdna sequence similarity. the ability of this bacteriocinogenic strain and nonbacteriocinogenic c. piscicola jcm5348 to inhibit the growth of l. monocytogenes was examined in culture broth incubated at 12 degrees c and cold-smoked salmon stored at 4, 12, and 20 degrees c. l. ... | 2003 | 12929829 |
| oral administration of formalin-inactivated cells of aeromonas hydrophila a3-51 controls infection by atypical a. salmonicida in goldfish, carassius auratus (l.). | there has been increasing interest in the use of probiotics in aquaculture to control fish diseases (e.g. douillet langdon 1994; gildberg, mikkelsen, sandaker & ringo 1997; kennedy, tucker, neidic, vermeer, cooper, jarrell & sennett 1998; deschrijver & ollevier 2000; robertson, o'dowd, burrells, williams & austin 2000), and a diverse range of gram-positive (such as carnobacterium inhibens) (robertson et al. 2000) and gram-negative bacteria, including vibrio alginolyticus (garriques & arevalo 199 ... | 2003 | 12962220 |
| bacterial influences on atlantic halibut hippoglossus hippoglossus yolk-sac larval survival and start-feed response. | a bacteria-free halibut larval rearing system was used to test 20 bacterial isolates, from british halibut hatcheries, for their toxicity towards halibut yolk-sac larvae under microbially controlled conditions. the isolates tested spanned a range of genera and species (pseudoalteromonas, halomonas marina, vibrio salmonicida-like, photobacterium phosphoreum and v. splendidus species). a pathogen of turbot, scophthalmus maximus, v. anguillarum 91079, and 2 isolates from adult halibut were also inc ... | 2003 | 14598986 |
| identification of carnobacterium, lactobacillus, leuconostoc and pediococcus by rdna-based techniques. | ribosomal dna-based techniques including the analysis of profiles generated by isr amplification, isr restriction and ardra have been evaluated as molecular tools for identifying carnobacterium, lactobacillus, leuconostoc and pediococcus. they have been applied for the molecular characterization of 91 strains with the following identities: eight carnobacterium including the eight type species of the genus; 61 lactobacillus including 40 type strains out of 45 species, 13 leuconostoc, out of them ... | 2003 | 14666983 |
| the contribution of bacteriocin to inhibition of listeria monocytogenes by carnobacterium piscicola strains in cold-smoked salmon systems. | to study the importance of bacteriocin production for the antilisterial effect of a bacteriocinogenic carnobacterium piscicola strain a9b on growth of listeria monocytogenes in broth and cold-smoked salmon systems. | 2004 | 14678166 |
| generation and utilization of polyclonal antibodies to a synthetic c-terminal amino acid fragment of divercin v41, a class iia bacteriocin. | polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized c-terminal part of divercin v41 (dvnct) conjugated to the carrier protein keyhole limpet hemocyanin (klh). the sensitivity and reactivity of the dvnct-klh-generated antibodies were evaluated by enzyme-linked immunosorbent assay (elisa) using supernatant from cultures of 13 representative lactic acid bacterium strains, and specificity was confirmed by western blot analysis. anti-dvnct-klh antibodies ... | 2004 | 14711648 |
| carnocin kz213 produced by carnobacterium piscicola 213 is adsorbed onto cells during growth. its biosynthesis is regulated by temperature, ph and medium composition. | carnocin kz213 is an antilisterial bacteriocin produced by carnobacterium piscicola 213. the effects of ph and temperature were studied during batch fermentation in mrs* medium (modified mrs without ammonium citrate or sodium acetate). the optimal ph for growth is between 6 and 7. the maximum bacteriocin productivity in the supernatant occurs at ph 7. operating at controlled ph increases the volumetric activity of the free bacteriocin by 8- to 16-fold, compared with uncontrolled ph. no bacterioc ... | 2004 | 14727147 |
| observations on the succession dynamics of lactic acid bacteria populations in chill-stored vacuum-packaged beef. | drip samples were collected at 4-week intervals from 10 vacuum-packaged beef striploins stored for 16 weeks at -1.5 degrees c and assayed for populations of lactic-acid bacteria (lab), ph and spoilage-causing fermentation products. a total of 15 lab species were identified using pulsed-field gel electrophoresis and biochemical analysis. a pattern of succession was observed during storage between strains of carnobacterium, lactobacillus, leuconostoc and pediococcus. acetic acid production was ass ... | 2004 | 14751682 |
| whole-organism fingerprinting of the genus carnobacterium using fourier transform infrared spectroscopy (ft-ir). | sixty seven strains of carnobacterium, atypical lactobacillus, enterococcus durans, lactobacillus maltaromicus and vagacoccus salmoninarum were examined by fourier transform infrared (ft-ir) spectroscopy. the effects of culture age and reproducibility over a six month period were also investigated. the results were analysed by multivariate statistics and compared with those from a previous numerical phenetic study, a pyrolysis mass spectrometry (pyms) study and with investigations which used dna ... | 2004 | 15046307 |
| nmr solution structure of the precursor for carnobacteriocin b2, an antimicrobial peptide from carnobacterium piscicola. | type iia bacteriocins, which are isolated from lactic acid bacteria that are useful for food preservation, are potent antimicrobial peptides with considerable potential as therapeutic agents for gastrointestinal infections in mammals. they are ribosomally synthesized as precursors with an n-terminal leader, typically 18-24 amino acid residues in length, which is cleaved during export from the producing cell. we have chemically synthesized the full precursor of carnobacteriocin b2, precarnobacter ... | 2004 | 15096213 |
| influence of antimicrobial agents on the spoilage of a meat-based entomophage diet. | the microbial decomposition of a meat-based entomophage diet presented in parafilm packets was investigated. considerable bacteria but not fungi were associated with components used to prepare the diet (i.e., hens' eggs, liver, and ground beef). at the initial sampling time, there were no differences among diet treatments in the size of bacterial or fungal populations. bacterial populations in diets not containing antibacterial agents rapidly increased and reached an asymptote by 24 h (approxima ... | 2004 | 15154441 |
| novel expression system for large-scale production and purification of recombinant class iia bacteriocins and its application to piscicolin 126. | piscicolin 126 is a class iia bacteriocin isolated from carnobacterium piscicola jg126 that exhibits strong activity against listeria monocytogenes. the gene encoding mature piscicolin 126 (m-pisa) was cloned into an escherichia coli expression system and expressed as a thioredoxin-piscicolin 126 fusion protein that was purified by affinity chromatography. purified recombinant piscicolin 126 was obtained after cnbr cleavage of the fusion protein followed by reversed-phase chromatography. recombi ... | 2004 | 15184123 |
| heterologous expression and purification of active divercin v41, a class iia bacteriocin encoded by a synthetic gene in escherichia coli. | divercin v41, a class iia bacteriocin with strong antilisterial activity, is produced by carnobacterium divergens v41. to express a recombinant version of divercin v41, we constructed a synthetic gene that encodes the mature divercin v41 peptide and then overexpressed the gene in pet-32b by using the t7 rna polymerase promoter in the escherichia coli origami (de3)(plyss) strain. the dvnrv41 peptide was expressed as a translational fusion protein with thioredoxin and accumulated in the cell cytop ... | 2004 | 15205430 |
| simultaneous detection of carnobacterium and leuconostoc in meat products by multiplex pcr. | to develop a multiplex pcr approach for simultaneous detection of leuconostoc and carnobacterium and its validation in meat products. | 2004 | 15239706 |
| characterization of mesentericin st99, a bacteriocin produced by leuconostoc mesenteroides subsp. dextranicum st99 isolated from boza. | lactic acid bacteria isolated from boza, a cereal-fermented beverage from belogratchik, bulgaria, were screened for the production of bacteriocins. with the first screening, 13 of the 52 isolates inhibited the growth of listeria innocua and lactobacillus plantarum. the cell-free supernatant of one of these strains, classified as leuconostoc mesenteroides subsp. dextranicum st99, inhibited the growth of bacillus subtilis, enterococcus faecalis, several lactobacillus spp., lactococcus lactis subsp ... | 2004 | 15252717 |
| identification of carnobacterium species by restriction fragment length polymorphism of the 16s-23s rrna gene intergenic spacer region and species-specific pcr. | the genus carnobacterium is currently divided into the following eight species: carnobacterium piscicola, c. divergens, c. gallinarum, c. mobile, c. funditum, c. alterfunditum, c. inhibens, and c. viridans. an identification tool for the rapid differentiation of these eight carnobacterium species was developed, based on the 16s-23s ribosomal dna (rdna) intergenic spacer region (isr). pcr-restriction fragment length polymorphism (pcr-rflp) analysis of this 16s-23s rdna isr was performed in order ... | 2004 | 15294774 |
| explorative multivariate analyses of 16s rrna gene data from microbial communities in modified-atmosphere-packed salmon and coalfish. | modified-atmosphere packaging (map) of foods in combination with low-temperature storage extends product shelf life by limiting microbial growth. we investigated the microbial biodiversity of map salmon and coalfish by using an explorative approach and analyzing both the total amounts of bacteria and the microbial group composition (both aerobic and anaerobic bacteria). real-time pcr analyses revealed a surprisingly large difference in the microbial loads for the different fish samples. the micr ... | 2004 | 15294842 |
| nmr solution structure of imb2, a protein conferring immunity to antimicrobial activity of the type iia bacteriocin, carnobacteriocin b2. | bacteriocins produced by lactic acid bacteria are potent antimicrobial compounds which are active against closely related bacteria. producer strains are protected against the effects of their cognate bacteriocins by immunity proteins that are located on the same genetic locus and are coexpressed with the gene encoding the bacteriocin. several structures are available for class iia bacteriocins; however, to date, no structures are available for the corresponding immunity proteins. we report here ... | 2004 | 15362858 |
| detection and differentiation of several food-spoilage lactic acid bacteria by multiplex polymerase chain reaction, capillary gel electrophoresis, and laser-induced fluorescence. | in this work, a complete analytical procedure is investigated to differentiate several food-spoilage lactic acid bacteria. to do that, a method involving multiplex polymerase chain reaction (pcr), capillary gel electrophoresis (cge), and laser-induced fluorescence (lif) is developed. the pcr-cge-lif protocol allows the simultaneous detection and differentiation of the genera leuconostoc and carnobacterium, the nonmotile group of species within the genus carnobacterium, and the three species of t ... | 2004 | 15373396 |
| detection of low levels of listeria monocytogenes cells by using a fiber-optic immunosensor. | biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. an antibody-based fiber-optic biosensor to detect low levels of listeria monocytogenes cells following an enrichment step was developed. the principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture listeria cells on the fiber. capture of cel ... | 2004 | 15466560 |
| biodiversity of listeria monocytogenes sensitivity to bacteriocin-producing carnobacterium strains and application in sterile cold-smoked salmon. | the aim of this study was to demonstrate the inhibitory capacity of carnobacterium strains against a collection of listeria monocytogenes strains in cold-smoked salmon (css). | 2004 | 15479419 |
| purification, characterization and amino acid sequencing of divergicin m35: a novel class iia bacteriocin produced by carnobacterium divergens m35. | carnobacterium divergens m35, isolated from a commercial sample of frozen smoked mussels, produces a new bacteriocin, divergicin m35, a class iia bacteriocin. divergicin m35 is sensitive to pronase-e, alpha-chymotrypsin and proteinase k, but not to trypsin and withstands thermal treatments up to 121 degrees c for 30 min. divergicin m35 was extracted from the culture supernatant of c. divergens m35 using an sp-sepharose cation-exchange column, desalted and purified on a c18 sep-pack column and fu ... | 2004 | 15541799 |
| enterococcus species dominating in fresh modified-atmosphere-packaged, marinated broiler legs are overgrown by carnobacterium and lactobacillus species during storage at 6 degrees c. | in order to show which of the initial lactic acid bacteria (lab) contaminants are also causing spoilage of a modified-atmosphere-packaged (map), marinated broiler leg product at 6 degrees c, lab were enumerated and identified on the 2nd and 17th days following manufacture. a total of 8 fresh and 13 spoiled packages were studied for lab levels. in addition, aerobic mesophilic bacteria and enterobacteriaceae were determined. the average cfu/g values in the 8 fresh packages were 1.3 x 10(3), 9.8 x ... | 2005 | 15582737 |
| characterization and identification of lactic acid bacteria in "morcilla de burgos". | a total of 176 lactic acid bacteria (lab) isolated from a typical spanish blood sausage called "morcilla de burgos" were identified by means of phenotypic characteristics and 16s rdna rflp (ribotyping). lab were isolated from "morcilla" of different producers and in different storage periods, which includes unpackaged, vacuum and modified atmosphere packaged "morcilla" and vacuum packed and pasteurised "morcilla". the knowledge of specific spoilage bacteria of "morcilla de burgos" will be useful ... | 2005 | 15582739 |
| growth inhibition of listeria monocytogenes by a nonbacteriocinogenic carnobacterium piscicola. | this study elucidates the mechanisms by which a nonbacteriocinogenic carnobacterium piscicola inhibits growth of listeria monocytogenes. | 2005 | 15610430 |
| bacterial diversity in spent mushroom compost assessed by amplified rdna restriction analysis and sequencing of cultivated isolates. | spent mushroom compost (smc) is the residual by-product of commercial agaricus spp. cultivation, and it is mainly composed of a thermally treated cereal straw/animal manure mixture colonized by the fungal biomass. research on the valorization of this material is mainly focusing on its use as soil conditioner and plant fertilizer. an investigation of the bacterial diversity in smc was performed using molecular techniques in order to reveal the origin of smc microflora and its potential effect on ... | 2004 | 15612633 |
| purification and characterization of a novel class iia bacteriocin, piscicocin cs526, from surimi-associated carnobacterium piscicola cs526. | the bacteriocin piscicocin cs526 was inactivated by proteolytic enzymes, was stable at 100 degrees c for 30 min, had a ph range of 2 to 8, and was active against enterococcus, listeria, pediococcus, and leuconostoc. the n-terminal sequence was ygngl, not the ygngv consensus motif common in class iia bacteriocins (alternate residues underlined). the molecular mass of piscicocin cs526, which had a bactericidal mode of action, was approximately 4,430 da. | 2005 | 15640235 |
| carnobacterium pleistocenium sp. nov., a novel psychrotolerant, facultative anaerobe isolated from permafrost of the fox tunnel in alaska. | a novel, psychrotolerant, facultative anaerobe, strain ftr1t, was isolated from pleistocene ice from the permafrost tunnel in fox, alaska. gram-positive, motile, rod-shaped cells were observed with sizes 0.6-0.7 x 0.9-1.5 microm. growth occurred within the ph range 6.5-9.5 with optimum growth at ph 7.3-7.5. the temperature range for growth of the novel isolate was 0-28 degrees c and optimum growth occurred at 24 degrees c. the novel isolate does not require nacl; growth was observed between 0 an ... | 2005 | 15653921 |
| functional analysis of three plasmids from lactobacillus plantarum. | lactobacillus plantarum wcfs1 harbors three plasmids, pwcfs101, pwcfs102, and pwcfs103, with sizes of 1,917, 2,365, and 36,069 bp, respectively. the two smaller plasmids are of unknown function and contain replication genes that are likely to function via the rolling-circle replication mechanism. the host range of the pwcfs101 replicon includes lactobacillus species and lactococcus lactis, while that of the pwcfs102 replicon also includes carnobacterium maltaromaticum and bacillus subtilis. the ... | 2005 | 15746322 |
| 1.6-angstroms crystal structure of enta-im. a bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin a. | many gram-positive bacteria produce ribosomally synthesized antimicrobial peptides, often termed bacteriocins. genes encoding pediocin-like bacteriocins are generally cotranscribed with or in close vicinity to a gene encoding a cognate immunity protein that protects the bacteriocin-producer from their own bacteriocin. we present the first crystal structure of a pediocin-like immunity protein, enta-im, conferring immunity to the bacteriocin enterocin a. determination of the structure of this 103- ... | 2005 | 15753083 |
| carnobacterium divergens and carnobacterium maltaromaticum as spoilers or protective cultures in meat and seafood: phenotypic and genotypic characterization. | carnobacterium, a genus of lactic acid bacteria, frequently dominate the microflora of chilled vacuum- or modified atmosphere-packed meat and seafood. in this study carnobacterium isolates were characterized by phenotypic and molecular methods in order to investigate the association of species and intra-species groups with distinct kinds of meat and seafood. of 120 test strains, 50 originated from meat (beef and pork products, including 44 strains isolated during this study and 6 strains obtaine ... | 2005 | 15830808 |
| mode of action of piscicocin cs526 produced by carnobacterium piscicola cs526. | piscicocin cs526 is a unique class iia bacteriocin produced by carnobacterium piscicola cs526. the mode of action against the sensitive strain listeria monocytogenes iid581 was evaluated. | 2005 | 15836484 |
| specific molecular detection of carnobacterium piscicola sf668 in cold smoked salmon. | to establish a rapid and reliable multiplex pcr (mpcr)-based method allowing specific identification of carnobacterium piscicola sf668 during storage of cold smoked salmon (css). | 2005 | 15836740 |
| [prevalence of nonmevalonate and mevalonate pathways for isoprenoid biosynthesis among bacteria of different systematic groups]. | the effect of fosmidomycin and mevinoline, inhibitors of the nonmevalonate and the mevalonate pathway of isoprenoid biosynthesis, respectively, on the growth of 34 anaerobic and 10 aerobic prokaryotic strains was studied. fosmidomycin at the concentrations used was shown to inhibit the growth of 9 (of 10) representatives of the family microbacteriaceae, 4 (of 5) strains of thermoanaerobacter, and 11 (of 12) strains of clostridium, whereas mevinoline inhibited the growth of lactobacilli (carnobac ... | 2005 | 15938394 |
| shelf life and safety aspects of chilled cooked and peeled shrimps (pandalus borealis) in modified atmosphere packaging. | to evaluate the growth of listeria monocytogenes and shelf life of cooked and peeled shrimps in modified atmosphere packaging (map). | 2005 | 15960666 |
| effect of inoculation of carnobacterium divergens v41, a bio-preservative strain against listeria monocytogenes risk, on the microbiological, chemical and sensory quality of cold-smoked salmon. | the aim of this study was to develop a bio-preservation strategy for cold-smoked salmon (css) by the use of lactic acid bacteria previously selected for their capability to inhibit the growth of listeria monocytogenes in the product. the spoiling potential of three carnobacterium strains (carnobacterium divergens v41, carnobacterium piscicola v1 and sf668) was tested in sterile css blocks inoculated by 10(4-5) cfu g(-)(1) and stored under vacuum for 9 days at 4 degrees c followed by 19 days at 8 ... | 2005 | 15979753 |
| quantitative polymerase chain reaction used for the rapid detection of carnobacterium species from french soft cheeses. | an identification method by pcr, specific to the carnobacterium genus, was optimised by testing it on 28 bacterial strains. primers from the literature were tested to differentiate carnobacterium strains present among various bacterial species. the dna of carnobacterium species (c. alterfunditum, c. divergens, c. funditum, c. gallinarum, c. inhibens, c. maltaromaticum, c. mobile, c. viridans), specifically amplified by the cb1-cb2r primer couple at a hybridization temperature of 69 degrees c, ga ... | 2005 | 16009510 |
| antilisterial activity of a carnobacterium piscicola isolated from brazilian smoked fish (surubim [pseudoplatystoma sp.]) and its activity against a persistent strain of listeria monocytogenes isolated from surubim. | data on the prevalence and growth of listeria monocytogenes in lightly preserved fish products from subtropical and tropical regions are very scarce. our research describes l. monocytogenes that was detected in 5% of the packages of cold-smoked surubim, a native brazilian freshwater fish that we analyzed, and shows that the strains isolated were of the same random amplified polymorphic dna subtype as the strains that were isolated from the same factory 4 years earlier. a bacteriocinogenic strain ... | 2005 | 16245709 |
| [bacterial 16s rdna sequence analysis of siberian tiger faecal flora]. | bacterial 16s rdna library of siberian tiger was developed and 15 different clones were obtained using ecor i and hind iii in restriction fragment length polymorphism analysis. dna sequencing and similarity analysis showed that 10 clones matched corresponding clostridium sequences, of which 6 sequences had over 99% similarity with clostridium novyi type a, and 4 sequences had 97% similarity with swine manure bacterium rt-18b, which identified as peptostreptococcus spp. the other five 16s rdna se ... | 2005 | 16342753 |
| characterization of the theta-type plasmid pcd3.4 from carnobacterium divergens, and modulation of its host range by repa mutation. | the complete nucleotide sequence of the 3475 bp plasmid pcd3.4 from carnobacterium divergens lv13, which encodes the bacteriocin divergicin a, was determined. nucleotide sequence, deletion and complementation analyses revealed the presence of a trans-acting replication protein, repa, and dna sequences involved in plasmid replication and copy-number control. the dna region preceding the repa gene probably contains the origin of replication. this sequence includes four and a half direct repeats (i ... | 2006 | 16385127 |
| multiple characterizations of listeria monocytogenes sensitive and insensitive variants to divergicin m35, a new pediocin-like bacteriocin. | divergicin m35 is a new class iia bacteriocin produced by carnobacterium divergicin m35. the bactericidal activity of this antimicrobial peptide was tested against a set of 11 strains of listeria monocytogenes isolated from food. | 2006 | 16405682 |
| random amplification of polymorphic dna (rapd) typing of carnobacteria isolated from hindgut chamber and large intestine of atlantic cod (gadus morhua l.). | autochthonous and allochthonous bacteria were isolated from hindgut chamber and large intestine of fed and starved atlantic cod (gadus morhua l.). all bacterial strains isolated from hindgut chamber belong to carnobacteria. however, only 10.2% of the bacteria strains from the large intestine belong to carnobacteria. random amplification of polymorphic dna (rapd) analysis using three selective primers, was performed to further identify the carnobacteria strains. nine of these were isolated from h ... | 2006 | 16464694 |
| innate immune responses in rainbow trout (oncorhynchus mykiss, walbaum) induced by probiotics. | carnobacterium maltaromaticum b26 and carnobacterium divergens b33, which were isolated from the intestine of healthy rainbow trout (oncorhynchus mykiss, walbaum), were selected as being potentially useful as probiotics with effectiveness against aeromonas salmonicida and yersinia ruckeri. thus, rainbow trout administered with feed supplemented with b26 or b33 dosed at >10(7) cells g(-1) feed conferred protection against challenge with virulent cultures of the pathogens. moreover, both cultures ... | 2006 | 16631379 |
| changes in the spoilage-related microbiota of beef during refrigerated storage under different packaging conditions. | the microbial spoilage of beef was monitored during storage at 5 degrees c under three different conditions of modified-atmosphere packaging (map): (i) air (map1), (ii) 60% o2 and 40% co2 (map2), and (iii) 20% o2 and 40% co2 (map3). pseudomonas, enterobacteriaceae, brochothrix thermosphacta, and lactic acid bacteria were monitored by viable counts and pcr-denaturing gradient gel electrophoresis (dgge) analysis during 14 days of storage. moreover, headspace gas composition, weight loss, and beef ... | 2006 | 16820458 |
| eggshell factors influencing eggshell penetration and whole egg contamination by different bacteria, including salmonella enteritidis. | trans-shell infection routes and whole egg contamination of 7 selected bacterial strains; staphylococcus warneri, acinetobacter baumannii, alcaligenes sp., serratia marcescens, carnobacterium sp., pseudomonas sp. and salmonella enteritidis, recovered from egg contents, were studied. the first objective was to correlate bacterial eggshell penetration with various eggshell characteristics and bacterial strains. an agar approach was used to assess the eggshell penetration. the second objective was ... | 2006 | 16822571 |
| production of piscicolin 126 by carnobacterium maltaromaticum ual26 is controlled by temperature and induction peptide concentration. | carnobacterium maltaromaticum ual26 produces the antimicrobial peptides (bacteriocins) piscicolin 126, first isolated from c. maltaromaticum jg126, and carnobacteriocin bm1, first isolated from c. maltaromaticum lv17. c. maltaromaticum ual26 is especially inhibitory to strains of listeria monocytogenes. bacteriocin activity is not observable in the supernatant of cultures of ual26 grown in liquid media at 25 degrees c, but at temperatures less than 19 degrees c bacteriocin activity can be detect ... | 2006 | 16927067 |
| inhibition of listeria innocua growth by antimicrobial-producing lactic acid cultures in vacuum-packed cold-smoked salmon. | the biopreservative potential of three antimicrobial-producing lactic acid bacteria strains was evaluated on cold-smoked salmon. lactobacillus casei, lactobacillus plantarum and carnobacterium piscicola were added singly or in association to cold-smoked salmon, artificially contaminated with listeria innocua and stored under vacuum for 30 days at 4 degrees c. all the lactic cultures were able to inhibit listeria innocua growth, showing a bacteriostatic or bactericidal effect, without affecting n ... | 2006 | 16943070 |
| effect of bacterial interactions on the spoilage of cold-smoked salmon. | cold-smoked salmon is a lightly preserved fish product in which a mixed microbial flora develops during storage and where the interactive behaviour of micro-organisms may contribute to their growth and spoilage activity. the aim of this study was to assess the effect of the bacterial interactions between the main species contaminating the cold-smoked salmon on bacterial growth, chemical and sensory changes, and spoilage. first, carnobacterium piscicola, photobacterium phosphoreum, lactobacillus ... | 2006 | 16949172 |
| cytokine expression in leucocytes and gut cells of rainbow trout, oncorhynchus mykiss walbaum, induced by probiotics. | understanding how the various host cells respond to probiotic bacteria in vitro may provide important insight into elaborate immune responses triggered by beneficial bacteria. the aim of this study was to investigate the detailed pattern of the mrna expression of cytokines (il-1beta, il-8, tnf-alpha and tgf-beta) in head kidney (hk) leucocytes and gut cells isolated from rainbow trout (oncorhynchus mykiss walbaum) after co-culturing with live probiotics. hk leucocytes and gut cells adjusted to 5 ... | 2006 | 17011045 |
| functional characterization of a three-component regulatory system involved in quorum sensing-based regulation of peptide antibiotic production in carnobacterium maltaromaticum. | quorum sensing is a form of cell-to-cell communication that allows bacteria to control a wide range of physiological processes in a population density-dependent manner. production of peptide antibiotics is one of the processes regulated by quorum sensing in several species of gram-positive bacteria, including strains of carnobacterium maltaromaticum. this bacterium and its peptide antibiotics are of interest due to their potential applications in food preservation. the molecular bases of the quo ... | 2006 | 17054797 |
| histological changes in intestine of atlantic salmon (salmo salar l.) following in vitro exposure to pathogenic and probiotic bacterial strains. | furunculosis and vibriosis are diseases that cause severe economic losses in the fish-farming industry. the foregut of the atlantic salmon (salmo salar l.) was exposed in vitro to two fish pathogens, aeromonas salmonicida (causative agent of furunculosis) and vibrio anguillarum (causative agent of vibriosis), and to one probiotic strain, carnobacterium divergens, at 6 x 10(4) or 6 x 10(6) viable bacteria per milliliter. histological changes following bacterial exposure were assessed by light and ... | 2007 | 17120052 |
| role of broiler carcasses and processing plant air in contamination of modified-atmosphere-packaged broiler products with psychrotrophic lactic acid bacteria. | some psychrotrophic lactic acid bacteria (lab) are specific meat spoilage organisms in modified-atmosphere-packaged (map), cold-stored meat products. to determine if incoming broilers or the production plant environment is a source of spoilage lab, a total of 86, 122, and 447 lab isolates from broiler carcasses, production plant air, and map broiler products, respectively, were characterized using a library of hindiii restriction fragment length polymorphism (rflp) patterns of the 16 and 23s rrn ... | 2007 | 17142357 |
| a selective enumeration medium for carnobacterium maltaromaticum. | a selective medium was proposed for isolating the species carnobacterium maltaromaticum from cheeses. this medium, named cm, was elaborated using combinations of three antibiotics (gentamicin, nalidixic acid, vancomycin) and alkaline ph value (8.8). an experimental design (doehlert matrix) was drawn up to optimize the experimental conditions of the preparation of the medium. based on the ts-ye agar medium, it contained 3.5 mg l(-1) of vancomycin, 5.0 mg l(-1) of gentamicin, and 20 mg l(-1) of na ... | 2007 | 17145089 |
| sequencing of variable regions of the 16s rrna gene for identification of lactic acid bacteria isolated from the intestinal microbiota of healthy salmonids. | the aim of this study was to identify lactic acid bacteria (lab) using polymerase chain reaction (pcr) amplification of variable regions of the 16s rrna gene. thirteen lab strains were isolated from the intestinal microbiota of healthy salmonids. a approximately 500-bp region of the highly conserved 16s rrna gene was pcr-amplified and following this, a portion of the amplicon (272-bp) including the v1 and v2 variable regions was sequenced. the sequence containing both the v1 and v2 region provid ... | 2007 | 17239438 |
| lactic acid bacteria associated with vacuum-packed cooked meat product spoilage: population analysis by rdna-based methods. | to determine the lactic acid bacteria (lab) implicated in bloating spoilage of vacuum-packed and refrigerated meat products. | 2007 | 17241356 |