Publications

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cellulosimicrobium aquatile sp. nov., isolated from panagal reservoir, nalgonda, india.a gram-stain positive, non-spore forming, non-motile, yellow-pigmented, rod shaped and coccoid aerobic strain designated 3bp(t) was isolated from panagal reservoir, at nalgonda, telangana, india. based on 16s rrna gene sequence analysis it was identified that strain 3bp(t) belongs to the class actinobacteria and is closely related to cellulosimicrobium funkei vtt e-072700(t), cellulosimicrobium cellulans lmg16221(t) and cellulosimicrobium terreum kctc 19206(t). the dna-dna relatedness data demon ...201526373417
functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel.cellulosome is a kind of multienzyme complex that displays high activity, selectivity, and stability. here, we report a novel, non-cellulolytic, cellulosome-like multienzyme complex that produced by the cellulosimicrobium cellulans wild-type strain f16 isolated from soil microflora. this multienzyme complex, with excellent catalytic efficiency of kcat 13.2 s(-1) to remove the c-7 xylosyl group from 7-xylosyl-10-deacetylpaclitaxel (10-daxp), has an outstanding tolerance against organic solvents a ...201526347949
modification in the properties of paper by using cellulase-free xylanase produced from alkalophilic cellulosimicrobium cellulans ckmx1 in biobleaching of wheat straw pulp.alkalophilic cellulosimicrobium cellulans ckmx1 isolated from mushroom compost is an actinomycete that produces industrially important and environmentally safer thermostable cellulase-free xylanase, which is used in the pulp and paper industry as an alternative to the use of toxic chlorinated compounds. strain ckmx1 was previously characterized by metabolic fingerprinting, whole-cell fatty acids methyl ester analysis, and 16s rdna and was found to be c. cellulans ckmx1. crude enzyme (1027.65 u/g ...201526220821
enzymatic hydrolysis of 7-xylosyltaxanes by an extracellular xylosidase from cellulosimicrobium cellulans.to find extracellular biocatalysts that can specifically and efficiently remove the c-7 xylosyl group from 7-xylosyltaxanes.201526026963
capd peritonitis caused by co-infection with cellulosimicrobium cellulans (oerskovia xanthineolytica) and enterobacter cloacae: a case report and literature review.a 50-year-old woman with end-stage renal disease on continuous ambulatory peritoneal dialysis was admitted with abdominal pain, fever and cloudy peritoneal fluid. the diagnosis was peritonitis, and the causative bacteria were cellulosimicrobium cellulans and enterobacter cloacae. she was subsequently treated with the administration of intraperitoneal antibiotics and removal of the infected indwelling catheter. we herein report a case of cellulosimicrobium cellulans and enterobacter cloacae co-in ...201525786454
improvement for enhanced xylanase production by cellulosimicrobium cellulans ckmx1 using central composite design of response surface methodology.the effects of yeast extract (x1), nh4no3 (x2), peptone (x3), urea (x4), cmc (x5), tween 20 (x6), mgso4 (x7), and caco3 (x8) on production of xylanase from cellulosimicrobium cellulans ckmx1 were optimized by statistical analysis using response surface methodology (rsm). the rsm was used to optimize xylanase production by implementing the central composite design. statistical analysis of the results showed that the linear, interaction and quadric terms of these variables had significant effects. ...201528324413
purification and characterization of cellulase-free low molecular weight endo β-1,4 xylanase from an alkalophilic cellulosimicrobium cellulans ckmx1 isolated from mushroom compost.alkalophilic cellulosimicrobium cellulans ckmx1 isolated from mushroom compost is first report on actinomycetes that has the ability to produce thermostable cellulase-free xylanase, which is an important industrial enzyme used in the pulp and paper industry. strain ckmx1 was characterized by metabolic fingerprinting, whole-cell fatty acids methyl ester analysis and 16sr dna and found to be c. cellulans ckmx1.the enzyme was purified by gel permeation and anion exchange chromatography and had a mo ...201424908422
recombinant arthrobacter β-1, 3-glucanase as a potential effector molecule for paratransgenic control of chagas disease.chagas disease is most often transmitted to humans by trypanosoma cruzi infected triatomine bugs, and remains a significant cause of morbidity and mortality in central and south america. control of chagas disease has relied mainly on vector eradication. however, development of insect resistance has prompted us to develop a paratransgenic strategy to control vectorial transmission of t. cruzi. here, the potential role of recombinant endoglucanases as anti-trypanosomal agents for paratransgenic ap ...201323497594
production, purification and application of extracellular chitinase from cellulosimicrobium cellulans 191.this study concerned the production, purification and application of extracellular chitinase from cellulosimicrobium cellulans strain 191. in shaken flasks the maximum yield of chitinase was 6.9 u/ml after 72 h of cultivation at 25°c and 200 rpm. in a 5 l fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 u/ml after 168 h of fermentation at 25°c and 200 rpm, and using 3 vvm, it was 4.38 u/ml after 144 h of fermentation. the chitinase (61 kda) was purified about 6.65 times by se ...200924031407
production of alkaline protease from cellulosimicrobium cellulans.cellulosimicrobium cellulans is one of the microorganisms that produces a wide variety of yeast cell wall-degrading enzymes, β-1,3-glucanase, protease and chitinase. dried cells of saccharomyces cerevisiae were used as carbon and nitrogen source for cell growth and protease production. the medium components kh2po4, koh and dried yeast cells showed a significant effect (p<0.05) on the factorial fractional design. a second design was prepared using two factors: ph and percentage of dried yeast cel ...200924031317
lignocellulolytic enzyme production from submerged fermentation of paddy straw.five strains of cellulolytic bacteria and four strains of phanerochaete chrysosporium were evaluated for the lignocellulolytic enzyme production during submerged fermentation (smf) of paddy straw. extra-cellular enzyme assay for cmcase, fpase, cellobiase, xylanase, lignin peroxidase and laccase enzymes was performed after 7 and 15 days of submerged fermentation. cellulomonas cellulans mtcc 23, cytophaga hutchinsonii ncim 2338 and phanerochaete chrysosporium mtcc 787 were found to produce higher ...200723100663
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