| carbohydrate metabolism in bifidobacterium bifidum. | | 1967 | 6048259 |
| [h-nmr spectroscopy. specificity of microbial sialidases against complex substrates]. | the specificities of one viral and five bacterial sialidases were investigated by 1h-nmr-spectroscopy with substrates or substrate mixtures containing two sialic acid residues of different linkage types. this technique allows - in contrast to the methods used before - the simultaneous determination of the rates of hydrolysis of both neuac linkages in a single experiment. the substrate specificities of the enzymes are discussed on the basis of the relation of the rate constants k/k'. the data obt ... | 1984 | 6096253 |
| considerations on the value of freeze-etching technique in studying the ultrastructure of some anaerobic bacteria. | samples from periodic cultures of clostridium perfringens, clostridium oedematiens, clostridium tetani and bifidobacterium bifidum grown in liquid nutrient media were studied after freeze-fracturing and etching without pretreatment by means of chemical agents including cryo-protectants. subunits in the cell all of cl. tetani and differences in the distribution of globular particles in the plasma membrane of the investigated bacteria are revealed. | 1981 | 6261293 |
| structure of the lipoteichoic acids from bifidobacterium bifidum spp. pennsylvanicum. | the lipoteichoic acids from bifidobacterium bifidum spp. pennsylvanicum were extracted from cytoplasmic membranes or from disintegrated bacteria with aqueous phenol and purified by gel chromatography. the lipoteichoic acid preparations contained phosphate, glycerol, galactose, glucose and fatty acids in a molar ratio of 1.0:1.0:1.3:1.2:0.3. chemical analysis and nmr studies of the native preparations and of products from various acid and alkaline hydrolysis procedures gave evidence for the struc ... | 1984 | 6477947 |
| iron uptake by the microaerophilic anaerobe bifidobacterium bifidum var. pennsylvanicus. | a system was designed to investigate ferrous iron transport into bifidobacterium bifidum var. pennsylvanicus. it involved the incubation of the organisms with labeled ferrous iron in the norris medium at ph 5, in which the bacteria had grown. iron uptakes were similar under aerobic and anaerobic conditions. ferrous but not ferric iron was taken up by the organisms. iron uptake showed saturation kinetics and a marked temperature dependence. 2,4-dinitrophenol and thenoltrifluoroacetate but not azi ... | 1984 | 6518721 |
| effect of various metals and calcium metabolism inhibitors on the growth of bifidobacterium bifidum var. pennsylvanicus. | in view of the facts that the normal intestinal flora exerts beneficial effects and that bifidobacteria are a more important component in the breast-fed than in the bottle-fed infant, factors affecting the growth of the latter microorganisms are of interest. a series of transition and other metals were shown to be growth inhibitors of bifidobacterium bifidum var. pennsylvanicus. such inhibition could be reversed fully or partially by 0.5-1.0 mm fe2+ in the case of zn2+, cu2+, au3+, pt4+, la3+, c ... | 1984 | 6694042 |
| [treatment of enterocolitis and other intestinal disorders with a bifidobacterium bifidum and lactobacillus acidophilus combination]. | | 1980 | 6778648 |
| [induced colonization of bifidobacterium bifidum and lactobacillus acidophilus in hospitalized newborn infants]. | | 1982 | 6808329 |
| high performance liquid chromatographic determination of bifidobacterium bifidum growth factors in human milk. | an isocratic high performance liquid chromatography (hplc) method has been developed for the determination of bifidobacterium bifidum growth factors in human milk. the method involves the gradual addition of 3 volumes of ethanol to the milk sample, filtration, and analysis of the growth factors in the filtrate by hplc. the hplc system consisted of a carbohydrate analysis column, a water-acetonitrile (70 + 30) solvent system, a flow rate of 1.0 ml/min, and a refractive index detector. the method ... | 1983 | 6826499 |
| selective localization and growth of bifidobacterium bifidum in mouse tumors following intravenous administration. | a strain of domestic bacteria, bifidobacterium bifidum (lac b), which is nonpathogenic and anerobic, selectively localized and proliferated in several types of mouse tumors following i.v. administration. none of the same bacilli could be detected in the tissues of healthy organs such as the liver, spleen, kidney, lung, blood, bone marrow, and muscle 48 or 96 hr after i.v. administration into tumor-bearing mice. proliferation of lac b in the tumor was artifically stimulated by i.p. administration ... | 1980 | 6989495 |
| [interaction between bifidobacterium bifidum, proteus vulgaris, and klebsiella pneumoniae 204 in the gastrointestinal tract of gnotobiotic chicks]. | experiments on gnotobiont chickens indicated that the strains b. bifidum 1/85 phi, p. vulgaris f-30 and k. pneumoniae 204, when introduced simultaneously into the gastrointestinal tract in a single administration, proliferate there with the pronounced predominance of bifidobacteria. 6 additional administrations of b. bifidum 1/85 phi culture resulted in the complete suppression of microorganisms belonging to the genera rroteus and klebsiella as early as 10 days after the introduction of bifidoba ... | 1981 | 7018135 |
| purification and certain properties of a bacteriocin from streptococcus mutans. | an inhibition factor from streptococcus mutans strain c3603 (serotype c) was purified and isolated, and its properties indicated that it was a bacteriocin. bacteriocin c3603 is a basic protein with a pi value of 10 and a molecular weight of 4,800. the activity of this bacteriocin was not affected by ph over a range of 1.0 to 12.0 or by storage at 100 degrees c for 10 min at ph 2.0 to 7.0 or storage at 121 degrees c for 15 min at ph 4.0. pronase; papain, phospholipase c, trypsin, and alpha-amylas ... | 1982 | 7068219 |
| influence of breast-feeding on the bifid flora of the newborn intestine. | because of the predominance of bifidobacterium bifidum in the intestine of the breast-fed infant, growth promoting factors were sought in human milk. in vitro, studies showed the presence of specific growth factors for b. bifidum in human milk. other milks, including cow's milk, sheep's milk, pig's milk, and infant formulas did not promote the growth of this species, but did show activity on bifidobacterium infantis and bifidobacterium longum. | 1980 | 7192050 |
| [studies on intestinal absorption of cellular constituents of bifidobacterium. 1. absorption of radioactivity of (3h) leucine-labeled bifidobacterium bifidum cells and subcellular materials in germfree and conventional mice (author's transl)]. | | 1980 | 7277697 |
| inability of lactobacillus casei strain gg, l. acidophilus, and bifidobacterium bifidum to degrade intestinal mucus glycoproteins. | lactic acid bacteria have been suggested for use in the prevention of relapse of ulcerative colitis and of recurrent pouchitis. these strains may not damage the protective intestinal mucus glycoproteins. | 1995 | 7481531 |
| in vitro fructooligosaccharide utilization and inhibition of salmonella spp. by selected bacteria. | in vitro experiments were conducted to determine: 1) inhibitory capacities of potential direct-fed microbial bacteria against salmonella serotypes; and 2) the ability of bifidobacterium bifidum, enterococcus faecium, lactobacillus casei, lactococcus lactis, pediococcus sp., and salmonella spp. to grow in media containing fructooligosaccharides (fos-50 or fos pure formulation) as the only carbohydrate source. thirteen bacteria (two strains of bacillus coagulans, bacillus licheniformis, bacillus s ... | 1995 | 7501585 |
| [antimutagenic action of bacterial culture liquid on mutagenesis induced by 2-nitrofluorene in salmonella typhimurium strains]. | it was shown that cell extracts and cells of streptococcus faecalis decrease the mutagenic effect of 2-nitrofluorene in salmonella typhimurium strain ta1538 by 73 and 48%, respectively. cell extracts and cells of bifidobacterium bifidum and propionibacterium shermanii exhibited weak antimutagenic activity. no antimutagenic effect was found in escherichia coli ab1157, lactobacillus delbrueckii, or streptococcus thermophilus. antimutageneicity of the cell extract of str. faecalis is both associate ... | 1995 | 7590202 |
| [architectonics of bifidobacteria populations: submicroscopic aspect of cell cohesion in bifidobacterium adolescentis and bifidobacterium bifidum]. | populations (cultures) of bifidobacterium adolescents and b. bifidum, growing on artificial liquid and agar media, are presented by highly ordered mycelial structures. the topography of them depends on mutual arrangement of polymorphic cells and the way of their daughter cells separation after division. evidences obtained by scanning electron microscopy (sem) of total preparations and by transmission electron microscopy (tem) of ultrathin sections correlate well. these data showed the existence ... | 1995 | 7616878 |
| [antimutagenic action of bacteria on mutagenesis induced by 4-nitroquinoline-1-oxide in salmonella typhimurium]. | it is shown that in the presence of culture liquid and cells of propionibacterium shermanii, p. pentosaceum, p. thoenii, p. coccoides, p. acnes, lactobacillus delbrueckii, streptococcus thermophilus, str. faecalis, bifidobacterium bifidum, and escherichia coli ab 1157 the mutagenicity of 4-nitroquinoline-1-oxide (4-nqo) in salmonella typhimurium ta 100 decreases significantly. the highest antimutagenic (desmutagenic) activity was found in culture liquid from all strains tested in the logarithmic ... | 1995 | 7616879 |
| viability and enzymatic activity of bifidobacteria in milk. | bifidobacterium breve ncfb 2258, bifidobacterium bifidum ncfb 2715, bifidobacterium longum atcc 15707, bifidobacterium angulatum atcc 27535, and lactobacillus acidophilus n2 were evaluated for viability and beta-galactosidase and alpha-galactosidase activities under conditions of refrigerated and frozen storage in reconstituted ndm. beta- and alpha-galactosidase activities were variable. bifidobacterium angulatum 27535 exhibited much greater enzymatic activities than those of the other strains. ... | 1995 | 7745146 |
| immunomodulation of human blood cells following the ingestion of lactic acid bacteria. | because of the lack of data that convincingly show immunomodulatory properties of lactic acid bacteria in humans, a study was performed in which healthy volunteers were divided into two groups and given a fermented milk product supplemented with lactobacillus acidophilus strain la1 or bifidobacterium bifidum strain bb 12 for 3 wk. blood was sampled throughout the study to assess changes in lymphocyte subsets or leukocyte phagocytic activity following consumption of the fermented products. no mod ... | 1995 | 7782506 |
| absence of cholic acid 7 alpha-dehydroxylase activity in the strains of lactobacillus and bifidobacterium. | to investigate the presence of 7 alpha-dehydroxylase activity on bile acids in the bacterial strains of fermented milk products, 46 strains of lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus acidophilus, lactobacillus gasseri, bifidobacterium adolescentis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, lactococcus lactis spp. lactis, and streptococcus salivarius spp. thermophilus were tested for their ability ... | 1994 | 7814703 |
| anti-ulcer effects of lactic acid bacteria and their cell wall polysaccharides. | the anti-ulcer effects of bifidobacteria, lactobacilli and streptococci were examined using the acetic acid-induced gastric ulcer and ethanol-induced erosion models in rats. bifidobacterium breve yit4014 and 4043, and bifidobacterium bifidum yit4007 were administered orally, and anti-ulcer effects were confirmed for not only these organisms but also their polysaccharide fractions (psfs). the major component of these anti-ulcer polysaccharides was rhamnose. in particular, polysaccharides in which ... | 1994 | 7820099 |
| growth and viability of bifidobacterium bifidum in cheddar cheese. | bifidobacterium bifidum (atcc 15696) was grown in mrs broth containing cysteine.hcl at 37 degrees c, and the cells were harvested by centrifuging at 1300 x g for 15 min at 4 degrees c. equal volumes of the cell slurry and a 2.5% solution of kappa-carrageenan were mixed and transferred by drops into a solution of .3 m kcl at 20 degrees c under an atmosphere of nitrogen. the gelled beads were separated, frozen, and lyophilized immediately. this preparation and a commercial powder preparation were ... | 1994 | 7836576 |
| feeding of bifidobacterium bifidum and streptococcus thermophilus to infants in hospital for prevention of diarrhoea and shedding of rotavirus. | acute diarrhoea is a serious cause of infant morbidity and mortality, and the development of preventive measures remains an important goal. bifidobacteria (which constitute the predominant intestinal flora of breastfed infants), as well as other lactic-acid-producing organisms such as streptococcus thermophilus, are thought to have a protective effect against acute diarrhoeal disease. however, their efficacy has not been assessed in controlled trials. in a double-blind, placebo-controlled trial, ... | 1994 | 7934445 |
| effectiveness of bifidobacterium bifidum in mediating the clinical course of murine rotavirus diarrhea. | human bifidobacterium sp strain bifidum (b. bifidum) was administered to balb/c lactating mice (n = 58) and their litters (n = 327 pups) to evaluate the ingested strain's adherent properties and ability to inhibit murine rotavirus (mrv) infection. elisa and anaerobic bacteriologic techniques were used to measure mrv shedding and colonization of bifidobacterium in the small intestine. at 13-16 d gestation, pregnant dams (and their expected litters) were randomly assigned to one of four experiment ... | 1994 | 7936820 |
| relationship between intestinal colonization of bifidobacterium bifidum in infants and the presence of exogenous and endogenous growth-promoting factors in their stools. | the relationship between the intestinal colonization of a test strain of bifidobacterium bifidum requiring human milk growth-promoting factors in vitro and the presence of growth-promoting factors either in the stools of human neonates or in their diet was investigated. thirty-one infants were inoculated with a single dose of this strain within the first 8 d of life. spores of a strictly thermophilic bacillus admixed with the b. bifidum inoculum were used as transit marker, and the fecal populat ... | 1994 | 7936821 |
| reduction of virus shedding by b. bifidum in experimentally induced mrv infection. statistical application for elisa. | the protective effect of a human strain of bifidobacterium bifidum (b. bifidum) against murine group a rotavirus (mrv) was examined in the intestines of balb/c infected mice. in experiments designed to determine whether b. bifidum mediated mrv shedding during diarrheal disease, pregnant dams (and their expected litters) were randomly assigned to the following groups: (1) mice infected with mrv alone; (2) b. bifidum-treated + mrv-infected mice; (3) b. bifidum-treated controls; and (4) saline cont ... | 1994 | 7956600 |
| [the manifestation of functional heterogeneity of the peptidoglycans in opsonophagocytic reactions]. | the peptidoglycan activity of staphylococcus aureus ccm 885 and bifidobacterium bifidum 1 was studied in the opsonophagocytic system on the basis of the luminol-dependent chemiluminescence of human neutrophils. b. bifidum peptidoglycan was more active in direct interaction with neutrophils. after treatment with normal human serum s. aureus peptidoglycan showed more pronounced neutrophil-stimulating properties. this phenomenon was in accord with a higher content of igg antibodies to s. aureus pep ... | 1993 | 8059563 |
| effectiveness of bifidobacterium bifidum in experimentally induced mrv infection: dietary implications in formulas for newborns. | the protective effect of a human strain of bifidobacterium bifidum (b. bifidum) against murine group a rotavirus (mrv) was examined in the intestines of balb/c infected mice. in experiments designed to determine whether b. bifidum mediated mrv shedding during diarrheal disease, pregnant dams (and their expected litters) were randomly assigned to the following groups: 1. mice infected with mrv alone; 2. b. bifidum treated + mrv infected mice; 3. b. bifidum treated controls; 4. saline control anim ... | 1993 | 8068900 |
| [development of a method for assessing viable bifidobacterium in the preparation bifikol]. | the method has been developed for accounting viable bifidobacteria in the complex preparation biphicol using the growth inhibitor of escherichia coli (0.01% solution of sodium azide), added to the growing medium. choice of sodium azide concentration inhibiting growth of escherichia coli m-17 and not affecting the growth of bifidobacterium bifidum 1 is described in detail. data are presented on the quality of commercial preparation made at different enterprises. | 1993 | 8162200 |
| effect of lactic acid bacteria on the intestinal production of lactate and short-chain fatty acids, and the absorption of lactose. | the characteristic fermentation pattern seen with specific saccharides in incubations with pure cultures of lactobacillus acidophilus and bifidobacterium bifidum disappeared when incubated in 16.7% fecal homogenate. the productions of lactate and short-chain fatty acids in mixed bacterial-fecal incubations were similar to productions in fecal homogenates without l acidophilus and b bifidum and were mainly associated with the specific mono-, di-, and polysaccharides added to the incubate. b bifid ... | 1994 | 8279407 |
| influence of intestinal bacterial flora on cytokine (il-1, il-6 and tnf-alpha) production by mouse peritoneal macrophages. | the effect of bacterial flora on cytokine production from resident peritoneal macrophages was investigated in the mouse. the production of il-1, il-6 and tnf-alpha was determined in germ-free, and "conventionalized" mice, as well as in monoxenic mice implanted with either the gram-negative bacterium e. coli, or the gram-positive organism bifidobacterium bifidum. macrophages from the "conventionalized" mice produced significantly more il-1 and il-6 in vitro than those of the germ-free mice. il-1 ... | 1993 | 8318673 |
| microbial purification technique of mineral dressing plants reject waters. | earlier investigations on laboratory and pilot plant scale have shown the resort to microorganisms to be a practicable approach to the problem of purifying mineral dressing plant reject waters from residual flotation reagents and/or metal ions. in spite of the proven effectiveness of this method, one major drawback, namely the pathogenicity of some microorganisms, has so far hampered its application on a commercial scale. a research programme, aimed at developing a microbial reject water purific ... | 1993 | 8357615 |
| analysis of intestinal flora of a patient with congenital absence of the portal vein. | a 14-year-old female patient, admitted for a closer examination of liver tumour (hepatocellular adenoma), was diagnosed as having a congenital absence of the portal vein. the blood ammonia level (approximately 120 micrograms dl-1) in the superior mesenteric vein was markedly low compared to the normal value of 300-350 micrograms dl-1 in the portal vein. the decreased ammonia concentration and urease activity of the patient's faeces were demonstrated. the dominant intestinal flora in the faeces o ... | 1993 | 8364525 |
| growth characteristics of bifidobacteria in ultrafiltered milk. | five replicates of raw skim milk were ultrafiltered at 54 degrees c to total protein concentration ratios of 2:1, 3:1, 4:1, and 5:1. bifidobacterium bifidum and bifidobacterium longum were inoculated at 5% into skim milk that was not ultrafiltered (1:1) and into ultrafiltered skim milks followed by incubation at 37 degrees c. the mean maximum bacterial count (colony-forming units per milliliter) range and protein concentration ratio were from 6.25 x 10(8) to 1.69 x 10(9), skim milk; 4.42 x 10(8) ... | 1993 | 8486847 |
| the assumed assimilation of cholesterol by lactobacilli and bifidobacterium bifidum is due to their bile salt-deconjugating activity. | to study the mechanism of the propsed assimilation of cholesterol, we cultured various strains of lactobacillus acidophilus and a bifidobacterium sp. in the presence of cholesterol and oxgall. during culturing, both cholesterol and bile salts were precipitated. because of bacterial bile salt deconjugation, no conjugated bile salts were observed in either the culture fluids or the pellets. during incubation, the cell count and optical density decreased. the degree of precipitation of bile salts a ... | 1993 | 8489229 |
| biotherapeutic agents. a neglected modality for the treatment and prevention of selected intestinal and vaginal infections. | to evaluate the potential of biotherapeutic agents (microorganisms with therapeutic properties) for the prevention and/or treatment of selected intestinal and vaginal infections. | 1996 | 8596226 |
| characterization of the lactose transport system in the strain bifidobacterium bifidum dsm 20082. | lactose was fermented but not assimilated by the strain bifidobacterium bifidum dsm 20082. the sugar uptake was measured with lactose 14c. km and v(max) values were respectively 2.6 mm and 12.11 nmol/min/mg of cell protein. the lactose transport system and the beta-d-galactosidase were stimulated when the cells were grown with lactose, but isopropyl-beta-d-thiogalactopyranoside had no effect. lactose uptake was inhibited by compounds which interfered with proton and metal ionophore. na+, li+, or ... | 1996 | 8640105 |
| influence of two infant formulas and human milk on the development of the faecal flora in newborn infants. | the establishment of the faecal flora of 39 full-term infants fed exclusively on breast milk (n = 20) or with two different modern adapted cow's milk formulas (n = 19) was studied during the first 3 months of life. one formula investigated was based on 100% bovine casein as the protein source whereas the other formula contained bovine milk proteins with a whey/casein ratio of 60:40. a faecal flora rich in bifidobacteria was found in all study groups; the growth of putrefactive bacteria (especial ... | 1995 | 8645949 |
| [structural and functional aspects of oligosaccharides in human milk]. | about a century ago, pediatricians observed that in feces of breast-fed infants, compared to those of bottle-fed infants, bifidobacterium bifidum was the predominant microorganism. it was shown thereafter that aminosugar-containing oligosaccharides are growth factors for a specific strain of bifidobacterium. meanwhile, more than 130 lactose-derived oligosaccharides have been identified in human milk. some of these oligosaccharides like lacto-n-tetraose and lacto-n-fucopentaose i and ii do not oc ... | 1996 | 8815647 |
| the synthesis of galactopyranosyl derivatives with beta-galactosidases of different origins. | beta-galactosidases from four different sources were used to catalyze the transfer of beta-d-galactopyranosyl from 4-nitrophenyl-beta-d-galactopyranoside to a hydroxyl group of 2-acetamido-2-deoxy-galactopyranose in the synthesis of gal beta (1-3)galnac (1), gal beta (1-4)galnac (2) and gal beta (1-6)galnac (3), in triethyl phosphate buffered solutions (20-60%). when beta-galactosidases from penicillium multicolor and aspergillus oryzae were used as the catalysts, the beta (1-6)-linked disacchar ... | 1996 | 8870243 |
| growth characteristics of bifidobacteria in infant formulas. | four species of bifidobacteria, bifidobacterium bifidum (atcc 15696), bifidobacterium breve (atcc 15700), bifidobacterium infantis (atcc 15697), and bifidobacterium longum (atcc 15708), were grown anaerobically at 37 degrees c in lactobacilli mrs broth with 0.5% cysteine-hc1 and inoculated at 2.5% into three types of infant formula (based on soy, or milk, or casein hydrolysate) and in nonfat milk followed by incubation at 37 degrees c for 24 h. in most cases, the logarithmic phase of growth for ... | 1996 | 8872711 |
| effect of bifidogenic factors on growth characteristics of bifidobacteria in infant formulas. | a bifidogenic factor, lactulose or fructooligosaccharides, was added (0.5%) into infant formula based on soy or infant formula with hydrolyzed casein. the infant formulas were then inoculated (2.5%) with bifidobacterium bifidum (atcc 15696), bifidobacterium breve (atcc 15700), bifidobacterium infantis (atcc 15697), or bifidobacterium longum (atcc 15708) or their mixture (mixed culture) and incubated at 37 degrees c for 24 h. lactulose did not influence maximal counts or generation times in eithe ... | 1996 | 8872712 |
| the effect of cations on the hydrolysis of lactose and the transferase reactions catalysed by beta-galactosidase from six strains of lactic acid bacteria. | beta-galactosidases from lactobacillus delbruekii subsp. bulgaricus 20056, lb. casei 20094, lactococcus lactis subsp. lactis 7962, streptococcus thermophilus ts2, pediococcus pentosaceus pe39 and bifidobacterium bifidum 1901 were partially purified. the rate of hydrolysis of lactose given by the predominant beta-galactosidase activity from each of the bacteria studied was in all cases enhanced by mg2+, while the effect of k+ and na+ differed from strain to strain. the beta-galactosidases from al ... | 1996 | 8987531 |
| [the development of a nutrient medium for bifidobacterium bifidum biomass accumulation in continuous cultivation processes in a bioreactor]. | a new nutrient medium for the cultivation of bifidobacteria, containing dry nutrient bases of the bactofoc series, has been developed. the nutrient medium composition has been adjusted by modern methods with the use of a model bioscreen apparatus permitting the study of growth kinetics in 200 cultivation processes simultaneously. the new nutrient medium has been used in the development of the continuous flow cultivation of bifidobacteria in a bioreactor under anaerobic conditions. | 1996 | 9082738 |
| reduction of oxazepam to desmethyldiazepam by human intestinal bacteria. | the biotransformation of oxazepam by bifidobacterium bifidum was studied. the major metabolite was purified by chromatographic methods and found to be desmethyldiazepam using nmr, ir and other physicochemical data. | 1996 | 9132177 |
| survival of escherichia coli o157:h7 in yoghurt during preparation and storage at 4 degrees c. | cow's milk was inoculated with ca 10(3) and 10(7) cfu ml-1 escherichia coli o157:h7. after fermentation at 42 degrees c for 0-5 h, the yoghurt was stored at 4 degrees c. two kinds of yoghurt were used: traditional yoghurt (ty), made with streptococcus thermophilus and lactobacillus bulgaricus starter cultures, and 'bifido' yoghurt (by), made with the two starter cultures plus bifidobacterium bifidum. after 7 d e. coli o157:h7 decreased from 3.52 to 2.72 log10 cfu ml-1 and from 7.08 to 5.32 log10 ... | 1997 | 9172440 |
| antiproliferative effect of fermented milk on the growth of a human breast cancer cell line. | in vivo and in vitro studies have shown an antitumor activity of lactobacilli in colon cancer, and some epidemiologic studies have indicated a reduced risk of breast cancer in women who consume fermented milk products. we studied the direct effect of milk fermented by five bacteria strains (bifidobacterium infantis, bifidobacterium bifidum, bifidobacterium animalis, lactobacillus acidophilus, and lactobacillus paracasei) on the growth of the mcf7 breast cancer cell line. our results showed a gro ... | 1997 | 9200156 |
| survival of lactic acid bacteria in a dynamic model of the stomach and small intestine: validation and the effects of bile. | this study was conducted to validate a dynamic model of the stomach and small intestine to quantify the survival of lactic acid bacteria and to assess the influence of gastrointestinal secretions. the survival of a single strain of each of the following species, bifidobacterium bifidum, lactobacillus acidophilus, lactobacillus bulgaricus, and streptococcus thermophilus, was measured under physiological conditions (e.g., peristalsis, changes in ph, and changes in concentrations of enzymes and bil ... | 1997 | 9201571 |
| glucose and galactose transport in bifidobacterium bifidum dsm 20082. | sugar uptake was measured with 3h-galactose and 14c-glucose. galactose transport system was not modified by inhibitors of known translocases and did not present a saturation kinetic with high concentration of galactose. glucose incorporation was inhibited by lasalocid (cation symport inhibitor) and increased by kcl. the kinetic parameters km and vmax were respectively 9.16 mm and 26.56 nmol/min/mg cell protein. on the basis of this study, galactose crossed through the membrane by diffusion, and ... | 1997 | 9236301 |
| digoxigenin-labeled deoxyribonucleic acid probes for the enumeration of bifidobacteria in fecal samples. | the numbers of bifidobacteria in fecal samples were specifically determined by colony hybridization with the mixture of digoxigenin-labeled dna probes that were prepared from whole chromosomal dna of bifidobacterium longum 6001 and bifidobacterium adolescentis 6003. these dna probes strongly hybridized with dna of b. longum, b. adolescentis, bifidobacterium breve, bifidobacterium suis, bifidobacterium infantis, bifidobacterium bifidum, bifidobacterium angulatum, and bifidobacterium animalis. det ... | 1997 | 9241587 |
| immune modulation of blood leukocytes in humans by lactic acid bacteria: criteria for strain selection. | lactic acid bacteria in food can transiently colonize the intestine and exert beneficial effects (probiotic). survival during intestinal transit or adhesion to epithelium or both seem to be important for modifying the host's immune reactivity. because lactobacillus acidophilus strain la1 is adherent to enterocytes in vitro, we hypothesize that contact with immune cells may occur in vivo. however, bifidobacterium bifidum strain bb12, which shows high fecal colonization, is another potential immun ... | 1997 | 9250141 |
| nonlipopolysaccharide component(s) of lactobacillus acidophilus stimulate(s) the production of interleukin-1 alpha and tumor necrosis factor-alpha by murine macrophages. | previous studies in our laboratory suggested that lactobacillus acidophilus strain dds-1 (la1) has a suppressive effect on chemically induced tumors in experimental animals. in an effort to understand the possible mechanisms underlying this effect, we investigated the ability of la1 to induce the production of interleukin-1 alpha (il-1 alpha) and tumor necrosis factor-alpha (tnf-alpha), which have potent cytocidal and cytostatic effects on tumor cells. the mouse macrophage cell line raw264.7 was ... | 1997 | 9290117 |
| nmr detection of 13ch313cooh from 3-13c-glucose: a signature for bifidobacterium fermentation in the intestinal tract. | the gastrointestinal tracts of breast-fed infants are colonized more easily with bifidobacteria than are those of formula-fed infants. colonization is thought to reduce infant diarrhea. amendments to formulas that improve colonization by bifidobacteria are being actively investigated. colonization studies almost invariably require measurements of the concentration of the bifidobacteria in feces to assess their importance in the colon. we investigated the use of nuclear magnetic resonance (nmr) a ... | 1998 | 9430608 |
| [the regulation of microecological disorders of the intestines in newborn infants with perinatal pathology using the new probiotic bifidumbacterin-forte]. | the study was made on 93 newborn infants with perinatal pathology, among them infants with the perinatal lesion of the central nervous system (52), hemolytic disease of newborns (19) and conjugation jaundice (12). all newborn infants were examined for the presence of intestinal microflora in its dynamics and for the state microbiocenosis, evaluated by the rapid method based on the determination of the caseinolytic activity of fecal supernatants after the correction of normal flora with bifidumba ... | 1997 | 9460858 |
| growth, viability, and proteolytic activity of bifidobacteria in whole camel milk. | four species of bifidobacteria were evaluated for growth, viability, and proteolytic activity in whole camel milk, and comparison was made with whole cow milk. growth of all species in either whole milk was characterized by the appearance of two logarithmic phases following anaerobic incubation at 37 degrees c for 36 h. the growth rate of bifidobacterium longum 15,707 was higher in camel milk than in bovine milk and was higher for bifidobacterium angulatum 27,535 in bovine milk than in camel mil ... | 1998 | 9532489 |
| an electron microscopy study of the adherence of bifidobacterium bifidum to human intestinal caco-2 cells in vitro. | | 1998 | 9604399 |
| stimulation of cytokine production in clonal macrophage and t-cell models by streptococcus thermophilus: comparison with bifidobacterium sp. and lactobacillus bulgaricus. | the effects of four commercial strains of streptococcus thermophilus used in yogurt manufacturing on cytokine production were evaluated by using a macrophage model (raw 264.7 cells) and a t-helper-cell model (el4.il-2 thymoma cells) and compared to immunologically active strains of lactobacillus bulgaricus, bifidobacterium adolescentis, and bifidobacterium bifidum. all cytokines (tnf-alpha and il-6 in raw 264.7 cells and il-2 and il-5 in el4.il-2 cells) were affected by heat-killed s. thermophil ... | 1998 | 9678170 |
| isolation and characterization of bifidobacterium strains for probiotic formulation. | twenty-five bifidobacterium strains isolated from infant feces were identified by sugar fermentation patterns and whole-cell protein analysis. using gradient sds-page, six characteristic protein bands of the genus were detected in 40 strains of bifidobacteria but not in lactobacilli. computerized numerical analysis enabled strains to be grouped in two main clusters. strains of bifidobacterium bifidum belong to a well-differentiated cluster that joins the cluster of the remaining species at 0.582 ... | 1998 | 9678171 |
| characterization and antimicrobial spectrum of bifidocin b, a bacteriocin produced by bifidobacterium bifidum ncfb 1454. | five stains of bifidobacterium bifidum (atcc 11863 and 29591, and ncfb 1453, 1454, 1455) were examined for production of bacteriocins in mrs broth with 0.05% cysteine. only strain ncfb 1454 excreted a bacteriocin into the broth: it was designated bifidocin b. bifidocin b was sensitive to several proteolytic enzymes (protease iv, pronase e, protease xvii, proteinase k, trypsin, alpha-chymotrypsin, papain, and pepsin), but was resistant to catalase, peroxidase, lipase, lysozyme, cellulase, ribonuc ... | 1998 | 9708252 |
| beta-1,3-galactosyl-n-acetylhexosamine phosphorylase from bifidobacterium bifidum dsm 20082: characterization, partial purification and relation to mucin degradation. | a new enzyme has been characterized in a cell-free extract of bifidobacterium bifidum that catalysed the reversible phosphorolytic cleavage of beta-1,3-galacto-oligosaccharides. in the presence of pi, the phosphorolysis reaction was favoured and was accompanied by a walden reaction. cleavage of the beta-glycosidic linkage gave an alpha-galactoside derivative (alpha-d-galactose 1-phosphate). the enzyme possesses a high specificity for beta-d-galactosido-(1, 3)-n-acetylglucosamine and beta-d-galac ... | 1999 | 9889079 |
| purification, amino acid sequence and mode of action of bifidocin b produced by bifidobacterium bifidum ncfb 1454. | bifidocin b produced by bifidobacterium bifidum ncfb 1454 was purified to homogeneity by a rapid and simple three step purification procedure which included freeze drying, micro-cel adsorption/desorption and cation exchange chromatography. the purification resulted in 18% recovery and an approximately 1900-fold increase in the specific activity and purity of bifidocin b. treatment with bifidocin b caused sensitive cells to lose high amounts of intracellular k+ ions and u.v.-absorbing materials, ... | 1999 | 10030011 |
| protective effect of bifidus milk on the experimental infection with salmonella enteritidis subsp. typhimurium in conventional and gnotobiotic mice. | the ability of bifidobacterium bifidum from a commercial bifidus milk to antagonize salmonella enteritidis subsp. typhimurium in vivo, and to reduce the pathological consequences for the host, was determined using conventional and gnotobiotic mice. conventional animals received daily, by gavage, 0.1 ml bifidus milk containing about 10(9) cfu b. bifidum and germ-free animals received a single 0.1 ml dose. the conventional and gnotobiotic groups were challenged orally with 10(2) cfu of the pathoge ... | 1999 | 10063632 |
| ingestion of yogurt containing lactobacillus acidophilus and bifidobacterium to potentiate immunoglobulin a responses to cholera toxin in mice. | lactic acid bacteria have been reported to have benefits for the prevention and treatment of some forms of diarrhea and related conditions. to determine whether these effects might involve direct stimulation of the gastrointestinal immune response, we administered yogurt to try to enhance mucosal and systemic antibodies against an orally presented immunogen, cholera toxin. yogurts were manufactured with starter cultures containing different species and strains of lactic acid bacteria. mice were ... | 1999 | 10212452 |
| complementary effects of bifidogenic growth stimulators and ammonium sulfate in natural rubber serum powder on bifidobacterium bifidum. | natural rubber serum powder, rich in crude protein and carbohydrates, had a strong growth-stimulating activity for bifidobacterium bifidum jcm 1254, which was unable to grow in a fully synthetic medium, b12 assay medium. natural rubber serum powder was fractionated by ultrafiltration (molecular weight cutoff 1000). the active ultrafiltrate was further concentrated and desalted with an adsorptive microconcentrator, which adsorbs virtually all amino acids and peptides. through this purification st ... | 1999 | 10361675 |
| bifidobacterium bifidum exhibits a lipopolysaccharide-like mitogenic activity for murine b lymphocytes. | to determine the role of bifidobacteria in the systemic and mucosal antibody response, we examined the direct modulatory effect of bifidobacteria on the synthesis of antibodies by murine spleen b cells. whole spleen b cells were cultured with bifidobacterium bifidum or clostridium perfringens (welch's bacilli, negative control), and antibody synthesis was measured by elisa and enzyme-linked immunospot assay. the b. bifidum, but not c. perfringens, substantially increased total secretion of major ... | 1999 | 10509245 |
| sequence analysis of 16s rrna genes amplified from two ribosomal rna gene clusters of bifidobacterium bifidum. | two rrna gene clusters were detected in the genome of bifidobacterium bifidum kctc 3202t using southern blot analysis. to analyse the sequences of the 16s rrna genes from rrna and rrnb, 16s rdnas were amplified by pcr using dna fragments purified from gel slices containing each of the rrna gene clusters. the amplified 16s rdnas from rrna and rrnb were cloned into vectors and three clones of each gene sequenced. the resultant sequences were confirmed by direct sequencing of the 16s rdnas from rrn ... | 1999 | 10510720 |
| growth-inhibiting effects of coptis japonica root-derived isoquinoline alkaloids on human intestinal bacteria. | the growth-inhibiting activity of coptis japonica (makino) root-derived materials toward eight human intestinal bacteria was examined using an impregnated paper disk method and compared to that of four commercially available isoquinoline alkaloids [berberine sulfate (bs), berberine iodide (bi), palmatine chloride (pc), and palmatine sulfate(ps)], as well as that of thea sinensis leaf-derived epigallocatechin gallate (egcg). the biologically active constituents of the coptis extract were characte ... | 1999 | 10552394 |
| growth-inhibiting effects of cinnamomum cassia bark-derived materials on human intestinal bacteria. | the growth-inhibiting activity of cinnamomum cassia (blume) bark-derived materials toward five intestinal bacteria was examined using an impregnated paper disk method and compared with that of tetracycline and chloramphenicol, as well as four commercially available compounds (cinnamyl alcohol, trans-cinnamic acid, eugenol, and salicylaldehyde). the biologically active component of c. cassia bark was characterized as cinnamaldehyde by spectral analysis. the growth responses varied with each bacte ... | 1998 | 10554188 |
| effects of probiotic bacteria on diarrhea, lipid metabolism, and carcinogenesis: a review of papers published between 1988 and 1998. | we reviewed the evidence from human intervention studies for the health effects of probiotic bacteria, ie, live bacteria that survive passage through the gastrointestinal tract and have beneficial effects on the host. of the 49 studies reviewed, 26 dealt with the prevention or treatment of diarrheal disease, 9 with the prevention of cancer or of the formation of carcinogens, 7 with the lowering of serum cholesterol, and 7 with the stimulation of the immune system. the most widely studied probiot ... | 2000 | 10648252 |
| [the vaginal bifidobacterium flora in women of reproductive age]. | the composition of vaginal bifidoflora in 56 clinically healthy women of reproductive age was studied. the study revealed that four species of bifidobacteria, viz. bifidobacterium bifidum, b. breve, b. adolescentis 2 and b. longum, dominated in the composition of this bifidobacterial population. nine out of 11 isolated strains were found to be capable of inhibiting indicator microorganisms staphylococcus aureus and enterococcus faecalis when tested in vitro; in addition, strains b. adolescentis ... | 1999 | 10852059 |
| cordycepin: selective growth inhibitor derived from liquid culture of cordyceps militaris against clostridium spp. | the growth responses of nine human intestinal bacteria to liquid culture of cordyceps militaris link. pt. (ascomycotina: clavicipitaceae) collected from a pupa of bombyx mori l. (lepidoptera: bombycidae) were examined using spectrophotometric and impregnated paper disk methods and compared to those of tetracycline and chloramphenicol, as well as those of coptis japonica root-derived berberine chloride. the biologically active constituent of the cultures was characterized as cordycepin (3'-deoxya ... | 2000 | 10898616 |
| the efficient enzymatic synthesis of n-acetyllactosamine in an organic co-solvent. | in the presence of beta-galactosidase from bifidobacterium bifidum, n-acetyllactosamine was synthesized in significantly enhanced yield compared with earlier routes. different proportions of the (1-->4)- and (1-->6)-linked forms were obtained depending on the choice of enzyme and reaction conditions, viz. the nature of added organic co-solvent (20-80% of 2-ethoxy ethyl ether, trimethyl phosphate, or acetone). the beta-(1-->4)-linked disaccharide was the major product and the beta-(1-->6)-linked ... | 2000 | 10990022 |
| viability of probiotic (bifidobacterium, lactobacillus acidophilus and lactobacillus casei) and nonprobiotic microflora in argentinian fresco cheese. | we evaluated the suitability of argentinian fresco cheese as a food carrier of probiotic cultures. we used cultures of bifidobacterium bifidum (two strains), bifidobacterium longum (two strains), bifidobacterium sp. (one strain), lactobacillus acidophilus (two strains), and lactobacillus casei (two strains) in different combinations, as probiotic adjuncts. probiotic, lactic starter (lactococcus lactis and streptococcus thermophilus), and contaminant (coliforms, yeasts, and molds) organisms were ... | 2000 | 11003217 |
| purification and identification of a growth-stimulating peptide for bifidobacterium bifidum from natural rubber serum powder. | natural rubber serum powder, which is a by-product obtained in the production of latex rubber, has a strong growth-stimulating activity for bifidobacterium bifidum jcm 1254. the retained fraction obtained by ultrafiltration (molecular weight cutoff 1000) showed a growth-stimulating activity in a dose-dependent manner on b12 assay medium with ammonium sulfate. one of the growth stimulators was purified from the retained fraction by acetone precipitation, solid-phase extraction with a hydrophobic ... | 2000 | 11129579 |
| influence of microbial species on small intestinal myoelectric activity and transit in germ-free rats. | the effect of an intestinal microflora consisting of selected microbial species on myoelectric activity of small intestine was studied using germ-free rat models, with recording before and after specific intestinal colonization, in the unanesthetized state. intestinal transit, neuropeptides in blood (ria), and neuromessengers in the intestinal wall were determined. clostridium tabificum vp 04 promoted regular spike burst activity, shown by a reduction of the migrating myoelectric complex (mmc) p ... | 2001 | 11171619 |
| comparison of mucosal adhesion and species identification of bifidobacteria isolated from healthy and allergic infants. | fifty bifidobacteria strains were isolated from fecal samples of allergic and age matched healthy infants. allergic infants were found to have an adult type bifidobacterium flora with high levels of bifidobacterium adolescentis. healthy infants had a typical infant bifidobacterium flora with high levels of bifidobacterium bifidum. these isolates were tested for their adhesive properties to human intestinal mucus. the adhesion of the fecal bifidobacteria from healthy infants was significantly hig ... | 2001 | 11172990 |
| microbiological and biochemical properties of canestrato pugliese hard cheese supplemented with bifidobacteria. | canestrato pugliese cheeses from ewe milk were produced according to a traditional protocol and by adding 7.0 log10 cfu of fresh cells per gram of bifidobacterium bifidum bb02, bifidobacterium longum bb46, or both species. the traditional technology was modified slightly to favor the survival of probiotic microorganisms. after 56 d of ripening, the survival of b. bifidum bb02 and b. longum bb46 was 6.0 and 5.0 log10 cfu/g, respectively. after 19 d cheeses contained ca. 7.0 log10 cfu/g of bifidob ... | 2001 | 11286406 |
| intra- and extracellular beta-galactosidases from bifidobacterium bifidum and b. infantis: molecular cloning, heterologous expression, and comparative characterization. | three beta-galactosidase genes from bifidobacterium bifidum dsm20215 and one beta-galactosidase gene from bifidobacterium infantis dsm20088 were isolated and characterized. the three b. bifidum beta-galactosidases exhibited a low degree of amino acid sequence similarity to each other and to previously published beta-galactosidases classified as family 2 glycosyl hydrolases. likewise, the b. infantis beta-galactosidase was distantly related to enzymes classified as family 42 glycosyl hydrolases. ... | 2001 | 11319112 |
| enhanced antigen-specific delayed-type hypersensitivity and immunoglobulin g2b responses after oral administration of viable lactobacillus casei yit9029 in wistar and brown norway rats. | in this study, the effects of orally administered viable lactobacillus casei shirota strain yit9029 on the immunity parameters of wistar and brown norway rats were examined. for this purpose, we used the trichinella spiralis host resistance model. two weeks before and during t. spiralis infection, rats were fed 10(9) viable l. casei bacteria 5 days per week. the t. spiralis-specific delayed-type hypersensitivity (dth) response was significantly enhanced in both wistar and brown norway rats given ... | 2001 | 11427423 |
| molecular microbial analysis of bifidobacterium isolates from different environments by the species-specific amplified ribosomal dna restriction analysis (ardra). | one hundred and six isolates of the genus bifidobacterium, isolated from different environments (mainly gastrointestinal), were identified and classified taxonomically to species level by amplified ribosomal dna restriction analysis. two restriction endonucleases (sau3ai and bamhi) were chosen for aligning the 16s rrna sequences of 16 bifidobacterial species retrieved from various databases, to obtain species-specific restriction patterns. a rapid and accurate identification scheme was obtained ... | 2001 | 11451515 |
| suppressive effects of bifidobacteria on lipid peroxidation in the colonic mucosa of iron-overloaded mice. | the antioxidative effects of live bifidobacteria on lipid peroxidation in the colonic mucosa were investigated. bifidobacterium bifidum strain yakult, which has been used for production of fermented milk, most effectively inhibited lipid peroxidation catalyzed by ferrous iron in liposomes among 10 species of bifidobacteria from human intestinal flora. oral administration of b. bifidum strain yakult for 2 wk significantly decreased the level of lipid peroxide (thiobarbituric acid reactive substan ... | 2001 | 11467806 |
| the synergistic effect of lactic acid bacteria and alkylglycerols on humoral immunity in mice. | investigations on immune suppression and reconstitution of immune functions dependent on the presence of physiological microflora allow us to conclude that symbiotic microorganisms such as lactobacillus sp. are essential for adequate activity of the defense system in humans. in addition to their beneficial influence on the intestinal microbial balance, these microorganisms exert a variety of immunomodulatory effects on the host immune system. on the other hand, immunostimulatory animal-derived s ... | 2001 | 11517854 |
| [study of the physiological and biochemical characteristics of bifidobacteria at the late stages of population development]. | an investigation of the physiological and biochemical characteristics of the bifidobacterium bifidum no. 1, b. adolescentis mc-42, and b. adolescentis 94-bim strains showed that bifidobacteria with a higher growth rate produced greater amounts of the end fermentation products, acetate and lactate. the growth of the strains in batch cultures was found to be inhibited by acidic fermentation products. the growth of b. bifidum no. 1 in a batch mode lasted 100 h at a population density of 10(6) cfu/m ... | 2001 | 11558275 |
| preventive effects of bifidobacterium- and lactobacillus-fermented milk on the development of inflammatory bowel disease in senescence-accelerated mouse p1/yit strain mice. | we recently discovered inflammatory bowel disease (ibd) in the ileum and cecum of samp1/yit strain mice under specific pathogen-free (spf) conditions. to determine the effect of lactic acid bacteria (lab - bifidobacterium breve, bifidobacterium bifidum and lactobacillus acidophilus)-fermented milk on the prevention of ibd in samp1/yit strain mice, we compared the disease severity on the intestinal inflammation among the group of mice fed saline, unfermented milk or lab-fermented milk. | 2001 | 11684822 |
| growth and viability of commercial bifidobacterium spp. in honey-sweetened skim milk. | two commercial bifidobacterium bifidum (bf-1 and bf-6) were cultured in 12% (wt/vol) reconstituted nonfat dry milk (ndm) containing 5% (wt/wt) honey, sucrose, fructose, or glucose. inoculated samples were incubated anaerobically at 37 degrees c for 48 h. samples were collected at 12-h intervals and examined for (i) specific growth rate, (ii) ph, and (iii) levels of fermentation end products (lactic and acetic acids) as measured by high-performance liquid chromatography (hplc). viability of the o ... | 2001 | 11726158 |
| the use of probiotics in gastrointestinal disease. | probiotics are living microorganisms that can affect the host in a beneficial manner. prebiotics are nondigestible food ingredients that stimulate the growth and activity of probiotic bacteria already established in the colon. efficacy of probiotic compounds has been shown in a wide range of gastrointestinal diseases. lactobacillus gg alone, or the combination of bifidobacterium bifidum and streptococcus thermophilus, is effective in the treatment of clostridium difficile, as well as in preventi ... | 2001 | 11773948 |
| high-efficiency synthesis of oligosaccharides with a truncated beta-galactosidase from bifidobacterium bifidum. | an exceptionally large beta-galactosidase, bif3, with a subunit molecular mass of 188 kda (1,752 amino acid residues) was recently isolated from bifidobacterium bifidum dsm20215 [møller et al. (2001) appl environ microbiol 67:2276-2283]. the bif3 polypeptide comprises a signal peptide followed by an n-terminal beta-galactosidase region and a c-terminal galactose-binding motif. we have investigated the functional importance of the c-terminal part of the bif3 sequence by deletion mutagenesis and e ... | 2001 | 11778873 |
| oral supplementation with lactic acid-producing bacteria during intake of clindamycin. | objective: to study the effect of administration of clindamycin with or without supplementation of the intestinal microflora with bifidobacterium bifidum and lactobacillus acidophilus. methods: twenty-three healthy subjects received clindamycin by mouth for 7 days. eleven of the subjects also received capsules containing lyophilized l. acidophilus and b. bifidum for 14 days. the other 12 subjects received placebo. results: there was a marked decrease in total numbers of anaerobic bacteria during ... | 1997 | 11864087 |
| bifidobacterium bifidum monoassociation of gnotobiotic mice: effect on enterocyte brush-border enzymes. | the effect of intestinal colonization with bifidobacterium bifidum (gram-positive anaerobic bacterium colonizing the intestine of healthy new-born mammals, exhibiting a probiotic effect, protecting the intestinal mucosa against colonization by pathogenic microflora) on enterocyte brush-border enzymes was examined in weaned 23-d- and in 2-month-old gnotobiotic inbred mice and compared with that in corresponding germ-free (gf) and conventional (cv) controls. the two groups of gf mice were associat ... | 2001 | 11898351 |
| selective responses of three ginkgo biloba leaf-derived constituents on human intestinal bacteria. | the selective responses of ginkgo biloba leaf-derived materials against six intestinal bacteria was examined using an impregnated paper disk method and compared with that of bilobalide, ginkgolides a and b, kaempferol, and quercetin. the components of g. biloba leaves were characterized as kaempferol 3-o-alpha-(6' "-p-coumaroylglucosyl-beta-1,4-rhamnoside), kaempferol 3-o-(2' '-o-beta-d-glucopyranosyl)-alpha-l-rhamnopyranoside, and quercetin 3-o-alpha-(6' "-p-coumaroylglucosyl-beta-1,4-rhamnosid ... | 2002 | 11902921 |
| lactoferrin-binding proteins in bifidobacterium bifidum. | lactoferrin is an iron-binding glycoprotein and its bacteriostatic and bactericidal effects on gram-positive and gram-negative bacteria are well known. on the other hand, it is known that certain kinds of lactic acid bacteria are resistant to its antibacterial effects. moreover, it is reported that lactoferrin promotes the growth of bifidobacteria in in vitro and in vivo experiments. in our experiments, lactoferrin-binding protein was found both in the membrane and cytosolic fractions of bifidob ... | 2002 | 11908648 |
| evolution of carbohydrate fraction in carbonated fermented milks as affected by beta-galactosidase activity of starter strains. | the influence of carbonation on the evolution of lactose, galactose and glucose in fermented milks with added probiotic bacteria (lactobacillus casei, lactobacillus acidophilus and/or bifidobacterium bifidum) was evaluated and related to beta-galactosidase activity of starter strains. during incubation and first days of refrigeration, lactose hydrolysis resulting in the liberation of galactose and glucose occurred in ct (streptococcus thermophilus/lb. casei), at (str. thermophilus/lb. acidophilu ... | 2002 | 12047103 |
| detection of aminotransferase activity of propionibacterium freudenreichii after sds-page. | aminotransferases (ats) had previously been detected after native electrophoresis. we show now that aminotransferase(s) of propionibacterium freudenreichii can be detected after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). moreover, it retained a high activity (84%) in the presence of 0.23% sds, contrary to what was observed for aminotransferase(s) of bifidobacterium bifidum (54%) and of six other cheese-related species (0-20%). | 2002 | 12069890 |
| production of oligosaccharides in yogurt containing bifidobacteria and yogurt cultures. | yogurts were prepared by using yogurt cultures combined to mixed cultures of bifidobacteria (bifidobacterium animalis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, and bifidobacterium longum) and by adding a preincubation step (1.5 h at 50 degrees c) with bifidobacteria to the conventional method of manufacture in order to produce oligosaccharides. the survival of bifidobacteria was drastically affected during storage of yogurts, except for products containing b. ani ... | 2002 | 12086039 |
| a pcr-based method for identification of lactobacilli at the genus level. | we developed a polymerase chain reaction (pcr)-based method for the identification of lactobacilli at the genus level. one specific primer, lblma1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16s and 23s rrna genes in a number of lactobacillus strains. amplification with lblma1-rev and r16-1, a universal primer, generated a pcr product for 23 lactobacillus species. electrophoresis did not reveal any discrete bands when escherichia coli, la ... | 2002 | 12351242 |
| selection of human isolates of bifidobacteria for their use as probiotics. | an attempt was made to isolate human strains of bifidobacteria, all together 36, from fecal samples of 15 breast-fed infants ages 1-6 mo. these isolates were checked for their ability to grow in the presence of 1-3% bile, 0.2-0.4% phenol, and low ph (3.0-5.0) in vitro, to evaluate their capacity to grow under hostile and unfavorable conditions of the human digestive tract. because milk is to be used as a carrier medium, their ability to grow in 10% sterile skim milk was also evaluated. the bifid ... | 2002 | 12396113 |
| encapsulated bifidobacterium bifidum potentiates intestinal iga production. | we asked whether bifidobacterium bifidum regulates the synthesis of iga by mucosal lymphoid cells. b. bifidum alone, but not clostridium perfringens, significantly induced total iga and igm synthesis by both mesenteric lymph nodes (mln) and peyer's patch (pp) cells. we, further, investigated the mucosal antibody production following peroral administration of b. bifidum to mice. ingested b. bifidum significantly increased the number of ig (igm, igg, and iga) secreting cells in the culture of both ... | 2002 | 12473264 |
| the effects of bifidobacterium bifidum ofr9, a strain resistant to antituberculosis and antileprosy agents, on fecal flora in mice. | since bifidobacterium bifidum, one of the strains of medical preparations used for human intestinal disorders, is sensitive to rifampicin (rfp) and fluoroquinolones, its therapeutic effect cannot be guaranteed when it is administered concomitantly with these antibiotics. to develop new strains of b. bifidum that are resistant to these drugs, b. bifidum rfr61, which is highly resistant to rfp, was selected by the n-methyl-n'-nitrosoguanidine (mnng) mutation method. then, b. bifidum ofr9 was selec ... | 1997 | 12501354 |