| photochemical treatment of platelet concentrates with amotosalen and long-wavelength ultraviolet light inactivates a broad spectrum of pathogenic bacteria. | bacterial contamination of platelet (plt) concentrates can result in transfusion-transmitted sepsis. a photochemical treatment (pct) process with amotosalen hcl and long-wavelength ultraviolet light (uva), which cross-links nucleic acids, was developed to inactivate bacteria and other pathogens in plt concentrates. | 2004 | 15383024 |
| lactate-utilizing bacteria, isolated from human feces, that produce butyrate as a major fermentation product. | the microbial community of the human colon contains many bacteria that produce lactic acid, but lactate is normally detected only at low concentrations (<5 mm) in feces from healthy individuals. it is not clear, however, which bacteria are mainly responsible for lactate utilization in the human colon. here, bacteria able to utilize lactate and produce butyrate were identified among isolates obtained from 10(-8) dilutions of fecal samples from five different subjects. out of nine such strains ide ... | 2004 | 15466518 |
| comparison of compositions and metabolic activities of fecal microbiotas in young adults and in antibiotic-treated and non-antibiotic-treated elderly subjects. | the colonic microbiota mediates many cellular and molecular events in the host that are important to health. these processes can be affected in the elderly, because in some individuals, the composition and metabolic activities of the microbiota change with age. detailed characterizations of the major groups of fecal bacteria in healthy young adults, in healthy elderly people, and in hospitalized elderly patients receiving antibiotics were made in this study, together with measurements of their m ... | 2004 | 15466557 |
| beta-galactosidase from bifidobacterium adolescentis dsm20083 prefers beta(1,4)-galactosides over lactose. | abstract a beta-galactosidase gene (beta-gal ii) from bifidobacterium adolescentis dsm 20083 was cloned into a pbluescript sk (-) vector and expressed in escherichia coli. the recombinant enzyme was purified from the cell extract by anion-exchange and size-exclusion chromatography. beta-gal ii had a native molecular mass of 235 kda and the subunits had a molecular mass of 81 kda, indicating that beta-gal ii occurs as a trimer. the enzyme was classified as belonging to glycosyl hydrolase family 4 ... | 2004 | 15480628 |
| variation of microbiological and biochemical parameters in the faeces of two healthy people over a 15 day period. | recent years have seen increasing interest in the complex microbial ecosystem of the human gastrointestinal tract. knowledge of its microbial colonists and their beneficial/detrimental activities is important. however, generalized assumptions about the microbial composition of the human gut should be taken with caution until more studies in different human communities have been conducted. the capacity of modulating or inhibiting harmful populations through high doses of beneficial microorganisms ... | 2004 | 15597174 |
| cloning and characterization of arabinoxylan arabinofuranohydrolase-d3 (axhd3) from bifidobacterium adolescentis dsm20083. | arabinoxylan arabinofuranohydrolase-d3 (axhd3) from bifidobacterium adolescentis releases only c3-linked arabinose residues from double-substituted xylose residues. a genomic library of b. adolescentis dsm20083 was screened for the presence of the axhd3 gene. two plasmids were identified containing part of the axhd3 gene. the nucleotide sequences were combined and three open reading frames (orfs) were found. the first orf showed high homology with xylanases belonging to family 8 of the glycoside ... | 2005 | 15650848 |
| selective growth-inhibiting effects of compounds identified in tabebuia impetiginosa inner bark on human intestinal bacteria. | the growth-inhibiting activity of anthraquinone-2-carboxylic acid and lapachol identified in the inner bark of taheebo, tabebuia impetiginosa, toward 10 human intestinal bacteria was evaluated by using a paper disk diffusion bioassay and compared to those of seven lapachol congeners (1,4-naphthoquinone, naphthazarin, menadione, lawsone, plumbagin, juglone, and dichlone) as well as two commercially available antibiotics, chloramphenicol and tetracycline. anthraquinone-2-carboxylic acid exhibited ... | 2005 | 15713033 |
| bacteria recovered from dental pulp induce apoptosis of lymph node cells. | apoptosis is critical in the pathogenesis of several infectious diseases. the induction of apoptosis was assessed in mouse lymph node cells by four bacteria recovered from infected human dental pulp: gemella morbillorum, clostridium butyricum, fusobacterium nucleatum and bifidobacterium adolescentis. smaller lymph nodes and smaller numbers of cells were observed after experimental dental pulp infection with c. butyricum, suggesting that this bacterium induces cell death. apoptosis was evaluated ... | 2005 | 15770029 |
| quantitative real-time pcr assays to identify and quantify fecal bifidobacterium species in infants receiving a prebiotic infant formula. | a healthy intestinal microbiota is considered to be important for priming of the infants' mucosal and systemic immunity. breast-fed infants typically have an intestinal microbiota dominated by different bifidobacterium species. it has been described that allergic infants have different levels of specific bifidobacterium species than healthy infants. for the accurate quantification of bifidobacterium adolescentis, bifidobacterium angulatum, bifidobacterium bifidum, bifidobacterium breve, bifidoba ... | 2005 | 15870317 |
| type i arabinogalactan contains beta-d-galp-(1-->3)-beta-d-galp structural elements. | arabinogalactan type i from potato was partially degraded by endo-galactanase from aspergillus niger. high-performance anion-exchange chromatography revealed that several of the oligomeric degradation products eluted as double peaks. to investigate the nature of these products, the digest was fractionated by bio-gel p2 chromatography. the pool that contained tetramers was treated with a beta-d-galp-(1-->4)-specific galactosidase from bifidobacterium adolescentis to obtain a dimer with deviating ... | 2005 | 16054605 |
| genetic diversity of viable, injured, and dead fecal bacteria assessed by fluorescence-activated cell sorting and 16s rrna gene analysis. | a novel approach combining a flow cytometric in situ viability assay with 16s rrna gene analysis was used to study the relationship between diversity and activity of the fecal microbiota. simultaneous staining with propidium iodide (pi) and syto bc provided clear discrimination between intact cells (49%), injured or damaged cells (19%), and dead cells (32%). the three subpopulations were sorted and characterized by denaturing gradient gel electrophoresis (dgge) of 16s rrna gene amplicons obtaine ... | 2005 | 16085863 |
| effect of ph on an in vitro model of gastric microbiota in enteral nutrition patients. | patients with dysphagia due to oropharyngeal disease or cerebrovascular accident require long-term nutritional support via enteral feeding, which often results in microbial overgrowth in the upper gastrointestinal (gi) tract. gastric acid is the primary innate defense mechanism in the stomach and has been assumed to provide an effective barrier to microbial colonization at ph values of <4. to evaluate the efficacy of gastric acid as a barrier to overgrowth, the microbiota of gastric and duodenal ... | 2005 | 16085875 |
| cloning and characterization of a bile salt hydrolase (bsh) from bifidobacterium adolescentis. | a gene coding for bile salt hydrolase (bsh) from bifidobacterium adolescentis was cloned and expressed in escherichia coli, and the nucleotide sequence was determined. the bsh of e. coli transformants was produced intracellularly in the absence of bile salts. a unique bsh promoter (p(bsh)) sequence was identified by using a neural network promoter prediction (nnpp, version 2.2). in spite of their high-level sequence homology with other bsh genes in the bifidobacterium species, their genetic orga ... | 2005 | 16086241 |
| a novel procedure for the isolation of glycolipids from bifidobacterium adolescentis 94 bim using supercritical carbon dioxide. | this study describes a novel isolation procedure for major glycolipids from bifidobacterium adolescentis 94 bim. the procedure consists of the use of supercritical carbon dioxide (scco(2)) with hydro-methanolic solution as co-solvent. the major glycolipids were isolated using the following operating conditions: pressure, 30 mpa, co-solvent concentration, 10% (9:1, methanol/water, v/v), co(2) flow rate, 5 g/min, extraction time and temperature, 2h and 55 degrees c, respectively. the reference gly ... | 2006 | 16229918 |
| conditions affecting cell surface properties of human intestinal bifidobacteria. | the cell surface properties of human intestinal bifidobacteria have been characterized for 30 strains isolated from a fecal sample. strain identification to the species level was obtained by restriction analysis of the amplified 16s rrna gene and confirmed by dna/dna reassociation experiments. the isolates were grouped in four genetically homogeneous clusters whose members belonged to bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium longum and bifidobacterium pseudocatenula ... | 2005 | 16284927 |
| increasing the transglycosylation activity of alpha-galactosidase from bifidobacterium adolescentis dsm 20083 by site-directed mutagenesis. | the alpha-galactosidase (aga) from bifidobacterium adolescentis dsm 20083 has a high transglycosylation activity. the optimal conditions for this activity are ph 8, and 37 degrees c. at high melibiose concentration (600 mm), approximately 64% of the enzyme-substrate encounters resulted in transglycosylation. examination of the acceptor specificity showed that aga required a hydroxyl group at c-6 for transglycosylation. pentoses, hexuronic acids, deoxyhexoses, and alditols did not serve as accept ... | 2006 | 16320365 |
| characterization of human intestinal bifidobacteria using competitive pcr and pcr-ttge. | in this study, a competitive pcr was developed to estimate the quantity of bifidobacteria in human faecal samples using two 16s rrna gene bifidobacterium genus-specific primers, bif164f and bif662r. a pcr-temporal temperature gradient gel electrophoresis (ttge) with the same primers also allowed us to describe the bifidobacterium species present in these faecal samples. the pcr product obtained from the competitor had 467 bp, and was 47 bp shorter than the pcr products obtained from bifidobacter ... | 2006 | 16420612 |
| determination of survival, identity and stress resistance of probiotic bifidobacteria in bio-yoghurts. | to determine the level of bifidobacteria in bio-yoghurts in the uk, identify the species, and compare the resistance of common bifidobacterium spp. to acidity and oxidative stress. | 2006 | 16478503 |
| expression and secretion of bifidobacterium adolescentis amylase by bifidobacterium longum. | bifidobacterium adolescentis int-57 (int57), isolated from human feces, secretes an amylase. we have shot-gun cloned, sequence analyzed and expressed the gene encoding this amylase in b. longum. the sequenced 2477 bp fragment was homologous to other extracellular amylases. the encoded protein was predicted to be composed of 595 amino acids with a molecular weight of 64 kda, and was designated amyb. highly conserved amylase domains were found in amyb. the signal sequence and cleavage site was pre ... | 2006 | 16489493 |
| augmentation of vaccenate production and suppression of vaccenate biohydrogenation in cultures of mixed ruminal microbes. | to increase ruminal outflow of trans-vaccenic acid (t-va), a new strain of butyrivibrio fibrisolvens (mdt-10) was isolated that has a great ability to hydrogenate linoleic acid (la) to t-va. when strain mdt-10 was added to the batch cultures of mixed ruminal microbes (1% of the total number of viable ruminal bacteria), la conversion to t-va increased greatly; after 3 h, t-va levels were > 4-fold higher than the control. by 10 h, all of the t-va was hydrogenated to stearic acid. however, when a n ... | 2006 | 16507700 |
| method for direct selection of potentially probiotic bifidobacterium strains from human feces based on their acid-adaptation ability. | a method for direct selection of acid-resistant bifidobacterium strains was developed by prolonged exposure of human feces to homologous lethal stress conditions. the recovered strains were intrinsically resistant to acidic gastric conditions (ph 2.0) and also showed good tolerance to high concentrations of bile salts and nacl. bifidobacterium breve and bifidobacterium adolescentis were, respectively, the infant- and adult-type bifidobacterial species showing the highest ability to develop an ac ... | 2006 | 16522337 |
| [anaerobic bacteria isolated from patients with suspected anaerobic infections]. | the study involved 394 clinical samples sent to the clinical microbiology laboratory of hacettepe university adult hospital between january 1997 and may 2004 for anaerobic cultivation. since multiple cultures from the same clinical samples of the same patient were excluded, the study was carried on 367 samples. the anaerobic cultures were performed in anaerobic jar using anaerogen kits (oxoid, basingstoke, u.k.) or genbox (biomérieux, lyon, france). the isolates were identified by both classical ... | 2005 | 16544546 |
| two routes of metabolic cross-feeding between bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut. | dietary carbohydrates have the potential to influence diverse functional groups of bacteria within the human large intestine. of 12 bifidobacterium strains of human gut origin from seven species tested, four grew in pure culture on starch and nine on fructo-oligosaccharides. the potential for metabolic cross-feeding between bifidobacterium adolescentis and lactate-utilizing, butyrate-producing firmicute bacteria related to eubacterium hallii and anaerostipes caccae was investigated in vitro. e. ... | 2006 | 16672507 |
| substrate preference of bifidobacterium adolescentis mb 239: compared growth on single and mixed carbohydrates. | the utilization of mono-, di-, and oligosaccharides by bifidobacterium adolescentis mb 239 was investigated. raffinose, fructooligosaccharides (fos), lactose, and the monomeric moieties glucose and fructose were used. to establish a hierarchy of sugars preference, the kinetics of growth and sugar consumption were determined on individual and mixed carbohydrates. on single carbon sources, higher specific growth rates and cell yields were attained on di- and oligosaccharides compared to monosaccha ... | 2006 | 16865345 |
| [patterns of growth and beta-galactosidase production by bifidobacteria]. | we investigated the patterns of growth and beta-galactosidase formation in the strains bifidobacterium adolescentis go-13, ms-42, 91-bim, and 94-bim, and b. bifidum no. 1, lva-3, 791 on media with various carbon sources. the synthesis of beta-galactosidase was shown to be associated with exponential growth of the cultures involved. the maximum specific rate of beta-galactosidase synthesis of 0.20 u mg(-1) h(-1) was observed in b. bifidum lva-3 after 3-6 h of cultivation. this value for b. adoles ... | 2006 | 16871798 |
| rapid detection of human fecal contamination in estuarine environments by pcr targeting of bifidobacterium adolescentis. | detection of bifidobacterium adolescentis was used as an effective genetic marker of human fecal contamination in georgia estuaries. enterococci enumerations on mei media indicated that a tributary to the little satilla river with 516 cfu/100 ml was the most polluted of all the rivers tested. extracted dna from eight river water samples was subjected to a two-step nested pcr protocol using genus and species specific primers for bifidobacterium spp. and b. adolescentis. b. adolescentis was detect ... | 2007 | 16876892 |
| co-culture of bifidobacterium adolescentis and bacteroides thetaiotaomicron in arabinogalactan-limited chemostats: effects of dilution rate and ph. | the effects of dilution rate (d = 0.04-0.38/h) and ph (5.0-6.5) on co-cultures of bifidobacterium adolescentis and bacteroides thetaiotaomicron were studied in arabinogalactan-limited chemostats. b. thetaiotaomicron outcompeted b. adolescentis at all dilution rates at culture ph values between 5.0 and 6.0, although the bifidobacterium was always detected in the fermenters. at ph 6.5, however, b. adolescentis predominated in co-cultures at dilution rates above 0.24/h. arabinogalactan degrading en ... | 1995 | 16887536 |
| mutagenicity of arbutin in mammalian cells after activation by human intestinal bacteria. | arbutin (hydroquinone-beta-d-glucopyranoside) is present in various food plants. its aglycone, hydroquinone, is mutagenic and carcinogenic. we investigated whether hydroquinone may be released under conditions encountered in the human gastrointestinal tract. arbutin was stable in artificial gastric juice. fecal slurries from nine human subjects completely converted arbutin (2 mm) into hydroquinone. four of nine representative human intestinal species investigated, namely eubacterium ramulus, ent ... | 2006 | 16904805 |
| integrated analysis of established and novel microbial and chemical methods for microbial source tracking. | several microbes and chemicals have been considered as potential tracers to identify fecal sources in the environment. however, to date, no one approach has been shown to accurately identify the origins of fecal pollution in aquatic environments. in this multilaboratory study, different microbial and chemical indicators were analyzed in order to distinguish human fecal sources from nonhuman fecal sources using wastewaters and slurries from diverse geographical areas within europe. twenty-six par ... | 2006 | 16957211 |
| molecular monitoring of the fecal microbiota of healthy human subjects during administration of lactulose and saccharomyces boulardii. | diet is a major factor in maintaining a healthy human gastrointestinal tract, and this has triggered the development of functional foods containing a probiotic and/or prebiotic component intended to improve the host's health via modulation of the intestinal microbiota. in this study, a long-term placebo-controlled crossover feeding study in which each subject received several treatments was performed to monitor the effect of a prebiotic substrate (i.e., lactulose), a probiotic organism (i.e., sa ... | 2006 | 16957220 |
| structural rearrangements of sucrose phosphorylase from bifidobacterium adolescentis during sucrose conversion. | the reaction mechanism of sucrose phosphorylase from bifidobacterium adolescentis (bisp) was studied by site-directed mutagenesis and x-ray crystallography. an inactive mutant of bisp (e232q) was co-crystallized with sucrose. the structure revealed a substrate-binding mode comparable with that seen in other related sucrose-acting enzymes. wild-type bisp was also crystallized in the presence of sucrose. in the dimeric structure, a covalent glucosyl intermediate was formed in one molecule of the b ... | 2006 | 16990265 |
| [effect of oral lactosucrose supplementation on human enteric oxalate-degrading bacteria]. | a variety of oxalate-degrading bacteria including oxalobacter formigenes and some species of bifidobacterium are known to colonize the human intestinal tract. oral lactosucrose supplementation promotes the growth of bifidobacterium in the human intestine. therefore, we investigated the effect of oral lactosucrose supplementation on enteric oxalate-degrading bacteria in twelve healthy men (age ranging from 25 to 39 years). lactosucrose was orally administered 10 g daily for 2 weeks without restri ... | 2006 | 17040052 |
| population dynamics of bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry. | the population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (fish) coupled with flow cytometry (fcm) analysis. although double-staining fish-fcm using both fluorescein isothiocyanate (fitc) and indodicarbocyanine (cy5) as labeling dyes for fecal samples has been reported, the analysis was interfered with by strong autofluorescence at the fitc fluorescence region because of the presence ... | 2006 | 17056700 |
| selective colonization of insoluble substrates by human faecal bacteria. | insoluble plant polysaccharides and endogenous mucin are important energy sources for human colonic microorganisms. the object of this study was to determine whether or not specific communities colonize these substrates. using faecal samples from four individuals as inocula for an anaerobic in vitro continuous flow system, the colonization of wheat bran, high amylose starch and porcine gastric mucin was examined. recovered substrates were extensively washed and the remaining tightly attached bac ... | 2007 | 17298367 |
| quantitative assessment of faecal bifidobacterial populations by real-time pcr using lanthanide probes. | to develop real-time quantitative pcr methods, based on the use of probes labelled with a stable fluorescent lanthanide chelate, for the quantification of different human faecal bifidobacterial populations. | 2007 | 17381755 |
| kinetics and metabolism of bifidobacterium adolescentis mb 239 growing on glucose, galactose, lactose, and galactooligosaccharides. | the kinetics and the metabolism of bifidobacterium adolescentis mb 239 growing on galactooligosaccharides (gos), lactose, galactose, and glucose were investigated. an unstructured unsegregated model for growth in batch cultures was developed, and kinetic parameters were calculated with a recursive algorithm. the growth rate and cellular yield were highest on galactose, followed by lactose and gos, and were lowest on glucose. lactate, acetate, and ethanol yields allowed the calculation of carbon ... | 2007 | 17434997 |
| identification of commensal bacterial strains that modulate yersinia enterocolitica and dextran sodium sulfate-induced inflammatory responses: implications for the development of probiotics. | an increasing body of evidence suggests that probiotic bacteria are effective in the treatment of enteric infections, although the molecular basis of this activity remains elusive. to identify putative probiotics, we tested commensal bacteria in terms of their toxicity, invasiveness, inhibition of yersinia-induced inflammation in vitro and in vivo, and modulation of dextran sodium sulfate (dss)-induced colitis in mice. the commensal bacteria escherichia coli, bifidobacterium adolescentis, bacter ... | 2007 | 17485456 |
| recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from bifidobacterium adolescentis. | the family 8 glycoside hydrolase (rexa) from bifidobacterium adolescentis was expressed in escherichia coli. the recombinant enzyme was characterized as a reducing-end xylose-releasing exo-oligoxylanase. apart from giving insights into this new class of enzymes, knowledge of the rexa enzyme helps to postulate a mechanism for the b. adolescentis breakdown of prebiotic xylooligosaccharides. | 2007 | 17586675 |
| molecularly assessed shifts of bifidobacterium ssp. and less diverse microbial communities are characteristic of 5-year-old allergic children. | the composition of intestinal microbiota and the bifidobacterium group community in 20 allergic and 20 nonallergic 5-year-old children was visualized by pcr-denaturing gradient gel electrophoresis (dgge). the number of dominant bands in the dgge profiles was smaller in allergic children than in nonallergic children (p<0.001). allergic children mainly formed a single group upon cluster analysis, whereas nonallergic children were divided between four different groups. in allergic children the bifi ... | 2007 | 17868362 |
| differences in faecal bacterial communities in coeliac and healthy children as detected by pcr and denaturing gradient gel electrophoresis. | coeliac disease (cd) is a chronic inflammatory disorder of the small intestinal mucosa. scientific evidence supports a role of the gut microbiota in chronic inflammatory disorders; yet information is not specifically available for cd. in this study, a comparative denaturing gradient gel electrophoresis analysis of faecal samples from coeliac children and age-matched controls was carried out. the diversity of the faecal microbiota was significantly higher in coeliac children than in healthy contr ... | 2007 | 17919298 |
| maternal breast-milk and intestinal bifidobacteria guide the compositional development of the bifidobacterium microbiota in infants at risk of allergic disease. | the sources and the impact of maternal bacteria on the initial inoculum of the intestinal microflora of newborn infants remain elusive. | 2007 | 17941914 |
| reduction of vitamin k concentration by salivary bifidobacterium strains and their possible nutritional competition with porphyromonas gingivalis. | to assess the possibility that bifidobacteria compete with porphyromonas gingivalis for their mutual growth factor vitamin k. this study also examined whether salivary bifidobacterium species decrease vitamin k concentration in the growth medium. | 2007 | 17953607 |
| in vitro fermentability of human milk oligosaccharides by several strains of bifidobacteria. | this study was conducted to investigate the catabolism and fermentation of human milk oligosaccharides (hmo) by individual strains of bifidobacteria. oligosaccharides were isolated from a pooled sample of human milk using solid-phase extraction, and then added to a growth medium as the sole source of fermentable carbohydrate. of five strains of bifidobacteria tested (bifidobacterium longum biovar infantis, bifidobacterium bifidum, bifidobacterium longum biovar longum, bifidobacterium breve, and ... | 2007 | 17966141 |
| influence of alternansucrase-derived oligosaccharides and other carbohydrates on alpha-galactosidase and alpha-glucosidase activity in bifidobacterium adolescentis. | to determine the influence of alternansucrase-derived oligosaccharides (aos) and other carbohydrates on alpha-galactosidase and alpha-glucosidase activity in bifidobacterium adolescentis. | 2008 | 17971098 |
| bifidobacteria in feces and environmental waters. | bifidobacteria have been recommended as potential indicators of human fecal pollution in surface waters even though very little is known about their presence in nonhuman fecal sources. the objective of this research was to shed light on the occurrence and molecular diversity of this fecal indicator group in different animals and environmental waters. genus- and species-specific 16s rrna gene pcr assays were used to study the presence of bifidobacteria among 269 fecal dna extracts from 32 differe ... | 2008 | 17993557 |
| bifidobacterium carbohydrases-their role in breakdown and synthesis of (potential) prebiotics. | there is an increasing interest to positively influence the human intestinal microbiota through the diet by the use of prebiotics and/or probiotics. it is anticipated that this will balance the microbial composition in the gastrointestinal tract in favor of health promoting genera such as bifidobacterium and lactobacillus. carbohydrates like non-digestible oligosaccharides are potential prebiotics. to understand how these bacteria can grow on these carbon sources, knowledge of the carbohydrate-m ... | 2008 | 18040988 |
| interactions between salivary bifidobacterium adolescentis and other oral bacteria: in vitro coaggregation and coadhesion assays. | coaggregation assays were performed to investigate interactions between oral bifidobacterium adolescentis and other oral bacterial species. bifidobacterium adolescentis olb6410 isolated from the saliva of healthy humans did not coaggregate with actinomyces naeslundii jcm8350, streptococcus mitis ols3293, streptococcus sanguinis jcm5708, veillonella parvula atcc17745 or porphyromonas gingivalis ob7124, but it did coaggregate with fusobacterium nucleatum jcm8532. subsequent examination of biofilm ... | 2008 | 18312575 |
| bifidobacterium microbiota and parameters of immune function in elderly subjects. | faecal and serum samples were collected over a period of 6 months from 55 institutionalized elderly subjects, who were enrolled in a double-blind placebo-controlled study. participants were randomized in one of the three treatment groups: intervention (two probiotic bifidobacterium longum strains: 2c and 46), placebo and commercial control (bifidobacterium lactis bb-12). the faecal bifidobacterium microbiota was characterized by genus and species-specific pcr. serum levels of the cytokines il-10 ... | 2008 | 18336547 |
| inhibition of proliferation in colon cancer cell lines and harmful enzyme activity of colon bacteria by bifidobacterium adolescentis spm0212. | in this study, we assessed the anticancer activity and bacterial enzyme inhibition of bifidobacterium adolescentis spm0212. b. adolescentis spm0212 inhibited the proliferation of three human colon cancer cell lines: ht-29, sw 480, and caco-2. spm0212 also dose-dependently inhibited tnf-á production and changes in cellular morphology. b. adolescentis spm0212 inhibited harmful fecal enzymes, including â-glucuronidase, â-glucosidase, tryptophanase, and urease. thus, b. adolescentis spm0212 exerts a ... | 2008 | 18449504 |
| detection of blastocystis from stool samples using real-time pcr. | we developed a real-time lc pcr assay to detect a 152 bp sequence in an uncharacterized region of the blastocystis genome. the described assay detected 11 of 11 atcc strains of blastocystis from subtypes 1, 3, and 4. three of three stool samples from oregon and california military personnel that were negative for blastocystis by an ova and parasite test as well as a conventional pcr assay were positive for blastocystis using our real-time lc pcr assay. diagnosis of blastocystis infections using ... | 2008 | 18488250 |
| coaggregation between and among human intestinal and oral bacteria. | coaggregation is believed to facilitate the integration of new bacterial species into polymicrobial communities. the aim of this study was to investigate coaggregation between and among human oral and enteric bacteria. stationary phase cultures of 10 oral and 10 enteric species, chosen on the basis of numerical and ecological significance in their respective environments together with their ease of cultivation, were tested using a quantitative spectrophotometric coaggregation assay in all possib ... | 2008 | 18554305 |
| use of specific primers based on the 16s-23s internal transcribed spacer (its) region for the screening bifidobacterium adolescentis in yogurt products and human stool samples. | effective methods for the identification and enumeration of lactic acid producing bacteria (lab) cells are important for the quality control and assurance of probiotic products. in this study, we designed a polymerase chain reaction (pcr) primer set from the sequence in 16s-23s internal transcribed spacer (its) region and used it for the specific detection of bifidobacterium adolescentis, one of the bifidobacterium species used in probiotics. specificity of the pcr primers, i.e., bits-1/bits-2, ... | 2008 | 18565771 |
| effect of inulin on the human gut microbiota: stimulation of bifidobacterium adolescentis and faecalibacterium prausnitzii. | prebiotics are food ingredients that improve health by modulating the colonic microbiota. the bifidogenic effect of the prebiotic inulin is well established; however, it remains unclear which species of bifidobacterium are stimulated in vivo and whether bacterial groups other than lactic acid bacteria are affected by inulin consumption. changes in the faecal microbiota composition were examined by real-time pcr in twelve human volunteers after ingestion of inulin (10 g/d) for a 16-d period in co ... | 2009 | 18590586 |
| assessment on the fermentability of xylooligosaccharides from rice husks by probiotic bacteria. | liquors from rice husk autohydrolysis, containing xylooligosaccharides (xos), other saccharides, and nonsaccharide compounds, were refined by membrane processing to increase the proportion of substituted xos in refined liquors. xos were assayed for composition and degree of polymerization (dp) distribution and hydrolyzed with commercial enzymes for obtaining xos with dp in the range of 2-6. nanofiltered, hydrolyzed liquors were subjected to ion exchange processing to yield a final product contai ... | 2008 | 18642926 |
| quantification of folic acid in human feces after administration of bifidobacterium probiotic strains. | folic acid, or vitamin b9, is involved in appropriate regulation of dna replication, synthesis of purines and deoxythymidine (dtmp), conversion of homocysteine to methionine, histidine catabolism, and correct differentiation of the neural tube during fetal organogenesis. folic acid from food sources is almost completely absorbed in the small intestine, mostly in the jejunum, and does not reach the large intestine. the administration of probiotic strains able to synthesize folates de novo and rel ... | 2008 | 18685499 |
| quantification of enterococci and bifidobacteria in georgia estuaries using conventional and molecular methods. | fecal pollution is a serious threat to the estuarine environment along the georgia coast. culture-dependant and molecular methodologies were utilized to compare and evaluate the abundance of fecal indicator bacteria in four georgia estuaries (darien river, frederica river, gulley hole creek, and st. marys river). the functionality of enterococci and bifidobacteria as indicator organisms in marine environments was assessed, as well as bifidobacterium adolescentis densities. at each study site, en ... | 2008 | 18708238 |
| anti-proliferative effects of bifidobacterium adolescentis spm0212 extract on human colon cancer cell lines. | lactic acid bacteria (lab) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as anti-tumor activity. the aim of the present work was to study the growth inhibition of tumor cells by butanol extract of bifidobacterium adolescentis isolated from healthy young koreans. | 2008 | 18950540 |
| improvement of bacterial transformation efficiency using plasmid artificial modification. | we have developed a method to improve the transformation efficiency in genome-sequenced bacteria, using 'plasmid artificial modification' (pam), using the host's own restriction system. in this method, a shuttle vector was pre-methylated in escherichia coli cells, which carry all the putative genes encoding the dna modification enzymes of the target microorganism, before electroporation was performed. in the case of bifidobacterium adolescentis atcc15703 and pkkt427 (3.9 kb e. coli-bifidobacteri ... | 2009 | 19004868 |
| linking phylogenetic identities of bacteria to starch fermentation in an in vitro model of the large intestine by rna-based stable isotope probing. | carbohydrates, including starches, are an important energy source for humans, and are known for their interactions with the microbiota in the digestive tract. largely, those interactions are thought to promote human health. using 16s ribosomal rna (rrna)-based stable isotope probing (sip), we identified starch-fermenting bacteria under human colon-like conditions. to the microbiota of the tim-2 in vitro model of the human colon 7.4 g l(-1) of [u-(13)c]-starch was added. rna extracted from lumen ... | 2009 | 19128319 |
| exploring the diversity of the bifidobacterial population in the human intestinal tract. | although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. we performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rrna gene sequences (16s rrna gene and internally transcribed spacer [its] 16s-23s spacer sequences) of isolated colonies. a total of 900 isolate ... | 2009 | 19168652 |
| altered early infant gut microbiota in children developing allergy up to 5 years of age. | early colonization with bifidobacteria and lactobacilli is postulated to protect children from allergy, while clostridium (c.) difficile colonization might be associated with allergic disease. previous studies of infant gut microbiota in relation to subsequent allergy development have mostly employed culture-dependent techniques, studied genera of bacteria and the follow-up period was limited to 2 years. | 2009 | 19220322 |
| coculture fermentations of bifidobacterium species and bacteroides thetaiotaomicron reveal a mechanistic insight into the prebiotic effect of inulin-type fructans. | four bifidobacteria, each representing a cluster of strains with specific inulin-type-fructan degradation capacities, were grown in coculture fermentations with bacteroides thetaiotaomicron lmg 11262, a strain able to metabolize both oligofructose and inulin. in a medium for colon bacteria with inulin as the sole added energy source, the ability of the bifidobacteria to compete for this substrate reflected phenotypical variation. bifidobacterium breve yakult, a strain that was not able to degrad ... | 2009 | 19251883 |
| interplay between weight loss and gut microbiota composition in overweight adolescents. | the aim of this study was to determine the influence of an obesity treatment program on the gut microbiota and body weight of overweight adolescents. thirty-six adolescents (13-15 years), classified as overweight according to the international obesity task force bmi criteria, were submitted to a calorie-restricted diet (10-40%) and increased physical activity (15-23 kcal/kg body weight/week) program over 10 weeks. gut bacterial groups were analyzed by quantitative real-time pcr before and after ... | 2009 | 19390523 |
| molecular characterization of native isolates of lactic acid bacteria, bifidobacteria and yeasts for beneficial attributes. | in a changing scenario of food habits being associated with wellness factors through the concepts of probiotics and prebiotics, an attempt has been made to characterize on molecular basis, the desirable benefits associated with natural isolates of lactic acid bacteria, bifidobacteria, and yeasts. from a diverse range of foods and related samples, based on conventional microbiological protocols, three well-characterized natural isolates of lactobacillus plantarum mtcc 5422, bifidobacterium adoles ... | 2009 | 19407995 |
| the persistence of bifidobacteria populations in a river measured by molecular and culture techniques. | to determine relative to faecal coliforms (fc) and sulfite-reducing clostridia (src), the environmental persistence of natural populations of bifidobacterium spp. enumerated by culturing and quantitative polymerase chain reaction (q-pcr). | 2009 | 19486397 |
| intra-species diversity between seven bifidobacterium adolescentis strains identified by genome-wide tiling array analysis. | six bifidobacterium adolescentis strains, jcm1275, jcm1251, jcm7044, jcm7045, jcm7046, and 9-124, were analyzed using a tiling array designed according to the full genome sequence of atcc15703. the results demonstrated deletion clusters along with single-gene mutations and deletions. most deletions concerned genes involved in polysaccharide and cell-surface biogenesis. a dendrogram illustrating the deletions and mutations is presented and the evolution of b. adolescentis is discussed. | 2009 | 19502759 |
| in vitro fermentation of arabinoxylan-derived carbohydrates by bifidobacteria and mixed fecal microbiota. | bifidobacterium adolescentis atcc 15703, bifidobacterium breve atcc 15700, bifidobacterium longum atcc 15707, and human fecal microbiota were cultivated in vitro with d-xylose, l-arabinose, xylo-oligosaccharides (xos), and arabinoxylo-oligosaccharides (axos) as carbon sources. the ph, formation of volatile fatty acids, and carbohydrate utilization profiles were followed. in the pure bifidobacteria cultures optical density and in the fecal slurries pressure and h(2) were also detected. a differin ... | 2009 | 19694435 |
| oral administration of live bifidobacterium substrains isolated from centenarians enhances intestinal function in mice. | we studied the effects of two bifidobacteria strains isolated from healthy centenarians on intestinal function in mice. bifidobacterium adolescentis bbmn23 and bifidobacterium longum bbmn68 were orally administrated to specific pathogen-free balb/c mice at different doses (2 x 10(11), 2 x 10(9), or 2 x 10(7) cfu/kg body weight) each day for 4 weeks. villus height, crypt depth, villus width, and villus/crypt ratio (v/c) were determined. the content of duodenal secreted immunoglobulin a (siga) was ... | 2009 | 19701668 |
| lactate has the potential to promote hydrogen sulphide formation in the human colon. | high concentrations of sulphide are toxic for the gut epithelium and may contribute to bowel disease. lactate is a favoured cosubstrate for the sulphate-reducing colonic bacterium desulfovibrio piger, as shown here by the stimulation of sulphide formation by d. piger dsm749 by lactate in the presence of sulphate. sulphide formation by d. piger was also stimulated in cocultures with the lactate-producing bacterium bifidobacterium adolescentis l2-32. other lactate-utilizing bacteria such as the bu ... | 2009 | 19732152 |
| microencapsulation in genipin cross-linked gelatine-maltodextrin improves survival of bifidobacterium adolescentis during exposure to in vitro gastrointestinal conditions. | to improve survival during exposure to adverse conditions, probiotic bifidobacterium adolescentis 15703t cells were encapsulated in novel mono-core and multi-core phase-separated gelatine-maltodextrin (gmd) microspheres where the gelatine (g) phase was cross-linked with genipin (gp). microscopy showed that encapsulated cells were exclusively associated with maltodextrin (md) core(s). small (average diameter 37 microm) and large (70 microm) gmd and g microspheres were produced by modulating facto ... | 2010 | 19860547 |
| human-specific fecal bacteria in wastewater treatment plant effluents. | the objective of this study was to identify fecal bacteria able to persist after wastewater treatment and that could be used as indicators of human fecal contamination. in a first step, the diversity of bacteroidales, clostridiaceae, bifidobacterium, and bacillus-streptococcus-lactobacillus cluster (bsl) was analysed using a fingerprint technique (ce-sscp) and 16s rdna libraries in waters collected at the end of the treatment process in different urban wastewater treatment plants. for each group ... | 2010 | 19945729 |
| microcalorimetric assay on the antimicrobial property of five hydroxyanthraquinone derivatives in rhubarb (rheum palmatum l.) to bifidobacterium adolescentis. | it was found that the intestinal bacteria balance would be deteriorated by rhubarb especially in long term treatment. bifidobacteria is one of the most common species of probiotics in human intestine. the suppression of this particular probiotic, such as bifidobacterium adolescentis, one of the dominant anaerobes in the intestines of humans, might lead to imbalance of intestinal flora and is considered to be potentially riskful for human health. hence, the inhibitory effects of the five main com ... | 2010 | 19962872 |
| bifidobacterium stercoris sp. nov., isolated from human faeces. | strain eg1(t), an anaerobic, gram-stain-positive, non-motile and non-spore-forming bacterium, was isolated from human faeces. the optimal temperature for growth was 37 °c and tests for oxidase and catalase activities gave negative results. fructose-6-phosphate phosphoketolase activity was detected. acid was produced during fermentation of several substrates, including glucose. the end products of glucose fermentation were acetic acid and lactic acid, which were produced in a molar ratio o ... | 2010 | 20081020 |
| bifidobacterium adolescentis supplementation ameliorates parenteral nutrition-induced liver injury in infant rabbits. | parenteral nutrition (pn)-induced liver injury is associated with gut atrophy, and probiotics have demonstrated the ability to stabilize the intestinal microecosystem and offer protection against bacterial translocation from the gut to the liver. therefore, we hypothesized that enteral bifidobacterium supplements could alleviate pn-associated liver injury. | 2010 | 20094783 |
| molecular indicators used in the development of predictive models for microbial source tracking. | a number of chemical, microbial, and eukaryotic indicators have been proposed as indicators of fecal pollution sources in water bodies. no single one of the indicators tested to date has been able to determine the source of fecal pollution in water. however, the combined use of different indicators has been demonstrated to be the best way of defining predictive models suitable for determining fecal pollution sources. molecular methods are promising tools that could complement standard microbiolo ... | 2010 | 20118380 |
| characterization of recombinant beta-fructofuranosidase from bifidobacterium adolescentis g1. | we have previously reported on purification and characterization of beta-fructofuranosidase (beta-ffase) from bifidobacterium adolescentis g1. this enzyme showed high activity of hydrolysis on fructo-oligosaccharides with a low degree of polymerization. recently, genome sequences of b. longum ncc2705 and b. adolescentis atcc 15703 were determined, and csca gene in the both genome sequences encoding beta-ffase was predicted. here, cloning of csca gene encoding putative beta-ffase from b. adolesce ... | 2010 | 20380746 |
| effect of mucilage from yam on activation of lymphocytic immune cells. | the immunostimulating activities of mucilage fraction from yam were investigated. the proliferation of bsa-primed lymph node cells was enhanced between 4.1- to 10.9-fold compare to control, when cultured with 1 to 25 microg/ml of yam-mucilage fraction. it showed strong immunopotentiating activity than ginseng extract and as remarkable as bifidobacterium adolescentis m101-4 known as a positive immunostimulator. mitogenicity to lymph node cells was fully induced by concanavalin a and lipopolysacch ... | 2007 | 20535393 |
| functional analysis of glycoside hydrolase family 8 xylanases shows narrow but distinct substrate specificities and biotechnological potential. | the potential of glycoside hydrolase family (gh) 8 xylanases in biotechnological applications is virtually unexplored. therefore, the substrate preference and hydrolysis product profiles of two gh8 xylanases were evaluated to investigate their activities and substrate specificities. a gh8 xylanase from an uncultured bacterium (rxyn8) shows endo action but very selectively releases xylotriose from its substrates. it has a higher activity than the pseudoalteromonas haloplanktis gh8 endo-xylanase ( ... | 2010 | 20552357 |
| amoxicillin treatment modifies the composition of bifidobacterium species in infant intestinal microbiota. | amoxicillin is a beta-lactam antibiotic largely used in childhood. however only few studies described its impact on composition of children gut microbiota, in particular on bifidobacterium populations considered as beneficial microorganisms. in this study, the impact on faecal bifidobacterium species of a seven-day amoxicillin treatment was quantitatively and qualitatively assessed in infants during an episode of acute respiratory infection. | 2010 | 20601031 |
| dissimilarity in the occurrence of bifidobacteriaceae in vaginal and perianal microbiota in women with bacterial vaginosis. | recent data point at the similarity between the perianal and vaginal microflora in terms of lactobacillus species involved. bacterial vaginosis, the most common perturbation of the vaginal microflora involving primarily overgrowth of gardnerella vaginalis, has also been suggested to involve a recto-vaginal pathway. we addressed this issue with regard to bacteria of the bifidobacteriaceae family. in particular, we investigated the putative concordance of the presence of g. vaginalis and a series ... | 2010 | 20620215 |
| increasing the thermostability of sucrose phosphorylase by multipoint covalent immobilization. | sucrose phosphorylase from bifidobacterium adolescentis was recombinantly expressed in escherichia coli and purified by use of a his-tag. kinetic characterization of the enzyme revealed an optimal temperature for phosphorolytic activity of 58°c, which is surprisingly high for an enzyme from a mesophilic source. the temperature optimum could be further increased to 65°c by multipoint covalent immobilization on sepabeads ec-hfa. the optimal immobilization conditions were determined by surface resp ... | 2010 | 20691225 |
| new l-amino acid ligases catalyzing oligopeptide synthesis from various microorganisms. | l-amino acid ligase synthesizes various peptides from unprotected l-amino acids in an atp-dependent manner. known l-amino acid ligases catalyze only dipeptide synthesis, but recently we found that rizb of bacillus subtilis nbrc 3134 catalyzes oligopeptide synthesis. in the present study, we searched for new members of the l-amino acid ligase group that catalyze oligopeptide synthesis. several hypothetical proteins possessing the atp-grasp motif were selected by in silico analysis. these recombin ... | 2010 | 20699590 |
| continuous culture of human faecal bacteria as an in vitro model for the colonic microflora. | to investigate the role of human gut bacteria in the metabolism of potentially reactive compounds we have developed an in vitro model of the human faecal microflora using a two-stage continuous culture inoculated with human faeces. the cultured bacterial population retained many of the bacteriological and biochemical characteristics of the flora present in the faecal sample used for inoculation. obligate anaerobes were the predominant bacterial types found in vitro and included bacteroides ovatu ... | 1990 | 20702225 |
| development of microbial and chemical mst tools to identify the origin of the faecal pollution in bathing and shellfish harvesting waters in france. | the microbiological quality of coastal or river waters can be affected by faecal pollution from human or animal sources. an efficient mst (microbial source tracking) toolbox consisting of several host-specific markers would therefore be valuable for identifying the origin of the faecal pollution in the environment and thus for effective resource management and remediation. in this multidisciplinary study, after having tested some mst markers on faecal samples, we compared a selection of 17 param ... | 2010 | 20709349 |
| comparison of four polymerase chain reaction methods for the rapid detection of human fecal pollution in marine and inland waters. | we compared the effectiveness of three pcr protocols for the detection of bifidobacterium adolescentis and one pcr protocol for detecting bacteroidales as indicators of human fecal pollution in environmental samples. quantitative pcr indicated that a higher concentration of b. adolescentis dna was recovered from sewage samples on the 0.2 mum filters compared to the 0.45 mum filters, and there was no evidence of qpcr inhibitors in the dna extracts. with the matsuki method (1999), b. adolescentis ... | 2010 | 20811614 |
| sucrose phosphorylase as cross-linked enzyme aggregate: improved thermal stability for industrial applications. | sucrose phosphorylase is an interesting biocatalyst that can glycosylate a variety of small molecules using sucrose as a cheap but efficient donor substrate. the low thermostability of the enzyme, however, limits its industrial applications, as these are preferably performed at 60°c to avoid microbial contamination. cross-linked enzyme aggregates (cleas) of the sucrose phosphorylase from bifidobacterium adolescentis were found to have a temperature optimum that is 17°c higher than that of the so ... | 2010 | 20872729 |
| development and pilot evaluation of a novel probiotic mixture for the management of seasonal allergic rhinitis. | microbial exposure may direct the immune system away from allergic-type responses, but until now probiotic interventions have had limited success in the prevention and treatment of allergic diseases. in this study, a novel probiotic mixture was specifically created based on preliminary in vitro investigations on pollen-induced immune responses. a mixture with lactobacillus rhamnosus gr-1 and a novel fecal bifidobacterium adolescentis isolate was formulated into a yogurt and tested for its effect ... | 2010 | 20921983 |
| substrate specificity of three recombinant α-l-arabinofuranosidases from bifidobacterium adolescentis and their divergent action on arabinoxylan and arabinoxylan oligosaccharides. | bifidobacterium adolescentis possesses several arabinofuranosidases able to hydrolyze arabinoxylans (ax) and ax oligosaccharides (axos), the latter being bifidogenic carbohydrates with potential prebiotic properties. we characterized two new recombinant arabinofuranosidases, abfa and abfb, and axh-d3, a previously studied arabinofuranosidase from b. adolescentis. abfa belongs to glycoside hydrolase family (gh) 43 and removed arabinose from the c(o)2 and c(o)3 position of monosubstituted xylose r ... | 2010 | 20971079 |
| intestinal bacteroides species associated with coeliac disease. | to characterise the predominant species of bacterial populations associated with duodenal biopsies of paediatric patients with active and treated coeliac disease. | 2010 | 20972239 |
| evaluation of physical, chemical and microbiological parameters of water quality in the harris neck estuarine marshes along the georgia coast. | analysis of the physical, chemical and biological parameters assessing water quality in harris neck estuary indicated that the average dissolved oxygen level was 8.6 mg/l, it maintained moderate levels of total dissolved nitrogen (2.7-4.6 mg/l) and total dissolved phosphorous (<0.05 mg/l), chlorophyll a was above 5.0 μg/l and it is contaminated with low levels of fecal bacteria. bifidobacterium adolescentis, a putative marker of human fecal pollution, was detected once at stations 3 and 5. overa ... | 2010 | 21122879 |
| high-throughput method for comparative analysis of denaturing gradient gel electrophoresis profiles from human fecal samples reveals significant increases in two bifidobacterial species after inulin-type prebiotic intake. | denaturing gradient gel electrophoresis (dgge) is one of the most commonly used molecular tools to study complex microbial communities. despite its widespread use, meaningful interpretative analysis remains a major drawback of this method. we evaluated the combination of computer-assisted band-matching with nonparametric statistics for comparative analysis of dgge banding patterns. fecal samples from 17 healthy volunteers who consumed 20 g of the prebiotic compound oligofructose-enriched inulin ... | 2010 | 21133959 |
| resistant starches types 2 and 4 have differential effects on the composition of the fecal microbiota in human subjects. | to systematically develop dietary strategies based on resistant starch (rs) that modulate the human gut microbiome, detailed in vivo studies that evaluate the effects of different forms of rs on the community structure and population dynamics of the gut microbiota are necessary. the aim of the present study was to gain a community wide perspective of the effects of rs types 2 (rs2) and 4 (rs4) on the fecal microbiota in human individuals. | 2010 | 21151493 |
| predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria lactobacillus acidophilus ncfm and bifidobacterium animalis subsp. lactis bi-07. | the effect of probiotic bacteria lactobacillus acidophilus ncfm and bifidobacterium lactis bi-07 on the composition of the lactobacillus group, bifidobacterium and the total bacterial population in feces from young children with atopic dermatitis was investigated. the study included 50 children randomized to intake of one of the probiotic strain or placebo. microbial composition was characterized by denaturing gradient gel electrophoresis, quantitative pcr and, in a subset of subjects, by pyrose ... | 2011 | 21204871 |
| dysbiosis of the faecal microbiota in patients with crohn's disease and their unaffected relatives. | a general dysbiosis of the intestinal microbiota has been established in patients with crohn's disease (cd), but a systematic characterisation of this dysbiosis is lacking. therefore the composition of the predominant faecal microbiota of patients with cd was studied in comparison with the predominant composition in unaffected controls. whether dysbiosis is present in relatives of patients cd was also examined. | 2011 | 21209126 |
| celiac disease in patients with type 1 diabetes: a condition with distinct changes in intestinal immunity? | two common chronic childhood diseases-celiac disease (cd) and type 1 diabetes (t1d)-result from complex pathological mechanisms where genetic susceptibility, environmental exposure, alterations in intestinal permeability and immune responses play central roles. in this study, we investigated whether these characteristics were universal for cd independently of t1d association. for this purpose, we studied 36 children with normal small-bowel mucosa and 26 children with active cd, including 12 pati ... | 2011 | 21317917 |
| antimicrobial activity of bifidobacterium spp. isolated from healthy adult koreans against cariogenic microflora. | objective: dental caries is the main common infectious disease in the human oral cavity. streptococcus mutans and streptococcus sobrinus were reported to be the most important etiological factors in human dental caries. thus, we examined the inhibitory effects of bifidobacterium spp. cells and culture supernatants against s. mutans and s. sobrinus, including streptococcus gordonii, and aggregatibacter actinomycetemcomitans, which is associated with periodontal disease. methods: mutans streptococ ... | 2011 | 21439550 |
| crystal structure of a metal-dependent phosphoesterase (yp_910028.1) from bifidobacterium adolescentis: computational prediction and experimental validation of phosphoesterase activity. | the crystal structures of an unliganded and adenosine 5'-monophosphate (amp) bound, metal-dependent phosphoesterase (yp_910028.1) from bifidobacterium adolescentis are reported at 2.4 and 1.94 å, respectively. functional characterization of this enzyme was guided by computational analysis and then confirmed by experiment. the structure consists of a polymerase and histidinol phosphatase (php, pfam: pf02811) domain with a second domain (residues 105-178) inserted in the middle of the php sequence ... | 2011 | 21538547 |
| role of metabolism by intestinal bacteria in arbutin-induced toxicity in vitro. | a possible role of metabolism by intestinal bacteria in arbutin-induced toxicity was investigated in mammalian cell cultures. following an incubation of arbutin with intestinal bacteria, either bifidobacterium longum hy81 or bifidobacterium adolescentis, for 24 h, its aglycone hydroquinone could be produced and detected in the bacterial culture media. the bacterial growth was not affected up to 10 mm arbutin in the culture medium. when the toxicity of bacteria cultured medium with arbutin was te ... | 2011 | 21544735 |
| fermentation of β-glucans derived from different sources by bifidobacteria: evaluation of their bifidogenic effect. | β-glucans obtained from barley, seaweed, bacteria, and mushroom sclerotia were incubated with pure cultures of bifidobacterium infantis , bifidobacterium longum , and bifidobacterium adolescentis for a 24 h batch fermentation to evaluate their bifidogenic effect with inulin as the positive control. the ph value in all culture media was decreased by 0.5-1.5 units. all β-glucans supported the growth of the three bifidobacteria with b. infantis, having a relatively larger increase in populations (3 ... | 2011 | 21568326 |
| evaluation of microbiological water quality in the pettaquamscutt river (rhode island, usa) using chemical, molecular and culture-dependent methods. | we evaluated microbiological water quality in the pettaquamscutt river (rhode island, usa), an estuarine river. fecal coliform (fc) and enterococci (fe) bacteria, presence of bifidobacterium adolescentis dna (indicating human fecal contamination), and optical brightener (ob) fluorescence (associated with laundry detergents) were determined for 14 stations from may to september 2010. six stations had high counts of fe and fc, and the presence of b. adolescentis dna and high ob fluorescence indica ... | 2011 | 21570698 |