| cloning and nucleotide sequence of the inulin fructotransferase (dfa i-producing) gene of arthrobacter globiformis s14-3. | a gene encoding an inulin fructotransferase (dfa i-producing) [ec 2.4.1.200] from arthrobacter globiformis s14-3 was cloned and the nucleotides sequenced, for the first time. the sequence indicated that the native enzyme protein is composed of 392 amino acid residues. the native enzyme is an extracellar enzyme produced in the culture supernatant of a. globiformis s14-3, but the nucleotide sequence of the gene lacks a sequence for signal peptide for secretion. the 1.5-kb dna fragment encoding the ... | 1995 | 8534968 |
| manganese(ii)-dependent extradiol-cleaving catechol dioxygenase from arthrobacter globiformis cm-2. | a manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from arthrobacter globiformis strain cm-2 (mndd) cloned in escherichia coli has been purified to homogeneity. sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 da). steady-state kinetic analysis of mndd with a variety of substrates and inhibitors yields very similar relative rates to the known fe(ii)- and mn(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenases from pseudomon ... | 1996 | 8555170 |
| transformation of synechococcus with a gene for choline oxidase enhances tolerance to salt stress. | choline oxidase, isolated from the soil bacterium arthrobacter globiformis, converts choline to glycinebetaine (n-trimethylglycine) without a requirement for any cofactors. the gene for this enzyme, designated coda, was cloned and introduced into the cyanobacterium synechococcus sp. pcc 7942. the coda gene was expressed under the control of a strong constitutive promoter, and the transformed cells accumulated glycinebetaine at intracellular levels of 60-80 mm. consequently the cells acquired tol ... | 1995 | 8555454 |
| comparison of 16s ribosomal rna genes in clavibacter michiganensis subspecies with other coryneform bacteria. | nearly complete sequences (97-99%) of the 16s rrna genes were determined for type strains of clavibacter michiganensis subsp. michiganensis, clavibacter michiganensis subsp. insidiosus, clavibacter michiganensis subsp. sepedonicus, and clavibacter michiganensis subsp. nebraskensis. the four subspecies had less than 1% dissimilarity in their 16s rrna genes. comparative studies indicated that the c. michiganensis subsp. shared relatively high homology with the 16s rrna gene of clavibacter xyli. fu ... | 1995 | 8590406 |
| biosynthesis of topa quinone cofactor in bacterial amine oxidases. solvent origin of c-2 oxygen determined by raman spectroscopy. | resonance raman spectroscopy is an excellent technique for providing structural information on the 2,4, 5-trihydroxyphenylalanine quinone (tpq) cofactor in copper-containing amine oxidases. this technique has been used to investigate the copper- and o2-dependent biosynthesis of the tpq cofactor in phenylethylamine oxidase (peao) and histamine oxidase from arthrobacter globiformis. incubation of the holoenzyme in h218o causes frequency shifts at 1684(-26) cm-1 in peao and at 1679(-28) cm-1 in his ... | 1996 | 8617737 |
| cold shock and cold acclimation proteins in the psychrotrophic bacterium arthrobacter globiformis si55. | the psychrotrophic bacterium arthrobacter globiformis si55 was grown at 4 and 25 degrees c, and the cell protein contents were analyzed by two-dimensional electrophoresis. cells subjected to cold shocks of increasing magnitude were also analyzed. correspondence analysis of protein appearance distinguished four groups of physiological significance. group i contained cold shock proteins (csps) overexpressed only after a large temperature downshift. group ii contained csps with optimal expression a ... | 1996 | 8655472 |
| role of conserved asn-tyr-asp-tyr sequence in bacterial copper/2,4, 5-trihydroxyphenylalanyl quinone-containing histamine oxidase. | copper amine oxidase contains a covalently bound quinonoid cofactor, 2,4,5-trihydroxyphenylalanyl quinone (tpq), which is synthesized by post-translational modification of a specific tyrosyl residue occurring in the highly conserved sequence, asn-tyr-(asp/glu)-tyr. to elucidate the role(s) of the conserved sequence in the biogenesis of tpq, each of the corresponding residues at positions 401-404 in the recombinant histamine oxidase from arthrobacter globiformis has been replaced with other amino ... | 1996 | 8798429 |
| the action in vivo of glycine betaine in enhancement of tolerance of synechococcus sp. strain pcc 7942 to low temperature. | the cyanobacterium synechococcus sp. strain pcc 7942 was transformed with the coda gene for choline oxidase from arthrobacter globiformis under the control of a constitutive promoter. this transformation allowed the cyanobacterial cells to accumulate glycine betaine at 60 to 80 mm in the cytoplasm. the transformed cells could grow at 20 degrees c, the temperature at which the growth of control cells was markedly suppressed. photosynthesis of the transformed cells at 20 degrees c was more toleran ... | 1997 | 8990284 |
| molecular cloning of an inulin fructotransferase (depolymerizing) gene from arthrobacter sp. h65-7 and its expression in escherichia coli. | the gene encoding an extracellular inulin fructotransferase (depolymerizing) (inulase ii) (ec 2.4.1.93), designated ift gene, was cloned from the genomic dna of arthrobacter sp. h65-7, and expressed in escherichia coli for the first time. sequence analysis showed a single open reading frame consisting of 1314 base pairs that encoded a signal peptide of 32 amino acids and a mature protein of 405 amino acids. the primary structure showed a homology of 49.8% with that of the inulin fructotransferas ... | 1997 | 9028036 |
| mechanistic studies of topa quinone biogenesis in phenylethylamine oxidase. | an alternative purification for apophenylethylamine oxidase from arthrobacter globiformis has been developed, which avoids the use of possible contaminants that may interfere with the topa quinone (tpq) self-processing reaction. the binding of cu(ii) and the kinetics of tpq formation in these enzyme preparations have been reinvestigated. our results show that cu(ii) is not significantly reduced when added to the apoprotein under anaerobic conditions. the cu(ii) epr and circular dichroism spectra ... | 1997 | 9047291 |
| manganese(ii) active site mutants of 3,4-dihydroxyphenylacetate 2,3-dioxygenase from arthrobacter globiformis strain cm-2. | whereas all other members of the extradiol-cleaving catechol dioxygenase family are iron-dependent, the 3,4-dihydroxyphenylacetate 2,3-dioxygenase (mndd) from arthrobacter globiformis cm-2 is dependent on manganese for catalytic activity. recently, the endogenous iron ligands of one family member, the 2,3-dihydroxybiphenyl 1,2-dioxygenase (bphc), were identified crystallographically as two histidines and a glutamic acid [sugiyama, k., et al. (1995) proc. jpn. acad., ser. b 71, 32-35; han, et al. ... | 1997 | 9047314 |
| is1473, a putative insertion sequence identified in the plasmid pao1 from arthrobacter nicotinovorans: isolation, characterization, and distribution among arthrobacter species. | a putative insertion sequence (is1473) has been cloned and sequenced. the 1087-bp element was found between the moaa and the ndha genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pao1 plasmid of arthrobacter nicotinovorans. it is flanked by an imperfect repeat of 33 bp and carries two overlapping open reading frames which, by programmed -1 translational frameshifting, may produce a transposase of 36.735 da with a pi = 10. 18. the deduced protein is similar to ... | 1997 | 9073580 |
| molecular characterization of the bet genes encoding glycine betaine synthesis in sinorhizobium meliloti 102f34. | as a first step towards the elucidation of the molecular mechanisms responsible for the utilization of choline and glycine betaine (betaine) either as carbon and nitrogen sources or as osmoprotectants in sinorhizobium meliloti, we selected a tn5 mutant, lts23-1020, which failed to grow on choline but grew on betaine. the mutant was deficient in choline dehydrogenase (cdh) activity, failed to oxidize [methyl-14c]choline to [methyl-14c]betaine, and did not use choline, but still used betaine, as a ... | 1997 | 9141699 |
| the species composition of oligotrophic bacterial communities of lake balaton--a numerical analysis. | from among the 565 aerobic and facultatively anaerobic, oligotrophic bacterial isolated obtained from the open water region of the balatonfüred-bay, 58 representative strains were selected for detailed taxonomic-physiological studies and numerical analyses as well as simultaneous comparisons with authentic bacterial strains isolated earlier by langó (1987) from different water habitat of the lake balaton. on the basis of 186 ceded differential diagnostic characteristics involved in these analyse ... | 1996 | 9147724 |
| cloning, overexpression, and mutagenesis of the gene for homoprotocatechuate 2,3-dioxygenase from brevibacterium fuscum. | homoprotocatechuate (hpca, 3,4-dihydroxyphenylacetate) is a central intermediate for the bacterial degradation of aromatic compounds. homoprotocatechuate 2,3-dioxygenase (hpcd) catalyzes the key ring cleavage step in the metabolism of hpca by the gram (+) bacterium brevibacterium fuscum to yield alpha-hydroxy-delta-carboxymethyl cis-muconic semialdehyde. a genomic dna library of b. fuscum was constructed in escherichia coli using a cosmid vector and screened by spraying the cells with hpca. one ... | 1997 | 9179284 |
| transformation of arabidopsis thaliana with the coda gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress. | glycinebetaine is one of the compatible solutes that accumulate in the chloroplasts of contain halotolerant plants when these plants are exposed to salt or cold stress. the coda gene for choline oxidase, the enzyme that converts choline into glycinebetaine, has previously been cloned from a soil bacterium, arthrobacter globiformis. transformation of arabidopsis thaliana with the cloned coda gene under the control of the 35s promoter of cauliflower mosaic virus enabled the plant to accumulate gly ... | 1997 | 9263456 |
| capa, a cspa-like gene that encodes a cold acclimation protein in the psychrotrophic bacterium arthrobacter globiformis si55. | by use of arthrobacter globiformis si55, a psychrotrophic bacterium capable of growth between -5 and +32 degrees c, we cloned and sequenced capa, a gene homologous to cspa encoding the major cold shock protein in escherichia coli. the deduced protein sequence has a high level of identity with the sequences of other cspa-related proteins from various sources, and no particular residue or domain that could be specific to cold-adapted microorganisms emerged. we show that capa was produced very rapi ... | 1997 | 9294420 |
| topaquinone-dependent amine oxidases: identification of reaction intermediates by raman spectroscopy. | resonance raman (rr) spectroscopy has proven to be an excellent technique for providing structural information about the 2,4, 5-trihydroxyphenylalaninequinone (tpq) cofactor and for identifying the source of oxygen atoms during the posttranslational synthesis of the cofactor. through specific labeling of the c2, c4, and c5 oxygens of tpq in phenylethylamine oxidase (peao) from arthrobacter globiformis, we have identified the c=o stretch of the c5 carbonyl at 1683 cm-1 (-27 in 18o) and the c=o st ... | 1997 | 9298968 |
| crystal structures of the copper-containing amine oxidase from arthrobacter globiformis in the holo and apo forms: implications for the biogenesis of topaquinone. | the crystal structures of the copper enzyme phenylethylamine oxidase from the gram-positive bacterium arthrobacter globiformis (agao) have been determined and refined for three forms of the enzyme: the holoenzyme in its active form (at 2.2 a resolution), the holoenzyme in an inactive form (at 2.8 a resolution), and the apoenzyme (at 2.2 a resolution). the holoenzyme has a topaquinone (tpq) cofactor formed from the apoenzyme by the post-translational modification of a tyrosine residue in the pres ... | 1997 | 9405045 |
| asking for the indicator function of bioassays evaluating soil contamination: are bioassay results reasonable surrogates of effects on soil microflora? | in evaluating the biological effect of solid materials like soil a bacterial contact assay often shows higher sensitivity than elutriate testing. results of the bacillus cereus contact assay for some environmental important toxicants are presented in this article. a comparison with another heterotrophic soil bacterium, arthrobacter globiformis, shows comparable sensitivity. in a bioassay approach organisms at the level of individuals or populations are exposed to soil material to determine the s ... | 1998 | 9493327 |
| biochemical synthesis of several chiral insecticide intermediates and mechanisms of action of relevant enzymes. | efficient biochemical processes were developed for the synthesis of several chiral alcohol and acid intermediates of insecticides by a combination of strictly stereoselective hydrolytic enzyme-catalyzed reactions and subsequent chemical transformations with inversion or racemization of the chiral center of the undesired antipodes. the whole amounts of starting racemic mixtures are converted to desired stereoisomers in the processes, which are generally applicable to the industrial productions of ... | 1998 | 9501511 |
| demethylation of acridine orange by arthrobacter globiformis. | | 1998 | 9595195 |
| intraspecific variation of unusual phospholipids from corynebacterium spp. containing a novel fatty acid. | the novel fatty acid trans-9-methyl-10-octadecenoic acid was isolated from the coryneform bacterial strain lmg 3820 (previously misidentified as arthrobacter globiformis) and identified by spectroscopic methods and chemical derivatization. this fatty acid is attached to the unusual lipid acyl phosphatidylglycerol. five different species of this lipid type were identified; their structures were elucidated by tandem mass spectrometry and are reported here for the first time. additionally, we ident ... | 1998 | 9721308 |
| exploring a channel to the active site of copper/topaquinone-containing phenylethylamine oxidase by chemical modification and site-specific mutagenesis. | copper amine oxidase contains an organic redox cofactor, 2,4, 5-trihydroxyphenylalaninequinone (topaquinone, tpq), derived by the post-translational modification of a specific tyrosyl residue. to identify amino acid residues participating in the biogenesis of tpq in the recombinant phenylethylamine oxidase from arthrobacter globiformis, we have modified the copper/tpq-less apoenzyme and the copper/tpq-containing holoenzyme with 4-fluoro-7-nitrobenzo-2-oxa-1, 3-diazole (nbd-f). in the apoenzyme m ... | 1998 | 9760229 |
| enhancement of the tolerance of arabidopsis to high temperatures by genetic engineering of the synthesis of glycinebetaine. | arabidopsis thaliana was transformed with the coda gene for choline oxidase from arthrobacter globiformis under control of the 35s rna promoter of cauliflower mosaic virus. as a result, high levels of glycinebetaine accumulated in the seeds of transformed plants. transformation with the coda gene significantly enhanced the tolerance to high temperatures during the imbibition and germination of seeds, as well as during growth of young seedlings. the extent of enhancement of the tolerance to high ... | 1998 | 9839462 |
| allergic alveolitis among agricultural workers in eastern poland: a study of twenty cases. | the aim of this study was to identify the specific agents which caused extrinsic allergic alveolitis (eaa) in the selected group of 20 agricultural workers from eastern poland. the microbiological analysis of the samples of plant materials or dusts reported by the patients as causing symptoms has been carried out, followed by allergological tests (inhalation challenge, agar-gel precipitation test, inhibition of leukocyte migration, skin test) with extrinsic microbial antigens. it was found that ... | 1998 | 9852490 |
| metabolic engineering of rice leading to biosynthesis of glycinebetaine and tolerance to salt and cold. | genetically engineered rice (oryza sativa l.) with the ability to synthesize glycinebetaine was established by introducing the coda gene for choline oxidase from the soil bacterium arthrobacter globiformis. levels of glycinebetaine were as high as 1 and 5 micromol per gram fresh weight of leaves in two types of transgenic plant in which choline oxidase was targeted to the chloroplasts (chlcod plants) and to the cytosol (cytcod plants), respectively. although treatment with 0.15 m nacl [corrected ... | 1998 | 9869407 |
| stoichiometry of the topa quinone biogenesis reaction in copper amine oxidases. | the stoichiometry of the topa quinone biogenesis reaction in phenylethylamine oxidase from arthrobacter globiformis (agao) has been determined. we have shown that the 6e- oxidation of tyrosine to topa quinone (tpq) consumes 2 mol of o2 and produces 1 mol of h2o2/mol of tpq formed. the rate of h2o2 production is first-order (kobs = 1.0 +/- 0.2 min-1), a rate only slightly lower than the rate of tpq formation directly determined previously (kobs = 1.5 +/- 0.2 min-1). this gives the following net r ... | 1999 | 10074341 |
| removal of nitrate from industrial wastewaters in a pilot plant by nitrate-tolerant klebsiella oxytoca cect 4460 and arthrobacter globiformis cect 4500 | two strains, a gram-negative bacterium klebsiella oxytoca cect 4460 and a gram-positive, mycelium-forming bacterium arthrobacter globiformis cect 4500, tolerant to up to 1 m nitrate, were isolated from the grounds of a munitions factory. under strict aerobic conditions and with appropriate c-sources, growth of these bacteria took place when the nitrate concentration in the medium was below 150 mm. optimal growth conditions regarding the culture medium composition for the biological removal of ni ... | 1998 | 10099287 |
| construction and characterization of two recombinant bacteria that grow on ortho- and para-substituted chlorobiphenyls. | cloning and expression of the aromatic ring dehalogenation genes in biphenyl-growing, polychlorinated biphenyl (pcb)-cometabolizing comamonas testosteroni vp44 resulted in recombinant pathways allowing growth on ortho- and para-chlorobiphenyls (cbs) as a sole carbon source. the recombinant variants were constructed by transformation of strain vp44 with plasmids carrying specific genes for dehalogenation of chlorobenzoates (cbas). plasmid pe43 carries the pseudomonas aeruginosa 142 ohb genes codi ... | 1999 | 10224015 |
| biochemical and phylogenetic analyses of psychrophilic isolates belonging to the arthrobacter subgroup and description of arthrobacter psychrolactophilus, sp. nov. | during our work on psychrophilic microorganisms we obtained a large collection of new isolates. in order to identify six of these, we examined their growth properties, cell wall compositions, and their 16s rrna gene sequences. the results showed that all of the isolates are gram-positive, aerobic, contain lysine in their cell walls, and belong to the high mol% g+c arthrobacter subgroup. phylogenetic analysis of the 16s rrna genes grouped five isolates obtained from a small geographical region in ... | 1999 | 10369891 |
| enhanced tolerance to light stress of transgenic arabidopsis plants that express the coda gene for a bacterial choline oxidase. | arabidopsis thaliana was transformed with the coda gene from arthrobacter globiformis. this gene encodes choline oxidase, an enzyme that converts choline to glycinebetaine. the photosynthetic activity, monitored in terms of chlorophyll fluorescence, of transformed plants was more tolerant to light stress than that of wild-type plants. this enhanced tolerance to light stress was caused by acceleration of the recovery of the photosystem ii (ps ii) complex from the photo-inactivated state. the tran ... | 1999 | 10412906 |
| adaptive characteristics of salt-induced myceloids of arthrobacter globiformis. | when arthrobacter globiformis is grown in medium containing increased concentrations of nacl or decreased levels of cations, the bacteria grow as clusters of branching myceloid cells. the sensitivities of salt-induced and citrate-induced myceloids to several environmental stresses were compared to those of normal exponential-phase bacilli and stationary-phase cocci. salt-induced myceloids were more resistant than normal cells to ultraviolet light or heat shock at 45 degrees c but not to osmotic ... | 1999 | 10510721 |
| cloning and sequence analysis of the gene for glucodextranase from arthrobacter globiformis t-3044 and expression in escherichia coli cells. | the gld gene for glucodextranase from arthrobacter globiformis t-3044 was cloned by using a combination of gene walking and probe methods and expressed on the recombinant plasmid pgd8, which was constructed with puc118, in escherichia coli cells. the enzyme gene consisted of a unique open reading frame of 3,153 bp. the comparison of the dna sequence data with the n-terminal and 6 internal amino acid sequences of the purified enzyme secreted from a. globiformis t-3044 suggested the enzyme was tra ... | 1999 | 10664850 |
| structural analysis of the elongation factor g protein from the low-temperature-adapted bacterium arthrobacter globiformis si55. | the first structural analysis of elongation factor g (ef-g) from a cold-adapted bacterium is presented. ef-g is an essential protein involved in the elongation process during protein synthesis and is therefore thought to play a crucial role in the low-temperature adaptation of cold-adapted microorganisms. to define its importance, the ef-g gene (fus) from the psychrotolerant bacterium arthrobacter globiformis si55 was cloned and sequenced. the deduced primary structure of the elongation factor i ... | 2000 | 10805567 |
| transformation of arabidopsis with the coda gene for choline oxidase enhances freezing tolerance of plants. | arabidopsis thaliana was transformed with the coda gene from arthrobacter globiformis, which encodes choline oxidase, the enzyme that synthesizes glycinebetaine from choline. the transformation enabled the plants to accumulate glycinebetaine in chloroplasts, and significantly enhanced the freezing tolerance of plants. furthermore, the photosynthetic machinery of transformed plants was more tolerant to freezing stress than that of wild-type plants. exogenous application of glycinebetaine also inc ... | 2000 | 10849360 |
| comparison of colony lift with direct spotting methods of blot preparation on the effect of colony hybridization in the detection of environmental organisms. | nucleic acid probes are used on site to detect or to identify individual microbial cells without cultivation. this molecular technique can avoid some limitations of traditional identification methods including time consuming and imprecise. this study examined the factors affecting colony hybridization and compared the effectiveness of membrane prepared by colony lifting with direct spotting procedures using the universal probe eub 338. the results of hybridization varied depending on the type of ... | 2000 | 10917884 |
| [reproductive resting forms of arthrobacter globiformis]. | submerged cultures of arthrobacter globiformis grown in media unbalanced with respect to carbon and nitrogen sources were found to contain cells exhibiting features typical of resting forms: long-term viability, specific ultrastructure, dormant metabolism, and thermoresistance. such cells were produced not only in the collection strain vkm b-1112, but also in the a. globiformis strains isolated from 2- to 3-million-year-old permafrost sediments. | 2000 | 10920808 |
| [formation of resting forms of arthrobacter globiformis in autolysing cell suspensions]. | under conditions of spontaneous or induced autolysis of thick cell suspensions, arthrobacter globiformis strains produced cells exhibiting features typical of resting microbial forms. the number of viable resting cells was greater under conditions of induced rather than spontaneous autolysis. the thermoresistance of the resting cells of a. globiformis strains isolated from 2- to 3 million-year-old permafrost was higher than that of the collection a. globiformis strain. | 2000 | 10920809 |
| genetic engineering of glycinebetaine synthesis in plants: current status and implications for enhancement of stress tolerance. | metabolic acclimation via the accumulation of compatible solutes is regarded as a basic strategy for the protection and survival of plants in extreme environments. certain plants accumulate significant amounts of glycinebetaine (betaine), a compatible quaternary amine, in response to high salinity, cold and drought. it is likely that betaine is involved in the protection of macrocomponents of plant cells, such as protein complexes and membranes, under stress conditions. genetic engineering of th ... | 2000 | 10938798 |
| enzymatic assay of histamine by amperometric detection of h2o2 with a peroxidase-based sensor. | a method for an enzymatic assay of histamine by using histamine oxidase from arthrobacter globiformis in combination with amperometric determination of h2o2 is described. histamine could be quantified at a level as low as 10(-7) m. the assay is adaptable to determine histamine in food samples including tuna fish with good sensitivity and selectivity. | 2000 | 11055403 |
| enhanced tolerance of transgenic brassica juncea to choline confirms successful expression of the bacterial coda gene. | brassica juncea cv. pusa jaikisan was transformed with the coda gene for choline oxidase from arthrobacter globiformis with an aim to introduce glycine betaine biosynthetic pathway, as it lacks any means to synthesize glycine betaine. western blot analysis revealed the presence of choline oxidase in the protein extract from the coda transgenic lines, demonstrating that the bacterial coda gene had been successfully transcribed and translated in transgenic lines. good activity of choline oxidase i ... | 2000 | 11074276 |
| degradation of substituted phenylurea herbicides by arthrobacter globiformis strain d47 and characterization of a plasmid-associated hydrolase gene, puha. | arthrobacter globiformis d47 was shown to degrade a range of substituted phenylurea herbicides in soil. this strain contained two plasmids of approximately 47 kb (phrim620) and 34 kb (phrim621). plasmid-curing experiments produced plasmid-free strains as well as strains containing either the 47- or the 34-kb plasmid. the strains were tested for their ability to degrade diuron, which demonstrated that the degradative genes were located on the 47-kb plasmid. studies on the growth of these strains ... | 2001 | 11319111 |
| development of a rhodococcus recombinant strain for degradation of products from anaerobic dechlorination of pcbs. | the gram-positive bacterium rhodococcus sp. strain rha1, naturally containing the biphenyl pathway, was electroporated with a broad host range plasmid containing the 4-chlorobenzoate (4-cba) degradation operon (fcb) isolated from arthrobacter globiformis strain kzt1. the recombinant strain grew in medium containing 4-cba and 4-chlorobiphenyl (4-cb) as the only source of carbon, with stoichiometric release of chloride and a molar growth yield on 4-cb that suggested utilization of both biphenyl ri ... | 2001 | 11349275 |
| organization of the genes involved in dimethylglycine and sarcosine degradation in arthrobacter spp.: implications for glycine betaine catabolism. | the nucleotide sequences of two cloned dna fragments containing the structural genes of heterotetrameric sarcosine oxidase (soxbdag) and dimethylglycine dehydrogenase (dmg) from arthrobater spp. 1-in and arthrobacter globiformis, respectively, have been determined. open reading frames were identified in the soxbdag operon corresponding to the four subunits of heterotetrameric sarcosine oxidase by comparison with the n-terminal amino-acid sequences and the subunit relative molecular masses of the ... | 2001 | 11422368 |
| construction, overexpression, and purification of arthrobacter globiformis amine oxidase-strep-tag ii fusion protein. | the copper-containing amine oxidase from arthrobacter globiformis has been expressed and purified as a fusion protein with a c-terminal strep-tag ii peptide. this tag facilitates the rapid purification of the enzyme on a large scale using the streptactin poros medium. for example, we have demonstrated that 50 mg of protein can be obtained in 2 days from 2 l of escherichia coli. the purified fusion protein displays turnover and spectroscopic properties that are essentially identical to those of t ... | 2001 | 11483008 |
| [dynamic of the volume and structure of the soil bacterial complex in the presence of azobenzene]. | azobenzene exerted no significant effect on the dynamics and the species composition of the saprophytic soil bacterial complex, which remained almost the same as in the control and was characterized by the predominance of curtobacterium sp., arthrobacter globiformis, and bacillus megaterium in all stages of succession. some heterotrophic bacteria were found to be able to accumulate azobenzene. bac. cereus and bac. polymyxa degraded azobenzene during their cultivation in nutrient media. | 2001 | 11558283 |
| spectroscopic observation of intermediates formed during the oxidative half-reaction of copper/topa quinone-containing phenylethylamine oxidase. | the catalytic reaction of copper/topa quinone (tpq) containing amine oxidase consists of the initial, well-characterized, reductive half-reaction and the following, less studied, oxidative half-reaction. we have analyzed the oxidative half-reaction catalyzed by phenylethylamine oxidase from arthrobacter globiformis (agao) by rapid-scan stopped-flow measurements. upon addition of dioxygen to the substrate-reduced agao at ph 8.2, the absorption bands derived from the semiquinone (tpq(sq)) and amin ... | 2001 | 11747456 |
| [synthesis of anabiosis autoinducers in non-spore-forming bacteria as a mechanism regulating their activity in soil and subsoil sedimentary rocks]. | non-spore-forming bacteria of the genera arthrobacter and micrococcus, isolated from permafrost subsoil, were found to produce greater amounts of the d1 extracellular factor than closely related collection strains isolated from soil. the effect of this factor, responsible for cell transition to anabiosis, was not species-specific. thus, the d1 crude preparation isolated from the culture liquid of the permafrost isolate arthrobacter globiformis 245 produced an effect on the collection strain arth ... | 2001 | 11763780 |
| rates of oxygen and hydrogen exchange as indicators of tpq cofactor orientation in amine oxidases. | this study presents the first detailed examination by resonance raman (rr) spectroscopy of the rates of solvent exchange for the c5 and c3 positions of the tpq cofactor in several wild-type copper-containing amine oxidases and mutants of the amine oxidase from hansenula polymorpha (hpao). on the basis of crystal structure analysis and differing rates of c5 [double bond] o and c3 [bond] h exchange within the enzyme systems, but equally rapid rates of c5 [double bond] o and c3 [bond] h exchange in ... | 2002 | 11781110 |
| inhibition of copper amine oxidases by pyridine-derived aldoximes and ketoximes. | the reactions of pea diamine oxidase (psao) and 2-phenylethylamine oxidase from arthrobacter globiformis (agao) with pyridine-derived oximes were studied. pyridine carbaldoximes and alkyl pyridyl ketoximes act as strong non-competitive inhibitors of the enzymes. the inhibition constants k(i) of these compounds vary between 10(-4) and 10(-5) m, for agao and some of the studied oximes were found even micromolar k(i) values. the presence of pyridine moiety in the studied compounds has remarkable in ... | 2001 | 11879727 |
| mechanistic aspects of the covalent flavoprotein dimethylglycine oxidase of arthrobacter globiformis studied by stopped-flow spectrophotometry. | dimethylglycine oxidase (dmgo) is a covalent flavoenzyme from arthrobacter globiformis that catalyzes the oxidative demethylation of dimethylglycine to yield sarcosine, formaldehyde, and hydrogen peroxide. stopped-flow and steady-state kinetic studies have been used to study the reductive and oxidative half-reactions using dimethylglycine and o2 as substrates. the reductive half-reaction is triphasic. the rate of the fast phase is dependent on substrate concentration, involves flavin reduction, ... | 2002 | 11926836 |
| [bacterial succession on n-alkanes under the conditions of sulfate reduction]. | the dynamics of species composition of a hydrocarbon-oxidizing bacteriocenosis of a ground suspension of mozhaisk reservoir has been studied. the bacteriocenosis was undergoing development in a paraffin film (model association composed of sulfate-reducing bacteria and hydrocarbon-oxidizing bacteria). the type of bacterial succession did not depend on the depth, from which ground samples were collected. two microbial species (pseudomonas sp. and arthrobacter globiformis) were absolutely dominant. ... | 2002 | 11962208 |
| [the role of low-molecular-weight nitrogen compounds in the osmotolerance of rhodococcus erythropolis and arthrobacter globiformis]. | investigations showed that rhodococcus erythropolis e-15 and arthrobacter globiformis 2f cells respond to osmotic shock by increasing the synthesis of free amino acids, primarily glutamic acid (80% of the intracellular free amino acid pool). the osmoprotective role of glutamic acid follows from its beneficial effect on the growth of bacteria in high-salinity media. it was found that the addition of this amino acid to the growth medium at a concentration of 2 mm shortened the lag phase and increa ... | 2002 | 12024813 |
| response of furniture factory workers to work-related airborne allergens. | the aim of this work was to determine the reactivity of furniture factory workers to microbial allergens associated with wood dust. allergological examinations by skin and precipitin tests were performed in 48 workers employed in a factory producing furniture from fibreboards and chipboards, and in 32 healthy urban dwellers not exposed to organic dusts (referents). the skin test was performed by the intradermal method with the saline extracts of the cultures of 3 microbial species (rahnella sp., ... | 2002 | 12088404 |
| x-ray snapshots of quinone cofactor biogenesis in bacterial copper amine oxidase. | the quinone cofactor tpq in copper amine oxidase is generated by posttranslational modification of an active site tyrosine residue. using x-ray crystallography, we have probed the copper-dependent autooxidation process of tpq in the enzyme from arthrobacter globiformis. apo enzyme crystals were anaerobically soaked with copper; the structure determined from this crystal provides a view of the initial state: the unmodified tyrosine coordinated to the bound copper. exposure of the copper-bound cry ... | 2002 | 12134140 |
| towards the development of selective amine oxidase inhibitors. mechanism-based inhibition of six copper containing amine oxidases. | four substrate analogs, 4-(2-naphthyloxy)-2-butyn-1-amine (1), 1,4-diamino-2-chloro-2-butene (2), 1,6-diamino-2,4-hexadiyne (3), and 2-chloro-5-phthalimidopentylamine (4) have been tested as inhibitors against mammalian, plant, bacterial, and fungal copper-containing amine oxidases: bovine plasma amine oxidase (bpao), equine plasma amine oxidase (epao), pea seedling amine oxidase (psao), arthrobacter globiformis amine oxidase (agao), escherichia coli amine oxidase (ecao), and pichia pastoris lys ... | 2002 | 12153561 |
| citricoccus muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting. | a gram-positive, aerobic, spherical actinobacterium, designated strain 4-0(t), was isolated from a medieval wall painting and characterized to determine its taxonomic position. the peptidoglycan of strain 4-0(t) was of type a4alpha, with lysine as the diagnostic cell wall diamino acid and an interpeptide bridge of lys-gly-glu. its quinone system contained predominantly mk-9(h2) (64%) and its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, four ... | 2002 | 12508874 |
| role of copper ion in bacterial copper amine oxidase: spectroscopic and crystallographic studies of metal-substituted enzymes. | the role of the active site cu(2+) of phenylethylamine oxidase from arthrobacter globiformis (agao) has been studied by substitution with other divalent cations, where we were able to remove >99.5% of cu(2+) from the active site. the enzymes reconstituted with co(2+) and ni(2+) (co- and ni-agao) exhibited 2.2 and 0.9% activities, respectively, of the original cu(2+)-enzyme (cu-agao), but their k(m) values for amine substrate and dioxygen were comparable. x-ray crystal structures of the co- and n ... | 2003 | 12537504 |
| transgenics of an elite indica rice variety pusa basmati 1 harbouring the coda gene are highly tolerant to salt stress. | transgenic lines of indica rice were generated by agrobacterium-mediated transformation with the choline oxidase ( coda) gene from arthrobacter globiformis. choline oxidase catalyses conversion of choline to glycine betaine. glycine betaine is known to provide tolerance against a variety of stresses. molecular analyses of seven independent transgenic lines as performed by southern, northern and western hybridization revealed integration and expression of the transgene as well as inheritance in t ... | 2002 | 12582870 |
| 4-nitrocatechol as a probe of a mn(ii)-dependent extradiol-cleaving catechol dioxygenase (mndd): comparison with relevant fe(ii) and mn(ii) model complexes. | mn(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase (mndd) is an extradiol-cleaving catechol dioxygenase from arthrobacter globiformis that has 82% sequence identity to and cleaves the same substrate (3,4-dihydroxyphenylacetic acid) as fe(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase (hpcd) from brevibacterium fuscum. we have observed that mndd binds the chromophoric 4-nitrocatechol (4-nch(2)) substrate as a dianion and cleaves it extremely slowly, in contrast to the fe(ii)-de ... | 2003 | 12589562 |
| kinetic mechanism of choline oxidase from arthrobacter globiformis. | choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. the enzyme is capable of accepting betaine-aldehyde as a substrate, allowing the investigation of the reaction mechanism for both the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. the steady state kinetic mechanism has been determined at ph 7 with choline and betaine-aldehyde ... | 2003 | 12637017 |
| inhibition of six copper-containing amine oxidases by the antidepressant drug tranylcypromine. | potential inhibitory effects of the clinically utilized monoamine oxidase inhibitor tranylcypromine (tcp) on mammalian, plant, bacterial, and fungal copper-containing amine oxidases have been examined. the following enzymes have been investigated: human kidney diamine oxidase (hkao), bovine plasma amine oxidase (bpao), equine plasma amine oxidase (epao), pea seedling amine oxidase (psao), arthrobacter globiformis amine oxidase (agao), and pichia pastoris lysyl oxidase (pplo). only bpao, epao, an ... | 2003 | 12686142 |
| [the 1(2)-dehydrogenation of steroid substrates by nocardioides simplex vkm ac-2033d]. | the bacterium formerly known as arthrobacter globiformis 193 has high 1(2)-dehydrogenase activity toward pharmaceutically important steroids, 9(11)-dehydrocortexolone in particular. the complex analysis of the morphostructural, physiological, biochemical, and phylogenetic properties of this bacterium allowed us to reclassify it into nocardioides simplex (n. simplex vkm ac-2033d). | 2003 | 12698789 |
| structural characterization and mapping of the covalently linked fad cofactor in choline oxidase from arthrobacter globiformis. | the flavoenzyme choline oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine. earlier studies have shown that the choline oxidase from arthrobacter globiformis contains fad covalently linked to a histidine residue. to identify the exact type of flavin binding, the fad-carrying amino acid residue was released by acid hydrolysis. the fluorescence excitation maxima of the isolated aminoacylriboflavin, showing a hypsochromic shift of the near-ultraviolet band relative to ribofl ... | 2003 | 12795615 |
| gold electrodes wired for coupling with the deeply buried active site of arthrobacter globiformis amine oxidase. | diethylaniline-terminated oligo(phenyl-ethynyl)-thiol (dea-ope-sh) wires on au-bead electrodes facilitate electron tunneling to and from the deeply buried topaquinone (tpq) cofactor in arthrobacter globiformis amine oxidase (agao). reversible cyclic voltammograms were observed when agao was adsorbed onto this dea-ope-sau surface: the 2e-/2h+ reduction potential is -140 mv versus sce. | 2003 | 12797771 |
| members of the genus arthrobacter grow anaerobically using nitrate ammonification and fermentative processes: anaerobic adaptation of aerobic bacteria abundant in soil. | members of the genus arthrobacter are usually regarded as obligate aerobic bacteria. the anaerobic growth and energy metabolism of two arthrobacter species were investigated. arthrobacter globiformis utilized both nitrate ammonification and lactate, acetate and ethanol producing fermentation processes for anaerobic growth. only nitrate supported anaerobic growth of arthrobacter nicotianae. anaerobically induced respiratory nitrate reductase activity was detected in both strains. neither of the t ... | 2003 | 12829291 |
| channelling and formation of 'active' formaldehyde in dimethylglycine oxidase. | here we report crystal structures of dimethylglycine oxidase (dmgo) from the bacterium arthrobacter globiformis, a bifunctional enzyme that catalyzes the oxidation of n,n-dimethyl glycine and the formation of 5,10-methylene tetrahydrofolate. the n-terminal region binds fad covalently and oxidizes dimethylglycine to a labile iminium intermediate. the c-terminal region binds tetrahydrofolate, comprises three domains arranged in a ring-like structure and is related to the t-protein of the glycine c ... | 2003 | 12912903 |
| growth interactions of arthrobacter globiformis and pseudomonas sp. in relation to the rhizosphere effect. | | 1961 | 13878001 |
| on the biotin requirement of arthrobacter globiformis. | | 1962 | 13878002 |
| glutamic acid production by arthrobacter globiformis. | | 1963 | 13996592 |
| production of a gibberellin-like substance by arthrobacter globiformis. | | 1962 | 14031348 |
| morphological aberration of arthrobacter globiformis cells due to biotin deficiency. | chan, e. c. s. (university of new brunswick, fredericton, new brunswick, canada). morphological aberration of arthrobacter globiformis cells due to biotin deficiency. j. bacteriol. 87:641-651. 1964.-morphological aberration of arthrobacter globiformis strain 425 was shown to occur during growth in a chemically defined medium without added biotin. such aberrant cells could revert back to normal coccoid forms upon inoculation into fresh medium supplemented with the vitamin. this abnormal cellular ... | 1964 | 14127583 |
| branching enzyme of arthrobacter globiformis. | | 1964 | 14171890 |
| [effect of the oxidation-reduction potential on the transformation of hydrocortisone by mycobacterium globiforme 193 cultures]. | | 1965 | 14286244 |
| studies of the lipids of arthrobacter globiformis 616. i. the fatty acid composition. | | 1965 | 14332529 |
| production of an egg yolk antibody specific to microbial uricase and its inhibitory effects on uricase activity. | ammonia gas produced from poultry manure can be a potential source of environmental pollution. microbial uricase in poultry manure is an important target enzyme to reduce ammonia production because ammonia is mainly generated from the microbial decomposition of uric acid in the manure. thus, the inhibition of microbial uricase is critical in preventing nh3 volatilization. a potential method of inhibiting uricase activity is the use of antibodies specific to microbial uricase. therefore, this stu ... | 2003 | 14601732 |
| expression, crystallization and preliminary x-ray crystallographic studies of arthrobacter globiformis inulin fructotransferase. | a recombinant form of arthrobacter globiformis inulin fructotransferase (dfaiii-producing) has been overexpressed in escherichia coli and purified to homogeneity. crystals were obtained at 293 k by the hanging-drop vapour-diffusion technique using 0.1 m na hepes ph 7.5 buffer containing 1.5 m lithium sulfate as a precipitant. crystals of the recombinant wild-type enzyme diffracted to better than 1.5 a at 100 k using a synchrotron-radiation source at the photon factory. the crystal belonged to sp ... | 2003 | 14646096 |
| structural insights into substrate specificity and function of glucodextranase. | a glucodextranase (igdase) from arthrobacter globiformis i42 hydrolyzes alpha-1,6-glucosidic linkages of dextran from the non-reducing end to produce beta-d-glucose via an inverting reaction mechanism and classified into the glycoside hydrolase family 15 (gh15). here we cloned the igdase gene and determined the crystal structures of igdase of the unliganded form and the complex with acarbose at 2.42-a resolution. the structure of igdase is composed of four domains n, a, b, and c. domain a forms ... | 2004 | 14660574 |
| cloning, sequence analysis, and purification of choline oxidase from arthrobacter globiformis: a bacterial enzyme involved in osmotic stress tolerance. | choline oxidase catalyzes the four-electron oxidation of choline to glycine betaine, one of a limited number of compounds that accumulate to high levels in the cytoplasm of cells to prevent dehydration and plasmolysis in adverse hyperosmotic environments. in the present study, the highly gc rich coda gene encoding for choline oxidase was cloned from genomic dna of arthrobacter globiformis strain atcc 8010 and expressed to high yields in escherichia coli strain rosetta(de3)plyss. the resulting en ... | 2004 | 14678796 |
| spectroscopic and kinetic properties of recombinant choline oxidase from arthrobacter globiformis. | choline oxidase catalyzes the four-electron oxidation of choline to glycine betaine, with molecular oxygen acting as primary electron acceptor. recently, the recombinant enzyme expressed in escherichia coli was purified to homogeneity and shown to contain fad in a mixture of oxidized and anionic semiquinone redox states [fan et al. (2003) arch. biochem. biophys., in press]. in this study, methods have been devised to convert the enzyme-bound flavin semiquinone to oxidized fad and vice versa, all ... | 2003 | 14690428 |
| stereochemistry of 2-phenylethylamine oxidation catalyzed by bacterial copper amine oxidase. | the stereochemical course of the reaction catalyzed by a copper amine oxidase from arthrobacter globiformis has been investigated using 2-phenylethylamine stereospecifically deuterium-labeled at the c1 position. measurements of deuterium content in the product, phenylacetaldehyde, by gas chromatography-mass spectrometry revealed stereospecific abstraction of the pro-s hydrogen during the enzymatic oxidation, as predicted from the structure modeling for the enzyme-bound substrate. | 2003 | 14730151 |
| chemical rescue of a site-specific mutant of bacterial copper amine oxidase for generation of the topa quinone cofactor. | the topa quinone (tpq) cofactor of copper amine oxidase is produced by posttranslational modification of a specific tyrosine residue through the copper-dependent, self-catalytic process. we have site-specifically mutated three histidine residues (his431, his433, and his592) involved in binding of the copper ion in the recombinant phenylethylamine oxidase from arthrobacter globiformis. the mutant enzymes, in which each histidine was replaced by alanine, were purified in the cu/tpq-free precursor ... | 2004 | 14979714 |
| cyanide as a copper and quinone-directed inhibitor of amine oxidases from pea seedlings ( pisum sativum) and arthrobacter globiformis: evidence for both copper coordination and cyanohydrin derivatization of the quinone cofactor. | the interactions of cyanide with two copper-containing amine oxidases (cuaos) from pea seedlings (psao) and the soil bacterium arthrobacter globiformis (agao) have been investigated by spectroscopic and kinetic techniques. previously, we rationalized the effects of azide and cyanide for several cuaos in terms of copper coordination by these exogenous ligands and their effects on the internal redox equilibrium tpq(amr)-cu(ii) right harpoon over left harpoon tpq(sq)-cu(i). the mechanism of cyanide ... | 2004 | 14986071 |
| crystallization and preliminary x-ray study of isomaltodextranase from arthrobacter globiformis. | a recombinant isomaltodextranase (1,6-alpha-d-glucan isomaltohydrolase; ec 3.2.1.94) from an arthrobacter sp. that hydrolyzes dextrans to generate isomaltose was purified and crystallized using the sitting-drop vapour-diffusion method at 293 k. x-ray diffraction data were collected to 1.8 a. the crystals belong to space group c2, with unit-cell parameters a = 199.1, b = 62.7, c = 57.4, beta = 101.4 degrees. analysis of the patterson self-rotation function suggests that the crystal contains one p ... | 2004 | 14993697 |
| crystallographic comparison of manganese- and iron-dependent homoprotocatechuate 2,3-dioxygenases. | the x-ray crystal structures of homoprotocatechuate 2,3-dioxygenases isolated from arthrobacter globiformis and brevibacterium fuscum have been determined to high resolution. these enzymes exhibit 83% sequence identity, yet their activities depend on different transition metals, mn2+ and fe2+, respectively. the structures allow the origins of metal ion selectivity and aspects of the molecular mechanism to be examined in detail. the homotetrameric enzymes belong to the type i family of extradiol ... | 2004 | 15028678 |
| casuarina cunninghamiana tissue extracts stimulate the growth of frankia and differentially alter the growth of other soil microorganisms. | aqueous extracts of host plant casuarina cunninghamiana tissue altered the in vitro growth of its diazotrophic microsymbiont frankia and a selection of other soil microorganisms. the growth of actinomycetous frankia strains, 55005. avci1, cesi5, cji82 001, and cj was stimulated by aqueous extracts of c. cunninghamiana tissue. green cladodes (photosynthetic branches), unsuberized roots, and suberized roots were more stimulatory than dry cladodes and seed tissue. aqueous extracts of green cladodes ... | 2004 | 15112734 |
| enzymatic synthesis of two novel non-reducing oligosaccharides using transfructosylation activity with beta-fructofuranosidase from arthrobacter globiformis. | two novel non-reducing oligosaccharides together with tri- and tetra-saccharides were synthesized by transfructosylation activity from sucrose as a donor and cellobiose or cellotriose as an acceptor with a purified beta-fructofuranosidase from arthrobacter globiformis ifo 3062, and these oligosaccharides were identified as o-beta-d-glucopyranosyl-(1-->4)-alpha-d-glucopyranosyl-(1-->2)-alpha,beta-d-fructofuranoside and o-beta-d-glucopyranosyl-(1-->4)-beta-d-glucopyranosyl-(1-->4)-alpha-d-glucopyr ... | 2004 | 15127791 |
| site-directed mutagenesis establishes aspartic acids-227 and -342 as essential for enzyme activity in an isomalto-dextranase from arthrobacter globiformis. | isomalto-dextranase, from arthrobacter globiformis t6, is a member of the glycoside hydrolase family 27. however, the alignments of the whole amino acid sequence are distinct from other members of this family. the enzymes cleave the glycosidic bond of the substrate in two different manners: either retaining or inverting the anomeric configuration. we believe that a retaining enzyme is involved in a two-step, double-displacement mechanism utilizing active site carboxylic acids as the nucleophile ... | 2004 | 15200177 |
| immunologic reactivity to work-related airborne allergens in people occupationally exposed to dust from herbs. | a group of 150 people occupationally exposed to dust from herbs were examined. the examined group consisted of 47 thyme farmers, 32 chamomile farmers, 31 sage farmers and 40 workers of herbs processing industry. as a reference group, 50 urban dwellers, not exposed to any kind of organic dust, were examined. skin prick tests and precipitin tests were conducted with, respectively, 4 and 11 microbial antigens associated with organic dust. both skin and precipitin tests were also conducted with herb ... | 2004 | 15236509 |
| hyper-production of an isomalto-dextranase of an arthrobacter sp. by a proteases-deficient bacillus subtilis: sequencing, properties, and crystallization of the recombinant enzyme. | arthrobacter globiformis t6 is unique in that it produces an enzyme yielding only isomaltose from dextran. in the present study, the organism was re-identified and its classification as a new species of the genus arthrobacter, a. dextranlyticum, was proposed. the high g+c gene (66.8 mol%) for the isomalto-dextranase was sequenced. the deduced amino acid sequence, with a calculated molecular mass of 65,993 da (603 amino acids), was confirmed by nanoscale capillary liquid chromatography coupled to ... | 2004 | 15248038 |
| [phylogenetic analysis of alpha-galactosidases of the gh27 family]. | amino acid sequence analysis of alpha-galactosidases and other proteins of glycoside hydrolase family 27 (gh27) allowed isolation of three major subfamilies, 27a-27c. unique isomalto-dextranase of arthrobacter globiformis clustered separately. eukaryotic proteins formed five clusters on a phylogenetic tree of the family. bacterial gh27 proteins, which are relatively few, did not form stable clusters. a monophyletic origin of the gh27 family was demonstrated with the use of related proteins of th ... | 2004 | 15285616 |
| differential inhibition of six copper amine oxidases by a family of 4-(aryloxy)-2-butynamines: evidence for a new mode of inactivation. | a series of compounds derived from a previously identified substrate analogue of copper amine oxidases (cuaos) (shepard et al. (2002) eur. j. biochem. 269, 3645-3658) has been screened against six different cuaos with a view to designing potent and selective inhibitors. the substrate analogues investigated were 4-(1-naphthyloxy)-2-butyn-1-amine, 4-(2-methylphenoxy)-2-butyn-1-amine, 4-(3-methylphenoxy)-2-butyn-1-amine, 4-(4-methylphenoxy)-2-butyn-1-amine, and 4-phenoxy-2-butyn-1-amine. these comp ... | 2004 | 15323556 |
| crystal structure of t-protein of the glycine cleavage system. cofactor binding, insights into h-protein recognition, and molecular basis for understanding nonketotic hyperglycinemia. | the glycine cleavage system catalyzes the oxidative decarboxylation of glycine in bacteria and in mitochondria of animals and plants. its deficiency in human causes nonketotic hyperglycinemia, an inborn error of glycine metabolism. t-protein, one of the four components of the glycine cleavage system,is a tetrahydrofolate dependent aminomethyltransferase. it catalyzes the transfer of the methylene carbon unit to tetrahydrofolate from the methylamine group covalently attached to the lipoamide arm ... | 2004 | 15355973 |
| genetic engineering of glycinebetaine synthesis in tomato protects seeds, plants, and flowers from chilling damage. | tomato (lycopersicon esculentum mill.) plants, which normally do not accumulate glycinebetaine (gb), are susceptible to chilling stress. exposure to temperatures below 10 degrees c causes various injuries and greatly decreases fruit set in most cultivars. we have transformed tomato (cv. moneymaker) with a chloroplast-targeted coda gene of arthrobacter globiformis, which encodes choline oxidase to catalyze the conversion of choline to gb. these transgenic plants express coda and synthesize cholin ... | 2004 | 15500464 |
| screening procedures for selecting rhizobacteria with biocontrol effects upon fusarium verticillioides growth and fumonisin b1 production. | screening is a critical step in the discovery of microbial agents that can exert biological control of fusarium verticillioides at the root level. the objectives of this research were to determine the utility of a niche overlap index to realise the first screening of maize rhizobacterial isolates during different water activities. studies were conducted to evaluate various methods for second screening with different modes of action. the antifungal activity of bacterial isolates through antibiosi ... | 2004 | 15501652 |
| [ultrastructure of resting cells of some non-spore-forming bacteria]. | using electron microscopy (ultrathin sections and freeze-fractures), we investigated the ultrastructure of the resting cells formed in the cultures of micrococcus luteus, arthrobacter globiformis, and pseudomonas aurantiaca under conditions of prolonged incubation (up to 9 months). these resting cells included cyst-like forms that were characterized by complex cell structure and the following ultrastructural properties: (i) a thickened or multiprofiled cell wall (cw), typically made up of a laye ... | 2004 | 15521179 |
| cyclic tetrasaccharide-synthesizing enzymes from arthrobacter globiformis a19. | a bacterial strain arthrobacter globiformis a19 producing cyclic tetrasaccharide (cts) was isolated from soil. the enzymes, 6-alpha-glucosyltransferase (6gt) and 3-alpha-isomaltosyltransferase (imt), involved in the synthesis of cts were purified to homogeneity. the molecular and enzymatic properties of imt from a. globiformis were similar to those of enzymes from bacillus globisporus c11 and n75. arthrobacter 6gt had a smaller molecular mass of 108 kda and a higher optimum ph of 8.4 than the en ... | 2004 | 15618624 |
| a strain of arthrobacter that tolerates high concentrations of nitrate. | a gram-positive strain identified as arthrobacter globiformis cect 4500, tolerant to up to 1 m nitrate, was isolated from the grounds of a munitions factory. under strict aerobic conditions, this bacterium used a wide variety of c-sources to obtain the energy required for growth, which took place when the nitrate concentration in the medium was below 150 mm. cells of this bacterium growing in the absence of nitrate were seen as individual cells or forming pairs, whereas cells grown in the presen ... | 1997 | 15765585 |
| rhizobacteria and their potential to control fusarium verticillioides: effect of maize bacterisation and inoculum density. | fusarium verticillioides is the most important seed transmitted pathogen that infects maize. it produces fumonisins, toxins that have potential toxicity for humans and animals. control of f. verticillioides colonisation and systemic contamination of maize has become a priority area in food safety research. the aims of this research were (1) to characterise the maize endorhizosphere and rhizoplane inhabitant bacteria and fusarium spp., (2) to select bacterial strains with impact on f. verticillio ... | 2005 | 15803383 |