| automated microbiological assay of thiamin in serum and red cells. | to develop a sensitive, direct, automated method for the measurement of serum and red cell thiamin. | 1994 | 8089221 |
| the aggregation of human platelets by lactobacillus species. | the ability to aggregate human platelets was examined for five lactobacillus rhamnosus strains and five lactobacillus paracasei subsp. paracasei strains isolated from patients with infective endocarditis (ie), 25 laboratory isolates from the same two species, and 14 strains from five other oral species, namely lactobacillus acidophilus, lactobacillus fermentum, lactobacillus oris, lactobacillus plantarum and lactobacillus salivarius. amongst the l. rhamnosus strains, platelets were aggregated by ... | 1993 | 8126421 |
| identification of lactobacilli from sourdough and description of lactobacillus pontis sp. nov. | the microflora of sourdough preparations was investigated by examining the physiological characteristics, whole-cell protein patterns, and 16s rrna sequences of lactobacillus isolates. strains isolated from sourdough were placed in the species lactobacillus brevis, lactobacillus sanfrancisco, and lactobacillus reuteri. 16s rrna sequences were determined for l. brevis, lactobacillus fructivorans, lactobacillus fermentum, l. sanfrancisco, and l. reuteri, and oligonucleotide probes for fast specifi ... | 1994 | 8186088 |
| thiamin status in pregnancy as determined by direct microbiological assay. | serum and red cell thiamin levels were assayed on a group of pregnant australian women using an automated direct microbiological assay with lactobacillus fermenti as the test organism. two of 103 (1.9%) had serum thiamin levels below the reference range and 2 of 67 (3.0%) were found to have red cell thiamin levels below the reference range. although the mean serum levels between trimesters were not significantly different, there was a significant decrease in the mean red cell thiamin in the thir ... | 1994 | 8200746 |
| lactobacillus cells in the rabbit digestive tract and the factors affecting their distribution. | lactobacillus fermentum was present in small numbers in the caecum and colon of only one of 16 rabbits. studies aimed at elucidating the factors affecting the colonization of lactobacilli in rabbit gut were thus performed. these studies included investigation of the effect of gastric juice and bile salts on the viability of lactobacilli, assay of the survival rates of lactobacilli in the gastrointestinal tracts of the rabbits with or without ileum cannulation and measurement of the adhesive capa ... | 1993 | 8244905 |
| coaggregation of oral lactobacilli with streptococci from the oral cavity. | the ability of oral lactobacilli to coaggregate with streptococci and actinomycetes was investigated. of the 7 species of lactobacilli studied, only two were capable of coaggregation and the coaggregation was restricted to streptococci. lactobacillus salivarius strains (2/4) coaggregated with streptococcus salivarius, streptococcus gordonii, streptococcus crista and tufted streptococcus sanguis ii strains. lactobacillus fermentum (2/3) coaggregated with s. gordonii and s. sanguis. the coaggregat ... | 1993 | 8265207 |
| [studies on the locality and the function of spirosome in bacterial cells: relationship between the production of spirosome and anaerobic glycolysis]. | the locality and the function of spirosome were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and electron microscopy in peptostreptococcus productus, clostridium sp. hd-17, lactobacillus fermentum, eubacterium aerofaciens and escherichia cali b. when the bacterial cells in phosphate buffered saline were disrupted by sonication and fractionated by differential centrifugation, the spirosome protein was found in the cytoplasmic fraction and not in the fraction of ... | 1993 | 8315808 |
| purification, cloning, and cofactor independence of glutamate racemase from lactobacillus. | glutamate racemase has been purified more than 12,000-fold from lactobacillus fermenti. the racemase gene has been cloned using standard hybridization techniques combined with a novel selection for in vivo glutamate racemase activity, and the racemase has been expressed in escherichia coli as 20-25% of the total soluble cell protein. the cloned gene product is indistinguishable from that purified from lactobacillus and is a monomer of m(r) 28,300. both a coupled enzymatic assay and a circular di ... | 1993 | 8385993 |
| the binding of pneumococcal lipoteichoic acid to human erythrocytes. | previous studies have evaluated the binding characteristics of lipoteichoic acids (lta) composed of polyglycerol phosphate to mammalian cell membranes. this present study was performed to evaluate the binding characteristics of pneumococcal lta, a polyribitol phosphate containing lta, to human erythrocytes. binding was found to be specific, reversible, and dependent on temperature and time. maximum binding was achieved within 2 h at 37 degrees c. scatchard plot analysis of the binding revealed a ... | 1993 | 8391621 |
| inhibition of adhesion of escherichia coli k88 to piglet ileal mucus by lactobacillus spp. | enteropathogenic escherichia coli k88 colonizing the piglet ileum adhere to the mucosa by k88 fimbrial appendages. a recent study in our laboratory has implicated indigenous lactobacilli in the suppression of the colonization potential of enteropathogenic e. coli as measured by adhesion to ileal mucus. the aim of this study was to investigate the effect of lactobacillus spp. of porcine origin on the adhesion of k88 fimbriae of e. coli. with an in vitro assay, the adhesion of e. coli k88ab strain ... | 1993 | 8439162 |
| the antimicrobial activity of lactic acid bacteria from fermented maize (kenkey) and their interactions during fermentation. | a total of 241 lactic acid bacteria belonging to lactobacillus plantarum, pediococcus pentosaceus, lactobacillus fermentum/reuteri and lactobacillus brevis from various processing stages of maize dough fermentation were investigated. results indicated that each processing stage has its own microenvironment with strong antimicrobial activity. about half of the lact. plantarum and practically all of the lact. fermentum/reuteri investigated were shown to inhibit other gram-positive and gram-negativ ... | 1995 | 8567490 |
| adhesion of lactobacillus fermentum 104-s to porcine stomach mucus. | the adhesion to whole and fractionated porcine gastric mucus of both lactobacillus fermentum 104-s cells and a saccharide extracted from this strain was investigated. it has been shown previously that this saccharide had affinity for nonsecreting gastric epithelium. the mucus component(s) with affinity the bacterial cells was partly characterized by gel filtration and treatment with protease or metaperiodate. l. fermentum 104-s extracts containing the saccharide were radioactively labeled, fract ... | 1996 | 8661685 |
| the potential of lactobacillus as a carrier for oral immunization: development and preliminary characterization of vector systems for targeted delivery of antigens. | oral administration of lactobacilli evokes mucosal and systemic immune responses against epitopes associated with these organisms (gerritse et al., 1990, 1991). the adjuvant function of different lactobacillus species was investigated under the conditions of intraperitoneal (i.p.) injection or oral administration. after i.p. injection of trinitrophenylated chicken gamma-globulin, high dth responses were observed with lactobacillus casei and lactobacillus plantarum, but low responses with lactoba ... | 1996 | 8717402 |
| effect of adherent lactobacillus spp. on in vitro adherence of salmonellae to the intestinal epithelial cells of chicken. | single strains of lactobacillus acidophilus and lact. fermentum, isolated from chicken intestine, were used to study in vitro interactions with salmonella enteritidis, salm. pullorum or salm. typhimurium in an ileal epithelial cell (iec) radioactive assay. exclusion, competition and displacement phenomena were investigated by respectively incubating (a) lactobacilli and iec together, prior to addition of salmonellae, (b) lactobacilli, iec and salmonellae together, and (c) salmonellae and iec, fo ... | 1996 | 8760330 |
| inhibition of initial adhesion of uropathogenic enterococcus faecalis by biosurfactants from lactobacillus isolates. | in this study, 15 lactobacillus isolates were found to produce biosurfactants in the mid-exponential and stationary growth phases. the stationary-phase biosurfactants from lactobacillus casei subsp. rhamnosus 36 and atcc 7469, lactobacillus fermentum b54, and lactobacillus acidophilus rc14 were investigated further to determine their capacity to inhibit the initial adhesion of uropathogenic enterococcus faecalis 1131 to glass in a parallel-plate flow chamber. the initial deposition rate of e. fa ... | 1996 | 8787394 |
| effect of lactobacilli and antibiotics on e. coli urinary infections in mice. | urinary antibiotic treatments usually affect the normal urethral flora. this work was developed in order to evaluate whether lactobacillus fermentum crl 1058 could reduce urinary tract infections (uti) produced by uropathogenic escherichia coli in mice treated with antimicrobial agents. animals were inoculated intra-urethrically with agarose beads containing lactobacilli, and were challenged with e. coli. ampicillin (13 mg/kg/dose) was administered orally. the number of microorganisms present at ... | 1996 | 8820918 |
| purification and characterization of a component produced by lactobacillus fermentum that inhibits the adhesion of k88 expressing escherichia coli to porcine ileal mucus. | it has previously been shown that lactobacillus fermentum strain 104r releases compounds into its culture fluid that inhibit the adhesion of enterotoxigenic escherichia coli k88. the aim of the present study was to purify and identify this compound. judged by gel filtration, the compound was found to be approximately 1700 kda. the amount of active compound increased upon prolonged incubation, while the number of viable cells reduced, suggesting that the activity was coming from dead cells. as th ... | 1996 | 8852678 |
| influence of carbohydrates on the alpha-galactosidase activity of lactobacillus fermentum | the influence of soybean galactosaccharides (stachyose, raffinose, melibiose) as well as galactose and glucose on the synthesis and activity of alpha-galactosidase (alpha-gal) from lactobacillus fermentum crl 251 was studied. stachyose was the most effective inducer, followed by melibiose, raffinose, and galactose; scarce activity was detected with glucose. exogenously supplied glucose inhibited the synthesis of the enzyme in cultures of l. fermentum growing on galactose. this effect was reverse ... | 1996 | 8875910 |
| a bifunctional urease enhances survival of pathogenic yersinia enterocolitica and morganella morganii at low ph. | to infect a susceptible host, the gastrointestinal pathogen yersinia enterocolitica must survive passage through the acid environment of the stomach. in this study, we showed that y. enterocolitica serotype o8 survives buffered acidic conditions as low as ph 1.5 for long periods of time provided urea is available. acid tolerance required an unusual cytoplasmically located urease that was activated 780-fold by low-ph conditions. acid tolerance of helicobacter species has also been attributed to u ... | 1996 | 8932305 |
| colonization by lactobacilli of piglet small intestinal mucus. | the colonization potential of lactobacilli was investigated using small intestinal mucus extracts from 35-d-old pigs. mucus-secreting tissue from the small intestine of piglets was gently rinsed to remove contents and then shaken in buffer to release mucus from the surface. numbers of lactobacilli in different portions of the small intestine of 35-d-old pigs were enumerated. also, mucus isolated from the small intestine of pigs was investigated for its capacity to support the growth of lactobaci ... | 1996 | 8939025 |
| use of sulfite and hydrogen peroxide to control bacterial contamination in ethanol fermentation. | lactic acid bacteria isolated from an industrial-scale ethanol fermentation process were used to evaluate sulfite as a bacterial-contamination control agent in a cell-recycled continuous ethanol fermentation process. the viabilities of bacteria were decreased by sulfite at concentrations of 100 to 400 mg liter-1, while sulfite at the same concentrations did not change the viability of the saccharomyces cerevisiae strain used in this process. sulfite was effective only in the presence of oxygen. ... | 1997 | 8979332 |
| nucleotide sequence of the bacillus licheniformis atcc 10716 dat gene and comparison of the predicted amino acid sequence with those of other bacterial species. | the gene encoding the d-aminotransferase from bacillus licheniformis was cloned and the complete dna sequence was determined. the deduced d-aminotransferase protein sequence, consists of 283 amino acids and shows a high degree of homology with other bacillus d-aminotransferases, branched chain aminotransferase of escherichia coli and the 4-amino-benzoate-4-deoxychorismate lyase of bacillus subtilis and escherichia coli. | 1997 | 9003455 |
| [the antagonistic properties of microaerophilic bacteria isolated from the human and mink digestive tracts]. | study of antagonistic properties of microaerophilic bacteria isolated from human and mink gastroenteric tract have helped to establish differences in species composition, quantity and level of antagonistic activity of the studied microorganisms in respect to pathogenic microflora. it is shown that lactic acid bacteria identified as lactobacillus fermentum and l. reuteri prevail among the strains isolated from the stomach and thin intestine of minks kept in the 30-km zone of chernobyl npp. specie ... | 1996 | 9044713 |
| probiotic treatment of small intestinal bacterial overgrowth by lactobacillus fermentum kld. | the principle of using harmless bacteria for conquering pathogens has been used for many years. it has been used prophylactically against travellers' diarrhoea and for protection of recurrent pseudomembranous colitis. the aim of this study was to treat a chronic infectious condition, small intestinal bacterial overgrowth, by oral administration of a certain strain of lactobacillus. 17 patients with long-standing bacterial overgrowth of the small intestine were included. the study was designed as ... | 1996 | 9060066 |
| microflora of boza, a traditional fermented turkish beverage. | boza, a turkish traditional beverage made by yeast and lactic acid fermentation of cooked maize, wheat and rice flours was prepared and the microbial characteristics were investigated. during the course of fermentation from 0 to 24 h, populations of lactic acid bacteria and yeast raised from 7.6 x 10(6) and 2.25 x 10(5) after inoculation to 4.6 x 10(8) and 8.1 x 10(6), respectively; ph dropped from 6.1 to 3.5; total titratable acidity by means of lactic acid increased from 0.02 to 0.27 mmol/g; a ... | 1997 | 9105937 |
| cell wall anchoring of the streptococcus pyogenes m6 protein in various lactic acid bacteria. | the m6 protein from streptococcus pyogenes is the best-characterized member of a family of cell envelope-associated proteins. based on the observation that the c-terminal sorting signals of these proteins can drive cell wall anchoring of heterologous unanchored proteins, we have cloned and expressed the emm6 structural gene for the m6 protein in various lactic acid bacteria (lab). the emm6 gene was successfully expressed from lactococcal promoters in several lactococcus lactis strains, an animal ... | 1997 | 9139932 |
| identification and characterization of a basic cell surface-located protein from lactobacillus fermentum br11. | extraction of lactobacillus fermentum br11 cells with 5 m licl yielded a preparation containing a single predominant polypeptide with an apparent molecular mass of 32 kda. a clone encoding an immunoreactive 32-kda polypeptide was isolated from a puc18 library of l. fermentum br11 dna by screening with an antiserum raised against whole cells of l. fermentum br11. sequence determination of the insert in the clone revealed a complete 795-bp open reading frame (orf) that defines a 28,625-da polypept ... | 1997 | 9150229 |
| role of bacteria in the pathogenesis of short bowel syndrome-associated d-lactic acidemia. | previously, we have demonstrated that short bowel syndrome (sbs) patients suffer daily from d-lactic acidemia; in these patients rather high amounts of (bacterial) d-lactate emerge in blood and urine with a circadian rhythm. the aim of this study was to establish the microbial basis of d-lactic acidemia in sbs. therefore, faecal flora of (young and adult) sbs-patients was analysed qualitatively and quantitatively, and compared to that of controls. the isolated bacterial species were screened for ... | 1997 | 9160298 |
| isolation and characterization of a plasmid from lactobacillus fermentum conferring erythromycin resistance. | lactobacillus fermentum is a lactic acid bacterial species commonly found in the digestive tracts of pigs and rodents and also present in man. we characterized a 5.7-kb plasmid, plem3, conferring erythromycin resistance, which was isolated from a porcine strain of l. fermentum. plasmid plem3 established efficiently in l. fermentum, conferred high-level erythromycin resistance (mic > 1 mg/ml), and was segregationally stable. a deletion derivative of plem3, called plem5, was constructed and found ... | 1997 | 9200223 |
| digoxigenin-labeled deoxyribonucleic acid probes for the enumeration of bifidobacteria in fecal samples. | the numbers of bifidobacteria in fecal samples were specifically determined by colony hybridization with the mixture of digoxigenin-labeled dna probes that were prepared from whole chromosomal dna of bifidobacterium longum 6001 and bifidobacterium adolescentis 6003. these dna probes strongly hybridized with dna of b. longum, b. adolescentis, bifidobacterium breve, bifidobacterium suis, bifidobacterium infantis, bifidobacterium bifidum, bifidobacterium angulatum, and bifidobacterium animalis. det ... | 1997 | 9241587 |
| identification and phylogenetic analysis of lactobacillus using multiplex rapd-pcr. | multiplex rapd-pcr was used to generate unique and identifying dna profiles for isolates of the genus lactobacillus. the method that was used was based on the combination of two 10-mer oligonucleotides in a single pcr. the generated rapd profiles enabled discrimination of all lactobacillus strains that were used in this study. a dendrogram was generated from the rapd profiles. the results of genetic relatedness obtained from the dendrogram were compared with the results obtained using carbohydra ... | 1997 | 9252586 |
| feeding resistant starch affects fecal and cecal microflora and short-chain fatty acids in rats. | the effects of different forms of resistant potato starch (rs) on the major microbial population groups and short-chain fatty acids (scfa) in the cecum and feces of rats were studied over a 5-mo feeding period. thirty 8-wk-old male wistar rats, averaging 210 g initial body weight, were adapted for 7 d to a balanced basal diet containing 60% waxy maize starch devoid of any rs. on d 8, three groups of 10 rats each were fed diets containing the following forms of starch: 1) rapidly digestible waxy ... | 1997 | 9303464 |
| effects of lactobacilli on yeast-catalyzed ethanol fermentations. | normal-gravity (22 to 24 degrees plato) wheat mashes were inoculated with five industrially important strains of lactobacilli at approximately 10(5), approximately 10(6), approximately 10(7), approximately 10(8), and approximately 10(9) cfu/ml in order to study the effects of the lactobacilli on yeast growth and ethanol productivity. lactobacillus plantarum, lactobacillus paracasei, lactobacillus #3, lactobacillus rhamnosus, and lactobacillus fermentum were used. controls with yeast cells but no ... | 1997 | 9361399 |
| synthesis of nitric oxide from the two equivalent guanidino nitrogens of l-arginine by lactobacillus fermentum. | ten strains of lactobacillus fermentum that differed in origin converted metmyoglobin to nitrosylmyoglobin [a pentacoordinate nitric oxide (no) complex of fe(ii) myoglobin] in mrs broth at ph 4.3. of the 10 strains, l. fermentum ifo 3956 possessed the strongest capacity to convert metmyoglobin to nitrosylmyoglobin. this strain synthesizes no enzymatically from the two equivalent guanidino nitrogens of l-arginine. to our knowledge, this demonstrates for the first time the production of no synthes ... | 1997 | 9401042 |
| characterization of the bacterial flora of sudanese sorghum flour and sorghum sourdough. | the microflora of a sudanese sorghum flour, a spontaneously fermented sourdough and a long-term sourdough produced in a sudanese household by consecutive re-inoculations, was studied. the dominant contaminants of sorghum flour were gram-negative, catalase-positive, rod-shaped bacteria with counts of about 10(5) cfu g-1. the spontaneously fermented sorghum sourdough showed a bacterial succession from gram-negative, catalase-positive contaminants to enterococcus faecalis, lactococcus lactis, lacto ... | 1997 | 9449814 |
| primary peritonitis due to lactobacillus fermentum. | | 1998 | 9570668 |
| lactobacillus species associated with active caries lesions. | lactobacilli that are most frequently associated with active caries lesions in dentine were isolated and identified as part of a research project which aims to determine the role of the predominant species of these organisms in the carious process. samples of carious dentine were collected from 12 patients with open caries lesions (group a) and stimulated saliva samples were collected from 12 patients with a dmft = 0 and confirmed presence of lactobacilli in the oral cavity (group b). after seri ... | 1998 | 9588995 |
| colonization capability of orally administered lactobacillus strains in the gut of gnotobiotic piglets. | in the present study, the effect of lactobacillus casei subsp. casei and lactobacillus fermentum inoculation on jejunum and ileum colonization in gnotobiotic piglets has been observed. the characteristic features of the strains used were strong adherence to pig epithelial gut cells as well as inhibitory activity against enteropathogenic e. coli under in vitro conditions. strains were inoculated to 2, 3, and 4 day old gnotobiotic piglets at a dose of 2 ml (1 x 10(8) germs/ml). on the second day a ... | 1998 | 9646555 |
| development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic lactobacillus and bifidobacterium species in the upper human gastrointestinal tract. | an in vitro methodology which mimics in vivo human upper gastrointestinal transit was developed. the transit tolerance of potentially probiotic lactobacillus and bifidobacterium species was determined by exposing washed cell suspensions at 37 degrees c to a simulated gastric juice (ph 2.0), containing pepsin (0.3% w/v) and sodium chloride (0.5% w/v), and a simulated small intestinal juice (ph 8.0), containing pancreatin usp (1 g l-1) and sodium chloride (5 g l-1), and monitoring changes in total ... | 1998 | 9674129 |
| lactobacilli counts in crop, ileum and caecum of growing broiler chickens fed on practical diets containing whole or dehulled sweet lupin (lupinus angustifolius) seed meal. | 1. four experiments with growing broiler chickens were carried out to study the effects of the inclusion in their diets of lupin (lupinus angustifolius) seed meal of e. coli and lactobacilli counts in crop, ileum and caecca at 3 or 4 weeks of age. 2. diets were formulated to contain the same amounts of metabolisable energy (12.55 mj/kg) and protein (210 g/kg). raw whole (heat-untreated) or dehulled sweet (low in alkaloids) lupin seed meal (400 and 320 g/kg respectively) were used to prepare the ... | 1998 | 9693815 |
| purification and characterization of cystathionine gamma-lyase from lactobacillus fermentum dt41. | a homo-tetrameric ca. 140-kda cystathionine gamma-lyase was purified to homogeneity from lactobacillus fermentum dt41 by four chromatographic steps. this was the first enzyme responsible for amino acid catabolism purified from lactobacilli. the activity is pyridoxal-5'-phosphate dependent and the enzyme catalyzes the alpha,gamma-elimination reaction of l-cystathionine producing l-cysteine, ammonia and alpha-ketobutyrate. the cystathionine gamma-lyase produced a free thiol group, a keto acid comp ... | 1998 | 9770274 |
| microbial populations associated with the retting of melon pods (colocynthis citrullus l.) during seed recovery. | the traditional process for the retting of melon pulp and microbiological characteristics in the recovery of melon seeds (colocynthis citrullus l.) were investigated. melon pods were sliced open and exposed for seven days. the pulp underwent a natural fermentation that was characterized by the growth of microorganisms to 10(8)-10(10) cfu/g. the ph fluctuated between 4.8 and 5.1 with a lactic acid content of 0.72%. bacillus subtilis, b. polymyxa, lactobacillus fermentum, l. brevis and streptococc ... | 1998 | 9839833 |
| identification of lactic acid bacteria from chili bo, a malaysian food ingredient. | ninety-two strains of lactic acid bacteria (lab) were isolated from a malaysian food ingredient, chili bo, stored for up to 25 days at 28 degreesc with no benzoic acid (product a) or with 7,000 mg of benzoic acid kg-1 (product b). the strains were divided into eight groups by traditional phenotypic tests. a total of 43 strains were selected for comparison of their sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) whole-cell protein patterns with a sds-page database of lab. iso ... | 1999 | 9925588 |
| lectin-like protein fractions in lactic acid bacteria isolated from chickens. | agglutination of fifty bacterial strains isolated from gastric-intestinal tract of chickens with yeasts treated with glutaraldehyde and glycine was assayed to study possible adhesion mechanisms to epithelia. it was found that lactobacillus fermentum, lactobacillus fermentum subsp. cellobiosus and lactobacillus animalis agglutinated yeasts massively and this agglutination was inhibited in each strain by different representative sugars: agglutination was inhibited in three selected strains with 0. ... | 1999 | 9989654 |
| lactobacilli isolated from chicken intestines: potential use as probiotics. | lactobacillus strains were tested for their in vitro probiotic properties. cell surface hydrophobicity was found to be very high for lactobacillus fermentum subsp. cellobiosus and salmonella gallinarum; high values could indicate a greater ability to adhere to epithelial cells. studies on lactobacillus animalis indicated relative cell surface hydrophobicities smaller than those of l. fermentum subsp. cellobiosus and l. fermentum. l. animalis and enterococcus faecalis were able to coaggregate wit ... | 1999 | 10090244 |
| the bspa locus of lactobacillus fermentum br11 encodes an l-cystine uptake system. | bspa is a basic surface-exposed protein from lactobacillus fermentum br11. sequence comparisons have shown that it is a member of family iii of the solute binding proteins. it is 89% identical to the collagen binding protein, cnb, from lactobacillus reuteri. compared with the database of escherichia coli proteins, bspa is most similar to the l-cystine binding protein fliy. to investigate the function of bspa, mutants depleted for bspa were generated by homologous recombination with a temperature ... | 1999 | 10094698 |
| [grounds for use of lactobacterin from lactobacillus fermentum 90-ts-4 in gynecological practice]. | | 1999 | 10095966 |
| use of conserved randomly amplified polymorphic dna (rapd) fragments and rapd pattern for characterization of lactobacillus fermentum in ghanaian fermented maize dough. | the present work describes the use of randomly amplified polymorphic dna (rapd) for the characterization of 172 dominant lactobacillus isolates from present and previous studies of ghanaian maize fermentation. heterofermentative lactobacilli dominate the fermentation flora, since approximately 85% of the isolates belong to this group. cluster analysis of the rapd profiles obtained showed the presence of two main clusters. cluster 1 included lactobacillus fermentum, whereas cluster 2 comprised th ... | 1999 | 10388723 |
| starter culture activity in refrigerated fermented soymilk. | the refrigerated shelf life of soymilk fermented with single cultures of lactobacillus fermentum, l. casei, streptococcus salivarius subsp. thermophilus, and bifidobacterium longum was evaluated. during storage at 4 degrees c for 28 days, the stability of the microflora differed markedly among the starter cultures. after 28 days, the average numbers of s. salivarius subsp. thermophilus decreased by two log cycles to 6.0 x 10(7) cfu/ml, whereas those of l. casei increased gradually by more than t ... | 1999 | 10419278 |
| identification of and hydrogen peroxide production by fecal and vaginal lactobacilli isolated from japanese women and newborn infants. | we identified lactobacillus isolates from japanese women and newborn infants by a dna-dna hybridization method. the predominating lactobacilli were lactobacillus crispatus and lactobacillus gasseri in the women's vaginas and the newborns' intestines and l. gasseri and lactobacillus fermentum in the women's intestines. all l. crispatus strains were exclusively strong h2o2 producers. | 1999 | 10449509 |
| the broad host range plasmid plf1311 from lactobacillus fermentum vkm1311. | the complete nucleotide sequence (2389 bp) of the cryptic plasmid plf1311 from lactobacillus fermentum vkm1311 was determined. dna sequence analysis revealed the putative coding regions for a replicative protein (repb), its repressor (repa) and double-stranded (dso) and single-stranded (sso) origins. plf1311 belongs to the pe194 family of rolling circle-replicating plasmids. a derivative of plf1311 that contains the cat gene of plasmid pc194 of staphylococcus aureus and the orit of rp4 was const ... | 1999 | 10483722 |
| collagen (cn-i) binding by gut lactobacilli. | four gut lactobacillus strains displaying the features which make them particularly promising for the preparation of probiotic products were investigated together with 5 fresh isolates and one collection strain of lactobacillus plantarum for their ability to bind type i collagen (cn-i). immobilised cn-i in microtitre plates was bound only by 3 strains of gut lactobacilli from piglets and the collection strain lactobacillus plantarum lhi 10 from prague in range of a570nm readings 0.114-0.221. six ... | 1999 | 10486988 |
| expression of chlamydia psittaci- and human immunodeficiency virus-derived antigens on the cell surface of lactobacillus fermentum br11 as fusions to bspa. | the basic surface protein, bspa, has been used as a fusion partner to direct peptide antigens from the human immunodeficiency virus gp41 protein and the chlamydia psittaci ompa protein to the cell surface of lactobacillus fermentum br11. bspa has potential utility in the construction of live vaccines and diagnostic reagents. | 1999 | 10496936 |
| efficiency of transcription from promoter sequence variants in lactobacillus is both strain and context dependent. | the introduction of consensus -35 (ttgaca) and -10 (tataat) hexamers and a tg motif into the lactobacillus acidophilus atcc 4356 wild-type slpa promoter resulted in significant improvements (4.3-, 4.1-, and 10.7-fold, respectively) in transcriptional activity in lactobacillus fermentum br11. in contrast, the same changes resulted in decreased transcription in lactobacillus rhamnosus gg. the tg motif was shown to be important in the context of weak -35 and -10 hexamers (l. fermentum br11) or a co ... | 1999 | 10515955 |
| human cytokine responses induced by gram-positive cell walls of normal intestinal microbiota. | the normal microbiota plays an important role in the health of the host, but little is known of how the human immune system recognizes and responds to gram-positive indigenous bacteria. we have investigated cytokine responses of peripheral blood mononuclear cells (pbmc) to gram-positive cell walls (cw) derived from four common intestinal indigenous bacteria, eubacterium aerofaciens (eu.a. ), eubacterium limosum (eu.l.), lactobacillus casei (l.c.), and lactobacillus fermentum (l.f.). our results ... | 1999 | 10540188 |
| polyphasic study of the spatial distribution of microorganisms in mexican pozol, a fermented maize dough, demonstrates the need for cultivation-independent methods to investigate traditional fermentations. | the distribution of microorganisms in pozol balls, a fermented maize dough, was investigated by a polyphasic approach in which we used both culture-dependent and culture-independent methods, including microbial enumeration, fermentation product analysis, quantification of microbial taxa with 16s rrna-targeted oligonucleotide probes, determination of microbial fingerprints by denaturing gradient gel electrophoresis (dgge), and 16s ribosomal dna gene sequencing. our results demonstrate that dgge f ... | 1999 | 10584005 |
| immune response to orally consumed antigens and probiotic bacteria. | the gut mucosal system must fulfil conflicting roles in suppressing immune responses against orally fed antigens (tolerance) while still retaining the ability to respond to potential enteric pathogens. it must also, to a large degree, not mount an immune response against commensal enteric bacteria and the administration of large numbers of probiotic bacteria formulated as dietary supplements in food products. contrary to this dogma, it has been found that feeding ovalbumin as a marker antigen, i ... | 2000 | 10651930 |
| modulating immune responses with probiotic bacteria. | for many years, probiotic bacteria have been known to confer health benefits to the consumer. one possible mechanism for this may be the ability of probiotic bacteria to modulate immune responses. oral administration of lactobacillus casei strain shirota (lcs) has been found to enhance innate immunity by stimulating the activity of splenic nk cells. oral feeding with killed lcs was able to stimulate the production of th1 cytokines, resulting in repressed production of ige antibodies against oval ... | 2000 | 10651931 |
| lactobacillus flora in the cloaca and vagina of hens and its inhibitory activity against salmonella enteritidis in vitro. | lactobacilli in the cloaca and vagina of 40 normal laying hens were investigated quantitatively and qualitatively, and their ability to inhibit growth of salmonella enteritidis (se) was examined using a spot-the-lawn technique. all samples of cloacal contents and half the samples of vaginal mucus were positive for lactobacilli. the means +/- sd of total lactobacillus counts in the cloaca and those in the vagina were log10 5.5 +/- 1.1 and 2.5 +/- 2.6 cfu/g, respectively. in the cloaca, lactobacil ... | 2000 | 10685882 |
| some probiotic properties of chicken lactobacilli. | the beneficial effect of lactobacilli has been attributed to their ability to colonize human and animal gastrointestinal tracts. in this work, adhesion assays with three lactobacillus strains and intestinal fragments obtained from chickens were assessed. lactobacillus animalis and l. fermentum were able to adhere to three kinds of epithelial cells (crop, small and large intestines) with predominance to small intestine. among the strains considered, l. fermentum subsp. cellobiosus showed the lowe ... | 1999 | 10696476 |
| expression of bacillar glutamyl endopeptidase genes in bacillus subtilis by a new mobilizable single-replicon vector plf. | the plf1311 natural plasmid from lactobacillus fermentum 1311 was used to construct a single-replicon vector suitable for rapid cloning in a wide range of gram-positive hosts and escherichia coli. the new vector is capable of conjugative mobilization from e. coli to various hosts by conjugal transfer. the final vector (3.4 kb) showed a high segregational and structural stability and a high copy number. glutamyl endopeptidase genes from bacillus licheniformis (gsebl) and b. intermedius (gsebi) we ... | 2000 | 10783297 |
| [a family of shuttle vectors for lactobacilli and other gram-positive bacteria based on the plasmid plf1311 replicon]. | a set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in gram-positive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc.) was created. the vectors are based on the cryptic plasmid plf1311 from lactobacillus fermentum vkm 1311 belonging to a family of the sigma-type pe194-like plasmids. the vectors can replicate in gram-positive bacteria and escherichia coli. they are stable in many gram-positive bacteria, have small sizes, and allow the selection ... | 2000 | 10808493 |
| bacterial cell wall-induced arthritis: chemical composition and tissue distribution of four lactobacillus strains. | to study what determines the arthritogenicity of bacterial cell walls, cell wall-induced arthritis in the rat was applied, using four strains of lactobacillus. three of the strains used proved to induce chronic arthritis in the rat; all were lactobacillus casei. the cell wall of lactobacillus fermentum did not induce chronic arthritis. all arthritogenic bacterial cell walls had the same peptidoglycan structure, whereas that of l. fermentum was different. likewise, all arthritogenic cell walls we ... | 2000 | 10816508 |
| lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine. | a new lactobacillus species from pig small intestine has been identified. in an attempt to isolate lactobacillus reuteri strains carrying the putative colonization-factor gene (mub, for mucus binding) a mub-derived gene probe was used to screen pig intestinal material. a number of isolates were obtained and primary characterization showed that they were gram-positive, catalase-negative, non-spore-forming, non-motile rods. growth occurred at 45 degrees c but not at 15 degrees c and the dna g+c co ... | 2000 | 10826811 |
| pharmacokinetics of lactobacillus plantarum ncimb 8826, lactobacillus fermentum kld, and lactococcus lactis mg 1363 in the human gastrointestinal tract. | genetically modified lactic acid bacteria may be a way to deliver vaccinal epitopes in the gastrointestinal tract. | 2000 | 10848668 |
| rapid identification of 11 human intestinal lactobacillus species by multiplex pcr assays using group- and species-specific primers derived from the 16s-23s rrna intergenic spacer region and its flanking 23s rrna. | rapid and reliable two-step multiplex polymerase chain reaction (pcr) assays were established to identify human intestinal lactobacilli; a multiplex pcr was used for grouping of lactobacilli with a mixture of group-specific primers followed by four multiplex pcr assays with four sorts of species-specific primer mixtures for identification at the species level. primers used were designed from nucleotide sequences of the 16s-23s rrna intergenic spacer region and its flanking 23s rrna gene of membe ... | 2000 | 10856652 |
| analysis of promoter sequences from lactobacillus and lactococcus and their activity in several lactobacillus species. | promoter-active fragments were isolated from the genome of the probiotic organism lactobacillus rhamnosus strain gg using the promoter-probe vector pnz272. these promoter elements, together with a promoter fragment isolated from the vaginal strain lactobacillus fermentum br11 and two previously defined promoters (lactococcus lactis and lactobacillus acidophilus atcc 4356 slpa), were introduced into three strains of lactobacillus. primer-extension analysis was used to map the transcriptional star ... | 2000 | 10896218 |
| effect of probiotic bacteria on prevalence of yeasts in oropharyngeal biofilms on silicone rubber voice prostheses in vitro. | the proliferation of yeasts in the mixed bacterial and fungal biofilms colonising silicone rubber voice prostheses in laryngectomised patients is the main cause of malfunctioning of the valve mechanism on the oesophageal side of the prostheses. indwelling voice prostheses usually have to be replaced every 3-4 months. the consumption of probiotic bacteria is largely motivated by health claims related to the urogenital and lower digestive tract, but not to the upper digestive tract. the present st ... | 2000 | 10933256 |
| biochemical characterization of lactic acid bacteria isolated from spontaneous fermentation of 'almagro' eggplants. | a total of 149 strains of lactic acid bacteria isolated from the spontaneous fermentation of 'almagro' eggplants were characterized and identified. of the isolates, 148 were determined as belonging to the genus lactobacillus. a coccoid, gram-positive database-negative isolate was obtained in the early stages of fermentation. the lactobacillus strains were divided into six groups based on sugar fermentation patterns and other physiological and morphological characteristics, and were identified as ... | 2000 | 10946834 |
| activity of cecropin p1 and fa-ll-37 against urogenital microflora. | two mammalian antimicrobial peptides, fa-ll-37 and cecropin p1, were tested for activity against six uropathogens and five lactobacillus strains by broth microdilution assay. both peptides inhibited escherichia coli at 25 microm (fa-ll-39), and 1.56 microm (cecropin p1), pseudomonas aeruginosa (12.5 microm, and 25 microm), and klebsiella pneumoniae, (50 microm, and 1.56 microm), but not enterococcus faecalis and staphylococcus epidermidis. fa-ll-37 acted bacteriocidally against e. coli and bacte ... | 2000 | 10955957 |
| effect of intranasal administration of lactobacillus fermentum on the respiratory tract of mice. | this paper studied the effect of intranasal inoculation of lactobacillus fermentum, a microorganism belonging to the normal flora of the mouse pharynx, on the respiratory tract of mice. optimal temporary colonization in different areas of the tract was obtained through administration of 4 times a dose of 5 x 10(7) cfu. l. fermentum remained in the trachea and bronchi up to the 7th day after inoculation. re-inoculation of lactobacilli on the 10th day produced a transient colonization of the respi ... | 2000 | 10963306 |
| microbial community dynamics during production of the mexican fermented maize dough pozol. | the dynamics of the microbial community responsible for the traditional fermentation of maize in the production of mexican pozol was investigated by using a polyphasic approach combining (i) microbial enumerations with culture media, (ii) denaturing gradient gel electrophoresis (dgge) fingerprinting of total community dna with bacterial and eukaryotic primers and sequencing of partial 16s ribosomal dna (rdna) genes, (iii) quantification of rrnas from dominant microbial taxa by using phylogenetic ... | 2000 | 10966374 |
| purification and characterization of a surface-binding protein from lactobacillus fermentum rc-14 that inhibits adhesion of enterococcus faecalis 1131. | lactobacilli have been shown to be important in the maintenance of the healthy urogenital flora. one strain, lactobacillus fermentum rc-14, releases surface-active components which can inhibit adhesion of uropathogenic bacteria. using a quantitative method for determining inhibition of adhesion, a protein with high anti-adhesive properties against enterococcus faecalis 1131 was purified. the n-terminal sequence of the 29-kda protein was identical to that of a collagen-binding protein from lactob ... | 2000 | 10981710 |
| identification of collagen-binding proteins in lactobacillus spp. with surface-enhanced laser desorption/ionization-time of flight proteinchip technology. | biosurfactants produced by lactobacillus fermentum rc-14, l. rhamnosus gr-1 and 36, and l. casei shirota were found to contain proteins that bind to both collagen types iii and vi, as determined by surface-enhanced laser desorption/ionization (seldi)-time of flight mass spectrometry. both collagen types iii and vi immobilized on seldi preactivated proteinchip arrays detected several different sizes (2 to 48 kda) of collagen-binding proteins. overall, the rc-14-produced biosurfactant contained th ... | 2000 | 11010889 |
| probiotic therapy and functional foods for prevention of urinary tract infections: state of the art and science. | in the past year, interest has heightened in the potential for probiotics to prevent urinary tract infection (uti). mainly, this has been due to concerns about antibiotic resistance and recognition of the scientific efficacy of probiotics. the critical factors in any successful application of probiotics to patient care are the scientific basis for selecting probiotic strains and clinical verification that they are effective against the recurrence of uti. three strains--lactobacillus rhamnosus gr ... | 2000 | 11095902 |
| reduction of fecal shedding of enterohemorrhagic escherichia coli o157:h7 in lambs by feeding microbial feed supplement. | enterohemorrhagic escherichia coli o157:h7, an emerging food-borne pathogen, has been implicated in several outbreaks in the us. ruminants, including cattle, sheep and deer are reservoirs of e. coli o157:h7 and fecal shedding of the pathogen forms the vehicle of entry into the human food chain. we studied the efficacy of lactobacillus acidophilus, streptococcus faecium, a mixture of l. acidophilus and s. faecium and a mixture of l. acidophilus, s. faecium, lactobacillus casei, lactobacillus ferm ... | 2001 | 11163713 |
| oral probiotics can resolve urogenital infections. | we report the first clinical evidence that probiotic lactobacilli can be delivered to the vagina following oral intake. in 10 women with a history of recurrent yeast vaginitis, bacterial vaginosis (bv) and urinary tract infections, strains lactobacillus rhamnosus gr-1 and lactobacillus fermentum rc-14 suspended in skim milk and given twice daily for 14 days, were recovered from the vagina and identified by morphology and molecular typing within 1 week of commencement of therapy. in six cases of ... | 2001 | 11172991 |
| experimental administration of estradiol on the colonization of lactobacillus fermentum and escherichia coli in the urogenital tract of mice. | the effect of estrogen on the microbial colonization of the urogenital tract is widely discussed, mainly in regard to women with a high incidence of urinary tract infections (uti). the aim of this work was to study the effect of estradiol on the microbial colonization of lactobacilli and e. coli in mice. female balb/c mice were intramuscularly (i.m.) treated with beta-estradiol (one or three doses). the next day, l. fermentum was inoculated intraurethrally with three doses of 10(7) cfu (colony f ... | 2001 | 11217078 |
| association of lactobacillus spp. with peyer's patches in mice. | sixteen strains of lactobacillus isolated from humans, mice, and food products were screened for their capacity to associate with peyer's patches in mice. in preliminary experiments, in vitro binding to tissue pieces was assessed by scanning electron microscopy, and it was demonstrated qualitatively that 5 of the 16 strains showed some affinity for the peyer's patches, irrespective of their association with the nonlymphoid intestinal tissue. lactobacillus fermentum kld was selected for further s ... | 2001 | 11238215 |
| characterization of lactobacilli from scotch malt whisky distilleries and description of lactobacillus ferintoshensis sp. nov., a new species isolated from malt whisky fermentations. | sixty-four strains of lactobacillus were isolated from fermentation samples from 23 malt whisky distilleries located in the major whisky producing regions of scotland. the strains were assigned to 26 ribotype patterns. strains of some ribotype patterns were widely distributed and recovered from distilleries throughout scotland, while strains representing other ribotypes were particular to a specific region or even a certain distillery. repeated sampling of a single distillery over a 12 month per ... | 2001 | 11283296 |
| [the influence of anaerobic bacteria and mycoplasma isolated from the genital tract on mast cell activation]. | nowadays, it is known that mast cells, numerously appearing in all organs and being a source of a wide range of mediators and cytokines, are involved both in physiological and pathological processes. the aim of our study was to examine whether vaginal bacteria, especially those participating in bacterial vaginosis, are able to activate mast cells to mediators secretion. the study was done on rat peritoneal mast cells. the mast cells were incubated in vitro with suspensions of bacteroides capillo ... | 2000 | 11286181 |
| nutritional requirements and simplified cultivation medium to study growth and energetics of a sourdough lactic acid bacterium lactobacillus fermentum ogi e1 during heterolactic fermentation of starch. | nutritional requirements of lactobacillus fermentum ogi e1 were studied in order to define a simplified fermentation medium. | 2001 | 11309060 |
| active site residues of glutamate racemase. | glutamate racemase, muri, catalyzes the interconversion of glutamate enantiomers in a cofactor-independent fashion and provides bacteria with a source of d-glu for use in peptidoglycan biosynthesis. the enzyme uses a "two-base" mechanism involving a deprotonation of the substrate at the alpha-position to form an anionic intermediate, followed by a reprotonation in the opposite stereochemical sense. in the lactobacillus fermenti enzyme, cys73 is responsible for the deprotonation of d-glutamate, a ... | 2001 | 11371180 |
| membrane permeabilization in relation to inactivation kinetics of lactobacillus species due to pulsed electric fields. | membrane permeabilization due to pulsed electric field (pef) treatment of gram-positive lactobacillus cells was investigated by using propidium iodide uptake and single-cell analysis with flow cytometry. electric field strength, energy input, treatment time, and growth phase affected membrane permeabilization of lactobacillus plantarum during pef treatment. a correlation between pef inactivation and membrane permeabilization of l. plantarum cells was demonstrated, whereas no relationship was obs ... | 2001 | 11425727 |
| evaluation of nitric oxide production by lactobacilli. | six strains of lactobacillus fermentum and lactobacillus plantarum were investigated for nitric oxide (no) production. first, the potential presence of no synthase was examined. none of the strains of l. fermentum and l. plantarum examined produced no from l-arginine under aerobic conditions. interestingly, all l. fermentum strains expressed strong l-arginine deiminase activity. all l. fermentum strains produced no in mrs broth, but the no was found to be chemically derived from nitrite, which w ... | 2001 | 11549028 |
| comparative study of nine lactobacillus fermentum bacteriophages. | to investigate the basic properties of six temperate and three virulent phages, active on lactobacillus fermentum, on the basis of morphology, host ranges, protein composition and genome characterization. | 2001 | 11556903 |
| biofilm formation and contamination of cheese by nonstarter lactic acid bacteria in the dairy environment. | defects in cheese, such as undesirable flavors, gas formation, or white surface haze from calcium lactate crystals, can result from growth of nonstarter lactic acid bacteria (nslab). the potential for biofilm formation by nslab during cheese manufacturing, the effect of cleaning and sanitizing on the biofilm, and bacterial growth and formation of defects during ripening of the contaminated cheese were studied. stirred-curd cheddar cheese was made in the presence of stainless steel chips containi ... | 2001 | 11573770 |
| protective effect of intranasally inoculated lactobacillus fermentum against streptococcus pneumoniae challenge on the mouse respiratory tract. | lactic acid bacteria are increasingly used to restore the ecological equilibrium of different mucosal areas in humans and/or animals. likewise, they can be used to potentially protect against pathogenic microorganisms. in the present paper, the preventive effect of intranasally inoculated lactobacillus fermentum against challenge with streptococcus pneumoniae was studied, using a mouse experimental model. l. fermentum inoculated four times at a dose of 10(7) colony forming units per mouse was ab ... | 2001 | 11720814 |
| probiotic lactobacillus dose required to restore and maintain a normal vaginal flora. | forty-two healthy women were randomized to receive one of three encapsulated lactobacillus rhamnosus gr-1 plus lactobacillus fermentum rc-14 probiotic dosage regimens or l. rhamnosus gg by mouth each day for 28 days. however, the vaginal flora, assessed by nugent scoring, was only normal in 40% of the cases, and 14 patients had asymptomatic bacterial vaginosis. treatment with l. rhamnosus gr-1/l. fermentum rc-14 once and twice daily correlated with a healthy vaginal flora in up to 90% of patient ... | 2001 | 11750220 |
| evolution of the lactic acid bacterial community during malt whisky fermentation: a polyphasic study. | the development of the lactic acid bacterial community in a commercial malt whisky fermentation occurred in three broad phases. initially, bacteria were inhibited by strong yeast growth. fluorescence microscopy and environmental scanning electron microscopy revealed, in this early stage, both cocci and rods that were at least partly derived from the wort and yeast but also stemmed from the distillery plant. denaturing gradient gel electrophoresis (dgge) of partial 16s rrna genes and sequence ana ... | 2002 | 11772639 |
| persistence of lactobacillus fermentum rc-14 and lactobacillus rhamnosus gr-1 but not l. rhamnosus gg in the human vagina as demonstrated by randomly amplified polymorphic dna. | lactobacillus rhamnosus gr-1 and l. fermentum rc-14 are well-characterized probiotic strains with efficacy in the prevention and treatment of urogenital infections in women. the aim of the present study was to apply a molecular biology-based methodology for the detection of these strains and l. rhamnosus gg (a commercially available intestinal probiotic) in the human vagina in order to assess probiotic persistence at this site. ten healthy women inserted vaginally a capsule containing either a c ... | 2002 | 11777835 |
| the predominant microflora of nursing caries lesions. | the predominant microflora recovered from infected dentine of 52 carious teeth from 14 children with nursing caries was determined using both selective and non-selective media for the isolation of specific genera and acidified media (ph 5.2) to isolate the predominant aciduric microorganisms, and compared with the microflora of sound enamel surfaces in caries-free children. streptococcus mutans formed a significantly greater proportion of the lesion flora while streptococcus oralis, streptococcu ... | 2001 | 11799279 |
| rapid identification of probiotic lactobacillus biosurfactant proteins by proteinchip tandem mass spectrometry tryptic peptide sequencing. | a novel proteinchip-interfaced tandem mass spectrometer was employed to identify collagen binding proteins from biosurfactant produced by lactobacillus fermentum rc-14. on-chip tryptic digestion of the captured collagen binding proteins resulted in rapid sequence identification of five novel tryptic peptide sequences via collision-induced dissociation tandem mass spectrometry. | 2002 | 11823248 |
| new efficient amylase-producing strains of lactobacillus plantarum and l. fermentum isolated from different nigerian traditional fermented foods. | amylolytic lactic acid bacteria (alab) were isolated from nigerian traditional fermented foods (fufu, burukutu, ogi-baba and kunu-zakki) with the aim of selecting efficient amylase-producing strains. nine isolates were characterized on the basis of their phenotypic and taxo-molecular characteristics. three groups could be distinguished by their fermentation profiles and this was confirmed by dna restriction analysis. though fermentation profiles gave good identification of strain k9 (unique repr ... | 2002 | 11843413 |
| two antioxidative lactobacilli strains as promising probiotics. | two antioxidative strains tentatively identified as lactobacillus fermentum, e-3 and e-18, were isolated from intestinal microflora of a healthy child. survival time of these strains in the presence of reactive oxygen species (ros), like hydrogen peroxide, superoxide anions and hydroxyl radicals, was significantly increased compared with a non-antioxidative strain, and also was quite similar to a highly ros resistant strain of salmonella typhimurium. e-3 and e-8 contain a remarkable level of glu ... | 2002 | 11845820 |
| the potential of a polyphasic pcr-dgge approach in evaluating microbial diversity of natural whey cultures for water-buffalo mozzarella cheese production: bias of culture-dependent and culture-independent analyses. | a polyphasic pcr-dgge approach was used to describe the microbial population occurring in natural whey cultures (nwcs) for water-buffalo mozzarella cheese production. total microbial community was assessed without cultivation by analyzing dna directly extracted from the original samples of nwc. in addition, dna extracted from bulks of cells formed by harvesting colonies from the serial dilution agar plates of a variety of culture media was used to profile the "cultivable" community. the 16s rdna ... | 2001 | 11876368 |
| molecular analysis of bacterial species associated with childhood caries. | although substantial epidemiologic evidence links streptococcus mutans to caries, the pathobiology of caries may involve more complex communities of bacterial species. molecular methods for bacterial identification and enumeration now make it possible to more precisely study the microbiota associated with dental caries. the purpose of this study was to compare the bacteria found in early childhood caries (ecc) to those found in caries-free children by using molecular identification methods. clon ... | 2002 | 11880430 |
| diversity, dynamics, and activity of bacterial communities during production of an artisanal sicilian cheese as evaluated by 16s rrna analysis. | the diversity and dynamics of the microbial communities during the manufacturing of ragusano cheese, an artisanal cheese produced in sicily (italy), were investigated by a combination of classical and culture-independent approaches. the latter included pcr, reverse transcriptase-pcr (rt-pcr), and denaturing gradient gel electrophoresis (dgge) of 16s rrna genes (rdna). bacterial and lactobacillus group-specific primers were used to amplify the v6 to v8 and v1 to v3 regions of the 16s rrna gene, r ... | 2002 | 11916708 |
| isolation and characterization of lactic acid bacteria from feces of newborn baby and from dongchimi. | lactic acid bacteria were screened from feces of newborn baby and from dongchimi. selection criteria employed included the ability of strains to withstand environmental conditions such as low ph, high bile concentration, and oxygen. the isolates were applied to the juice of various vegetables, and fermentabilities of isolates were compared. strains f20-3, f35-3, and f35-6 showed high stability compared to the other strains at ph 3.0 and 2.3. strains d1 and d2 showed the highest survival at ph 3. ... | 2002 | 11958617 |
| growth studies of potentially probiotic lactic acid bacteria in cereal-based substrates. | the overall growth kinetics of four potentially probiotic strains (lactobacillus fermentum, lact. reuteri, lact. acidophilus and lact. plantarum) cultured in malt, barley and wheat media were investigated. the objectives were to identify the main factors influencing the growth and metabolic activity of each strain in association with the cereal substrate. | 2002 | 11972688 |