| lactic acid bacteria in dried vegetables and spices. | spices and dried vegetable seasonings are potential sources of bacterial contamination for foods. however, little is known about lactic acid bacteria (lab) in spices and dried vegetables, even though certain lab may cause food spoilage. in this study, we enumerated lab in 104 spices and dried vegetables products aimed for the food manufacturing industry. the products were obtained from a spice wholesaler operating in finland, and were sampled during a one-year period. we picked isolates (n = 343 ... | 2016 | 26678137 |
| molecular cloning of kman coding for mannanase from klebsiella oxytoca kub-cw2-3 and its hybrid mannanase characters. | gene encoding for β-mannanase (e.c 3.2.1.78) from klebsiella oxytoca kub-cw2-3 was cloned and expressed by an e. coli system resulting in 400 times higher mannanase activities than the wild type. a 3314bp dna fragment obtained revealed an open reading frame of 1164bp, namely kman-2, which encoded for 387 amino acids with an estimated molecular weight of 43.2kda. it belonged to the glycosyl hydrolase family 26 (gh26) exhibited low similarity of 50-71% to β-mannanase produced by other microbial so ... | 2016 | 27233126 |
| identification and sequence analysis of pwcmbf8-1, a bacteriocin-encoding plasmid from the lactic acid bacterium weissella confusa. | members of the gram-positive lactic acid bacteria (lab) are well-known for their beneficial properties as starter cultures and probiotics. many lab species produce ribosomally synthesized proteinaceous antibiotics (bacteriocins). weissella confusa mbf8-1 is a strain isolated from a fermented soybean product that not only produces useful exopolysaccharides but also exhibits bacteriocin activity, which we call weissellicin mbf. here, we show that bacteriocin production by w. confusa mbf8-1 is spec ... | 2016 | 26976853 |
| construction of a shuttle vector based on the small cryptic plasmid pjy33 from weissella cibaria 33. | a cryptic plasmid, pjy33, from weissella cibaria 33 was characterized. pjy33 was 2365 bp in size with a gc content of 41.27% and contained two putative open reading frames (orfs). orf1 encoded a putative hypothetical protein of 134 amino acids. orf2 was 849 bp in size, and its putative translation product exhibited 87% identity with a replication initiation factor from a plasmid from w. cibaria klc140. a weissella-escherichia coli shuttle vector, pjy33e (6.5 kb, em(r)), was constructed by ligati ... | 2015 | 25882072 |
| challenges in analysis of high-molar mass dextrans: comparison of hpsec, asflfff and dosy nmr spectroscopy. | dilute solutions of various dextran standards, a high-molar mass (hmm) commercial dextran from leuconostoc spp., and hmm dextrans isolated from weissella confusa and leuconostoc citreum were analyzed with high-performance size-exclusion chromatography (hpsec), asymmetric flow field-flow fractionation (asflfff), and diffusion-ordered nmr spectroscopy (dosy). hpsec analyses were performed in aqueous and dimethyl sulfoxide (dmso) solutions, while only aqueous solutions were utilized in asflfff and ... | 2014 | 24274497 |
| distinctive intestinal lactobacillus communities in 6-month-old infants from rural malawi and southwestern finland. | our aim was to compare the composition and diversity of lactobacillus microbiota in infants living in malawi and southwestern finland. | 2015 | 26049782 |
| stress response of some lactic acid bacteria isolated from romanian artisan dairy products. | understanding the mechanisms of stress response and adaptation to stress in the case of lactic acid bacteria (lab), especially in the case of strains with functional properties, is very important when such strains are potential candidates for starter cultures or probiotics. in this context, our study shows the response of some lab [four exopolysaccharide (eps)-producing strains and one strain with potential probiotic effect] to the stresses induced by low and high incubation temperatures, acidit ... | 2014 | 23933743 |
| reply to "confused by weissella confusa bacteremia". | | 2015 | 25976900 |
| confused by weissella confusa bacteremia. | | 2015 | 25976899 |
| photo quiz: confusing bacteremia in a crohn's disease patient. weissella confusa. | | 2015 | 25700525 |
| community dynamics and metabolite target analysis of spontaneous, backslopped barley sourdough fermentations under laboratory and bakery conditions. | barley flour is not commonly used for baking because of its negative effects on bread dough rheology and loaf volume. however, barley sourdoughs are promising ingredients to produce improved barley-based breads. spontaneous barley sourdough fermentations were performed through backslopping (every 24h, 10days) under laboratory (fermentors, controlled temperature of 30°c, high dough yield of 400) and bakery conditions (open vessels, ambient temperature of 17-22°c, low dough yield of 200), making u ... | 2016 | 27088869 |
| in vitro modulation of tumor necrosis factor α production in thp-1 cells by lactic acid bacteria isolated from healthy human infants. | the human microbiota is a source of probiotics capable of modulating the host immune system. in this study, we collected fecal samples from 100 healthy infants and isolated lactic acid bacteria which were screened for immune modulating effects on tumor necrosis factor α (tnf-α) production. cell-free culture supernatants from 26 isolates were able to decrease tnf-α production in vitro and three of the isolates were selected as candidate probiotics (msmc39-1, msmc39-3, msmc57-1). these isolates we ... | 2015 | 25759008 |
| weissella confusa: problems with identification of an opportunistic pathogen that has been found in fermented foods and proposed as a probiotic. | weissella confusa is found in fermented foods and has been suggested as a probiotic, but also causes sepsis and other serious infections in humans and animals. the incidence of human infections is underestimated partly due to confusion with viridans streptococci and partly due to difficulty making a definitive identification, even if the organism is recognized to belong to another genus, owing to the inability of commercial organism systems to identify it. we report our experiences identifying w ... | 2014 | 24971076 |
| genomics of weissella cibaria with an examination of its metabolic traits. | weissella is a genus of lactic acid bacteria (lab) consisting of species formerly included in the leuconostoc paramesenteroides group. similar to other lab, they are commonly found in fermented foods but have also been isolated from environmental and human samples. currently there are 20 recognized species. herein, three weissella cibaria genomes were sequenced using illumia mi-seq and roche 454 technologies. annotation was performed using the prokka and jgi img pipelines. a thorough analysis of ... | 2015 | 25678547 |
| characterization of exopolysaccharide and ropy capsular polysaccharide formation by weissella. | with their broad functional properties, lactic acid bacteria derived high molar mass exopolysaccharides (eps) and oligosaccharides are of great interest for food, medical and pharmaceutical industry. eps formation by 123 strains of weissella cibaria and weissella confusa, was evaluated. dextran formation from sucrose was observed for all tested strains while 18 strains produced fructan in addition to dextran. six isolates synthesized a highly ropy polymer from glucose associated with the formati ... | 2015 | 25475311 |
| weissella confusa cab3 dextransucrase: properties and in vitro synthesis of dextran and glucooligosaccharides. | food-derived weissella spp. have gained attention during recent years as efficient dextran producers. weissella confusa cab3 dextransucrase (wccab3-dsr) was isolated applying peg fractionation and used for in vitro synthesis of dextran and glucooligosaccharides. wccab3-dsr had a molar mass of 178 kda and was activated by co(2+) and ca(2+) ions. glycerol and tween 80 enhanced enzyme stability, and its half-life at 30°c increased from 10h to 74 h and 59 h, respectively. the (1)h and (13)c nmr spec ... | 2014 | 24299811 |
| weissella confusa bacteremia in an immune-competent patient with underlying intramural hematomas of the aorta. | | 2013 | 24205498 |
| evidence for xylooligosaccharide utilization in weissella strains isolated from indian fermented foods and vegetables. | six strains isolated from fermented food were identified as weissella species by 16s rdna sequencing, clustering with the species pair w. confusa/w. cibaria. the strains were analysed for growth on glucose, xylose and xylooligosaccharides (xos). all strains were xylose positive using the api chl 50 test. growth on xos was observed for strains 85, 92, 145 and av1, firstly by optical density measurements in microtitre plates and secondly in batch cultures also confirming concomitant decrease in ph ... | 2013 | 23738850 |
| proteomic analysis of proteins of weissella confusa 31 affected by bile salts. | weissella confusa 31, an isolate from human feces, possesses desirable properties as a probiotic strain, including bile salt resistance. w. confusa 31 is not inhibited by bile salts up to 0.3% concentration. proteins affected by bile salts (0.05%) were examined by 2-d gel electrophoresis. our proteomic analyses revealed that the intensities of 29 spots were changed, where 17 increased (including 2 spots observed only under the bile salts stress conditions) and 12 decreased. proteins were identif ... | 2012 | 23075796 |
| optimization of exopolysaccharide overproduction by lactobacillus confusus in solid state fermentation under high salinity stress. | it is believed that high concentrations of sodium chloride (nacl) suppress the biosynthesis of exopolysaccharide (eps) in lactic acid bacteria (lab). nevertheless, overproduction of epss due to high salinity stress in solid state fermentation performed on an agar surface was demonstrated in this study using a response surface methodology via a central composite design (ccd). under optimized conditions with nacl 4.97% and sucrose 136.5 g/l at 40.79 h of incubation, the eps yield was 259% (86.36 g ... | 2012 | 22738958 |
| genome sequence of weissella confusa lbae c39-2, isolated from a wheat sourdough. | weissella confusa is a rod-shaped heterofermentative lactic acid bacterium from the family of leuconostocaceae. here we report the draft genome sequence of the strain w. confusa lbae c39-2 isolated from a traditional french wheat sourdough. | 2012 | 22374947 |
| optimization of electrotransformation conditions for leuconostoc mesenteroides subsp. mesenteroides atcc8293. | to establish an efficient genetic transformation protocol for leuconostoc species, methods for competent-cell preparation and electroporation conditions were optimized. | 2012 | 22897881 |
| identification of lactic acid bacteria associated with traditional cachaça fermentations. | during the production of traditional cachaça (alembic´s cachaça), contamination of the fermented must is one of the factors leading to economic losses in the beverage manufacturing industry. the diversity of bacterial populations and the role of these microorganisms during the cachaça production process are still poorly understood in brazil. in our work, the fermentation process was followed in two distilleries located in the state of minas gerais. the objective of this work was to identify the ... | 2010 | 24031520 |
| [weissella confusa bacteremia: an underestimated opportunistic pathogen]. | | 2017 | 28325577 |
| microbiology of pozol, a mexican fermented maize dough. | mexican fermented maize dough, pozol, including traditional banana leaf-wrapped samples and material in plastic bags, was purchased. all samples were ph 4.7 to 5.7 approx. 12 h after preparation, ph declining to 3.6 to 3.9 after 6 to 9 days storage at ambient temperature. these latter samples had dry matter contents of 31% to 48% (w/w), 0.35% to 0.75% titratable acidity as lactic acid and lactic acid bacteria as predominant microbial flora at about 10(8) c.f.u./ml. the lactic acid bacteria inclu ... | 1995 | 24414915 |
| draft genome sequence of weissella confusa mbf8-1, a glucansucrase- and bacteriocin-producing strain isolated from a homemade soy product. | we report here the draft genome sequence of weissella confusa mbf8-1, an isolate from a homemade fermented soybean product that produces sucrases and exhibits antibacterial (bacteriocin) activity. the draft genome of w. confusa mbf8-1 comprises a 2.2-mbp chromosome and a 17.8-kbp bacteriocin-encoding plasmid. two putative glucansucrase genes were also identified. | 2017 | 28126938 |
| physico-chemical characterization of galactan exopolysaccharide produced by weissella confusa kr780676. | physico-chemical properties of a galactan exopolysaccharide (eps) produced by weissella confusa kr780676 isolated from an idli batter were studied. in our previous paper, we had reported the isolation and characterization of a linear galactan (containing α-(1→6)-linked galactose units) isolated from an acidic fermented food. in this manuscript, we are reporting the physico-chemical properties viz., colour evaluation (l* 81.79, a* 3.15, b* 15.04), flow properties (compressibility index 38.46%), s ... | 2016 | 27645923 |
| intestinal lactobacillus community structure and its correlation with diet of southern chinese elderly subjects. | this study aimed to investigate the relationship between the intestinal lactobacillus species and diet of elderly subjects in a longevity area in southern china. healthy elderly subjects ranging from 80 to 99 years old were respectively selected from the regions of bama and nanning, guangxi, china. the nested polymerase chain reaction and denaturing gradient gel electrophoresis (pcr-dgge) technology was used to analyze the intestinal lactobacillus community structure. results showed that weissel ... | 2016 | 27572508 |
| structure modeling and functional analysis of recombinant dextransucrase from weissella confusa cab3 expressed in lactococcus lactis. | the dextransucrase gene from weissella confusa cab3, having an open reading frame of 4.2 kb coding for 1,402 amino acids, was amplified, cloned, and expressed in lactococcus lactis. the recombinant dextransucrase, wccab3-rdsr was expressed as extracellular enzyme in m17 medium with a specific activity of 1.5 u/mg which after purification by peg-400 fractionation gave 6.1 u/mg resulting in 4-fold purification. wccab3-rdsr was expressed as soluble and homogeneous protein of molecular mass, approxi ... | 2016 | 26861959 |
| cloning and characterization of a weissella confusa dextransucrase and its application in high fibre baking. | wheat bran offers health benefits as a baking ingredient, but is detrimental to bread textural quality. dextran production by microbial fermentation improves sourdough bread volume and freshness, but extensive acid production during fermentation may negate this effect. enzymatic production of dextran in wheat bran was tested to determine if dextran-containing bran could be used in baking without disrupting bread texture. the weissella confusa vtt e-90392 dextransucrase gene was sequenced and his ... | 2015 | 25603169 |
| optimization of isomaltooligosaccharide size distribution by acceptor reaction of weissella confusa dextransucrase and characterization of novel α-(1→2)-branched isomaltooligosaccharides. | long-chain isomaltooligosaccharides (imos) are promising prebiotics. imos were produced by a weissella confusa dextransucrase via maltose acceptor reaction. the inputs of substrates (i.e., sucrose and maltose, 0.15-1 m) and dextransucrase (1-10 u/g sucrose) were used to control imo yield and profile. according to response surface modeling, 1 m sucrose and 0.5 m maltose were optimal for the synthesis of longer imos, whereas the dextransucrase dosage showed no significant effect. in addition to th ... | 2016 | 27050481 |
| iranian wheat flours from rural and industrial mills: exploitation of the chemical and technology features, and selection of autochthonous sourdough starters for making breads. | this study aimed at describing the main chemical and technology features of eight iranian wheat flours collected from industrial and artisanal mills. their suitability for bread making was investigated using autochthonous sourdough starters. chemical analyses showed high concentration of fibers and ash, and technology aptitude for making breads. as shown through 2-de analyses, gliadin and glutenin subunits were abundant and varied among the flours. according to the back slopping procedure, type ... | 2015 | 25583343 |
| characterization of a novel galactan produced by weissella confusa kr780676 from an acidic fermented food. | an exopolysaccharide (eps) producing strain pufstm055 isolated from idli batter (an indian traditional cereal-legume based fermented food) was identified as weissella confusa kr780676. the strain was shown to produce 17.2g/l (dry weight) of eps in 2% sucrose supplemented mrs broth and the eps was characterized. hptlc analysis confirmed the presence of galactose monomers, indicating the homopolysaccharide nature of eps. fourier-transform infrared spectroscopy and nuclear magnetic resonance analys ... | 2016 | 26836614 |
| rye bran as fermentation matrix boosts in situ dextran production by weissella confusa compared to wheat bran. | the consumption of fiber-rich foods such as cereal bran is highly recommended due to its beneficial health effects. pre-fermentation of bran with lactic acid bacteria can be used to improve the otherwise impaired flavor and textural qualities of bran-rich products. these positive effects are attributed to enzymatic modification of bran components and the production of functional metabolites like organic acids and exopolysaccharides such as dextrans. the aim of this study was to investigate dextr ... | 2016 | 26649737 |
| exopolysaccharides from co-cultures of weissella confusa 11gu-1 and propionibacterium freudenreichii js15 act synergistically on wheat dough and bread texture. | the storage of bread is limited by both physical (staling) and microbial (mainly fungal) spoilage. exopolysaccharides (eps) produced by lactic acid bacteria (lab) and organic acids from propionibacteria (pab) have been used to enhance texture and extend shelf-life of bakery products. in this study the functionality of eps of weissella confusa a3/2-1 (dextran), w. confusa f3/2-2 (dextran and levan), w. confusa 11gu-1 (dextran and ropy capsular polysaccharide) was evaluated in wheat bread. two str ... | 2015 | 26256717 |
| structural analysis of linear mixed-linkage glucooligosaccharides by tandem mass spectrometry. | dextrans and glucooligosaccharides (glos) are produced by lactic acid bacteria (lab) during sourdough fermentation. the dextrans can act as hydrocolloids in sourdough bread, while the glos may have antistaling and prebiotic properties, depending on their structure. development of high-throughput methods for screening the structural properties of dextrans and glos produced by different lab in varying fermentation conditions is therefore of interest. in this study we explored the possibility of us ... | 2013 | 23194554 |
| antifungal activity of leuconostoc citreum and weissella confusa in rice cakes. | the antifungal activity of organic acids greatly improves the shelf life of bread and bakery products. however, little is known about the effect of lactic acid fermentation on fungal contamination in rice cakes. here, we show that lactic acid fermentation in rice dough can greatly retard the growth of three fungal species when present in rice cakes, namely cladosporium sp. ys1, neurospora sp. ys3, and penicillium crustosum ys2. the antifungal activity of the lactic acid bacteria against these fu ... | 2012 | 23124754 |
| the impact of fermentation with exopolysaccharide producing lactic acid bacteria on rheological, chemical and sensory properties of pureed carrots (daucus carota l.). | fermentation with lactic acid bacteria (lab) offers a natural means to modify technological and nutritional properties of foods and food ingredients. this study explored the impact of fermentation with different exopolysaccharide (eps) producing lab on rheological, chemical and sensory properties of puréed carrots in water, as a vegetable model, with the focus on texture formation. the screening of 37 lab strains for starter selection revealed 16 lactobacillus, leuconostoc and weissella strains ... | 2015 | 26001525 |
| identification of weissella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | although some weissella species play beneficial roles in food fermentation and in probiotic products, others such as weissella confusa are emerging gram-positive pathogens in immunocompromised hosts. weissella species are difficult to identify by conventional biochemical methods and commercial automated systems and are easily misidentified as lactobacillus and leuconostoc species. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is increasingly being us ... | 2015 | 26594208 |
| lactose- and cellobiose-derived branched trisaccharides and a sucrose-containing trisaccharide produced by acceptor reactions of weissella confusa dextransucrase. | dextran-producing weissella have received significant attention. however, except for maltose, the acceptor reactions of weissella dextransucrases with different sugars have not been investigated. the action of recombinant weissella confusa vtt e-90392 dextransucrase was tested with several potential acceptors, particularly, analogs lactose and cellobiose. the major acceptor products of both disaccharides were identified as branched trisaccharides, with a glucosyl residue α-(1 → 2)-linked to the ... | 2016 | 26212965 |
| a confusing case - weissella confusa prosthetic joint infection: a case report and review of the literature. | the authors describe the first case of weissella confusa infection of a prosthetic joint. identification of the pathogen required 16s ribosomal rna sequencing of isolates obtained on two separate occasions during the assessment of an elderly woman with a painful, swollen knee following total knee arthroplasty. a review of reported human infections due to w confusa are summarized, and risk factors and pitfalls in the application of empirical antimicrobial therapy pending definitive microbiologica ... | 2014 | 25285116 |
| modeling and investigation of submerged fermentation process to produce extracellular polysaccharide using lactobacillus confusus. | the main objective of the present study is to investigate and optimize the submerged fermentation (smf) process parameters such as addition of coconut water, nacl dose, incubation time and temperature on the production of extracellular polysaccharide (eps) and biomass production using lactobacillus confuses. response surface methodology (rsm) coupled with four factors three level box-behnken design (bbd) was employed to model the smf process. rsm analysis indicated good correspondence between ex ... | 2014 | 25263862 |
| weissella confusa septicemia in a foal. | weissella confusa is a gram-positive bacterium that has been identified in environmental and food samples from around the world. rare cases of bacteremia in immunocompromised people have been reported. a 2-day-old foal was presented for weakness and suspected sepsis. blood culture yielded pure growth of a gram-positive coccobacillus, which was identified as w. confusa through sequencing of the 16s ribosomal dna. although the foal initially responded to antimicrobial therapy with ceftiofur and me ... | 2014 | 24327736 |
| comparison of bacterial community changes in fermenting kimchi at two different temperatures using a denaturing gradient gel electrophoresis analysis. | a polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) technique followed by sequencing of the 16s rdna fragments eluted from the bands of interest on denaturing gradient gels was used to monitor changes in the bacterial microflora of two commercial kimchi, salted cabbage, and ingredient mix samples during 30 days of fermentation at 4°c and 10°c. leuconostoc (lc.) was the dominant lactic acid bacteria (lab) over lactobacillus (lb.) species at 4°c. weissella confusa was de ... | 2013 | 23314371 |
| exopolysaccharides from lactic acid bacteria: structural analysis, molecular weight effect on immunomodulation. | exopolysaccharides (eps) obtained from the culture medium of lactobacillus confusus tistr 1498 were investigated to determine their molecular characteristics and the effect of molecular weight (mw) on immunomodulatory activity. the eps mainly consisted of carbohydrates (81.9±2.4%) with only one type of monosaccharide, d-glucose, which was mostly connected by α-(1→6) glycosidic linkages. the eps itself was unable to stimulate raw264.7 cells to produce pro-inflammatory mediator nitric oxide (no) a ... | 2014 | 24820155 |