| butanol production by clostridium beijerinckii. part i: use of acid and enzyme hydrolyzed corn fiber. | fermentation of sulfuric acid treated corn fiber hydrolysate (sacfh) inhibited cell growth and butanol production (1.7+/-0.2g/l acetone butanol ethanol or abe) by clostridium beijerinckii ba101. treatment of sacfh with xad-4 resin removed some of the inhibitors resulting in the production of 9.3+/-0.5 g/l abe and a yield of 0.39+/-0.015. fermentation of enzyme treated corn fiber hydrolysate (etcfh) did not reveal any cell inhibition and resulted in the production of 8.6+/-1.0 g/l abe and used 24 ... | 2008 | 18061440 |
| nitrogen-fixation genes and nitrogenase activity in clostridium acetobutylicum and clostridium beijerinckii. | several solvent-producing clostridia, including clostridium acetobutylicum and c. beijerinckii, were previously shown to be nitrogen-fixing organisms based on the incorporation of 15n2 into cellular material. the key nitrogen-fixation (nif) genes, including nifh, nifd, and nifk for nitrogenase component proteins as well as nife, nifn, nifb and nifv for synthesis of the iron-molybdenum cofactor (femoco) of nitrogenase, have now been identified in c. acetobutylicum or c. beijerinckii or both. the ... | 2001 | 11781802 |
| recent advances in abe fermentation: hyper-butanol producing clostridium beijerinckii ba101. | this is an overview of the mutant strain clostridium beijerinckii ba101 which produces solvents (acetone-butanol-ethanol, abe) at elevated levels. this organism expresses high levels of amylases when grown on starch. c. beijerinckii ba101 hydrolyzes starch effectively and produces solvent in the concentration range of 27-29 g x l(-1). c. beijerinckii ba101 has been characterized for both substrate and butanol inhibition. supplementing the fermentation medium (mp2) with sodium acetate enhances so ... | 2001 | 11781803 |
| expression of solvent-forming enzymes and onset of solvent production in batch cultures of clostridium beijerinckii ("clostridium butylicum"). | clostridium beijerinckii ("clostridium butylicum") nrrl b592 and nrrl b593 were grown in batch cultures without ph control. the use of more sensitive and accurate procedures for the determination of solvents in cultures led to the recognition of the onset of solvent production about 2 h earlier than the previously assigned point and at a higher culture ph for both strains. reliable assays for solvent-forming enzyme activities in cell extracts have also been developed. the results showed that act ... | 1988 | 16347575 |
| long-term continuous cultivation of clostridium beijerinckii in a two-stage chemostat with on-line solvent removal. | a two-stage continuous cultivation experiment with clostridium beijerinckii nrrl b592 is described. this strain maintained its ability to produce neutral solvents (acetone, n-butanol, and ethanol) at an overall dilution rate of 0.13 h(sup-1) and achieved an average overall solvent concentration of 9.27 g/liter and an overall solvent productivity of 1.24 g/liter/h for more than 100 overall retention times. the experiment was performed without ph control on a semisynthetic medium containing yeast ... | 1996 | 16535396 |
| assignment of the six solvent-producing clostridium spp. to clostridium beijerinckii based on dna-dna reassociation. | | 1999 | 12501369 |
| transcriptional analysis of clostridium beijerinckii ncimb 8052 and the hyper-butanol-producing mutant ba101 during the shift from acidogenesis to solventogenesis. | clostridium beijerinckii is an anaerobic bacterium used for the fermentative production of acetone and butanol. the recent availability of genomic sequence information for c. beijerinckii ncimb 8052 has allowed for an examination of gene expression during the shift from acidogenesis to solventogenesis over the time course of a batch fermentation using a ca. 500-gene set dna microarray. the microarray was constructed using a collection of genes which are orthologs of members of gene families prev ... | 2008 | 18849451 |
| metabolic engineering of saccharomyces cerevisiae for the production of n-butanol. | abstract: background: increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol. results and conclusion: saccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (s. cerevisiae, escherichia coli, clostridium beijerinckii, and ralstonia eutropha) were substituted for the clostridial enzymes and their ... | 2008 | 19055772 |
| detoxification of model phenolic compounds in lignocellulosic hydrolysates with peroxidase for butanol production from clostridium beijerinckii. | in the present study, we investigated the peroxidase-catalyzed detoxification of model phenolic compounds and evaluated the inhibitory effects of the detoxified solution on butanol production by clostridium beijerinckii national collection of industrial and marine bacteria ltd. 8052. the six phenolic compounds, p-coumaric acid, ferulic acid, 4-hydroxybenzoic acid, vanillic acid, syringaldehyde, and vanillin, were selected as model fermentation inhibitors generated during pretreatment and hydroly ... | 2009 | 19300996 |
| fermentation of rice bran and defatted rice bran for butanol 5 production using clostridium beijerinckii ncimb 8052. | we examined butanol fermentation by clostridium beijerinckii ncimb 8052 using various hydrolyzates obtained from rice bran, which is one of the most abundant agricultural by-products in korea and japan. in order to increase the amount of fermentable sugars in the hydrolyzates of rice bran, various hydrolysis procedures were applied. eight different hydrolyzates were prepared using rice bran (rb) and defatted rice bran (drb) with enzyme or acid treatment or both. each hydrolyzate was evaluated in ... | 2009 | 19494696 |
| isolation of a new butanol-producing clostridium strain: high level of hemicellulosic activity and structure of solventogenesis genes of a new clostridium saccharobutylicum isolate. | new isolates of solventogenic bacteria exhibited high hemicellulolytic activity. they produced butanol and acetone with high selectivity for butanol (about 80% of butanol from the total solvent yield). their 16s rdna sequence was 99% identical to that of clostridium saccharobutylicum. the genes responsible for the last steps of solventogenesis and encoding crotonase, butyryl-coa dehydrogenase, electron-transport protein subunits a and b, 3-hydroxybutyryl-coa dehydrogenase, alcohol dehydrogenase, ... | 2009 | 19674858 |
| identification and characterization of two novel clostridial bacteriocins, circularin a and closticin 574. | two novel antibacterial peptides of clostridial species were purified, n-terminally sequenced, and characterized. moreover, their structural genes were identified. closticin 574 is an 82-amino-acid bacteriocin produced by clostridium tyrobutyricum adriat 932. the supernatant of the producing strain showed a high level of activity against the indicator strain c. tyrobutyricum. the protein is synthesized as a preproprotein that is possibly secreted via the general secretion pathway, after which it ... | 2003 | 12620847 |
| continuous production of butanol from starch-based packing peanuts. | acetone, butanol, ethanol (abe, or solvents) were produced from starch-based packing peanuts in batch and continuous reactors. in a batch reactor, 18.9 g/l of total abe was produced from 80 g/l packing peanuts in 110 h of fermentation. the initial and final starch concentrations were 69.6 and 11.1 g/l, respectively. in this fermentation, abe yield and productivity of 0.32 and 0.17 g/(l h) were obtained, respectively. compared to the batch fermentation, continuous fermentation of 40 g/l of starch ... | 2003 | 12721460 |
| biochemical and structural properties of chimeras constructed by exchange of cofactor-binding domains in alcohol dehydrogenases from thermophilic and mesophilic microorganisms. | the cofactor-binding domains (residues 153-295) of the alcohol dehydrogenases from the thermophile thermoanaerobacter brockii (tbadh), the mesophilic bacterium clostridium beijerinckii (cbadh), and the protozoan parasite entamoeba histolytica (ehadh1) have been exchanged. three chimeras have been constructed. in the first chimera, the cofactor-binding domain of thermophilic tbadh was replaced with the cofactor-binding domain of its mesophilic counterpart cbadh [chimera chi21((tct))]. this domain ... | 2010 | 20102159 |
| functional expression of the thiolase gene thl from clostridium beijerinckii p260 in lactococcus lactis and lactobacillus buchneri. | the first step of the butanol pathway involves an acetyl-coa acetyltransferase (acoaat), which controls the key branching point from acetyl-coa to butanol. acoaat, also known as thiolase (ec 2.3.1.9), is encoded by the thl gene and catalyzes ligation of two acetyl-coa into acetoacetyl-coa. bioinformatics analyses suggest there are no thl in the genomes of lactic acid bacteria (lab), in this study we aimed to introduce the thl gene into selected lab strains and analyze the fermentation products. ... | 2010 | 20371307 |
| acetone butanol ethanol (abe) production from concentrated substrate: reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping. | acetone butanol ethanol (abe) was produced in an integrated fed-batch fermentation-gas stripping product-recovery system using clostridium beijerinckii ba101, with h(2) and co(2) as the carrier gases. this technique was applied in order to eliminate the substrate and product inhibition that normally restricts abe production and sugar utilization to less than 20 g l(-1) and 60 g l(-1), respectively. in the integrated fed-batch fermentation and product recovery system, solvent productivities were ... | 2004 | 12910325 |
| the t box regulatory element controlling expression of the class i lysyl-trna synthetase of bacillus cereus strain 14579 is functional and can be partially induced by reduced charging of asparaginyl-trnaasn. | lysyl-trna synthetase (lysrs) is unique within the aminoacyl-trna synthetase family in that both class i (lysrs1) and class ii (lysrs2) enzymes exist. lysrs1 enzymes are found in archaebacteria and some eubacteria while all other organisms have lysrs2 enzymes. all sequenced strains of bacillus cereus (except ah820) and bacillus thuringiensis however encode both a class i and a class ii lysrs. the lysk gene (encoding lysrs1) of b. cereus strain 14579 has an associated t box element, the first rep ... | 2010 | 20649968 |
| crystalline alcohol dehydrogenases from the mesophilic bacterium clostridium beijerinckii and the thermophilic bacterium thermoanaerobium brockii: preparation, characterization and molecular symmetry. | two tetrameric nadp(+)-dependent bacterial secondary alcohol dehydrogenases have been crystallized in the apo- and the holo-enzyme forms. crystals of the holo-enzyme from the mesophilic clostridium beijerinckii (ncbad) belong to space group p2(1)2(1)2(1) with unit-cell dimensions a = 90.5, b = 127.9, c = 151.4 a. crystals of the apo-enzyme (cbad) belong to the same space group with unit-cell dimensions a = 80.4, b = 102.3, c = 193.5 a. crystals of the holo-enzyme from the thermophilic thermoanae ... | 1996 | 15299659 |
| continuous butanol fermentation and feed starch retrogradation: butanol fermentation sustainability using clostridium beijerinckii ba101. | use of starch solution as feed for butanol bioconversion processes employing clostridium beijerinckii ba101 may have added economic advantage over the use of glucose. acetone butanol ethanol (abe) was produced from 30 gl(-1) starch solution using a continuous process. the bioreactor was fed at a dilution rate of 0.02 h(-1) and starch solution/feed volume (3 l) was replaced every 72 h. the continuous reactor fed with cornstarch solution (feed temperature 19 degrees c) produced approximately 6.0 g ... | 2005 | 15607236 |
| d-2,3-butanediol production due to heterologous expression of an acetoin reductase in clostridium acetobutylicum. | acetoin reductase (acr) catalyzes the conversion of acetoin to 2,3-butanediol. under certain conditions, clostridium acetobutylicum atcc 824 (and strains derived from it) generates both d- and l-stereoisomers of acetoin, but because of the absence of an acr enzyme, it does not produce 2,3-butanediol. a gene encoding acr from clostridium beijerinckii ncimb 8052 was functionally expressed in c. acetobutylicum under the control of two strong promoters, the constitutive thl promoter and the late exp ... | 2011 | 21335380 |
| continuous production of 1,3-propanediol using raw glycerol with immobilized clostridium beijerinckii nrrl b-593 in comparison to suspended culture. | the continuous production of 1,3-propanediol (1,3-pdo) was investigated with clostridium beijerinckii nrrl b-593 using raw glycerol without purification obtained from a biodiesel production process. ceramic rings and pumice stones were used for cell immobilization in a packed-bed bioreactor. for comparison purpose, a control bioreactor with suspended culture was also run. the effect of hydraulic retention time (hrt) on the production of 1,3-pdo in both immobilized and suspended bioreactors were ... | 2011 | 21336641 |
| maintenance of deltaph by a butanol-tolerant mutant of clostridium beijerinckii. | the isolation of clostridium beijerinckii mutants that are more tolerant of butanol than the wild-type offered the opportunity to investigate whether the membrane activities which are required for maintaining the transmembrane deltaph (the difference in ph between the cellular interior and exterior) are sensitive targets of butanol toxicity. the deltaph was measured by the accumulation of [14c]benzoate using late-exponential-phase cells which were suspended in citrate/phosphate buffer at ph 5 (t ... | 2005 | 15699209 |
| acetone, isopropanol, and butanol production by clostridium beijerinckii (syn. clostridium butylicum) and clostridium aurantibutyricum. | thirty-four strains representing 15 species of anaerobic bacteria were screened for acetone, isopropanol, and n-butanol (solvent) production. under our culture conditions, several strains of clostridium beijerinckii and c. aurantibutyricum produced at least 40 mm n-butanol (c. acetobutylicum strains produced up to 41 mm n-butanol under similar conditions). both solvent-producing and non-solvent-producing strains of c. beijerinckii have high dna homology with a reference strain of c. beijerinckii ... | 1983 | 16346237 |
| acidic conditions are not obligatory for onset of butanol formation by clostridium beijerinckii (synonym, c. butylicum). | factors that may initiate the metabolic transition for butanol production were investigated in batch cultures of clostridium beijerinckii (synonym, clostridium butylicum) vpi 13436. cultures maintained at ph 6.8 produced nearly as much butanol as those incubated without ph control, indicating that neither a change in the culture ph nor acid conditions per se are always required to initiate solvent formation. acetate and butyrate levels at the onset of butanol production were dependent on the ph ... | 1983 | 16346358 |
| soy molasses as fermentation substrate for production of butanol using clostridium beijerinckii ba101. | spray-dried soy molasses (sdsm) contains the sugars dextrose, sucrose, fructose, pinitol, raffinose, verbascose, melibiose, and stachyose. of the 746 g kg(-1) total sugars in sdsm, 434 g kg(-1) is fermentable using clostridium beijerinckii ba101. sdsm was used to produce acetone, butanol, and ethanol (abe) by c. beijerinckii ba101 in batch cultures. using 80 g l(-1) sdsm, 10.7 g l(-1) abe was produced in p2 medium. higher concentrations of sdsm resulted in poor solvent production due to the pres ... | 2001 | 11494105 |
| alanine-scanning of the 50's loop in the clostridium beijerinckii flavodoxin: evaluation of additivity and the importance of interactions provided by the main chain in the modulation of the oxidation-reduction potentials. | the four-residue reverse turn -met56-gly-asp-glu59- in the clostridium beijerinckii flavodoxin provides the majority of the critical interactions with the isoalloxazine ring of the flavin mononucleotide (fmn) cofactor that contribute to the binding and the differential stabilization of its three redox states. direct side chain contacts include the sulfur-ring interaction of met56, which primarily influences the oxidized and hydroquinone states, and the hydrogen bond by glu59 with the n3h, which ... | 2001 | 11695902 |
| continuous production of isopropanol and butanol using clostridium beijerinckii dsm 6423. | clostridium beijerinckii dsm 6423 was studied using different continuous production methods to give maximum and stable production of isopropanol and n-butanol. in a single-stage continuous culture, when wood pulp was added as a cell holding material, we could increase the solvent productivity from 0.47 to 5.52 g l(-1) h(-1) with the yield of 54% from glucose. the overall solvent concentration of 7.51 g l(-1) (39.4% isopropanol and 60.6% n-butanol) with the maximum solvent productivity of 0.84 g ... | 2011 | 21573939 |
| nutritional requirements of solventogenic clostridia growing under butanol stress and co-utilization of d-glucose/d-xylose. a meta-analysis and a functional validation. | recent advances in systems biology, omics, and computational studies allow us to carry out data mining for improving biofuel production bioprocesses. of particular interest are bioprocesses that center on microbial capabilities to biotransform both the hexose and the pentose fractions present in crop residues. this called for a systematic exploration of media components to obtain higher density cultures and more productive fermentation operations than are currently found. by using a meta-analysi ... | 2011 | 21602379 |
| clostridium beijerinckii mutant with high inhibitor tolerance obtained by low-energy ion implantation. | clostridium beijerinckii mutant strain ib4, which has a high level of inhibitor tolerance, was screened by low-energy ion implantation and used for butanol fermentation from a non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (sahhc). evaluation of toxicity showed c. beijerinckii ib4 had a higher level of tolerance than parent strain c. beijerinckii ncimb 8052 for five out of six phenolic compounds tested (the exception was vanillin). using glucose as carb ... | 2011 | 21789489 |
| butanol production from crystalline cellulose by co-cultured clostridium thermocellum and clostridium saccharoperbutylacetonicum n1-4. | we investigated butanol production from crystalline cellulose by co-cultured cellulolytic clostridium thermocellum and the butanol-producing strain, c. saccharoperbutylacetonicum (strain n1-4). butanol was produced from avicel cellulose after incubation with c. thermocellum for at least 24 h at 60°c before adding strain n1-4. butanol produced by strain n1-4 on 4% avicel cellulose peaked (7.9 g/l) after 9 days of incubation at 30°c and acetone was undetectable in this co-culture system. less buta ... | 2011 | 21764954 |
| clostridium beijerinckii mutant obtained by atmospheric pressure glow discharge producing high proportions of butanol and solvent yields. | with 30-ág glucose/l as carbon source, clostridium beijerinckii art124, a mutant created by atmospheric pressure glow discharge, produced 13.7-ág total solvent/l (containing 3.1-ág acetone/l, 10.4-ág butanol/l and 0.2-ág ethanol/l) in 72-áh. the mutant could also use sucrose or xylose or a mixture of glucose/xylose/arabinose with nearly equal yields. | 2011 | 21833546 |
| degeneration of solventogenic clostridium strains monitored by fourier transform infrared spectroscopy of bacterial cells. | strain degeneration in solventogenic clostridia is a known problem in the technical acetone-butanol fermentation bioprocess, especially in the continuous process mode. clostridial strain degeneration was studied by fourier transform infrared (ft-ir) spectroscopy of the bacterial cells. degenerative variant formation in two strains, clostridium beijerinckii ncimb 8052 and clostridium species aa332, was detected spectroscopically. colonies on solid media were sampled, or assayed directly in situ b ... | 2001 | 11781807 |
| outgrowth inhibition of clostridium beijerinckii spores by a bacteriocin-producing lactic culture in ovine milk cheese. | in the manufacture of model cheeses, ovine milk was deliberately contaminated with spores of clostridium beijerinckii inia 63, a wild isolate from manchego cheese with late blowing defect, and inoculated with nisin- and lacticin 481-producing lactococcus lactis subsp. lactis inia 415 as starter, to test its potential to prevent the late blowing defect, or with l. lactis subsp. lactis inia 415-2, a spontaneous mutant not producing bacteriocins. cheeses made individually with the lactococcal strai ... | 2011 | 21849216 |
| butanol production by clostridium beijerinckii ba101 in an immobilized cell biofilm reactor: increase in sugar utilization. | acetone butanol ethanol was produced in a continuous immobilized cell (biofilm) plug-flow reactor inoculated with clostridium beijerinckii ba101. to achieve high reactor productivity, c. beijerinckii ba101 cells were immobilized by adsorption onto clay brick. the continuous plug-flow reactor offers high productivities owing to reduced butanol inhibition and increased cell concentration. although high productivity was achieved, it was at the expense of low sugar utilization (30.3%). to increase s ... | 2002 | 12018285 |
| anthrahydroquinone-2,6,-disulfonate (ah2qds) increases hydrogen molar yield and xylose utilization in growing cultures of clostridium beijerinckii. | h(2) production and xylose utilization were investigated using the fermentative culture clostridium beijerinckii ncimb 8052. adding anthrahydroquinone-2,6-disulfonate (ah(2)qds) increased the extent of xylose utilization by 56% and hydrogen molar yield by 24-37%. enhanced hydrogen molar yield correlated with increased xylose utilization and increases in the acetate/butyrate product ratio. an electron balance indicated that ah(2)qds shifted the electrons from the butyric acid pathway (nadh-depend ... | 2011 | 21947605 |
| production of butanol from starch-based waste packing peanuts and agricultural waste. | we examined the fermentation of starch-based packing peanuts and agricultural wastes as a source of fermentable carbohydrates using clostridium beijerinckii ba101. using semidefined p2 medium containing packing peanuts and agricultural wastes, instead of glucose as a carbohydrate source, we measured characteristics of the fermentation including solvent production, productivity, and yield. with starch as substrate (control), the culture produced 24.7 g l(-1) acetone-butanol-ethanol (abe), while w ... | 2002 | 12242632 |
| structural basis for the enhanced thermal stability of alcohol dehydrogenase mutants from the mesophilic bacterium clostridium beijerinckii: contribution of salt bridging. | previous research in our laboratory comparing the three-dimensional structural elements of two highly homologous alcohol dehydrogenases, one from the mesophile clostridium beijerinckii (cbadh) and the other from the extreme thermophile thermoanaerobacter brockii (tbadh), suggested that in the thermophilic enzyme, an extra intrasubunit ion pair (glu224-lys254) and a short ion-pair network (lys257-asp237-arg304-glu165) at the intersubunit interface might contribute to the extreme thermal stability ... | 2002 | 12381840 |
| effects of nisin and reutericyclin on resistance of endospores of clostridium spp. to heat and high pressure. | the effects of high pressure, temperature, and antimicrobial compounds on endospores of clostridium spp. were examined. minimal inhibitory concentrations (mic) of nisin and reutericyclin were determined for vegetative cells and endospores of clostridium sporogenes atcc 7955, clostridium beijerinckii atcc 8260, and clostridium difficile 3195. endospores of c. sporogenes atcc 7955 and c. beijerinckii atcc 8260 were exposed to 90 °c and 90 °c/600 mpa in the presence of 16 mg l(-1) nisin or 6.4 mg l ... | 2012 | 23498177 |
| butanol-ethanol dehydrogenase and butanol-ethanol-isopropanol dehydrogenase: different alcohol dehydrogenases in two strains of clostridium beijerinckii (clostridium butylicum). | alcohol-producing strains of clostridium beijerinckii (clostridium butylicum) produce, besides acetone, either n-butanol and ethanol or n-butanol, ethanol, and isopropanol as their characteristic products. alcohol dehydrogenase has been isolated from a strain (nrrl b593) of c. beijerinckii producing isopropanol and from a strain (nrrl b592) not producing isopropanol. butanol-ethanol dehydrogenase activities were present in both strains, but isopropanol dehydrogenase activity was present only in ... | 1987 | 16347317 |
| experience with solvent production by clostridium beijerinckii in continuous culture. | | 1983 | 18548731 |
| interactions between bacillus cereus cgmcc 1.895 and clostridium beijerinckii ncimb 8052 in coculture for butanol production under nonanaerobic conditions. | low oxygen tolerance and substrate restriction continues to hamper the process of biobutanol industrialization. in this work, butanol fermentation with cocultures of bacillus cereus china general microbiological culture collection center (cgmcc) 1.895 and clostridium beijerinckii ncimb 8052 under nonanaerobic conditions was investigated, and the interactions between these two strains were examined. the addition of b. cereus cgmcc 1.895 resulted in higher oxygen tolerance and a wider range of sub ... | 2016 | 27306691 |
| the antimicrobial action of chitosan, low molar mass chitosan, and chitooligosaccharides on human colonic bacteria. | antibacterial effect of chitooligosaccharides (cos) and low molar mass chitosans (lmwc) is considered as one of the most important characteristics of chitosan (cs) hydrolysates. here, we show the in vitro effect of different cos, lmwc, and cs on representative anaerobic bacteria isolated from human colon as a possibility of targeting modification of colonic microflora composition by supplementation of dietary cs products by humans. specific growth rate of seven selected nonpathogenic anaerobic b ... | 2012 | 22528310 |
| a roadmap for gene system development in clostridium. | clostridium species are both heroes and villains. some cause serious human and animal diseases, those present in the gut microbiota generally contribute to health and wellbeing, while others represent useful industrial chassis for the production of chemicals and fuels. to understand, counter or exploit, there is a fundamental requirement for effective systems that may be used for directed or random genome modifications. we have formulated a simple roadmap whereby the necessary gene systems maybe ... | 2016 | 27234263 |
| chitinolytic activities of clostridium sp. jm2 isolated from stool of human administered per orally by chitosan. | the novel chitinolytic bacterium clostridium beijerinckii strain jm2 was isolated from the stool of healthy volunteers supplied daily per orally with 3 g of chitosan. the bacterium grown on colloidal chitin produced a complete array of chitinolytic enzymes. significant activities of endochitinase, exochitinase and chitosanase were excreted into the medium (301, 282 and 268 nkat/microg protein, respectively). the high cellular activity of n-acetyl-beta-glucosaminidase (nagase) and chitosanase wer ... | 2008 | 18661303 |
| enhanced butanol production by clostridium beijerinckii ba101 grown in semidefined p2 medium containing 6 percent maltodextrin or glucose. | dramatically elevated levels of butanol and acetone resulted in higher butanol and total solvent yields for hyperamylolytic clostridium beijerinckii ba101 relative to the ncimb 8052 parent strain grown in semidefined p2 medium containing either 6% glucose or star-dri 5 maltodextrin. c. beijerinckii ba101 consistently produced on the order of 19 g of butanol per liter in 20-liter batch fermentations. this represents a greater than 100% increase in butanol concentration by the ba101 strain compare ... | 1997 | 16535628 |
| characterisation of a transposon-induced pleiotropic mutant of clostridium acetobutylicum p262. | transposon-induced metronidazole resistance was used as a selection system for the isolation of clostridium acetobutylicum p262 mutants with altered electron transport pathways. the metronidazole resistant transconjugant of interest, mutant 3r, displayed resistance to dna damaging agents, uv and bleomycin, and harboured a single transposon insertion within a structural gene, designated sum(susceptibility to metronidazole). the sum gene encoded a 334 amino-acid protein, with 36% identity and 57-5 ... | 1997 | 16887617 |
| characterization of the sol operon in butanol-hyperproducing clostridium saccharoperbutylacetonicum strain n1-4 and its degeneration mechanism. | the solventogenic sol operon consisting of bld, ctfa, ctfb, and adc was cloned from clostridium saccharoperbutylacetonicum strain n1-4. these genes share as high as 95-98% similarity with the corresponding sol genes of clostridium beijerinckii ncimb 8052. the n1-4 sol gene cluster was transcribed in a polycistronic manner under the control of two promoters, and its transcription was highly induced during solventogenesis. strain dgn3-4, the degenerated strain derived from n1-4, maintained the sol ... | 2007 | 17213660 |
| acetone-butanol-ethanol production from substandard and surplus dates by egyptian native clostridium strains. | one hundred and seven mesophilic isolates of clostridium were isolated from agricultural soils cultivated with different plants in assuit governorate, egypt. eighty isolates (out of 107) showed the ability to produce abe (acetone, butanol and ethanol) on t6 medium ranging from 0.036 to 31.89 g/l. the highest numbers of abe producing isolates were obtained from soil samples of potato contributing 27 isolates, followed by 18 isolates from wheat and 10 isolates from onion. on the other hand, there ... | 2015 | 25557787 |
| development of a triplex real-time pcr assay for the simultaneous detection of clostridium beijerinckii, clostridium sporogenes and clostridium tyrobutyricum in milk. | clostridium beijerinckii, clostridium sporogenes and clostridium tyrobutyricum are considered the leading bacteria implicated in late blowing defects affecting semi-hard and hard cheese production. the aim of this study was to develop a multiplex real-time pcr (qpcr) analysis for a rapid and simultaneous detection of c. beijerinckii, c. sporogenes and c. tyrobutyricum, using specific primers respectively targeting the nifh, geraa and enr genes. the limits of detection in raw milk were 300 cfu/50 ... | 2015 | 25870135 |
| use of the fluorescent probe laurdan to label and measure inner membrane fluidity of endospores of clostridium spp. | a method for measuring the fluidity of inner membranes of populations of endospores of clostridium spp. with a fluorescent dye was developed. cells of clostridium beijerinckii atcc 8260 and clostridium sporogenes atcc 7955 were allowed to sporulate in the presence of 6-dodecanoyl-2-dimethylaminonaphthalene (laurdan) on a soil-based media. labeling of endospores with laurdan did not affect endospore viability. removal of the outer membranes of endospores was done using a chemical treatment and co ... | 2012 | 22884687 |
| the clostron: mutagenesis in clostridium refined and streamlined. | the recent development of the clostron group ii intron directed mutagenesis tool for clostridium has advanced genetics in this genus, and here we present several significant improvements. we have shown how marker re-cycling can be used to construct strains with multiple mutations, demonstrated using flp/frt in clostridium acetobutylicum; tested the capacity of the system for the delivery of transgenes to the chromosome of clostridium sporogenes, which proved feasible for 1.0kbp transgenes in add ... | 2010 | 19891996 |
| butanol production from agricultural residues: impact of degradation products on clostridium beijerinckii growth and butanol fermentation. | during pretreatment and hydrolysis of fiber-rich agricultural biomass, compounds such as salts, furfural, hydroxymethyl furfural (hmf), acetic, ferulic, glucuronic, rho-coumaric acids, and phenolic compounds are produced. clostridium beijerinckii ba101 can utilize the individual sugars present in lignocellulosic [e.g., corn fiber, distillers dry grain solubles (ddgs), etc] hydrolysates such as cellobiose, glucose, mannose, arabinose, and xylose. in these studies we investigated the effect of som ... | 2007 | 17274071 |
| comparison of different mixed cultures for bio-hydrogen production from ground wheat starch by combined dark and light fermentation. | composition of the mixed culture was varied in combined dark-light fermentation of wheat powder starch in order to improve hydrogen gas formation rate and yield. heat-treated anaerobic sludge and pure culture of clostridium beijerinckii (dsmz 791t) were combined with two different light fermentation bacteria of rhodobacter sphaeroides (rs-nrrl and rs-rv) in order to select a more suitable mixture resulting in high hydrogen yield and formation rate. a combination of the anaerobic sludge and rs-nr ... | 2010 | 20033469 |
| construction of heterologous gene expression cassettes for the development of recombinant clostridium beijerinckii. | gene-expression cassettes for the construction of recombinant clostridium beijerinckii were developed as potential tools for metabolic engineering of c. beijerinckii. gene expression cassettes containing cole1 origin and pamb origin along with the erythromycin resistance gene were constructed, in which promoters from escherichia coli, lactococcus lactis, ralstonia eutropha, c. acetobutylicum, and c. beijerinckii are examined as potential promoters in c. beijerinckii. zymogram analysis of the cel ... | 2016 | 26780375 |
| optimized transformation of newly constructed escherichia coli-clostridia shuttle vectors into clostridium beijerinckii. | three escherichia coli-clostridia shuttle vectors, pkba411-mcs, pkbe411-mcs, and pkbm411-mcs, which contain p15a, cole1, and pmb1 origins for replication in e. coli, respectively, along with the pamb origin for replication in c. beijerinckii, were constructed and examined for their transformation efficiencies into clostridium beijerinckii ncimb8052. the transformation condition of pkbm411-mcs, which was optimized by varying resistance, buffer composition, and dna concentration, was further emplo ... | 2015 | 26152821 |
| identification, purification and characterization of furfural transforming enzymes from clostridium beijerinckii ncimb 8052. | generation of microbial inhibitory compounds such as furfural and 5-hydroxymethylfurfural (hmf) is a formidable roadblock to fermentation of lignocellulose-derived sugars to butanol. bioabatement offers a cost effective strategy to circumvent this challenge. although clostridium beijerinckii ncimb 8052 can transform 2-3 g/l of furfural and hmf to their less toxic alcohols, higher concentrations present in biomass hydrolysates are intractable to microbial transformation. to delineate the mechanis ... | 2015 | 25796546 |
| butanol production by clostridium beijerinckii atcc 55025 from wheat bran. | wheat bran, a by-product of the wheat milling industry, consists mainly of hemicellulose, starch and protein. in this study, the hydrolysate of wheat bran pretreated with dilute sulfuric acid was used as a substrate to produce abe (acetone, butanol and ethanol) using clostridium beijerinckii atcc 55025. the wheat bran hydrolysate contained 53.1 g/l total reducing sugars, including 21.3 g/l of glucose, 17.4 g/l of xylose and 10.6 g/l of arabinose. c. beijerinckii atcc 55025 can utilize hexose and ... | 2010 | 20393827 |
| clostridium chromiireducens sp. nov., a novel strain isolated from a cr(vi)-contaminated soil. | strain gcaf-1 is a cr(vi)-resistant, gram positive, spore-forming obligate anaerobe that was 2 isolated from a chromium-contaminated soil by its ability to reduce cr(vi) in low 3 concentrations, fe(iii), and the humic-acid analog aqds. a mixed acid fermentation resulting 4 in accumulation of acetate, butyrate and lactate is observed during growth of gcaf-1 on 5 glucose. morphological studies on this strain indicate the presence of peritrichous flagella, pili 6 and an s-layer. major cellular fatt ... | 2010 | 21148674 |
| large number of phosphotransferase genes in the clostridium beijerinckii ncimb 8052 genome and the study on their evolution. | clostridium beijerinckii is a valuable bacteria species which has the ability of abe (acetone, butanol and ethanol) production. it has been shown that phosphotransferase (pts) is an important and common system for both carbohydrate uptake and phosphorylation in bacteria, but detailed study of the system, especially its fructose/mannose/sorbose family is scant. | 2010 | 21172059 |
| development of agave as a dedicated biomass source: production of biofuels from whole plants. | agave species can grow well in semi-arid marginal agricultural lands around the world. selected agave species are used largely for alcoholic beverage production in mexico. there are expanding research efforts to use the plentiful residues (bagasse) for ethanol production as the beverage manufacturing process only uses the juice from the central core of mature plants. here, we investigate the potential of over a dozen agave species, including three from cold semi-arid regions of the united states ... | 2015 | 26056533 |
| bioproduction of butanol in bioreactors: new insights from simultaneous in situ butanol recovery to eliminate product toxicity. | simultaneous acetone butanol ethanol (abe) fermentation by clostridium beijerinckii p260 and in situ product recovery was investigated using a vacuum process operated in two modes: continuous and intermittent. integrated batch fermentations and abe recovery were conducted at 37 °c using a 14-l bioreactor (7.0 l fermentation volume) containing initial substrate (glucose) concentration of 60 g/l. the bioreactor was connected in series with a condensation system and vacuum pump. vacuum was applied ... | 2011 | 21370232 |
| gene transcription repression in clostridium beijerinckii using crispr-dcas9. | crispr-cas9 has been explored as a powerful tool for genome engineering for many organisms. meanwhile, dcas9 which lacks endonuclease activity but can still bind to target loci has been engineered for efficient gene transcription repression. clostridium beijerinckii, an industrially significant species capable of biosolvent production, is generally difficult to metabolically engineer. recently, we reported our work in developing customized crispr-cas9 system for genome engineering in c. beijerin ... | 2016 | 27240718 |
| crispr-based genome editing and expression control systems in clostridium acetobutylicum and clostridium beijerinckii. | solventogenic clostridia are important industrial microorganisms that produce various chemicals and fuels. effective genetic tools would facilitate physiological studies aimed both at improving our understanding of metabolism and optimizing solvent productivity through metabolic engineering. here we have developed an all-in-one, crispr-based genome editing plasmid, pnickclos, that can be used to achieve successive rounds of gene editing in clostridium acetobutylicum atcc 824 and clostridium beij ... | 2016 | 27213844 |
| bacterial genome editing with crispr-cas9: deletion, integration, single nucleotide modification, and desirable "clean" mutant selection in clostridium beijerinckii as an example. | crispr-cas9 has been demonstrated as a transformative genome engineering tool for many eukaryotic organisms; however, its utilization in bacteria remains limited and ineffective. here we explored streptococcus pyogenes crispr-cas9 for genome editing in clostridium beijerinckii (industrially significant but notorious for being difficult to metabolically engineer) as a representative attempt to explore crispr-cas9 for genome editing in microorganisms that previously lacked sufficient genetic tools ... | 2016 | 27115041 |
| hydrogen production by the newly isolated clostridium beijerinckii rzf-1108. | a fermentative hydrogen-producing strain, rzf-1108, was isolated from a biohydrogen reactor, and identified as clostridium beijerinckii on the basis of the 16s rrna gene analysis and physiobiochemical characteristics. the effects of culture conditions on hydrogen production by c. beijerinckii rzf-1108 were investigated in batch cultures. the hydrogen production and growth of strain rzf-1108 were highly dependent on temperature, initial ph and substrate concentration. yeast extract was a favorabl ... | 2011 | 21421301 |
| improved efficiency of separate hexose and pentose fermentation from steam-exploded corn stalk for butanol production using clostridium beijerinckii. | water extract of steam-exploded corn stalk (secs) was detoxified and used as feed for acetone-butanol-ethanol (abe) fermentation using clostridium beijerinckii. utilization of water extract improved the total abe yield (g abe/g dry secs). separated fermentation showed higher fermentability (0.078 g abe/g dry secs) over typical fermentation (0.058 g abe/g dry secs). furthermore, the final abe yields (g abe/g utilized sugar) from water extract neutralized by ca(oh)(2), naoh, and na(2)so(3) were 0. ... | 2011 | 21424838 |
| butanol production from the effluent of hydrogen fermentation. | the purpose of the study was to recover butanol from the effluent of the hydrogen-producing bioreactor containing acetate, butyrate, and carbohydrate. the butanol production by clostridium beijerinckii nrrl b592 was evaluated under both unsterilized and sterilized conditions for examining the potential of butanol production for the practical application. sucrose of 10 g/l and butyrate of 2 g/l coupled with acetate buffer were used to mimic the effluent. sucrose was completely consumed in the bot ... | 2011 | 21436562 |
| acetone production in solventogenic clostridium species: new insights from non-enzymatic decarboxylation of acetoacetate. | development of a butanologenic strain with high selectivity for butanol production is often proposed as a possible route for improving the economics of biobutanol production by solventogenic clostridium species. the acetoacetate decarboxylase (aadc) gene encoding acetoacetate decarboxylase (aadc), which catalyzes the decarboxylation of acetoacetate into acetone and co(2), was successfully disrupted by homologous recombination in solventogenic clostridium beijerinckii ncimb 8052 to generate an aa ... | 2011 | 21533580 |
| influence of reduced electron shuttling compounds on biological h2 production in the fermentative pure culture clostridium beijerinckii. | several reports suggest that extracellular electron shuttles influence fermentative metabolism in a beneficial manner for bioremediation and biotechnology strategies. the focus of this research was to characterize the effects of reduced electron shuttling molecules on fermentative h(2) production. reduced electron shuttles may provide reducing equivalents to generate h(2), which influences alternate cellular processes. electron shuttling compounds cycle between reduced-oxidized states and influe ... | 2008 | 18167025 |
| redox potential difference between desulfovibrio vulgaris and clostridium beijerinckii flavodoxins. | the redox potential of the flavin mononucleotide (fmn) hydroquinones for one-electron reduction in the desulfovibrio vulgaris ( d. vulgaris) flavodoxin ( e sq/hq for fmnh (*)/fmnh (-)) was calculated using the crystal structure of the relevant hydroquinone form and compared to the results of the clostridium beijerinckii ( c. beijerinckii) flavodoxin. in d. vulgaris and c. beijerinckii flavodoxins, the protein side chain causes significant downshifts of 170 and 240 mv in e sq/hq, respectively. in ... | 2008 | 18355044 |
| continuos ibe fermentation by immobilized growing clostridium beijerinckii cells in a stirred-tank fermentor. | the potential of continuous isopropanol-butanol-ethanol (ibe) fermentation by ca-alginate-immobilized clostridium beijerinckii cells in a continuous stirred-tank reactor is investigated. a mathematical model is presented to describe steady-state reactor performance. it appeared to be possible to use the biocatalyst particles repeatedly for successive fermentations (at least three times for a total duration of two months). reactor productivity was 6-16 times higher than that of a batch fermentati ... | 1983 | 18548553 |
| transcriptional analysis of clostridium beijerinckii ncimb 8052 to elucidate role of furfural stress during acetone butanol ethanol fermentation. | furfural is the prevalent microbial inhibitor generated during pretreatment and hydrolysis of lignocellulose biomass to monomeric sugars, but the response of acetone butanol ethanol (abe) producing clostridium beijerinckii ncimb 8052 to this compound at the molecular level is unknown. to discern the effect of furfural on c. beijerinckii and to gain insight into molecular mechanisms of action and detoxification, physiological changes of furfural-stressed cultures during acetone butanol ethanol (a ... | 2013 | 23642190 |
| lipid diversity among botulinum neurotoxin-producing clostridia. | clostridium botulinum has been classified into four groupings (groups i to iv) based on physiological characteristics and 16s rrna sequencing. we have examined the lipid compositions of 11 representative strains of c. botulinum and a strain of clostridium sporogenes by 2d-tlc and by ms. all strains contained phosphatidylglycerol (pg), cardiolipin (cl) and phosphatidylethanolamine (pe) in both the all-acyl and the alk-1'-enyl (plasmalogen) forms. five strains in proteolytic group i, which are rel ... | 2012 | 22837302 |
| genomic approach to studying nutritional requirements of clostridium tyrobutyricum and other clostridia causing late blowing defects. | clostridium tyrobutyricum is the main microorganism responsible for the late blowing defect in hard and semi-hard cheeses, causing considerable economic losses to the cheese industry. deeper knowledge of the metabolic requirements of this microorganism can lead to the development of more effective control approaches. in this work, the amino acids and b vitamins essential for sustaining the growth of c. tyrobutyricum were investigated using a genomic approach. as the first step, the genomes of fo ... | 2016 | 27375262 |
| seasonal occurrence and molecular diversity of clostridia species spores along cheesemaking streams of 5 commercial dairy plants. | five commercial dairy plants were monitored over a 17-mo period to determine the seasonal occurrence of clostridium spores in streams from the cheesemaking process. every 2 mo, samples of raw milk (rm), separated cream (sc), pasteurized and standardized vat milk (psvm), psvm + lysozyme (psvm+l), and manufactured cheese aged for 60 to 90 d were processed for analysis. molecular diversity of the main species identified was determined using repetitive element palindromic pcr. the mean anaerobic spo ... | 2016 | 26923043 |
| impact of organic loading rate on biohydrogen production in an up-flow anaerobic packed bed reactor (uanpbr). | this study assesses the impact of organic loading rate on biohydrogen production from glucose in an up-flow anaerobic packed bed reactor (uanpbr). two mesophilic uapbrs (uanpbr1 and 2) were tested at organic loading rates (olrs) ranging from 6.5 to 51.4 g cod l(-1)d(-1). to overcome biomass washout, design modifications were made in the uanpbr2 to include a settling zone to capture the detached biomass. the design modifications in uanpbr2 increased the average hydrogen yield from 0.98 to 2.0 mol ... | 2014 | 24865326 |
| molecular characterization of an nadph-dependent acetoin reductase/2,3-butanediol dehydrogenase from clostridium beijerinckii ncimb 8052. | acetoin reductase is an important enzyme for the fermentative production of 2,3-butanediol, a chemical compound with a very broad industrial use. here, we report on the discovery and characterization of an acetoin reductase from clostridium beijerinckii ncimb 8052. an in silico screen of the c. beijerinckii genome revealed eight potential acetoin reductases. one of them (cbei_1464) showed substantial acetoin reductase activity after expression in escherichia coli. the purified enzyme (c. beijeri ... | 2014 | 24441158 |
| Metabolic Engineering of Clostridium acetobutylicum ATCC 824 for Isopropanol-Butanol-Ethanol Fermentation. | Clostridium acetobutylicum naturally produces acetone as well as butanol and ethanol. Since acetone cannot be used as a biofuel, its production needs to be minimized or suppressed by cell or bioreactor engineering. Thus, there have been attempts to disrupt or inactivate the acetone formation pathway. Here we present another approach, namely converting acetone to isopropanol by metabolic engineering. Since isopropanol can be used as a fuel additive, the mixture of isopropanol, butanol, and ethano ... | 2011 | 22210214 |
| single-nucleotide resolution analysis of the transcriptome structure of clostridium beijerinckii ncimb 8052 using rna-seq. | clostridium beijerinckii is an important solvent producing microorganism. the genome of c. beijerinckii ncimb 8052 has recently been sequenced. although transcriptome structure is important in order to reveal the functional and regulatory architecture of the genome, the physical structure of transcriptome for this strain, such as the operon linkages and transcript boundaries are not well understood. | 2011 | 21962126 |
| metabolic network reconstruction and genome-scale model of butanol-producing strain clostridium beijerinckii ncimb 8052. | solventogenic clostridia offer a sustainable alternative to petroleum-based production of butanol--an important chemical feedstock and potential fuel additive or replacement. c. beijerinckii is an attractive microorganism for strain design to improve butanol production because it (i) naturally produces the highest recorded butanol concentrations as a byproduct of fermentation; and (ii) can co-ferment pentose and hexose sugars (the primary products from lignocellulosic hydrolysis). interrogating ... | 2011 | 21846360 |
| Optimization of butanol production from tropical maize stalk juice by fermentation with Clostridium beijerinckii NCIMB 8052. | Mixed sugars from tropical maize stalk juice were used to carry out butanol fermentation with Clostridium beijerinckii NCIMB 8052. Batch experiments employing central composite design (CCD) and response surface methodology (RSM) optimization were performed to evaluate effects of three factors, i.e. pH, initial total sugar concentration, and agitation rate on butanol production. Optimum conditions of pH 6.7, sugar concentration 42.2g/L and agitation rate 48 rpm were predicted, under which a maxim ... | 2011 | 21893411 |
| use of proteomic analysis to elucidate the role of calcium in acetone-butanol-ethanol fermentation by clostridium beijerinckii ncimb 8052. | calcium carbonate increases growth, substrate utilization, and acetone-butanol-ethanol (abe) fermentation by clostridium beijerinckii ncimb 8052. toward an understanding of the basis for these pleiotropic effects, we profiled changes in the c. beijerinckii ncimb 8052 proteome that occur in response to the addition of caco(3). we observed increases in the levels of different heat shock proteins (grpe and dnak), sugar transporters, and proteins involved in dna synthesis, repair, recombination, and ... | 2013 | 23104411 |
| novel clostridial fusants in comparison with co-cultured counterpart species for enhanced production of biobutanol using green renewable and sustainable feedstock. | in this work, biobutanol was produced through simultaneous saccharification and fermentation (ssf) of wheat straw (ws) that traditionally produces acetone, butanol and ethanol solvents (abe). thermal stability was imparted to two mesophilic clostridial wild strains (clostridium beijerinckii and clostridium acetobutylicum) through protoplast fusion with that of a corresponding thermophilic clostridial species (clostridium thermocellum). production was pursued by the fused strains at 45 °c compare ... | 2015 | 26395807 |
| use of cupriavidus basilensis-aided bioabatement to enhance fermentation of acid-pretreated biomass hydrolysates by clostridium beijerinckii. | lignocellulose-derived microbial inhibitors (ldmics) prevent efficient fermentation of miscanthus giganteus (mg) hydrolysates to fuels and chemicals. to address this problem, we explored detoxification of pretreated mg biomass by cupriavidus basilensis atcc(®)baa-699 prior to enzymatic saccharification. we document three key findings from our test of this strategy to alleviate ldmic-mediated toxicity on clostridium beijerinckii ncimb 8052 during fermentation of mg hydrolysates. first, we demonst ... | 2016 | 27400988 |
| production of 1,3-propanediol by clostridium beijerinckii dsm 791 from crude glycerol and corn steep liquor: process optimization and metabolic engineering. | 1,3-propanediol (1,3-pdo) production from crude glycerol, a byproduct from biodiesel manufacturing, by clostridium beijerinckii dsm 791 was studied with corn steep liquor as an inexpensive nitrogen source replacing yeast extract in the fermentation medium. a stable, long-term 1,3-pdo production from glycerol was demonstrated with cells immobilized in a fibrous bed bioreactor operated in a repeated batch mode, which partially circumvented the 1,3-pdo inhibition problem. the strain was then engine ... | 2016 | 27085150 |
| identification of a glucose-mannose phosphotransferase system in clostridium beijerinckii. | effective uptake of fermentable substrates is a fundamentally important aspect of any fermentation process. the solventogenic bacterium clostridium beijerinckii is noted for its ability to ferment a wide range of carbohydrates, yet few of its sugar transport systems have been characterized. in common with other anaerobes, c. beijerinckii shows a marked dependence on the pep-dependent phosphotransferase system (pts) for sugar accumulation. in this study, the gene cbe0751 encoding the sugar-specif ... | 2016 | 26940293 |
| engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light. | production of alternate fuels or chemicals directly from solar energy and carbon dioxide using engineered cyanobacteria is an attractive method to reduce petroleum dependency and minimize carbon emissions. here, we constructed a synthetic pathway composed of acetyl-coa acetyl transferase (encoded by thl), acetoacetyl-coa transferase (encoded by atoad), acetoacetate decarboxylase (encoded by adc) and secondary alcohol dehydrogenase (encoded by adh) in synechococcus elongatus strain pcc 7942 to pr ... | 2013 | 24076145 |
| dual substrate specificity of an n-acetylglucosamine phosphotransferase system in clostridium beijerinckii. | the solventogenic clostridia have a considerable capacity to ferment carbohydrate substrates with the production of acetone and butanol, making them attractive organisms for the conversion of waste materials to valuable products. in common with other anaerobes, the clostridia show a marked dependence on the phosphoenolpyruvate (pep)-dependent phosphotransferase system (pts) to accumulate sugars and sugar derivatives. in this study, we demonstrate that extracts of clostridium beijerinckii grown o ... | 2013 | 23995920 |
| metabolic engineering of candida utilis for isopropanol production. | a genetically-engineered strain of the yeast candida utilis harboring genes encoding (1) an acetoacetyl-coa transferase from clostridium acetobutylicum atcc 824, (2) an acetoacetate decarboxylase, and (3) a primary-secondary alcohol dehydrogenase derived from clostridium beijerinckii nrrl b593 produced up to 0.21 g/l of isopropanol. because the engineered strain accumulated acetate, isopropanol titer was improved to 1.2 g/l under neutralized fermentation conditions. optimization of isopropanol p ... | 2013 | 23674152 |
| efficient acetone-butanol-ethanol production by clostridium beijerinckii from sugar beet pulp. | sugar beet pulp (sbp) has been investigated as a promising feedstock for abe fermentation by clostridium beijerinckii. although lignin content in sbp is low, a pretreatment is needed to enhance enzymatic hydrolysis and fermentation yields. autohydrolysis at ph 4 has been selected as the best pretreatment for sbp in terms of sugars release and acetone and butanol production. the best overall sugars release yields from raw sbp ranged from 66.2% to 70.6% for this pretreatment. the highest abe yield ... | 2015 | 25965949 |
| isolation, characterization and evaluation of hyper 2-propanol producing bacteria from singapore environment. | three hyper 2-propanol producing strains were isolated from singapore environment using an enrichment step and a high through-put screening step. the analysis of the amplified 16s rdna revealed that the isolates belonged to clostridium species and they were named as clostridium sp. bt10-1, clostridium sp. m10-1 and clostridium sp. pu31-4. at 1 l scale, the 2-propanol titer of these positive strains was 1.6-2.1 times of that of clostridium beijerinckii nrrl b593, which is so far the most efficien ... | 2013 | 23361972 |
| biosynthesis of the fatty acid isopropyl esters by engineered escherichia coli. | the fatty acid methyl esters and fatty acid ethyl esters are known as biodiesels which are considered to be renewable, nontoxic and biodegradable biofuels. however, the conventional biodiesels show a high crystallization temperature which is one of the most critical obstacles against the widespread biodiesel usage. the high crystallization temperature of biodiesel can be reduced by replacing the methyl or ethyl ester with an isopropyl moiety. here we report on a strategy to establish biosynthesi ... | 2017 | 28465060 |
| butanol fermentation of the brown seaweed laminaria digitata by clostridium beijerinckii dsm-6422. | seaweed represents an abundant, renewable, and fast-growing biomass resource for 3rd generation biofuel production. this study reports an efficient butanol fermentation process carried out by clostridium beijerinckii dsm-6422 using enzymatic hydrolysate of the sugar-rich brown seaweed laminaria digitata harvested from the coast of the danish north sea as substrate. the highest butanol yield (0.42g/g-consumed-substrates) compared to literature was achieved, with a significantly higher butanol:ace ... | 0 | 28432948 |
| effect of ozonolysis parameters on the inhibitory compound generation and on the production of ethanol by pichia stipitis and acetone-butanol-ethanol by clostridium from ozonated and water washed sugarcane bagasse. | sugarcane bagasse (scb) was ozone pretreated and detoxified by water washing, applying a l9(3)(4) orthogonal array (oa) design of experiments to study the effect of pretreatment parameters (moisture content, ozone concentration, ozone/oxygen flow and particle size) on the generation of inhibitory compounds and on the composition of hydrolysates of ozonated-washed samples. ozone concentration resulted the highest influence process parameter on delignification and sugar release after washing; whil ... | 2016 | 27428302 |
| enhanced robustness in acetone-butanol-ethanol fermentation with engineered clostridium beijerinckii overexpressing adhe2 and ctfab. | clostridium beijerinckii cc101 was engineered to overexpress aldehyde/alcohol dehydrogenase (adhe2) and coa-transferase (ctfab). solvent production and acid assimilation were compared between the parental and engineered strains expressing only adhe2 (cc101-sv4) and expressing adhe2, ald and ctfab (cc101-sv6). cc101-sv4 showed an early butanol production from glucose but stopped pre-maturely at a low butanol concentration of ∼6g/l. compared to cc101, cc101-sv6 produced more butanol (∼12g/l) from ... | 2017 | 28747008 |
| enhanced n-butanol production by clostridium beijerinckii mcmb 581 in presence of selected surfactant. | extractive butanol fermentation with non-ionic surfactant, a recently explored area, has shown promising results with several advantages but is relatively less investigated. this work reports the extractive fermentation with selected non-ionic surfactants (l62 and l62d) to enhance butanol production using a high-butanol producing strain (clostridium beijerinckii mcmb 581). biocompatibility studies with both the surfactants showed growth. higher concentrations of surfactant (>5%) affected the cel ... | 2017 | 28660448 |
| degradation of the fungal cell wall by clostridial strains isolated from soil subjected to biological soil disinfestation and biocontrol of fusarium wilt disease of spinach. | biological soil disinfestation (bsd) involves elimination of soil-borne plant pathogens in an environmentally friendly manner. two anaerobic bacterial strains (h110 and tb8) isolated from bsd-treated soil samples were analyzed for their roles in elimination of pathogenic fungi. the two strains were identified as clostridium beijerinckii based on 16s rrna gene sequences and phenotypic properties. the strains fermented various carbohydrates and produced acetate, butyrate, and n-butanol as major pr ... | 2017 | 28967048 |
| mono- and co-substrate utilization kinetics using mono- and co-culture of clostridium beijerinckii and clostridium saccharoperbutylacetonicum. | the effect of co-culturing c. beijerinckii and c. saccharoperbutylacetonicum for h2 production using mono- and co-substrates of glucose, starch, and cellulose was assessed. monod kinetic parameters (k, maximum specific substrate utilization rate; and ks, half-saturation constant) of the c. beijerinckii, c. saccharoperbutylacetonicum, and the co-culture were determined. co-cultures utilizing glucose competed for the substrate, but showed enhancement for utilizing starch. the maximum values for k ... | 2017 | 28554101 |
| development of an oxygen-independent flavin mononucleotide-based fluorescent reporter system in clostridium beijerinckii and its potential applications. | clostridium beijerinckii is a predominant solventogenic clostridia with great attraction for renewable liquid biofuel and biochemical production. metabolic engineering and synthetic biology can be employed to engineer the strain toward desirable phenotypes. however, current limited information such as promoter strength and gene regulation may hinder the efficient engineering of the strain. to investigate genetic information and complex cellular bioprocesses of c. beijerinckii, an in vivo fluores ... | 2018 | 29158189 |
| metabolic engineering of clostridium tyrobutyricum for n-butanol production: effects of coa transferase. | the overexpression of coa transferase (ctfab), which catalyzes the reaction: acetate/butyrate + acetoacetyl-coa → acetyl/butyryl-coa + acetoacetate, was studied for its effects on acid reassimilation and butanol biosynthesis in clostridium tyrobutyricum (δack, adhe2). the plasmid pmtl007 was used to co-express adhe2 and ctfab from clostridium acetobutylicum atcc 824. in addition, the sol operon containing ctfab, adc (acetoacetate decarboxylase), and ald (aldehyde dehydrogenase) was also cloned f ... | 2015 | 25851718 |