| butanol production by clostridium beijerinckii. part i: use of acid and enzyme hydrolyzed corn fiber. | fermentation of sulfuric acid treated corn fiber hydrolysate (sacfh) inhibited cell growth and butanol production (1.7+/-0.2g/l acetone butanol ethanol or abe) by clostridium beijerinckii ba101. treatment of sacfh with xad-4 resin removed some of the inhibitors resulting in the production of 9.3+/-0.5 g/l abe and a yield of 0.39+/-0.015. fermentation of enzyme treated corn fiber hydrolysate (etcfh) did not reveal any cell inhibition and resulted in the production of 8.6+/-1.0 g/l abe and used 24 ... | 2008 | 18061440 |
| the conserved glu-60 residue in thermoanaerobacter brockii alcohol dehydrogenase is not essential for catalysis. | glu-60 of the zinc-dependent thermoanaerobacter brockii alcohol dehydrogenase (tbadh) is a strictly conserved residue in all members of the alcohol dehydrogenase (adh) family. unlike most other adhs, the crystal structures of tbadh and its analogs, adh from clostridium beijerinckii (cbadh), exhibit a unique zinc coordination environment in which this conserved residue is directly coordinated to the catalytic zinc ion in the native form of the enzymes. to explore the role of glu-60 in tbadh catal ... | 2003 | 12592017 |
| redox potential difference between desulfovibrio vulgaris and clostridium beijerinckii flavodoxins. | the redox potential of the flavin mononucleotide (fmn) hydroquinones for one-electron reduction in the desulfovibrio vulgaris ( d. vulgaris) flavodoxin ( e sq/hq for fmnh (*)/fmnh (-)) was calculated using the crystal structure of the relevant hydroquinone form and compared to the results of the clostridium beijerinckii ( c. beijerinckii) flavodoxin. in d. vulgaris and c. beijerinckii flavodoxins, the protein side chain causes significant downshifts of 170 and 240 mv in e sq/hq, respectively. in ... | 2008 | 18355044 |
| continuos ibe fermentation by immobilized growing clostridium beijerinckii cells in a stirred-tank fermentor. | the potential of continuous isopropanol-butanol-ethanol (ibe) fermentation by ca-alginate-immobilized clostridium beijerinckii cells in a continuous stirred-tank reactor is investigated. a mathematical model is presented to describe steady-state reactor performance. it appeared to be possible to use the biocatalyst particles repeatedly for successive fermentations (at least three times for a total duration of two months). reactor productivity was 6-16 times higher than that of a batch fermentati ... | 1983 | 18548553 |
| identification and characterization of two novel clostridial bacteriocins, circularin a and closticin 574. | two novel antibacterial peptides of clostridial species were purified, n-terminally sequenced, and characterized. moreover, their structural genes were identified. closticin 574 is an 82-amino-acid bacteriocin produced by clostridium tyrobutyricum adriat 932. the supernatant of the producing strain showed a high level of activity against the indicator strain c. tyrobutyricum. the protein is synthesized as a preproprotein that is possibly secreted via the general secretion pathway, after which it ... | 2003 | 12620847 |
| crystalline alcohol dehydrogenases from the mesophilic bacterium clostridium beijerinckii and the thermophilic bacterium thermoanaerobium brockii: preparation, characterization and molecular symmetry. | two tetrameric nadp(+)-dependent bacterial secondary alcohol dehydrogenases have been crystallized in the apo- and the holo-enzyme forms. crystals of the holo-enzyme from the mesophilic clostridium beijerinckii (ncbad) belong to space group p2(1)2(1)2(1) with unit-cell dimensions a = 90.5, b = 127.9, c = 151.4 a. crystals of the apo-enzyme (cbad) belong to the same space group with unit-cell dimensions a = 80.4, b = 102.3, c = 193.5 a. crystals of the holo-enzyme from the thermophilic thermoanae ... | 1996 | 15299659 |
| transcriptional analysis of clostridium beijerinckii ncimb 8052 and the hyper-butanol-producing mutant ba101 during the shift from acidogenesis to solventogenesis. | clostridium beijerinckii is an anaerobic bacterium used for the fermentative production of acetone and butanol. the recent availability of genomic sequence information for c. beijerinckii ncimb 8052 has allowed for an examination of gene expression during the shift from acidogenesis to solventogenesis over the time course of a batch fermentation using a ca. 500-gene set dna microarray. the microarray was constructed using a collection of genes which are orthologs of members of gene families prev ... | 2008 | 18849451 |
| metabolic engineering of saccharomyces cerevisiae for the production of n-butanol. | abstract: background: increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol. results and conclusion: saccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (s. cerevisiae, escherichia coli, clostridium beijerinckii, and ralstonia eutropha) were substituted for the clostridial enzymes and their ... | 2008 | 19055772 |
| enhanced butanol production by clostridium beijerinckii ba101 grown in semidefined p2 medium containing 6 percent maltodextrin or glucose. | dramatically elevated levels of butanol and acetone resulted in higher butanol and total solvent yields for hyperamylolytic clostridium beijerinckii ba101 relative to the ncimb 8052 parent strain grown in semidefined p2 medium containing either 6% glucose or star-dri 5 maltodextrin. c. beijerinckii ba101 consistently produced on the order of 19 g of butanol per liter in 20-liter batch fermentations. this represents a greater than 100% increase in butanol concentration by the ba101 strain compare ... | 1997 | 16535628 |
| characterisation of a transposon-induced pleiotropic mutant of clostridium acetobutylicum p262. | transposon-induced metronidazole resistance was used as a selection system for the isolation of clostridium acetobutylicum p262 mutants with altered electron transport pathways. the metronidazole resistant transconjugant of interest, mutant 3r, displayed resistance to dna damaging agents, uv and bleomycin, and harboured a single transposon insertion within a structural gene, designated sum(susceptibility to metronidazole). the sum gene encoded a 334 amino-acid protein, with 36% identity and 57-5 ... | 1997 | 16887617 |
| butanol fermentation research: upstream and downstream manipulations. | an overview of advances in acetone-butanol fermentation research is presented with specific reference to the history of acetone-butanol fermentation, genetic manipulation of the butanol-producing clostridium beijerinckii ncimb 8052, as well as upstream and downstream processing. specific reference is made to the development of the hyperamylolytic, hyper-"butanolagenic" c. beijerinckii ba101 strain. amylolytic enzyme production by c. beijerinckii ba101 was 1.8- and 2.5-fold greater than that of t ... | 2004 | 15543610 |
| the circular bacteriocins gassericin a and circularin a. | gassericin a, a bacteriocin produced by lactobacillus gasseri la39, shows antibacterial activity against a number of gram-positive food-borne pathogenic bacteria. circularin a produced by clostridium beijerinckii atcc25752 is active against c. tyrobutyricum, a known cheese-spoilage bacterium. both bacteriocins were purified to homogeneity from culture supernatants by reverse-phase chromatography and the subsequently determined amino acid sequences were used to clone the bacteriocin structural ge ... | 2004 | 15544534 |
| acetone butanol ethanol (abe) production from concentrated substrate: reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping. | acetone butanol ethanol (abe) was produced in an integrated fed-batch fermentation-gas stripping product-recovery system using clostridium beijerinckii ba101, with h(2) and co(2) as the carrier gases. this technique was applied in order to eliminate the substrate and product inhibition that normally restricts abe production and sugar utilization to less than 20 g l(-1) and 60 g l(-1), respectively. in the integrated fed-batch fermentation and product recovery system, solvent productivities were ... | 2004 | 12910325 |
| biochemical and structural properties of chimeras constructed by exchange of cofactor-binding domains in alcohol dehydrogenases from thermophilic and mesophilic microorganisms. | the cofactor-binding domains (residues 153-295) of the alcohol dehydrogenases from the thermophile thermoanaerobacter brockii (tbadh), the mesophilic bacterium clostridium beijerinckii (cbadh), and the protozoan parasite entamoeba histolytica (ehadh1) have been exchanged. three chimeras have been constructed. in the first chimera, the cofactor-binding domain of thermophilic tbadh was replaced with the cofactor-binding domain of its mesophilic counterpart cbadh [chimera chi21((tct))]. this domain ... | 2010 | 20102159 |
| continuous butanol fermentation and feed starch retrogradation: butanol fermentation sustainability using clostridium beijerinckii ba101. | use of starch solution as feed for butanol bioconversion processes employing clostridium beijerinckii ba101 may have added economic advantage over the use of glucose. acetone butanol ethanol (abe) was produced from 30 gl(-1) starch solution using a continuous process. the bioreactor was fed at a dilution rate of 0.02 h(-1) and starch solution/feed volume (3 l) was replaced every 72 h. the continuous reactor fed with cornstarch solution (feed temperature 19 degrees c) produced approximately 6.0 g ... | 2005 | 15607236 |
| functional analysis of the gene cluster involved in production of the bacteriocin circularin a by clostridium beijerinckii atcc 25752. | a region of 12 kb flanking the structural gene of the cyclic antibacterial peptide circularin a of clostridium beijerinckii atcc 25752 was sequenced, and the putative proteins involved in the production and secretion of circularin a were identified. the genes are tightly organized in overlapping open reading frames. heterologous expression of circularin a in enterococcus faecalis was achieved, and five genes were identified as minimally required for bacteriocin production and secretion. two of t ... | 2003 | 14532033 |
| maintenance of deltaph by a butanol-tolerant mutant of clostridium beijerinckii. | the isolation of clostridium beijerinckii mutants that are more tolerant of butanol than the wild-type offered the opportunity to investigate whether the membrane activities which are required for maintaining the transmembrane deltaph (the difference in ph between the cellular interior and exterior) are sensitive targets of butanol toxicity. the deltaph was measured by the accumulation of [14c]benzoate using late-exponential-phase cells which were suspended in citrate/phosphate buffer at ph 5 (t ... | 2005 | 15699209 |
| characterization of the sol operon in butanol-hyperproducing clostridium saccharoperbutylacetonicum strain n1-4 and its degeneration mechanism. | the solventogenic sol operon consisting of bld, ctfa, ctfb, and adc was cloned from clostridium saccharoperbutylacetonicum strain n1-4. these genes share as high as 95-98% similarity with the corresponding sol genes of clostridium beijerinckii ncimb 8052. the n1-4 sol gene cluster was transcribed in a polycistronic manner under the control of two promoters, and its transcription was highly induced during solventogenesis. strain dgn3-4, the degenerated strain derived from n1-4, maintained the sol ... | 2007 | 17213660 |
| improving performance of a gas stripping-based recovery system to remove butanol from clostridium beijerinckii fermentation. | the effect of factors such as gas recycle rate, bubble size, presence of acetone, and ethanol in the solution/broth were investigated in order to remove butanol from model solution or fermentation broth (also called acetone butanol ethanol or abe or solvents). butanol (8 g l(-1), model solution, fig. 2) stripping rate was found to be proportional to the gas recycle rate. in the bubble size range attempted (< 0.5 and 0.5-5.0 mm), the bubble size did not have any effect on butanol removal rate (fi ... | 2005 | 15806382 |
| evidence for the presence of an alternative glucose transport system in clostridium beijerinckii ncimb 8052 and the solvent-hyperproducing mutant ba101. | the effects of substrate analogs and energy inhibitors on glucose uptake and phosphorylation by clostridium beijerinckii provide evidence for the operation of two uptake systems: a previously characterized phosphoenolpyruvate-dependent phosphotransferase system (pts) and a non-pts system probably energized by the transmembrane proton gradient. in both wild-type c. beijerinckii ncimb 8052 and the butanol-hyperproducing mutant ba101, pts activity declined at the end of exponential growth, while gl ... | 2005 | 15933048 |
| acetone, isopropanol, and butanol production by clostridium beijerinckii (syn. clostridium butylicum) and clostridium aurantibutyricum. | thirty-four strains representing 15 species of anaerobic bacteria were screened for acetone, isopropanol, and n-butanol (solvent) production. under our culture conditions, several strains of clostridium beijerinckii and c. aurantibutyricum produced at least 40 mm n-butanol (c. acetobutylicum strains produced up to 41 mm n-butanol under similar conditions). both solvent-producing and non-solvent-producing strains of c. beijerinckii have high dna homology with a reference strain of c. beijerinckii ... | 1983 | 16346237 |
| acidic conditions are not obligatory for onset of butanol formation by clostridium beijerinckii (synonym, c. butylicum). | factors that may initiate the metabolic transition for butanol production were investigated in batch cultures of clostridium beijerinckii (synonym, clostridium butylicum) vpi 13436. cultures maintained at ph 6.8 produced nearly as much butanol as those incubated without ph control, indicating that neither a change in the culture ph nor acid conditions per se are always required to initiate solvent formation. acetate and butyrate levels at the onset of butanol production were dependent on the ph ... | 1983 | 16346358 |
| expression of solvent-forming enzymes and onset of solvent production in batch cultures of clostridium beijerinckii ("clostridium butylicum"). | clostridium beijerinckii ("clostridium butylicum") nrrl b592 and nrrl b593 were grown in batch cultures without ph control. the use of more sensitive and accurate procedures for the determination of solvents in cultures led to the recognition of the onset of solvent production about 2 h earlier than the previously assigned point and at a higher culture ph for both strains. reliable assays for solvent-forming enzyme activities in cell extracts have also been developed. the results showed that act ... | 1988 | 16347575 |
| long-term continuous cultivation of clostridium beijerinckii in a two-stage chemostat with on-line solvent removal. | a two-stage continuous cultivation experiment with clostridium beijerinckii nrrl b592 is described. this strain maintained its ability to produce neutral solvents (acetone, n-butanol, and ethanol) at an overall dilution rate of 0.13 h(sup-1) and achieved an average overall solvent concentration of 9.27 g/liter and an overall solvent productivity of 1.24 g/liter/h for more than 100 overall retention times. the experiment was performed without ph control on a semisynthetic medium containing yeast ... | 1996 | 16535396 |
| changes in numbers and kinds of bacteria during a chickpea submerged fermentation used as a leavening agent for bread production. | the microflora developed during a submerged fermentation of coarsely ground chickpea (cicer arietinum l.) in water (primary starter) and during raising a dough from wheat flour (adapted starter) was studied. in the fermenting liquid, only populations of bacillus and clostridium developed. bacilli increased their loads significantly (p<0.05) during fermentation for 8-12 h and then remained constant. clostridia developed (p<0.05) subsequently to levels of 10(7) cfu/ml at 18 h, when the ph of the f ... | 2007 | 17300848 |
| butanol production from wheat straw hydrolysate using clostridium beijerinckii. | in these studies, butanol (acetone butanol ethanol or abe) was produced from wheat straw hydrolysate (wsh) in batch cultures using clostridium beijerinckii p260. in control fermentation 48.9 g l(-1) glucose (initial sugar 62.0 g l(-1)) was used to produce 20.1 g l(-1) abe with a productivity and yield of 0.28 g l(-1 )h(-1) and 0.41, respectively. in a similar experiment where wsh (60.2 g l(-1) total sugars obtained from hydrolysis of 86 g l(-1) wheat straw) was used, the culture produced 25.0 g ... | 2007 | 17609986 |
| co-regulation of the nitrogen-assimilatory gene cluster in clostridium saccharobutylicum. | nitrogen assimilation is important during solvent production by clostridium saccharobutylicum ncp262, as acetone and butanol yields are significantly affected by the nitrogen source supplied. growth of this bacterium was dependent on the concentration of organic nitrogen supplied and the expression of the assimilatory enzymes, glutamine synthetase (gs) and glutamate synthase (gogat), was shown to be induced in nitrogen-limiting conditions. the regions flanking the gene encoding gs, glna, were is ... | 2007 | 17768251 |
| nutritional requirements of solventogenic clostridia growing under butanol stress and co-utilization of d-glucose/d-xylose. a meta-analysis and a functional validation. | recent advances in systems biology, omics, and computational studies allow us to carry out data mining for improving biofuel production bioprocesses. of particular interest are bioprocesses that center on microbial capabilities to biotransform both the hexose and the pentose fractions present in crop residues. this called for a systematic exploration of media components to obtain higher density cultures and more productive fermentation operations than are currently found. by using a meta-analysi ... | 2011 | 21602379 |
| production of acetone butanol (ab) from liquefied corn starch, a commercial substrate, using clostridium beijerinckii coupled with product recovery by gas stripping. | a potential industrial substrate (liquefied corn starch; lcs) has been employed for successful acetone butanol ethanol (abe) production. fermentation of lcs (60 g l(-1)) in a batch process resulted in the production of 18.4 g l(-1) abe, comparable to glucose: yeast extract based medium (control experiment, 18.6 g l(-1) abe). a batch fermentation of lcs integrated with product recovery resulted in 92% utilization of sugars present in the feed. when abe was recovered by gas stripping (to relieve i ... | 2007 | 17926074 |
| butanol production from crystalline cellulose by co-cultured clostridium thermocellum and clostridium saccharoperbutylacetonicum n1-4. | we investigated butanol production from crystalline cellulose by co-cultured cellulolytic clostridium thermocellum and the butanol-producing strain, c. saccharoperbutylacetonicum (strain n1-4). butanol was produced from avicel cellulose after incubation with c. thermocellum for at least 24 h at 60°c before adding strain n1-4. butanol produced by strain n1-4 on 4% avicel cellulose peaked (7.9 g/l) after 9 days of incubation at 30°c and acetone was undetectable in this co-culture system. less buta ... | 2011 | 21764954 |
| engineered synthetic pathway for isopropanol production in escherichia coli. | a synthetic pathway was engineered in escherichia coli to produce isopropanol by expressing various combinations of genes from clostridium acetobutylicum atcc 824, e. coli k-12 mg1655, clostridium beijerinckii nrrl b593, and thermoanaerobacter brockii htd4. the strain with the combination of c. acetobutylicum thl (acetyl-coenzyme a [coa] acetyltransferase), e. coli atoad (acetoacetyl-coa transferase), c. acetobutylicum adc (acetoacetate decarboxylase), and c. beijerinckii adh (secondary alcohol ... | 2007 | 17933911 |
| fermentation of dried distillers' grains and solubles (ddgs) hydrolysates to solvents and value-added products by solventogenic clostridia. | pretreatment and hydrolysis of lignocellulosic biomass using either dilute acid, liquid hot water (lhw), or ammonium fiber expansion (afex) results in a complex mixture of sugars such as hexoses (glucose, galactose, mannose), and pentoses (xylose, arabinose). a detailed description of the utilization of representative mixed sugar streams (pentoses and hexoses) and their sugar preferences by the solventogenic clostridia (clostridium beijerinckii ba101, c. acetobutylicum 260, c. acetobutylicum 824 ... | 2008 | 17967532 |
| influence of reduced electron shuttling compounds on biological h2 production in the fermentative pure culture clostridium beijerinckii. | several reports suggest that extracellular electron shuttles influence fermentative metabolism in a beneficial manner for bioremediation and biotechnology strategies. the focus of this research was to characterize the effects of reduced electron shuttling molecules on fermentative h(2) production. reduced electron shuttles may provide reducing equivalents to generate h(2), which influences alternate cellular processes. electron shuttling compounds cycle between reduced-oxidized states and influe ... | 2008 | 18167025 |
| experience with solvent production by clostridium beijerinckii in continuous culture. | | 1983 | 18548731 |
| chitinolytic activities of clostridium sp. jm2 isolated from stool of human administered per orally by chitosan. | the novel chitinolytic bacterium clostridium beijerinckii strain jm2 was isolated from the stool of healthy volunteers supplied daily per orally with 3 g of chitosan. the bacterium grown on colloidal chitin produced a complete array of chitinolytic enzymes. significant activities of endochitinase, exochitinase and chitosanase were excreted into the medium (301, 282 and 268 nkat/microg protein, respectively). the high cellular activity of n-acetyl-beta-glucosaminidase (nagase) and chitosanase wer ... | 2008 | 18661303 |
| detoxification of model phenolic compounds in lignocellulosic hydrolysates with peroxidase for butanol production from clostridium beijerinckii. | in the present study, we investigated the peroxidase-catalyzed detoxification of model phenolic compounds and evaluated the inhibitory effects of the detoxified solution on butanol production by clostridium beijerinckii national collection of industrial and marine bacteria ltd. 8052. the six phenolic compounds, p-coumaric acid, ferulic acid, 4-hydroxybenzoic acid, vanillic acid, syringaldehyde, and vanillin, were selected as model fermentation inhibitors generated during pretreatment and hydroly ... | 2009 | 19300996 |
| fermentation of rice bran and defatted rice bran for butanol 5 production using clostridium beijerinckii ncimb 8052. | we examined butanol fermentation by clostridium beijerinckii ncimb 8052 using various hydrolyzates obtained from rice bran, which is one of the most abundant agricultural by-products in korea and japan. in order to increase the amount of fermentable sugars in the hydrolyzates of rice bran, various hydrolysis procedures were applied. eight different hydrolyzates were prepared using rice bran (rb) and defatted rice bran (drb) with enzyme or acid treatment or both. each hydrolyzate was evaluated in ... | 2009 | 19494696 |
| isolation of a new butanol-producing clostridium strain: high level of hemicellulosic activity and structure of solventogenesis genes of a new clostridium saccharobutylicum isolate. | new isolates of solventogenic bacteria exhibited high hemicellulolytic activity. they produced butanol and acetone with high selectivity for butanol (about 80% of butanol from the total solvent yield). their 16s rdna sequence was 99% identical to that of clostridium saccharobutylicum. the genes responsible for the last steps of solventogenesis and encoding crotonase, butyryl-coa dehydrogenase, electron-transport protein subunits a and b, 3-hydroxybutyryl-coa dehydrogenase, alcohol dehydrogenase, ... | 2009 | 19674858 |
| the clostron: mutagenesis in clostridium refined and streamlined. | the recent development of the clostron group ii intron directed mutagenesis tool for clostridium has advanced genetics in this genus, and here we present several significant improvements. we have shown how marker re-cycling can be used to construct strains with multiple mutations, demonstrated using flp/frt in clostridium acetobutylicum; tested the capacity of the system for the delivery of transgenes to the chromosome of clostridium sporogenes, which proved feasible for 1.0kbp transgenes in add ... | 2010 | 19891996 |
| comparison of different mixed cultures for bio-hydrogen production from ground wheat starch by combined dark and light fermentation. | composition of the mixed culture was varied in combined dark-light fermentation of wheat powder starch in order to improve hydrogen gas formation rate and yield. heat-treated anaerobic sludge and pure culture of clostridium beijerinckii (dsmz 791t) were combined with two different light fermentation bacteria of rhodobacter sphaeroides (rs-nrrl and rs-rv) in order to select a more suitable mixture resulting in high hydrogen yield and formation rate. a combination of the anaerobic sludge and rs-nr ... | 2010 | 20033469 |
| interactions between bacillus cereus cgmcc 1.895 and clostridium beijerinckii ncimb 8052 in coculture for butanol production under nonanaerobic conditions. | low oxygen tolerance and substrate restriction continues to hamper the process of biobutanol industrialization. in this work, butanol fermentation with cocultures of bacillus cereus china general microbiological culture collection center (cgmcc) 1.895 and clostridium beijerinckii ncimb 8052 under nonanaerobic conditions was investigated, and the interactions between these two strains were examined. the addition of b. cereus cgmcc 1.895 resulted in higher oxygen tolerance and a wider range of sub ... | 2016 | 27306691 |
| functional expression of the thiolase gene thl from clostridium beijerinckii p260 in lactococcus lactis and lactobacillus buchneri. | the first step of the butanol pathway involves an acetyl-coa acetyltransferase (acoaat), which controls the key branching point from acetyl-coa to butanol. acoaat, also known as thiolase (ec 2.3.1.9), is encoded by the thl gene and catalyzes ligation of two acetyl-coa into acetoacetyl-coa. bioinformatics analyses suggest there are no thl in the genomes of lactic acid bacteria (lab), in this study we aimed to introduce the thl gene into selected lab strains and analyze the fermentation products. ... | 2010 | 20371307 |
| butanol production by clostridium beijerinckii atcc 55025 from wheat bran. | wheat bran, a by-product of the wheat milling industry, consists mainly of hemicellulose, starch and protein. in this study, the hydrolysate of wheat bran pretreated with dilute sulfuric acid was used as a substrate to produce abe (acetone, butanol and ethanol) using clostridium beijerinckii atcc 55025. the wheat bran hydrolysate contained 53.1 g/l total reducing sugars, including 21.3 g/l of glucose, 17.4 g/l of xylose and 10.6 g/l of arabinose. c. beijerinckii atcc 55025 can utilize hexose and ... | 2010 | 20393827 |
| lipid diversity among botulinum neurotoxin-producing clostridia. | clostridium botulinum has been classified into four groupings (groups i to iv) based on physiological characteristics and 16s rrna sequencing. we have examined the lipid compositions of 11 representative strains of c. botulinum and a strain of clostridium sporogenes by 2d-tlc and by ms. all strains contained phosphatidylglycerol (pg), cardiolipin (cl) and phosphatidylethanolamine (pe) in both the all-acyl and the alk-1'-enyl (plasmalogen) forms. five strains in proteolytic group i, which are rel ... | 2012 | 22837302 |
| the t box regulatory element controlling expression of the class i lysyl-trna synthetase of bacillus cereus strain 14579 is functional and can be partially induced by reduced charging of asparaginyl-trnaasn. | lysyl-trna synthetase (lysrs) is unique within the aminoacyl-trna synthetase family in that both class i (lysrs1) and class ii (lysrs2) enzymes exist. lysrs1 enzymes are found in archaebacteria and some eubacteria while all other organisms have lysrs2 enzymes. all sequenced strains of bacillus cereus (except ah820) and bacillus thuringiensis however encode both a class i and a class ii lysrs. the lysk gene (encoding lysrs1) of b. cereus strain 14579 has an associated t box element, the first rep ... | 2010 | 20649968 |
| acetone production in solventogenic clostridium species: new insights from non-enzymatic decarboxylation of acetoacetate. | development of a butanologenic strain with high selectivity for butanol production is often proposed as a possible route for improving the economics of biobutanol production by solventogenic clostridium species. the acetoacetate decarboxylase (aadc) gene encoding acetoacetate decarboxylase (aadc), which catalyzes the decarboxylation of acetoacetate into acetone and co(2), was successfully disrupted by homologous recombination in solventogenic clostridium beijerinckii ncimb 8052 to generate an aa ... | 2011 | 21533580 |
| continuous production of isopropanol and butanol using clostridium beijerinckii dsm 6423. | clostridium beijerinckii dsm 6423 was studied using different continuous production methods to give maximum and stable production of isopropanol and n-butanol. in a single-stage continuous culture, when wood pulp was added as a cell holding material, we could increase the solvent productivity from 0.47 to 5.52 g l(-1) h(-1) with the yield of 54% from glucose. the overall solvent concentration of 7.51 g l(-1) (39.4% isopropanol and 60.6% n-butanol) with the maximum solvent productivity of 0.84 g ... | 2011 | 21573939 |
| clostridium beijerinckii mutant with high inhibitor tolerance obtained by low-energy ion implantation. | clostridium beijerinckii mutant strain ib4, which has a high level of inhibitor tolerance, was screened by low-energy ion implantation and used for butanol fermentation from a non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (sahhc). evaluation of toxicity showed c. beijerinckii ib4 had a higher level of tolerance than parent strain c. beijerinckii ncimb 8052 for five out of six phenolic compounds tested (the exception was vanillin). using glucose as carb ... | 2011 | 21789489 |
| clostridium beijerinckii mutant obtained by atmospheric pressure glow discharge producing high proportions of butanol and solvent yields. | with 30-ág glucose/l as carbon source, clostridium beijerinckii art124, a mutant created by atmospheric pressure glow discharge, produced 13.7-ág total solvent/l (containing 3.1-ág acetone/l, 10.4-ág butanol/l and 0.2-ág ethanol/l) in 72-áh. the mutant could also use sucrose or xylose or a mixture of glucose/xylose/arabinose with nearly equal yields. | 2011 | 21833546 |
| Metabolic Engineering of Clostridium acetobutylicum ATCC 824 for Isopropanol-Butanol-Ethanol Fermentation. | Clostridium acetobutylicum naturally produces acetone as well as butanol and ethanol. Since acetone cannot be used as a biofuel, its production needs to be minimized or suppressed by cell or bioreactor engineering. Thus, there have been attempts to disrupt or inactivate the acetone formation pathway. Here we present another approach, namely converting acetone to isopropanol by metabolic engineering. Since isopropanol can be used as a fuel additive, the mixture of isopropanol, butanol, and ethano ... | 2011 | 22210214 |
| outgrowth inhibition of clostridium beijerinckii spores by a bacteriocin-producing lactic culture in ovine milk cheese. | in the manufacture of model cheeses, ovine milk was deliberately contaminated with spores of clostridium beijerinckii inia 63, a wild isolate from manchego cheese with late blowing defect, and inoculated with nisin- and lacticin 481-producing lactococcus lactis subsp. lactis inia 415 as starter, to test its potential to prevent the late blowing defect, or with l. lactis subsp. lactis inia 415-2, a spontaneous mutant not producing bacteriocins. cheeses made individually with the lactococcal strai ... | 2011 | 21849216 |
| clostridium chromiireducens sp. nov., a novel strain isolated from a cr(vi)-contaminated soil. | strain gcaf-1 is a cr(vi)-resistant, gram positive, spore-forming obligate anaerobe that was 2 isolated from a chromium-contaminated soil by its ability to reduce cr(vi) in low 3 concentrations, fe(iii), and the humic-acid analog aqds. a mixed acid fermentation resulting 4 in accumulation of acetate, butyrate and lactate is observed during growth of gcaf-1 on 5 glucose. morphological studies on this strain indicate the presence of peritrichous flagella, pili 6 and an s-layer. major cellular fatt ... | 2010 | 21148674 |
| large number of phosphotransferase genes in the clostridium beijerinckii ncimb 8052 genome and the study on their evolution. | clostridium beijerinckii is a valuable bacteria species which has the ability of abe (acetone, butanol and ethanol) production. it has been shown that phosphotransferase (pts) is an important and common system for both carbohydrate uptake and phosphorylation in bacteria, but detailed study of the system, especially its fructose/mannose/sorbose family is scant. | 2010 | 21172059 |
| d-2,3-butanediol production due to heterologous expression of an acetoin reductase in clostridium acetobutylicum. | acetoin reductase (acr) catalyzes the conversion of acetoin to 2,3-butanediol. under certain conditions, clostridium acetobutylicum atcc 824 (and strains derived from it) generates both d- and l-stereoisomers of acetoin, but because of the absence of an acr enzyme, it does not produce 2,3-butanediol. a gene encoding acr from clostridium beijerinckii ncimb 8052 was functionally expressed in c. acetobutylicum under the control of two strong promoters, the constitutive thl promoter and the late exp ... | 2011 | 21335380 |
| optimized transformation of newly constructed escherichia coli-clostridia shuttle vectors into clostridium beijerinckii. | three escherichia coli-clostridia shuttle vectors, pkba411-mcs, pkbe411-mcs, and pkbm411-mcs, which contain p15a, cole1, and pmb1 origins for replication in e. coli, respectively, along with the pamb origin for replication in c. beijerinckii, were constructed and examined for their transformation efficiencies into clostridium beijerinckii ncimb8052. the transformation condition of pkbm411-mcs, which was optimized by varying resistance, buffer composition, and dna concentration, was further emplo ... | 2015 | 26152821 |
| continuous production of 1,3-propanediol using raw glycerol with immobilized clostridium beijerinckii nrrl b-593 in comparison to suspended culture. | the continuous production of 1,3-propanediol (1,3-pdo) was investigated with clostridium beijerinckii nrrl b-593 using raw glycerol without purification obtained from a biodiesel production process. ceramic rings and pumice stones were used for cell immobilization in a packed-bed bioreactor. for comparison purpose, a control bioreactor with suspended culture was also run. the effect of hydraulic retention time (hrt) on the production of 1,3-pdo in both immobilized and suspended bioreactors were ... | 2011 | 21336641 |
| bioproduction of butanol in bioreactors: new insights from simultaneous in situ butanol recovery to eliminate product toxicity. | simultaneous acetone butanol ethanol (abe) fermentation by clostridium beijerinckii p260 and in situ product recovery was investigated using a vacuum process operated in two modes: continuous and intermittent. integrated batch fermentations and abe recovery were conducted at 37 °c using a 14-l bioreactor (7.0 l fermentation volume) containing initial substrate (glucose) concentration of 60 g/l. the bioreactor was connected in series with a condensation system and vacuum pump. vacuum was applied ... | 2011 | 21370232 |
| hydrogen production by the newly isolated clostridium beijerinckii rzf-1108. | a fermentative hydrogen-producing strain, rzf-1108, was isolated from a biohydrogen reactor, and identified as clostridium beijerinckii on the basis of the 16s rrna gene analysis and physiobiochemical characteristics. the effects of culture conditions on hydrogen production by c. beijerinckii rzf-1108 were investigated in batch cultures. the hydrogen production and growth of strain rzf-1108 were highly dependent on temperature, initial ph and substrate concentration. yeast extract was a favorabl ... | 2011 | 21421301 |
| improved efficiency of separate hexose and pentose fermentation from steam-exploded corn stalk for butanol production using clostridium beijerinckii. | water extract of steam-exploded corn stalk (secs) was detoxified and used as feed for acetone-butanol-ethanol (abe) fermentation using clostridium beijerinckii. utilization of water extract improved the total abe yield (g abe/g dry secs). separated fermentation showed higher fermentability (0.078 g abe/g dry secs) over typical fermentation (0.058 g abe/g dry secs). furthermore, the final abe yields (g abe/g utilized sugar) from water extract neutralized by ca(oh)(2), naoh, and na(2)so(3) were 0. ... | 2011 | 21424838 |
| butanol production from the effluent of hydrogen fermentation. | the purpose of the study was to recover butanol from the effluent of the hydrogen-producing bioreactor containing acetate, butyrate, and carbohydrate. the butanol production by clostridium beijerinckii nrrl b592 was evaluated under both unsterilized and sterilized conditions for examining the potential of butanol production for the practical application. sucrose of 10 g/l and butyrate of 2 g/l coupled with acetate buffer were used to mimic the effluent. sucrose was completely consumed in the bot ... | 2011 | 21436562 |
| impact of organic loading rate on biohydrogen production in an up-flow anaerobic packed bed reactor (uanpbr). | this study assesses the impact of organic loading rate on biohydrogen production from glucose in an up-flow anaerobic packed bed reactor (uanpbr). two mesophilic uapbrs (uanpbr1 and 2) were tested at organic loading rates (olrs) ranging from 6.5 to 51.4 g cod l(-1)d(-1). to overcome biomass washout, design modifications were made in the uanpbr2 to include a settling zone to capture the detached biomass. the design modifications in uanpbr2 increased the average hydrogen yield from 0.98 to 2.0 mol ... | 2014 | 24865326 |
| acetone-butanol-ethanol production from substandard and surplus dates by egyptian native clostridium strains. | one hundred and seven mesophilic isolates of clostridium were isolated from agricultural soils cultivated with different plants in assuit governorate, egypt. eighty isolates (out of 107) showed the ability to produce abe (acetone, butanol and ethanol) on t6 medium ranging from 0.036 to 31.89 g/l. the highest numbers of abe producing isolates were obtained from soil samples of potato contributing 27 isolates, followed by 18 isolates from wheat and 10 isolates from onion. on the other hand, there ... | 2015 | 25557787 |
| development of a triplex real-time pcr assay for the simultaneous detection of clostridium beijerinckii, clostridium sporogenes and clostridium tyrobutyricum in milk. | clostridium beijerinckii, clostridium sporogenes and clostridium tyrobutyricum are considered the leading bacteria implicated in late blowing defects affecting semi-hard and hard cheese production. the aim of this study was to develop a multiplex real-time pcr (qpcr) analysis for a rapid and simultaneous detection of c. beijerinckii, c. sporogenes and c. tyrobutyricum, using specific primers respectively targeting the nifh, geraa and enr genes. the limits of detection in raw milk were 300 cfu/50 ... | 2015 | 25870135 |
| single-nucleotide resolution analysis of the transcriptome structure of clostridium beijerinckii ncimb 8052 using rna-seq. | clostridium beijerinckii is an important solvent producing microorganism. the genome of c. beijerinckii ncimb 8052 has recently been sequenced. although transcriptome structure is important in order to reveal the functional and regulatory architecture of the genome, the physical structure of transcriptome for this strain, such as the operon linkages and transcript boundaries are not well understood. | 2011 | 21962126 |
| anthrahydroquinone-2,6,-disulfonate (ah2qds) increases hydrogen molar yield and xylose utilization in growing cultures of clostridium beijerinckii. | h(2) production and xylose utilization were investigated using the fermentative culture clostridium beijerinckii ncimb 8052. adding anthrahydroquinone-2,6-disulfonate (ah(2)qds) increased the extent of xylose utilization by 56% and hydrogen molar yield by 24-37%. enhanced hydrogen molar yield correlated with increased xylose utilization and increases in the acetate/butyrate product ratio. an electron balance indicated that ah(2)qds shifted the electrons from the butyric acid pathway (nadh-depend ... | 2011 | 21947605 |
| metabolic network reconstruction and genome-scale model of butanol-producing strain clostridium beijerinckii ncimb 8052. | solventogenic clostridia offer a sustainable alternative to petroleum-based production of butanol--an important chemical feedstock and potential fuel additive or replacement. c. beijerinckii is an attractive microorganism for strain design to improve butanol production because it (i) naturally produces the highest recorded butanol concentrations as a byproduct of fermentation; and (ii) can co-ferment pentose and hexose sugars (the primary products from lignocellulosic hydrolysis). interrogating ... | 2011 | 21846360 |
| Optimization of butanol production from tropical maize stalk juice by fermentation with Clostridium beijerinckii NCIMB 8052. | Mixed sugars from tropical maize stalk juice were used to carry out butanol fermentation with Clostridium beijerinckii NCIMB 8052. Batch experiments employing central composite design (CCD) and response surface methodology (RSM) optimization were performed to evaluate effects of three factors, i.e. pH, initial total sugar concentration, and agitation rate on butanol production. Optimum conditions of pH 6.7, sugar concentration 42.2g/L and agitation rate 48 rpm were predicted, under which a maxim ... | 2011 | 21893411 |
| effects of nisin and reutericyclin on resistance of endospores of clostridium spp. to heat and high pressure. | the effects of high pressure, temperature, and antimicrobial compounds on endospores of clostridium spp. were examined. minimal inhibitory concentrations (mic) of nisin and reutericyclin were determined for vegetative cells and endospores of clostridium sporogenes atcc 7955, clostridium beijerinckii atcc 8260, and clostridium difficile 3195. endospores of c. sporogenes atcc 7955 and c. beijerinckii atcc 8260 were exposed to 90 °c and 90 °c/600 mpa in the presence of 16 mg l(-1) nisin or 6.4 mg l ... | 2012 | 23498177 |
| use of proteomic analysis to elucidate the role of calcium in acetone-butanol-ethanol fermentation by clostridium beijerinckii ncimb 8052. | calcium carbonate increases growth, substrate utilization, and acetone-butanol-ethanol (abe) fermentation by clostridium beijerinckii ncimb 8052. toward an understanding of the basis for these pleiotropic effects, we profiled changes in the c. beijerinckii ncimb 8052 proteome that occur in response to the addition of caco(3). we observed increases in the levels of different heat shock proteins (grpe and dnak), sugar transporters, and proteins involved in dna synthesis, repair, recombination, and ... | 2013 | 23104411 |
| transcriptional analysis of clostridium beijerinckii ncimb 8052 to elucidate role of furfural stress during acetone butanol ethanol fermentation. | furfural is the prevalent microbial inhibitor generated during pretreatment and hydrolysis of lignocellulose biomass to monomeric sugars, but the response of acetone butanol ethanol (abe) producing clostridium beijerinckii ncimb 8052 to this compound at the molecular level is unknown. to discern the effect of furfural on c. beijerinckii and to gain insight into molecular mechanisms of action and detoxification, physiological changes of furfural-stressed cultures during acetone butanol ethanol (a ... | 2013 | 23642190 |
| the antimicrobial action of chitosan, low molar mass chitosan, and chitooligosaccharides on human colonic bacteria. | antibacterial effect of chitooligosaccharides (cos) and low molar mass chitosans (lmwc) is considered as one of the most important characteristics of chitosan (cs) hydrolysates. here, we show the in vitro effect of different cos, lmwc, and cs on representative anaerobic bacteria isolated from human colon as a possibility of targeting modification of colonic microflora composition by supplementation of dietary cs products by humans. specific growth rate of seven selected nonpathogenic anaerobic b ... | 2012 | 22528310 |
| a roadmap for gene system development in clostridium. | clostridium species are both heroes and villains. some cause serious human and animal diseases, those present in the gut microbiota generally contribute to health and wellbeing, while others represent useful industrial chassis for the production of chemicals and fuels. to understand, counter or exploit, there is a fundamental requirement for effective systems that may be used for directed or random genome modifications. we have formulated a simple roadmap whereby the necessary gene systems maybe ... | 2016 | 27234263 |
| genomic approach to studying nutritional requirements of clostridium tyrobutyricum and other clostridia causing late blowing defects. | clostridium tyrobutyricum is the main microorganism responsible for the late blowing defect in hard and semi-hard cheeses, causing considerable economic losses to the cheese industry. deeper knowledge of the metabolic requirements of this microorganism can lead to the development of more effective control approaches. in this work, the amino acids and b vitamins essential for sustaining the growth of c. tyrobutyricum were investigated using a genomic approach. as the first step, the genomes of fo ... | 2016 | 27375262 |
| seasonal occurrence and molecular diversity of clostridia species spores along cheesemaking streams of 5 commercial dairy plants. | five commercial dairy plants were monitored over a 17-mo period to determine the seasonal occurrence of clostridium spores in streams from the cheesemaking process. every 2 mo, samples of raw milk (rm), separated cream (sc), pasteurized and standardized vat milk (psvm), psvm + lysozyme (psvm+l), and manufactured cheese aged for 60 to 90 d were processed for analysis. molecular diversity of the main species identified was determined using repetitive element palindromic pcr. the mean anaerobic spo ... | 2016 | 26923043 |
| gene transcription repression in clostridium beijerinckii using crispr-dcas9. | crispr-cas9 has been explored as a powerful tool for genome engineering for many organisms. meanwhile, dcas9 which lacks endonuclease activity but can still bind to target loci has been engineered for efficient gene transcription repression. clostridium beijerinckii, an industrially significant species capable of biosolvent production, is generally difficult to metabolically engineer. recently, we reported our work in developing customized crispr-cas9 system for genome engineering in c. beijerin ... | 2016 | 27240718 |
| crispr-based genome editing and expression control systems in clostridium acetobutylicum and clostridium beijerinckii. | solventogenic clostridia are important industrial microorganisms that produce various chemicals and fuels. effective genetic tools would facilitate physiological studies aimed both at improving our understanding of metabolism and optimizing solvent productivity through metabolic engineering. here we have developed an all-in-one, crispr-based genome editing plasmid, pnickclos, that can be used to achieve successive rounds of gene editing in clostridium acetobutylicum atcc 824 and clostridium beij ... | 2016 | 27213844 |
| bacterial genome editing with crispr-cas9: deletion, integration, single nucleotide modification, and desirable "clean" mutant selection in clostridium beijerinckii as an example. | crispr-cas9 has been demonstrated as a transformative genome engineering tool for many eukaryotic organisms; however, its utilization in bacteria remains limited and ineffective. here we explored streptococcus pyogenes crispr-cas9 for genome editing in clostridium beijerinckii (industrially significant but notorious for being difficult to metabolically engineer) as a representative attempt to explore crispr-cas9 for genome editing in microorganisms that previously lacked sufficient genetic tools ... | 2016 | 27115041 |
| use of the fluorescent probe laurdan to label and measure inner membrane fluidity of endospores of clostridium spp. | a method for measuring the fluidity of inner membranes of populations of endospores of clostridium spp. with a fluorescent dye was developed. cells of clostridium beijerinckii atcc 8260 and clostridium sporogenes atcc 7955 were allowed to sporulate in the presence of 6-dodecanoyl-2-dimethylaminonaphthalene (laurdan) on a soil-based media. labeling of endospores with laurdan did not affect endospore viability. removal of the outer membranes of endospores was done using a chemical treatment and co ... | 2012 | 22884687 |
| use of cupriavidus basilensis-aided bioabatement to enhance fermentation of acid-pretreated biomass hydrolysates by clostridium beijerinckii. | lignocellulose-derived microbial inhibitors (ldmics) prevent efficient fermentation of miscanthus giganteus (mg) hydrolysates to fuels and chemicals. to address this problem, we explored detoxification of pretreated mg biomass by cupriavidus basilensis atcc(®)baa-699 prior to enzymatic saccharification. we document three key findings from our test of this strategy to alleviate ldmic-mediated toxicity on clostridium beijerinckii ncimb 8052 during fermentation of mg hydrolysates. first, we demonst ... | 2016 | 27400988 |
| production of 1,3-propanediol by clostridium beijerinckii dsm 791 from crude glycerol and corn steep liquor: process optimization and metabolic engineering. | 1,3-propanediol (1,3-pdo) production from crude glycerol, a byproduct from biodiesel manufacturing, by clostridium beijerinckii dsm 791 was studied with corn steep liquor as an inexpensive nitrogen source replacing yeast extract in the fermentation medium. a stable, long-term 1,3-pdo production from glycerol was demonstrated with cells immobilized in a fibrous bed bioreactor operated in a repeated batch mode, which partially circumvented the 1,3-pdo inhibition problem. the strain was then engine ... | 2016 | 27085150 |
| efficient acetone-butanol-ethanol production by clostridium beijerinckii from sugar beet pulp. | sugar beet pulp (sbp) has been investigated as a promising feedstock for abe fermentation by clostridium beijerinckii. although lignin content in sbp is low, a pretreatment is needed to enhance enzymatic hydrolysis and fermentation yields. autohydrolysis at ph 4 has been selected as the best pretreatment for sbp in terms of sugars release and acetone and butanol production. the best overall sugars release yields from raw sbp ranged from 66.2% to 70.6% for this pretreatment. the highest abe yield ... | 2015 | 25965949 |
| isolation, characterization and evaluation of hyper 2-propanol producing bacteria from singapore environment. | three hyper 2-propanol producing strains were isolated from singapore environment using an enrichment step and a high through-put screening step. the analysis of the amplified 16s rdna revealed that the isolates belonged to clostridium species and they were named as clostridium sp. bt10-1, clostridium sp. m10-1 and clostridium sp. pu31-4. at 1 l scale, the 2-propanol titer of these positive strains was 1.6-2.1 times of that of clostridium beijerinckii nrrl b593, which is so far the most efficien ... | 2013 | 23361972 |
| biosynthesis of the fatty acid isopropyl esters by engineered escherichia coli. | the fatty acid methyl esters and fatty acid ethyl esters are known as biodiesels which are considered to be renewable, nontoxic and biodegradable biofuels. however, the conventional biodiesels show a high crystallization temperature which is one of the most critical obstacles against the widespread biodiesel usage. the high crystallization temperature of biodiesel can be reduced by replacing the methyl or ethyl ester with an isopropyl moiety. here we report on a strategy to establish biosynthesi ... | 2017 | 28465060 |
| identification of a glucose-mannose phosphotransferase system in clostridium beijerinckii. | effective uptake of fermentable substrates is a fundamentally important aspect of any fermentation process. the solventogenic bacterium clostridium beijerinckii is noted for its ability to ferment a wide range of carbohydrates, yet few of its sugar transport systems have been characterized. in common with other anaerobes, c. beijerinckii shows a marked dependence on the pep-dependent phosphotransferase system (pts) for sugar accumulation. in this study, the gene cbe0751 encoding the sugar-specif ... | 2016 | 26940293 |
| construction of heterologous gene expression cassettes for the development of recombinant clostridium beijerinckii. | gene-expression cassettes for the construction of recombinant clostridium beijerinckii were developed as potential tools for metabolic engineering of c. beijerinckii. gene expression cassettes containing cole1 origin and pamb origin along with the erythromycin resistance gene were constructed, in which promoters from escherichia coli, lactococcus lactis, ralstonia eutropha, c. acetobutylicum, and c. beijerinckii are examined as potential promoters in c. beijerinckii. zymogram analysis of the cel ... | 2016 | 26780375 |
| identification, purification and characterization of furfural transforming enzymes from clostridium beijerinckii ncimb 8052. | generation of microbial inhibitory compounds such as furfural and 5-hydroxymethylfurfural (hmf) is a formidable roadblock to fermentation of lignocellulose-derived sugars to butanol. bioabatement offers a cost effective strategy to circumvent this challenge. although clostridium beijerinckii ncimb 8052 can transform 2-3 g/l of furfural and hmf to their less toxic alcohols, higher concentrations present in biomass hydrolysates are intractable to microbial transformation. to delineate the mechanis ... | 2015 | 25796546 |
| molecular characterization of an nadph-dependent acetoin reductase/2,3-butanediol dehydrogenase from clostridium beijerinckii ncimb 8052. | acetoin reductase is an important enzyme for the fermentative production of 2,3-butanediol, a chemical compound with a very broad industrial use. here, we report on the discovery and characterization of an acetoin reductase from clostridium beijerinckii ncimb 8052. an in silico screen of the c. beijerinckii genome revealed eight potential acetoin reductases. one of them (cbei_1464) showed substantial acetoin reductase activity after expression in escherichia coli. the purified enzyme (c. beijeri ... | 2014 | 24441158 |
| engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light. | production of alternate fuels or chemicals directly from solar energy and carbon dioxide using engineered cyanobacteria is an attractive method to reduce petroleum dependency and minimize carbon emissions. here, we constructed a synthetic pathway composed of acetyl-coa acetyl transferase (encoded by thl), acetoacetyl-coa transferase (encoded by atoad), acetoacetate decarboxylase (encoded by adc) and secondary alcohol dehydrogenase (encoded by adh) in synechococcus elongatus strain pcc 7942 to pr ... | 2013 | 24076145 |
| dual substrate specificity of an n-acetylglucosamine phosphotransferase system in clostridium beijerinckii. | the solventogenic clostridia have a considerable capacity to ferment carbohydrate substrates with the production of acetone and butanol, making them attractive organisms for the conversion of waste materials to valuable products. in common with other anaerobes, the clostridia show a marked dependence on the phosphoenolpyruvate (pep)-dependent phosphotransferase system (pts) to accumulate sugars and sugar derivatives. in this study, we demonstrate that extracts of clostridium beijerinckii grown o ... | 2013 | 23995920 |
| metabolic engineering of candida utilis for isopropanol production. | a genetically-engineered strain of the yeast candida utilis harboring genes encoding (1) an acetoacetyl-coa transferase from clostridium acetobutylicum atcc 824, (2) an acetoacetate decarboxylase, and (3) a primary-secondary alcohol dehydrogenase derived from clostridium beijerinckii nrrl b593 produced up to 0.21 g/l of isopropanol. because the engineered strain accumulated acetate, isopropanol titer was improved to 1.2 g/l under neutralized fermentation conditions. optimization of isopropanol p ... | 2013 | 23674152 |
| evaluation of industrial dairy waste (milk dust powder) for acetone-butanol-ethanol production by solventogenic clostridium species. | readily available inexpensive substrate with high product yield is the key to restoring acetone-butanol-ethanol (abe) fermentation to economic competitiveness. lactose-replete cheese whey tends to favor the production of butanol over acetone. in the current study, we investigated the fermentability of milk dust powder with high lactose content, for abe production by clostridium acetobutylicum and clostridium beijerinckii. both microorganisms produced 7.3 and 5.8 g/l of butanol respectively, with ... | 2014 | 25126487 |
| felled oil palm trunk as a renewable source for biobutanol production by clostridium spp. | this study aimed to convert felled oil palm trunk to biobutanol by clostridium spp. for efficient utilization of oil palm trunk, it was separated into sap and trunk fiber. the sap was used directly while the trunk fiber was hydrolyzed to fermentable sugars before use. among five clostridia strains screened, clostridium acetobutylicum dsm 1731 was the most suitable strain for butanol production from the sap without any supplementation of nutrients. it produced the highest amount of butanol (14.4 ... | 2013 | 23933028 |
| production of acetone, butanol, and ethanol from biomass of the green seaweed ulva lactuca. | green seaweed ulva lactuca harvested from the north sea near zeeland (the netherlands) was characterized as feedstock for acetone, ethanol and ethanol fermentation. solubilization of over 90% of sugars was achieved by hot-water treatment followed by hydrolysis using commercial cellulases. a hydrolysate was used for the production of acetone, butanol and ethanol (abe) by clostridium acetobutylicum and clostridium beijerinckii. hydrolysate-based media were fermentable without nutrient supplementat ... | 2013 | 23201525 |
| simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by clostridium acetobutylicum atcc 824 engineered strains. | isopropanol represents a widely-used commercial alcohol which is currently produced from petroleum. in nature, isopropanol is excreted by some strains of clostridium beijerinckii, simultaneously with butanol and ethanol during the isopropanol butanol ethanol (ibe) fermentation. in order to increase isopropanol production, the gene encoding the secondary-alcohol dehydrogenase enzyme from c. beijerinckii nrrl b593 (adh) which catalyzes the reduction of acetone to isopropanol, was cloned into the a ... | 2012 | 22909015 |
| introducing a single secondary alcohol dehydrogenase into butanol-tolerant clostridium acetobutylicum rh8 switches abe fermentation to high level ibe fermentation. | | 2012 | 22742819 |
| [periodontal microbiota and microorganisms isolated from heart valves in patients undergoing valve replacement surgery in a clinic in cali, colombia]. | periodontitis is an infectious disease that affects the support tissue of the teeth and it is associated with different systemic diseases, including cardiovascular disease. microbiological studies facilitate the detection of microorganisms from subgingival and cardiovascular samples. | 2017 | 29373772 |
| renewable synthesis of n-butyraldehyde from glucose by engineered escherichia coli. | n-butyraldehyde is a high-production volume chemical produced exclusively from hydroformylation of propylene. it is a versatile chemical used in the synthesis of diverse c4-c8 alcohols, carboxylic acids, esters, and amines. its high demand and broad applications make it an ideal chemical to be produced from biomass. | 2017 | 29213330 |
| development of an oxygen-independent flavin mononucleotide-based fluorescent reporter system in clostridium beijerinckii and its potential applications. | clostridium beijerinckii is a predominant solventogenic clostridia with great attraction for renewable liquid biofuel and biochemical production. metabolic engineering and synthetic biology can be employed to engineer the strain toward desirable phenotypes. however, current limited information such as promoter strength and gene regulation may hinder the efficient engineering of the strain. to investigate genetic information and complex cellular bioprocesses of c. beijerinckii, an in vivo fluores ... | 2018 | 29158189 |
| butanol fermentation of the brown seaweed laminaria digitata by clostridium beijerinckii dsm-6422. | seaweed represents an abundant, renewable, and fast-growing biomass resource for 3rd generation biofuel production. this study reports an efficient butanol fermentation process carried out by clostridium beijerinckii dsm-6422 using enzymatic hydrolysate of the sugar-rich brown seaweed laminaria digitata harvested from the coast of the danish north sea as substrate. the highest butanol yield (0.42g/g-consumed-substrates) compared to literature was achieved, with a significantly higher butanol:ace ... | 0 | 28432948 |
| effect of ozonolysis parameters on the inhibitory compound generation and on the production of ethanol by pichia stipitis and acetone-butanol-ethanol by clostridium from ozonated and water washed sugarcane bagasse. | sugarcane bagasse (scb) was ozone pretreated and detoxified by water washing, applying a l9(3)(4) orthogonal array (oa) design of experiments to study the effect of pretreatment parameters (moisture content, ozone concentration, ozone/oxygen flow and particle size) on the generation of inhibitory compounds and on the composition of hydrolysates of ozonated-washed samples. ozone concentration resulted the highest influence process parameter on delignification and sugar release after washing; whil ... | 2016 | 27428302 |