| limited proteolysis of type i collagen at hyperreactive sites by class i and ii clostridium histolyticum collagenases: complementary digestion patterns. | the initial proteolytic events in the hydrolysis of rat tendon type i collagen by the class i and ii collagenases from clostridium histolyticum have been investigated at 15 degrees c. sodium dodecyl sulfate-polyacrylamide gel electrophoresis has been used to detect the initial cleavage fragments of both the alpha 1(i) and alpha 2 chains, which migrate at different rates in the buffer system employed. experiments with the class i collagenases indicate that the first cleavage occurs across all thr ... | 1987 | 3032235 |
| purification and properties of an extracellular collagenolytic protease produced by the human oral bacterium bacillus cereus (strain soc 67). | the major collagenolytic proteinase present in the culture filtrate of bacillus cereus (strain soc 67, isolated from the human oral cavity) has been purified to homogeneity by a procedure that comprised concentration of ultrafiltered growth medium on a millipore pttk00005 membrane, precipitation with ammonium sulfate, gel permeation chromatography, chromatofocusing, fast protein liquid chromatography on an anion-exchange column, and finally fast protein liquid chromatography on a gel column. the ... | 1987 | 3040751 |
| enzymatic isolation of cells from neonatal calvaria using two purified enzymes from clostridium histolyticum. | the enzymatic isolation of cells with bacterial collagenase has proved to be a powerful technique for the study of a wide variety of tissues. unfortunately, for some applications such as the isolation of cells from membranous bone, the cellular damage that results from the exposure of the cells to cytotoxic contaminants of bacterial collagenase has limited the usefulness of this approach. the use of chromatographically purified collagenase alone is often ineffective or very slow to release cells ... | 1983 | 6315459 |
| the effects of clostridium histolyticum collagenase on amino sugar-containing compound-collagen complexes. | | 1967 | 4175206 |
| antibody for collagenase from clostridium histolyticum. | | 1965 | 4285539 |
| isolation and ultrastructure of rabbit ovarian mesothelium (surface epithelium). | this study evaluated the efficacy of various dissociating procedures for isolating mesothelial or surface epithelial cells from rabbit ovaries. the procedure which provided the highest and most homogenous cell yield involved the following sequence: (a) incubation of whole ovaries for 60 min in clostridium histolyticum collagenase, type i (300 u/ml); (b) vortexing of ovaries at 30 rpm for 60 s in medium 199; (c) removal of partially detached surface epithelium by gentle scraping with a microdisse ... | 1984 | 6392121 |
| [neutral and basic compounds present in gases produced by clostridium histolyticum, clostridium hastiforme and clostridium ghoni cultured under vacuum in sodium thioglycolate glucose broth]. | the study of gases produced by clostridium histolyticum, c. hastiforme and c. ghoni cultured under vacuum in sodium thioglycolate glucose broth makes it possible to detect compounds not described before for these bacteria, and confirms the production of methane in anaerobic spore-forming bacteria. | 1982 | 6817857 |
| oxygen-dependent metronidazole-resistance of clostridium histolyticum. | sensitivity determinations with 6 strains of clostridium histolyticum showed that the inhibitory action of metronidazole was highly dependent on the oxygen concentration of the environment. under anaerobic conditions they were sensitive but at increased oxygen concentrations moderately sensitive or resistant. the flexible resistance of this aerotolerant anaerobe against metronidazole may interfere with results of sensitivity determinations, estimation of blood levels and it may influence the eff ... | 1981 | 7269847 |
| isolation by affinity chromatography of neutral proteinase from clostridium histolyticum. | | 1973 | 4348177 |
| presence of collagenase from clostridium histolyticum in gingival sulcal debris of a primitive population. | | 1974 | 4358563 |
| the interaction of alpha2-macroglobulin with proteinases. binding and inhibition of mammalian collagenases and other metal proteinases. | 1. experiments were performed to determine whether the specific collagenases and other metal proteinases are bound and inhibited by alpha(2)-macroglobulin, as are endopeptidases of other classes. 2. a specific collagenase from rabbit synovial cells was inhibited by human serum. the inhibition could be attributed entirely to alpha(2)-macroglobulin; alpha(1)-trypsin inhibitor was not inhibitory. alpha(2)-macroglobulin presaturated with trypsin or cathepsin b1 did not inhibit collagenase, and pretr ... | 1974 | 4374931 |
| [proteinases with various substrate specificities in structural studies of yeast cell walls]. | different proteins are revealed in cell wall of yeast cells candida utilis by means of specific proteolysis with subtilisins tv and 72, trypsin and purified collagenase of clostridium histolyticum. some of them were characterized by resistance to trypsin and sensitivity to subtilisin tv. in young cells this group is represented essentially by a protein of 33 kd, which appears to be one of the structural proteins, binding fibrillae of carbohydrate. other proteins proved to be sensitive to both tr ... | 1994 | 7945458 |
| characterization of a human serum inhibitor of clostridium histolyticum proteinase(s). | normal animal sera inhibit at least one clostridium histolyticum proteinase. an assay procedure based on immune hemolysis was developed for the estimation of this inhibition. this inhibitory activity occurs in various levels in the sera of different animal species. the highest titers have been obtained with rat sera. the inhibitory activity from human serum was isolated and purified 16- to 27-fold by sephadex g-200 gel filtration and diethylaminoethyl cellulose or hydroxylapatite chromatography. ... | 1968 | 5303620 |
| substrate specificity of beta-collagenase from clostridium histolyticum. | the substrate specificity of beta-collagenase from clostridium histolyticum has been investigated by measuring the rate of hydrolysis of more than 50 tri-, tetra-, penta-, and hexapeptides covering the p3 to p3' subsites of the substrate. the choice of peptides was patterned after sequences found in the alpha 1 and alpha 2 chains of type i collagen. each peptide contained either a 2-furanacryloyl (fa) or cinnamoyl (cn) group in subsite p2 or the 4-nitrophenylalanine (nph) residue in subsite p1. ... | 1985 | 2982835 |
| purification of nonspecific protease-free collagenase from clostridium histolyticum. | the collagenase (ec 3.4.24.3) produced by the bacterium clostridium histolyticum has been purified free from nonspecific protease contaminants by a two-step procedure. the crude culture medium is chromatographed over heparin-sepharose and sephacryl s-200, and the resulting preparation has no activity versus noncollagenous proteins or n alpha-benzoyl-l-arginine ethyl ester, yet cleaves native thermally reconstituted collagen fibrils quite efficiently (specific activity, 3000 units/mg). the purifi ... | 1985 | 2990251 |
| mode of hydrolysis of collagen-like peptides by class i and class ii clostridium histolyticum collagenases: evidence for both endopeptidase and tripeptidylcarboxypeptidase activities. | the action of three class i (beta, gamma, and eta) and three class ii (delta, epsilon, and zeta) collagenases from clostridium histolyticum on two series of peptides with collagen-like sequences has been examined. the peptides in the first series all contain 4-nitrophenylalanyl-gly-pro-ala in subsites p1 through p3', but each is successively lengthened in the n-terminal direction by addition of an appropriate residue until subsite p5 is occupied. the second group of peptides all have cinnamoyl-l ... | 1985 | 3002446 |
| [interaction of collagenases with certain peptide substrates and inhibitors]. | interactions of collagenases i and ii (clostridiopeptidases) from clostridium histolyticum with hexapeptide substrates in which some l-proline residues are replaced by their d-analogues, as well as with the tripeptide chloromethyl ketone z-gly-pro-gly-ch2cl were studied. a role of stereochemistry of the amino acid residues in the substrate was established and differences between the collagenases, with regard to their specific requirements to substrates, were revealed. the tripeptide chloromethyl ... | 1994 | 8166757 |
| collagen degradation by three clostridium histolyticum collagenase fractions with different substrate specificities. | | 1994 | 8171590 |
| synthesis of n alpha-[3h]acetyl-l-lysine chloromethyl ketone and its use in the fluorographic detection of proteases. | tritiated n alpha-acetyl-l-lysine chloromethyl ketone (alck) was synthesized on a laboratory scale for use as an active-site-directed affinity label in the fluorographic detection of proteases after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the synthesis involved acetylation of n epsilon-benzyloxycarbonyl-l-lysine chloromethyl ketone with [3h]acetic anhydride just before the removal of the benzyloxycarbonyl group. by this method, [3h]alck with a specific activity of 2 ... | 1993 | 8250226 |
| use of specific collagenases for the isolation of rat liver cells with preserved lipase activities. | the heparin-releasable neutral lipase (ec 3.1.1.3) from rat liver is inactivated by the common preparations of collagenases (ec 3.4.24.3) used for the isolation of liver cells. we show that two collagenases purified from clostridium histolyticum allow both the complete preservation of this lipolytic activity and a good viability of liver cells isolated by the usual perfusion protocol. | 1986 | 3010771 |
| peptide substrates for a proteinase of clostridium histolyticum. | | 1968 | 5722284 |
| complement-inactivating proteinase(s) from clostridium histolyticum. | a proteinase fraction inhibiting the hemolytic activity of guinea pig complement was obtained from supernatant fluids of clostridium histolyticum cultures and purified 150- to 350-fold by ammonium sulfate precipitation, sephadex g-75 gel filtration, and diethylaminoethyl cellulose chromatography. an assay was developed based on the inactivation of hemolytic complement. partially purified anticomplementary preparations were active against casein and were capable of "solubilizing" escherichia coli ... | 1968 | 5724966 |
| toxigenicity of clostridium histolyticum. | nishida, shoki (kanazawa university, kanazawa, japan), and masaaki imaizumi. toxigenicity of clostridium histolyticum. j. bacteriol. 91:477-483. 1966.-from 234 soil samples, 21 strains of clostridium histolyticum of different levels of alpha-toxigenicity were isolated by a new method specially designed for the isolation of this species. the alpha-toxigenicity of freshly isolated strains and of stock strains was closely associated with the potentiality for sporulation, growth, and smooth-colony f ... | 1966 | 5935337 |
| [cytologic study of sporulation in clostridium histolyticum. sporogenous strain and sporulation mutants]. | | 1967 | 6065945 |
| active site structure of a hemagglutinating protease from porphyromonas gingivalis: similarity to clostripain. | the active site of a 44-kda hemagglutinating arginine-specific protease from the putative periodontopathogen porphyromonas gingivalis was specifically labeled with n alpha-[3h]acetyllysine chloromethyl ketone. after enzymatic digestion of the labeled enzyme, a labeled active site peptide was isolated by hplc. the sequence of the active site peptide was determined, after its treatment with nabh4 to reduce the ketone group of the reagent moiety, to be asp-val-ala-cys-val-asn-gly. the cysteine resi ... | 1995 | 8595395 |
| purification and separation of individual collagenases of clostridium histolyticum using red dye ligand chromatography. | six collagenases present in the culture filtrate of clostridium histolyticum have been purified to homogeneity. chromatography over hydroxylapatite, sephacryl s-200, and l-arginine-affi-gel 202 removes the brown pigment and the great majority of the contaminating proteinases active against casein, benzoyl-l-arginine ethyl ester, and elastin. reactive red 120 dye ligand chromatography subdivides the collagenases, which have very similar physicochemical properties, among four fractions. the final ... | 1984 | 6087887 |
| characterization of the individual collagenases from clostridium histolyticum. | the six collagenases (alpha, beta, gamma, delta, epsilon, and zeta) from clostridium histolyticum isolated in the preceding paper [bond, m. d., & van wart, h. e. (1984) biochemistry (first paper of three in this issue)] have been characterized in detail. the molecular weights determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis range from 68 000 to 125 000. isoelectric focusing experiments demonstrate that the isoelectric points of the collagenases are in the 5.35-6.20 range. ... | 1984 | 6087888 |
| relationship between the individual collagenases of clostridium histolyticum: evidence for evolution by gene duplication. | the relationship between the six collagenases (alpha, beta, gamma, delta, epsilon, and zeta) isolated and characterized in the preceding papers [bond, m.d., & van wart, h.e. (1984) biochemistry (preceding two papers in this issue)] has been investigated. chemical modification reactions establish that all six enzymes contain essential carboxyl, tyrosine, and lysine residues. circular dichroism spectra of the peptide bond region show that the secondary structures of the collagenases are very simil ... | 1984 | 6087889 |
| purification and characterization of three forms of collagenase from clostridium histolyticum. | three collagenases from clostridium histolyticum, designated c1, c2, and c3, with apparent molecular weights of 96 000, 92 000, and 76 000 were purified. peptide maps of the enzymes prepared by digestion with staphylococcus aureus v-8 protease were found to be similar. cleavage of native c1 with alpha-chymotrypsin or v-8 protease yielded c2 and c3. this suggested that proteolysis of the mr 96 000 collagenase may have occurred in vivo, producing the other two lower molecular weight enzymes. previ ... | 1984 | 6095892 |
| [degradation of collagen by the cariogenic bacteria, streptococcus mutans]. | the behaviour of cariogenic bacteria (streptococcus mutans) is studied with regard to collagen, which represents 90% of the dentine organic matrix. collagenase activity of cariogenic bacteria is measured with radioactive precursors and gel electrophoresis and compared to reference bacterial collagenase (clostridium histolyticum). labelled collagen substrate has been prepared with two different methods: extraction by 0,5 m acetic acid from young rat skin, previously labelled with l-proline 14c, o ... | 1980 | 6248261 |
| circular dichroism comparative studies of two bacterial collagenases and thermolysin. | the recently isolated and purified collagenase produced by achromobacter iophagus, the collagenase from clostridium histolyticum, and thermolysin, three enzymes having common properties, were studied by circular dichroism. from the spectra of the aqueous solutions obtained in the peptide region, the fraction of alpha helix, beta sheet and aperiodic segments in the three proteins could be estimated. good similarity was found between achromobacter collagenase and thermolysin, which both contain a ... | 1980 | 6250633 |
| bacterial collagenases and their clinical applications. | clostridium histolyticum cultures secrete a specific collagenase which can be precipitated from the medium in relatively large amounts. the enzyme is a metalloproteinase capable of cleaving native collagen types i, ii, iii, iv and v. in addition to being a valuable tool in the laboratory the bacterial collagenase has found clinical applications in the treatment of third degree burns and decubitus, diabetic or arterial ulcers. several cases of successful topical use of the crude enzyme in an oint ... | 1982 | 6295413 |
| inhibition of collagenase from clostridium histolyticum by phosphoric and phosphonic amides. | di- and tripeptides with sequences present in collagen that are known to occupy the s1' through s3' subsites at the active site of the collagenase from clostridium histolyticum do not themselves inhibit this zinc protease. thus glycylproline, glycylprolylalanine, and their c-terminal amides are not inhibitors. n alpha-phosphorylglycylproline, n alpha-phosphorylglycyl-l-prolyl-l-alanine, and their c-terminal amides are weak inhibitors with ic50's (concentration causing half-maximal inhibition) of ... | 1983 | 6313042 |
| characterization of the metalloproteinase inhibitor produced by bovine articular chondrocyte cultures. | primary cultures of bovine articular chondrocytes release a latent metalloproteinase which is activated by incubation with organomercurials to degrade proteoglycans. all the enzyme present in the culture medium is latent and binds to columns of heparin-sepharose. the yield of activity from the heparin-sepharose columns (measured after organomercurial treatment) is approximately 300-1000% depending on the chondrocyte culture batch. recombination of column fractions shows that the increase in acti ... | 1983 | 6313063 |
| degradation of basement membrane collagen by proteinases from human gingiva, leukocytes and bacterial plaque. | basement membranes in human gingiva are found in dento-epithelial junction, epithelial-connective tissue junction and endothelium-connective-tissue junction where they have important attachment and filtering functions. the ability of plaque and gingiva-derived proteinases to degrade type iv collagen, the major protein of basement membranes, was examined in vitro. the basement membrane collagen (type iv) isolated from bovine lens capsules was incubated in the presence of enzyme samples. the degra ... | 1983 | 6315910 |
| characterization of a large fragment from annelid cuticle collagen and its relationship to the intact molecule. | digestion of the cuticle collagen from the annelid nereis virens with clostridium histolyticum collagenase yields a native, collagenase-resistant fragment (ccrf) of the molecule with an mr of about 900,000 (kimura and tanzer, j. biol. chem. 252: 8018, 1977). we have produced 940 nm long, sls-crystallites from the collagenase-resistant fragment; the sls pattern matches a region at one end of the 2,400 nm sls obtained from intact cuticle collagen. upon denaturation, the fragment yields two subunit ... | 1983 | 6321095 |
| variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16s rrna-targeted oligonucleotide probes. | six 16s rrna-targeted oligonucleotide probes were designed, validated, and used to quantify predominant groups of anaerobic bacteria in human fecal samples. a set of two probes was specific for species of the bacteroides fragilis group and the species bacteroides distasonis. two others were designed to detect species of the clostridium histolyticum and the clostridium lituseburense groups. another probe was designed for the genera streptococcus and lactococcus, and the final probe was designed f ... | 1998 | 9726880 |
| gene duplication and multiplicity of collagenases in clostridium histolyticum. | clostridium histolyticum collagenase contains a number of different active components. previously we have shown that colh encodes a 116-kda collagenase (colh) and a 98-kda gelatinase. we purified a different 116-kda collagenase (colg) from the culture supernatant and sequenced its gene (colg). we also identified four other gelatinases (105, 82, 78, and 67 kda) and determined their n-terminal amino acid sequences, all of which coincided with that of either colg or colh. hybridization experiments ... | 1999 | 9922257 |
| identification of metal ligands in the clostridium histolyticum colh collagenase. | a clostridium histolyticum 116-kda collagenase has an h415exxh motif but not the third zinc ligand, as found in already characterized zinc metalloproteinases. to identify its catalytic site, we mutated the codons corresponding to the three conserved residues in the motif to other amino acid residues. the mutation affecting his415 or his419 abolished catalytic activity and zinc binding, while that affecting glu416 did the former but not the latter. these results suggest that the motif forms the c ... | 1999 | 10217773 |
| augmentation of permeability in the bronchial epithelium by the house dust mite allergen der p1. | sequence analyses have revealed the existence of homology between certain aeroallergens and proteolytic enzymes. this homology can be expressed functionally, but its significance to airway pathophysiology is unknown. studies with madin-darby canine kidney cells and canine tracheal epithelial cells grown on plastic substrata or matrix proteins suggest that der p1, a major allergen from the house dust mite dermatophagoides pteronyssinus and a cysteine proteinase, or the unfractionated growth mediu ... | 1995 | 7695916 |
| different roles of class i and class ii clostridium histolyticum collagenase in rat pancreatic islet isolation. | crude clostridium histolyticum collagenase was purified by gel filtration and fractionated by anion exchange chromatography into class i with high collagen digestion activity (cda) and low falgpa (2-furanacryloyl-l-leucylglycyl-l-prolyl-l-alanine) hydrolysis activity (fha), class ii with low cda and high fha, and a fraction called class i/ii with intermediate activities. the roles of these collagenase classes in rat pancreatic islet isolation were investigated. dissociations were carried out wit ... | 1995 | 7859945 |
| mediation by bradykinin of rat paw oedema induced by collagenase from clostridium histolyticum. | 1. collagenases are thought to play a major role in the pathology of gas gangrene caused by clostridium histolyticum, because they can destroy the connective tissue barriers. we investigated possible mediators involved in the oedema formation and plasma protein extravasation which follow the injection of a collagenase (ec 3.4.24.3) from clostridium histolyticum into one hind paw of anaesthetized rats. 2. the magnitude of the oedema following a subplantar injection was dependent on the dose of co ... | 1994 | 7915609 |
| cell-associated collagenolytic activity by group b streptococci. | group b streptococci (gbs) are important pathogens in neonatal sepsis, pneumonia, and meningitis. the ability of gbs to invade the collagen-rich amniotic membrane of the placenta has been shown in vitro. in the presence of gbs, the collagen fibrils of the amnion appear disordered, suggesting a role for gbs in premature rupture of membranes. sodium dodecyl sulfate-polyacrylamide gel electrophoresis, sephadex g-200 column chromatography, and gelatin zymograms were used in this study to characteriz ... | 1994 | 7960147 |
| cloning and nucleotide sequence analysis of the colh gene from clostridium histolyticum encoding a collagenase and a gelatinase. | the colh gene encoding a collagenase was cloned from clostridium histolyticum jcm 1403. nucleotide sequencing showed a major open reading frame encoding a 116-kda protein of 1,021 amino acid residues. the deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, hexxh. a 116-kda collagenase and a 98-kda gelatinase were copurified from culture supernatants of c. histolyticum. while the former degraded both native and denatured collagen, the lat ... | 1994 | 7961400 |
| a continuous spectrophotometric assay for clostridium histolyticum collagenase. | | 1981 | 6269461 |
| a trypanosoma cruzi-secreted 80 kda proteinase with specificity for human collagen types i and iv. | specific interactions between parasites and extracellular matrix components are an important mechanism in the dissemination of chagas' disease. binding of the extracellular matrix proteins to trypanosoma cruzi receptors has been described as a significant step in this phenomenon. in this study, a specific proteinase activity was identified in cell-free extracts of amastigote, trypomastigote and epimastigote forms of t. cruzi using the collagenase fluorogenic substrate n-suc-gly-pro-leu-gly-pro-7 ... | 1997 | 9224638 |
| histochemical analysis of the role of class i and class ii clostridium histolyticum collagenase in the degradation of rat pancreatic extracellular matrix for islet isolation. | to understand why class ii clostridium histolyticum collagenase is much more effective than class i in the isolation of rat pancreatic islets, we analyzed the role of these collagenases in pancreatic tissue dissociation. crude collagenase was purified and then fractionated into class i and ii with different enzyme activities and protein compositions. pancreatic tissue was incubated with either class i, class ii, or class i + ii, with or without added protease, under conditions that eliminated en ... | 1997 | 9258514 |
| a study of the collagen-binding domain of a 116-kda clostridium histolyticum collagenase. | the clostridium histolyticum 116-kda collagenase consists of four segments, s1, s2a, s2b, and s3. a 98-kda gelatinase, which can degrade denatured but not native collagen, lacks the c-terminal fragment containing a part of s2b and s3. in this paper we have investigated the function of the c-terminal segments using recombinant proteins. full-length collagenase degraded both native type i collagen and a synthetic substrate, pz-peptide, while an 88-kda protein containing only s1 and s2a (s1s2a) deg ... | 1998 | 9452493 |
| collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain. | the autocrine/paracrine peptide signaling molecules such as growth factors have many promising biologic activities for clinical applications. however, one cannot expect specific therapeutic effects of the factors administered by ordinary drug delivery systems as they have limited target specificity and short half-lives in vivo. to overcome the difficulties in using growth factors as therapeutic agents, we have produced fusion proteins consisting of growth factor moieties and a collagen-binding d ... | 1998 | 9618531 |
| effects of the non-peptide b2 receptor antagonist fr173657 in models of visceral and cutaneous inflammation. | the non-peptide b2 receptor antagonist (e)-3-(6-acetamido-3-pyridyl)-n-[n-[2,4-dichloro-3-[(2-methyl-8-quinolin yl)oxymethyl]phenyl]-n-methylaminocarbonylmethyl]acrylamide (fr173657) was compared to the peptide antagonist icatibant in models of visceral and cutaneous inflammation. | 2000 | 11089906 |
| imaging real-time proteolysis of single collagen i molecules with an atomic force microscope. | the dynamic process of synthesis and degradation of extracellular matrix molecules, including various collagens, is important in normal physiological functions and pathological conditions. existing models of collagen enzymatic degradation reactions are derived from bulk biochemical assays. in this study, we have imaged in real-time individual collagen i molecules and their proteolysis by clostridium histolyticum collagenases in phosphate-buffered saline (pbs) with atomic force microscopy (afm). ... | 1999 | 10433702 |
| the 105-kda protein component of bacillus cereus non-haemolytic enterotoxin (nhe) is a metalloprotease with gelatinolytic and collagenolytic activity. | a sequence of 91 amino acids residues, probably starting from the n-terminal of the mature protein, was determined for the 105-kda protein of the non-haemolytic enterotoxin of bacillus cereus. the last part of this sequence was similar to parts of the n-terminal portions of two collagenases of clostridium histolyticum and clostridium perfringens. zymography, with intact collagen fibril and gelatin as substrates, showed that the 105-kda protein had collagenolytic and gelatinolytic activity. the 1 ... | 1999 | 10499286 |
| metal content and biochemical analyses of a recombinant collagenase prtv from vibrio parahaemolyticus. | prtv is an extracellular metalloprotease of vibrio parahaemolyticus and regarded as a collagenase. inductively coupled plasma-optical emission spectrometry analysis indicated that the recombinant prtv contains 1 mol of zinc per mol of the native enzyme. on the basis of a kinetic study using 2-furanacryloyl-leu-gly-pro-ala (falgpa, the specific substrate for bacterial collagenase) as a substrate, it was suggested that metal ions may play a significant role in the binding and catalytic steps of th ... | 2000 | 11128063 |
| infective endocarditis due to clostridium histolyticum. | | 2000 | 11168053 |
| protease inhibitors. part 7. inhibition of clostridium histolyticum collagenase with sulfonylated derivatives of l-valine hydroxamate. | sulfonylated l-valine hydroxamate derivatives were obtained by reaction of alkyl/arylsulfonyl halides with the title amino acid, followed by treatment with benzyl chloride, and conversion of the cooh moiety to the conhoh group. other derivatives were obtained by reaction of n-benzyl-l-valine with arylisocyanates, arylsulfonylisocyanates or benzoylisothiocyanate, followed by the similar conversion of the cooh into the conhoh moiety, with hydroxylamine in the presence of carbodiimides. the obtaine ... | 2000 | 10699384 |
| protease inhibitors: synthesis of a series of bacterial collagenase inhibitors of the sulfonyl amino acyl hydroxamate type. | a series of sulfonyl amino acyl hydroxamates incorporating alkyl/arylsulfonyl-n-2-nitrobenzyl-l-alanine was prepared. related compounds were obtained by reaction of n-2-nitrobenzyl-l-ala with aryl isocyanates, arylsulfonyl isocyanates, or benzoyl isothiocyanate, followed by the conversion of the cooh into the conhoh moiety. the new compounds were assayed as inhibitors of the clostridium histolyticum collagenase (chc), a bacterial protease involved in the degradation of extracellular matrix. many ... | 2001 | 11405662 |
| oligofructose and long-chain inulin: influence on the gut microbial ecology of rats associated with a human faecal flora. | dietary incorporation of fermentable, indigestible fructans may be of benefit to gastrointestinal health by providing short-chain fatty acids, stimulating the proliferation of bifidobacteria or lactobacilli and suppressing potential pathogenic organisms in the gut. we tested the hypothesis that the effects of fructans on caecal, colonic and faecal short-chain fatty acid concentration and microflora composition depend on their chain length. germ-free rats associated with a human faecal flora were ... | 2001 | 11502244 |
| a simple in vitro method for evaluating the efficacy of different batches of crude clostridium histolyticum collagenase for islet isolation. | | 1995 | 8539957 |
| rapid determination of substrate specificity of clostridium histolyticum beta-collagenase using an immobilized peptide library. | the molecular basis of the substrate specificity of clostridium histolyticum beta-collagenase was investigated using a combinatorial method. an immobilized positional peptide library, which contains 24,000 sequences, was constructed with a 7-hydroxycoumarin-4-propanoyl (cop) fluorescent group attached at the n terminus of each sequence. this immobilized peptide library was incubated with c. histolyticum beta-collagenase, releasing fluorogenic fragments in the solution phase. the relative substra ... | 2002 | 11724807 |
| university of wisconsin solution inhibits the class ii collagenase within crude clostridium histolyticum collagenase. | | 1995 | 8539958 |
| recombinant enzymes for islet isolation: purification of a collagenase from clostridium histolyticum and cloning/expression of the gene. | | 1995 | 8539959 |
| demonstration that 1-trans-epoxysuccinyl-l-leucylamido-(4-guanidino) butane (e-64) is one of the most effective low mr inhibitors of trypsin-catalysed hydrolysis. characterization by kinetic analysis and by energy minimization and molecular dynamics simulation of the e-64-beta-trypsin complex. | 1-trans-epoxysuccinyl-l-leucylamido(4-guanidino)butane (e-64) was shown to inhibit beta-trypsin by a reversible competitive mechanism; this contrasts with the widely held view that e-64 is a class-specific inhibitor of the cysteine proteinases and reports in the literature that it does not inhibit a number of other enzymes including, notably, trypsin. the k1, value (3 x 10(-5) m) determined by kinetic analysis of the hydrolysis of n alpha-benzoyl-l-arginine 4-nitroanilide in tris/hcl buffer, ph ... | 1996 | 8670152 |
| clostripain linker deletion variants yield active enzyme in escherichia coli: a possible function of the linker peptide as intramolecular inhibitor of clostripain automaturation. | the clostripain core protein is composed of the light and heavy chain subunits linked by a nonapeptide into a single polypeptide chain [mol. gen. genet. 240: 140, 1993]. linker removal is due to autocatalytic processing yielding active heterodimeric enzyme. we have expressed mutationally altered core protein variants in the heterologous host escherichia coli to gain further insight into the process of clostripain automaturation. in a mutationally created cys231 --> ser variant, heterodimer forma ... | 1996 | 8875906 |
| protease inhibitors: synthesis of matrix metalloproteinase and bacterial collagenase inhibitors incorporating 5-amino-2-mercapto-1,3,4-thiadiazole zinc binding functions. | matrix metalloproteinase (mmp)/bacterial collagenase inhibitors incorporating 5-amino-2-mercapto-1,3,4-thiadiazole zinc binding functions are reported. a series of compounds was prepared by reaction of arylsulfonyl isocyanates or arylsulfonyl halides with phenylalanyl-alanine, followed by coupling with 5-amino-2-mercapto-1,3,4-thiadiazole in the presence of carbodiimides. these new compounds were assayed as inhibitors of human mmp-1, mmp-2, mmp-8 and mmp-9, and of the collagenase isolated from t ... | 2002 | 12217351 |
| fractions from commercial collagenase preparations: use in enzymic isolation of the islets of langerhans from porcine pancreas. | transplantation of isolated islets of langerhans is an intriguing possibility for the treatment of diabetes mellitus. the isolation of islets from pancreata requires the specific dissociation of the tissue. commercial collagenases from clostridium histolyticum are widely used for this purpose. unfortunately, the effectiveness of these commercial enzymes is not predictable and differs considerably between suppliers and even from lot to lot. this is due mainly to differences in their specific coll ... | 1996 | 8889213 |
| mucosal and invading bacteria in patients with inflammatory bowel disease compared with controls. | endogenous intestinal bacteria and/or specific bacterial pathogens are suspected of being involved in the pathogenesis of inflammatory bowel diseases (ibd). the aim of this study was to investigate ibd tissues for different bacterial population groups harbouring the mucosal surface and/or invading the mucosa. | 2002 | 12374228 |
| pcr detection of clostridium chauvoei in pure cultures and in formalin-fixed, paraffin-embedded tissues. | the polymerase chain reaction (pcr) was used to amplify specific segments of the 16s ribosomal rna gene of clostridium chauvoei, a major pathogen of ruminants. three sets of primers were used to produce amplicons of 159, 836 and 959 base pairs (bp), respectively. the pcr was evaluated by testing clinically important strains of clostridium, including 21 strains of c. chauvoei, five strains each of clostridium septicum and clostridium perfringens and two strains each of clostridium novyi, clostrid ... | 2003 | 12458172 |
| inhibition of collagenase and metalloproteinases by aloins and aloe gel. | the effects of aloe barbadensis gel and aloe gel constituents on the activity of microbial and human metalloproteinases have been investigated. clostridium histolyticum collagenase (chc) results dose-dependently inhibited by aloe gel and the activity-guided fractionation led to an active fraction enriched in phenolics and aloins. aloins have been shown to be able to bind and to inhibit chc reversibly and non-competitively. aloe gel and aloins are also effective inhibitors of stimulated granulocy ... | 2003 | 12479983 |
| heterologous expression of the clostripain gene from clostridium histolyticum in escherichia coli and bacillus subtilis: maturation of the clostripain precursor is coupled with self-activation. | clostripain-specific antibodies were used to analyse the maturation of clostripain prepro-enzyme and core protein heterologously synthesized in escherichia coli and bacillus subtilis. core protein purified from e. coli cells harbouring plasmid phm3-23 underwent calcium-dependent, self-triggered maturation. concomitantly, the inactive form of the enzyme was converted into an active form, demonstrating the self-activation capacity of the clostripain core protein. as judged from western blot analys ... | 1994 | 8025682 |
| the specificity of clostripain from clostridium histolyticum. mapping the s' subsites via acyl transfer to amino acid amides and peptides. | the s' subsite specificity of clostripain from clostridium histolyticum was investigated by acyl transfer to libraries of amino acid amides, ala-xaa dipeptides, proline derivatives and pentapeptides using n alpha-benzoyl-l-arginine ethyl ester as acyl donor. a pentapeptide library consisting of 29 pentapeptides with general structure xaa-ala-ala-ala-gly, ala-xaa-ala-ala-gly and ala-ala-xaa-ala-gly, where xaa represents gly, ala, pro, leu, phe, asp, glu, arg and lys, was prepared by solid-phase s ... | 1994 | 8055964 |
| identification and analysis of a collagenolytic activity in streptococcus mutans. | streptococcus mutans is an important pathogen in coronal caries and is implicated in dental root decay by its ability to bind collagen from various sources. in the present study, electron microscopic analysis demonstrated the ability of s. mutans to bind and to disrupt collagen fibrils of the amniotic membrane. the synthetic peptide falgpa, which is similar in structure to collagen, was degraded by s. mutans, with a lower level of falgpa hydrolytic activity observed in sucrose-grown cells compar ... | 1997 | 8939802 |
| [collagenolytic enzymes synthesized by microorganisms]. | the review uses data obtained by the authors and available in the literature to discuss microbial collagenolytic enzymes, widely employed in scientific research, biotechnology, and medicine. collagenases differing in their structure and the specificity of their action on collagen fibrils were isolated from bacteria (including marine isolates), actinomycetes, and fungi. collagenases produced by clostridium histolyticum and achromobacter iophagus are the best studied enzymes; both are metalloenzym ... | 1996 | 8992239 |
| expression of the colh gene encoding clostridium histolyticum collagenase in bacillus subtilis and its application to enzyme purification. | the colh gene encoding 116-kda collagenase of clostridium histolyticum (ccolh) was cloned into an escherichia coli-bacillus subtilis shuttle vector to develop a method for purification of recombinant collagenase (rcolh). when plasmid pjcm310 containing the colh gene was introduced into b. subtilis db104 and the transformant was grown in lb broth at 37 c, stability of the plasmid was not maintained. however, stability was partly improved by growing the transformant in a modified lb broth containi ... | 1996 | 9013490 |
| involvement of 5-hydroxytryptamine and bradykinin in the hyperalgesia induced in rats by collagenase from clostridium histolyticum. | the involvement of bradykinin, 5-hydroxytryptamine, substance p and prostanoids in the hyperalgesia elicited by collagenase in rat paw was investigated. collagenase (100 micrograms) induced a slight hyperalgesia in kininogen deficient rats in comparison with the behavioural response obtained in normal rats. lisinopril (10(-5) m), and angiotensin-converting enzyme inhibitor, increased the duration of the hyperalgesia elicited in normal rats. ondansetron (0.5 to 5 mumol/kg), a 5-ht3 antagonist, su ... | 1997 | 9151293 |
| a cysteine-activated proteinase of clostridium histolyticum. | | 1952 | 13000153 |
| the partial purification of the lethal toxin of clostridium histolyticum. | | 1952 | 13006410 |
| a comparison of the lethal and hemolytic toxins of clostridium histolyticum. | | 1952 | 13006411 |
| the activity of clostridium histolyticum proteinase on synthetic substrates. | | 1953 | 13031635 |
| the haemolysin of clostridium histolyticum. | | 1953 | 13106224 |
| [studies on the properties of clostridium histolyticum and clostridium septicum antisera]. | | 1953 | 13124941 |
| the heterodimeric protease clostripain from clostridium histolyticum is encoded by a single gene. | clostripain (ec 3.4.22.8) is a heterodimeric cysteine endopeptidase with strict specificity for arg-xaa peptidyl bonds. it is secreted by clostridium histolyticum strains. for the first time we present evidence that both polypeptide chains of native clostripain are encoded by a single gene. dna sequencing of two overlapping genomic dna fragments revealed a single open reading frame (orf) of 1581 nucleotides encoding a polypeptide of 526 amino acid residues. the orf is preceded by canonical trans ... | 1993 | 8341259 |
| [the proteolytic enzymes of clostridium histolyticum; influence of complexon iii and of calcium on the stability of two of these enzymes]. | | 1955 | 13260245 |
| [ammonia production and toxicity of clostridium histolyticum cultured in a gelatinated medium]. | | 1956 | 13363038 |
| pharmacologically-active polypeptide formed from blood globulin by a cysteine-activated protease from clostridium histolyticum. | | 1956 | 13373851 |
| [not available]. | | 1956 | 13395010 |
| exopeptidases of clostridium histolyticum. | | 1957 | 13445228 |
| nutritional studies with clostridium histolyticum. | | 1957 | 13462971 |
| [purification and chemical properties of anatoxin of clostridium histolyticum]. | | 1957 | 13499480 |
| effects of clostridium histolyticum enzymes upon burned and unburned tissues in vitro. | | 1958 | 13521674 |
| new proteolytic enzymes from clostridium histolyticum filtrates. | | 1958 | 13525621 |
| the cysteine-inactivated proteinase (alpha-antigen) of clostridium histolyticum. | | 1958 | 13525622 |
| serological evidence for a specific clostridium histolyticum gelatinase. | | 1958 | 13525623 |
| clostridium histolyticum collagenase: its purification and properties. | | 1958 | 13534676 |
| [studies on clostridium histolyticum anatoxins using zone electrophoresis and counter diffusion on agar]. | | 1959 | 13636341 |
| [study of immunological phenomena by means of tissue culture. iv. cytopathogenic effects of clostridium histolyticum toxin]. | | 1959 | 13658453 |
| studies on the collagenases of clostridium histolyticum. | | 1959 | 13661949 |
| the use of clostridium histolyticum enzymes in the treatment of experimental third degree burns. | | 1959 | 13675991 |
| [research on the odors emitted by anaerobic bacteria. iii. clostridium histolyticum]. | | 1961 | 13701340 |
| [clostripain, a protease from clostridium histolyticum. 2. specificity]. | | 1960 | 13708985 |
| [on proteins and their decomposition products. 18. the hydrolysis of synthetic and natural substrates by collagenase from clostridium histolyticum]. | | 1960 | 13714094 |