| rapid identification of fibronectin, vitronectin, laminin, and collagen cell surface binding proteins on coagulase-negative staphylococci by particle agglutination assays. | seventeen strains of ten different species of coagulase-negative staphylococci were shown to interact with collagen, laminin, fibronectin, and vitronectin immobilized on latex beads. different species of coagulase-negative staphylococci have different capacities to agglutinate proteins. cells of 18 strains of staphylococcus haemolyticus reacted more strongly than did cells of 18 staphylococcus epidermidis strains with proteincoated latex beads, although no significant difference in cell surface ... | 1992 | 1380008 |
| conformational changes in phospholipase a2 upon binding to micellar interfaces in the absence and presence of competitive inhibitors. a 1h and 15n nmr study. | an nmr study has been made of porcine pancreatic phospholipase a2 (pla) in three environments: free in solution, in a binary complex with dodecylphosphocholine micelles, and in a ternary complex with a micelle and the substrate-like inhibitor (r)-1-octyl-2-(n-dodecanoylamino)-2-deoxyglycero-3-phosph oglycol. 1h and 15n chemical shifts, amide exchange rates, and noe intensities are compared for the enzyme in different environments. from these data, structural differences are found for the n-termi ... | 1992 | 1390760 |
| hiv antibodies in babies. | | 1992 | 1392904 |
| post-antibiotic effect of the new streptogramin rp 59500. | the post-antibiotic effect (pae) of rp 59500, a new streptogramin antibiotic, was determined for staphylococcus aureus, staphylococcus epidermidis, staphylococcus haemolyticus, streptococcus pneumoniae, and streptococcus pyogenes. a 30 min exposure of staphylococcus aureus to 5 micrograms/ml of rp 59500 produced a pae of 1.9-6.9 h, and a 60 min exposure of 2.5 micrograms/ml produced a pae of 3.2-8 h. a 30 min exposure of 5 micrograms/ml of rp 59500 of coagulase-negative staphylococci produced a ... | 1992 | 1396777 |
| in-vitro activity of streptogramin rp 59500 against staphylococci, including bactericidal kinetic studies. | the in-vitro activity of rp 59500 and comparative agents was determined against 270 clinical isolates of the genus staphylococcus. mics were performed by micro-dilution dilution. mic90 and mbc90 (mg/l) of rp 59500 were as follows: oxacillin-sensitive staphylococcus aureus (0.5/0.5), oxacillin-resistant s. aureus (0.5/0.5), oxacillin-sensitive staphylococcus epidermidis (0.5/0.5), oxacillin-resistant s. epidermidis (0.25/0.25), oxacillin-resistant staphylococcus hominis (1.0/1.0), oxacillin-resis ... | 1992 | 1399952 |
| in vitro activity of mersacidin (m87-1551), an investigational peptide antibiotic tested against gram-positive bloodstream isolates. | we measured the in vitro activity of mersacidin (formerly m87-1551) against 183 clinical isolates (vancomycin susceptible) and 12 additional vancomycin-resistant strains of gram-positive bacteria. the activity for mersacidin increased an average twofold (range, 1.7- to 7.6-fold) in a calcium-enriched medium. the minimum inhibitory concentration (mic)90 for mersacidin was 8-32 times higher than vancomycin for staphylococci, 4-64 times higher for enterococci, and up to 32 times higher for other or ... | 1992 | 1424522 |
| identification of calcium-dependent phospholipid-binding proteins (annexins) from guinea pig alveolar type ii cells. | a new group of calcium-regulating proteins, called annexins or ca(++)-dependent phospholipid-binding proteins (plbp), have been detected in different species, organs and cell types. in the present study, we have identified and quantitated plbp from guinea pig lung, lavage fluid and alveolar type ii cells to elucidate the possible role of plbp in lung surfactant biogenesis and secretion. lungs were lavaged and type ii cells from lavaged lung were isolated by elastase digestion and purified by cen ... | 1992 | 1435768 |
| phage pattern and antibiotic resistance pattern of coagulase-negative staphylococci obtained from immunocompromised patients. | a total of 152 coagulase-negative staphylococcal strains were isolated from clinical samples of 14 patients hospitalized after bone-marrow transplantation in a specialized hospital ward in hungary, during an 18-month period between 1987 and 1989. two species, staphylococcus epidermidis and staphylococcus haemolyticus, predominated (each, 45%). using pulverer and co-workers' phage set for typing, 68% of the isolates were typable; 16 phage patterns were observed. a characteristic long pattern with ... | 1992 | 1461152 |
| in vitro adherence of staphylococcus saprophyticus, staphylococcus epidermidis, staphylococcus haemolyticus, and staphylococcus aureus to human ureter. | staphylococcus saprophyticus adhered to human ureteral epithelium in vitro. the levels of adherence, which were determined quantitatively with the scanning electron microscope, correlated well with bacterial hemagglutinating activities with sheep erythrocytes (r = 0.9459, p < 0.01). transmission electron microscopy revealed that the adhering bacteria and the hemagglutinating bacteria possessed similar pili-like structures on their cell surfaces. staphylococcus epidermidis, staphylococcus haemoly ... | 1992 | 1462477 |
| dielectric properties of human glioma and surrounding tissue. | dielectric properties of human glioma and surrounding tissue from five patients were measured. experiments were performed at frequencies of 5-500 mhz at 24 +/- 0.5 degrees c using a network analyser and a coaxial line capacitive sensor. the permittivity and conductivity of tumours were 30% higher than that of the surrounding tissues due to their higher water content. the characteristic of less differentiation in tumours clearly was reflected in the dielectric properties, namely a smaller paramet ... | 1992 | 1479201 |
| experimental podophyllotoxin (bajiaolian) poisoning: ii. effects on the liver, intestine, kidney, pancreas and testis. | young male rats were orally intubated with podophyllotoxin: group i, control animals, orally fed with vehicle only; group ii, fed with an initial dose of 5 mg.kg-1 b.w., followed by a daily dose of 1.67 mg.kg-1 b.w. for 7d. group iii, fed with an initial dose of 15 mg.kg-1 b.w., followed by a daily dose of 5 mg.kg-1 b.w. for 7d. all animals were sacrificed 72 h after the last dosing. histopathological examination revealed dose-related fatty change of the liver, atrophy and degenerative changes o ... | 1992 | 1489523 |
| [multi-resistant strains of salmonella typhi in spain]. | most salmonella typhi isolated in spain are susceptible to antibiotics commonly used in its treatment as chloramphenicol, ampicillin and cotrimoxazole. three multiresistant strains have been isolated from different patients the last two years. two phage type m1, biotype xylose tetrationate + strains were isolated from blood of two patients in bembibre (león). one phage type e1a biotype xylose + tetrationate reductase + strain was isolated from blood and faeces of one patient in barcelona. all st ... | 1992 | 1489795 |
| in vitro activity of ro 23-9424, a dual-action antibacterial agent, against bacterial isolates from cancer patients compared with those of other agents. | the in vitro activity of ro 23-9424 against bacterial isolates from patients with cancer was compared with those of fleroxacin, ciprofloxacin, cefoperazone, and ceftazidime. ro 23-9424 inhibited the majority of the members of the family enterobacteriaceae and all aeromonas isolates at a concentration of less than or equal to 1.0 micrograms/ml. it was also active against acinetobacter spp. and haemophilus influenzae, including beta-lactamase-producing strains. the mic for 90% of isolates (mic90) ... | 1992 | 1503453 |
| in-vitro activities of 14-, 15- and 16-membered macrolides against gram-positive cocci. | the in-vitro activities of the 14-membered macrolides erythromycin, dirithromycin, roxithromycin, clarithromycin, the 15-membered compound azithromycin and the 16-membered macrolides (16 mm) josamycin, spiramycin and midecamycin acetate (mom) have been compared against staphylococci, enterococci and streptococci. results have been analysed separately according to the sensitivity status of the tested strains to erythromycin, namely sensitive (s), inducibly resistant (ir) or constitutively resista ... | 1992 | 1506329 |
| in vitro activities of three semisynthetic amide derivatives of teicoplanin, mdl 62208, mdl 62211, and mdl 62873. | mdl 62208, mdl 62211, and mdl 62873 are three semisynthetic amide derivatives of teicoplanin (mdl 62208 is an amide of teicoplanin aglycone, mdl 62211 is an amide of the teicoplanin a2 complex, and mdl 62873 is the corresponding derivative of peak a2-2 of the complex). the three semisynthetic glycopeptides were evaluated for in vitro antibacterial activity in comparison with the parent drug (teicoplanin) and vancomycin. a variety of gram-positive bacteria of clinical origin, whose species were c ... | 1992 | 1534977 |
| comparative therapeutic efficacy of teicoplanin and vancomycin in normal and in neutropenic mice infected with staphylococcus haemolyticus. | | 1992 | 1535069 |
| an endemic strain of staphylococcus haemolyticus colonizing and causing bacteremia in neonatal intensive care unit patients. | coagulase-negative staphylococci are now the major cause of bacteremia in neonatal intensive care units. to date, coagulase-negative staphylococci causing neonatal infections have been found to be distinct when typed by standard techniques. to determine whether or not an endemic strain could be identified using more discriminatory techniques, we characterized coagulase-negative staphylococci isolates obtained from a prospective study of coagulase-negative staphylococci bacteremia in a neonatal i ... | 1992 | 1557264 |
| rat urate oxidase produced by recombinant baculovirus expression: formation of peroxisome crystalloid core-like structures. | urate oxidase (ec 1.7.3.3), which catalyzes the oxidation of uric acid to allantoin, is present in most mammals but absent in humans and hominoid primates. in rats and most other mammals that catabolize uric acid to allantoin, this enzyme is localized within the crystalloid cores of peroxisomes present in liver parenchymal cells. to determine whether urate oxidase forms these crystalloid cores or whether core-forming protein(s) exist in association with urate oxidase, a baculovirus expression ve ... | 1992 | 1594592 |
| a dna-probe for the detection of the species staphylococcus haemolyticus. | a 1.3-kb dna fragment isolated from staphylococcus haemolyticus strain dsm 20264 can be used as a specific probe for this species. the probe hybridized with 39 clinical isolates of s. haemolyticus but not with any of the 121 isolates representative of the other 25 species of staphylococci described to date. | 1992 | 1612422 |
| [study of the virulence of coagulase-negative staphylococci in experimental infections]. | the pathogenicity and virulence of 3 strains of staphylococcus epidermidis, staphylococcus haemolyticus and staphylococcus saprophyticus, respectively were studied in balb/c mice by intraperitoneal (ip) challenge using 4 bacterial suspensions of different colony forming units (cfu) of each strain. strains were isolated from wound, blood, and urine of inpatients. on the base of the lethality rates, s. saprophyticus proved to be the most virulent (ld50 = 2.7-2.9 x 10(7) cfu/g body wt), while the s ... | 1992 | 1625850 |
| [a staphylococcus haemolyticus study in urinary infections. an analysis of 8 cases]. | urinary infections caused by staphylococcus are attributed usually to staphylococcus epidermidis or staphylococcus saprophyticus. 8 cases of urinary infection due to s. haemolyticus are discussed: 5 adults all of them over 66 years, diagnosed respectively of diabetes, cancer and stroke. three children diagnosed of febrile syndrome, encephalopathy and enterocolitis associated with urinary infection. s. haemolyticus was identified through the determination of 19 biochemical parameters. the antibio ... | 1992 | 1626088 |
| molecular epidemiology of staphylococcus haemolyticus strains isolated in an albanian hospital. | a recent outbreak of erythroderma in young children in an albanian hospital was investigated. the etiology was not established, but staphylococcus haemolyticus was frequently isolated from the affected children and from staff working in the same unit. possible relationships among the isolates were investigated by using classical techniques (biotype, antimicrobial susceptibility, and extrachromosomal dna pattern) and by restriction endonuclease analysis (rea) of total dna. control isolates of pro ... | 1991 | 1653266 |
| observations on coagulase-negative staphylococci in a neonatal unit in india. | fifty neonates were included in a prospective study which determined the frequency of colonization and infection with coagulase-negative staphylococci (cns) in a neonatal unit. the isolates were characterised by species, antibiotic resistance and ability to produce slime. twenty-four epidemiologically related isolates (including four isolates from the hands of staff) were also phage typed and had plasmid analysis performed. on the first day of birth, 18% of infants were colonized with cns, 40% a ... | 1991 | 1686040 |
| homology of meca gene in methicillin-resistant staphylococcus haemolyticus and staphylococcus simulans to that of staphylococcus aureus. | a penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant staphylococcus haemolyticus and staphylococcus simulans. dna from these strains hybridized to the meca gene from staphylococcus aureus; however, the chromosomal hindiii fragments containing the meca genes were 3.4 kilobases in s. haemolyticus and 4.3 kilobases in s. simulans. | 1990 | 1691614 |
| detection of cytomegalovirus in lung allografts. comparison of histologic and immunohistochemical findings. | although the histologic manifestation of cytomegalovirus (cmv) is usually characteristic intracellular inclusions and cytomegaly, some investigators, using immunohistochemical or in situ hybridization techniques, have demonstrated the presence of histologically occult infections in certain tissues. a series of lung biopsy specimens from pulmonary transplant recipients were studied using a monoclonal antibody (cch2) to cmv early viral antigen and the results were compared with routine histologic ... | 1991 | 1720923 |
| detection of a soluble acrosome reaction-inducing factor, different from serum albumin, associated with the ovulated egg-cumulus complex. | soluble extracts of the ovulated hamster egg-cumulus complex (ecc) were tested on capacitated sperm for activity in inducing the physiological acrosome reaction (ar). evidence for occurrence of the physiological ar included enhanced sperm penetration of intact homologous zonae pellucidae as well as induction of ar in nonattached and in zona-bound sperm following a brief coincubation with test compound. since hamster serum albumin, a major protein of hamster body fluids, also induces spontaneous ... | 1991 | 1751045 |
| susceptibility of staphylococcus species and subspecies to fleroxacin. | twenty-four staphylococcus species or subspecies were examined for their susceptibilities to the fluoroquinolone fleroxacin (ro 23-6240) by disk diffusion (5-micrograms disk) and by agar dilution for the determination of mics. resistant strains were further tested for their susceptibilities to oxacillin and the fluoroquinolone ciprofloxacin. reference strains of the novobiocin-resistant species (staphylococcus saprophyticus, staphylococcus cohnii, staphylococcus xylosus, staphylococcus arlettae, ... | 1991 | 1759838 |
| repletion and depletion of vitamin e in gastroduodenal mucosa. | male rats were used to assess the effect of fasting and depletion and repletion rates on the levels of vitamin e in the liver and in mucosa from the fundus, antrum, and duodenum. fasting for 24 h did not produce a significant decrease in vitamin e content in gastroduodenal mucosa. feeding rats a diet low in vitamin e content for 10 weeks produced very low levels of the vitamin in all the tissues studied (below 2.4 micrograms/g tissue). feeding rats a diet high in vitamin e content for 10 weeks p ... | 1991 | 1771388 |
| in vitro selection of resistance to vancomycin in bloodstream isolates of staphylococcus haemolyticus and staphylococcus epidermidis. | the purpose of the study was to determine whether vancomycin-resistant strains of staphylococcus haemolyticus could be selected regardless of the initial mic of vancomycin. twenty-one bloodstream isolates of staphylococcus haemolyticus were studied by broth and agar selection methods. the broth method selected strains for which mics of vancomycin ranged from 4 to 32 micrograms/ml and mbcs from 16 to greater than 128 micrograms/ml. the agar method selected strains for which mics ranged from 8 to ... | 1991 | 1802693 |
| comparative in vitro activity of teicoplanin and vancomycin against united states teicoplanin clinical trial isolates of gram-positive cocci. | in this study, the in vitro activity of teicoplanin and vancomycin was directly compared against 503 gram-positive cocci isolated during the u.s. teicoplanin clinical trials. both antibiotics were equally active against oxacillin-sensitive staphylococcus aureus, oxacillin-sensitive and oxacillin-resistant staphylococcus epidermidis, and other coagulase-negative staphylococci, except staphylococcus haemolyticus. teicoplanin was fourfold more active than vancomycin against oxacillin-resistant s. a ... | 1991 | 1826479 |
| activities of two new teicoplanin amide derivatives (mdl 62211 and mdl 62873) compared with activities of teicoplanin and vancomycin against 800 recent staphylococcal isolates from france and the united states. | mdl 62211 is the amide derivative of the teicoplanin complex and mdl 62873 is a more focused amide derivative of the teicoplanin a2-2 peak. each investigational compound had nearly identical activity and was 2- to 16-fold more active than teicoplanin or vancomycin. the mdl 62873 mics for 90% of the strains tested were as follows: staphylococcus aureus, oxacillin susceptible, 0.12 micrograms/ml; s. aureus, oxacillin resistant, 0.25 micrograms/ml; coagulase-negative staphylococci (cns), oxacillin ... | 1991 | 1828137 |
| susceptibility of staphylococcus species and subspecies to teicoplanin. | twenty-four staphylococcus species and their subspecies were examined for their susceptibilities to teicoplanin by disk diffusion (30-micrograms disk) and agar dilution for the determination of mics. moderately susceptible and resistant clinical strains were further tested for their susceptibilities to oxacillin and vancomycin. teicoplanin resistance was not observed in the reference strains of the various staphylococcus species isolated from healthy volunteers or animals. however, the novobioci ... | 1991 | 1835340 |
| comparative therapeutic efficacy of teicoplanin and vancomycin in normal and in neutropenic mice infected with staphylococcus haemolyticus. | this study describes the evaluation of a murine bacteraemia model for assessing antibiotic efficacy in normal and neutropenic mice infected with coagulase-negative staphylococci. in one such evaluation, it was found that there was no significant (p greater than 0.05) difference in the ability of teicoplanin or vancomycin to protect normal or neutropenic cd-1 mice, lethally-infected with staphylococcus haemolyticus. however, about a four-fold increase of either antibiotic was needed to protect th ... | 1991 | 1838105 |
| presence and localization of a 30-kda basic fibroblast growth factor-like protein in rodent testes. | we have used a recently characterized rabbit antiserum against basic fibroblast growth factor (bfgf), which recognizes various forms of bfgf, to examine the presence and localization of bfgf in the testes of adult rats and mice and the 5-day-old rat. in western blots of testicular homogenates of adult rats and mice and immature rats, immunoreactive single bands at approximately 30 kda were detected. immunocytochemistry revealed specific staining restricted to the tubular compartment. in 5-day-ol ... | 1991 | 1855483 |
| optimal conditions for long-term photoreceptor cell rescue in rcs rats: the necessity for healthy rpe transplants. | retinal pigment epithelial (rpe) cells from normal long-evans, healthy royal college of surgeons (rcs) pigmented congenic (rdy+p+) and rcs pigmented dystrophic rats were transplanted into retinas of rcs non-pigmented dystrophic rats at post-natal days 17 and 26 (p17 and p26). when examined at p60, rescued photoreceptor cells were found in all transplanted groups. in addition, a small, but significant increase in the outer nuclear layer (onl) thickness was detected in retinas injected with vehicl ... | 1991 | 1855542 |
| bacterial formation of n-nitroso compounds in the rat stomach after omeprazole-induced achlorhydria. | n-nitrosamine formation by bacteria in the achlorhydric stomach has been proposed as an important factor in the development of gastric cancer. thus, the effect of the presence of bacteria in the stomach on endogenous nitrosation was investigated in rats given omeprazole (an inhibitor of gastric h+, k((+)-atpase) which reduces gastric secretion sufficiently to allow survival of a bacterial suspension of escherichia coli or pseudomonas. when rats were given both thiazolidine 4-carboxylic acid and ... | 1991 | 1855848 |
| infectious crystalline keratopathy caused by candida albicans. | two patients developed corneal opacities resembling infectious crystalline keratopathy. predisposing factors included a recent corneal transplant with suture replacement in one patient and postradiation keratoconjunctivitis with disposable therapeutic contact-lens wear in the other patient. both patients were using a topically applied corticosteroid and an aminoglycoside antimicrobial. smears of corneal scrapings showed numerous yeasts without inflammatory cells. culturing yielded candida albica ... | 1991 | 1882943 |
| antimicrobial activity of cefpirome. an update compared to five third-generation cephalosporins against nearly 6000 recent clinical isolates from five medical centers. | cefpirome, cefotaxime, ceftazidime, cefoperazone, ceftizoxime, and ceftriaxone were tested against approximately 6000 fresh clinical isolates from five medical centers. for 3031 strains of enterobacteriaceae tested, cefpirome consistently had the lowest mic50s and lowest percentage of resistant strains. cefpirome was also the most active agent against the 2138 gram-positive cocci tested; staphylococcus haemolyticus was uniformly resistant to all agents tested. against 791 nonenteric gram-negativ ... | 1991 | 1889187 |
| relative quantification of collagen mrna in fibroblasts by a radioactive polymerase chain reaction technique. | a radioactive polymerase chain reaction (pcr) method has been developed for the relative quantification of the human alpha-2 chain of type i collagen [hu alpha-2(i)] in cells. cdnas generated by reverse transcription from the total pool of cytoplasmic rna serve as a template for polymerase chain reaction amplification of a hu alpha-2(i) cdna primed by two sequence-specific synthetic oligonucleotides. the distinctive 390 bp hu alpha-2(i) cdna and two aval fragments of 220 and 170 bp are identifie ... | 1991 | 1890540 |
| submicromolar manganese dependence of golgi vesicular galactosyltransferase (lactose synthetase). | 1. manganese(ii) buffers were set up with inorganic triphosphate, trimethylenediaminetetraacetate and tetramethylenediaminetetraacetate to study the mn dependence of beta 1,4-galactosyltransferase (lactose synthetase) in preparations of rat mammary gland. 2. in intact particulate preparations, treated with the calcium ionophore a23187, lactose synthesis was abolished by chelators and restored by bivalent transition metal ions in a manner characteristic of activation site i of the pure enzyme. ni ... | 1991 | 1899383 |
| molecular cloning, purification, and properties of a plasmid-encoded chloramphenicol acetyltransferase from staphylococcus haemolyticus. | a small chloramphenicol resistance (cmr) plasmid of approximately 3.75 kb, designated pscs5, was isolated from staphylococcus haemolyticus. this plasmid encoded an inducible chloramphenicol acetyltransferase (cat; ec 2.3.1.28). the cat gene of pscs5 was cloned into the escherichia coli plasmid vector pbluescript skii+. it differed in its nucleotide sequence and deduced amino acid sequence from the cat genes described previously in staphylococci and other gram-positive bacteria. the cat enzyme wa ... | 1991 | 1929282 |
| nucleotide sequence and phylogeny of a chloramphenicol acetyltransferase encoded by the plasmid pscs7 from staphylococcus aureus. | the nucleotide sequence of the chloramphenicol acetyltransferase gene (cat) and its regulatory region, encoded by the plasmid pscs7 from staphylococcus aureus, was determined. the structural cat gene encoded a protein of 209 amino acids, which represented one monomer of the enzyme chloramphenicol acetyltransferase (cat). comparisons between the amino acid sequences of the pscs7-encoded cat from s. aureus and the previously sequenced cat variants from s. aureus, staphylococcus intermedius, staphy ... | 1991 | 1929326 |
| enhanced healing of 30 microns gore-tex ptfe microarterial prostheses by alcohol-pretreatment. | polytetrafluoroethylene (ptfe) microvascular prostheses with a fibril length of 30 microns were pretreated with alcohol (n = 18), implanted into the abdominal aorta of rats and were evaluated at 1 day (n = 3), 1 week (n = 3), 3 weeks (n = 6) and 6 weeks (n = 6) to determine whether alcohol-pretreatment might improve their healing. untreated ptfe microvascular prostheses (n = 18) functioned as controls (all prostheses: length 10 mm, i.d. 1.5 mm). the alcohol-pretreated ptfe microarterial prosthes ... | 1991 | 1933114 |
| identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. | a simple and reliable method using a polymerase chain reaction (pcr) was devised to identify methicillin-resistant staphylococci. by using lysates of the strain to be tested as templates and 22-mer oligonucleotides as primers, a 533-bp region of meca, the structural gene of a low-affinity penicillin-binding protein (pbp 2'), was amplified by pcr and detected by agarose gel electrophoresis. results obtained by this method were compared with those obtained by broth microdilution mic determination ... | 1991 | 1939577 |
| neither heparin nor acetylsalicylic acid influence the clinical course in human plasmodium falciparum malaria: a prospective randomized study. | procoagulant alterations and thrombocytopenia in falciparum malaria correlate with parasitemia, serum levels of tumor necrosis factor alpha (tnf alpha), and clinical severity. thus, heparin or acetylsalicylic acid (asa), which are used frequently to prevent thrombosis and (in the case of asa) to control fever, could be potentially beneficial. we randomized 97 patients with falciparum malaria into three groups: 33 patients received low-dose heparin subcutaneously, 31 received asa intravenously, a ... | 1991 | 1951871 |
| the effect of prophylaxis with chloroquine and proguanil on delayed-type hypersensitivity and antibody production following vaccination with diphtheria, tetanus, polio, and pneumococcal vaccines. | in vitro studies have shown that anti-malarial drugs suppress immunity. in this study, the effects of chloroquine and proguanil (paludrine) on the cellular and humoral immune system were measured by two in vivo methods: 1) cell-mediated immunity (delayed cutaneous hypersensitivity) i.e., skin tests with seven delayed-type common antigens (multitest) and 2) humoral immunity by measurement of specific antibody response to vaccination. sixty healthy young individuals were randomized into four group ... | 1991 | 1951872 |
| evaluation of restriction endonuclease fingerprinting of chromosomal dna and plasmid profile analysis for characterization of multiresistant coagulase-negative staphylococci in bacteremic neonates. | a procedure was developed for restriction endonuclease fingerprinting (ref) of the chromosomal dna of coagulase-negative staphylococci. a total of 48 isolates comprising 29 staphylococcus epidermidis and 19 staphylococcus haemolyticus isolates from blood and mucocutaneous sites of 15 premature neonates were characterized by ref, plasmid profile (pp) analysis, antimicrobial susceptibility testing, biotyping, and slime production. on the basis of ref analysis of chromosomal dna, the 48 coagulase-n ... | 1990 | 1968906 |
| evaluation of the staph-zym system with staphylococci isolated from bovine intramammary infections. | a total of 148 staphylococci isolated from bovine intramammary infections were used to evaluate the staph-zym system (rosco, taastrup, denmark). the overall accuracy of the system was 91.9%. the system correctly identified all strains of staphylococcus aureus, staphylococcus simulans, and staphylococcus xylosus and 95% of staphylococcus intermedius strains. of 33 staphylococcus hyicus strains, 31 (93.9%) were classified correctly by the staph-zym system, as well as 8 (80%) of 10 staphylococcus c ... | 1991 | 1993769 |
| epidemiology of campylobacter enteritis. | campylobacter enteritis is the commonest form of infective diarrhoea in most developed countries of the world. in england and wales laboratory reports indicate an annual incidence of about 85/100,000, but the true rate is probably nearer 1100/100,000. measured costs run to many millions of pounds per year. most infections are sporadic and believed to be foodborne; large outbreaks are infrequent and mostly due to the consumption of raw milk or unchlorinated water. raw meats and animal products, n ... | 1991 | 2018709 |
| occurrence of staphylococcus lugdunensis in consecutive clinical cultures and relationship of isolation to infection. | consecutive record review over a 63-month period revealed 229 staphylococcus lugdunensis isolates, or 10.1% of the staphylococcal species that were not staphylococcus aureus or staphylococcus epidermidis. a total of 155 s. lugdunensis specimens were isolated from sites over the entire bodies of the 143 patients studied. the most common clinical diagnoses were skin and skin structure infections (55.4%) and blood and vascular catheter infections (17.4%). for 40% of the reviewed specimens, s. lugdu ... | 1991 | 2037657 |
| a convenient method for differentiation of coagulase-negative staphylococci isolated from bovine mammary glands. | the utility of trehalose-mannitol broth and arabinose-cellobiose broth for identification of staphylococcus epidermidis and novobiocin-resistant staphylococci was determined using 236 coagulase-negative staphylococci isolated from bovine mammary glands. none of the 49 s. epidermidis strains was positive in trehalose-mannitol broth; whereas, all strains of staphylococcus hyicus, staphylococcus chromogenes, staphylococcus haemolyticus, staphylococcus hominis, staphylococcus warneri, and staphyloco ... | 1991 | 2045549 |
| comparative study of hemolytic substances produced by coagulase-negative staphylococcus strains. | hemolytic substances h7, h62, and e56, produced by staphylococcus haemolyticus 7 and 62 and s. epidermidis 56, respectively, were purified. h7 and h62 are probably similar on the basis of their isoelectric focusing profiles in 8 m urea and complete immunological identity as revealed by immunodiffusion with rabbit anti-h7 and anti-e56 sera. for e56, we observed seven bands instead of three in isoelectric focusing and only partial immunological identity with h7 and h62. however, h7 and e56 were si ... | 1990 | 2114358 |
| failure of teicoplanin therapy in two neutropenic patients with staphylococcal septicemia who recovered after administration of vancomycin. | a report is given on two neutropenic patients with staphylococcal septicemia caused by staphylococcus haemolyticus and staphylococcus aureus (both strains methicillin-resistant) who failed to respond to therapy with teicoplanin. both strains were resistant to teicoplanin (mic 16 and 8 mg/l respectively), but remained sensitive to vancomycin (mic 2 and 4 mg/l respectively). replacement of teicoplanin with vancomycin led to full recovery of both patients and their discharge from hospital. these tw ... | 1990 | 2138543 |
| selection of vancomycin- and teicoplanin-resistant staphylococcus haemolyticus during teicoplanin treatment of s. epidermidis infection. | | 1990 | 2140112 |
| phage-typing of coagulase-negative staphylococci. factors influencing typability. | factors influencing the phage-typability of coagulase-negative micrococcaceae have been studied in 2,778 clinical isolates comprising a) 209 consecutive isolates from one laboratory, b) 2,107 clinical strains submitted for phage-typing for epidemiological reasons, and c) 462 strains representing all isolates of presumed clinical significance found in two laboratories during one month. the reproducibility was acceptable at duplicate repeated typing of the same strains as well as by typing epidemi ... | 1990 | 2141261 |
| [the use of teicoplanin in neutropenic patients: values and limits]. | the efficacy and the safety of teicoplanin were evaluated for the treatment of febrile episodes in neutropenic patients. a total of 18 patients received teicoplanin once daily as an intravenous injection of 200 mg in 6 patients (after a loading dose of 400 mg) or 400 mg in 12 patients (loading dose: 800 mg). the mean duration of therapy was 15.9 days (range 9 to 39 days). in all patients teicoplanin was combined with another antibiotic usually a beta-lactam. thirteen of 18 patients were successf ... | 1990 | 2143573 |
| in vitro activity of ly264826, a new glycopeptide antibiotic, against gram-positive bacteria isolated from patients with cancer. | the in vitro activity of ly264826, a novel glycopeptide antibiotic produced by amycolatopsis orientalis, was compared with those of vancomycin, teicoplanin, and oxacillin against 311 gram-positive clinical isolates from patients with cancer, ly264826 had lower mics for 90% of isolates (mic90) than vancomycin for all species tested. it was active against oxacillin-resistant isolates including staphylococcus aureus (mic90, 0.5 micrograms/ml), staphylococcus haemolyticus (mic90, 2.0 micrograms/ml), ... | 1990 | 2149921 |
| sulfation of tyrosine residues in coagulation factor v. | sulfation of human coagulation factor v was investigated by biosynthetically labeling the products of hepg2 cells with [35s]sulfate. there was abundant incorporation of the sulfate label into a product identified as factor v by immunoprecipitation, lability to proteases, affinity for the lectin jacalin, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. two or more sites in factor v incorporated sulfate as indicated by labeling of different peptide chains of factor va. the 150-kd act ... | 1990 | 2168225 |
| collagens synthesized by healing fractures. | several extracellular matrices are formed by healing fractures, and the collagens within these matrices have been identified in rabbit and rat fracture models. type iii collagen is the major collagen of the fibrous matrix that forms along the periosteal surface. type i collagen is secreted in large amounts as trabeculae of bone develop within the fibrous tissue. type v collagen is found in both the fibrous tissue and bone; it is particularly associated with blood vessels. type ii collagen is the ... | 1990 | 2189630 |
| ventilatory management of ards: can it affect the outcome? | animal studies have demonstrated that mechanical ventilation with high peak inspiratory pressure (pip) results in acute lung injury characterised by hyaline membranes, granulocyte infiltration and increased pulmonary and systemic vascular permeability. this can result in progressive respiratory failure and death. in surfactant deficient lungs this occurs with tidal volumes (vt) as low as 12 ml/kg, and pip as low as 25 cm h2o, values which are frequently used clinically. the mechanisms resulting ... | 1990 | 2193041 |
| characterization of the cdna coding for mouse prothrombin and localization of the gene on mouse chromosome 2. | a series of overlapping cdnas coding for mouse prothrombin (coagulation factor ii) have been isolated and the composite dna sequence has been determined. the complete prothrombin cdna is 1,987 bp in length [excluding the poly(a) tail] and codes for 18 bp of 5' untranslated sequence, an open reading frame coding for 618 amino acids, a stop codon, and a 3' untranslated region of 112 bp followed by a poly(a) tail. the translated amino acid sequence predicts a molecular weight of 66,087, which inclu ... | 1990 | 2222810 |
| trends in canadian tobacco consumption, 1980-89. | | 1990 | 2224718 |
| trends in canadian tobacco consumption, 1980-89. | | 1990 | 2224718 |
| comparative in vitro activity of cefpirome and cefepime, two new cephalosporins. | in in vitro tests the broad-spectrum cephalosporins cefpirome and cefepime were highly active against enterobacteriaceae, although often less so against strains resistant to amoxicillin-clavulanate and ticarcillin-clavulanate, and against most strains of acinetobacter spp. and aeromonas hydrophila. they were also active against pseudomonas aeruginosa, although strains with non-plasmid mediated beta-lactam resistance were sometimes less sensitive. other pseudomonas spp. varied in their sensitivit ... | 1990 | 2226498 |
| vancomycin resistance in staphylococcus haemolyticus causing colonization and bloodstream infection. | the increase in the incidence of infections due to beta-lactam-resistant coagulase-negative staphylococci has resulted in expanded use of vancomycin for such infections. despite this, coagulase-negative staphylococci have remained susceptible to vancomycin in recent years. this report describes a strain of staphylococcus haemolyticus with increased resistance to vancomycin (mic, 8.0 to 16 micrograms/ml). s. haemolyticus was initially isolated from a patient with acute leukemia and neutropenia in ... | 1990 | 2229388 |
| neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats. | intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ... | 1990 | 2245800 |
| neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats. | intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ... | 1990 | 2245800 |
| tardive eating dystonia. | after cessation of long-term treatment with haloperidol, a 77-year-old man developed severe dyskinetic-dystonic movements involving mainly the tongue and associated also with oromandibular dystonia and blepharospasm that were manifested exclusively during the process of eating and that interfered with feeding. there were no spontaneous involuntary movements nor were they apparent during any other simple or complex activations of the orofacial musculature. | 1990 | 2259358 |
| presence of an additional penicillin-binding protein in methicillin-resistant staphylococcus epidermidis, staphylococcus haemolyticus, staphylococcus hominis, and staphylococcus simulans with a low affinity for methicillin, cephalothin, and cefamandole. | the presence of an additional penicillin-binding protein (pbp) was demonstrated in methicillin-resistant strains of staphylococcus epidermidis, s. haemolyticus, s. hominis, and s. simulans. in these four species, the apparent molecular mass of this protein was analogous to that of pbp 2' of methicillin-resistant s. aureus sr 1550-9. it exhibited a low affinity for methicillin, cephalothin, and cefamandole; and its synthesis was methicillin inducible. peptide mapping of this pbp from the four spe ... | 1990 | 2285281 |
| mathematical modeling of antimicrobial susceptibility data of staphylococcus haemolyticus for 11 antimicrobial agents, including three experimental glycopeptides and an experimental lipoglycopeptide. | antimicrobial mic data were obtained for 96 strains of staphylococcus haemolyticus and the following 11 antimicrobial agents: methicillin, gentamicin, rifampin, fusidic acid, ciprofloxacin, vancomycin, teicoplanin; three experimental glycopeptides, mdl 62,873, mdl 62,208, and mdl 62,224; and an experimental lipoglycopeptide, ramoplanin. resistance to methicillin and gentamicin was present in over 50% of the strains, although resistance to the other agents was present in less than 10%. it is show ... | 1990 | 2285289 |
| fulminant falciparum malaria. | a case of fulminant falciparum malaria with a 35% parasitaemia, shock and subcoma was treated successfully by using parenteral chemotherapy, exchange transfusion, dexamethasone, circulatory support and mechanical ventilation. pathophysiology and complications of falciparum malaria are discussed. the treatment of severe malaria should aim for a fast reduction in parasitaemia and toxic products. an exchange transfusion can be additive to parenteral chemotherapy. blocking the over-reacting cell-med ... | 1990 | 2286734 |
| selection for vancomycin resistance in clinical isolates of staphylococcus haemolyticus. | killing curves were used to characterize staphylococcus haemolyticus isolates previously reported to contain subpopulations showing increased resistance to vancomycin. results suggested that vancomycin and teicoplanin were ineffective at a concentration of 8 micrograms/ml and growth was seen between 24 and 48 h. conversely, the lipopeptide antibiotic daptomycin at the same concentration rapidly killed tested strains by 6 h. various staphylococcal strains were examined to determine if vancomycin ... | 1990 | 2295858 |
| reactivity of coagulase-negative staphylococci isolated from cow and goat milk with monoclonal antibodies to staphylococcus aureus capsular polysaccharide types 5 and 8. | monoclonal antibodies to staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. eighteen (15.5%) isolates were typable: 13 staphylococcus haemolyticus, 1 s. hyicus, 1 s. simulans, and 1 s. warneri from bovine origin and 2 s. lentus from caprine origin. type 5 was predominant, accounting for about 89% of typable isolates. reactivity with monoclonal antibod ... | 1990 | 2312680 |
| peripheral teflon catheters. potential source for bacterial contamination of orthopedic implants? | to detect low numbers of bacterial cells on teflon catheters removed from peripheral veins, 74 catheter tips were incubated in thioglycollate media for up to ten days. fifteen (20.3%) of the catheters were found to be culture-positive. propionibacterium acnes was isolated from seven, staphylococcus epidermidis from six, and staphylococcus haemolyticus and staphylococcus hominis from one each, all common skin organisms associated with infection of implants. an increase in documentation of bacteri ... | 1990 | 2323139 |
| staphylococcal enterotoxins direct and trigger ctl killing of autologous hla-dr+ mononuclear leukocytes and freshly prepared leukemia cells. | attempts have been made to induce cytolytic t cells to kill target cells that do not express the appropriate target molecules by crosslinking the t cells and the target cells in various ways. one successful strategy has been to use heteroconjugates or bispecific monoclonal antibodies reacting with t cell molecules with activating properties (e.g., mab directed to cd3/tcr) and target cell surface antigens. in this report we show that staphylococcal enterotoxins (se) direct human t lymphocytes to ... | 1990 | 2383900 |
| [empirical treatment of febrile episodes in granulocytopenic patients with a combination of piperacillin and ofloxacin. preliminary study]. | we evaluated the efficacy and toxicity of piperacillin-ofloxacin as an empiric treatment of fever in patients with neutropenia. 24 febrile episodes occurring in 21 patients (mean neutropenia: 204/mm3) were treated. the neutropenia was due to an hematologic malignancy in 6 cases and to chemotherapy in 15 cases. fever was related to septicemia in 4 cases, urinary tract infection in 1 case, other infectious sites without microbiological documentation in 11 cases, and was of unknown origin in 8 case ... | 1990 | 2385453 |
| haemophilus influenzae bartholinitis. | | 1990 | 2387300 |
| effects of retrograde flow on measured blood volume, disse space, intracellular water space and drug extraction in the perfused rat liver: characterization by the multiple indicator dilution technique. | previous rat liver perfusion studies had shown a preferential derecruitment of downstream (phenacetin o-de-ethylation) in relation to upstream (acetaminophen sulfation) hepatocyte activities with reductions in prograde (normal) flow, with substrates entering from the portal vein and exiting the hepatic vein. the reduction in metabolic activities was found associated with a reduction in accessible intracellular water space (pang et al., j. pharmacokinet. biopharm. 16: 595, 1988). in the present s ... | 1990 | 2395120 |
| keratinolytic activity of cutaneous and oral bacteria. | a test was developed to measure the keratinolytic activity of cutaneous and oral bacteria. keratin, labeled with fluorescein isothiocyanate, was used in a phosphate buffer (ph 7.2) with 1 mm dithiothreitol. the degradation of keratin was estimated by measuring the fluorescence of the degradation products in the supernatant of the reaction mixtures in a luminescence spectrometer. several oral and cutaneous bacteria were investigated: bacteroides gingivalis, bacteroides intermedius, treponema dent ... | 1987 | 2434427 |
| comparison of the acute phase response of cultured morris hepatoma 7777 cells and of rat hepatocytes. | isolated morris hepatoma cells (line 7777) or adult rat hepatocytes were cultured for 3 days and daily production of four plasma proteins was estimated in the cell media by rocket immunoelectrophoresis with monospecific antisera. addition of cytokines from rat peritoneal macrophages to cultured hepatocytes or hepatoma cells augmented accumulation in the medium of two positive acute phase proteins: fibrinogen (fib) and cysteine proteinase inhibitor (cpi). at the same time synthesis of alpha-fetop ... | 1987 | 2443154 |
| blast colonies containing hemopoietic progenitor cells can give rise to abelson virus (a-mulv)-transformed cell lines. | we have established an in vitro system with which to examine the ability of abelson murine leukemia virus (a-mulv) to infect early hemopoietic progenitor cells. blast cell colonies containing less than 100 cells were shown to contain up to 85% of cells with secondary hemopoietic colony-forming ability. infection of cells from these blast colonies resulted in generation of transformed mast cell lines when a feeder was provided. morphological examination of cells taken from infected cultures at va ... | 1988 | 2446900 |
| activation of the mas oncogene during transfection of monoblastic cell line dna. | using the tumorigenicity assay, we observed an activation of the mas oncogene during transfection of human acute myelocytic leukemia (ctv-2) dna. respective transfectants contained amplified mas sequences characterized by rearrangements in 5' and 3' noncoding regions that were transcribed into a 3.3 kb mas rna species. our data represent the second report on activation of the mas gene. | 1988 | 2453759 |
| [studies of the antigenic structure of trichophyton equinum]. | in the studies on antigenic structure of t. equinum double diffusion precipitation test in agar gel and immunoelectrophoresis were used. while preparing precipitation sera in rabbits it was manifested that the best results are achieved by immunization with live antigen. in the group of metabolic antigens and water extracts of the strains of t. equinum which were tested, 10-11 serologically+ active fractions were found whereas in protein component only l1 fraction. in comparative studies based on ... | 1989 | 2518619 |
| [staphylococcus haemolyticus. bacterial resistance to teicoplanin and associated morpho-structural aspects]. | to improve our understanding of the bases of teicoplanin resistance we studied a "one-step" mutant of s. haemolyticus from a morphological point of view. the results show that the organization of peptidoglycan is altered in the resistant s. haemolyticus strain. it is suggested that the alterations, including the spongier cell wall structure, may prevent the teicoplanin from reaching its target. | 1989 | 2533873 |
| susceptibility of gram positive cocci to teicoplanin. three year survey (1984-1986) at perugia university. | from 1984 to 1986, 1038 gram positive cocci (865 staphylococci and 173 enterococci) have been tested for sensitivity to teicoplanin by the agar diffusion method: only one strain (staphylococcus haemolyticus) resulted to have no zone of inhibition around the teicoplanin disk. all staphylococcus haemolyticus strains showed higher mic: 2.79 mcg/ml after 24h of incubation and 20.50 mcg/ml after 48h. the reduced teicoplanin sensitivity of staphylococcus haemolyticus was not detected by the agar diffu ... | 1989 | 2535245 |
| activities of daptomycin and teicoplanin against staphylococcus haemolyticus and staphylococcus epidermidis, including evaluation of susceptibility testing recommendations. | the in vitro activities of daptomycin, teicoplanin, and three other antimicrobial agents were determined against 105 strains of staphylococcus haemolyticus and 92 strains of staphylococcus epidermidis. the mics for 90% of strains tested (mic90s) of fusidic acid and rifampin were less than or equal to 0.25 microgram/ml. the mic90s of daptomycin and vancomycin were less than or equal to 4 micrograms/ml. teicoplanin had a comparable mic90 of less than or equal to 4 micrograms/ml for isolates of s. ... | 1989 | 2543286 |
| a b-type pdgf receptor lacking most of the intracellular domain escapes degradation after ligand binding. | the characteristics of the human b-type platelet-derived-growth-factor (pdgf) receptor expressed in chinese hamster ovary (cho) cells, were compared with those of a mutant receptor lacking all but 19 amino acids of the intracellular domain. the transfected wild-type receptor was synthesized as a 160-kda precursor that was processed to 190 kda. each cho cell expressed 30,000-100,000 receptors which bound pdgf-bb with a kd of about 0.5 nm. analysis of pdgf-ab binding yielded non-linear scatchard p ... | 1989 | 2546765 |
| efficacy of rifabutin in the treatment of aids-related complex. | we performed a phase 1-2 antiviral dose escalation trial of rifabutin, a rifamycin antibiotic with anti-hiv-1 activity in vitro. we followed 16 men with aids-related complex (arc) for a mean duration of 29 weeks; the maximum toxicity-limited dose of rifabutin was 2400 mg/day, which was achieved in two patients. there was some evidence of anti-hiv-1 activity in two patients, one of whom had an improvement in immune status, but 11 of the 16 patients showed a deterioration in either virologic or im ... | 1989 | 2576627 |
| interferon in experimental autoimmune encephalomyelitis (eae): effects of exogeneous interferon on the antigen-enhanced adoptive transfer of eae. | the transfer of experimental autoimmune encephalomyelitis (eae) can be enhanced by incubating lymph node cells (lnc) from eae rats with myelin basic protein (mbp). addition of rat fibroblast interferon (rfifn) to cultures of eae-lnc just before the addition of mbp, resulted in the inhibition of the adoptive transfer of eae. both development of clinical signs, such as weight loss and hind limb paralysis, and histopathological lesions, lymphocytic perivascular infiltrates of the central nervous sy ... | 1985 | 2579912 |
| nerve growth factor counteracts the neurophysiological and neurochemical effects of chronic sciatic nerve section. | the sciatic nerve was sectioned unilaterally in rats and nerve growth factor (ngf) applied locally to the nerve stump for the following 10-14 days using an indwelling osmotic pump. the aim of the experiment was to test whether ngf had any effect on the previously reported neurophysiological and neurochemical events that occur central to a peripheral nerve lesion. the method of application allowed the sciatic nerve on the other side to be used as a control. primary afferent depolarization fell, a ... | 1985 | 2581648 |
| high-performance liquid chromatographic determination of pirprofen and five of its metabolites in human plasma without hydrolysis and in human urine before and after chemical hydrolysis. | selective high-performance liquid chromatographic methods for the simultaneous determination of pirprofen and five of its metabolites either in plasma or in urine before and after chemical hydrolysis were developed. after addition of an internal standard and a buffer, the compounds were extracted from plasma using reversed-phase c18 bond-elut columns and from urine using pre-packed silica extrelut 1 columns, back-extraction into sodium hydroxide and acidification of the alkaline phase before inj ... | 1989 | 2613803 |
| bacterial contamination of intraocular lenses: the source of the bacteria. | in order to investigate potential sources for the bacterial contamination of intraocular lenses, specimens were taken for culture from a variety of sites. a swab from the lid margin, the operating room air, an intraocular lens which was allowed to rest on the eye, and the sodium hyaluronate were cultured during routine intercapsular lens implant surgery on 31 patients. positive cultures were obtained from six intraocular lenses. subtyping by three independent methods showed that isolates from fi ... | 1989 | 2630346 |
| characteristic of marrow culture and clinical relevance in leukemia with 8/21 translocation. | karyotypes and in vitro cultures of bone marrow cells from eleven patients with acute myelogenous leukemia m2 type were performed. 8/21 translocation was found in 5 patients. in all of them the cultures in vitro shared one characteristic, i.e., clusters were small and less in number. these patients could relatively easily obtain complete remission. the similarity of cfu-gm in patients with t (8; 21) may be another important biological phenotype. | 1989 | 2630651 |
| characteristic of marrow culture and clinical relevance in leukemia with 8/21 translocation. | karyotypes and in vitro cultures of bone marrow cells from eleven patients with acute myelogenous leukemia m2 type were performed. 8/21 translocation was found in 5 patients. in all of them the cultures in vitro shared one characteristic, i.e., clusters were small and less in number. these patients could relatively easily obtain complete remission. the similarity of cfu-gm in patients with t (8; 21) may be another important biological phenotype. | 1989 | 2630651 |
| solute and water transport across the pulmonary epithelium: a new chapter in lung physiology inaugurated by alfred jost. | early experiments by jost and policard demonstrated the presence of secretory activity in the lungs of the fetal rabbit. subsequent work has demonstrated a system of active ion transport across the pulmonary epithelium of the sheep fetus, in which uphill movement of chloride ions to the pulmonary lumen provides the main force for lung liquid secretion. in the last 10-20% of gestation a reabsorptive mechanism in response to beta-adrenergic stimulation is developed which is brought into play in th ... | 1989 | 2663091 |
| assay for sialidase using erythrocytes and peroxidase-labeled peanut lectin. | a rapid and sensitive sialidase assay method based on peroxidase-labeled peanut lectin (pna) binding to desialylated erythrocyte is described. formalinized sheep erythrocytes were used both as a stable substrate for sialidase and as a target for the lectin. in the case of sialidases from vibrio cholerae and arthrobacter ureafaciens, a linear relationship was observed between the amount of peroxidase-labeled pna bound to erythrocytes and the enzyme amount. binding of the lectin to sialidase-treat ... | 1989 | 2688936 |
| characterization of toxigenic vibrios isolated from the freshwater environment of hiroshima, japan. | the occurrence and characterization of toxigenic vibrios in surface water and sediment samples of the fresh water environment of the ohta river were studied. the membrane filter, pad preenrichment technique, followed by the placement of membranes onto thiosulfate citrate-bile salt-sucrose agar, was used for the enumeration of total vibrios. qualitative examination of pathogenic vibrios was also attempted. in addition, a survey was conducted to determine the incidence of clostridium botulinum in ... | 1989 | 2690736 |
| characterization of toxigenic vibrios isolated from the freshwater environment of hiroshima, japan. | the occurrence and characterization of toxigenic vibrios in surface water and sediment samples of the fresh water environment of the ohta river were studied. the membrane filter, pad preenrichment technique, followed by the placement of membranes onto thiosulfate citrate-bile salt-sucrose agar, was used for the enumeration of total vibrios. qualitative examination of pathogenic vibrios was also attempted. in addition, a survey was conducted to determine the incidence of clostridium botulinum in ... | 1989 | 2690736 |
| pemphigoid, pemphigus and desmoplakin as antigenic markers of differentiation in normal and tumorigenic mouse keratinocyte lines. | the expression of differentiation stages in a murine epidermal cell transformation model has been investigated as a basis for studies of chemically-induced differentiation. antibodies in sera of patients with the autoimmune diseases bullous pemphigoid and pemphigus vulgaris exhibit specific reactivity to antigenic determinants of basal and spinous cells, respectively, in sections of mouse and human epidermis. in addition, spinous cells in epidermis are reactive with a mouse monoclonal antibody t ... | 1989 | 2691098 |
| falciparum malaria--present day problems. an experience with 425 cases. | clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ... | 1989 | 2693436 |