| vascular angiotensin ii and atrial natriuretic peptide receptors in normal and growth-retarded human placentae. | receptors for angiotensin ii (aii) and atrial natriuretic peptide (anp) were characterized in a membrane fraction from resistance-type artery from human placentae. placentae from normal pregnancies and pregnancies complicated by intrauterine growth retardation (iugr) were studied. high- and low-affinity receptors for aii (dissociation equilibrium constant (kd) 1.7 and 15.7 nmol/l respectively) and anp (kd 0.2 and 55.5 nmol/l respectively) were identified; these parameters were unchanged in iugr, ... | 1990 | 2169524 |
| the essential region for assembly and particle formation in hepatitis b virus surface antigen produced in yeast cells. | the hepatitis b virus (hbv) genome carries a hbv surface antigen (hbsag) gene that can encode a polypeptide of 226 amino acids (aa). this gene can be expressed in the yeast, saccharomyces cerevisiae, and the products can be assembled into 22-nm particles indistinguishable from those recovered from a patient's serum. we constructed a set of deletion derivatives of the hbsag gene, and examined the particle-forming ability of the resulting polypeptides by expressing the gene in yeast. elimination o ... | 1990 | 2197186 |
| cloning, expression and characterization of the sau3ai restriction and modification genes in staphylococcus carnosus tm300. | the genes encoding the restriction enzyme (enase) and modification enzyme (mtase) of staphylococcus aureus 3a (recognition sequence 5'-gatc-3') have been cloned in staphylococcus carnosus tm300 using the vector pca44. clones carrying both genes were isolated from dna libraries prepared with mboi + bamhi. the dna region encoding m.sau3ai was subcloned on a 3.66-kb ecorv fragment in vector pt181mcs. plasmids purified from the clones were resistant to digestion with sau3ai, indicating that the sau3 ... | 1990 | 2227451 |
| [estimate of aztreonam (azt) hydrolyzing enzymes from clinically isolated azt-resistant gram-negative bacilli]. | the mics of aztreonam (azt) against 590 clinical isolates of gram-negative bacilli were determined. about 13.4% (79 strains) of the isolates were azt-resistant (mic; 12.5 micrograms/ml less than or equal to). the resistance pattern against various beta-lactams and the effects on mics of combination of clavulanic acid (cva) and azt against azt resistant strains suggested that azt was inactivated by either type of iv (k1), va (oxa1), or pse2 beta-lactamases. | 1990 | 2258651 |
| evidence of a novel staphylococcal mec-encoded element (mecr) controlling expression of penicillin-binding protein 2'. | a region was identified on the methicillin resistance determinant (mec) isolated from staphylococcus epidermidis and cloned into staphylococcus carnosus which was responsible for a novel downregulation of the expression of methicillin resistance. the presence of this region reduced the overall expression of methicillin resistance and the synthesis of the mec-encoded penicillin-binding protein 2' (pbp 2') in s. carnosus. this region was located by bal31 deletion mutagenesis upstream of the struct ... | 1990 | 2285282 |
| polyostotic hyperostosis associated with oviductal tumor in a cockatiel. | a 10-year-old female cockatiel in a thin and depressed condition and with a 1 x 2-cm soft abdominal swelling was hospitalized. radiography revealed a well-circumscribed mass containing punctate areas of mineralization, displacing the gizzard cranially in the caudodorsal portion of the abdomen. there was bilateral, symmetric, generalized, increased medullary opacity of the radius, ulna, carpometacarpus, femur, tibiotarsus, and tarsometatarsus. these findings were compatible with polyostotic hyper ... | 1990 | 2312392 |
| modulation of tartrate-resistant acid phosphatase expression by calcitriol in csf-induced macrophage colonies. | the osteoclast is thought to be a hemopoietically derived cell, but questions exist about which hemopoietic growth factors are responsible for proliferation of osteoclast precursors. experiments were thus performed to see if recombinant human colony-stimulating factors (csfs) influenced the expression of tartrate-resistant acid phosphatase (trap), an osteoclast marker enzyme, by monkey bone marrow colonies in vitro. in addition, the effect of 1,25-dihydroxyvitamin d3 (calcitriol) on csf-induced ... | 1990 | 2323362 |
| radioiodine in human thyroid glands and incidence of thyroid cancer in cumbria. | | 1989 | 2504410 |
| in vitro evidence for a neurite growth-promoting activity in trembler mouse serum. | basal lamina components, such as heparan sulfate proteoglycan (hspg) and laminin play an important role in neuritic outgrowth for cns and pns neurons in culture. the mutant mouse 'trembler' is characterized by hypomyelinization and production of an excess of basal lamina layers around schwann cells in peripheral nerves. in order to analyse whether or not the serum of the mutant animals contains neurite outgrowth-promoting factors, we cultured rat spinal cord neurons in the presence of trembler s ... | 1989 | 2526989 |
| molecular characterization and sequence of phosphatidylinositol-specific phospholipase c of bacillus thuringiensis. | the gene encoding monophosphatidylinositol inositol phosphohydrolase (pi-specific phospholipase c, pi-plc) of bacillus thuringiensis was cloned in staphylococcus carnosus tm300. the complete coding region comprises 987 base pairs corresponding to a precursor protein of 329 amino acids (molecular weight, 38,095). the nh2-terminal sequence of the purified enzyme from escherichia coli indicated that the mature pi-plc consists of 299 amino acid residues with a molecular weight of 34,586. polyacrylam ... | 1989 | 2548063 |
| in vivo effect of a beta-adrenergic agonist on activity of calcium-dependent proteinases, their specific inhibitor, and cathepsins b and h in skeletal muscle. | deae-sephacel and phenyl-sepharose chromatography were compared as methods for separating and quantitatively isolating calpain i, calpain ii, and calpastatin from lamb muscle extracts. deae-sephacel chromatography gave greater than 90% recovery of all three proteins, while phenyl-sepharose gave only 70, 66, and 48% of the deae recovery of calpain i, calpain ii, and calpastatin, respectively. additionally, deae-sephacel chromatography was shown to effectively separate calpastatin and calpain i. c ... | 1989 | 2573315 |
| molecular cloning of cdna sequences for rat alpha 2-macroglobulin and measurement of its transcription during experimental inflammation. | poly(a)+ rna enriched in alpha 2-macroglobulin (alpha 2m) mrna isolated from livers of rats 18 h after injection of turpentine was used for the synthesis of double-stranded cdna. the double-stranded cdna was inserted into the psti site of the plasmid pbr322 by oligo(dg)-oligo(dc)-tailing technique. clones containing sequences complementary to alpha 2m mrna were selected by differential colony hybridization using 32p-labeled poly(a)+ rna and [32p]cdna from livers of control and turpentine-treated ... | 1985 | 2581948 |
| purification and substrate specificity of staphylococcus hyicus lipase. | the staphylococcus hyicus lipase gene has been cloned and expressed in staphylococcus carnosus. from the latter organism the enzyme was secreted into the medium as a protein with an apparent molecular mass of 86 kda. this protein was purified, and the amino-terminal sequence showed that the primary gene product was indeed cleaved at the proposed signal peptide cleavage site. the protein was purified from large-scale preparations after tryptic digestion. this limited proteolysis reduced the molec ... | 1989 | 2611229 |
| cloning, sequencing and overexpression of the mannitol-specific enzyme-iii-encoding gene of staphylococcus carnosus. | the gene which encodes the mannitol-specific enzyme iii (eiiimtl) of the phosphoenolpyruvate-dependent phosphotransferase system of staphylococcus carnosus, has been cloned. genomic libraries of s. carnosus dna were constructed using the expression vector puc19 and eiiimtl-producing clones were identified using rabbit polyclonal antiserum. a 700-bp dde i fragment, containing the complete gene encoding eiiimtl, was sequenced by the dideoxy chain-termination technique. upstream from the orf for ei ... | 1989 | 2684783 |
| pemphigoid, pemphigus and desmoplakin as antigenic markers of differentiation in normal and tumorigenic mouse keratinocyte lines. | the expression of differentiation stages in a murine epidermal cell transformation model has been investigated as a basis for studies of chemically-induced differentiation. antibodies in sera of patients with the autoimmune diseases bullous pemphigoid and pemphigus vulgaris exhibit specific reactivity to antigenic determinants of basal and spinous cells, respectively, in sections of mouse and human epidermis. in addition, spinous cells in epidermis are reactive with a mouse monoclonal antibody t ... | 1989 | 2691098 |
| [determination of isepamicin in the eluates from the dried blood spots on filter paper for monitoring of blood levels in a guinea-pig]. | in general, collection of serial blood samples from small experimental animals is difficult in terms of sampling site and operation technique. to overcome these problems, a simple reproducible method has been improved by the use of filter papers. whole blood obtained by venipuncture from the ear vein of guinea-pig was spotted onto a filter paper. isepamicin in the dried blood spot was extracted with 0.5 m na2hpo4 buffer by incubation and determined by fluorescence polarization immunoassay. linea ... | 1989 | 2698418 |
| sepsis model with reproducible manifestations of multiple organ failure (mof) and disseminated intravascular coagulation (dic). | a new reproducible animal model of sepsis was established to investigate interrelationship between disseminated intravascular coagulation (dic) and multiple organ failure (mof). sepsis was induced by injecting fecal suspension into bile duct of rabbits, in which the amount of endotoxin in circulating blood was gradually increased and manifestations of sepsis were confirmed. during 9 hours' observation period, an occurrence of hypercoagulable state leading to dic was evidenced by the abnormal hem ... | 1989 | 2727956 |
| biosynthetic human growth hormone in burned patients: a pilot study. | a 15-day, open trial was conducted in 12 adult burned patients of whom six were randomly allocated to form a control group and six to receive biosynthetic human growth hormone (somatropin). all were fed enterally and treated according to a standard burn protocol. the mean age, burn size, energy and nitrogen intakes were well matched in each group. the resting energy expenditures (ree) were comparable in each group and the rees exceeded the calculated basal energy expenditures by a mean of 36.8 + ... | 1989 | 2736055 |
| the origin of the low-frequency microphonic in the first cochlear turn of guinea-pig. | low-frequency microphonic potentials (100 hz to 2000 hz) have been measured in the first turn of the guinea pig cochlea before and after a variety of manipulations of the cochlea. these included ablation of the apical turns, iontophoresis of streptomycin, dc current injection into the first turn, acoustic trauma and two-tone interference with pure tones. these manipulations indicate that the low-frequency microphonic measured in the first turn and at the round window is generated predominantly b ... | 1989 | 2737964 |
| purification and partial characterization of the rhizobium leguminosarum biovar viciae ca2+-dependent adhesin, which mediates the first step in attachment of cells of the family rhizobiaceae to plant root hair tips. | the ca2+-dependent adhesin which mediates the first step in attachment of bacteria of the family rhizobiaceae to plant root hair tips was isolated from the surface of rhizobium leguminosarum biovar viciae cells; its ability to inhibit attachment of r. leguminosarum to pea root hair tips was used as a bioassay. isolated adhesin was found to be able to inhibit attachment of both carbon-limited and manganese-limited r. leguminosarum cells. a multicolumn purification procedure was developed which re ... | 1989 | 2738027 |
| neuromuscular effects of three phospholipases a2 from the venom of the australian king brown snake pseudechis australis. | three single chain phospholipases a2 (pa-10a, pa-11 and pa-13) isolated from australian king brown snake (pseudechis australis) venom were tested for effects on neuromuscular transmission and muscle contractility on chick biventer cervicis and mouse diaphragm preparations. at 1 microgram/ml (about 85 nm) and higher, pa-10a and pa-11 reduced responses of both preparations to indirect stimulation in a concentration-dependent manner. responses to direct muscle stimulation were generally reduced mor ... | 1989 | 2749754 |
| perioral anesthesia disrupts maternal behavior during early lactation in long-evans rats. | the role of perioral somatosensory stimulation from pups in maternal behavior was investigated in lactating long-evans rats (rattus norvegicus) during early lactation by local anesthesia of the mystacial pads with lidocaine, which blocks conduction in the infraorbital nerve. it was concluded that (1) acute perioral anesthesia markedly inhibited pup retrieval when the subjects had no formal pretreatment retrieval experience. further, these dams displayed little tolerance to the lidocaine treatmen ... | 1989 | 2757582 |
| a preliminary trial of the programmable implantable medication system for insulin delivery. | we undertook a trial to determine whether an implanted insulin-delivery system, the programmable implantable medication system (pims), could be used to treat patients with insulin-dependent diabetes. pims is a pulsatile, programmable pump with a battery life expectancy of five years. the reservoir is refilled transcutaneously every two months with a surfactant-stabilized human insulin preparation containing 400 u of insulin per milliliter. eighteen patients received pims-delivered insulin for 4 ... | 1989 | 2761602 |
| cloning and expression of staphylococcus saprophyticus urease gene sequences in staphylococcus carnosus and contribution of the enzyme to virulence. | the urease gene of staphylococcus saprophyticus ccm883 was cloned and expressed in staphylococcus carnosus tm300. in vitro translation of the cloned dna sequences revealed six polypeptides (of 70, 47, 29, 27, 20, and 17 kilodaltons) that were associated with enzyme activity. introduction of the cloned genes into a urease-negative mutant of s. saprophyticus restored the virulence of this strain, confirming our previous suggestion (s. gatermann, j. john, and r. marre, infect. immun. 57:110-116, 19 ... | 1989 | 2777370 |
| measuring short-term rates of milk synthesis in breast-feeding mothers. | a moire contouring method was used to measure changes in the volume of the breast between infant feeds. a tungsten-halogen light source obliquely illuminated the breast through a moving moire grid to produce contour shadows on the breast. photographs of the moire topographs were analysed to obtain three-dimensional co-ordinates of transverse sections through the breast. the cross-sectional areas were calculated using the trapezoidal rule and volumes were calculated by multiplying these areas by ... | 1989 | 2798753 |
| pneumocystis carinii infection. | | 1989 | 2799113 |
| beta-adrenoceptor-mediated modulation of calcium ionophore activated polymorphonuclear leucocytes. | beta-adrenoceptor-mediated modulation of calcium-mediated stimulus-response coupling was studied using calcium ionophore (a23187) activation of polymorphonuclear leucocytes (pmnl). oxygen metabolite generation was measured with luminol- and lucigenin-dependent chemiluminescence in both whole blood and isolated pmnl. isoprenaline reduced pmnl response by 53% in a dose-dependent fashion. the effect was saturable, stereoselective, antagonized by propranolol and significant at isoprenaline concentra ... | 1986 | 2873859 |
| beta-adrenoceptor-mediated modulation of calcium ionophore activated polymorphonuclear leucocytes. | beta-adrenoceptor-mediated modulation of calcium-mediated stimulus-response coupling was studied using calcium ionophore (a23187) activation of polymorphonuclear leucocytes (pmnl). oxygen metabolite generation was measured with luminol- and lucigenin-dependent chemiluminescence in both whole blood and isolated pmnl. isoprenaline reduced pmnl response by 53% in a dose-dependent fashion. the effect was saturable, stereoselective, antagonized by propranolol and significant at isoprenaline concentra ... | 1986 | 2873859 |
| gene therapy in man. recommendations of european medical research councils. | 1. the purpose of gene therapy currently under consideration is the correction of genetic defects; attempts to enhance general human characteristics should not be contemplated. only somatic cell gene therapy, resulting in non-heritable changes to particular body tissues, should be contemplated. germline therapy, for introduction of heritable genetic modifications, is not acceptable. further technical improvements in the expression of transferred genes in somatic cells will be necessary before su ... | 1988 | 2897529 |
| localization of the mdx mutation within the mouse dystrophin gene. | we have mapped human and mouse x chromosome-specific genomic and cdna probes through an interspecies mus musculus/spretus pedigree which contains the mdx mutation. the positions of these markers relative to one another and to the mdx mutation were delineated. using probes corresponding to segments of the human duchenne muscular dystrophy (dmd) gene transcript, the position of a cross-hybridizing mouse equivalent gene (mdmd) was located. in more than 200 animals mapped, three were identified whic ... | 1988 | 2903046 |
| cloning and expression of methicillin resistance from staphylococcus epidermidis in staphylococcus carnosus. | a 6.2-kilobase chromosomal dna fragment from a methicillin-resistant staphylococcus epidermidis strain was cloned into staphylococcus carnosus by using staphylococcal plasmid pca44 as the vector. the recombinant plasmid obtained, pbbb21, conferred methicillin resistance on its host and was responsible for the synthesis of a low-affinity penicillin-binding protein (pbp), pbp 2'. pbp 2' determined by the s. epidermidis dna and expressed as a membrane-bound pbp in s. carnosus reacted with monoclona ... | 1988 | 2903715 |
| endogenous dopamine and dopaminergic agonists modulate synaptic excitation in neostriatum: intracellular studies from naive and catecholamine-depleted rats. | intracellular recordings from slices of rat neostriatum were utilized to study the effects of endogenous dopamine and of exogenous dopaminergic agonists on the excitatory synaptic potentials evoked by the local stimulation of the slice. d-amphetamine (0.1-5 microm), as well as dopamine, produced a dose-dependent decrease of the excitatory synaptic potentials. this effect was blocked by membrane hyperpolarization. the blockade of potassium channels by intracellular cesium or by extracellular 4-am ... | 1988 | 2904663 |
| spontaneous regression of pulmonary metastases from renal cell carcinoma. | one year after nephrectomy for renal cell carcinoma in a seventy-year-old man pulmonary nodules developed which proved to be metastases by needle biopsy. chest radiographs thirteen and eighteen months later revealed complete spontaneous regression of the nodules without therapy. | 1989 | 2916288 |
| maternal aging as the important etiological factor in human aneuploidy. | | 1985 | 2937399 |
| differential effects of recombinant human leukocyte interferons on cell surface antigen expression. | human leukocyte (alpha) interferon (ifn-alpha) is composed of a multigene family within which at least eight different species have been expressed in escherichia coli, isolated, and shown to exert a wide range of biological activities on different human target cells. in this study we utilized eight species of ifn-alpha (a, b, c, d, f, i, j, and k) and investigated their respective capabilities to alter the proliferation of a human breast carcinoma cell line (mcf-7). the antigens studied were all ... | 1986 | 2944575 |
| [bacteriological examination of dental plaque in institutionalized mentally retarded persons, particularly emphasizing the occurrence of mutans streptococci]. | | 1988 | 2978608 |
| complete nucleotide sequence of the lipase gene from staphylococcus hyicus cloned in staphylococcus carnosus. | the lipase gene from staphylococcus hyicus subsp. hyicus was cloned in staphylococcus carnosus and escherichia coli. in both host organisms the lipase gene is expressed and the enzyme is released to the medium. the cloned dna insert is 2.5 kb in length and dna sequencing has revealed the location of the gene, the ribosomal binding site and the presence of a typical signal sequence. the open reading frame comprises 1923 nucleotides and gives a preprotein of 641 amino acids with a predicted mr of ... | 1985 | 2994017 |
| regulation of the glucose phosphotransferase system in brochothrix thermosphacta by membrane energization. | uptake of 2-deoxyglucose, alpha-methylglucopyranoside, and glucose into intact cells of brochothrix thermosphacta (formerly microbacterium thermosphactum, atcc 11509) was stimulated by kcn or cccp. the glucose analogs were recovered almost totally as the sugar phosphates. membrane vesicles were isolated from protoplasts and shown to be right side out by freeze fracturing and by using atpase as a marker for the cytoplasmic membrane surface. uptake of glucose into vesicles was dependent on the pre ... | 1985 | 2995314 |
| studies on lipase directed export of escherichia coli beta-lactamase in staphylococcus carnosus. | the lipase (lip) gene of staphylococcus hyicus was used to study the expression of the escherichia coli beta-lactamase (bla) gene in s. carnosus. the bla gene, devoid of its promotor and most of the signal sequence, was fused to the lip structural gene at various positions. a set of 11 secretion vectors (pll beta 1 to pll beta 11) was isolated and analysed. all secretion vectors caused beta-lactamase production and activity in s. carnosus. however, the amount of hybrid proteins secreted was infl ... | 1986 | 3018441 |
| [gluconokinase characteristics of the brain tissue of white rats]. | | 1986 | 3020459 |
| [gluconokinase characteristics of the brain tissue of white rats]. | | 1986 | 3020459 |
| topoisomerase from ustilago maydis forms a covalent complex with single-stranded dna through a phosphodiester bond to tyrosine. | highly purified topoisomerase from ustilago breaks single-stranded dna, forming a complex with protein covalently bound to the dna. methods used to detect the complexes include a nitrocellulose filter assay, electrophoresis of the dna-protein complex in agarose gels containing alkali, and isolation of the complex after removal of all but a small oligonucleotide fragment bound to the protein. the linkage of the ustilago topoisomerase is to the 3' end of the broken strand of dna. the dna-protein c ... | 1986 | 3026452 |
| topoisomerase from ustilago maydis forms a covalent complex with single-stranded dna through a phosphodiester bond to tyrosine. | highly purified topoisomerase from ustilago breaks single-stranded dna, forming a complex with protein covalently bound to the dna. methods used to detect the complexes include a nitrocellulose filter assay, electrophoresis of the dna-protein complex in agarose gels containing alkali, and isolation of the complex after removal of all but a small oligonucleotide fragment bound to the protein. the linkage of the ustilago topoisomerase is to the 3' end of the broken strand of dna. the dna-protein c ... | 1986 | 3026452 |
| staphylococcal phosphoenolpyruvate-dependent phosphotransferase system: purification and characterization of the mannitol-specific enzyme iiimtl of staphylococcus aureus and staphylococcus carnosus and homology with the enzyme iimtl of escherichia coli. | enzyme iiimtl is part of the mannitol phosphotransferase system of staphylococcus aureus and staphylococcus carnosus and is phosphorylated by phosphoenolpyruvate in a reaction sequence requiring enzyme i (phosphoenolpyruvate-protein phosphotransferase) and the histidine-containing protein hpr. in this paper, we report the isolation of iiimtl from both s. aureus and s. carnosus and the characterization of the active center. after phosphorylation of iiimtl with [32p]pep, enzyme i, and hpr, the pho ... | 1988 | 3064811 |
| maternal-fetal distribution of morphine and alfentanil in near-term sheep and rhesus monkeys. | maternal-fetal distribution of the opiate agents morphine and alfentanil was studied in 3 near-term sheep and rhesus monkeys. blood samples were obtained from maternal and fetal arteries for radioimmunoassay after placement of catheters under halothane anesthesia. fetal-maternal ratios (f/m) of plasma morphine concentrations for sheep increased from 0.04 to 0.47 during the first hour after bolus iv injection of 200 micrograms/kg morphine. however, in monkeys, f/m values were considerably higher, ... | 1986 | 3081306 |
| the role of pulmonary cellular reactions in the resistance of vaccinated mice to schistosoma mansoni. | a histopathological and ultrastructural study was made of schistosomula and associated inflammatory reactions in the lungs of normal mice, and mice previously vaccinated with irradiated cercariae. in normal mice at day 7 post-infection all schistosomula were located in blood vessels. from day 11 onwards an increasing proportion of schistosomula were intra-alveolar (80% from day 20). no cellular reactions were evident around intravascular parasites in normal mice but at later sampling times large ... | 1986 | 3088537 |
| the influence of the pattern of inflammation and administration of steroids on class ii mhc antigen expression in renal transplants. | we have investigated the expression of class ii major histocompatibility complex (mhc) antigens in human renal allografts before, during, and after the first episode(s) of rejection, and correlated the antigen expression with the cytological pattern of inflammation as well as with the extent of steroid administration. the results confirm that the class ii mhc antigens are rapidly lost from a renal transplant after successful transplantation. downregulation of graft class ii antigenicity was obse ... | 1986 | 3094208 |
| induction of tumor growth inhibitory factor (tgif) in human mononuclear cells by ok-432, a streptococcal preparation. | a tumor growth inhibitory factor (tgif) was induced in the culture supernatant from mixed culture of human peripheral blood mononuclear cells (pbmc) and a streptococcal preparation, ok-432, in vitro. the activity generated in the supernatant increased in a time-dependent fashion and first appeared 6 h after the initiation of culture, reaching its maximum around 48 h. the tgif was cytostatic against seven of ten human tumor targets, but not against three murine tumor targets. tumor cell growth wa ... | 1988 | 3141056 |
| new aspects in the pharmacology of kindling implications for mechanism of of action in kindling. | kindling is a long-lasting, transsynaptic, pathway-specific plastic change in brain function. it has been proposed as a model of neural plasticity, learning and memory, as well as a model of epilepsy. to elucidate the action of substances characterized by their ability to improve learning and memory and to have an activatory, protective and function restoring effect on nerve cells in distress, the effects of nootropic drugs and of antioxidative acting substances on the development of kindling an ... | 1987 | 3147457 |
| induction of creatine kinase in immature rat uteri by zearalenone, an estrogenic mycotoxin. | intraperitoneal administration of alpha-zearalenone (zen) to female immature rats induced synthesis of a uterine protein which has been identified as creatine kinase. the induced enzyme was purified to homogeneity by chromatography on deae sephacel and hydroxyapatite ultrogel. guinea pig antiserum against estrogen-induced uterine, rat uterine creatine kinase crossreacted with the zen-induced enzyme, indicating that zen exhibits an early estrogenic response in a manner analogous to natural estrog ... | 1985 | 3161514 |
| blastocystis hominis: clinical and therapeutic aspects. | | 1988 | 3176148 |
| electrophysiological effects of melperone on isolated rabbit heart muscles. | 1. electrophysiological effects of melperone on isolated atrial and ventricular muscle preparations of the rabbit were studied by a conventional microelectrode technique. 2. melperone (3.3 microm) prolonged the action potential duration and effective refractory period of the atrial preparations without affecting the maximum rate of depolarization (vmax). these effects of melperone on action potential duration and effective refractory period were inhibited by a low potassium perfusate (2.7 mm). 3 ... | 1988 | 3207973 |
| studies on type ii progesterone receptor in mcf-7 cells. | these experiments demonstrate for the first time the existence of a type ii progesterone receptor (rpii) in mcf-7 human breast cancer cells. rpii was shown to have a lower affinity for tritiated progesterone ([3h]pg) (kd greater than or equal to 13 nm) than classical rp (kd less than or equal to 3 nm). rpii was detected by cytosolic, nuclear, and whole cell assays of mcf-7 cells. scatchard analysis of [3h]pg binding data revealed that classical rp but not rpii could be recompartmentalized from t ... | 1988 | 3254626 |
| the action of the protein kinase c inhibitor, staurosporine, on human platelets. evidence against a regulatory role for protein kinase c in the formation of inositol trisphosphate by thrombin. | the ability of several putative inhibitors of protein kinase c (pkc) to block dioctanoylglycerol (dc8)-induced phosphorylation of a 47 kda protein (a recognized substrate for pkc) in human platelets was investigated. staurosporine (1 microm) caused complete inhibition of phosphorylation, whereas the other reagents were either inactive (polymyxin b) or gave only partial inhibition (c-1, h-7, tamoxifen). staurosporine (1 microm) fully inhibited the phosphorylation of the 47 kda protein in platelet ... | 1988 | 3257691 |
| glucose, the key substrate in the microbiological changes occurring in meat and certain meat products. | the literature dealing with the role of glucose in the microbiological changes of meat and certain meat products is reviewed. discussion is centered on two aspects. first, glucose plays a part in the selection of the dominant spoilage organisms, pseudomonas fragi, ps. lundensis, and ps. fluorescens, on red meat stored aerobically under chill (2-7 degrees c) conditions. it is concluded that the pseudomonads flourish because they convert glucose to the less commonly used substrate, gluconate. the ... | 1988 | 3293613 |
| localization of cations by pyroantimonate. ii. electron probe microanalysis of calcium and sodium in skeletal muscle of mouse. | a new formulation of the pyroantimonate (pa) method for localization of calcium and sodium is proposed and evaluated in mouse skeletal muscle. this study, performed at the ultrastructural level by means of transmission electron microscopy (tem) and electron probe microanalysis (epma), completes a previous work done at the optical level with analytical ion microscopy (aim), which enabled us to define the appropriate composition of fixatives. in our present experiments, calcium and sodium were sho ... | 1988 | 3335770 |
| localization of cations by pyroantimonate. ii. electron probe microanalysis of calcium and sodium in skeletal muscle of mouse. | a new formulation of the pyroantimonate (pa) method for localization of calcium and sodium is proposed and evaluated in mouse skeletal muscle. this study, performed at the ultrastructural level by means of transmission electron microscopy (tem) and electron probe microanalysis (epma), completes a previous work done at the optical level with analytical ion microscopy (aim), which enabled us to define the appropriate composition of fixatives. in our present experiments, calcium and sodium were sho ... | 1988 | 3335770 |
| development of dopamine-containing neurons and dopamine uptake in embryos of hirudo medicinalis. | the embryonic development of neurons which contain or take up dopamine was studied with glyoxylic acid histofluorescence in hirudo medicinalis. beginning at the time of the formation of the tail ganglion, one pair of dopamine-containing neurons was stained per segmental ganglion. the normal outgrowth of the cell bodies into the anterior roots was prevented in isolated and cultured chains of embryonic ganglia. preincubation of intact embryos in dopamine led to the staining of additional neurons a ... | 1988 | 3396761 |
| ring-opened 7-methylguanine residues in dna are a block to in vitro dna synthesis. | single-stranded m13mp18 phage dna was methylated with dimethylsulfate (dms), and further treated with alkali to ring-open n7-methylguanine residues and yield 2-6-diamino-4-hydroxy-5n-methylformamidopyrimidine (fapy) residues. nucleotide incorporation during in vitro dna synthesis on methylated template using e. coli dna polymerase klenow fragment (kf polymerase) was reduced compared to the unmethylated template. additional treatment of the methylated template with naoh to generate fapy residues, ... | 1988 | 3399381 |
| experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination. | the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ... | 1988 | 3421507 |
| an automated perifusion system for the study of rat spermatogenesis in vitro. | we report details of an automated perifusion system for the study of spermatogenesis in vitro. a potential of this new system is the evaluation of hormone/growth factor effects on the mitotic clonal expansion of spermatogonia and the meiotic and postmeiotic clonal differentiation of spermatogenic cells in coculture with sertoli cells and in incubated seminiferous tubular segments. | 1987 | 3445968 |
| the necessary minimal duration of final long-term toxicologic tests of drugs. | the optimal, and thus mandatory duration of final, long-term toxicologic tests of drugs in animals prior to marketing for use in human beings remains controversial. some regulatory authorities contend that exposure for 6 or 12 months is adequate. however, the bureau of human prescription drugs of the health protection branch of health and welfare canada has evaluated a number of confidential reports from manufacturers in which significant, non-neoplastic, pathologic changes occurred only after e ... | 1986 | 3516779 |
| effects of roll tilt of the animal and neck rotation on different size vestibulospinal neurons in decerebrate cats with the cerebellum intact. | in decerebrate cats with the cerebellum intact we recorded the activity of lateral vestibulospinal neurons projecting to lumbosacral segments of the spinal cord (ivs neurons) and related the resting discharge, as well as the response characteristics of these neurons to roll tilt of the animal and neck rotation, with the cell size inferred from the conduction velocity of the corresponding axons. a slight negative correlation was found between resting discharge rate and conduction velocity of the ... | 1987 | 3615170 |
| effect of sterigmatocystin, a carcinogenic mycotoxin, on urea cycle enzymes and related metabolites in rat liver. | | 1987 | 3623596 |
| intrinsic circuitry involving the local axon collaterals of corticothalamic projection cells in mouse smi cortex. | the objective of this study was to identify the components involved in a local synaptic circuit in the mouse cerebral cortex. the local axon collaterals of corticothalamic (ct) projection cells in the posteromedial barrel subfield area of primary somatosensory cortex were labeled by the retrograde transport of horseradish peroxidase injected into the ipsilateral thalamus. thalamocortical (tc) axon terminals in the same region of cortex were labeled by lesion induced degeneration. ct axon termina ... | 1987 | 3624546 |
| effect of 2-deoxy-d-glucose on lipolytic processes in blood and adipose tissue of rat. | the effect of 2-deoxy-d-glucose on lipolytic processes in the blood and adipose tissue was studied. rats treated with this antimetabolite showed a significant increase in serum glucose, ffa and glycerol level, as well as in the lipid mobilizing activity. on the other hand, the lipolytic activity of rat serum decreased when compared to control group. from these results it may be concluded that during hypothermia induced by administration of 2-deoxy-d-glucose intracellular, but not intravascular, ... | 1985 | 3837983 |
| cloning and expression of the alpha-amylase gene from bacillus stearothermophilus in several staphylococcal species. | the plasmid-coded alpha-amylase gene of bacillus stearothermophilus (amy) was cloned in staphylococcus carnosus using plasmid pca43 as a vector. the amy gene was located on a 5.4-kb hindiii dna fragment of the hybrid plasmid pamy7. when transformed into other staphylococcal species, plasmid pamy7 exhibited marked differences in the production of alpha-amylase (alpha amy). most active for heterospecific alpha amy production was staphylococcus aureus. in its culture supernatant nearly half as much ... | 1985 | 3876968 |
| selectivity in sympathetic innervation during development and regeneration in the rat. | | 1985 | 3891400 |
| mica sandwich technique for preparing macromolecules for rotary shadowing. | the sandwich technique, in which a drop of sample solution is spread into a thin layer between two pieces of freshly cleaved mica, is a simple-to-use alternative to spraying for depositing macromolecules onto mica. test specimens of collagen molecules and actin filaments were found to suffer less shear-induced damage, they were more uniformly distributed, and only very small sample volumes were needed. either drying from a glycerol solution (40-70% v/v) or freeze-drying can be employed. glycerol ... | 1985 | 3903184 |
| quantitative and temporal analyses of murine antibody response in serum and gut secretions to infection with giardia muris. | we analyzed the appearance and level of giardia muris-specific antibody of immunoglobulin a (iga), igg, and igm isotypes, at weekly intervals, over the course of a 7-week infection in balb/c and c57bl/6 mice. using sensitive immunoradiometric assays, we observed that iga antibody was the only detectable anti-g. muris antibody in intestinal secretions throughout the course of infection. no secreted igg or igm anti-g. muris antibody was detected even in concentrated intestinal secretions. the expu ... | 1986 | 3957428 |
| capnocytophaga keratitis. | we report two cases of bacterial keratitis caused by capnocytophaga, a genus of capnophilic gram negative bacilli. both responded to topical and subconjunctival clindamycin. | 1985 | 3978064 |
| polyamines and putreanine relax respiratory tract smooth muscle in the guinea-pig. | spermidine, spermine, and putreanine, a metabolite of spermidine, caused transient relaxation of the isolated guinea-pig trachealis. the spermidine effect was not blocked by ouabain, propranolol or indomethacin. spermidine treatment was shown to desensitize the tissue to subsequent additions of spermidine. after exposure to spermidine and repeated washes with spermidine-free solution, trachealis responded to kcl (30 mm) with a paradoxical relaxation. this relaxation was not blocked by propranolo ... | 1985 | 4054214 |
| the application of the immunoenzyme method in microbiology. detection of anti-treponema and anti-toxoplasma antibodies. | | 1973 | 4121139 |
| studies on the location and orientation of proteins in the sarcoplasmic reticulum. | | 1973 | 4131254 |
| studies on the location and orientation of proteins in the sarcoplasmic reticulum. | | 1973 | 4131254 |
| pasteurella infection in irradiated hamsters. | | 1970 | 4247040 |
| [epizootiology of foot-and-mouth disease in kuban]. | | 1970 | 4249236 |
| etiology of congenital heart disease. | | 1970 | 4256571 |
| histochemical fibre types in the mammalian diaphragm. | | 1972 | 4263892 |
| an abdominal approach for producing permanent omasal fistulae in the ox and sheep. | | 1972 | 4266091 |
| possible role of brain serotonin and dopamine in controlling male sexual behavior. | | 1974 | 4276859 |
| enzymic activities of lipofuscin age pigments: comparative histochemical and biochemical studies. | | 1965 | 4284732 |
| [enzyme histochemical studies of blood vessel walls in experimental animals]. | | 1965 | 4284807 |
| cadmium-induced selective lesions of sensory ganglia. | | 1967 | 4290939 |
| cadmium-induced selective lesions of sensory ganglia. | | 1967 | 4290939 |
| effect of polynucleotides on aminoacyl transfer ribonucleic acid synthetases. 3. inhibition of glutamyl transfer ribonucleic acid synthetase by natural polynucleotides. | | 1968 | 4299520 |
| responsiveness of thalamic and cortical motor relays during arousal and various stages of sleep. | | 1969 | 4304625 |
| microbiological aspects of radiopasteurized chicken. nyo-3733-3. | | 1969 | 4313246 |
| coccidiosis with concurrent marek's disease. | | 1971 | 4325724 |
| coccidiosis with concurrent marek's disease. | | 1971 | 4325724 |
| response to sympathetic stimulation during angiotensin tachyphylaxis in dog's paw. | | 1972 | 4337941 |
| response to sympathetic stimulation during angiotensin tachyphylaxis in dog's paw. | | 1972 | 4337941 |
| isolation of clostridium absonum and its cultural and biochemical properties. | a new procedure for isolation of clostridium absonum was devised. sixtyseven strains of c. absonum were isolated from 135 soil samples, but no strain of c. absonum could be found from human fecal samples. the lecithinase, hemolysin, and lethal toxin in the culture filtrates of this species exhibited low avidity for c. perfringens type a antitoxin. the three activities were inseparable by the present method of purification. a reinvestigation of biochemical properties revealed that incomplete supp ... | 1974 | 4357934 |
| studies on the lysosomes of l132 cells infected with either rhinovirus type 2 or poliovirus type 1. | | 1974 | 4362408 |
| biochemical and ultrastructural changes in liver endoplasmic reticular fractions during q-fever. | | 1968 | 4384111 |
| increased enzyme activity in vitro and metabolism of substrates in vivo. | | 1969 | 4390428 |
| increased enzyme activity in vitro and metabolism of substrates in vivo. | | 1969 | 4390428 |
| local administration of orgotein in horses. | | 1974 | 4414502 |
| [structural analysis of the internal ear]. | | 1973 | 4540263 |
| effect of epinephrine-liberated pancreatic substance on uptake of alpha aminoisobutyric acid by isolated perfused rat liver. | | 1972 | 4662151 |