| a separation of staphylococci and micrococci based on serological reactivity with antiserum specific for polyglycerophosphate. | a serological reaction with the antiserum against heterophile polyglycerophosphate (pgp) was evaluated for genus level differentiation among strains of staphylococcus and micrococcus-sarcina spp.. hot saline extracts from whole cells of staphylococcus spp. strongly reacted with the pgp antiserum, whereas those of micrococcus-sarcina spp. did not. likewise, phenol-water extracts from whole cells of micrococcus-sarcina spp. were not reactive with the pgp antiserum, although the extracts of staphyl ... | 1978 | 97498 |
| microbial degradation of [c14c]polystyrene and 1,3-diphenylbutane. | microbial degradation of [beta-14c]polystyrene and 1,3-diphenylbutane, a compound structurally representing the smallest repeating unit of styrene (dimer), was investigated in soil and liquid enrichment cultures. degradation rates in soil, as determined by 14co2 evolution from applied [14c]polystyrene, varied from 1.5 to 3.0% for a 4-month period. although relatively low, these percentages were 15 to 30 times greater than values previously reported. enrichment cultures, containing 1,3-diphenylbu ... | 1978 | 98222 |
| new peroxides and their antimicrobial activity. | antimicrobial activity of hydroperoxides and cyclic peroxides has been studied. 2,4-dihydroperoxy-2,4-dimethylpentane (1) and 2,5-dihydroperoxy-2,5-dimethylhexane (2) were active against microorganism, but 1 showed a large specially against c. albicans, m. luteus and b. subtilis with a mic value of 31.5 mcg/ml. | 1978 | 98815 |
| formation of spirodilactone of 4-(2'-carboxyphenyl)-4,4-dihydroxybutyrate from 2-succinylbenzoate in cell-free extracts of micrococcus luteus. | the enzyme mediated atp- and, to a lesser extent, coash-dependent synthesis of the spirodilactone of 4-(2'-carboxyphenyl)-4,4-dihydroxybutyrate from 2-succinylbenzoate has been demonstrated in membrane-free extracts of micrococcus luteus and escherichia coli. the suggestion is made that the spirodilactone is the product of an aberrant reaction involving a compound that is normally an intermediate in the conversion of 2-succinylbenzoate to 1,4-dihydroxy-2-naphthoate. | 1978 | 99177 |
| addition of poly(adenylic acid) to rna using polynucleotide phosphorylase: an improved method for electron microscopic visualization of rna-dna hybrids. | we have observed that the enzyme polynucleotide phosphorylas from m. luteus or from e. coli will polymerize adenosine (a) from adenosine diphosphate onto 3' ends of rna molecules. for gene mapping, the poly(a)-tailed rna is hybridized to its complementary sequence on a longer dna strand. the position of the poly(a)tail, and thus the position of the 3' end of the rna on the dna strand, can then be observed by electron microscopy. our preferred mapping technique involves the synthesis of a poly(a) ... | 1978 | 100138 |
| [comparative study of the bactericidal action of the body of ornithodoros papillipes ticks]. | experiments in vivo have shown that the ticks of ornithodoros papillipes inhibit both gram-positive and gram-negative bacteria. on gram-positive microorganisms most sensitive to bactericidal effect of ticks have turned to be micrococci, staphylococci, streptococci and bacteria of diphtheria, of gram-negative ones--bacillus coli. of gram-positive microorganisms less sensitive were listeria, of gram-negative ones--salmonellae, agents of tularemia and pseudotuberculosis. at the infection with bacte ... | 1978 | 100756 |
| [examination of market carps for residues of antimicrobial agents]. | the residues of inhibitory substances in the muscle, hepatopancreas, bile, and pyloric caeca of 30 carps from ponds south of brno were examined by the microbiological diffusion method. the following testing micro-organisms were used: b. cereus var. mycoides (atcc 11778), b. subtilis (atcc 6633), sarcina lutea (atcc 9341), and micrococcus flavus (atcc 10240). residues of inhibitory substances were not found in any of the muscle samples tested. the rest of the tested tissues showed, in some cases, ... | 1978 | 100930 |
| sequence of a rabbit anti-micrococcus lysodeikticus antibody light chain. | the complete sequence of rabbit antibody light-chain l 120 has been elucidated. the antibody was raised against micrococcus lysodeikticus bacteria and is specific for the external part of the cell wall. all protein used in this work was obtained from a single 50-ml bleeding. the variable region of l 120 is compared to 13 other sequences of chains of different specificities. the constant region of this b4 k chain is identical to that of two other constant regions published earlier. the general st ... | 1978 | 101240 |
| [restriction of washing and its effect to the normal human skin flora. quantitative and qualitative investigations of the aerobic skin flora (author's transl)]. | the behaviour of the aerobic skin flora of the flexor sides of the forearms, under a three-week restriction of washing, was investigated in twenty-four patients for its quantitative and qualitative aspects. the combined scrabbing-washing method was used as described by burtenshaw with ringer's solution. after a three-week restriction of washing, using the wilcoxon test, statistically no significant changes in the total number of microorganisms were found in areas investigated in the intermittent ... | 1978 | 102263 |
| particle-based axenic media for tetrahymenids. | autoclavable, natural particulate media simplify axenic cultivation of tetrahymenid ciliates and presumably favor selection for phagotrophy. viability is at least 2 months at room temperature (24-26 c) for the lipid-sensitive tetrahymenids tetrahymena setosa, t. corlissi, t. paravorax, t. limacis, and t. patula, also for t. rostrata and (at 12 c), for strains of the t. pyriformis complex and glaucoma chattoni. a typical medium consists of crude soy "lecithin" + skim milk powder + saccharomyces c ... | 1978 | 102786 |
| quantitative studies on competitive activities of skin bacteria growing on solid media. | earlier quantitative investigations of antagonism between skin bacteria were based on the use of liquid cultures, but a more realistic model has now been devised, based on the use of the surfaces of solid media. pure or mixed inocula were spread evenly over suitable agar media in petri dishes marked out with a standard grid. growth curves were constructed from viable counts of the surface bacteria after they had been removed from excised squares of the agar media and dispersed. the method was hi ... | 1978 | 102799 |
| [microbiological studies on bacterial spectra of milk samples from healthy udder quarters and from those with increased cell counts and/or conductivity values]. | the germ levels of 2,182 milk samples obtained from udder quarters with subclinical mastitis were compared to milk sampled from 2,061 udder quarters with physiological cell counts or conductivity values. three cattle herds were involved in the test programme. no germ growth was established from 9.5 per cent of all samples taken from udder quarters with increased cell counts and conductivities and from 4.1 per cent of those samples taken from intact udder quarters. samples taken from udder quarte ... | 1978 | 104694 |
| comparative in vivo nitrogen-15 nuclear magnetic resonance study of the cell wall components of five gram-positive bacteria. | the proton-decoupled 9.12 mhz 15n nmr spectra of 15n-labeled bacillus subtilis, bacillus licheniformis, staphylococcus auresu, streptococcus faecalis, and micrococcus lysodeikticus intact cells, isolated cells walls, and cell wall digests have been examined. the general characteristics of gram-positive bacteria 15n nmr spectra and described and spectral assignments are provided, which allow in vivo 15n nmr to be applied to a wide range of problems in bacterial cell wall research. the qualitative ... | 1979 | 105756 |
| [results of measuring the modulus of elasticity of microorganism cell walls]. | the elasticity modulus of escherichia coli cell walls was found to change in the course of growth of the culture. it decreased in the middle of the exponential phase, apparently as a result of intensification of biosynthetic and related to them transport processes at this period. if the cells were treated with edta, their elasticity modulus was lower, by an order of magnitude, than that of the control cell walls. if the cells were treated with lysozyme, the elasticity modulus of their walls decr ... | 1979 | 106226 |
| interaction of phenosafranine with nucleic acids and model polyphosphates. iii. heterogeneity in phenosafranine interactions with dna base pairs. | fluorescence and circular dichroism spectral measurements, thermal denaturation studies and binding competition experiments with netropsin and actinomycin d were carried out in systems containing phenosafranine bound to dna's differing in base composition. the investigated properties exhibit a heterogeneity related to the content of a.t and g.c pairs in dna and to the nature of phenosafranine binding modes. at low level of saturation of binding sites (r less than 0.1) phenosafranine does not sho ... | 1979 | 106900 |
| [inorganic ion transport in bacteria]. | | 1979 | 107670 |
| preparation and antibacterial activity upon micrococcus luteus of derivatives of iturin a, mycosubtilin and bacillomycin l, antibiotics from bacillus subtilis. | methylated and acetylated derivatives of iturin a and mycosubtilin and methylated derivatives of bacillomycin l were prepared and their antibacterial activity on micrococcus luteus was compared with the activity of the original substance. the results obtained show the importance of polar groups for the antibiotic activity of the substances of iturin group. | 1979 | 108238 |
| interactions between bacterial membranes and peptidolipids: lysis of micrococcus luteus protoplasts by derivatives of peptidolipidic antibiotics from bacillus subtilis. | the lysis of protoplasts of micrococcus luteus has been tested with various derivatives of three peptidolipidic antibiotics: iturin a, mycosubtilin and bacillomycin l. the lytic activity is dependent to the nature of the substituting group and to the position of the substituted aminoacid residue. the acetylation of oh groups leads to a decrease of the lytic activity of the natural antibiotics. the methylation of aspartyl residues of bacillomycin l gives a strong lytic activity while natural baci ... | 1979 | 109121 |
| [daily bath and its effect on the normal human skin flora quantitative and qualitative investigations of the aerobic skin flora (author's transl)]. | the behaviour of the aerobic skin flora of the flexor sides of the forearm, under a daily repeated bath during 21 days, using two marketable bath-supplements, was investigated in 24 volunteers for its quantitative and qualitative aspects. the combined scrubbing-washing-method was used as described by burtenshaw with ringer's solution. after the daily baths, using wilcoxon-test, statistically no significant changes in the total number of microorganizations and in the number of the single groups o ... | 1979 | 111626 |
| induction of bacteriolytic enzyme from pyocinogenic pseudomonas aeruginosa and its enzymatic properties. | mitomycin c induced a pyocinogenic pseudomonas aeruginosa p15 to produce a bacteriolytic enzyme, pr1-lysozyme, together with pyocin r1. no significant accumulation of the enzyme was observed inside the induced cells. the enzyme was partially purified by acrinol treatment and amberlie cg-50 column chromatography. the mode of action of the enzyme on the host bacterial cells as well as on micrococcus lysodeikticus cells or peptidoglycan isolated from salmonella typhimurium, was compared with that o ... | 1978 | 117282 |
| [effect of microorganisms on survival of trichomonas vaginalis in physiological fluid]. | | 1979 | 117638 |
| [studies on release mechanism of bactericidins from blood platelets (author's transl)]. | | 1979 | 118274 |
| [phagocytic ability of trichomonas vaginalis donné]. | | 1979 | 118590 |
| magnetic susceptibility of microorganisms. | total magnetic susceptibility of 13 species and varieties of bacteria was investigated using the relative method of guy. it has been established that the index of magnetic susceptibility is a constant characteristic of bacteria. total magnetic susceptibility ranged from --0.3295.10(-6) in escherichia p678 to --0.4965.10(-6) in proteus. it has also been established that magnetic susceptibility changes during long-term passages of bacteria in fluctuating +/- 0.1 oe) magnetic field. this is suggest ... | 1979 | 118995 |
| bacteriology of rattlesnake venom and implications for therapy. | although the incidence of infection secondary to the bites of venomous snakes remains unknown, the routine use of prophylactic antimicrobial therapy is advocated. in this study, the venom from 15 rattlesnakes was cultured, and 58 aerobic and 28 anaerobic strains of bacteria were isolated. the most common species isolated were pseudomonas aeruginosa, proteus species, coagulase-negative staphylocci, and clostridium species. bacteroides fragilis was also recovered. when the fang sheaths of four add ... | 1979 | 119002 |
| distribution of membrane-bound monoamine oxidase in bacteria. | the distribution of membrane-bound monoamine oxidase in 30 strains of various bacteria was studied. monoamine oxidase was determined by using an ammonia-selective electrode; analyses were sensitive and easy to perform. the enzyme was found in some strains of the family enterobacteriaceae, such as klebsiella, enterobacter, escherichia, salmonella, serratia, and proteus. among strains of other families of bacteria tested, only pseudomonas aeruginosa ifo 3901, micrococcus luteus ifo 12708, and brev ... | 1979 | 120132 |
| cell wall-bridge maintaining three dimensional structure of cell packets formed by the localized suppression of cell separation of a micrococcus lysodeikticus (luteus) mutant. | cell packets of micrococcus lysodeikticus (luteus) mutant strain mt grown in medium supplemented with trypsin consisted of a tetrad as the unit structure. an interstice was observed between the unit-tetrads, and a three dimensional structure of cell packets was maintained by the cell wall-bridge along the rim of the cell packets which linked each unit-tetrad. this unique structure of strain mt cell packets seemed to occur when the cell separation was suppressed locally, i.e., when the cross wall ... | 1979 | 120485 |
| induction of autoanti-idiotypic antibodies and effects on the subsequent immune response. | | 1979 | 120495 |
| [evaluation of the effectiveness of disinfection procedures under hospital conditions]. | | 1979 | 120560 |
| [some peculiarities of the kinetics of dna structural transition after thermal jump registered by hyperchromic effect]. | structural transition of dna is characterized by complex optical kinetic curve. the kinetic version of felsenfeld's method is used for estimation of concentrations of "denatured" at- and gc-base pairs in dna molecules, and the rate constants of the accumulation of "denatured" at as well as gc pairs have been estimated. the possible interpretation of the kinetic data obtained may be done by assumption that the fast stage of the process reflects the accumulation of the "denatured" at pairs, which ... | 1979 | 121149 |
| [the bacterial degradation of steroids in urine]. | | 1975 | 123677 |
| micrococcus lysodeikticus atpase. purification by preparative gel electrophoresis and subunit structure studied by urea and sodium dodecylsulfate gel electrophoresis. | micrococcus lysodeikticus atpase was purified by preparative gel electrophoresis after its "shodk wash" release from the membrane. the method afforded the highest yield of pure protein in the minimum time as compared with former purification procedures. the pure protein had a specific activity of 7 mumol pi-min- minus 1-mg- minus 1 with incubation times not longer than 3 min, 345 000 mol. wt and was not stimulated by trypsin. by gel electrophoresis at alkaline ph (8.5) in 8 m urea or in sokiu ... | 1975 | 123783 |
| [proton transfer from water to octane, catalyzed by soluble bacterial atpase]. | | 1975 | 124244 |
| classification of the bacteriophages of gram-positive cocci: micrococcus, staphylococcus, and streptococcus. | micrococcus, staphylococcus, and streptococcus phages are classified by morphology, serological properties and physical and chemical parameters of the virion and its nucleic acid. fourteen species are defined; they include 75 tailed phages belonging to 6 morphological groups. type species are proposed. three species seem to have phylogenetic relationships with bacillus phages. | 1975 | 124850 |
| deoxyribonucleic acid strandedness. partial characterization of the antigenic regions binding antibodies in lupus erythematosus serum. | this study shows that tritiated thymidine labeled dna prepared from mammalian cells by the marmur technique is a pure preparation of nucleic acid that is composed essentially of two populations of molecules. one molecular population consists of primarily double-standed nucleic acid, while the other population is of double-stranded nucleic acid with significant single-stranded regions. the double-stranded dna with single-stranded regions can, depending upon the length of the single strand, behave ... | 1975 | 125288 |
| rheumatoid factor appearance in micrococcus lysodeikticus immunization and its interference with allotype specific reactions. | rabbits hyperimmunized with micrococcus lysodeikticus produce, in addition to anti-cell wall antibodies, considerable amounts of anti-immunoglobulins or rheumatoid factors which can interfere with immune reactions. in particular, in immunodiffusion, they can reveal non precipitating systems for which the immunodiffusion typing sera are usually not tested. in our hands, rabbit allotypes d12 and a8 give rise to visible immunodiffusion reactions in presence of rheumatoid factors. inhibition of this ... | 1975 | 126553 |
| use of limulus assay to compare the biological activity of peptidoglycan and endotoxin. | the gelation of a lysate prepared from the amebocytes of limulus polyphemus, the horseshoe crab, has been used to detect endotoxin-like material in clinical studies and in "in vitro" experiments. the investigation of blood samples from 54 hospitalized patients suspected of having endotoxemia, revealed a positive limulus test in 14. infections due to gram-positive organisms were not associated with positive assays. these results were in agreement with the observation that living gram-positive mic ... | 1975 | 126558 |
| interaction of peptidoglycans with anti-iggs and with complement. | this report describes the interaction of peptidoglycan (streptococcus group a, staphylococcus epidermidis and micrococcus lysodeikticus) with 2 serum mediator systems, namely with the anti-igg system and with complement. the observation that the majority of rabbits hyperimmunized with a-variant streptococcal vaccine produced anti-group carbohydrate antisera containing anti-iggs and antibodies directed to peptidoglycan suggested that the production of these 2 latter antibodies was related. this v ... | 1975 | 126565 |
| staphylococcal micrococcins. i. isolation of antibiotic-producing strains. | seven strains belonging to the family micrococcaceae and possessing strong antagonistic properties against staph. aureus and several other gram-positive bacteria, have been isolated. the strains were characterized and identified, phage typed, tested for antibiotic-sensitivity and antagonistic activities. two patients of inhibition were noted. | 1975 | 126688 |
| antigenic and enzymatic architecture of micrococcus lysodeikticus membranes established by crossed immunoelectrophoresis. | by crossed immunoelectrophoresis with membrane antiserum, 17 antigens have been detected in fractions from plasma membranes of m. lysodeikticus solubilized with triton x-100. absorption tests with protoplasts have demonstrated that eight of the antigens are expressed on the surface. of these antigens the major one has been identified as a succinylated mannan. five of the principal immunoprecipitates unaffected by absorption with protoplasts were shown by zymograms to possess the following enzymi ... | 1975 | 127177 |
| [genetic bases for the selection of microorganisms using analogs]. | the main mechanisms are considered for biosynthesis processes regulation by means of repression and inhibition with final products. on the basis of these mechanisms the state and prospects of the directed alteration of various stages in regulating the synthesis of primary and secondary metabolites, using breeding selection of mutants resistant to analogues are discussed. the main regularities are presented for using analogues of primary metabolites in the breeding strains with overproduction of ... | 1975 | 128859 |
| [adenosine triphosphatase from the membrane of micrococcus lysodeikticus]. | a preparation of atpase with a high specific activity was isolated from the membrane of m. lysodeikticus. the enzyme was studied using uv-spectroscopy and circular dichroism. the homogeneity of the protein preparation was shown by gel electrophoresis. the catalytic properties of the enzyme were studied using steady state kinetic methods. the values of km app. and kcat were determined to be 6-10(-4) and 6 mumoles/mg/min respectively. it is shown that adp is an effective inhibitor of the atpase re ... | 1975 | 129172 |
| [potentialization of antibiotics by lytic enzymes]. | few lytic enzymes, specially papaine and lysozyme, acting on the membrane and cell wall structures facilitate effects of bacitracine, streptomycine and other antibiotics. streptomycino resistant strains became sensibles to this antibiotic after contact with papaine and lysozyme. the results of tests in physiological suspensions concern only the lytic activity of enzymes. the results on nutrient medium concern together lytic, and antibiotic activities. | 1975 | 129213 |
| usefulness of phage typing for identification of staphylococcus epidermidis and micrococcus strains. | | 1975 | 130090 |
| membrane adenosine triphosphatase of micrococcus lysodeikticus. isolation of two forms of the enzyme complex and correlation between ezymatic stability, latency and activity. | two new forms of the plasma membrane atp-ase of micrococcus lysodeikticus nctc 2665 were isolated from a sub-strain of the microorganism by polyacrylamide gel electrophoresis. one of them had a mol.wt of 368,000 and a very low specific activity (0.80 mumol.min-1.mg protein-1) that could not be stimulated by trypsin. this form has been called b1 (strain b, inactive). if the elctrophoresis was carried out in the presence of reducing agents (i.e., dithiothreitol) and the ph of the effluent maintain ... | 1976 | 130538 |
| me2+-(13 s) atpase from micrococcus sp. atcc 398e. the effect of trypsin on the purified enzyme. | by trypsin treatment of highly purified atpase (ec 3.6.1.3) from micrococcus sp. atcc 398e, two enzyme modifications have been obtained. (i) atpase ta, which has about the same activity as untreated atpase. (ii) a protein complex ti, which lacks atpase activity, but nevertheless binds atp as shown by affinity chromatography. trypsin primarily shortens the alpha-chains of the "native" enzyme to alpha-chains and removes the gamma-subunit, thus yielding atpase ta. the formation of the protein compl ... | 1976 | 131581 |
| micrococcus lysodeikticus membrane atpase. effect of trypsin on stimulation of a purified form of the enzyme and idenfification of its natural inhibitor. | a soluble purified form of micrococcus lysodeikticus atpase (form bat, from strain b, active, trypsin-stimulated) was stimulated 100% by trypsin and this stimulation was inhibited by preincubation of the protease with phenyl methyl sulphonylfluoride. this form of the enzyme was also stimulated 125-150% by filtration on sephadex g-200. analysis by sodium dodecyl sulphate-gel electrophoresis showed that stimulation of this form of m. lysodeikticus atpase was always accompanied by the disappearance ... | 1976 | 132193 |
| activation parameters of the adenosine triphosphatase of micrococcus lysodeikticus. a comparison of the soluble and membrane-bound forms of the enzyme. | the arrhenius plots for the membrane-bound atpase and its soluble form purified from micrococcus lysodeikticus, presented discontinuities near 30 degrees c at ph 7.5. glycerol-containing lipids were not responsible for these discontinuities. the values of the enthalpies of activation were 12 (soluble) and 22 (membrane-bound) kcal/mol (50.2 and 92.0 kj/mol) above 30 degrees c and 42 (soluble) and 29 (membrane-bound) kcal/mol (175.7 and 121.3 kj/mol) below that temperature. the results suggested t ... | 1976 | 133801 |
| response of membrane-bound atpase of micrococcus luteus to heat and ultraviolet light. | it is shown that the properties of atpase (ec 3.6.1.3) of micrococcus luteus depend only to some extent on the state of the membrane to which it is attached. its interaction with the membrane appears to be largely controlled by polar forces. it is shown, however, that the uv-sensitivity of the membrane-bound atpase is also significantly influenced by the state of membrane lipids. | 1976 | 134390 |
| membrane-bound f1 atpase from micrococcus sp. atcc 398e. purificationa and characterization ny affinity chromatography. | a chemically reactive atp analogue, 6-[(3-carboxy-4-nitrophenyl)thio]-9-beta-d-ribofuranosylpurine 5'-triphosphate (nbs6itp) has been synthesized. it has the ability to form stable thioether bonds between the 6-position of the purine ring and aliphatic mercapto groups. the nucleotide moiety of the reagent has been covalently bound to agarose, via iminobispropylamine and n-acetyl-homocysteine as space with the purpose of producing an affinity chromatography material. the affinity matrix binds sol ... | 1976 | 134891 |
| [ultrastructure and microflora in follicles and comedones]. | the resident flora of skin surface, sebaceous follicles, and acne comedones: pityrosporum, micrococci, and corynebacterium (propionibacterium) acnes is described. the nomenclature, topography, incidence, pathogenic role, and especially the ultrastructural morphology of these organisms are presented. | 1976 | 136430 |
| [mechanism of action of the specific inhibitor of respiration and phosphorylation in mitochondria--n-(n,n-di-2-chlorethyl)aminophenylacetic acid]. | an electrophilous inhibitor, p-(n,n-di-2-chloroethyl)amino-phenylacetic acid (i), specifically disturbs the mechanism of respiration and phosphorylation coupling in mitochondria. i inhibits respiration and atpase activity in intact mitochondria and does not affect these processes in mitochondria and submitochondrial particles with partially or completely impaired coupling system. the data obtained show that i inhibits protonophoric function of nadh-ferricianide reductase from submitochondrial pa ... | 1976 | 136999 |
| [effect of the dielectric permeability of the non-aqueous phase on enzymatic charge transfer through an oil-water inferface]. | charge transfer across the interface between two immiscible liquids in functioning enzyme membrane systems and chlorophyll is studied. phenomenological theory is developed describing the influence of the dielectric permeability of oil phase on the change of the potential difference at the interface during the charge transfer across oil/water. it is shown that change of the potential difference when varying dielectric permeability can be explained as a change of the activation energy of charge tr ... | 1976 | 137752 |
| the effect of atebrin on bacterial membrane adenosine triphosphatases in relation to the divalent cation used as substrate and/or activator. | the action of atebrin on purified adenosine triphosphatase (atpase) from micrococcus lysodeikticus was studied as well as on the membrane-bound and soluble atpases from escherichia coli and bacillus megaterium. atebrin inhibited the ca(2+)-dependent activity of all these enzymes, and the inhibition was reversed by an excess of ca(2+) ions. kinetic studies carried out with the purified enzyme from m. lysodeikticus showed that the inhibition by atebrin was strongly cooperative, suggesting the comp ... | 1977 | 138384 |
| optical properties and denaturation by guanidinium chloride and urea of the adenosine triphosphatase of micrococcus lysodeikticus. a comparison of four molecular forms of the enzyme. | 1. the fluorescence and circular dichroism of four homogeneous preparations of atpase (adenosine triphosphatase) from micrococcus lysodeikticus differing in molecular structure and enzymic properties were examined at ph 7.5 and 25 degrees. emission was maximum at 325 and 335 nm and the relative intensities at these wavelengths may be used to characterize the different atpase preparations. the circular-dichroism spectra exhibited negative extrema at 208 and 220 nm, and the relative value of the m ... | 1977 | 139887 |
| differential sensitivity to trypsin digestion of purified forms of micrococcus lysodeikticus atpase (bfi). a study of their structural and conformational differences and mechanism of conversion. | | 1977 | 141947 |
| [non-ionogenic detergents with antibacterial activity and their effect on bacterial membrane systems]. | certain new nonionogenic detergents which are hydroxy-polyethoxy-derivatives of dodecane show in vitro bacteriostatic action as related to gram-positive microorganisms. in this respect they are not inferior to chloride cetylpyridinium or sodium dodecyl sulphate. they are similar to chloride cetylpyridinium in their ability to disrupt the osmotic barrier of protoplasts and to inhibit nad-h-oxidase of membrane fractions of micrococcus lysodeikticus. unlike chloride cetylpyridinium and sodium dodec ... | 1977 | 142981 |
| activation parameters and molecular changes induced by substrate hydrolysis of the adenosine triphosphatase of micrococcus lysodeikticus. a comparison of three different soluble forms of the enzyme. | the arrhenius plots for the active and low activity soluble forms of the atpase purified from the membranes of micrococcus lysodeikticus grown at 30 degrees c presented discontinuities at 30 and 33 degrees c, respectively. their activation parameters differed, being highest for the low activity form of the enzyme. both forms underwent changes in their molecular properties as a consequence of being enzymically active, i.e., upon incubation with substrates at an adequate temperature. these changes ... | 1977 | 143600 |
| membrane asymmetry and expression of cell surface antigens of micrococcus lysodeikticus established by crossed immunoelectrophoresis. | crossed immunoelectrophoresis of triton x-100-solubilized plasma membranes of micrococcus lysodeikticus established the presence of 27 discrete antigens. individual antigens were identified as membrane components possessing enzyme activity by zymogram staining procedures and by reactivity of certain antigens with a selection of four lectins in the crossed-immunoelectrophoresis (immunoaffinoelectrophoresis) system. absorption experiments with intact, stable protoplasts and isolated membranes esta ... | 1977 | 144722 |
| f1-atpase from micrococcus sp. atcc 398. purification by ion-exchange chromatography and further characterization. (auto)proteolysis and dissociative effects. | the preparation of highly purified f1-atpase from micrococcus sp. atcc 398 by application of deae-sepharose cl-6b chromatography as final step is described. this enzyme consists of five subunits of different molecular weight: alpha (65000), beta (55000),gamma (35000), delta (20000), and epsilon (17000). disc electrophoresis on 5% polyacrylamide gels removes the epsilon-polypeptide yielding an active atpase complex with four different subunits: alpha, beta, gamma, delta. additionally, by variatio ... | 1977 | 145365 |
| [osmotic activity and ionic permeability of membrane vesicles from streptococcus faecalis and micrococcus lysodeikticus cells]. | membrane fractions containing osmotically active vesicles with sufficiently low membrane permeability for k+, na+ and cl- ions typical for the intact cell membrane were isolated from the cells of the glycolyzing bacterium streptococcus faecalis. in their osmotic properties and ionic permeability the membrane fractions of s. faecalis were found similar to those of the respiring bacterium micrococcus lysodeikticus, which are capable of the energy-dependent potassium transport. it may be thus assum ... | 1977 | 145881 |
| glycoprotein nature of energy-transducing atpases. chemical characterization of glycopeptides isolated from bacterial and chloroplast coupling factors. | | 1978 | 145961 |
| [microbiological studies on mouth-ringing, cooling and turbine-spray waters]. | microbiological examinations of mouth-cleaning, cooling, and turbine spray waters from various types of dental units showed different degrees of water contamination. the detection of pseudomonas aeruginosa, because of the classification of this organism as a "problem germ", indicates the necessity for a more critical analysis of water used in stomatology including the elimination of possible sources of contamination. | 1977 | 146368 |
| the functional tryptophan and tyrosine residues of f1 atpase of micrococcus sp. atcc 398. | | 1978 | 146676 |
| thermal denaturation of micrococcus lysodeikticus adenosine triphosphatase. influence of temperature on the circular dichroism, fluroescence and enzymic activity of the protein. | the soluble atpase (adenosine triphosphatase) from micrococcus lysodeikticus underwent a major unfolding transition when solutions of the enzyme at ph 7.5 were heated. the midpoint occurred at 46 degrees c when monitored by changes in enzymic activity and intrinsic fluorescence, and at 49 degrees c when monitored by circular dichroism. the products of thermal denaturation retained much secondary structure, and no evidence of subunit dissociation was detected after cooling at 20 degrees c. the th ... | 1978 | 147080 |
| characterization and affinity labeling of nucleotide binding sites of bacterial plasma membrane adenosine triphosphatase (f1). | | 1978 | 147101 |
| kinetic studies on bacterial plasma membrane atpase (f1). nucleotide-induced long term inactivation of atp hydrolyzing activity is linked to the formation of multiple "tight" enzyme nucleotide complexes. | adp and the atp analogs nb-s6itp (6-[(3-carboxy-4-nitrophenyl)thio]-9-beta-d-ribofuranosylpurine 5'-triphosphate) and amp-p(nh)p (adenyl-5'-yl imidodiphosphate) interact with soluble plasma membrane atpase (f1) from micrococcus species in two ways: (i) at short incubation times, these inhibitors exhibit the kinetics of competitive inhibition, (ii) at long incubation times, these inhibitors induce an inactivation of the atpase which can be reversed only in the case of amp-p(nh)p. kinetic treatmen ... | 1978 | 149127 |
| 8-azido-adenosine 5'-triphosphate as a photoaffinity label for bacterial f1 atpase. | 1. 8-azido-adenosine 5'-triphosphate (n83atp) is a suitable photoaffinity label for f1 atpase from micrococcus luteus. the nucleotide is a substrate in the presence of bivalent cations and inhibits the enzyme irreversibly upon irradiation with ultraviolet light above 300 nm. 2. more than 80% of the label is covalently bound to the beta subunits in the presence of bivalent cations. labeling and inactivation is decreased by protection with adp, atp or adenyl-5'-yl imidodiphosphate. to a much small ... | 1978 | 149660 |
| activity spectrum of micrococcal and staphylococcal phages. | the activity spectrum of 4 polyvalent staph. aureus-phages, of 22 phages from coagulase negative staphylococci and of 64 micrococcal phages was established on 20 staph. aureus-strains, 116 coagulase-negative staphylococci and 142 micrococci. staphylococcal phages showed to be only active on strains of the genus staphylococcus and on cocci related to this genus. micrococcal phages on the other hand lysed only micrococci. | 1978 | 151454 |
| the phage adsorption test: a simple method for differentiation between staphylococci and micrococci. | a simple phage adsorption test is recommended for differentiation between staphylococci and micrococci. all 132 strains of staphylococcus tested inactivated phage u16 significantly, whereas of 123 strains of micrococcus tested, only three strains of micrococcus candicans were able to adsorb this staphylococcal phage. there are special problems in the taxonomy of m. candicans. | 1978 | 151718 |
| a simple method of activity staining for phosphate-releasing enzymes in electrophoresis gels. | | 1978 | 152732 |
| [protein synthesis with cell extracts of micrococcus radiodurans (author's transl)]. | pure active ribosomes of cells of micrococcus radiodurans could be obtained when cultivated in trypton, glucose and nutrient broth by adding natrium citrate. the optimal conditions for a cell-free protein synthesis were investigated at the (polyuridylic acid) dependent polyphenylalanine synthesis. when exchanging ribosomes and s100-fractions with the corresponding fractions of e. coli, we found that the enzyme fractions of m. radiodurans extremely inhibit the ribosomal activity. the incorporatio ... | 1978 | 154232 |
| molecular properties of random coil and refolded forms of alpha and beta subunits of an energy transducing atpase from bacterial membranes. | | 1979 | 155472 |
| solubility characteristics of micrococcus lysodeikticus membrane components in detergents and chaotropic salts analyzed by immunoelectrophoresis. | in order to evalute the effectiveness and selectivity of various reagents in the solubilization of bacterial membranes, membranes of micrococcus lysodeikticus were treated with detergents and chaotropic agents. the composition of the extracts so obtained was analyzed by rocket and two-dimensional immunoelectrophoretic techniques. recoveery of succinate-, malate-, and reduced nicotinamide adenine dinucleotide- (nadh) dehydrogenases, atpase, succinylated lipomannan and cytochromes in the extracts ... | 1979 | 156556 |
| preparation of the soluble atpase from mitochondria, chloroplasts, and bacteria by the chloroform technique. | | 1979 | 156841 |
| purification and properties of the latent f1-aptase of micrococcus lysodeikticus. | the latent coupling factor (f1)-atpase of micrococcus lysodeikticus has been purified to homogeneity as determined by a number of criteria including, nondenaturing polyacrylamide gel electrophoresis, crossed immunoelectrophoresis and analytical ultracentrifugation. by inclusion of 1 mm phenylmethyl sulfonyl fluoride, a serine protease inhibitor, in the shock-wash step of release of f1 from the membranes, the spontaneous activation of both crude and purified atpase by endogenous membrane protease ... | 1979 | 157161 |
| [gamma-irradiated ribosomes from micrococcus radiodurans in a cell-free protein synthesizing system (author's transl)]. | gamma-irradiation inactivation of isolated ribosomes of micrococcus radiodurans was studied by examining poly u directed synthesis of polyphenylalanine. ribosomes of m. radiodurans did not show significant gamma-radiation sensitivity up to a dose of approx. 11.6 k gy. cells of m. radiodurans take up more magnesium than e. coli cells unter the same conditions. the magnesium content of ribosomes of m. radiodurans was 18% higher than that of e. coli ribosomes. a possible relation between mg2+-conte ... | 1979 | 158899 |
| immunochemical analysis of triton x-100-insoluble residues from micrococcus lysodeikticus membranes. | triton x-100-insoluble residues from micrococcus lysodeikticus membranes were analyzed by crossed immunoelectrophoresis after dispersal of the residues in sodium dodecyl sulfate (sds). conditions which produce no obvious distortion of the immunoprecipitate profile and which allow qualitative and quantitative analyses of the antigens present in the extracts are described. two main antigens were detected; these were identified as succinate dehydrogenase (ec 1.3.99.1) and adenosine triphosphatase ( ... | 1979 | 160415 |
| deoxyribonucleic acid polymerase iii of escherichia coli. characterization of associated exonuclease activities. | purified dna polymerase iii has two distinct exonuclease activities: one initiates hydrolsis at the 3 termini, and the other at the 5 termini of single-stranded dna. both exonucleases have the same relative mobility on polyacrylamide gels as the polymerase activity. molecular identity of the three activities is further indicated by their comparative rates of thermal inactivation and their sensitivity to ionic strength. the 3-5 exonuclease activity hydrolyzes only single-standed dna. the rate of ... | 1975 | 163228 |
| a comparison of the effects of daunomycin and adriamycin on various dna polymerases. | the effects of the anthracycline antiboties, daunomycin and adriamycin, on the dna-directed activities of dna polymerases from murine sarcoma virus, rat liver (high-molecular-weight species), escherichia coli, and micrococcus luteus were determined. under all conditions tested, these compounds had greater inhibitory effect against the viral polymerase than against cellular polymerase. the inhibition of murine sarcoma virus dna polymerase by daunomycin was competitive with respect to dna. for vir ... | 1975 | 163690 |
| induction of nutritional mutants of micrococcus glutamicus and their amino acid accumulation. | micrococcus glutamicus atcc 13032, a glutamic acid-producing organism, was treated with 0.2m ethylmethane sulfonate, the auxotrophs isolated showing varied patterns of extracellular amino acids. eighty auxotrophic strains were obtained, out of which 31 excreted 1.0-4.0 mg threonine per ml and all the auxotrophs required biotin for growth and production of the amino acid. eleven auxotrophs produced 1.5 to 3.0 mg alanine per ml and these auxotrophs required amino acids for their growth. other auxo ... | 1975 | 163783 |
| interaction of 4,5-dibromo-2,7-di-(acetatomercuri)-fluorescein with dnas of different base composition. | the changes in absorption spectra in the visible region observed on adding different naturally occurring and synthetic dna duplexes to solutions of 4,5-dibromo-2,7-di-(acetatomercuri)-fluorescein indicate that the mercurial reacts with polynucleotides of this type. the reaction is reversible as proved by adding excess of kcn which restores the original spectra of the free dye. the interaction is characterised also by quenching of the fluorescence of the dye and the induction of optical activity ... | 1975 | 164231 |
| the compatibility of netropsin and actinomycin binding to natural deoxyribonucleic acid. | the simultaneous binding of netropsin and actinomycin to four natural dnas was studied to determine the influence of one ligand on the binding of the other. actinomycin binds specifically to gc sites, whereas netropsin binds specifically to at sites. spectral titrations, thermal denaturation, and analytical buoyant density centrifugation were employed to measure the binding interference of these drugs. the binding of actinomycin to dna was decreased by the presence of netropsin. increasing the g ... | 1975 | 164447 |
| studies on interaction between histone v (f2c) and deoxyribonucleic acids. | histone v (2fc) from chick erythroctes was used in the study of its interaction with dna from various sources. complexes between this histone and dna were formed using the procedure of continuous nacl gradient dialysis in urea. two physical methods, namely thermal denaturation and circular dichroism (cd), were used as analytical tools. thermal denaturation of nucleohistone v with chick or calf thymus dna shows three melting bands: band i at 45-50 degrees corresponds to free base pairs; band ii a ... | 1975 | 164887 |
| interaction of clostridium perfringens theta-haemolysin, a contaminant of commercial phospholipase c, with erythrocyte ghost membranes and lipid dispersions. a morphological study. | commercially available preparations of phospholipase c from clostridium perfringens are commonly contaminated with theta haemolysin, one of a group of bacterial haemolysins called oxygen labile (o-labile) haemolysins. treatment of erythrocyte ghosts and a mixed lipid dispersion containing cholesterol with commercially available phospholipase c in the absence of ca-2+ and the presence of phosphate buffer and/or edta resulted in the formation and release of ring or arc-shaped structures. highly pu ... | 1975 | 164911 |
| determination of the 3' terminal nucleotide of dna fragments. | a new method for determining the 3'-terminal nucleotide of a dna strand is presented. use is made of the fact that one (and only one) 2',3'-dideoxyribonucleotide can be added to the 3'-end of a dna fragment with calf thymus terminal transferase. addition of more than one nucleotide analog per strand is impossible due to the absence of a 3'-terminal hydroxyl group. if the terminatind dideoxyribonucleotide contains an (alpha 32p) label, the resulting 3'-blocked strand can be digested by "nearest n ... | 1975 | 165468 |
| polynucleotide duplexes based on poly(7-deazaadenylic acid). | in order to find a poly(a)-poly(u) analog which could not form a triple-stranded complex and which would have a sufficiently high thermal stability to survive under physiological conditions, the interaction of poly(7-deazaadenylic acid) (poly(c-7a)) with modified polyuridylic acids was examined. mixing curves constructed by the method of continuous variation, isosbestic points and thermal melting profiles proved that poly(c-7a) formed only 1:1 complex with polyribothymidylic acid and poly(5-brom ... | 1975 | 166696 |
| dna sequence analysis. terminal sequences of bacteriophage phi80. | sequences of the cohesive ends and the 3'-terminal regions of phi80 dna have been determined. sequences of the cohesive ends were obtained through the use of two standard methods. the first method involved the incorporation of all four labeled deoxyribonucleotides into the phi80 cohesive ends using dna polymerase i. the dna was then partially digested with micrococcal nuclease or pancreatic dnase. the products were separated by two-dimensional electrophoresis and characterized by composition, 3' ... | 1975 | 166999 |
| location and abundance of poly (a) sequences in sendai virus messenger rna molecules. | adenine-rich sequences from 18s sendai virus messenger rna species were 99% adenylate, 3'-oh terminal, and were present in at least 50% of the rna molecules. intact virus messenger rna molecules were resistant to exonucleolytic attack by polynucleotide phosphorylase, suggesting that their 3'-termini are masked. | 1975 | 167113 |
| buoyant density sedimentation of macromolecules in sodium iothalamate density gradients. | | 1975 | 167175 |
| effects of lead on the lipid composition of micrococcus luteus cells. | micrococcus luteus cells cultivated in medium containing lead salts exhibited a sequence of changes in the quantity of total cellular lipids with essentially no changes from normal cellular yields. the lipid composition of cells cultivated one to four times was moderately decreased (phase i) whereas that of cells cultivated five to six times was reduced by as much as 50% (phase ii). cells cultivated more than six times in lead-containing media had progressively greater quantities of lipid (phase ... | 1975 | 167661 |
| interactions of heteroaromatic compounds with nucleic acids. 2. influence of substituents on the base and sequence specificity of intercalating ligands. | this paper presents the results of a systematic study on the effects of substituents on the base and sequence specificity of tricyclic heteroaromatic compounds interacting with dna by intercalation. all the compounds tested are derived from proflavine and acridine orange analogs with different heteroatoms in the middle ring. their base and sequence specificities were determined by differential dialysis of the ligand against dna samples of differing g-c content. the main results indicate that (a) ... | 1975 | 168079 |
| the effect of methylene dimethanesulphonate (mdms) on the conformation of dna and its dependence on base composition. | the interaction of the alkanesulphonate, methylene dimethanesulphonate (mdms) with dna has been studied. thermal denaturation studies on mixtures of mdms and dna showed a dose-dependent decrease of the melting temperature midpoint (tm) of the dna. in addition, an irreversible decrease in ultraviolet absorption (hypochromism) preceded the hyperchromic shift, the magnitude of the former being linearly related to both the relative concentration of mdms and the g-c content of the dna used. neither t ... | 1975 | 168977 |
| effect of dna base composition on the intercalation of proflavine. a kinetic study. | the effect of dna base composition on the kinetics of the association between dna and proflavine has been investigated using the temperature jump relaxation method. it is found that, regardless of the g + c base composition the results fit a two step mechanism, the second of which exhibits characteristics of intercalation of proflavine into dna. however, they two equilibrium constants corresponding to these steps, ki and kii, depend on the nature of the dnas. the constant ki is found to be an or ... | 1975 | 169927 |
| infrared linear dichroism investigations of deoxyribonucleic acid complexes with poly(l-arginine) and poly(l-lysine). | complexes between dnas from various sources and poly(l-lysine) and poly(l-arginine) were studied by means of infrared linear dichroism. the measurements of dichroic ratios allowed us to determine the orientation of the phosphate group of dna in the complexes with basic polypeptides. at high relative humidities (higher than 90%, b form), the bisector of the less than opo in the complexes forms an angle with respect to the helical axis which has a value lower by about 4 degrees than in the corresp ... | 1975 | 170956 |
| a new, rapid, and sensitive assay for adenosine 5'-phosphosulphate (aps) kinase. | | 1975 | 171973 |
| competitive inhibition of transformation in group h streptococcus strain challis by heterologous deoxyribonucleic acid. | glucosylated deoxyribonucleic acid (dna) from phages t4 and t6 competes poorly with homologous dna causing only a slight decrease of transformation in group h streptococcus strain challis. other types of heterologous dnas (micrococcus luteus, clostridium perfringens, escherichia coli, calf thymus and non-glucosylated phage t6 dna), in contrast to glucosylated t4 and t6 dnas, compete with transforming dna to the normal, high extent. these results indicate that as in transformation of bacillus sub ... | 1975 | 172490 |
| [effect of polyakov's "prints" on the catalytic properties of an enzyme substrate system]. | silicagels, formed in presence of an enzyme (histidine decarboxylase from micrococcus sp. n.), substrate (l-histidine-hcl-h2o) and inhibitor (imidazole) (so-called polyakov's "print") exhibited specific catalytic properties in the reaction of l-histidine decarboxylation. the "prints" of the enzyme, inhibitor and substrate increased the activity of the enzyme-substrate system studied. the increased activity was probably due to the specific adsorption of imidazole ring of the substrate molecule by ... | 1975 | 175565 |
| replication intermediates of herpes simplex virus dna. | | 1976 | 176788 |
| amino acid sequence of paracoccus denitrificans cytochrome c550. | the amino acid sequence of paracoccus (formerly micrococcus) denitrificans cytochrome c550 has been established by a combination of standard chemical techniques and interpretation of a 2.5 a resolution x-ray electron density map. peptides derived from a trypsin digest were chemically sequenced, and then ordered by fitting them to the density map. the amino acid compositions of chymotryptic peptides confirmed the x-ray map ordering the tryptic peptides. the amino acid sequence of this respiratory ... | 1976 | 177408 |