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regulation of embryonic smooth muscle myosin by myosin light chain kinase and by protein kinase c. 19892533455
glomerulopathy in renal allografts from patients with and without active cytomegalovirus infection.cytomegalovirus (cmv) infection may affect renal graft survival, and a distinctive diffuse glomerulopathy has been described in renal grafts from patients with an active cmv infection. fifty renal graft biopsies taken 30 to 180 days post-transplant from patients with primary, reactivated, inactive and no cmv infection were examined retrospectively for evidence of this glomerulopathy without knowledge of cmv status. the lesion was found in seven of 30 patients with reactivated cmv infection, one ...19892546717
the process of baculovirus deproteinization.the localization and function of alkaline protease inside the supervirion capsids (granules, inclusion bodies) of granulosis virus and nuclear polyhedrosis virus have been determined with the help of electron microscopy. it has been shown that deproteinization of baculoviruses proceeds in several steps. the first two stages (the release of virus from supervirion capsids and the release of nucleocapsids from the internal and external coats, were accomplished in the intestine of the insects, while ...19892576597
[studies on anaerobic infection in oro-maxillary region--rapid diagnosis by gas-liquid chromatography and antibiotic susceptibilities of anaerobic bacteria].subject material for this study was pus collected from patients with purulent inflammation in the oro-maxillary region. direct gas-liquid chromatography (glc) analysis was made, bacterial isolation and identification were carried out, and comparisons were made with results from glc analysis and anaerobic isolates in a pyg medium. in addition, antibiotic susceptibilities of anaerobic bacteria were examined. results 1. anaerobic bacteria were isolated from 85 of 100 cases of obstructive abscesses. ...19892641196
quantitative bacteriology of a case of acute parotitis.it is generally assumed that the microorganisms responsible for acute suppurative parotitis are facultative anaerobes, primarily staphylococcus aureus and streptococcus viridans. a quantitative microbiologic investigation of an aspirate of pus from the case we report revealed a microbial flora (5.1 x 10(6) cfu/ml) consisting entirely of strict anaerobes (fusobacterium nucleatum and peptostreptococcus anaerobius). the strain of f. nucleatum was sensitive to metronidazole but resistant to penicill ...19892682427
serological studies of peptostreptococci using an indirect fluorescent antibody test.members of the genus peptostreptococcus are frequent isolates from periodontal lesions. a study was undertaken for determination of the serological relationships of oral and non-oral strains of several species of this genus. eighty-nine strains of peptostreptococci representing seven species were tested by means of an indirect fluorescent antibody technique (ifa), with rabbit antisera to peptostreptococcus anaerobius atcc 27337, peptostreptococcus micros vpi 2618-a, and peptostreptococcus produc ...19892685068
anaerobic bacteria in brain abscess, a review; observation of 18 consecutive cases.the aerobic and anaerobic bacterial flora of 18 consecutive cases of brain abscesses were studied. two cases were sterile. in five cases there was a single bacterial isolate. in five cases there was a single bacterial isolate the organism being staphylococcus aureus in two cases, bacteroides fragilis in two cases and peptostreptococcus anaerobius in one case. in the remaining eleven cases, more than one organism was isolated. a combination of anaerobe with an aerobe was observed in five cases an ...19892687167
disease response to tobacco mosaic virus in transgenic tobacco plants that constitutively express the pathogenesis-related pr1b gene.correlation of the temporal and spacial pattern of induction of the pathogenesis-related (pr) genes pr1a, pr1b, and pr1c with viral infections in certain tobacco cultivars has implicated pr proteins in viral disease resistance. to test whether the pr1 proteins of tobacco are involved in viral resistance, transgenic nicotiana tabacum plants were constructed which constitutively express the pr1b gene. this protein was secreted from cells of transgenic plants and accumulated in the extracellular sp ...19892815592
controlled evaluation of trypticase soy broth with and without gelatin and yeast extract in the detection of bacteremia and fungemia.the addition of gelatin to blood culture media has been suggested to prevent the inhibition of neisseria meningitidis, neisseria gonorrhoeae, gardnerella vaginalis, and peptostreptococcus anaerobius that is caused by sodium polyanetholsulfonate. to determine the effect of such supplementation on the overall yield of microorganisms, we compared the yield and speed of detection of clinically important microorganisms from 5422 paired 10-ml samples of blood cultured in trypticase soy broth (tsb) con ...19872831009
ecology of colorado tick fever. 19882849373
[role of alpha-1 and alpha-2-adrenoreceptors in achieving the compensatory response of the hemostasis system to plasmin]. 19882849566
antiviral chemoprophylaxis for cytomegalovirus infection. 19882849602
bevantolol hydrochloride--preclinical pharmacologic profile.bevantolol hydrochloride, a beta adrenoceptor antagonist, can be categorized using conventional schemes as being cardioselective, devoid of intrinsic sympathomimetic activity and having weak membrane-stabilizing and local anesthetic properties. the cardioselectivity of bevantolol was conferred by the incorporation of a 3,4-dimethoxyphenyl moiety into the terminal amino portion of the molecule. this portion of the molecule also appears to account for bevantolol's in vitro binding affinity at alph ...19862871786
beta b1 crystallin is an amine-donor substrate for tissue transglutaminase.the effects of tissue transglutaminase on the water-soluble proteins in bovine lens homogenates are described. addition of liver transglutaminase and ca2+ to calf lens homogenates resulted not only in the appearance of 50- and 57-kda dimers, but also in a decrease in the amount of beta b1 crystallin and the almost complete disappearance of beta b3 and beta a3. this is not the result of ca2+-induced proteolysis, since histamine completely inhibits this phenomenon. it may be concluded that these p ...19872887447
comparative efficacy of ceftriaxone and rifampicin in eradicating pharyngeal carriage of group a neisseria meningitidis.during an outbreak of meningococcal meningitis in saudi arabia, oral rifampicin (four doses in two days) was compared with a single intramuscular dose of ceftriaxone for prophylaxis in family contacts of patients with meningococcal disease. pharyngeal samples were taken for culture before and 1 and 2 weeks after administration. both follow-up cultures indicated that ceftriaxone was significantly more effective. at 1 week the eradication rates for ceftriaxone and rifampicin were 97% and 75%; at 2 ...19882897515
failure of expression of alpha-3-l-fucosyltransferase in human serum is coincident with the absence of the x (or le(x)) antigen in the kidney but not on leucocytes.the x antigen, beta gal(1----4)[alpha fuc(1----3)]beta glcnac-r, is mostly found in epithelial cells of the digestive tract, proximal convoluted tubules of the kidney, and granulocytes. the alpha-3-l-fucosyltransferase responsible for synthesis of this antigen is normally present in the serum, but we found 2 individuals, a mother and her daughter, who lack more than 90% of the serum fucosyltransferase. they are of african origin and are both le(a-b-). the young girl suffers from a congenital kid ...19882908489
oestrus phases of the syrian hamster. 19882976222
regulation by interleukin 2 of interleukin 2 receptors and gamma-interferon synthesis by human thymocytes: augmentation of interleukin 2 receptors by interleukin 2.the role of interleukin 2 (il 2) on the expression of il 2 receptors and on the synthesis of gamma-interferon (gamma-ifn) by human thymocytes was investigated. human thymocytes isolated from specimens obtained during cardiac surgery of infants and children were induced with one or all of the following agents: il 2, concanavalin a (con a), and 12-o-tetradecanoylphorbol 13-acetate (tpa). the expression of il 2 receptors and gamma-ifn titers were determined. the results indicate that thymocytes cul ...19852981271
effect of sodium polyanetholesulfonate and gelatin on the recovery of gardnerella vaginalis from blood culture media.sodium polyanetholesulfonate (sps) is used as a routine supplement to blood culture media to enhance recovery of microorganisms, but it inhibits the growth of peptostreptococcus anaerobius, neisseria meningitidis, neisseria gonorrhoeae, and streptobacillus moniliformis. comparative clinical blood culture studies at the university of colorado hospital suggested that sps also inhibits the growth of gardnerella vaginalis. we inoculated 16 blood culture isolates of g. vaginalis into 11 blood culture ...19852987298
sulbactam/ampicillin versus metronidazole/gentamicin in the treatment of severe pelvic infections.the clinical efficacy and safety of sulbactam/ampicillin versus metronidazole/gentamicin were compared in 39 patients with severe pelvic infections. 30 patients had severe acute pelvic inflammatory disease with peritonitis, 3 tubo-ovarian abscesses, 4 endomyometritis, and 2 posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures from the sites of infection yielded 259 micro-organisms from 38 patients; an average of 6.8 bacteria per infection (3.9 anaerobes and 2.9 aerobes). the most f ...19863013566
ampicillin/sulbactam versus metronidazole-gentamicin in the treatment of soft tissue pelvic infections.the clinical efficacy and safety of ampicillin/sulbactam versus metronidazole-gentamicin were evaluated in a comparative, randomized, prospective study. forty-four patients were enrolled: 22 received the ampicillin/sulbactam regimen, and 22 received the metronidazole-gentamicin combination. there were 33 cases of severe acute pelvic inflammatory disease, two tuboovarian abscesses, five cases of endomyometritis, and two cases of posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures f ...19873030109
cloning and expression of ap-2, a cell-type-specific transcription factor that activates inducible enhancer elements.human ap-2 is a sequence-specific dna-binding protein that interacts with inducible viral and cellular enhancer elements to stimulate transcription of selected genes. here, we report the isolation and characterization of a human cdna clone containing the entire protein-coding region of ap-2. the deduced primary amino acid sequence of ap-2 does not contain a domain resembling any previously identified dna binding motif. however, an interesting feature of the ap-2 protein is a clustered arrangemen ...19883063603
cloning and expression of ap-2, a cell-type-specific transcription factor that activates inducible enhancer elements.human ap-2 is a sequence-specific dna-binding protein that interacts with inducible viral and cellular enhancer elements to stimulate transcription of selected genes. here, we report the isolation and characterization of a human cdna clone containing the entire protein-coding region of ap-2. the deduced primary amino acid sequence of ap-2 does not contain a domain resembling any previously identified dna binding motif. however, an interesting feature of the ap-2 protein is a clustered arrangemen ...19883063603
effects of alpha-methyl-p-tyrosine on monoamines and catecholamine receptors in rat cerebral cortex and neostriatum.the effects of inhibiting the synthesis of catecholamines using alpha-methyl-p-tyrosine (alpha-mpt) were investigated in four cortical regions (cingulate, somatosensory, visual and entorhinal-piriform) as well as in the neostriatum (caudate-putamen). after acute (48 hours) treatments with alpha-mpt the endogenous na levels were significantly reduced in all regions examined. the da contents were also decreased in regions known to possess a dense dopaminergic innervation (neostriatum, cingulate an ...19883067104
regional localization of cd18, the beta-subunit of the cell surface adhesion molecule lfa-1, on human chromosome 21 by in situ hybridization.the gene coding for the beta-subunit of the cell surface adhesion glycoprotein lfa-1 has been localized to the tip of the long arm of chromosome 21 at 21q22.1-qter.19883073708
characterization of cross-reactive proteins detected by culturette brand rapid latex test for clostridium difficile.clostridium sporogenes, peptostreptococcus anaerobius, and bacteroides asaccharolyticus have been reported to react in the culturette brand rapid latex test (marion scientific, div. marion laboratories, inc., kansas city, mo.) for clostridium difficile. from the results of this study we showed that c. sporogenes and p. anaerobius produce a protein which is very similar biochemically and immunologically to the protein of c. difficile that is detected by the test. thus, the positive latex reaction ...19883128574
biosynthesis of benzylpenicillin by penicillium chrysogenum and its golgi apparatus.the fine structure of high and low-yield mutants of penicillium chrysogenum producing 10,000 and 100 units of benzylpenicillin was compared. the cells of both mutants showed typical eukaryotic ultrastructure. the golgi vesicles, present in largest number in cells of high-yield mutant, fuse with the cell membrane and play an important role in the transport of benzylpenicillin from the cytoplasm to the cell environment. benzylpenicillin was localized in cells of the high-yield mutant by means of e ...19873138964
bactericidal effects of photoradiation therapy with hematoporphyrin derivative.hematoporphyrin derivative (hpd) localizes selectively in malignant and rapidly metabolizing tissues and undergoes a cytotoxic reaction when exposed to light of a specific wavelength. hpd has been studied extensively with regard to the diagnosis and treatment of tumors but not with regard to bactericidal activity. this investigation assessed the effect of light-activated hpd on various microorganisms, on human polymorphonuclear leukocytes, and on the interactions of polymorphonuclear leukocytes ...19853155547
preformed enzyme profiles of reference strains of gram-positive anaerobic cocci.the preformed (constitutive) enzyme profiles of 30 type strains and reference strains of gram-positive anaerobic cocci were determined with two commercial systems, rapid ana and a prototype system from api. both systems identified peptostreptococcus anaerobius, ps. asaccharolyticus, ps. indolicus, ps. magnus and ps. micros accurately, except for one strain of ps. magnus misidentified as ps. micros by the rapid ana system. the indole-negative, butyrate-producing cocci (classified at present as ps ...19883172172
tetracycline resistance and tetm in oral anaerobic bacteria and neisseria perflava-n. sicca.tetracycline-resistant organisms isolated from six patients with periodontal disease included bacteroides spp., eubacterium spp., fusobacterium nucleatum, neisseria perflava-n. sicca, peptostreptococcus anaerobius, veillonella parvula, and facultative streptococci. all but the bacteroides spp. and eubacterium spp. hybridized with the tetm determinant. an additional 417 bacterial strains were screened, and 4% of both the oral streptococci and the fusobacterium spp. hybridized with the tetm probe.19883190205
individual free fatty acids and lactate uptake in the human heart during severe sepsis.coronary haemodynamics and myocardial metabolism of nonesterified fatty acids (nefa) and lactate were studied in 11 patients with severe sepsis, and compared to 10 control subjects. coronary sinus blood flow was evaluated by thermodilution. arterial and coronary sinus blood samples were collected for the measurement of lactate and total and individual nefa concentrations both in septic and control patients. there was an increase in lactate and total nefa arterial concentrations with a marked inc ...19883232957
evaluation of a commercial latex test for clostridium difficile for reactivity with c. difficile and cross-reactions with other bacteria.seventy-eight species of bacteria (739 isolates) were tested for reactivity with a commercial latex test for clostridium difficile. all noncytotoxic as well as cytotoxic strains of c. difficile reacted positively. immuno-specific cross-reactions were found only with c. sporogenes, proteolytic c. botulinum, and peptostreptococcus anaerobius.19883235677
bacteriology of periodontal disease in the cat.subgingival plaque samples were obtained from 32 cats showing different stages of periodontal disease. correlations were sought between gingival index scores and the prevalence of various microbial groups, and between microbial populations found in sites designated as most-affected and least-affected within individual cats. the tendency with higher gingival index scores, and with the most-affected sites, was toward a microbial population composed to a greater extent of anaerobic gram-negative ro ...19883245794
surgical cannulation of a hepatic vein in dairy cows utilizing diagnostic ultrasound.use of a diagnostic ultrasonography unit in the placement of hepatic vein catheters is described. diagnostic ultrasound proved to be a safe and rapid means of identifying the hepatic venous circulation. it provided a clear visual guide in an otherwise blind surgical procedure and allowed for a precise determination of catheter placement. thus, the method could be used to advantage in other types of catheterizations. use of ultrasonography reduced the duration of surgery by 50 to 75%, thereby min ...19873294948
identification of gram-positive anaerobic cocci by use of systems for detecting pre-formed enzymes.two systems for detecting pre-formed enzymes, rapid ana and a prototype system from api, were compared in a blind study for their ability to identify 69 gram-positive anaerobic cocci isolated from clinical specimens. both systems were able to identify peptostreptococcus anaerobius, ps. asaccharolyticus and ps. micros accurately without the need for further tests. the prototype api system identified all isolates of ps. magnus correctly, but the rapid ana system misidentified several isolates as p ...19883357195
piezoelectric polymer curvature sensor for measurement of regional curvature radius of lv wall.to evaluate regional myocardial function, we developed a curvature sensor for direct and instantaneous measurement of the regional curvature radius of the lv wall. the sensor is a bimorph of two sheets of thin piezoelectric polymer film. the relation between output voltage of the sensor and the reciprocal of the known curvature radius has been shown to be linear. in anesthetized dogs, we inserted the curvature sensor into the subepimyocardium with a specially designed introducer. special care wa ...19883364584
effects of temperature on the oxygen consumption in thin slices from different brain regions.the oxygen consumption of brain slices from 12 different regions of the guinea pig brain was measured and the effects of temperature on the consumption was investigated. at 37 degrees c the oxygen consumption in cerebral gray matter and inferior colliculus was higher than that of other regions, whilst the consumption in cerebral white matter and brainstem was approximately half of that in cerebral gray matter. in any region lowering the tissue temperature from 37 to 28 degrees c reduced the oxyg ...19883380322
recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals.examination of 15,844 clinical specimens submitted over 12 years (1973 to 1985) to the anaerobic microbiology laboratories in two military hospitals demonstrated the recovery of anaerobic bacteria in 4,458 (28.1%) specimens. the specimens yielded 6,557 anaerobic isolates (1.47 isolates per specimen). bacteroides spp. accounted for 43% of all isolates; anaerobic gram-positive cocci, 26%; clostridium spp., 7%; and fusobacterium spp., 4%. bacteroides spp. predominated in abscesses, obstetrical and ...19883384929
induction of gene amplification by arsenic.arsenic is a well-established carcinogen in humans, but there is little evidence for its carcinogenicity in animals and it is inactive as an initiator or tumor promoter in two-stage models of carcinogenicity in mice. two arsenic salts (sodium arsenite and sodium arsenate) induced a high frequency of methotrexate-resistant 3t6 cells, which were shown to have amplified copies of the dihydrofolate reductase gene. the ability of arsenic to induce gene amplification may relate to its carcinogenic eff ...19883388020
evidence of estrogen receptors in normal human osteoblast-like cells.in seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. the nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. these are characteristics of biologically active estrogen receptors. pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six ce ...19883388021
[the differentiation of bordetella bronchiseptica strains. 7. phenotypic changes in bordetella bronchiseptica through changes of phase]. 19883395180
embryonic stem cell-mediated transfer and correct regulation of the chicken delta-crystallin gene in developing mouse embryos.to study regulation of delta-crystallin expression during ontogeny, we transferred the gene from chicken into developing mouse embryos by first transforming an embryonic stem (es) cell line of mouse and then producing chimaeric embryos by combining them with normal mouse embryos. using this technique, genes were transferred into a variety of developing mouse tissues with high efficiency. two delta-crystallin gene constructs were used: the wild-type gene with 2200 bp of the 5' flanking sequence, ...19883416775
studies on the mechanics of the normal human middle ear.the middle ear was studied in temporal bone preparations using a laser-doppler interferometer. for measurements at a sound level of 80 db spl this method proved to be very reliable, as was shown by good reproducibility of results in experiments over more than 6 hours. the vibrations of the tympanic membrane and stapes footplate were studied from 200 hz to 10 khz and the results demonstrate a piston-like movement of the stapes footplate up to 120 db spl. the damping effect of the normal ear is lo ...19863536194
comparative activity of metronidazole and tinidazole against clostridium difficile and peptostreptococcus anaerobius.broth dilution mics of metronidazole against 38 strains of clostridium difficile (0.13 microgram/ml) and 11 strains of peptostreptococcus anaerobius (0.11 microgram/ml) were lower than those of tinidazole (0.16 and 0.17 microgram/ml, respectively). as molar concentrations the nitroimidazoles were equally active. the mics of the two drugs correlated positively, and the relative activity of tinidazole increased with decreasing susceptibility of both species.19873566248
controlled evaluation of modified radiometric blood culture medium supplemented with gelatin for detection of bacteremia and fungemia.although the addition of 1.2% gelatin to broth blood culture media containing sodium polyanetholesulfonate has been shown to enhance detection of certain bacteria, including neisseria meningitidis, n. gonorrhoeae, peptostreptococcus anaerobius, and gardnerella vaginalis, the effect of such supplementation on the detection of other microorganisms causing bacteremia and fungemia is not known. therefore, we studied bactec 6b medium with and without gelatin in 6,833 paired comparisons to examine the ...19873624437
[clinical studies on cefuzonam in obstetrics and gynecological infections].to evaluate the usefulness and the safety of cefuzonam (czon, l-105) against obstetric and gynecological infections, we conducted clinical trials and obtained the results summarized as follows. 1. thirteen patients with obstetric and gynecological infections (intrauterine infection 2 cases, adnexitis 7, 1 case each of abscess of adnexa uteri, abscess of the vaginal wall, pyelonephritis, and mammitis) were treated with czon. the czon was administered by intravenous injection or intravenous drip i ...19873669283
[housing and health of farm animals].data on the relationship between housing systems and health in farm animals are relatively scarce. in dairy cattle and poultry, for which special housing systems are dominant in the netherlands, no important new figures are available. in veal calves, the group housing system is being investigated. preliminary results indicate that there are many problems in the first 6 to 8 weeks. more research has been done on pigs. it is now clear that the floor system is important not only with respect to leg ...19873672468
identification of anaerobic bacteria using high-performance liquid chromatography.20 species of the genera clostridium, bacteroides, fusobacterium and the type strains of peptostreptococcus anaerobius, peptococcus asaccharolyticus, veillonella parvula and propionibacterium acnes were examined for the production of volatile (vfa) and nonvolatile (nvfa) short-chain fatty acids using high-performance liquid chromatography (hplc) with a column for organic acids (aminex hpx-87h). 10 min are needed for sample preparation and the vfa and nvfa were detected simultaneously in a single ...19873673341
practolol inhibits human skin fibroblast cell mat hydroxyproline accumulation.despite being poorly absorbed practolol (n-4-2-hydroxy-3-(1-methyl-ethyl)-amino propoxy phenyl acetamine) inhibited the accumulation of cell mat hydroxyproline, a measure of collagen synthesis, by human skin fibroblasts (dt2ph) in vitro, (id50 0.8 x 10(-3) m). the degree of inhibition was dependent on the concentration of practolol used and the incubation time. neither preinitiation of collagen synthesis nor omitting ascorbic acid from the incubation medium modified this inhibitory action. in co ...19873687592
immunochemical characterization of the cell surface carbohydrate antigens of peptostreptococcus anaerobius.two carbohydrate antigens were isolated from the cell surface of peptostreptococcus anaerobius. one, extracted from purified cell walls with naoh, contained glucose and phosphorus, with traces of galactosamine and glucosamine. serological activity was detected by a 'dot blot' procedure. the second antigen, extracted from cell membranes with phenol and purified by chromatography on sepharose 6b and an immunoadsorbent column, contained glucose, glycerol phosphate, phosphorus and fatty acids. antig ...19863711862
bvd virus infection and embryo transfer. 19863716157
proline requirement for glucose utilization by peptostreptococcus anaerobius atcc 27337.resting cells of peptostreptococcus anaerobius maintained under anaerobic conditions were unable to metabolize either glucose or alanine. the addition of proline to the appropriate suspension, however, resulted in the immediate utilization of both compounds. fermentation of alanine by the cells required that stoichiometric concentrations of proline be present in the medium; and during the oxidation of alanine, proline was simultaneously reduced to the ring cleavage product delta-aminovaleric aci ...19873804441
analysis of edta-soluble cell surface components of gram-positive anaerobic cocci.the protein content of edta extracts from 76 strains of gram-positive anaerobic cocci was examined using sds-page. strains of peptostreptococcus anaerobius produced almost identical profiles; greater heterogeneity was observed within the species peptococcus magnus, peptococcus prevotii and peptococcus asaccharolyticus, but several strains within each biotype produced similar patterns. serological investigation of these extracts by elisa revealed numerous cross-reactions among the different bioty ...19863806050
[polarographic method for determining activity of dehydrogenases of the pentosephosphate pathway of carbohydrate metabolism].a procedure is developed for estimation of glucose-6-phosphate and 6-phosphogluconate dehydrogenases activity in human erythrocytes. the procedure involved polarographic registration of oxygen consumption, which is directly related to content of nadph formed, in a coupled enzymatic system containing peroxidase and mn2+.19863811277
serum urate concentrations in the dalmatian coach hound.the mean concentration of urate in the serum of 80 dalmatian coach hounds was approximately double that in the serum of 99 dogs of other breeds. both values were higher than those reported previously.19853837799
a review of the enzymic errors in the various porphyrias. 19853892647
moxalactam versus clindamycin plus tobramycin in the treatment of obstetric and gynecologic infections.the clinical efficacy of moxalactam versus clindamycin/tobramycin was evaluated in a comparative, randomized, prospective study. sixty patients were treated: 30 with moxalactam and 30 with clindamycin/tobramycin. there were 15 cases of tuboovarian abscess, 36 cases of severe pelvic inflammatory disease with peritonitis, eight cases of endomyometritis, and one wound abscess. aerobic and anaerobic cultures from the sites of infection yielded 441 microorganisms from 53 patients; an average of 8.3 b ...19853895947
the classification and identification of the anaerobic gram-positive cocci.the approved lists of bacterial names (1980) includes the following peptococcus and peptostreptococcus species pathogenic for man: peptococcus magnus, p. prevotii, p. asaccharolyticus, peptostreptococcus anaerobius, p. micros and p. productus. a presumptive identification of these species can be made on the basis of cellular morphology, susceptibility to novobiocin, characterization of metabolic end products, fermentation of glucose and lactose, and production of indol. capnophilic and microaero ...19853906854
antimicrobial susceptibilities of anaerobic bacteria isolated from female genital tract infections.certain species or subspecies of anaerobic bacteria are isolated with higher frequency from female genital tract infections than from other anatomic sites. to gain susceptibility data more specific to the treatment of these infections, nine antimicrobial agents were tested by an agar dilution technique against 230 anaerobic bacteria isolated solely from obstetric and gynecological infections. these genital isolates were, in general, very susceptible to imipenem (most active, inhibiting all gram- ...19853994347
change in the volatile fatty acids content of laboratory stored sterilized and non-sterilized swine wastes.the influence of urine and microbial activity was studied as possible factors leading to the generation of volatile fatty acids in non-sterilized stored swine wastes. urine was found to promote the generation of the volatile acids. the microbial origin of the volatile acids in the wastes was ascertained by the growth of an anaerobic bacterial strain (peptostreptococcus anaerobius). significant generation and transformation of these acids was observed in sterilized swine waste samples stored unde ...19854033458
fluorescent antibody study of the gram-positive anaerobic cocci.fluorescent antibody conjugates were prepared from five species of anaerobic cocci commonly isolated from human infections. when tested with homologous and heterologous cells these conjugates were found to be highly specific. there was no evidence of a common genus antigen. peptococcus magnus conjugates detected a species-specific antigen; cross-reactions with peptostreptococcus anaerobius were readily eliminated by absorption. the conjugates from peptococcus asaccharolyticus, peptococcus prevot ...19744140708
striatal tyrosine hydroxylases with high and low affinity for tyrosine: implications for the multiple-pool concept of catecholamines. 19734148015
[the gas supply of hydrogen bacteria in submerged culture]. 19724196475
[influence of a dietary excess of sodium chloride on the 5-hydroxytryptamine level of the brain and the epiphyses in rats]. 19664225972
the golgi complex in the esophageal mucous glands of the newly hatched chick. 19714257786
[the replicative complexes of sendai virus]. 19714332921
bactericidal activity of aerobic and anaerobic polymorphonuclear neutrophils.human polymorphonuclear neutrophils (pmn) were made anaerobic by nitrogen washout (oxygen saturation <1%, eh < -42 mv at ph 7.0), and the ability of the cells to kill bacteria was assayed and compared to the bactericidal activity of aerobic pmn. anaerobic pmn were able to kill staphylococcus epidermidis, enterococcus, viridans streptococci, pseudomonas aeruginosa, peptostreptococcus anaerobius, bacteroides fragilis, clostridium perfringens, and peptococcus magnus normally. organisms that were no ...19744361295
enhancement of the sensitivity of the immunodiffusion reaction in the detection of antibody against bovine adenoviruses. 19734365196
[relations between plague bacteria and erysipelothrix under various environmental conditions]. 19744462011
social factors affecting the development of mounting behavior in male rhesus monkeys. 19744475645
modification by orthophosphate of the transfer of tooth phosphorus into cariogenic streptococci. 19724500617
suppressor cells in the rabbit appendix. 19744547314
studies of cardiac allografts in syrian hamsters. 19724557240
ftc-insulin conjugates. i. preparation of fluorescein thiocarbamyl-insulin conjugates. 19734579445
serological relationship of swine vesicular disease virus and coxsackie b5 virus. 19734586437
the linkage relationship of the cytoplasmic drug-resistance factors in saccharomyces cerevisiae. 19744594012
sodium polyanethol sulfonate sensitivity of anaerobic cocci.growth of peptostreptococcus anaerobius was shown to be totally inhibited by sodium polyanethol sulfonate (sps). other anaerobic cocci grew in the presence of sps although some strains of peptococcus prevotii and peptococcus magnus showed delayed growth. a sps disk assay for the presumptive identification of p. anaerobius is described.19744598436
[impulsation of the splanchnic and vagus nerves following introduction of fat into the lumen of the small intestine]. 19724648063
preliminary characterization of the 5 -dihydrotestosterone binding protein in the epididymal cytosol fraction. in vivo studies. 19724678201
bovine abortion associated with candida tropicalis. 19734692299
[hypothermic pulsatile perfusion of dog kidneys: 96-hour preservation by perfusate exchange]. 19734696656
[treatment of schistosoma hematobium infestation with niridazol]. 19734730988
[steroid biosynthesis and secretion by the human testis]. 19734757400
cell-free synthesis of myosin by cardiac myofibrillar ribosomes. 19734767316
[the effect of cocarboxylase on lymphocyte metabolism in newborn rats with experimental asphyxia]. 19734804642
[unitary discharge of pontine reticular formation and phasic ponto-geniculo-occipital activity in the cat under reserpine]. 19694895788
an immunological comparison of central and peripheral nervous tissues. 19674960240
flow and composition of lymph collected from the skeletal muscle of the rabbit hind limb. 19725085348
neutrophil chemotaxis induced by anaerobic bacteria isolated from necrotic dental pulps.anaerobic bacteria belonging to the species bacteroides melaninogenicus, bacteroides asaccharolyticus, fusobacterium nucleatum, peptostreptococcus anaerobius and eubacterium alactolyticum, isolated from necrotic dental pulps, were tested for their capacity to induce neutrophil leukocyte chemotaxis. generation of chemotactic factors in serum by whole bacterial cells and chemotaxis induced by bacterial extracts were studied in vitro by the boyden technique. subcutaneously implanted wound chambers ...19806103573
metabolic inhibition of peptostreptococcus anaerobius decreases the bactericidal effect of hydrogen peroxide.peptostreptococcus anaerobius vpi 4330-1 was tested under various conditions for examination of the bactericidal effect of hydrogen peroxide. the cells were most rapidly killed by hydrogen peroxide when they were in the exponential-growth phase. cooling or starving the cells decreased the bactericidal effect of hydrogen peroxide. the ionophore nigericin and the metal ion chelating agent 2,2'-bipyridine stopped macromolecular syntheses and greatly decreased the bactericidal effect of hydrogen per ...19816173014
the preparation and characterization of cr(iii) and co(iii) complexes of gdp and gtp and their interactions with avian phosphoenolpyruvate carboxykinase.the exchange inert coordination complexes, cr(h2o)4gdp, cr(h2o)4gtp, cr(nh3)4gdp, cr(nh3)4gtp, co(nh3)4gdp, and co(nh3)4gtp have been synthesized and characterized. the lambda and delta coordination isomers of cr(h2o)4gdp, cr(nh3)4gdp, and the four cr(h2o)4gtp isomers have been separated by reverse phase hplc and characterized by their cd spectra. while the isomers of co(nh3)4gtp have not been successfully separated, 31p nmr spectroscopy reveals the presence of the lambda and delta forms. the co ...19883346664
human and bovine endothelial cells synthesize membrane proteins similar to human platelet glycoproteins iib and iiia.human umbilical vein endothelial (huve) and bovine aortic endothelial (bae) cells in culture were examined to determine whether membrane proteins similar to human platelet glycoproteins (gp) iib and iiia were present. the huve and bae cells were either 125i-surface labeled or metabolically labeled. triton x-100 lysates of labeled cells were immunoprecipitated with polyclonal antibodies prepared against purified human platelet gp iib-iiia complex. two membrane proteins were detected on both huve ...19853161885
[antibody-dependent harmful effect of non-immune spleen cells in acute experimental tick-borne encephalitis in mice].the injection of nonprotective dilutions of immune serum and nonimmune spleen cells into mice infected with tick-borne encephalitis virus induced a sharply pronounced immunopathological effect: the mean survival time of the recipients decreased by 3.6 days in comparison with the control animals. this effect was not linked with the increased replication of the virus in the brain. the antibody-dependent damaging action of spleen cells could be reproduced by using the cells of both syngeneic and al ...19892786665
adverse effects of the use of unusual phenethylamine compounds sold as illicit amphetamine.a 40-year-old man, who had regularly taken illicit amphetamine by intra-nasal inhalation for several years without ill effects, was admitted to hospital with signs of massive adrenergic overstimulation shortly after inhaling material which he had purchased in the belief that it was amphetamine. the administration of the beta-blocker practolol produced a paradoxical increase in blood pressure. after his discharge from hospital he suffered disabling feelings of anxiety for several weeks. analysis ...19892770471
purification and properties of a protein activator of phosphorylated branched-chain 2-oxo acid dehydrogenase complex.the protein activator of phosphorylated branched-chain 2-oxo acid dehydrogenase complex was purified greater than 1000-fold from extracts of rat liver mitochondria; the specific activity was greater than 1000 units/mg of protein (1 unit gives half-maximum re-activation of 10 munits of phosphorylated complex). sodium dodecyl sulphate/polyacrylamide-gel electrophoresis gave two bands (mr 47700 and 35300) indistinguishable from the alpha- and beta-subunits of the branched-chain dehydrogenase compon ...19853977842
high resolution pattern of an inverted duplication (15).the nature and the origin of a supernumerary marker chromosome in a mentally retarded boy were determined by various staining techniques on metaphase chromosomes and by gbg high resolution technique. the karyotype was found to be 47,xy, + inv dup(15) (pter----q13: :q13----pter) and the supernumerary chromosome was of maternal origin.19863457663
inactivation of the polyanionic detergent sodium polyanetholsulfonate by hemoglobin.sodium polyanetholsulfonate (sps) has been added to blood culture media for many years. its incorporation results in a higher yield of positive blood cultures due to its inactivation of antimicrobial cationic compounds. the most active of these cations include complement components, aminoglycoside-aminocyclitol antibiotics, and receptors on polymorphonuclear leukocytes. there have been reports from studies conducted outside patient blood culture bottles that sps itself may possess antibacterial ...19836315765
[cefotiam passage into the maxillary sinus tissues].clinical study was made on cefotiam (ctm) and the following results were obtained. tissue concentrations of ctm were determined 1 hour after intravenous injection (ctm 1 g) in chronic sinusitis and maxillary cyst. concentrations of ctm were 16.53 micrograms/g, 13.26 micrograms/g in mucosa of the maxillary sinus, maxillary cyst, respectively. antibacterial activity of ctm was measured on escherichia coli, staphylococcus epidermidis, aerococcus, acidaminococcus fermentans, peptostreptococcus anaer ...19836325743
the survival of anaerobic bacteria at 4 degrees c and 22 degrees c on swabs in three transport systems. statistical evaluation by application of a variance component model.the survival of anaerobic bacteria on swabs in three transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), a plain cotton swab in a dry aerobic tube (da) and a plain cotton swab in an aerobic agar tube (aa). the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum and clostridium ra ...19836346789
the recovery of anaerobic bacteria from swabs in three transport systems. statistical evaluation by application of a variance component model.the recovery of six anaerobic species from swabs in three transport systems was quantitatively evaluated. the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum, and clostridium ramosum. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), or a plain cotton swab in either a dry aerobic tube (da) or an aerobic agar tube (aa). swabs were charged ...19836346790
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