| peptococcla urinary tract infection with bacteremia: a case report. | the second documented case of urinary tract infection with associated bacteremia of peptococcus asaccharolyticus origin is reported. high fever and low peripheral white blood count were manifestations of bacteremia with pepococcus asaccharolyticus, an anaerobic gram-positive coccus. although anaerobes have been documented to cause urinary tract infections resulting in bacteremia most previous reports implicate aerobes in these processes. the problems inherent in culturing urine for anaerobes are ... | 1980 | 7411710 |
| effect of temperature, humidity and exposure to oxygen on the survival of anaerobic bacteria. | the relative effect of humidity, temperature and exposure to oxygen on the survival of 11 strains of bacteriodes spp., fusobacterium necrophorum, eubacterium lentum, clostridium ramosum, peptococcus asaccharolyticus and peptostreptococcus anaerobius was evaluated. suspensions of the test strains were applied to membrane filters either in empty sterile petri dishes or on plates of non-nutrient agar to provide dry or moist conditions. the filters were stored aerobically or anaerobically in an atmo ... | 1980 | 7431377 |
| urinary tract infection caused by anaerobic bacteria in children. | anaerobic bacteria were recovered from 5 children with urinary tract infection (uti). three had pyelonephritis and 2 cystitis. two of the patients had a history of prior recurrent uti. urine samples were collected using suprapubic aspiration. the anaerobic organism recovered were 3 isolates of bacteroides fragilis and one each of b. melaninogenicus, peptococcus asaccharolyticus, and bifidobacterium adolescentis. mixed infection was present in 3 children. in 2 cases b. fragilis were present with ... | 1980 | 7445306 |
| techniques for controlling variability in gram staining of obligate anaerobes. | identification of anaerobes recovered from clinical samples is complicated by the fact that certain gram-positive anaerobes routinely stain gram negative; peptostreptococcus asaccharolyticus, eubacterium plautii, clostridium ramosum, clostridium symbiosum, and clostridium clostridiiforme are among the nonconformists with regard to conventional gram-staining procedures. accurate gram staining of american type culture collection strains of these anaerobic bacteria is possible by implementing fixin ... | 1995 | 7538512 |
| ecology of colorado tick fever. | | 1988 | 2849373 |
| comparison of native and mutant proteins provides a sequence-specific assignment of the cysteinyl ligand proton nmr resonances in the 2[fe4s4] ferredoxin from clostridium pasteurianum. | a sequence-specific assignment is presented for the eight low-field paramagnetically shifted cysteinyl ligand proton nmr resonances in the 2[fe4s4] ferredoxin from clostridium pasteurianum. the assignment is based upon comparison of chemical shifts in 1d and 2d nmr spectra of native oxidized protein and those of three mutants. the mutant proteins g12a and g41a were designed to produce minor local structural changes (hence small chemical shift perturbations) in either cluster i (glycine 12 to ala ... | 1994 | 7981209 |
| iron-sulfur cluster-containing l-serine dehydratase from peptostreptococcus asaccharolyticus: correlation of the cluster type with enzymatic activity. | investigations were performed with regard to the function of the iron-sulfur cluster of l-serine dehydratase from peptostreptococcus asaccharolyticus, an enzyme which is novel in the class of deaminating hydro-lyases in that it lacks pyridoxal-5'-phosphate. anaerobically purified l-serine dehydratase from p. asaccharolyticus revealed epr spectra characteristic of a [3fe-4s]+ cluster constituting 1% of the total enzyme concentration. upon incubation of the enzyme under air the intensity of the [3 ... | 1994 | 8082806 |
| model of a complex between the tetrahemic cytochrome c3 and the ferredoxin i from desulfovibrio desulfuricans (norway strain). | a three-dimensional model of an electron-transfer complex between the tetrahemic cytochrome c3 and the ferredoxin i from the sulfate-reducing bacterium desulfovibrio desulfuricans (norway strain) has been generated through computer graphics methods. the model is based on the known x-ray structure of the cytochrome and on a model of the ferredoxin that has been derived through computer graphics modeling and energy minimization methods, from the x-ray structure of the homologous ferredoxin from pe ... | 1988 | 2847143 |
| 16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov. | the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ... | 1994 | 8123556 |
| [oncogenes in human leukemia]. | results of our study on the activation of n-ras oncogene by point mutation in human leukemia and myelodysplastic syndrome have been described in this article. point mutation was observed mainly on the 12th, 13th and 61st amino acid codon of ras genes. therefore, oligomers containing mutations at these codons were used as probes for dot blot analysis of dna derived from patient's bone marrow cells or leukemia cells. polymerase chain reaction technique was used to amplify the dna of ras genes cont ... | 1989 | 2650633 |
| bacterial l-serine dehydratases: a new family of enzymes containing iron-sulfur clusters. | two families of enzymes are described which catalyse identical chemical reactions but differ in their prosthetic groups and hence in their mechanism of action. one family, the pyridoxal-5'-phosphate (plp)-dependent l-threonine dehydratases, also use l-serine as substrate. the other, hitherto unrecognized family is the iron-dependent, highly specific bacterial l-serine dehydratases. it has been shown that l-serine dehydratase from the anaerobic bacterium peptostreptococcus asaccharolyticus contai ... | 1993 | 8236444 |
| [gardnerella vaginalis in infections of reproductive organs]. | the study was aimed at multidirectional studies on bacteria isolated from smears from vagina and cervix uteri in 226 patients with inflammatory states of their reproductive organs. most frequently isolated aerobic bacteria were gram-negative--27%, mainly e. coli, and enterococcus faecalis--in 18% of cases. anaerobic bacteria were isolated in 81% of cases: gardnerella vaginalis was isolated in 28% and lactobacillus in 53% of cases. other anaerobic bacteria were: peptococcus asaccharolyticus (15.5 ... | 1993 | 8309297 |
| peritonsillar abscess, retropharyngeal abscess, mediastinitis, and nonclostridial anaerobic myonecrosis: a case report. | peritonsillar abscess is a potentially life-threatening complication of acute tonsillitis. on occasion, peritonsillar abscess can extend to neck spaces and/or to the mediastinum. we describe a case of a patient with a peritonsillar abscess that extended to the neck, producing bilateral retropharyngeal abscesses and myonecrosis of the strap muscles. culture of a specimen of the necrotic muscle yielded prevotella intermedia, prevotella buccae, lactobacillus catenaforme, another lactobacillus speci ... | 1993 | 8324135 |
| l-serine and l-threonine dehydratase from clostridium propionicum. two enzymes with different prosthetic groups. | l-serine dehydratase from the gram-positive bacterium peptostreptococcus asaccharolyticus is novel in the group of enzymes deaminating 2-hydroxyamino acids in that it is an iron-sulfur protein and lacks pyridoxal phosphate [grabowski, r. and buckel, w. (1991) eur. j. biochem. 199, 89-94]. it was proposed that this type of l-serine dehydratase is widespread among bacteria but has escaped intensive characterization due to its oxygen lability. here, we present evidence that another gram-positive ba ... | 1993 | 8344301 |
| in vitro activity of fk037, a new parenteral cephalosporin, against anaerobic bacteria. | the activity of fk037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. a fastidious facultative anaerobe, gardnerella vaginalis, was also studied. fk037 inhibited 90% of isolates of peptostreptococcus anaerobius, peptostreptococcus asaccharolyticus, clostridium perfringens, mobiluncus spp., g. vaginalis, and porphyromonas gingivalis at < or = 0.78 micrograms/ml. the mics of fk037 for 50 an ... | 1993 | 8517721 |
| the in vitro activity of trospectomycin against anaerobic bacteria. | the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ... | 1995 | 8667035 |
| [anaerobic bacteria in chronic renal insufficiency: apropos of a case]. | we relate a case of chronic renal insufficiency complicated by urinary anaerobic infection caused by bacteroides vulgatus and peptococcus asaccharolyticus. | 1989 | 2489402 |
| [the detection of peptostreptococcus asaccharolyticus and bacillus cereus in clinical materials from a child who died of sepsis with a fulminant clinical course]. | a case of an acute disease with a rapid clinical course and a fatal outcome in the presence of irreversible toxicoinfectious shock, appearing in two children after the consumption of sheep kidneys, is described. the post mortem examination of the children revealed the presence of hemorrhagic, erosive and necrotic areas on the mucous membrane of the stomach, the duodenum and the upper section of the small intestine. from the material obtained by probing the stomach of one of the children 6 hours ... | 1996 | 9103069 |
| antibacterial activities of tosufloxacin against anaerobic bacteria and the electron micrograph of its bactericidal effects. | tosufloxacin, a quinolone, showed a broad antibacterial spectrum against gram-positive and gram-negative bacteria including anaerobic bacteria. tosufloxacin was 4- to 8-fold more active than levofloxacin and ciprofloxacin. the mic90 of tosufloxacin for clinical isolates of bacteroides fragilis, bacteroides vulgatus, bacteroides thetaiotaomicron and peptostreptococcus asaccharolyticus were 0.78, 0.39, 1.56 and 0.39 micrograms/ml, respectively. morphological observation with the scanning and trans ... | 1997 | 9142454 |
| amino acid sequence and molecular modelling of a thermostable two (4fe-4s) ferredoxin from the archaebacterium methanococcus thermolithotrophicus. | the amino acid sequence of a two (4fe-4s) ferredoxin from the methanogenic bacterium methanococcus thermolithotrophicus (fdmt) has been determined. this thermostable protein comprises 60 amino acid residues (mr 6541) and two (4fe-4s) clusters chelated to the protein through the eight cysteines. fdmt contains a relatively high number of lysines [5], threonines [4] and valines [10]. the three-dimensional molecular model generated from the peptococcus aerogenes x-ray structure keeps the characteris ... | 1991 | 1986796 |
| cloning and expression of the two genes coding for l-serine dehydratase from peptostreptococcus asaccharolyticus: relationship of the iron-sulfur protein to both l-serine dehydratases from escherichia coli. | the structural genes sdha and sdhb, coding for the alpha- and beta-subunits of the [4fe-4s] cluster containing l-serine dehydratase from peptostreptococcus asaccharolyticus, have been cloned and sequenced. expression of modified sdhb together with sdha in escherichia coli led to overproduction of active his6-tagged l-serine dehydratase. e. coli mew22, deficient in the l-serine dehydratase l-sd1, was complemented by this sdhba construct. the derived amino acid sequence of sdhba shares similaritie ... | 1997 | 9244285 |
| aminopeptidase activities in peptostreptococcus spp. are statistically correlated to gelatin hydrolysis. | one hundred peptostreptococcus isolates from five species were assessed for their ability to hydrolyze gelatin. most peptostreptococcus magnus (95.8%) and peptostreptococcus micros isolates (79.0%) hydrolyzed gelatin in contrast to peptostreptococcus asaccharolyticus (8.0%), peptostreptococcus anaerobius (10.0%), and peptostreptococcus prevotii isolates (16.7%). gelatin hydrolysis in peptostreptococcus magnus and peptostreptococcus micros isolates correlated (r = 0.80; p = 0.0019) with more amin ... | 1998 | 9606913 |
| partial purification of an iron-dependent l-serine dehydratase from clostridium sticklandii. | an oxygen-sensitive and highly unstable l-serine dehydratase was partially purified from the gram-positive anaerobe clostridium sticklandii. the final active preparation contained five proteins of 27, 30, 44.5, 46, and 58 kda as judged by sds-page. the n-terminal sequence of the 30 kda subunit showed some similarity to the alpha-subunits of the iron-containing l-serine dehydratases from clostridium propionicum and peptostreptococcus asaccharolyticus. oxygen-inactivated l-serine dehydratase from ... | 1998 | 9637015 |
| in vitro activity of gemifloxacin (sb 265805) against anaerobes. | gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ... | 1999 | 10471570 |
| activities of gemifloxacin (sb 265805, lb20304) compared to those of other oral antimicrobial agents against unusual anaerobes. | the activities of gemifloxacin (sb 265805, lb20304) and comparator agents were determined by an agar dilution method against 419 clinical strains of less-commonly identified species of anaerobes. gemifloxacin was generally more active than trovafloxacin against gram-positive strains by one to two dilutions. peptostreptococci (peptostreptococcus asaccharolyticus, peptostreptococcus magnus, peptostreptococcus micros, and peptostreptococcus prevotii) and porphyromonas spp. (porphyromonas asaccharol ... | 1999 | 10543754 |
| synergic activity, for anaerobes, of trovafloxacin with clindamycin or metronidazole: chequerboard and time-kill methods. | chequerboard titrations were used to test the activity of trovafloxacin, alone and in combination with clindamycin or metronidazole, against 156 gram-positive or gram-negative anaerobes, including 47 bacteroides fragilis group, 36 prevotella spp., 26 fusobacteria, 21 peptostreptococci and 26 clostridia. mic50/mic90 values (mg/l) of each drug alone against all 156 strains were: trovafloxacin, 0.5/1; clindamycin, 0.25/2; metronidazole, 1/2. synergy (fic indices </= 0.5) was seen in two strains wit ... | 2000 | 10797085 |
| induction of human immunodeficiency virus type 1 expression by anaerobes associated with bacterial vaginosis. | bacterial vaginosis (bv) is a common disorder characterized by increased levels of anaerobic bacteria in the genital tract. bv has been associated with an increased rate of sexual transmission of human immunodeficiency virus (hiv). the effects of bv-associated anaerobic bacteria on hiv expression in monocytoid cells and t cells were examined. peptostreptococcus asaccharolyticus and prevotella bivia stimulated hiv expression in monocytoid cells, whereas bacteroides ureolyticus, peptostreptococcus ... | 2000 | 10823756 |
| response of the central metabolism in corynebacterium glutamicum to the use of an nadh-dependent glutamate dehydrogenase. | the extensive use of 13c enrichments in precursor metabolites for flux quantification does not rely on nadph stoichiometries and can therefore be used to quantify reducing power fluxes. as an application of this concept, the nadph fluxes were quantified in an l-lysine producer of corynebacterium glutamicum grown into metabolic and isotopic steady state with [1-13c]glucose. in this case, where the organism's nadph-dependent glutamate dehydrogenase consumes reducing power, the nadph flux generated ... | 1999 | 10935753 |
| peptostreptococcus asaccharolyticus renal abscess: a rare cause of fever of unknown origin. | renal abscess is uncommon in pediatrics and is rarely a cause of fever of unknown origin. we recently cared for a patient who presented with a 3-week history of fever. an indium scan ultimately led to the diagnosis of a renal abscess. aspiration yielded peptostreptococcus asaccharolyticus. this unusual case prompted a review of the clinical and microbiologic features of renal abscess in pediatric patients at our hospital over the past 10 years. seven additional patients with a discharge diagnosi ... | 2001 | 11134475 |
| microbial analysis of bone collected during implant surgery: a clinical and laboratory study. | dental implant surgery produces bone debris which can be used to correct bone defects in the "simultaneous-augmentation" technique. however, this debris is potentially contaminated with oral bacteria. therefore, this study examined bone debris collected during dental implant surgery in order 1) to identify the microbial contaminants and 2) to compare the effects of two different aspiration protocols on the levels of microbial contamination. twenty-four partially dentate patients were randomly al ... | 2001 | 11251657 |
| proposal of the genera anaerococcus gen. nov., peptoniphilus gen. nov. and gallicola gen. nov. for members of the genus peptostreptococcus. | members of genus peptostreptococcus have previously been found to be distantly related to the type species, peptostreptococcus anaerobius, on the basis of 16s rdna sequence similarities. they were divided into three major phylogenetic groups, and their peptidoglycan structure and biochemical traits differed between groups. the reclassification of the species of these three groups into three new genera, peptoniphilus gen. nov., anaerococcus gen. nov. and gallicola gen. nov., is proposed. the genu ... | 2001 | 11491354 |
| azotobacter vinelandii ferredoxin i: a sequence and structure comparison approach to alteration of [4fe-4s]2+/+ reduction potential. | the reduction potential (e(0)') of the [4fe-4s](2+/+) cluster of azotobacter vinelandii ferredoxin i (avfdi) and related ferredoxins is approximately 200 mv more negative than the corresponding clusters of peptostreptococcus asaccharolyticus ferredoxin and related ferredoxins. previous studies have shown that these differences in e(0)' do not result from the presence or absence of negatively charged surface residues or in differences in the types of hydrophobic residues found close to the [4fe-4 ... | 2002 | 11704670 |
| [four cases of non-clostridial gas gangrene with diabetes mellitus]. | we report four cases of non-clostridial gas gangrene. all cases were associated with diabetes mellitus as the underlying disease. case 1: a 60-year-old male developed an ulcerative lesion on the dorsum of his left foot. peptostreptococcus asaccharolyticus, citrobacter freundii and staphyrococcus epidermidis were identified in culture from odoriferous pus. case 2: a 81-year-old female developed a lesion on her vulva spreading to the right lower abdomen. bacteroides bivius, peptostreptococcus asac ... | 2002 | 11915237 |
| [a comparative study of the various organisms causing salpingitis and their potential presence in iud wearers]. | a prospective study was undertaken to compare the enbdometrial and vaginal bacteria of a continuous series of 283 patients who had iuds removed between june 1, 1979 and june 1, 1983, by the same clinician. measures were taken to avoid contamination of the iud upon removal by the vaginal bacteria, but as a practical matter it was impossible to avoid all contact. the iuds involved were multiload copper 250 or 375, gyne t and t 200, nova t, and dimelys. the number of positive cultures from the ... | 1985 | 12339972 |
| antimicrobial properties of self-etching primer-bonding systems. | self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. the antibacterial effects of the self-etching primers clearfil se bond, mac bond, imperva fl bond, one-up bondf and prompt l-pop were evaluated using the bacteria streptococcus mutans atcc25175, peptostreptococcus anaerobius, peptostreptococcus prevotii, peptostreptococcus asaccharolyticus, lactobacillus acidophilus, la ... | 2003 | 12670069 |
| rapid identification of gram-positive anaerobic coccal species originally classified in the genus peptostreptococcus by multiplex pcr assays using genus- and species-specific primers. | here, a rapid and reliable two-step multiplex pcr assay for identifying 14 gram-positive anaerobic cocci (gpac) species originally classified in the genus peptostreptococcus (anaerococcus hydrogenalis, anaerococcus lactolyticus, anaerococcus octavius, anaerococcus prevotii, anaerococcus tetradius, anaerococcus vaginalis, finegoldia magna, micromonas micros, peptostreptococcus anaerobius, peptoniphilus asaccharolyticus, peptoniphilus harei, peptoniphilus indolicus, peptoniphilus ivorii and pepton ... | 2003 | 12855723 |
| [pathogenicity of staphylococcus asaccharolyticus distaso]. | | 1954 | 13171675 |
| bidirectional morphological changes induced by dexamethasone in morris hepatoma cell lines mca-rh7777 and mca-rh8994: independence of fibronectin and its receptor. | two morris hepatoma-derived cell lines, mca-rh7777 (7777) and mca-rh8994 (8994), exhibit different alterations in morphology upon exposure to glucocorticoid. after treatment with synthetic glucocorticoid dexamethasone (dex), 7777 cells show increased adhesiveness and more flattened shape, while dex-treated 8994 cells show decreased adhesiveness to substratum and exhibit a marked increase of round and detached cells. since fibronectin has been thought to play an important role in cell adhesivenes ... | 1991 | 1829041 |
| pharmacokinetics and pharmacodynamics of intravenous levofloxacin at 750 milligrams and various doses of metronidazole in healthy adult subjects. | the purpose of this investigation was to evaluate the steady-state pharmacokinetics, pharmacodynamics, and safety of intravenous levofloxacin at 750 mg administered once daily combined with three different dosages of intravenous metronidazole (500 mg every 8 h [q8h], 1,000 mg q24h, and 1,500 mg q24h). eighteen healthy adult subjects received all three combinations in a randomized, crossover fashion. serial blood and urine samples were collected on the third day of each study period. the 24-h are ... | 2004 | 15561831 |
| properties of the thermostable glutamate dehydrogenase of the mesophilic anaerobe peptostreptoccus asaccharolyticus purified by a novel method after over-expression in an escherichia coli host. | peptostreptococcus asaccharolyticus glutamate dehydrogenase (l-glutamate: nad+ oxidoreductase (deaminating); ec 1.4.1.2) overexpressed in escherichia coli has been purified by two new methods. enzyme made by the first method showed remarkable thermophilicity, with a temperature optimum of 60 degrees c, and also thermostability, which suggested the second, simpler method, incorporating a heat step. this produced 94 mg of homogeneous protein per litre culture medium. the basic kinetic parameters f ... | 2005 | 15727821 |
| [role of obligate anaerobes in infections in hospitalized patients and therapeutic options]. | monitoring of prevalence and antibiotic susceptibility of strictly anaerobic bacteria, causing infections in hospitalized patients, constitutes a part of a program for prudent use of antibiotics. the aim of the study was to assess prevalence of strictly anaerobic bacteria in patients hospitalized in a tertiary care hospital in 2001-2002 with reference to empiric antibiotic therapy. the most common gram-positive bacteria were clostridium difficile--27.7%, peptostreptococcus spp. and peptoniphilus ... | 2005 | 16134392 |
| infected pneumatocele following anaerobic pneumonia in adult. | we report a case of an infected pneumatocele in the course of anaerobic pneumonia in an adult. to the best of our knowledge, anaerobic pneumonia complicated by a pneumatocele in an adult has not previously been described. the pneumatocele occurred on the fifth day of hospitalization, and rapidly increased in size, with the development of a subsequent mixed anaerobe infection. a pig-tail catheter was inserted and the pus drained. the bacterial culture from the pus was positive for three anaerobes ... | 2005 | 16491835 |
| in vitro activity of oritavancin (ly333328), vancomycin, clindamycin, and metronidazole against clostridium perfringens, propionibacterium acnes, and anaerobic gram-positive cocci. | using an agar dilution method, we determined the in vitro activity of oritavancin, vancomycin, clindamycin and metronidazole against 114 unique clinical isolates of gram-positive anaerobes. mic(90)s (microg/ml) for oritavancin were as follows: clostridium perfringens 1.0, propionibacterium acnes 0.25, peptostreptococcus anaerobius 0.25, peptoniphilus asaccharolyticus 0.5, finegoldia magna 0.25, micromonas. micros 0.25, and anaerococcus prevotii 0.25. on a weight basis, oritavancin is slightly mo ... | 2005 | 16701537 |
| in vitro activities of dalbavancin and 12 other agents against 329 aerobic and anaerobic gram-positive isolates recovered from diabetic foot infections. | tests of dalbavancin's in vitro activity against 209 aerobic and 120 anaerobic isolates from pretreatment diabetic foot infections showed an mic(90) of < or =0.125 microg/ml against methicillin-susceptible staphylococcus aureus (mssa), methicillin-resistant s. aureus (mrsa), and 120 anaerobes (clostridium perfringens, other clostridia, peptoniphilus asaccharolyticus, finegoldia magna, and anaerococcus prevotii), compared to respective mic(90)s for mssa and mrsa of 0.5 and 1 microg/ml for vancomy ... | 2006 | 16870792 |
| in vitro activity of ceftobiprole against aerobic and anaerobic strains isolated from diabetic foot infections. | against 443 aerobic and anaerobic bacteria isolated from diabetic foot infections, ceftobiprole mics (microg/ml) at which 90% of the isolates tested were inhibited were as follows: methicillin-resistant staphylococcus aureus, 1; methicillin-susceptible s. aureus and staphylococcus lugdunensis, 0.5; anaerococcus prevotii, 0.125; finegoldia magna, 0.5; peptoniphilus asaccharolyticus, 1; peptostreptococcus anaerobius, 4; escherichia coli and enterobacter species, 0.125; klebsiella species, 2; and p ... | 2006 | 16982780 |
| activities of four frog skin-derived antimicrobial peptides (temporin-1dra, temporin-1va and the melittin-related peptides ar-23 and rv-23) against anaerobic bacteria. | the activities of two antimicrobial peptides belonging to the temporin family (temporin-1dra from rana draytonii and temporin-1va from rana virgatipes) and two peptides with structural similarity to the bee venom peptide melittin (ar-23 from rana tagoi and rv-23 from r. draytonii) were evaluated against a range of reference strains and clinical isolates of anaerobic bacteria. these peptides were selected because they show broad-spectrum growth inhibitory activity against reference strains of sev ... | 2007 | 17196372 |
| development of 16s rrna-based probes for the identification of gram-positive anaerobic cocci isolated from human clinical specimens. | fluorescent probes targeted at 16s rrna were designed for peptostreptococcus anaerobius and peptostreptococcus stomatis (pana134), parvimonas micra (pamic1435), finegoldia magna (fmag1250), peptoniphilus asaccharolyticus (pnasa1254), peptoniphilus ivorii (pnivo731), peptoniphilus harei (pnhar1466), anaerococcus vaginalis (avag1280) and anaerococcus lactolyticus (alac1438), based on the 16s rrna sequences of reference strains and 88 randomly chosen clinical isolates. these strains were also used ... | 2007 | 17714522 |
| probing the determinants of coenzyme specificity in peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis. | glutamate dehydrogenase (ec 1.4.1.2-4) from peptostreptococcus asaccharolyticus has a strong preference for nadh over nadph as a coenzyme, over 1000-fold in terms of kcat/km values. sequence alignments across the wider family of nad(p)-dependent dehydrogenases might suggest that this preference is mainly due to a negatively charged glutamate at position 243 (e243) in the adenine ribose-binding pocket. we have examined the possibility of altering coenzyme specificity of the peptostreptococcus enz ... | 2007 | 17850332 |
| quantitative survival of aerobic and anaerobic microorganisms in port-a-cul and copan transport systems. | transport media should preserve the viability and stability of microorganisms in clinical specimens. in this study, the port-a-cul transport system and the copan transport system without charcoal, both designed to preserve anaerobes, were evaluated. dacron swabs were inoculated with two combinations of facultative and anaerobic organisms typically found in vaginal swab samples. combination i contained candida albicans, escherichia coli, enterococcus spp., group b streptococci, lactobacillus cris ... | 2008 | 18579722 |
| piezoelectric polymer curvature sensor for measurement of regional curvature radius of lv wall. | to evaluate regional myocardial function, we developed a curvature sensor for direct and instantaneous measurement of the regional curvature radius of the lv wall. the sensor is a bimorph of two sheets of thin piezoelectric polymer film. the relation between output voltage of the sensor and the reciprocal of the known curvature radius has been shown to be linear. in anesthetized dogs, we inserted the curvature sensor into the subepimyocardium with a specially designed introducer. special care wa ... | 1988 | 3364584 |
| crystallization and preliminary structural analyses of glutamate dehydrogenase from peptoniphilus asaccharolyticus. | glutamate dehydrogenase (ec 1.4.1.2-4) from peptoniphilus asaccharolyticus has been expressed as a selenomethionine-derivatized recombinant protein and diffraction-quality crystals have been grown that are suitable for structure determination. preliminary structural analyses indicate that the protein assembles as a homohexameric enzyme complex in solution, similar to other bacterial and mammalian enzymes to which its sequence identity varies between 25 and 40%. the structure will provide insight ... | 2010 | 20445250 |
| effects of ru 486 on the mechanical properties of rabbit fallopian tubes. | | 1988 | 3377712 |
| structure of ferredoxin i from azotobacter vinelandii. | the structure of azotobacter vinelandii ferredoxin i (av fdi, 106 amino acids) has been redetermined, based on x-ray diffraction data from tetragonal crystals of the native protein and two heavy atom derivatives. the current model differs greatly from the one previously reported and is in agreement with arguments based on various spectroscopic and other methods. the unit cell parameters are a = b = 55.62 a and c = 95.51 a, whereas the space group was found to be p4(1)2(1)2 instead of p4(3)2(1)2. ... | 1988 | 3422475 |
| crystal structure of nad(+)-dependent peptoniphilus asaccharolyticus glutamate dehydrogenase reveals determinants of cofactor specificity. | glutamate dehydrogenases (ec 1.4.1.2-4) catalyse the oxidative deamination of l-glutamate to α-ketoglutarate using nad(p) as a cofactor. the bacterial enzymes are hexamers and each polypeptide consists of an n-terminal substrate-binding (domain i) followed by a c-terminal cofactor-binding segment (domain ii). the reaction takes place at the junction of the two domains, which move as rigid bodies and are presumed to narrow the cleft during catalysis. distinct signature sequences in the nucleotide ... | 2011 | 22068154 |
| deficiency of eosinophil peroxidase detected by automated cytochemistry. | | 2011 | 3122494 |
| the protein responsible for center a/b in spinach photosystem i: isolation with iron-sulfur cluster(s) and complete sequence analysis. | the 9 kda polypeptide from spinach photosystem i (ps i) complex was isolated with iron-sulfur cluster(s) by an n-butanol extraction procedure under anaerobic conditions. the polypeptide was soluble in a saline solution and contained non-heme irons and inorganic sulfides. the absorption spectrum of this iron-sulfur protein was very similar to those of bacterial-type ferredoxins. the amino acid sequence of the polypeptide was determined by using a combination of gas-phase sequencer and conventiona ... | 1988 | 3049567 |
| lactic acid-utilizing bacteria in ruminal fluid of a steer adapted from hay feeding to a high-grain ration. | a mature, rumen-cannulated steer fed coastal bermuda grass hay for 8 weeks was adapted to a high-grain ration by stepwise increases in grain over a period of 4 weeks. the grain rations had concentrate-to-roughage ratios of 40:60, 70:30, and 85:15 and were fed for 10, 7, and 11 days, respectively. numerical estimates of lactate-utilizing bacteria in ruminal fluid of the steer the last 3 days each ration was fed were made by colony counts. lactate-utilizing bacteria were identified as megasphaera ... | 1976 | 1275348 |
| accomplishments of a salaried blood donor recruiter in a municipal hospital. | | 2015 | 4411811 |
| consecutive infected aneurysms caused by salmonella. | treatment of infected aneurysms consists of operation, which depends on the location and pathologic type, and antibiotic therapy, before and at least six weeks following operation. the authors present a case of two consecutive aneurysms occurring in a patient with salmonella infection. full recovery was obtained after surgical excision of the aneurysms, prolonged antibiotic therapy, and cholecystectomy. | 1988 | 3067740 |
| a new expression system for mammalian cells based on putative replicator sequences of the mouse and a truncated thymidine kinase gene. | we have constructed a new expression vector for mammalian cells. the vector contains a truncated tk gene for amplification under selective conditions, a sequence putatively supporting the replication of plasmid dna in eukaryotic cells (murine autonomously replicating sequence) and an expression cassette for the cdna to be studied. as a model cdna we have used that of human tissue-type plasminogen activator (t-pa). analysis of hirt supernatants and chromosomal dna from l cells, prepared six weeks ... | 1988 | 3149608 |
| effect on bone of withdrawal of oestrogen therapy. | | 1999 | 89300 |
| poison ivy (rhus) dermatitis. | | 2001 | 150451 |
| chromium: a review of environmental and occupational toxicology. | the following topics are covered in this brief review on the environmental and occupational toxicology of chromium: occurrence, production and uses of chromium and chromium compounds; experimental toxicology; chromium toxicity for man; hygienic and ecologic aspects of chromium contamination of the environment. the review provides a conclusive evidence which suggests that chromium, especially its hexavalent form, is both toxic and carcinogenic, but its trivalent form is physiologically essential ... | 1985 | 3157743 |
| in the aftermath: health care in kuwait city. | | 2007 | 1865865 |
| chloramphenicol and tetracycline usage in outpatient practice. | | 1998 | 121377 |
| deaths of infants after triple vaccine. | | 1999 | 89408 |
| tertiary structure of bacillus thermoproteolyticus [4fe-4s] ferredoxin. evolutionary implications for bacterial ferredoxins. | the structure of a low-potential [4fe-4s] ferredoxin from bacillus thermoproteolyticus has been solved using anomalous scattering data from iron atoms in the diffraction data of native crystals and refined partially to a crystallographic r-factor of 0.33, with 2.3 a (1 a = 0.1 nm) resolution data. the least-squares refinement based on the bijvoet differences has determined that the four iron atoms in the cluster are an equal distance, approximately 2.8 a, apart. the nh ... s hydrogen bonds betwe ... | 1988 | 3351918 |
| metabolism of histidine by peptococcus aerogenes. | | 1967 | 6035532 |
| the vaginal microbial flora in non-specific vaginitis. | the facultative and strictly anaerobic vaginal microbial flora was investigated in 40 women with non-specific vaginitis and in 40 control women seen in private gynaecological practice. gardnerella vaginalis, anaerobic gram-negative bacilli, anaerobic gram-negative and gram-positive cocci were all associated with non-specific vaginitis (p less than 0.001), whereas lactobacilli occurred less frequently in non-specific vaginitis than in controls (p less than 0.01). the most common anaerobes were ve ... | 1982 | 6985215 |
| aerobic and anaerobic bacterial flora of burns in children. | aerobic and anaerobic bacterial flora of burn sites in 180 children were monitored. specimens were obtained twice a week; each patient had between one and 21 cultures taken (mean 2.4). a total of 392 specimens were collected over 2 years, of which 319 were positive. aerobic bacteria alone were present in 225 specimens (71%) and anaerobic bacteria alone were present in 26 (8%). mixed aerobic and anaerobic bacteria were present in 68 burn specimens (21%). a total of 580 isolates (448 aerobes and 1 ... | 1981 | 7218398 |
| lesser omental thickness in normal children: baseline for detection of portal hypertension. | the lesser omentum between the left lobe of the liver and the aorta at the level of the celiac axis harbors the left gastric vein and artery, lymphatics, nerves, and some fatty tissue. in one study, it was found to be thickened in most of 50 children with portal hypertension, but normal values were not established. lesser omental thickness was measured in 150 children without systemic, liver, or renal disease, and it was found that it did not exceed 1.7 times the diameter of the aorta at the sam ... | 2014 | 3898783 |
| crystal structure of the ferredoxin i from desulfovibrio africanus at 2.3 a resolution. | the crystal structure of the ferredoxin i from the sulfate-reducing bacterium desulfovibrio africanus (dafdi) has been solved and refined by x-ray diffraction. the crystals are orthorhombic with a = 96.6 a, b = 58.1 a, and c = 20.7 a, space group p2(1)2(1)2, and two ferredoxin molecules per asymmetric unit. the initial electron density map has been obtained by combining phasing by molecular replacement methods, anomalous scattering, and noncrystallographic averaging. the final crystallographic r ... | 1994 | 7803404 |
| calculation of the redox potentials of iron-sulfur proteins: the 2-/3-couple of [fe4s*4cys4] clusters in peptococcus aerogenes ferredoxin, azotobacter vinelandii ferredoxin i, and chromatium vinosum high-potential iron protein. | calculations of the redox potentials of the 2-/3-couples of [fe4s*4cys4] clusters in the iron-sulfur proteins peptococcus aerogenes ferredoxin (pafd), azotobacter vinelandii ferredoxin i (avfdi) and chromatium vinosum high potential iron protein (cvhipip) based on the protein dipoles langevin dipoles (pdld) method are reported. the structures of these proteins have been determined by x-ray crystallography; in the case of pafd the structure has recently been revised due to a change in the sequenc ... | 1994 | 8086408 |
| azotobacter vinelandii ferredoxin i. alteration of individual surface charges and the [4fe-4s]2+/+ cluster reduction potential. | the structures of azotobacter vinelandii ferredoxin i (avfdi) and peptococcus aerogenes ferredoxin (pafd), near their analogous [4e-4s]2+/+ clusters, are highly conserved (backes, g., mino, y., loehr, t.m., meyer, t.e., cusanovich, m.a., sweeney, w.v., adman, e.t., and sanders-loehr, j. (1991) j. am. chem. soc. 11, 2055-2064). despite these similarities, the reduction potential (e0') of the avfdi [4fe-4s]2+/+ cluster is more than 200 mv more negative than that of pafd. we have tested the contrib ... | 1994 | 8132582 |
| [combined treatment of rheumatological patients at a health resort]. | | 2003 | 154775 |
| [nuclear surface of buccal cells as a function of the nature of the stain. measurements with an image analyzing computer]. | measure of nuclear areas was performed on buccal smears by the quantimet 720 image analysing computer. five different staining procedures were used and we consider that toluidin blue at ph 4 is the best for a fine quantitative caryometry. | 2001 | 88973 |
| the relationship of the pulp and periodontium. | | 2003 | 149297 |
| [an epidemiology of hypertension in japanese]. | | 2006 | 1635191 |
| site-specific mutagenesis of rhodobacter capsulatus ferredoxin i, fdxn, that functions in nitrogen fixation. role of extra residues. | one of the two [4fe-4s]-type clusters of the rhodobacter capsulatus ferredoxin i, fdxn, was modified through site-specific mutagenesis of the distinctive features of the second cluster-binding motif, cys38-x2-cys41-x8-cys50-x3-cys54-x4-cys59. first, various mutagenized products were tested to learn whether they could rescue the decreased capacity of an fdxn-null strain msa1 to fix nitrogen: the phenotype of msa1 was reassessed to nifs (slow growth by nitrogen fixation) from our previous descript ... | 1996 | 8940149 |
| y13c azotobacter vinelandii ferredoxin i. a designed [fe-s] ligand motif contains a cysteine persulfide. | ferredoxins that contain [4fe-4s]2+/+ clusters often obtain three of their four cysteine ligands from a highly conserved cysxxcysxxcys sequence motif. little is known about the in vivo assembly of these clusters and the role that this sequence motif plays in that process. in this study, we have used structure as a guide in attempts to direct the formation of a [4fe-4s]2+/+ in the [3fe-4s]+/0 location of native (7fe) azotobacter vinelandii ferredoxin i (avfdi) by providing the correct three-dimen ... | 1997 | 9188450 |
| structure at ph 6.5 of ferredoxin i from azotobacter vinelandii at 2.3 a resolution. | ferredoxin i from azotobacter vinelandii (avfdi) is an iron-sulfur protein composed of 106 amino acids, seven fe atoms and eight inorganic s* atoms. a crystallographic redetermination of its structure showed the originally reported structure to be incorrect. we report here the crystal structure of avfdi at ph 6.5. extensive refinement has led to a final r value of 0.170 for all 6986 non-extinct reflections in the range 10-2.3 a using a solvent model which includes 98 discrete solvent atoms with ... | 1993 | 15299532 |
| anaerosphaera aminiphila gen. nov., sp. nov., a glutamate-degrading, gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste. | a strictly anaerobic, mesophilic and aminolytic strain (wn036(t)) was isolated from a methanogenic reactor treating waste from cattle farms. cells were gram-positive cocci, often occurred in pairs and were non-motile. although spore formation was not confirmed by microscopic observation of cells, the strain produced thermotolerant cells. the optimum temperature for growth was 35-37 degrees c and the optimum ph was 6.7. oxidase, catalase and nitrate-reducing activities were negative. the novel st ... | 2009 | 19643871 |
| body mass index variations: centiles from birth to 87 years. | this report provides body mass index (weight/height2) values for the french population from birth to the age of 87 years. bmi curves increase during the first year, decrease until the age of 6, increase again up to 65 years and decrease thereafter. these variations reflect the total changes of fat body mass during life. the 50th centile values of wt/ht2 at the ages of 20, 40, 60, 80 years are 21.5, 24.6, 25.4, 24.4 kg/m2 for men and 20.6, 22.6, 24.1, 23.4 kg/m2 for women. the values for the 3rd, ... | 2007 | 1855495 |
| mistaken identity of peptoniphilus asaccharolyticus. | | 2011 | 21248090 |
| [analysis of the nursing factors responsible for expanding the sphere of daily activities of patients with parkinson's disease]. | | 2008 | 2612141 |
| increased formation of dna adducts in cultured fibroblasts of patients with aplastic anemia after in vitro incubation with benzo(a)pyrene. | in inbred mice strain dba/2, genetically controlled differences in aryl hydrocarbon hydroxylase (ahh) activity have been demonstrated that predispose to aplastic anemia. to test the hypothesis of a similar mechanism in humans, we studied the formation of benzo(a)pyrene dna adducts and water-soluble metabolites in skin fibroblasts from eight patients with aplastic anemia and eight normal controls. the ratio of water-soluble metabolites and dna adducts was 46.5 +/- 16.6 in patients and was signifi ... | 2008 | 2289825 |
| anti-anaerobic activity of serum from patients treated with tigecycline for skin/soft tissue infections. | to gain additional data concerning the anti-anaerobic activity of tigecycline in serum, we analyzed blood samples from six patients with a complicated skin/soft tissue infection who were receiving iv tigecycline 50 mg every 12 h. venous blood samples were obtained after multiple doses of tigecycline at 1, 6 and 12 h after the initiation of a 1 h iv infusion. sera from these samples were tested to determine serum inhibitory and bactericidal activity over time against 4 anaerobic bacteria (bactero ... | 2011 | 21376823 |
| [iatrogenic radiation sickness]. | | 2015 | 4512267 |
| spinal cord serotonin release and raised blood pressure after brainstem kainic acid injection. | the recently developed technique of in vivo dialysis has permitted us to make direct measurements of serotonin release in a specific region of the spinal cord and to relate this to changes in blood pressure elicited by chemical stimulation of the brainstem. in the present experiments we have shown that chemical stimulation of bulbospinal neurons in the region of the b3 cell group in the ventromedial medulla, causes an increase in the release of serotonin in the thoracic spinal cord and that this ... | 1986 | 3697690 |
| toxicity of perfluorinated fatty acids for human and murine b cell lines. | the toxicity of two perfluorinated fatty acids, penta decafluoro-n-octanoic acid (pfoa) and nonadecafluoro-n-decanoic acid (ndfda), on three mammalian b cell lines was evaluated. cells were exposed to the perfluorinated molecules for either 24 or 48 hr under a variety of culture conditions. immunoglobulin secretion and surface membrane expression were unaffected by both pfoa and ndfda at sublethal concentrations. lethal effects of pfoa and ndfda are diminished by either lowering culture temperat ... | 1986 | 3764929 |
| fatal leishmaniasis in renal-transport patient. | | 1999 | 88649 |
| os calcis osteotomy in the management of deformities of the hindfoot in spinal dysraphism. | the results of 52 primary os calcis osteotomies in 44 patients with spinal dysraphism are reviewed. there were 42 varus hindfoot deformities and ten valgus hindfoot deformities. the results indicate that the operation is simple and uncomplicated, but over a mean 5.8 year follow-up for the varus feet and 6.5 years for the valgus feet, over 50% of the feet developed either a moderate or severe recurrence of deformity. os calcis osteotomy appeared from this series to be a moderately successful inte ... | 2013 | 3811619 |
| conception in a phenylketonuric woman. | | 1999 | 84971 |
| [reconstructive plastic surgery of the humeral condyles following removal of endoprostheses of the elbow versus arthrodesis]. | the condyles of the elbow are of utmost importance for the stability of this joint. this is particularly evident in cases where elbow prostheses had to be removed because of loosening or infection and where the condyles had been deliberately resected at the primary operation. in order to obtain a functional elbow with sufficient stability and mobility after removal of the prostheses, we can reconstruct the condyles by means of a large autologous bone graft, which we take from the midpart of the ... | 2013 | 3658417 |
| [clinical value of the time of formation of extrasystoles]. | | 2014 | 4187742 |
| reactivity of lymphoid cells from mice humorally tolerant to histocompatibility antigens. | | 2014 | 4148456 |
| [ovarian vein syndrome]. | | 2015 | 4704157 |
| [editorial: life span and life quality]. | | 2015 | 4846704 |
| [editorial: life span and life quality]. | | 2015 | 4846704 |
| insensible water loss. the inter-subject variation related to skin temperature, forearm circumference and sweat gland activity. | | 2015 | 4665981 |