| metabolic zonation in liver of diabetic rats. zonal distribution of phosphoenolpyruvate carboxykinase, pyruvate kinase, glucose-6-phosphatase and succinate dehydrogenase. | the activities and zonal distribution of key enzymes of carbohydrate metabolism were studied in livers of diabetic rats. 48 h after alloxan treatment the following alterations were observed, intermediate values being reached after 24 h: blood glucose, acetoacetate and beta-hydroxybutyrate were increased to more than 500%; liver glycogen was reduced to about 10%. portal vein insulin was reduced to below 10%, portal glucagon was increased to almost 200%. the glucogenic enzymes phosphoenolpyruvate ... | 1985 | 2988584 |
| regulation of the glucose phosphotransferase system in brochothrix thermosphacta by membrane energization. | uptake of 2-deoxyglucose, alpha-methylglucopyranoside, and glucose into intact cells of brochothrix thermosphacta (formerly microbacterium thermosphactum, atcc 11509) was stimulated by kcn or cccp. the glucose analogs were recovered almost totally as the sugar phosphates. membrane vesicles were isolated from protoplasts and shown to be right side out by freeze fracturing and by using atpase as a marker for the cytoplasmic membrane surface. uptake of glucose into vesicles was dependent on the pre ... | 1985 | 2995314 |
| the affinity of (-)-propranolol for beta 1- and beta 2-adrenoceptors of human heart. differential antagonism of the positive inotropic effects and adenylate cyclase stimulation by (-)-noradrenaline and (-)-adrenaline. | an appraisal of the affinity of (-)-propranolol was made for beta-adrenoceptors of isolated heart preparations and myocardial membrane particles from patients undergoing open heart surgery. in order to eliminate possible distorting influences of neuronal and extraneuronal uptakes of catecholamines on the affinity estimates for (-)-propranolol, isolated tissues were pretreated once with 5 or 10 mumol/l phenoxybenzamine for 2 h. phenoxybenzamine caused potentiation of the positive inotropic effect ... | 1985 | 2999616 |
| isolation and buoyant density of pneumocystis carinii in percoll gradients. | a new gradient material, percoll, a collodial suspension of silica particles covered with polyvinylpyrolidon, has been used to isolate pneumocystis carinii from infected rat and human lung tissues. the specific gravity of pneumocystis carinii was shown to be within the range of 1.018-1.062 g/ml with most pneumocysts within 1.033-1.049 g/ml. | 1985 | 3001637 |
| on the spin trapping and esr detection of oxygen-derived radicals generated inside cells. | recently several attempts to identify oxygen-derived radicals in whole cells by spin trapping and electron spin resonance have been reported by using 5,5-dimethyl-1-pyrroline-n-oxide as the spin trap. in the present study, the feasibility of this method is examined. chinese hamster v79 cells and human erythrocytes served as the test systems, while oh radicals were generated by gamma radiolysis. several spin traps were used to scavange the radicals and a distinction between exo- and endocellular ... | 1986 | 3020550 |
| on the spin trapping and esr detection of oxygen-derived radicals generated inside cells. | recently several attempts to identify oxygen-derived radicals in whole cells by spin trapping and electron spin resonance have been reported by using 5,5-dimethyl-1-pyrroline-n-oxide as the spin trap. in the present study, the feasibility of this method is examined. chinese hamster v79 cells and human erythrocytes served as the test systems, while oh radicals were generated by gamma radiolysis. several spin traps were used to scavange the radicals and a distinction between exo- and endocellular ... | 1986 | 3020550 |
| regulation of enzymes by ligand induced change in polymerization. | | 1986 | 3020906 |
| mapping of the gene for anti-müllerian hormone to the short arm of human chromosome 19. | the gene coding for human anti-müllerian hormone (amh) was localized to subbands p13.2----p13.3 on chromosome 19, using in situ hybridization and southern blot analysis of a panel of man-mouse and man-hamster somatic cell hybrids. | 1987 | 3028714 |
| structure-activity relationships for steroid interaction with the gamma-aminobutyric acida receptor complex. | certain steroids are potent barbiturate-like modulators of the gamma-aminobutyric acida (gaba) receptor-chloride ionophore complex in rat brain membranes. at nanomolar to low micromolar concentrations, these steroids stimulate [3h]flunitrazepam and [3h] muscimol binding and displace the convulsant [35s]t-butylbicyclophosphorothionate from its binding site in an allosteric manner, in addition to enhancing cl- conductance responses to gaba recorded in cultured rat hippocampal and spinal neurons. a ... | 1987 | 3033209 |
| structure-activity relationships for steroid interaction with the gamma-aminobutyric acida receptor complex. | certain steroids are potent barbiturate-like modulators of the gamma-aminobutyric acida (gaba) receptor-chloride ionophore complex in rat brain membranes. at nanomolar to low micromolar concentrations, these steroids stimulate [3h]flunitrazepam and [3h] muscimol binding and displace the convulsant [35s]t-butylbicyclophosphorothionate from its binding site in an allosteric manner, in addition to enhancing cl- conductance responses to gaba recorded in cultured rat hippocampal and spinal neurons. a ... | 1987 | 3033209 |
| coxsackie b myocarditis. | | 1987 | 3040137 |
| the function of superoxide dismutase during the enzymatic formation of the free radical of ribonucleotide reductase. | an enzyme system from escherichia coli activates an inactive form of ribonucleotide reductase by transforming a tyrosine residue of the enzyme into a cationic free radical. the process requires nad(p)h, a flavin, dithiothreitol, and oxygen and at least three proteins. after purification to near homogeneity two of the proteins were identified as superoxide dismutase and nad(p)h:flavin oxidoreductase (fontecave, m., eliasson, r., and reichard, p. (1987) j. biol. chem. 262, 12325-12331). the nature ... | 1987 | 3040738 |
| histologic evaluation of response to implantation of polyvinilidine plates in dogs and rats. | the effect of implantation of polyvinilidine spinal plates was studied when placed on spinous processes of 11 mixed breed dogs and when placed within the thoracolumbar musculature of 12 male white rats. specimens were also obtained from soft tissues adjacent to polyvinilidine plates 2 to 3 months after clinical surgery to repair spinal subluxations in three dogs. a similar pattern of dense, organized fibrous tissue interspersed with focal congregations of lymphocytes, plasma cells, and multinucl ... | 1988 | 3067442 |
| glycosaminoglycans in normal and atherosclerotic human coronary arteries. | glycosaminoglycans (gags) were studied in normal and atherosclerotic coronary arteries of 15- to 60-year-old finnish men who had died accidentally. the gags were fractionated and quantified with electrophoretic techniques. the contents of sulfated gags (micrograms/cm2 vessel surface area) increased continuously until 20 to 30% of the vessel surface area was covered with fibrous plaques, after which they started to decrease. the largest increases were seen in chondroitin sulfates a and c and derm ... | 1986 | 3083156 |
| [primary leiomyosarcoma of the pulmonary artery. apropos of a case]. | a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ... | 1987 | 3122693 |
| follicle-stimulating hormone induces deoxyribonucleic acid polymerase alpha before deoxyribonucleic acid synthesis in immature rat ovarian follicles perfused in vitro. | to examine the effects of follicle-stimulating hormone (fsh) on cell proliferation and cell division in ovaries, deoxyribonucleic acid (dna) polymerase activities and dna concentrations were determined in ovarian follicles of immature, hypophysectomized, estradiol-treated rats perfused in vitro. ovine fsh (2-20 micrograms/ml) significantly enhanced the dna polymerase alpha activity in the follicles perfused for 180-300 minutes, whereas the dna polymerase beta activity and dna concentration showe ... | 1988 | 3126468 |
| antibacterial activity of plantaricin sik-83, a bacteriocin produced by lactobacillus plantarum. | lactobacillus plantarum sik-83 produces a bacteriocin, designated plantaricin sik-83, which inhibits 66 of 68 lactic acid bacteria from the genera lactobacillus, leuconostoc, pediococcus and streptococcus. a 500-fold dilution of l. plantarum sik-83 mrs culture supernatant with phosphate buffer was sufficient to kill 10(5) cells/ml of pediococcus pentosaceus within 120 s. the killing of a sensitive population followed exponential kinetics. it was shown that the bacteriocin binds specifically to s ... | 1988 | 3139056 |
| an electron microscope study of the development of the exocrine and endocrine pancreas with special reference to intercellular junctions. | intercellular junctions in pancreatic acinar, duct and endocrine cells were studied by thin section and freeze-fracture methods in developing rats and mice. undifferentiated cells were joined by the zonula occludens and isolated fragments of tight junctional strands. small gap junctions were either occasionally associated with tight junctional strands or appeared independent of them. during the morphological differentiation of acinar cells, strands of the zonula occludens developed to form a com ... | 1988 | 3147689 |
| prolonged expression of differentiated phenotype by chondrocytes cultured at low density on a composite substrate of collagen and agarose that restricts cell spreading. | the dedifferentiation of chondrocytes in culture is frequently associated with transition from a rounded to a spread morphology. a number of culture methods which prevent cell spreading have been described; however, all have disadvantages that limit their widespread use. in this paper we describe a new technique which allows prolonged cultivation of attached chondrocytes at low density while inhibiting spreading: the cells are grown on a composite substrate of agarose and collagen. by varying th ... | 1988 | 3209004 |
| fine structure of the a-band in cryo-sections. diversity of m-band structure in chicken breast muscle. | electron micrographs of longitudinal ultrathin cryo-sections and plastic sections of chicken pectoralis muscle together with their average images have been used to study in detail the axial structure of the m-band. it was found that m-band structure could vary markedly in different fibres, even within the white part of the muscle. strong m-band density ("m-bridges") could be seen at m4 and m4' in all fibres. on the other hand the density at m1 or m6 could vary systematically. some fibres (probab ... | 1988 | 3209858 |
| the cell wall structure of a magnesium-dependent halobacterium, halobacterium volcanii cd-2, from the dead sea. | cell wall preparations from the magnesium-dependent halophilic bacterium, halobacterium volcanii, were studied by high-resolution electron microscopy complemented with image analysis and processing. for ultrastructural studies, specimens were prepared by a variety of methods, including negative staining, and metal shadowing after air-drying, freeze-drying, or freeze-fracturing and etching. all methods revealed the cell wall to be composed of a near-hexagonal lattice of unit cells having a center ... | 1988 | 3209861 |
| functionally polarized layers formed by epidermal cells on a permeable transparent collagen film. | rat epidermal cells were cultured on a transparent collagen film, which was permeable to low mr substances. then the cells were bathed in both media (apical and basal), which were separated by the collagen membrane. the cells formed a multi-layered epidermal sheet with well-developed structures of desmosomes. this sheet on a permeable support was found to be an effective permeability barrier for glucose and amino acids. the epidermal layer showed functional polarity for the uptake and excretion ... | 1988 | 3255753 |
| anti-idiotypic antibody used for the localization of parenterally administered monoclonal anti-progesterone antibody in mice. | affinity-purified rabbit and sheep anti-idiotypic antisera raised against mouse monoclonal anti-progesterone igg1 antibody (db3) or mouse myeloma igg1 protein p3 (mopc 21) showed high binding specificities to the respective idiotypes used for immunization as determined by ria or elisa. they have been used in an indirect immunofluorescent method to demonstrate the localization of parenterally administered idiotypes in pregnant or pseudopregnant balb/c mouse frozen tissue preparations, at known st ... | 1988 | 3264081 |
| in human monocytes a strong correlation exists between expression of the m3 antigen, fc-mediated phagocytic activity and failure to participate in extracellular antibody-dependent cytotoxicity. | human fresh blood monocytes can phagocytize and lyse antibody-coated target cells by contact with membrane fc receptors. recently, the monocyte differentiation antigen leu m3 has been described to be associated with monocyte/macrophage maturation pathway and to be linked to functionally distinct monocyte subsets. in the present study peripheral blood monocytes were separated into m3+ and m3- subsets, and evaluated for their ability to mediate antibody-driven effector functions. a clear cut funct ... | 1988 | 3281851 |
| role of host immune system in bb/wor rat. predisposition to diabetes resides in bone marrow. | the role of the immune system in the development of autoimmune diabetes mellitus in the bb/wor rat was studied with bone marrow transplantation methodology. in the first experiment, diabetes-prone (dp) and diabetes-resistant (dr) bb/wor rats were irradiated and reconstituted with bone marrow to create both reciprocal (dp donor----dr host; dr donor----dp host) and syngeneic (dr----dr; dp----dp) histocompatible chimeras. both susceptibility and resistance to subsequent spontaneous diabetes in thes ... | 1988 | 3282942 |
| humoral response in mice immunized with outer membrane proteins of shigella flexneri. | intraperitoneal immunization of mice with outer membrane proteins (omp) of sh. flexneri induced in the animals a synthesis of specific antibodies. their level determined by elisa test was found to be relatively low in the sera of animals immunized with a single dose (10 micrograms) of omp; it was markedly higher in mice immunized with two doses of omp, and very high after three fold immunization. the specific antibodies maintained in the animals for 8-16 weeks after immunization. anti-omp sera g ... | 1986 | 3296993 |
| drug permeability of the endolymphatic sac. | intravenously injected kanamycin sulfate (km) was located in the endolymphatic sac (es) of guinea pigs by an immunohistologic method. kanamycin sulfate had passed through the capillaries in the subepithelial layer and reached the epithelial layer as early as 1 minute after injection. the amount of km in the epithelial layer gradually increased with time (until 30 minutes after injection). the km also accumulated in floating cells in the es. these results tend to indicate that the es is rather ea ... | 1987 | 3304088 |
| nitrogenase of klebsiella pneumoniae. kinetic studies on the fe protein involving reduction by sodium dithionite, the binding of mgadp and a conformation change that alters the reactivity of the 4fe-4s centre. | the kinetics of reduction of indigocarmine-dye-oxidized fe protein of nitrogenase from klebsiella pneumoniae (kp2ox) by sodium dithionite in the presence and absence of mgadp were studied by stopped-flow spectrophotometry at 23 degrees c and at ph 7.4. highly co-operative binding of 2mgadp (composite k greater than 4 x 10(10) m-2) to kp2ox induced a rapid conformation change which caused the redox-active 4fe-4s centre to be reduced by so2-.(formed by the predissociation of dithionite ion) with k ... | 1987 | 3318808 |
| an in-vivo model to examine the electromyographic activity of isolated myometrial tissue from pregnant sheep. | strips (2.5 x 3.5 cm) of myometrium alone (myo) or endometrium/myometrium (endo/myo) were removed from the pregnant horn of sheep (day 110 of gestation) and transplanted to sites within the omental fat. these explants developed regular bursts of electromyographic (emg) activity over a period of 7-10 days, as well as a dose-dependent stimulatory response to oxytocin (50-200 mu i.v.). the frequency (per 2 h) of emg bursts in the myo (5.3 +/- 0.2) and endo/myo (5.2 +/- 0.3) explants was significant ... | 1988 | 3339597 |
| a legal framework to eradicate female circumcision. | | 1988 | 3386457 |
| a legal framework to eradicate female circumcision. | | 1988 | 3386457 |
| estrogen receptors in cultured rabbit articular chondrocytes: influence of age. | primary cultures of pubertal and prepubertal rabbit articular cartilage cells were performed. total homogenates or cell extracts were used to determine the specific binding of 17 beta-estradiol. a comparative study was undertaken with tissue minces homogenized without enzymatic treatment. scatchard analysis of cell or tissue extracts revealed the presence of a high-affinity receptor with kd values of 0.55 +/- 0.16 nm and 0.12 +/- 0.03 nm in prepubertal and pubertal rabbit chondrocytes respective ... | 1988 | 3419165 |
| an automated perifusion system for the study of rat spermatogenesis in vitro. | we report details of an automated perifusion system for the study of spermatogenesis in vitro. a potential of this new system is the evaluation of hormone/growth factor effects on the mitotic clonal expansion of spermatogonia and the meiotic and postmeiotic clonal differentiation of spermatogenic cells in coculture with sertoli cells and in incubated seminiferous tubular segments. | 1987 | 3445968 |
| immunological studies on crohn's disease. iv. decreased adcc activity. | antibody dependent cell-mediated cytotoxicity (adcc) activity was studied in 16 japanese patients with crohn's disease (cd) and 16 healthy controls. adcc activity was assessed by measuring 51cr release from antibody coated human b-cell line. significantly (p less than 0.002) depressed adcc activity was found in patients with cd, although adcc activity was not directly related to the disease activity. to determine causative factors related to reductions in adcc activity, circulating immune comple ... | 1986 | 3485720 |
| activity of a partially purified human bcgf on murine assays for b-cell stimulatory factors. i. bcgf ii-like activity of human bcgf. | to further characterize a human b-cell growth factor (bcgf) produced by phytohemagglutinin (pha) p-stimulated peripheral blood t cells, a partially purified preparation of this material was tested in a number of murine assays for b-cell stimulatory factors (bsf). human bcgf lacked murine bsf-1 activity as assessed via the induction of polyclonal proliferation of anti-igm-stimulated murine b cells; however, this material consistently augmented the proliferative response of murine b cells to anti- ... | 1987 | 3499989 |
| immunocytochemical localization of cathepsin b in rat kidney. ii. electron microscopic study using the protein a-gold technique. | thin sections of lowicryl k4m-embedded materials were labeled with protein a-gold complex. gold particles representing the antigen sites for cathepsin b were exclusively confined to lysosomes of each segment of the nephron. the heaviest labeling was noted in the lysosomes of the s1 segment of the proximal tubules. labeling intensity varied considerably with the individual lysosomes. lysosomes of the other tubular segments, such as the s2 and s3 segments of the proximal tubules, distal convoluted ... | 1986 | 3519753 |
| the carrier state: group b streptococcus. | | 1986 | 3528112 |
| antimicrobial activity of ro 19-5247 (t-2525), a new oral cephalosporin, tested against 7,745 recent clinical isolates. | the susceptibility testing of 7,745 recent clinical isolates from four medical centers showed ro 19-5247 to be eight- to greater than 64-fold more active than cephalexin against the enterobacteriaceae. ro 19-5247 was comparable with cephalexin in anti-staphylococcal activity (mic50, 4.0 micrograms/ml) and fourfold more active than cefixime. none of the oral cephalosporins were effective (mic50, greater than 32 micrograms/ml) against enterococci, pseudomonas aeruginosa and p. maltophilia. beta-la ... | 1987 | 3568594 |
| nacl and ca delivery at the bend of rat deep nephrons decreases during antidiuresis. | the effects of 1-desamino-8-d-arginine vasopressin (ddavp) on superficial and juxtamedullary nephrons were investigated by micropuncture in diabetes insipidus (di) brattleboro rats chronically treated with the peptide. the rats, acutely deprived of endogenous calcitonin, parathyroid hormone (pth), and glucagon [hormone-deprived (hd) rats], were examined either 4 days after cessation of ddavp treatment (hdt, control diuretic rats) or when the treatment was continued until the micropuncture experi ... | 1987 | 3591953 |
| effect of metoclopramide on adrenal secretion in sheep: influence of dexamethasone and sodium intake. | metoclopramide, a competitive dopamine antagonist, stimulates aldosterone in man and monkey without affecting cortisol secretion. in sheep, metoclopramide also stimulates aldosterone but ist action on adrenocortical secretion is more controversial. to clarify the action of metoclopramide in conscious sheep, the response of plasma aldosterone, cortisol, angiotensin ii and potassium were studied after 0.16 and 0.64 mg/kg metoclopramide, with and without pretreatment with dexamethasone. the effect ... | 1987 | 3613572 |
| effect of metoclopramide on adrenal secretion in sheep: influence of dexamethasone and sodium intake. | metoclopramide, a competitive dopamine antagonist, stimulates aldosterone in man and monkey without affecting cortisol secretion. in sheep, metoclopramide also stimulates aldosterone but ist action on adrenocortical secretion is more controversial. to clarify the action of metoclopramide in conscious sheep, the response of plasma aldosterone, cortisol, angiotensin ii and potassium were studied after 0.16 and 0.64 mg/kg metoclopramide, with and without pretreatment with dexamethasone. the effect ... | 1987 | 3613572 |
| the rf1 gene of the killer dna of yeast may encode a dna polymerase. | | 1987 | 3697067 |
| the rf1 gene of the killer dna of yeast may encode a dna polymerase. | | 1987 | 3697067 |
| stereochemistry of estrogen biosynthesis by a reconstituted aromatase cytochrome p-450 preparation from human placenta. | according to the literature, the multistep reaction mechanism of estrogen biosynthesis proceeds with stereospecific loss of the equatorial 1 beta-, and axial 2 beta-protons. these results were deduced from experiments carried out, either with crude microsomes, or at best with impure enzyme extracts. however, when [1,2- 3h]4-androstene-3,17-dione of known absolute 3h-label distribution was incubated with a reconstituted enzyme system, consisting of homogeneous nadph-cytochrome p-450 reductase and ... | 1986 | 3707583 |
| [experimental and clinical studies of sutures of natural silk produced by oak silkworms]. | | 1986 | 3716085 |
| [the development of the zona pellucida in man]. | | 1986 | 3719721 |
| [origin of endometrial granulocytes in human decidual tissue]. | | 1986 | 3772207 |
| new aspects of the epidemiology of hepatitis delta virus infection? | | 1986 | 3804179 |
| functional c8 associated with human platelets. | haemolytic assay for c8 revealed its association in functionally active form with washed human platelets. platelet-bound c8 haemolytic activity was inhibited by f(ab')2 anti-c8 and was undetectable in the platelet suspension obtained from three c8 deficient patients. incubation of platelets from c8 deficient individuals in normal plasma did not restore c8 haemolytic activity, indicating that platelets do not absorb c8 from plasma in vitro during platelet preparation. thrombin, a mediator of the ... | 1986 | 3815901 |
| a new partition thin-layer chromatographic method for steroid separations. | tlc plates with a 25 mu thick polyamide stationary phase were modified for the separation of neutral steroids by impregnation with propylene glycol. a mixture of tritiated 5 alpha-androstan-3 alpha,17 beta-diol, testosterone, 17 beta-hydroxy-5 alpha-androstan-3-one and 4-androstene-3,17-dione was applied to the plate and developed in a toluene mobile phase to a height of 13.6 cm. this resulted in complete resolution of the 4 compounds as detected by a gas flow scanner or imaging analyzer. cuttin ... | 1985 | 3835732 |
| n'-nitrosoanatabine (nat). | | 1985 | 3866744 |
| lambda light chain constant and variable gene complements in wild-derived inbred mouse strains. | previous investigations of lambda light (l) chains in wild-derived inbred (wdi) mouse strains revealed large variations in serum levels of this l chain type as well as differences in antibody responses in which lambda chains predominate. in the present study a diverse group of wdi strains was analyzed by southern blot analysis using probes for v lambda, c lambda 1 and c lambda 2 genes in an attempt to correlate the complement of lambda genes present in these strains with their lambda expression ... | 1985 | 3924628 |
| enkephalin convertase demonstrated in the pituitary and adrenal gland by [3h]guanidinoethylmercaptosuccinic acid autoradiography: dehydration decreases neurohypophyseal levels. | [3h]guanidinoethylmercaptosuccinic acid (gemsa) autoradiography demonstrates the particulate form of a carboxypeptidase b-like peptide processing enzyme, enkephalin convertase (ec 3.4.17.10), in the rat pituitary and adrenal glands. the maximal number of binding sites (bmax) for [3h]gemsa is 20 pmol/mg protein in the intermediate lobe of the pituitary, 12.0 pmol/mg protein in the posterior pituitary lobe, 15 pmol/mg protein in the anterior pituitary lobe, 5.8 pmol/mg protein in the adrenal medul ... | 1985 | 3928337 |
| choroid plexus papilloma containing bone. | the presence of bone in choroid plexus papillomas is a rare event. the authors report a case of choroid plexus papilloma of the ivth ventricle containing bone in a 18-year-old man, and review two previously reported cases, as well as the mechanisms proposed for the presence of bone and cartilage in neuroepithelial neoplasms. | 1985 | 3931408 |
| mechanism of cyclosporin a-induced inhibition of prostacyclin synthesis by macrophages. | in vitro studies of pg production over a 24 h period by adherent rat peritoneal macrophages activated by serum-opsonized zymosan revealed that csa (0.3-10 micrograms/ml) caused a dose-related inhibition of pgi2 (assayed as 6-oxo-pgf1 alpha) formation. indomethacin (ind, 0.01-10 micrograms/ml) and dexamethasone (dex, 0.01-10 micrograms/ml) also inhibited the pg production in a dose-related manner. when arachidonic acid (10 micrograms/ml) was added together with the inhibitors, there was no change ... | 1985 | 3936119 |
| mechanism of cyclosporin a-induced inhibition of prostacyclin synthesis by macrophages. | in vitro studies of pg production over a 24 h period by adherent rat peritoneal macrophages activated by serum-opsonized zymosan revealed that csa (0.3-10 micrograms/ml) caused a dose-related inhibition of pgi2 (assayed as 6-oxo-pgf1 alpha) formation. indomethacin (ind, 0.01-10 micrograms/ml) and dexamethasone (dex, 0.01-10 micrograms/ml) also inhibited the pg production in a dose-related manner. when arachidonic acid (10 micrograms/ml) was added together with the inhibitors, there was no change ... | 1985 | 3936119 |
| intestinal calcium transport in the spontaneously hypertensive rat: response to calcium depletion. | we previously found intestinal ca2+ transport to be lower in the spontaneously hypertensive (sh) as compared with the wistar-kyoto control (wky) rat. these animals were fed a relatively high (1%) ca2+ diet, and the concentration of 1 alpha,25-dihydroxycholecalciferol [1 alpha,25(oh)2d3] in serum was the same in both groups. in the present experiment we tested the possibility that the lower ca2+ transport in the sh rat was the result of unresponsiveness to 1 alpha,25(oh)2d3. we fed diets high and ... | 1986 | 3963190 |
| lactate metabolism by pediococci isolated from cheese. | pediococcus pentosaceus is commonly found among the adventitious microflora of cheddar cheese. when this organism was incubated with l-(+)-lactate under anaerobic conditions, l-(+)-lactate was rapidly converted to d-(-)-lactate until racemic (dl) lactate was present. under aerobic conditions this initial reaction was followed by a slower reaction resulting in the use of both lactate isomers and in the production of acetate and co2. with intact cells the lactate oxidation system had an optimum ph ... | 1985 | 4004222 |
| localization of the spinal accessory motoneurons in the cervical cord in connection with the phrenic nucleus: an hrp study in cats. | the localization of the spinal accessory motoneurons (samns) that innervate the accessory respiratory muscles, the sternocleidomastoid (scm) and trapezius (tp) muscles, was identified in the cat using the horseradish peroxidase (hrp) method. in the cases of hrp bathing of the transected spinal accessory nerve (san), hrp-labeled motoneurons were observed ipsilaterally from the c1 to the rostral c6 segments of the spinal cord. labeled neurons were located principally in the medial and central regi ... | 1985 | 4041873 |
| prolactin in cerebrospinal fluid increases the synthesis and release of hypothalamic gamma-aminobutyric acid. | the effect of intracerebroventricularly (i.v.t.)-injected rat prolactin (2 micrograms/rat) on the function of tuberoinfundibular gamma-aminobutyric acid (gaba)ergic neurones was assessed in adult male rats by measuring the activity of glutamic acid decarboxylase (gad) in the mediobasal hypothalamus (mbh) and the concentrations of gaba in hypophysial portal plasma and in the anterior pituitary gland. fourteen hours after i.v.t. injection of rat prolactin the activity of gad in the mbh was signifi ... | 1985 | 4045339 |
| report of a deletion 11 (qter----q23.3) and short review of the literature. | a male child is described with short stature, mental retardation and unusual facial appearance. cytogenetic analysis revealed a partial deletion of the long arm of chromosome 11: 46,xy,del (11)(qter----q23.3:). a short review of previously reported cases of del 11q is presented. a comparison of the main clinical characteristics and the extent of the 11q deletion is given. | 1985 | 4054171 |
| polyamines and putreanine relax respiratory tract smooth muscle in the guinea-pig. | spermidine, spermine, and putreanine, a metabolite of spermidine, caused transient relaxation of the isolated guinea-pig trachealis. the spermidine effect was not blocked by ouabain, propranolol or indomethacin. spermidine treatment was shown to desensitize the tissue to subsequent additions of spermidine. after exposure to spermidine and repeated washes with spermidine-free solution, trachealis responded to kcl (30 mm) with a paradoxical relaxation. this relaxation was not blocked by propranolo ... | 1985 | 4054214 |
| the effects of vasectomy on the testis. | the increased use of vasectomy for population control has led to a large number of animal studies on the morphologic, physiologic, and immunologic responses to this procedure. the extent, nature, and timing of morphologic alterations in the testis showed marked species variations and ranged from autoimmune orchitis to an absence of detectable changes. physiologic studies indicated, contrary to expectation, that the hydrostatic pressure was elevated only in the distal part of the epididymis and ... | 1985 | 4058509 |
| muscle cross-bridge kinetics in rigor and in the presence of atp analogues. | recently we reported preliminary mechanical experiments on freshly skinned rabbit psoas fibers that suggested that while almost all of the cross-bridges are attached to actin in the presence of 4 mm adenyl-5'-yl-imidodiphosphate (amp-pnp) (ionic strength, 0.13 m), there is an equilibrium between the attached and detached states, so that, in the presence of 4 mm amp-pnp, fibers should not be able to maintain tension (schoenberg, et al., 1984, in contractile mechanisms in muscle, pollack and sugi, ... | 1985 | 4092069 |
| detection of complement activation in human serum using c5a-induced chemiluminescence of human granulocytes. | the complement system can be activated by many factors, including immune complexes, leading to the generation of biologically active split products like c5a anaphylatoxin. this study presents a technique which may be used for measuring c5a activity in human serum. the tetanus-anti-tetanus immune complex and aggregated human igg were used as model activators of the complement cascade. the c5a activity was measured by c5a-induced chemiluminescence of granulocytes; furthermore, a radioimmunoassay w ... | 1985 | 4096529 |
| [anti-arrhythmic action of diazepam, methaqualone and meprobamate]. | | 1970 | 4100561 |
| [production of actinomycin by actinomyces michiganensis strain 538]. | | 1973 | 4130172 |
| [mechanisms of ammonia formation in the brain]. | | 1973 | 4155820 |
| some enzymic properties of human lens esterase i. | | 1974 | 4208803 |
| the iud and prostaglandins: a review of the evidence. | | 1973 | 4209512 |
| [analysis by density gradient centrifugation of dna of e. coli k 12 lacking thymine]. | | 1974 | 4212542 |
| some histochemical reactions in the wall of the arterial vessels of the rabbit after administration of neurohormone-like substances. | | 1970 | 4249031 |
| etiology of congenital heart disease. | | 1970 | 4256571 |
| immunosuppressive treatment of autologous immune complex nephritis in rats. | | 1973 | 4266899 |
| identification of isolates of clostridium perfringens types c and d by agglutination and fluorescent-antibody methods. | agglutination and fluorescent-antibody methods were employed for screening clostridium perfringens types c and d from 393 isolates of this organism. all of 50 strains which were isolated in japan and were agglutinable with an antiserum prepared against a stock strain of type c no. 3182 toxigenically belonged to type c, but the antiserum showed no cross-agglutination with any of type c strains isolated in denmark. all of the latter strains, however, were agglutinated by an antiserum prepared agai ... | 1971 | 4324883 |
| nicotinamide adenine dinucleotide-dependent and nicotinamide adenine dinucleotide-independent lactate dehydrogenases in homofermentative and heterofermentative lactic acid bacteria. | three homofermentative (lactobacillus plantarum b38, l. plantarum b33, pediococcus pentosaceus b30) and three heterofermentative (leuconostoc mesenteroides 39, l. oenos b70, lactobacillus brevis) lactic acid bacteria were examined for the presence or absence of nicotinamide adenine dinucleotide (nad)-dependent and nad-independent d- and l-lactate dehydrogenases. two of the six strains investigated, p. pentosaceus and l. oenos, did not exhibit an nad-independent enzyme activity capable of reducin ... | 1971 | 4333320 |
| [effect of some radiosensitizing agents on the dynamics of 24 na and 42 k in the dog]. | | 1972 | 4341099 |
| infantile herpes zoster after intrauterine exposure to varicella. | | 1973 | 4352725 |
| studies on the lysosomes of l132 cells infected with either rhinovirus type 2 or poliovirus type 1. | | 1974 | 4362408 |
| immunopathogenicity and oncogenicity of murine leukemia viruses. i. induction of immunologic disease and lymphoma in (balb-c times nzb)f1 mice by scripps leukemia virus. | this report clearly demonstrates that a systemic lupus erythematosus (sle)-like syndrome and lymphoma can be induced in immunologically normal (balb/c x nzb)f(1) mice by infection of neonates with a murine leukemia virus (mulv) (scripps leukemia virus [slv] 60a) isolated from nzb lymphoblasts. slv 60a was titered in vitro (xc test) and administered to newborn and adult (balb/c x nzb)f(1) mice over six log(10) dilutions. propagation of mulv in the newborn recipients was indicated by greatly eleva ... | 1974 | 4372290 |
| the enzymatic conversion of sulfoxide to sulfone: the oxidation of methyl tetrahydrofurfuryl sulfoxide to the corresponding sulfone by rat liver microsomes. | | 1967 | 4383217 |
| effects of angiotensin, bradykinin and oxytocin on electrical and mechanical activities in the taenia coli of the guinea pig. | | 1967 | 4384071 |
| [distribution characteristics of nicotinamide coenzymes (nad and nadp) in the cellular structures of the liver and brain following administration of ddt]. | | 1970 | 4393429 |
| the role of the olfactory route on infection of the respiratory tract with venezuelan equine encephalomyelitis virus in normal and operated macaca rhesus monkeys. ii. results of histological examination. | | 1973 | 4405397 |
| editorial: spare the ileum. | | 1974 | 4412991 |
| [toxicity of frigen 113 tr-t]. | | 1974 | 4421072 |
| curvature detectors in human vision? | | 1974 | 4444917 |
| curvature detectors in human vision? | | 1974 | 4444917 |
| [inhibition of cell proliferation in the aortic wall and heart by cinnarizine (stutgeron) in experimental renal hypertension in animal experiments]. | | 1974 | 4450648 |
| adrenergic innervation of the mesentery of the rabbit foetus, observed in stretched preparations by the fluorescence histochemical method. | | 1974 | 4452524 |
| adrenergic innervation of the mesentery of the rabbit foetus, observed in stretched preparations by the fluorescence histochemical method. | | 1974 | 4452524 |
| [effect of fasting and explorative laparotomy on body weight, plasma volume and protein balance in the dog]. | | 1974 | 4458354 |
| gentamicin in the treatment of equine metritis. | | 1972 | 4486548 |
| gentamicin in the treatment of equine metritis. | | 1972 | 4486548 |
| [structural analysis of the internal ear]. | | 1973 | 4540263 |
| oral biology in japan 1973-1974. 2. dental histology. | | 1974 | 4620494 |
| [specific cell-mediated immunity in batrachians. ii. delayed hypersensitivity to bcg in the toad (bufo bufo l.)]. | | 1972 | 4630832 |
| giardia intestinalis. a prospective investigation of the prevalence in denmark. | | 1973 | 4697076 |
| orthopedic problem. | | 1973 | 4761174 |
| some aspects of biotin binding to protein catalyzed by biotin-deficient chicken liver preparations. | | 1974 | 4814148 |